Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology最新文献

{"title":"Temporal changes in PTTH/Egf signaling and ERK target gene expressions during chilling-induced diapause termination in Bombyx mori eggs","authors":"Shi-Hong Gu","doi":"10.1016/j.cbpa.2025.111884","DOIUrl":"10.1016/j.cbpa.2025.111884","url":null,"abstract":"<div><div>Activation of extracellular signal-regulated kinase (ERK) signaling is related to chilling-induced diapause termination in <em>Bombyx mori</em> eggs. However, the changes in expressions of upstream signaling and downstream target genes are not very clear. In the present study, I investigated temporal changes in gene expressions of the prothoracicotropic hormone (PTTH) and epidermal growth factor (Egf) signaling pathways, which are the major stimulators of ERK signaling in insect systems, during a long chilling period. My results showed that when chilling was conducted from 2 days post-oviposition, expressions of PTTH/Egf signaling pathway genes were maintained at relatively higher levels, compared to those kept at 25 °C. In eggs exposed to 5 °C from 15 days post-oviposition, expression of the <em>PTTH</em> gene had dramatically increased by day 45 after chilling. Egf signaling pathway genes, including <em>spitz</em> (<em>spi</em>), <em>vein</em> (<em>vn</em>), <em>rhomboid</em> (<em>rho</em>), and <em>Egf receptor</em> (<em>Egfr</em>), showed gene-specific increasing patterns during the chilling period. I further showed that expression of the <em>pointed</em> (an ERK target) gene was maintained at a relatively higher when chilling was conducted from 2 days post-oviposition. Expression of the <em>pointed</em> gene had dramatically increased by day 45 in eggs chilled beginning after 15 days post-oviposition. Additionally, dechorionation increased <em>pointed</em> gene expression. LY294002 (a specific phosphatidylinositol 3-kinase (PI3K) inhibitor) and U0126 (an ERK inhibitor) inhibited <em>pointed</em> gene expression in dechorionated eggs, indicating that both PI3K and ERK are involved in increased <em>pointed</em> gene expression. When eggs that had been kept at 5 °C for 30 days were transferred to 25 °C, expression levels of <em>PTTH</em> and <em>pointed</em> genes decreased after transfer, clearly indicating that continuous chilling for 45 days is necessary to sustain the higher expressions of these genes. To the best of my knowledge, this study presents the first comprehensive analysis of the transcriptional regulation of both upstream and downstream genes in the ERK signaling pathway during a chilling period, offering valuable insights into the mechanisms underlying chilling-induced diapause termination.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111884"},"PeriodicalIF":2.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic changes associated with live transport stress of Haliotis rubra abalone","authors":"Nguyen Van Thao ,&nbsp;Jaime McAllister ,&nbsp;Andrea C. Alfaro ,&nbsp;Nha T. Ngo ,&nbsp;Craig Mundy","doi":"10.1016/j.cbpa.2025.111883","DOIUrl":"10.1016/j.cbpa.2025.111883","url":null,"abstract":"<div><div>Transport stress significantly influences the well-being and survival of abalone, yet the underlying metabolic changes and physiological responses associated with this stress remain poorly understood. To gain comprehensive insights into the metabolic changes and physiological responses of abalone (<em>Haliotis rubra</em>) under transport stress, we conducted a targeted liquid chromatography–mass spectrometry (LC–MS) metabolomics investigation on haemolymph and gill samples collected at different time points, including post-harvesting (pre-transport), post-transport and post-immersion (water holding period) and post-live exporting transport. The results revealed 143 and 141 metabolites that were significantly different among sampling times in haemolymph and gill, respectively. Notably, most of the metabolite differences occurred between the post-transport and post-immersion times, which indicate the strong impacts of transport stress on abalone metabolism. Interestingly, certain metabolites, such as lactic acid, succinic acid, L-hydroxyglutaric acid, uric acid, and myo-inositol, showed time-dependent increases during transport, suggesting their potential as stress biomarkers in abalone. Moreover, abalone that were acclimatized in holding tanks exhibited lesser metabolic changes compared to non-acclimatized, despite both groups being transported with oxygen supply. This highlights the significance of acclimatization and oxygen supply in reducing stress for abalone during transport. Enrichment analysis on abalone samples at 24 h post-transport compared to 96 h post-immersion identified 12 significantly impacted pathways in haemolymph and 34 pathways in gill tissues, indicating a range of metabolic disturbances in transported abalone, such as energy-related pathways, amino acid metabolisms, carbohydrate metabolisms, vitamin metabolisms, oxidative stress, and others. These results offer valuable insights into the physiological responses of abalone to transport stress, guiding improved transport practices to ensure good quality of products arriving at their market destinations.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111883"},"PeriodicalIF":2.1,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144210317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacology reveals neuropeptide Y as a novel differential regulator of adrenal steroids in the globally invasive red-eared slider turtle (Trachemys scripta elegans)","authors":"Yoke Tassent,&nbsp;H. Bobby Fokidis","doi":"10.1016/j.cbpa.2025.111882","DOIUrl":"10.1016/j.cbpa.2025.111882","url":null,"abstract":"<div><div>The regulation of adrenal steroid secretion during acute stress has been poorly explored in reptiles. Using pharmacological manipulations of adrenocorticotropic hormone (ACTH), dexamethasone (DEX), neuropeptide Y (NPY), and NPY receptor antagonists, we examined glucocorticoid and DHEA responses to identify pathways potentially linking stress adaptation and energy homeostasis in the red-eared slider (<em>Trachemys scripta elegans</em>). Acute handling stress increased corticosterone concentrations as did injection with ACTH, but NPY and receptor antagonist treatments prevented this endogenous increase in corticosterone. In contrast, cortisol, and DHEA were not impacted by handling stress or ACTH, but both steroids increased in response to injection with NPY and with an established Y2 receptor antagonist. Together these data reveal the possibility that both ACTH and NPY act to regulate adrenal steroid synthesis, through a complicated and currently poorly understood mechanism. As red-eared sliders are highly invasive on a global scale, these insights can enhance our understanding of reptilian stress physiology to inform management strategies as well as bridge the gap between fundamental biology and applied conservation efforts, demonstrating the broader value of integrative research in addressing ecological and biomedical challenges.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111882"},"PeriodicalIF":2.1,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dealing with the heat: Assessing heat stress in an Arctic seabird using 3D-printed thermal models","authors":"Fred Tremblay ,&nbsp;Emily S. Choy ,&nbsp;David A. Fifield ,&nbsp;Glenn J. Tattersall ,&nbsp;François Vézina ,&nbsp;Ryan O'Connor ,&nbsp;Oliver P. Love ,&nbsp;Grant H. Gilchrist ,&nbsp;Kyle H. Elliott","doi":"10.1016/j.cbpa.2025.111880","DOIUrl":"10.1016/j.cbpa.2025.111880","url":null,"abstract":"<div><div>The Arctic is warming at four times the global average rate and most studies have focused on the indirect (e.g., changes in food web) rather than the direct effects of climate change. However, as Arctic animals often have low capacity to dissipate heat, the direct effect of warming could impact them significantly (heat stress). To study heat stress, biophysical models have been used in many species to estimate operative temperature (Te, integrated temperature of the thermal environment experienced by an individual). Here, we developed biophysical models of an Arctic seabird, the thick-billed murre (<em>Uria lomvia</em>). We demonstrated that 3D-printed painted models perform similarly to the more traditionally used feather-covered models. We deployed our models on Coats Island, Nunavut, Canada to study heat stress, which occurs in murres when operative temperature is above 21.2 °C (the temperature at which evaporative water loss (EWL) rates increase to maintain a constant body temperatures). Murre operative temperatures ranged from 5.5 °C to 46.5 °C despite ambient temperatures never exceeding 24.7 °C (range: 3.4–24.7 °C), and murres experienced heat stress on 61 % of the days during the breeding season (range: 24–85 %). Using known equations of EWL as a function of temperature, we estimated that murres lost 3.79 % to 4.61 % of their body mass in water daily. Our study confirms the physiological challenges faced by Arctic seabirds during the breeding season, while also demonstrating the value of biophysical models as non-invasive tools to study the effects of heat stress on seabirds.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"306 ","pages":"Article 111880"},"PeriodicalIF":2.1,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144113110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic responses to thermal ramping in two endemic eurybathic amphipods of the genus Ommatogammarus from ancient Lake Baikal","authors":"Yulia Shirokova ,&nbsp;Ekaterina Telnes (Madyarova) ,&nbsp;Andrey Mutin ,&nbsp;Yaroslav Rzhechitskiy ,&nbsp;Zhanna Shatilina ,&nbsp;Inna Sokolova ,&nbsp;Maxim Timofeyev","doi":"10.1016/j.cbpa.2025.111881","DOIUrl":"10.1016/j.cbpa.2025.111881","url":null,"abstract":"<div><div>Lake Baikal harbors freshwater profundal amphipods, including the eurybathic species <em>Ommatogammarus flavus</em> and <em>O. albinus</em>. <em>O. flavus</em> occupies shallower habitats, including the littoral zone, which is subject to greater temperature variability. Consequently, <em>O. flavus</em> may demonstrate a higher degree of tolerance to temperature fluctuations in comparison with <em>O. albinus</em>. We compared the metabolic responses of these two amphipod species to thermal ramping. Specimens were collected from a range of depths and acclimated to a temperature of 4 °C. They were then subjected to controlled temperature up- and downshifts to assess the parameters of tissue energy status, antioxidant enzyme activity, and survival. <em>O. flavus</em> showed a higher degree of tolerance to warming than the deeper-dwelling <em>O. albinus</em>, while both species were resilient to temperature decreases. The depth of sampling had a minor effect on glycogen levels in <em>O. flavus</em> as well as catalase and glutathione S-transferase activity in <em>O. albinus</em> but had no effect on survival during experimental warming. Glucose level was shown to be the most sensitive biochemical marker to temperature variations, indicating that it could be used as a stress indicator for Baikal deep-water amphipods. This finding might provide insight into their adaptability to ambient temperature fluctuations.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"306 ","pages":"Article 111881"},"PeriodicalIF":2.1,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144087090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Transient Receptor Potential Cation Channel subfamily Vanilloid 4 (TRPV4) in chub mackerel: Role in low-salinity acclimation and reproductive implications","authors":"Kyle Dominic Barnuevo ,&nbsp;Nguyen The Vuong ,&nbsp;Sipra Mohapatra ,&nbsp;Oga Sato ,&nbsp;Hironori Katoh ,&nbsp;Takehiko Itoh ,&nbsp;Naoki Nagano ,&nbsp;Michiya Matsuyama ,&nbsp;Tapas Chakraborty ,&nbsp;Kohei Ohta","doi":"10.1016/j.cbpa.2025.111879","DOIUrl":"10.1016/j.cbpa.2025.111879","url":null,"abstract":"<div><div>Salinity plays a crucial role in fish physiology, including osmoregulation, metabolism, growth, reproduction, and disease resistance. In this study, we characterized the Transient Receptor Potential Cation Channel Subfamily Vanilloid 4 (<em>trpv4</em>) gene and investigated the effects of low-salinity conditions on its expression in various tissues of chub mackerel (<em>Scomber japonicus</em>), an economically important aquaculture species. The <em>trpv4</em> open reading frame (ORF) consists of 2616 nucleotides and encodes an 872-amino acid protein. It shares up to 94.01 % phylogenetic similarity with <em>Thunnus maccoyii</em> and possesses 6 transmembrane domains highly conserved with human TRPV4. <em>Trpv4</em> was widely distributed across most organs and showed significant sex-biased expression. <em>In situ</em> hybridization data showed that <em>trpv4</em> was explicitly expressed in various parts of the pituitary, from <em>pars intermedia</em> (PI) to <em>rostral pars distalis</em> (RPD) where it colocalized with <em>prolactin 1</em> (<em>prl1</em>) in RPD. Furthermore, exposure to decreasing salinity from 35 ppt to 10 ppt demonstrated a significant upregulation of <em>trpv4</em> transcription in the pituitary, gill, skin, and gonad under low-salinity conditions. Although <em>prl1</em> was upregulated, <em>follicle-stimulating hormone beta subunit</em> (<em>fshb</em>) expression was decreased in the pituitaries of low-salinity groups. These findings suggest that <em>trpv4</em> is regulated by salinity in chub mackerel and may play a role in reproductive responses to salinity changes. Overall, these results highlight the characterization of <em>trpv4</em> in marine fish and its potential roles in osmoregulation, as well as in transmitting low-salinity signals to reproduction-related organs. The involvement of the brain–pituitary–gonad axis in this response warrants further investigation.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"306 ","pages":"Article 111879"},"PeriodicalIF":2.1,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144087088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal regulation and PIP2 dependence of inwardly rectifying potassium channels (Kir) in retinal horizontal cells of goldfish (Carassius auratus)","authors":"Shilo L. Rousseau ,&nbsp;Michael G. Jonz","doi":"10.1016/j.cbpa.2025.111877","DOIUrl":"10.1016/j.cbpa.2025.111877","url":null,"abstract":"<div><div>Many species face extreme changes in their environment throughout the year, during which time they must modify their neuronal activity to survive. Horizontal cells (HCs) are interneurons in the goldfish (<em>Carassius auratus</em>) retina that demonstrate seasonal changes in membrane structure and excitability. Excitability in neurons is strongly influenced by inwardly rectifying potassium channels (K_ir), suggesting that K_ir in HCs may be a candidate for modulating seasonal changes. K_ir channel function depends on the plasma membrane phospholipid, PIP₂, to which it is bound. However, it remains unclear whether the PIP₂-K_ir channel interaction contributes to regulation of K_ir in goldfish HCs. Using perforated patch voltage-clamp recording, we identified K_ir current by inhibition with cesium (Cs⁺) and compared peak inward K_ir current density in goldfish HCs isolated during the summer and winter months. Significantly more current was inhibited by Cs⁺ in winter HCs, suggesting more open K_ir channels. To examine the role of PIP₂ in regulating K_ir, tamoxifen or activation of phospholipase C by m-3M3FBS were used to disrupt the PIP₂-K_ir channel interaction and subsequently reduced K_ir current. Spermine, through strengthening the PIP₂-K_ir interaction, reduced the inhibitory effects of tamoxifen. Additionally, in current-clamp recordings, HCs treated with m-3M3FBS displayed a depolarized membrane potential (V_m) and a decrease in spontaneous Ca²⁺-based action potentials. We demonstrate that K_ir activity is seasonally regulated in goldfish HCs and that K_ir is dependent upon PIP₂. Our results suggest PIP₂ as a potential target for coordinating seasonal changes in the activity of neurons in the goldfish retina.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"306 ","pages":"Article 111877"},"PeriodicalIF":2.1,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144060367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anorexigenic and anxiogenic effects of the plasticiser DEHP (di-2-ethylhexyl phthalate) in goldfish: Involvement of PPAR signalling and feeding-related neuropeptides","authors":"Lisbeth Herrera-Castillo,&nbsp;Claudia Hernández-Villasevil,&nbsp;André Barany,&nbsp;Miguel Gómez-Boronat,&nbsp;Esther Isorna,&nbsp;Nuria de Pedro","doi":"10.1016/j.cbpa.2025.111878","DOIUrl":"10.1016/j.cbpa.2025.111878","url":null,"abstract":"<div><div>Di-2-ethylhexyl phthalate (DEHP), a widely used plasticiser, is a pervasive environmental contaminant with potential detrimental effects on aquatic organisms. The objective of this study was to provide an integrative analysis of how DEHP alters energy balance, temporal homeostasis and fish welfare — interrelated aspects critical to animal survival — to address critical gaps in our understanding of its toxicological effects. Goldfish (<em>Carassius auratus</em>) were chronically (14 days) treated with DEHP. Energy balance was assessed through locomotor activity, metabolic rate, feed intake, and growth indices. Daily of locomotor and metabolic rate rhythms were examined to explore potential circadian disruptions. Anxiety-like behaviours were also examined to assess welfare. DEHP decreased feed intake and food-anticipatory activity (FAA), suggesting an anorexigenic effect, which may have been mediated by increased expression of anorexigenic genes in the hypothalamus and liver, along with decreased expression of orexigenic <em>npy</em> (neuropeptide Y) gene in the hypothalamus. Growth parameters remained unchanged, probably due to compensatory reductions in energy expenditure, as indicated by decreased locomotor activity and metabolic rate. Daily rhythms in these two parameters were preserved, suggesting no disruption in temporal homeostasis. DEHP increased hepatic expression of peroxisome proliferator-activated receptor (PPAR)-related genes, suggesting that PPARs activation is a potential mode of action for DEHP in fish. Anxiety levels were elevated, as evidenced by increased thigmotaxis and scototaxis in behavioural tests, which may be mediated by changes in hypothalamic neuropeptides. These findings highlight the adverse effects of DEHP on energy regulation and animal welfare, providing novel insights into its broader physiological consequences in fish.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"306 ","pages":"Article 111878"},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complementary DNA (cDNA) cloning and expression analysis of selenium glutathione peroxidase (Se-GPx) and glutathione S-transferases (GST) 1 in red claw crayfish (Cherax quadricarinatus)","authors":"Jia-Han Liu ,&nbsp;Jun-Tao Li ,&nbsp;Yao-Peng Lu ,&nbsp;Pei-Hua Zheng ,&nbsp;Ze-Long Zhang ,&nbsp;Xiu-Xia Zhang ,&nbsp;Chi Xu ,&nbsp;Zi-Hang Yu ,&nbsp;Xiu-Ying Yang ,&nbsp;Hui Guo ,&nbsp;Jian-An Xian","doi":"10.1016/j.cbpa.2025.111876","DOIUrl":"10.1016/j.cbpa.2025.111876","url":null,"abstract":"<div><div>Selenium glutathione peroxidase (Se-GPx) and Glutathione S-transferases (GST) are crucial parts of cellular detoxification systems that protect cells from reactive oxygen species (ROS). In this study, cDNA sequences of CqGST1 and CqSe-GPx were cloned from red claw crayfish (<em>Cherax quadricarinatus</em>). The molecular masses of the predicted protein are 25.36 and 21.49 kDa, respectively. CqGST1 and CqSe-GPx have open reading frames (ORFs) of 657 bp and 570 bp and encode peptides of 218 and 189 amino acids, respectively. Sequence alignment showed that the amino acid sequence of CqSe-GPx identity with that of the <em>Exopalaemon carinicauda</em>, <em>Penaeus japonicus</em>, <em>Macrobrachium rosenbergii</em>, <em>Penaeus monodon</em>, <em>Litopenaeus vannamei</em>, <em>Macrobrachium nipponense</em> and <em>Scylla paramamosai</em>. The amino acid sequence of CqGST1 identity with <em>Procambarus clarkii</em>, <em>Stegastes partitus</em>, <em>Gavialis gangeticus</em>, <em>Alligator mississippiensis,</em> and L. <em>vannamei</em>. The qRT-PCR results showed that CqSe-GPx and CqGST1 were found in a variety of tissues, with the highest hemolymph expression and the weakest expression in muscle, eye stalk, and heart. The expression levels of CqSe-GPx and CqGST1 in the hepatopancreas and gills of crayfish were detected under ammonia-N and microcystins-LR stresses. The expression of CqSe-GPx was induced in the hepatopancreas of crayfish during MC-LR stress, and gill CqSe-GPx expression levels were induced at 6 and 12 h. Hepatopancreas and gill CqSe-GPx expression levels were significantly up-regulated during ammonia-N at 24 and 48 h, respectively. The CqGST1 expression levels were significantly elevated in the hepatopancreas and gill during MC-LR or ammonia-N stress. Under ammonia stress, hepatopancreas and gill GST expression levels of crayfish showed a decreasing and then increasing trend. Hepatopancreas and gill CqGST1 expression levels of crayfish were significantly up-regulated at 12, 24, and 48 h of ammonia-N stress. These results suggest that CqSe-GPx and CqGST1 play an important role in protecting <em>C. quadricarinatus</em> from ammonia-N and MC-LR stress and provide a theoretical basis for further study on the molecular mechanism of response to environmental stress on crayfish.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"306 ","pages":"Article 111876"},"PeriodicalIF":2.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acidification affects the early development of Colombian endemic fish Prochilodus magdalenae","authors":"Villamizar Natalia ,&nbsp;Guardiola Jose Luis ,&nbsp;Cruz-Botto Sara ,&nbsp;Rodríguez Hector Alejandro ,&nbsp;Reyes-Bermúdez Alejandro ,&nbsp;Noisette Fanny","doi":"10.1016/j.cbpa.2025.111875","DOIUrl":"10.1016/j.cbpa.2025.111875","url":null,"abstract":"<div><div>There is a significant knowledge gap regarding the acidification of freshwater ecosystems and its effects on biological systems. The bocachico (<em>Prochilodus magdalenae</em>), an endemic and migratory species vital to Colombia's inland fisheries, is currently classified as vulnerable. This study evaluated the impact of different pH levels (6.2, 7.2, and 7.6), previously recorded in the species' natural habitat, on its early development. Using an automated IKS Aquastar system, embryo incubation and larval maintenance were monitored from 0 to 5 days post-fertilization, assessing development, hatching, and survival at both organismal and transcriptional levels. Embryos exposed to pH 6.2 showed delayed development within 4 h post-fertilization, the lowest hatching rate (68.33 ± 3.13 %), and survival (23.88 ± 4.53 %), along with the highest incidence of malformations (37.80 ± 4.4 %). The pH 7.6 group also showed adverse effects, but to a lesser extent. Transcriptome analysis revealed a distinct molecular response in the pH 6.2 group, identifying 1214 differentially expressed genes related to early development, ossification, organ formation, sensory systems, and cellular processes. The findings indicate that pH fluctuations previously observed in the species' natural environment significantly affect <em>P. magdalenae</em> during early life stages, which raises serious concerns about the long-term viability of this endemic species and the sustainability of the artisanal fisheries that depend on it.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"306 ","pages":"Article 111875"},"PeriodicalIF":2.1,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}