International Journal for Parasitology-Parasites and Wildlife最新文献_第9页

Diversity and distribution of helminth communities of the rodent Akodon montensis Thomas, 1913 (Rodentia: Cricetidae: Sigmodontinae) in preserved and altered environments in the Atlantic Forest, Brazil 巴西大西洋森林保存环境和改变环境中啮齿动物（啮齿目：蟋蟀科：斑齿目）寄生虫群落的多样性和分布

IF 2 3区医学 Q3 ECOLOGY

International Journal for Parasitology-Parasites and Wildlife

Pub Date : 2025-08-01 Epub Date: 2025-06-19 DOI: 10.1016/j.ijppaw.2025.101106

Camila dos Santos Lucio , Thiago dos Santos Cardoso , Rosana Gentile , Viviane Brito Dias , Milton Cezar Ribeiro , Bernardo Rodrigues Teixeira , Paulo Sérgio D'Andrea

The conversion of natural landscapes into agricultural and urban areas results in vegetation loss and habitat fragmentation, affecting biodiversity and favoring generalist species, such as Akodon montensis. This rodent species acts as a reservoir for zoonotic agents and harbors various parasites, including helminths, which are important indicators of environmental changes. The present study analyzed the structure, composition, and diversity of the helminth community of A. montensis in the Atlantic Forest, considering four locations in the state of São Paulo, Brazil. We calculated parasitological parameters (abundance, intensity and prevalence of infection) for each helminth species and evaluated helminth diversity within and between each individual host. In addition, we assessed the influence of type of environment (preserved forest or altered matrix) and host characteristics (sex and body size) on parasitological parameters of helminth species and on helminth diversity. A total of 64 individuals of A. montensis were analyzed, of which 60.9 % were infected with at least one helminth species. Nine species were collected, including eight nematodes and one cestode. No influence of the type of environment and host characteristics was observed on the parasitological parameters of some helminth species (e.g., Protospirura numidica criceticola, Rodentolepis akodontis, Stilestrongylus eta and Syphacia (Syphacia) carlitosi) and on the diversity of helminths within each individual host. A low effect of the type of environment on the diversity of parasites was observed among infracommunities. As an opportunistic species, A. montensis can exploit a wide range of resources and environmental conditions, making it less susceptible to variations in parasite diversity across habitats. These findings reinforce the importance of investigating parasite fauna in different landscapes to understand the impacts of anthropization on host-parasite interactions.

将自然景观转变为农业和城市地区会导致植被丧失和栖息地破碎化，影响生物多样性，并有利于像Akodon montensis这样的多面手物种。这种啮齿类动物是人畜共患病原体的储存库，并藏有各种寄生虫，包括蠕虫，它们是环境变化的重要指标。本研究以巴西圣保罗州的四个地点为研究对象，分析了大西洋森林中A. montensis蠕虫群落的结构、组成和多样性。我们计算了每种寄生虫的寄生虫学参数（丰度、强度和感染流行率），并评估了每个个体宿主内部和宿主之间的寄生虫多样性。此外，我们还评估了环境类型（保留森林或改变基质）和寄主特征（性别和体型）对蠕虫种的寄生参数和寄生虫多样性的影响。共捕获孟氏弓形螨64只，其中60.9%至少感染一种寄生虫。共采集到线虫8种，囊虫1种。环境类型和寄主特征对一些蠕虫物种（如numidica cricticola、roentolepis akodontis、Stilestrongylus eta和Syphacia carlitosi）的寄生参数和每个寄主内蠕虫的多样性没有影响。环境类型对下层群落寄生虫多样性的影响较小。作为一种机会主义物种，孟氏弓形虫可以利用广泛的资源和环境条件，使其不易受不同栖息地寄生虫多样性变化的影响。这些发现加强了在不同景观中调查寄生虫动物群的重要性，以了解人类化对宿主-寄生虫相互作用的影响。

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引用次数: 0

Where there are moose (Alces alces) in Eurasia, there are moose nose botflies: First morphological and molecular identification of Cephenemyia ulrichii (Brauer, 1862) in Kazakhstan 欧亚大陆哪里有驼鹿（Alces Alces），哪里就有驼鹿鼻蝇：在哈萨克斯坦首次发现乌氏鼻蝇（Brauer, 1862）的形态和分子鉴定

IF 2 3区医学 Q3 ECOLOGY

International Journal for Parasitology-Parasites and Wildlife

Pub Date : 2025-08-01 Epub Date: 2025-05-20 DOI: 10.1016/j.ijppaw.2025.101086

Christian Bauer , Rabiga Uakhit , Ainura Smagulova , Karina Jazina , Alexandr Lyalchenko , Vladimir Kiyan

In recent years, the Eurasian moose (Alces alces) population in Kazakhstan has increased significantly. According to 2024 estimates, there are now approximately 8960 individuals, primarily in the northern and eastern regions of the country. However, there is limited information on moose parasites in Kazakhstan. Four of seven moose hunted in the Akmola and Kostanay provinces in July 2023 and May 2024 were found to be infested with 8–12 dipterous fly larvae in the nasopharynx. The larvae were morphologically identified as Cephenemyia ulrichii (Oestridae: Oestrinae) third-instar larvae. Molecular characterization through polymerase chain reaction amplification and partial sequencing of the mitochondrial cytochrome oxidase subunit I gene from the larvae showed that it was 99 % identical to a reference C. ulrichii isolate from Sweden. The gene sequences of the Kazakh isolates clustered in a clade with the reference sample, distinct from other Cephenemyia species. This is the first report of C. ulrichii nasopharyngeal myiasis in moose in Kazakhstan, marking the first such report in Central Asia and expanding the known geographical range of this parasite.

近年来，哈萨克斯坦的欧亚驼鹿（Alces Alces）数量显著增加。根据2024年的估计，目前约有8960人，主要分布在该国北部和东部地区。然而，关于哈萨克斯坦驼鹿寄生虫的信息有限。在2023年7月和2024年5月在Akmola和Kostanay省捕获的7只驼鹿中，有4只在鼻咽部发现了8-12只双翅蝇幼虫。经形态学鉴定，该幼虫为乌氏赤霉（赤霉科：赤霉科）三龄幼虫。通过聚合酶链反应扩增和线粒体细胞色素氧化酶亚基I基因的部分测序，对该幼虫进行了分子鉴定，结果表明，该幼虫与瑞典乌氏弓形虫参考分离物的同源性为99%。哈萨克分离株的基因序列与参考样本聚集在一个进化枝上，与其他头孢菌属不同。这是哈萨克斯坦驼鹿中首次报告乌氏弓形虫鼻咽蝇病，标志着中亚地区首次此类报告，并扩大了该寄生虫已知的地理范围。

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引用次数: 0

Morphological and phylogenetic comparison of two myxozoan parasites infecting the gills of pikeperch (Sander lucioperca L.): Henneguya gigantea and Henneguya creplini 侵染棘鲈鳃的两种黏液动物寄生虫的形态和系统发育比较：巨棘鲈和克雷普里鲈

IF 2 3区医学 Q3 ECOLOGY

International Journal for Parasitology-Parasites and Wildlife

Pub Date : 2025-08-01 Epub Date: 2025-05-26 DOI: 10.1016/j.ijppaw.2025.101087

Graciela Colunga-Ramírez , Boglárka Sellyei , Kálmán Molnár , Csaba Székely , Gábor Cech

Henneguya gigantea and Henneguya creplini have been reported as spore-forming plasmodia infecting the gills of pikeperch. However, the latter has been documented to have a broader host range. Some remarkable differences have been observed between both Henneguya species: the shape and size of plasmodia and the length of their caudal appendages. To date, no molecular evidence has been provided for H. gigantea; while for H. creplini, the 18S rDNA has been applied for its classification. In this study, we analysed morphological and molecular data to elucidate the relationship of H. gigantea and H. creplini collected from the gills of the pikeperch. Morphologically, H. gigantea resulted in a cluster of plasmodia-forming cyst bodies, while H. creplini have been mostly reported as small plasmodia. The myxospores of the two species were distinguished by the length of their caudal appendages, which were longer in H. gigantea than in H. creplini (77–100 vs 14.4–40.1 μm). The rest of the spore characters are similar in both species. The 18S and 28S rDNA sequences show low sequence distance ≤0.76 % and 0.97 %, respectively. The data presented in this study, in conjunction with those collected from previous literature, suggests that caudal appendages may exhibit phenotypic plasticity within species, which must be considered before including new members of the Henneguya genus. Furthermore, ribosomal DNA sequences serve as an essential tool for referring the taxonomic classification of myxozoans that exhibit phenotypic plasticity. We propose that H. gigantea be reclassified as H. creplini.

据报道，巨型亨尼古雅和克雷普里尼亨尼古雅是感染猪鳃的孢子形成疟原虫。然而，根据文献记载，后者具有更广泛的宿主范围。在两种亨内古雅虫之间观察到一些显著的差异：疟原虫的形状和大小以及尾肢的长度。到目前为止，还没有提供关于H. gigantea的分子证据；而对于H. creplini，则采用18S rDNA进行分类。本研究通过形态学和分子分析资料，阐明了从棘鲈鳃中采集的H. gigantea和H. creplini的亲缘关系。在形态学上，巨芽胞杆菌形成了一簇形成疟原虫的囊体，而克雷普利尼芽胞杆菌则多为小的疟原虫。两种黏液孢子的黏液孢子的尾端附属物长度可以区分为：H. gigantea比H. creplini长（77 ~ 100 μm比14.4 ~ 40.1 μm）。其余孢子性状在两种中相似。18S和28S rDNA序列序列距离较低，分别≤0.76%和0.97%。本研究中提出的数据，结合从以前的文献中收集的数据，表明尾肢可能在物种内表现出表型可塑性，这在包括Henneguya属的新成员之前必须考虑。此外，核糖体DNA序列作为参考黏液动物的分类分类的重要工具，表现出表型可塑性。我们建议将H. gigantea重新分类为H. creplini。

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引用次数: 0

Molecular detection of Leishmania and other vector-borne agents in free-ranging and captive herpetofauna from Costa Rica 哥斯达黎加自由放养和圈养疱疹动物中利什曼原虫和其他媒介传播媒介的分子检测

IF 2 3区医学 Q3 ECOLOGY

International Journal for Parasitology-Parasites and Wildlife

Pub Date : 2025-08-01 Epub Date: 2025-05-27 DOI: 10.1016/j.ijppaw.2025.101090

Mario H. Alves , Jairo Alfonso Mendoza-Roldan , Paula Alfaro-Segura , Mariaelisa Carbonara , Aarón Gómez , Natalia Montero Leitón , Jazmín Arias Ortega , Alberto Solano-Barquero , Alicia Rojas , Domenico Otranto

Vector-borne pathogens in amphibians and reptiles represent an emerging concern in wildlife, with implications for ecosystem dynamics and potential zoonotic risks. In this study, we screened 108 animals from Costa Rica, including 46 captive snakes, 24 free-ranging reptiles, and 38 free-ranging amphibians, for the presence of Trypanosomatidae, Anaplasmataceae, Borrelia, Rickettsia, and Hepatozoon spp. Blood smear analysis revealed protozoa gametocytes in 3.7 % of the animals sampled, and 11.1 % of amphibians and reptiles were molecular positive for at least one pathogen. Specifically, 7.4 % of the samples tested positive for Leishmania spp., 1.85 % for Trypanosoma spp., 0.9 % for Anaplasma spp., and 1.85 % for Hepatozoon spp. Notably, this study reports the first molecular detection of Leishmania in an amphibian species (Rhinella horribilis) and confirms the presence of mammalian pathogenic Leishmania infantum in captive snakes in Central America. The presence of potential zoonotic agents in both captive and free-ranging herpetofauna underscores the importance of screening wildlife species, including understudied host groups such as amphibians, to better understand their role in disease ecology.

两栖动物和爬行动物中媒介传播的病原体是野生动物中一个新出现的问题，对生态系统动力学和潜在的人畜共患风险具有影响。在这项研究中，我们筛选了来自哥斯达黎加的108只动物，包括46只圈养蛇，24只自由放养的爬行动物和38只自由放养的两栖动物，检测了锥虫科、无形体科、伯氏疏螺旋体、立克次体和肝虫等动物。血液涂片分析显示，3.7%的动物样本中有原生动物配子体，11.1%的两栖动物和爬行动物至少有一种病原体分子阳性。具体来说，7.4%的样本检测利什曼原虫阳性，1.85%的样本检测锥虫阳性，0.9%的样本检测无形体阳性，1.85%的样本检测肝虫阳性。值得注意的是，本研究报告了首次在两栖动物物种（可怕的鼻虫）中检测到利什曼原虫的分子，并证实了中美洲圈养蛇中存在哺乳动物致病性幼利什曼原虫。圈养和自由放养的爬行动物中都存在潜在的人畜共患病原体，这强调了筛选野生动物物种的重要性，包括两栖动物等尚未得到充分研究的寄主群体，以便更好地了解它们在疾病生态学中的作用。

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引用次数: 0

Trypanosoma cruzi in wild mammals from an endemic area of Chagas disease on the coast of Ecuador 厄瓜多尔海岸恰加斯病流行区野生哺乳动物中的克氏锥虫

IF 2 3区医学 Q3 ECOLOGY

International Journal for Parasitology-Parasites and Wildlife

Pub Date : 2025-08-01 Epub Date: 2025-04-18 DOI: 10.1016/j.ijppaw.2025.101073

Ana Soto-Vivas , Alexander Nicolalde , María Mercedes Gavilanez , Juan Carlos Benalcázar , Camila Acosta-López , Jhocelyn Chiluiza , María Isabel Calvopiña , Édison Encalada , Germán Jacóme , Jonathan Liria

Trypanosoma cruzi is a protozoan parasite that causes Chagas disease, endemic in Ecuador. In the province of Los Ríos, where the vector Triatoma dimidiata is present, vector-borne transmission is considered the primary route of infection. Many mammalian are involved in the transmission cycle of T. cruzi. Nonetheless, significant gaps remain regarding mammalian reservoirs along the Ecuadorian coast, especially in ecologically altered areas. To investigate the role of wild and domestic mammals as hosts, we assessed the presence of T. cruzi in two localities of Quevedo canton. Sampling focused on wild, domestic, and peridomestic mammals using live capture techniques such as mist nets for bats and Sherman and Tomahawk traps for terrestrial mammals. Blood samples were collected from all individuals, and DNA was extracted using a commercial kit. PCR was performed targeting three regions of the T. cruzi genome. In total, 383 mammals were sampled: 66 domestic animals, 6 peridomestic, and 317 wild individuals. Of the wild mammals, 216 were captured in La Virginia 2 and 95 in the Jacome Forest. Four wild individuals tested positive for T. cruzi, including three Glossophaga soricina and one Marmosa simonsi, all collected from La Virginia 2, a peri-urban community. None of the domestic or peridomestic animals were infected. This study reports, for the first time, the infection of M. simonsi with T. cruzi. The presence of positive cases exclusively in an area close to human settlements raises concern about the risk of parasite transmission in transitional landscapes. Transmission cycles of the parasite are known to be influenced by land-use change, deforestation, and host community composition. These factors may alter ecological interactions between vectors and hosts, potentially favoring transmission. Further research is needed in Ecuador to understand how ecosystem alterations shape the sylvatic cycle of T. cruzi, particularly the role of bats in disease maintenance.

克氏锥虫是一种引起厄瓜多尔地方病恰加斯病的原生动物寄生虫。在Los Ríos省，存在双口三角瘤病媒，病媒传播被认为是主要感染途径。许多哺乳动物参与克氏锥虫的传播周期。尽管如此，在厄瓜多尔海岸哺乳动物水库方面，特别是在生态改变地区，仍然存在重大差距。为了调查野生和家养哺乳动物作为宿主的作用，我们在克维多州的两个地方评估了克氏锥虫的存在。采样重点是野生、家养和家养哺乳动物，使用现场捕获技术，如对蝙蝠使用雾网，对陆生哺乳动物使用谢尔曼和战斧陷阱。收集所有个体的血液样本，并使用商业试剂盒提取DNA。针对克氏T. crozi基因组的三个区域进行PCR。共采集哺乳动物383只，其中家畜66只，圈养动物6只，野生动物317只。在野生哺乳动物中，216只在拉维吉尼亚2号捕获，95只在雅克姆森林捕获。4只野生个体的克氏体检测呈阳性，包括3只舌蝗和1只猴腹蝗，均采集于近郊社区La Virginia 2。所有家畜和围家畜均未感染。本研究首次报道了西蒙西氏分枝杆菌与克氏锥虫的感染。阳性病例仅在靠近人类住区的地区出现，这引起了人们对过渡景观中寄生虫传播风险的关注。已知寄生虫的传播周期受土地利用变化、森林砍伐和寄主群落组成的影响。这些因素可能改变病媒与宿主之间的生态相互作用，可能有利于传播。厄瓜多尔需要进一步研究，以了解生态系统变化如何影响克氏锥虫的森林循环，特别是蝙蝠在疾病维持中的作用。

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引用次数: 0

Sarcocystis spp. and Toxoplasma gondii in muscles from wild boars (Sus scrofa) consumed in Switzerland 瑞士食用野猪（Sus scrofa）肌肉中的肌囊虫和刚地弓形虫

IF 2 3区医学 Q3 ECOLOGY

International Journal for Parasitology-Parasites and Wildlife

Pub Date : 2025-08-01 Epub Date: 2025-07-09 DOI: 10.1016/j.ijppaw.2025.101114

Moré Gastón , Filippini Carlotta , Andreas W. Oehm , Ruetten Maja , Hemphill Andrew , Caroline F. Frey , Basso Walter

Sarcocystis spp. and Toxoplasma gondii are heteroxenous protozoan parasites, which form cysts in muscle tissue of intermediate hosts (IH) and oocysts in the intestinal mucosa of definitive hosts (DH). Wild boars (Sus scrofa) are IH for Sarcocystis miescheriana (with wild and domestic canids as DH), S. suihominis (with humans and non-human primates as DH) and T. gondii (with felids as DH). The aim of this study was to identify and characterize Sarcocystis spp. and T. gondii in the muscle tissue of wild boars hunted and/or consumed in Switzerland. DNA was extracted from muscle samples of 286 wild boars and tested by PCRs targeting the 18S rRNA gene of Sarcocystis spp., the mitochondrial cytochrome c oxidase (cox1) gene of S. suihominis and by a qPCR targeting a 529 bp genomic repeat of T. gondii. Besides, 225 samples were processed by homogenization and direct microscopic examination and 42 samples by histopathology. Sarcocysts were detected microscopically in 89.3 % (201/225) and 35.7 % (15/42) of the samples, respectively. The 18S rRNA PCR detected parasite DNA in 91.3 % (261/286) of the samples, and all 62 sequences obtained were 100 % identical, and also with GenBank sequences reported as S. miescheriana, suggesting a high environmental contamination with canid faeces. The S. suihominis-cox1 PCR was positive in 1.75 % of the samples (5/286), and the six cox1 sequences (772 bp primers trimmed) obtained were 97.3–99.6 % similar among them and 97.7–100 % identical with a sequence reported as S. suihominis. Despite the identity differences, all these sequences were grouped together in a phylogenetic tree, in a sister clade of S. miescheriana sequences. All samples tested negative for T. gondii by qPCR. This study revealed for the first time the presence of S. suihominis in wild boars hunted in Switzerland.

Sarcocystis spp.和弓形虫（Toxoplasma gondii）是异种原生动物寄生虫，它们在中间宿主（IH）的肌肉组织中形成囊肿，在最终宿主（DH）的肠粘膜中形成卵囊。野猪（Sus scrofa）是米氏肉囊菌（以野生和家养犬科动物为DH）、猪链球菌（以人类和非人类灵长类动物为DH）和弓形虫（以猫科动物为DH）的DH。本研究的目的是鉴定和表征在瑞士狩猎和/或消费的野猪肌肉组织中的肌囊虫和弓形虫。从286只野猪的肌肉样本中提取DNA，分别以肉囊虫18S rRNA基因、猪链球菌线粒体细胞色素c氧化酶（cox1）基因和弓形虫529 bp基因组重复序列为目标进行pcr检测。225例经均质及直接显微镜检查，42例经组织病理学检查。显微镜下检出结节囊肿的比例分别为89.3%（201/225）和35.7%（15/42）。18S rRNA PCR检测到91.3%（261/286）的寄生虫DNA，获得的62条序列完全相同，且与GenBank报道的S. miescheriana序列一致，提示该虫与犬科动物粪便污染严重。1.75%的样本（5/286）的cox1 PCR阳性，得到的6条cox1序列（772 bp的引物）相似度为97.3 - 99.6%，与已报道的一条序列相同度为97.7 - 100%。尽管存在身份差异，但所有这些序列都被归类在一个系统发育树中，属于S. miescheriana序列的姐妹分支。所有样本经qPCR检测均为弓形虫阴性。这项研究首次揭示了在瑞士猎杀的野猪中存在猪链球菌。

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引用次数: 0

Fatal babesiosis in a free-ranging iberian wolf co-infected with Hepatozoon canis and Leishmania infantum: Epidemiological implications for the cantabrian wolf population 同时感染犬肝虫病和幼利什曼原虫的自由放养伊比利亚狼的致命巴贝斯虫病：对伊比利亚狼种群的流行病学意义

IF 2 3区医学 Q3 ECOLOGY

International Journal for Parasitology-Parasites and Wildlife

Pub Date : 2025-08-01 Epub Date: 2025-05-13 DOI: 10.1016/j.ijppaw.2025.101080

Manena Fayos , Natalia Sastre , Ana M. Palomar , Carlos Sacristán , Irene Sacristán , Ana Carolina Ewbank , Emmanuel Serrano , Roser Velarde

Health surveillance of large carnivores, such as the Iberian wolf (Canis lupus signatus), allows the early detection of emerging and established pathogens in natural environments, due to their position in the top of the food chain. In 2022, the carcass of a wild wolf was found in Cantabria, Northwest Spain. A complete post-mortem investigation, including full necropsy, histopathological and molecular analysis concluded that the cause of death was a systemic hemolytic disease caused by Babesia canis. Furthermore, the wolf was co-infected with Hepatozoon canis and Leishmania infantum. Consequently, a molecular investigation of Babesia spp., Hepatozoon spp., Leishmania spp., Ehrlichia spp., Anaplasma spp., and Rickettsia spp. was performed in the Cantabrian wolf population (n = 91). The prevalences found were 6.3 % (5/79) for B. canis, 96.3 % (78/81) for H. canis, and 23.8 % (21/88) for L. infantum. None of the wolves were positive to Ehrlichia spp., Anaplasma spp., or Rickettsia spp. The co-infection prevalences were also investigated. To our knowledge, this is the first description of a fatal babesiosis in a wild wolf, as well as the first detection of B. canis and H. canis infection in the Iberian wolf population. We have found a low B. canis prevalence, while the H. canis prevalence is the highest recorded so far. The detection of L. infantum highlights the circulation of this zoonotic pathogen in a non-endemic region.

对伊比利亚狼（Canis lupus signatus）等大型食肉动物的健康监测，由于它们处于食物链的顶端，可以在自然环境中早期发现新出现的和已建立的病原体。2022年，在西班牙西北部的坎塔布里亚发现了一只野狼的尸体。完整的尸检调查，包括全面尸检、组织病理学和分子分析，得出的结论是，死亡原因是由犬巴贝斯虫引起的全身性溶血性疾病。此外，狼同时感染了犬肝虫和幼利什曼原虫。因此，我们对坎塔布里亚狼种群（n = 91）进行了巴贝斯虫、肝虫、利什曼原虫、埃利希氏体、无形体和立克次体的分子调查。犬b型、犬h型和婴幼儿l型的感染率分别为6.3%（5/79）、96.3%（78/81）和23.8%（21/88）。所有狼均未检出埃利希体、无形体和立克次体，并调查了它们的共感染流行情况。据我们所知，这是首次在野狼中发现致命性巴贝斯虫病，也是首次在伊比利亚狼种群中发现犬b型和犬h型感染。我们发现犬b患病率较低，而犬h患病率是迄今为止记录的最高水平。婴儿乳杆菌的检测突出了这种人畜共患病原体在非流行地区的传播。

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引用次数: 0

Influence of latitude, host body size and host body weight on helminth species richness and abundance in two Neotropical marsupials 纬度、寄主体型和寄主体重对两种新热带有袋类蠕虫物种丰富度和丰度的影响

IF 2 3区医学 Q3 ECOLOGY

International Journal for Parasitology-Parasites and Wildlife

Pub Date : 2025-08-01 Epub Date: 2025-05-06 DOI: 10.1016/j.ijppaw.2025.101077

Bruna Silva Cirino , Sócrates Fraga da Costa Neto , Thiago dos Santos Cardoso , Arnaldo Maldonado Júnior , Rosana Gentile

Parasite species richness can be influenced by intrinsic and extrinsic factors of their hosts, such as host body size and latitude. Although these factors have been studied for several taxa, few studies have investigated them for helminths of wild mammals. The aim of this study was to evaluate the influence of host body size, body weight and latitude on the species richness and abundance of the helminths of Didelphis aurita and Didelphis albiventris. Data on helminths and the geographic coordinates of the collection localities of the hosts were obtained from the Collection of Wild Mammal Reservoirs and the database of the Laboratory of Biology and Parasitology of Wild Mammal Reservoirs. The influences of latitude and host body size and body weight on helminth species richness (HSR) and helminth abundance were analysed using linear regressions. We evaluated the effect of latitude on helminth species composition using redundancy analysis followed by ANOVA. The HSR ranged from 1 to 9 for D. aurita and from 1 to 7 for D. albiventris. We found a positive relationship between latitude and total HSR for D. aurita (p = 0.012). The ANOVA revealed the influence of latitudinal variation on the species composition variation of helminths only for D. aurita (p = 0.001). With respect to body size, we did not find a significant relationship between this variable and HSR or abundance for either species. However, we found a positive relationship between host body weight and helminth abundance for D. aurita (p = 0.004). We conclude that the increase in latitude was an explanatory factor for the increasing HSR along infracommunities, contradicting the general pattern of increasing species with decreasing latitude observed in free-living species. Moreover, host intrinsic factors may be more relevant to endoparasite occurrence and development than latitude because they directly influence the parasite niche.

寄生物种丰富度受寄主的内在和外在因素的影响，如寄主的体型和纬度。虽然这些因素已经在一些分类群中进行了研究，但很少有研究对野生哺乳动物的蠕虫进行了调查。本研究的目的是评价寄主体型、体重和纬度对白腹蓟马（Didelphis aurita）和白腹蓟马（Didelphis albiventris）蠕虫种类丰富度和丰度的影响。寄生虫数据和寄主采集地地理坐标来源于《野生哺乳动物库》和《野生哺乳动物库生物学与寄生虫学实验室》数据库。采用线性回归分析了纬度和寄主体大小、体质量对线虫物种丰富度和丰度的影响。我们使用冗余分析和方差分析来评估纬度对蠕虫物种组成的影响。aurita的HSR为1 ~ 9，albiventris的HSR为1 ~ 7。我们发现纬度与aurita的总HSR呈正相关（p = 0.012）。方差分析显示，纬度变化只对乌氏夜蛾的种类组成变化有影响（p = 0.001）。在体型方面，我们没有发现这一变量与两种物种的HSR或丰度之间存在显著关系。然而，我们发现寄主体重与aurita寄生虫丰度呈正相关（p = 0.004）。我们认为纬度的增加是基础群落高通量增加的一个解释因素，这与自由生活物种随纬度的降低而增加的一般模式相矛盾。此外，与纬度相比，宿主内在因素可能与寄生虫的发生和发展更为相关，因为它们直接影响寄生虫的生态位。

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引用次数: 0

Molecular identification of Trichuris species in long-tailed macaques from Dong Ling Don Chao Pu Park and Kumphawapi Monkey Garden, Northeast Thailand: First report suggesting possible Trichuris ovis infection in non-human primates 泰国东北东陵东朝浦公园和Kumphawapi猴园长尾猕猴中毛滴虫的分子鉴定：首次提示非人类灵长类动物中可能存在毛滴虫感染

IF 2 3区医学 Q3 ECOLOGY

International Journal for Parasitology-Parasites and Wildlife

Pub Date : 2025-08-01 Epub Date: 2025-04-01 DOI: 10.1016/j.ijppaw.2025.101063

Issarapong Phosuk , Tongjit Thanchomnang , Julalak Banglua , Sakhone Laymanivong , Darunee Puangpronpitag , Jurairat Jongthawin

This study aimed to molecularly identify Trichuris spp. in long-tailed macaques from two key habitats in Northeast Thailand: Dong Ling Don Chao Pu Park, Amnat Charoen Province, and Kumphawapi Monkey Garden, Udon Thani Province. Genomic DNA was extracted from 13 Trichuris spp. egg samples collected from 13 infected long-tailed macaques, and PCR amplification targeting partial sequences of the 18S rRNA gene and ITS2 region was performed for phylogenetic analysis. Of the 13 Trichuris spp. egg samples, the partial 18S rRNA gene was successfully amplified from six, while ITS2 amplification was unsuccessful. Phylogenetic analysis indicated that four specimens sequenced from Dong Ling Don Chao Pu Park were T. trichiura. In contrast, two specimens sequenced from Kumphawapi Monkey Garden clustered with the only confirmed T. ovis from goat, as well as unconfirmed Trichuris spp. from other ruminant hosts. These findings suggest that the Trichuris spp. in macaques are likely T. ovis; however, the evidence remains inconclusive. Therefore, accurate species identification in this region requires further molecular analysis using additional genetic markers. This study provides the first molecular identification of T. trichiura in long-tailed macaques from Northeast Thailand. Additionally, it is the first report suggesting the possible T. ovis infection in non-human primates. These findings highlight the potential for Trichuris spp. transmission across diverse host species, underscoring the need for enhanced surveillance of parasitic infections in wildlife and livestock, particularly in regions with close human-animal interactions. Continued molecular investigations are essential to elucidate Trichuris spp. transmission dynamics and zoonotic potential, aiding in public health risk mitigation.

本研究旨在对泰国东北部两个主要栖息地——Amnat Charoen省Dong Ling Don Chao Pu公园和Udon Thani省Kumphawapi Monkey Garden的长尾猕猴进行Trichuris spp.的分子鉴定。从13只感染长尾猕猴的13只毛猴卵中提取基因组DNA，对18S rRNA基因和ITS2区域的部分序列进行PCR扩增，进行系统发育分析。在13份毛滴虫卵中，6份成功扩增到部分18S rRNA基因，而ITS2基因扩增失败。系统发育分析表明，从东陵东朝铺公园获得的4份标本均为毛毛蝗。相比之下，来自Kumphawapi猴园的两个标本与来自山羊的唯一确认的鹅绦虫以及来自其他反刍动物宿主的未经确认的毛线虫属聚集在一起。这些发现表明猕猴体内的毛滴虫很可能是T. ovis；然而，证据仍然没有定论。因此，准确的物种鉴定需要使用额外的遗传标记进行进一步的分子分析。本研究首次在泰国东北部的长尾猕猴中鉴定出毛螺旋体。此外，这是首次提出在非人类灵长类动物中可能存在卵巢绦虫感染的报告。这些发现突出了毛滴虫在不同宿主物种间传播的可能性，强调了加强对野生动物和牲畜中寄生虫感染监测的必要性，特别是在人与动物密切互动的地区。继续进行分子调查对于阐明毛滴虫的传播动力学和人畜共患的可能性至关重要，有助于减轻公共卫生风险。

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引用次数: 0

Different age, different blood parasites - Acrocephalus species and their haemosporidian parasites during autumn migration in Central Europe 不同的年龄，不同的血寄生虫——中欧秋季迁徙中的顶头虫及其血孢子虫

IF 2 3区医学 Q3 ECOLOGY

International Journal for Parasitology-Parasites and Wildlife

Pub Date : 2025-08-01 Epub Date: 2025-06-01 DOI: 10.1016/j.ijppaw.2025.101085

Nóra Ágh , Szilvia Pásztory-Kovács , Viola Prohászka , Tibor Csörgő , Eszter Szöllősi

In migratory passerines, the timing of the different phases of the migratory journey is of great importance for the survival prospect of the individuals. Haemosporidian infections could weaken the immune system, decelerate the ability of fat accumulation and potentially influence the migratory behaviour. As a large number of blood parasites is host-generalist there is a potential for cross-species and cross-population transmission with different parasite species during the migratory route of the passerines. In addition, resident birds also interact with different parasite fauna when migratory birds arrive back from their migratory route, therefore, it is important to study what kind of blood parasites migratory birds carry.

We screened three long-distant migratory Acrocephalus species during autumn migration in two years. We found that in reed and sedge warblers the overall prevalence of blood parasites was significantly higher in adults than in juveniles, and the prevalence of Haemoproteus infections was higher than that of the Plasmodium in adults. In contrast, Plasmodium infections dominated in juveniles in all the three species. The odds of catching infected juvenile individuals increased during the autumn migration, but infections had no significant effect on the actual body mass of the birds. These results could imply age-related differences in the probability of getting infected with different blood parasite genera. Sampling during migration and exploring the potential differences in parasite species composition and their effects on the migratory behaviour in different age groups can provide valuable insight in answering these questions.

在迁徙的雀形目动物中，不同迁徙阶段的时间对个体的生存前景至关重要。血孢子虫感染可以削弱免疫系统，减缓脂肪积累的能力，并可能影响迁移行为。由于大量血寄生虫具有寄主通用性，因此在雀形目动物的迁徙路线中，存在与不同寄主物种跨种、跨种群传播的可能性。此外，当候鸟从迁徙路线返回时，留鸟还与不同的寄生动物群相互作用，因此研究候鸟携带了何种血寄生虫具有重要意义。在两年的秋季迁徙中，我们筛选了三种远距离迁徙的头甲动物。结果表明，芦苇和莎草莺血寄生虫的总体流行率在成虫中明显高于幼虫，且嗜血杆菌感染的流行率高于疟原虫。相比之下，在所有三个物种的幼体中，疟原虫感染占主导地位。在秋季迁徙期间，捕获受感染的幼鸟的几率增加了，但感染对鸟类的实际体重没有显著影响。这些结果可能意味着感染不同血液寄生虫属的可能性存在与年龄相关的差异。在迁徙过程中进行采样，探索不同年龄段寄生虫物种组成的潜在差异及其对迁徙行为的影响，可以为回答这些问题提供有价值的见解。

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引用次数: 0