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STATISTICAL TRENDS OF THE TOURISM INDUSTRYIN CALARASI COUNTY, ROMANIA 罗马尼亚卡拉拉西县旅游业统计趋势
IF 0.9 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2022-07-22 DOI: 10.24818/cts/4/2022/1.05
C. Mirea
Tourist establishments are indispensable elements of touristic activity, in the sense that the tourism potential of adestination cannot be capitalized without the existence of accommodation, food, transport and leisureestablishments, but also of travel agencies. Calarasi County has all the tourism establishments mentioned above,and their dynamics are different, presenting different growth rates. The purpose of this work is to carry out astatistical analysis of the aforementioned tourist establishments in order to find out whether they tend to increaseor decrease their number in the future. In the case of Calarasi County, besides the transport establishments, theother tourism establishments analyzed show a tendency to increase from a numerical point of view, a factdemonstrated by the adjustment of the data series. For the processing of the data, average indicators, indicatorsof the central trend were used, and for the adjustment of the data, the method of the average increase was used.
旅游设施是旅游活动中不可缺少的要素,也就是说,如果没有住宿、饮食、交通和休闲设施以及旅行社的存在,一个目的地的旅游潜力就不能得到充分利用。卡拉拉西县拥有上述所有旅游机构,其动态不同,呈现不同的增长速度。这项工作的目的是对上述旅游机构进行统计分析,以了解它们在未来的数量是有增加还是减少的趋势。就卡拉拉西县而言,除了交通设施外,从数字的角度来看,分析的其他旅游设施也有增加的趋势,这一事实可以通过数据序列的调整来证明。数据处理采用平均指标、中心趋势指标,数据调整采用平均增幅法。
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引用次数: 0
SPACE TOURISM. A FUTURE SUSTAINABLE BUSINESS OR NOT? 太空旅游。未来是否可持续发展?
IF 0.9 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2022-07-22 DOI: 10.24818/cts/4/2022/1.06
R. Baciu
Space tourism, which has recently come to the attention of society, has sparked controversy over the environmentalconsequences of recreational space travel, which has become a lucrative business done by and for the super-rich. Thisscientific paper investigates, through qualitative and quantitative research, the potential for the success andsustainability of this industry. The interviewed tourism and aviation experts believe that space tourism deserves to bedeveloped in order to colonise other planets and make society responsible for the environmental issues, despite thehuge costs, which will be reduced, similar to the aviation trend, by technological advances. Also, the results of thesurvey in which students participated, as future specialists in business and tourism, indicate an increased share ofthose aware of the effects of carbon pollution and a percentage of over 30% of those who believe in the developmentof space tourism, supported by economic benefits of offsetting environmental costs. The overall conclusion of the studyhighlights technological progress as a mobilising factor in reducing carbon dioxide emissions from spacecraft and inachieving the dream of sustainability of space travel, revealing space tourism as a resource that would benefit societyas a whole, scientifically and economically.
太空旅游最近引起了社会的关注,它引发了关于娱乐性太空旅行对环境影响的争议,这已经成为一项由超级富豪从事和为超级富豪服务的有利可图的生意。这篇科学论文通过定性和定量研究,调查了这个行业成功和可持续发展的潜力。受访的旅游和航空专家认为,为了殖民其他星球,让社会对环境问题负责,太空旅游值得发展,尽管成本巨大,但与航空趋势类似,技术进步会降低成本。此外,作为未来商业和旅游业专家的学生参与的调查结果表明,意识到碳污染影响的比例有所增加,超过30%的人相信空间旅游的发展可以抵消环境成本的经济效益。该研究的总体结论强调了技术进步是减少航天器二氧化碳排放和实现太空旅行可持续性梦想的一个动员因素,揭示了太空旅游作为一种资源将使整个社会在科学和经济上受益。
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引用次数: 0
EDUCATIONAL TOURISM AND LOCAL DEVELOPMENT 教育旅游和地方发展
IF 0.9 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2022-07-22 DOI: 10.24818/cts/4/2022/1.02
Marta-Christina Suciu, Marco Savastano, Gheorghe-Alexandru Stativă, I. Gorelova
Educational tourism is a new branch of tourism, which has experienced a significant increase in recent times, dueto the important benefits it brings to the local communities. Universities and other educational specializedinstitutions may highly contribute, support and encourage local development through educational tourism.Students who benefit from studying abroad can be considered as symbolic educational tourists, and their stay isconsidered to have a high potential to generate benefits. The host university may facilitate the development ofbetter relationships between tourists and local communities in order to contribute to the development of the localeconomy in a sustainable and resilient way. Education tourism has to be taken into account by all educationalinstitutions due to the different learning opportunities that might be better exploited. This paper focuses on someindicators considered to be relevant for educational tourism.
教育旅游是旅游业的一个新兴分支,由于它给当地社区带来了重要的利益,近年来经历了显著的增长。大学和其他教育专业机构可以通过教育旅游大力促进、支持和鼓励地方发展。从留学中受益的学生可以被视为象征性教育游客,他们的逗留被认为具有很高的产生效益的潜力。主办大学可以促进游客和当地社区之间更好的关系的发展,从而以可持续和有弹性的方式为当地经济的发展做出贡献。教育旅游必须考虑到所有的教育机构,因为不同的学习机会,可能会更好地利用。本文重点讨论了与教育旅游相关的一些指标。
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引用次数: 5
Physical-chemical quality of xoconostle fruits (Opuntia matudae and O. joconostle) in the Valle del Mezquital, Hidalgo, Mexico 墨西哥伊达尔戈梅兹奎塔尔山谷软果(Opuntia matudae和O. joconostle)的理化品质
IF 0.9 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2022-06-30 DOI: 10.56890/jpacd.v24i.505
Mónica Gutiérrez-Rojas Mónica Gutiérrez-Rojas, Daniel Ruiz-Juárez Daniel Ruiz-Juárez, Gilberto Vela-Correa Gilberto Vela-Correa, Javier L Olivares-Orozco Javier L Olivares-Orozco, E. Rueda-Puente
The physical and chemical parameters of the soils are decisive in the physiological development of plants of agricultural interest. In the case of some species of Opuntia called xoconostle, the impact of the physical and chemical quality of the fruits, on the soils where they are grown, is unknown. The objective of this study was to determine the physical-chemical quality of two xoconostles fruits (Opuntia matudae cv. Cuaresmero and O. joconostle cv. Burro) cultivated and wild in the Valle del Mezquital, Hidalgo, Mexico. The physical and chemical characteristics of soils and fruits of xoconostle O. matudae and O. joconostle cultivated and wild in the state of Hidalgo, Mexico were analyzed with five repetitions. The data were treated with the JMP V8 program, expressed as mean ± standard deviation. The differences among the means of the fruits were treated with analysis of variance (ANOVA) with a significance level of P?0.001. The differences among the means were evaluated with the Tukey test with a significance level of (P?0.05). Among the physical and chemical properties of the soils, they presented a pale brown (10YR 6/3), with higher porosity, more acidic pH, and exchangeable ions, where wild xoconostle plants (O. joconostle cv. Burro) grow and develop, the fruits presented increases in the quality parameters in weight of whole fruit and pulp, longitudinal and equatorial diameter, °Brix, pH, ash, fiber, protein, K+, Na+, Ca2+ y Mg2+ .The xoconostle fruits of wild plants of Chapantongo O. joconostle showed statistically significant differences between sites and quality parameters concerning to O. matudae cv. Cuaresmero. Xoconostle fruits can be considered in the diet as a nutraceutical food alternative. It can also be used as a natural ingredient in food, food products, and by-products.
土壤的物理和化学参数对农业植物的生理发育起决定性作用。在一些被称为xoconostle的仙人掌品种中,果实的物理和化学质量对它们生长的土壤的影响是未知的。本研究的目的是测定两种软果(Opuntia matudae cv.)的理化品质。Cuaresmero和O. joconconstle等。在墨西哥伊达尔戈的梅兹奎塔尔山谷种植和野生的驴。采用5次重复试验,对墨西哥伊达尔戈州种植和野生的松松果(O. matudae)和松松果(O. joconostle)土壤和果实的理化特性进行了分析。数据用JMP V8程序处理,用均数±标准差表示。果实均数之间的差异采用方差分析(ANOVA)处理,显著性水平为P?0.001。均数间差异采用Tukey检验,显著性水平为(P?0.05)。土壤理化性质表现为浅棕色(10YR 6/3),孔隙度高,pH值偏酸性,可交换离子含量高,其中野生松草属植物(O. joconostle cv。果实的质量参数(全果质量、果肉质量、纵向直径和赤道直径、白度、pH值、灰分、纤维、蛋白质、K+、Na+、Ca2+、y Mg2+)均呈显著性增加。Cuaresmero。在日常饮食中,软果可被视为一种营养食品替代品。它也可以用作食品、食品产品和副产品的天然成分。
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引用次数: 1
The association between morphological characteristics and yield in forage cactus clones varies according to water regimes: a principal component analysis 在饲料仙人掌无性系的形态特征和产量之间的关系根据水制度的变化:一个主成分分析
IF 0.9 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2022-06-11 DOI: 10.56890/jpacd.v24i.475
G. D. N. Araújo Júnior, L. S. Souza, A. M. D. R. F. Jardim, C. P. Alves, Méry Cristina de Sá Assis, José Oscar da Rocha Silva, C. A. A. Souza, A. G. Pinheiro, A. C. D. Oliveira, T. G. F. Silva
Forage cactus species exhibit distinct morphological characteristics, which have a relationship to yield and can be affected by different water conditions. The objective was to analyze, with the principal components analysis (PCA), the association between morphological and productivity variables in forage cactus clones in different water regimes. We cultivated, in Serra Talhada, Brazilian semiarid, the Miúda (MIU), Orelha de Elefante Mexicana (OEM) and IPA Sertânia (IPA) clones under four water regimes based on crop evapotranspiration – ETc (0%, 40%, 80% and 120% ETc). Between the years 2016 and 2018, morphological and crop productivity data were obtained. Two main components explained >82% of the total variability of the data, and the relationship between morphological and production variables depended on the clone and the water regime. The production of fresh and dry matter was more influenced by the morphological characteristics in the MIU and OEM clones. The IPA clone showed no correlation between the variables analyzed, regardless of the water regime. PCA can be used to understand the growth dynamics of plants and to identify the water conditions that act in the production of cactus clones, favoring better management in the cultivation of culture.
牧草仙人掌具有明显的形态特征,与产量有关,并受不同水分条件的影响。采用主成分分析法(PCA)分析了不同水分条件下饲草仙人掌无性系的形态和生产力变量之间的关系。我们在巴西半干旱地区Serra Talhada种植了Miúda(MIU)、Orelha de Elefante Mexicana(OEM)和IPA Sertânia(IPA)无性系,其水分状况基于作物蒸发蒸腾量(0%、40%、80%和120%ETc)。2016年至2018年间,获得了形态和作物生产力数据。两个主要成分解释了数据总变异性的>82%,形态和生产变量之间的关系取决于克隆和水情。新鲜和干物质的生产更多地受到MIU和OEM克隆的形态特征的影响。IPA克隆在所分析的变量之间没有显示出相关性,而与水分状况无关。PCA可用于了解植物的生长动态,并确定仙人掌无性系生产中的水分条件,有利于在培养中进行更好的管理。
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引用次数: 0
The inclusion of cactus pear changes the fermentation process, chemical composition and aerobic stability of arboreal cotton silages 仙人掌梨的加入改变了木棉青贮的发酵过程、化学成分和有氧稳定性
IF 0.9 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2022-04-29 DOI: 10.56890/jpacd.v24i.435
Daniel Anderson De Souza Melo, Ana Clara Silva Pinheiro Leite, Raquel da Silva Lima, Jessica Maria da Conceição da Silva Rodrigues, Cleyton de Almeida Araújo, Diego de Sousa Cunha, Claudenilde de Jesus Pinheiro Costa, Moema Kelly Nogueira de Sá, André Luíz Rodrigues Magãlhaes, Fleming Sena Campos
The objective was to evaluate the fermentation process, nutritional quality, and aerobic stability of mixed silages of arboreal cotton and cactus pear. This was a completely randomized design, with five levels of inclusion of cactus pear (0, 15, 30, 45, and 60%) in the arboreal cotton silage and four replications per treatment, totaling 20 experimental units. Data were submitted to analysis of variance and regression at a 5% probability level for type I error. The inclusion of cactus pear resulted in increased losses from gases (P<0.001), effluents (P<0.001), buffering capacity (P<0.001), aerobic stability (P = 0.010), and pH upward trend (P<0.001). There was a decreasing linear effect on dry matter recovery (P<0.001), pH (P<0.001), ammonia nitrogen (P <0.001), the maximum difference in the temperature of the silage in relation to the environment (P = 0.005) and maximum pH (P<0.001) of silages. The inclusion of cactus pear affected the maximum temperature (P<0.001) of silages in a quadratic way. There was a decreasing linear effect of dry matter (P<0.001), ether extract (P<0.001), Organic matter (P<0,001), crude protein (P = 0,002) and neutral detergent fiber corrected for ash and protein (P = 0.030). The use of cactus pear promoted an increasing linear effect for the levels of mineral matter (MM) (P<0.001), corresponding to an increase of 0,271%, for each 1% inclusion of cactus pear. The increase in the proportions of cactus pear in the silages promoted an increasing linear effect for mineral matter (P<0,001). The inclusion of cactus pear by up to 60% in arboreal cotton silages alters the fermentation profile with increased fermentation losses and nutritional reduction. However, it still presents characteristics of good quality silages.
目的是评价树栖棉和仙人掌梨混合青贮饲料的发酵过程、营养品质和好氧稳定性。这是一个完全随机的设计,在树栖棉花青贮饲料中加入五个水平的仙人掌梨(0、15、30、45和60%),每个处理重复四次,总共20个实验单位。数据以5%的概率水平提交给I型误差的方差分析和回归分析。仙人掌梨的加入增加了气体损失(P<0.001)、废水损失(P>0.001)、缓冲能力损失(P=0.001)、需氧稳定性损失(P=0.010)和pH值上升趋势(P<0.01),青贮饲料的温度与环境的最大差异(P=0.005)和青贮饲料的最大pH(P<0.001)。仙人掌梨对青贮饲料最高温度的影响呈二次型(P<0.001)。干物质(P<0.001)、乙醚提取物(P<001)、有机物(P<0001)、粗蛋白(P=0002)和中性洗涤剂纤维对灰分和蛋白质的校正作用呈线性下降(P=0.030)。仙人掌梨的使用促进了矿物质(MM)水平的线性上升(P<0.01),对应于仙人掌梨每包含1%,增加0271%。青贮饲料中仙人掌梨比例的增加促进了矿物质的线性效应增加(P<0001)。仙人掌梨在树栖棉花青贮饲料中的含量高达60%,改变了发酵特性,增加了发酵损失,减少了营养。然而,它仍然呈现出优质硅烷的特点。
{"title":"The inclusion of cactus pear changes the fermentation process, chemical composition and aerobic stability of arboreal cotton silages","authors":"Daniel Anderson De Souza Melo, Ana Clara Silva Pinheiro Leite, Raquel da Silva Lima, Jessica Maria da Conceição da Silva Rodrigues, Cleyton de Almeida Araújo, Diego de Sousa Cunha, Claudenilde de Jesus Pinheiro Costa, Moema Kelly Nogueira de Sá, André Luíz Rodrigues Magãlhaes, Fleming Sena Campos","doi":"10.56890/jpacd.v24i.435","DOIUrl":"https://doi.org/10.56890/jpacd.v24i.435","url":null,"abstract":"The objective was to evaluate the fermentation process, nutritional quality, and aerobic stability of mixed silages of arboreal cotton and cactus pear. This was a completely randomized design, with five levels of inclusion of cactus pear (0, 15, 30, 45, and 60%) in the arboreal cotton silage and four replications per treatment, totaling 20 experimental units. Data were submitted to analysis of variance and regression at a 5% probability level for type I error. The inclusion of cactus pear resulted in increased losses from gases (P<0.001), effluents (P<0.001), buffering capacity (P<0.001), aerobic stability (P = 0.010), and pH upward trend (P<0.001). There was a decreasing linear effect on dry matter recovery (P<0.001), pH (P<0.001), ammonia nitrogen (P <0.001), the maximum difference in the temperature of the silage in relation to the environment (P = 0.005) and maximum pH (P<0.001) of silages. The inclusion of cactus pear affected the maximum temperature (P<0.001) of silages in a quadratic way. There was a decreasing linear effect of dry matter (P<0.001), ether extract (P<0.001), Organic matter (P<0,001), crude protein (P = 0,002) and neutral detergent fiber corrected for ash and protein (P = 0.030). The use of cactus pear promoted an increasing linear effect for the levels of mineral matter (MM) (P<0.001), corresponding to an increase of 0,271%, for each 1% inclusion of cactus pear. The increase in the proportions of cactus pear in the silages promoted an increasing linear effect for mineral matter (P<0,001). The inclusion of cactus pear by up to 60% in arboreal cotton silages alters the fermentation profile with increased fermentation losses and nutritional reduction. However, it still presents characteristics of good quality silages.","PeriodicalId":54361,"journal":{"name":"Journal of the Professional Association for Cactus Development","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2022-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48313989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cultural perception as a primary factor in the market for Red Sour Pitaya (Stenocereus gummosus) in the region of La Paz, Baja California Sur 文化观念是影响下加利福尼亚南部拉巴斯地区红酸火龙果市场的主要因素
IF 0.9 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2022-03-23 DOI: 10.56890/jpacd.v24i.489
Manuel Arturo Coronado-García, Jorge Arnoldo Villegas Espinoza, Luis Carlos Amador Betancourt, Sergio Ramón Rosetti-López, I. Rojas-Rodríguez
The study includes the analysis of cultural, commercial, and safety factors that affect the potential of the trade of the red sour pitaya (Stenocereus gummosus), as well as its characterization, consumer patterns, consumption channels, and the relevance, identity, and cultural value of the fruit in the Municipality of La Paz, Baja California Sur, Mexico. The investigation has the objective of understanding the perception of the product in local knowledge, which is identified in the study area, where the wild fruit takes an added value in informal commercialization and is amalgamated with the local culture. For the analysis and data collection, a simple random sampling was conducted, on 227 economically active people with purchasing power. The method of applying the instrument was digital, limiting face-to-face contact with people due to the current Covid 19 pandemic. The exercise characterizes a population with mostly university studies, which consumes the product in a traditional way, which reflected a greater appreciation of the pitaya fruit, by older people.
该研究包括对影响墨西哥下加利福尼亚南部拉巴斯市红酸火龙果(Stenocereus gummosus)贸易潜力的文化、商业和安全因素的分析,以及其特性、消费者模式、消费渠道以及水果的相关性、身份和文化价值。调查的目的是了解产品在当地知识中的感知,这是在研究区域确定的,在那里野果在非正式商业化中具有附加价值,并与当地文化融为一体。为了分析和收集数据,我们对227名具有购买力的经济活动人士进行了简单的随机抽样。使用仪器的方法是数字化的,由于目前的Covid - 19大流行,限制了与人的面对面接触。这种做法的特点是,大多数人都是大学毕业生,他们以传统的方式消费火龙果,这反映出老年人对火龙果的喜爱程度更高。
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引用次数: 0
Anaerobic co-digestion of Opuntia ficus-indica (L.) Mill. cladode with cow manure: Effect of different cultivars on biochemical methane potential 无花果机会菌厌氧共消化的研究轧机。牛粪加枝线虫:不同品种对生化甲烷势的影响
IF 0.9 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2022-03-23 DOI: 10.56890/jpacd.v24i.453
T. Espinosa-Solares, J. E. Aguilar-Toalá, Christell Barrales-Fernández, José Carlos Meneses-Reyes, C. GALLEGOS-VÁZQUEZ, G. Hernández-Eugenio
Nopal (Opuntia ficus-indica (L.) Mill.) cladode has garnered great interest recently in the area of agro-energy as emerging biomass due to its sustainable production. The objective of this study was to compare the biochemical methane potential of different nopal cultivars in co-digestion with cow manure. For this purpose, two different nopal cladodes: cow manure proportions (75:25 and 82:18) and three different cultivars (Atlixco, Copena V1, and Milpa Alta) were evaluated. The results indicated that the treatments with higher biochemical methane potential (mL CH4 g-1 VSfed) were Milpa Alta 75:25 (71.4), Copena V1 75:25 (66.5), Milpa Alta 82:18 (64.6), and Copena V1 82:18 (59.0), which showed no statistical difference (P>0.05) between them, whereas the Atlixco treatments (75:25 and 82:18) had the lowest (P<0.05) values (52.8 and 41.5, respectively). The results suggest that the cow manure proportion and nopal cultivar used in a co-digestion system may influence its biochemical methane potential.
无花果(Opuntia ficus-indica)由于其可持续性生产,最近在农业能源领域引起了人们的极大兴趣。本研究的目的是比较不同品种野菜与牛粪共消化的生化甲烷势。为此,对两种不同品种(牛粪比例分别为75:25和82:18)和3个不同品种(Atlixco、Copena V1和Milpa Alta)进行了评价。结果表明:生化甲烷势(mL CH4 g-1 VSfed)较高的处理为Milpa Alta 75:25(71.4)、Copena V1 75:25(66.5)、Milpa Alta 82:18(64.6)和Copena V1 82:18(59.0),差异无统计学意义(P<0.05),而Atlixco处理(75:25和82:18)的生化甲烷势最低(分别为52.8和41.5)。结果表明,牛粪比例和共消化系统中使用的nopal品种可能影响其生化甲烷势。
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引用次数: 0
Seed germination of Opuntia dejecta, a non-desert cactus native to Central America 仙人掌的种子发芽,仙人掌是一种原产于中美洲的非沙漠仙人掌
IF 0.9 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2022-02-21 DOI: 10.56890/jpacd.v24i.488
Rubelsi Matus, Y. Perroni, Miguel Virgen, J. Flores, José Antonio Miranda-Jácome
Opuntia species from arid and semiarid environments have dormant seeds. The objective of this study was to evaluate how the soil influences seed germination of the cactus O. dejecta. We hypothesized that O. dejecta seeds in the basaltic rocky soil will show lower seed germination than seeds in a site with alluvial soil from the coastal plain. An experiment of partial reciprocal transplant was performed, placing seeds from basaltic soil (San Ignacio population) in pots containing alluvial soil from Puente Nacional population and in slabs from basaltic soil, and placing them on two greenhouses, in San Ignacio population and Puente Nacional population. We found that seed germination (%) was lower in San Ignacio site (with basaltic soil) than in Puente Nacional site (with alluvial soil), and that alluvial soil showed higher seed germination than basaltic soil, as well as higher seed germination in Puente Nacional than in San Ignacio. The results suggest that seed dormancy for the population situated in the rocky condition is a trait locally adapted, and the basaltic soil can be considered as a selection factor.
来自干旱和半干旱环境的仙人掌有休眠的种子。本研究的目的是评估土壤对仙人掌种子发芽的影响。我们假设,与海岸平原冲积土中的种子相比,玄武岩岩石土壤中的粪便O.种子发芽率较低。进行了部分互惠移植实验,将玄武岩土壤(圣伊格纳西奥种群)的种子放入盛有Puente Nacional种群冲积土的花盆中,并将其放入玄武岩土壤的平板中,然后将其放置在圣伊格纳乔种群和Puente纳cional群落的两个温室中。我们发现,San Ignacio场地(含玄武岩土壤)的种子发芽率(%)低于Puente Nacional场地(含冲积土),冲积土的种子发芽度高于玄武岩土壤,Puente纳cional的种子发芽力也高于San Ignacio。结果表明,岩石条件下群体的种子休眠是一种局部适应性特征,玄武岩土壤可以作为一个选择因素。
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引用次数: 1
Establishment of callus and cell suspension cultures of Ariocarpus retusus (Scheidweiler) 羊蹄木愈伤组织的建立及细胞悬浮培养
IF 0.9 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2022-02-16 DOI: 10.56890/jpacd.v24i.454
I. Fiedler, F. Santacruz-Ruvalcaba, L. Portillo
Ariocarpus retusus is an endemic species of Mexico subject to special protection by the Mexican government due to its vulnerability to habitat disturbance. Previous studies have reported secondary metabolites in A. retusus with interesting biological activity. However, the use of these secondary metabolites has limitations. The aim of this study was to establish protocols for obtaining friable callus and cell suspension cultures that allow the subsequent development of strategies for obtaining secondary metabolites. Seedlings were obtained from seeds which were exposed to different concentrations of auxins and cytokinins to induce callogenesis. The callus generated was subcultured in Murashige and Skoog (MS) medium with modifications in the content of ammonium nitrate and potassium nitrate, and incubated in different photoperiod conditions (16:8 h light: dark and continuous darkness). Likewise, cell suspensions were established and characterized by friable callus. The MS medium supplemented with 2 mg/L 2,4-D, 2 mg/L BAP, and 3 mg/L KIN allowed the development of vigorous callus. Callus friability was enhanced by decreasing ammonium nitrate (410 mg/L) and increasing potassium nitrate (2850 mg/L). The absence of light during incubation induced friable callus. The addition of myo-inositol (3 mg/L) significantly (p = 0.000) influenced the increase in biomass of cell suspensions.
Ariocarpus reusus是墨西哥特有物种,因其易受生境干扰而受到墨西哥政府的特别保护。以往的研究报道了兔耳螨的次生代谢物具有有趣的生物活性。然而,这些次生代谢物的使用有局限性。本研究的目的是建立获得易碎愈伤组织和细胞悬浮培养的方案,以便随后开发获得次生代谢物的策略。将种子分别置于不同浓度的生长素和细胞分裂素中,诱导其形成胼胝质。将产生的愈伤组织在修改硝酸铵和硝酸钾含量的Murashige和Skoog (MS)培养基中传代培养,并在不同的光周期条件下(16:8 h光照、黑暗和连续黑暗)培养。同样,细胞悬浮液的建立和特征是易碎的愈伤组织。在MS培养基中添加2mg /L 2,4- d、2mg /L BAP和3mg /L KIN,愈伤组织发育旺盛。减少硝酸铵(410 mg/L)和增加硝酸钾(2850 mg/L)可增强愈伤组织的脆性。培养过程中缺乏光照诱导出易碎的愈伤组织。添加肌醇(3 mg/L)显著影响细胞悬浮液生物量的增加(p = 0.000)。
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引用次数: 0
期刊
Journal of the Professional Association for Cactus Development
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