Steel-aluminum transition joints are commonly produced through explosive welding and friction welding techniques, serving to link aluminum pressure vessels with steel pipes within cold boxes in air separation unit. This study investigates the impact of post-weld heat treatment (PWHT) on the microstructure and mechanical properties of steel-aluminum transition joints created via induction roll welded (IRW), utilizing A283GRC steel and 5052 aluminum alloy as substrates. The findings suggest that the types of intermetallic compounds (IMCs) in IRW joints remain unchanged before and after heat treatment. The thickness of interfacial IMCs increases with higher annealing temperature and longer annealing time, with a faster growth rate at higher annealing temperatures. After PWHT, the grain size near the interface of the joint on the steel side decreased, with the most significant decrease observed when annealed at 300 °C for 2 h. While cracks in the interface zone gradually diminish or disappear with PWHT, excessive heat treatment temperature or duration may result in new transverse cracks. At an annealing temperature of 200 °C, there is limited growth range for IMCs and noticeable repair effect on cracks within the interface region. When annealed at 300 °C and 400 °C, there is a decrease in joint hardness compared to before heat treatment levels, and this decreasing rate accelerates with higher annealing temperature and longer duration. Following annealing at 300 °C for 2 h, the shear strength of the sample reached 70.52 MPa, which is 32 % higher than that before heat treatment. Overall findings suggest that the annealing temperature exerts a more pronounced impact on the mechanical properties of joints in comparison to the annealing duration across the investigated time and temperature ranges. The fracture mode exhibited by the samples before and after heat treatment in IRW is characterized by a mixed fracture mode. However, the predominant fracture mode observed without heat treatment is brittle fracture, whereas after heat treatment, ductile fracture becomes the primary mode of fracture.