Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology最新文献

{"title":"Abnormalities in trehalose homeostasis reduce autophagy, antioxidant and response to air exposure stress in Eriocheir sinensis","authors":"Anjun Xiang ,&nbsp;Jiayi Chen ,&nbsp;Chengyi Weng ,&nbsp;Chenxu Liu ,&nbsp;Jie Zhang ,&nbsp;Qing He ,&nbsp;Yongxu Cheng ,&nbsp;Xiaozhen Yang","doi":"10.1016/j.cbpa.2025.111898","DOIUrl":"10.1016/j.cbpa.2025.111898","url":null,"abstract":"<div><div>Trehalose, a key carbohydrate in crustaceans and insects, is important in enhancing stress resistance. However, its regulatory mechanisms and functions in Chinese mitten crabs (<em>Eriocheir sinensis</em>) remain unclear. This study was designed to explore the regulation mechanisms of trehalose anabolism by synthesizing dsRNA interference fragments to target trehalose-6-phosphate synthase (<em>TPS</em>) and trehalose hydrolase (<em>TREH</em>) genes, investigating the effects of endogenous trehalose anabolism, antioxidant function, and autophagy in crabs under air exposure stress (AES). First, dsRNAs were synthesized, and interference fragments (EGFP, dsTPS1, and dsTREH2) and the optimal interference duration (48 h) were screened by in vitro culture. Further in vivo experiments demonstrated that interference with <em>TPS</em> and <em>TREH</em> genes led to abnormal trehalose anabolism in crabs subjected to AES; following interference, the expression of <em>TPS</em> gene was significantly down-regulated, while that of trehalose transporters (<em>TRET</em>) genes was significantly up-regulated; in the hepatopancreas, the levels of trehalose and glucose (Glu) were notably increased, whereas their levels in the hemolymph were significantly decreased. Abnormalities in trehalose homeostasis led to down-regulated expression of autophagy genes (<em>ATG4B</em>, <em>ATG7</em>, <em>ATG13</em>, <em>Beclin1</em>) and its activation pathway, MAPK signaling pathway genes (<em>ERK</em>, <em>JNK</em>, <em>p38</em>); this was accompanied by a decrease in p38 protein level, which ultimately led to decreased antioxidant capacity, increased vacuolated cells, and disturbed cellular arrangement in hepatopancreatic tissues of crabs under AES; there was an up-regulation of the expression of genes such as catalase (<em>CAT</em>), superoxide dismutase (<em>SOD</em>), and glutathione peroxidase (<em>GSH-PX</em>), which resulted in significant increase in the cumulative mortality rates.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"308 ","pages":"Article 111898"},"PeriodicalIF":2.1,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144555894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the IGFBP-rP cDNA and its associations with growth traits in Macrobrachium rosenbergii","authors":"Qianqian Ding,&nbsp;Yifan He,&nbsp;Weifeng Gao,&nbsp;Xiaojian Gao,&nbsp;Yao Zhang,&nbsp;Xiaojun Zhang,&nbsp;Qun Jiang","doi":"10.1016/j.cbpa.2025.111901","DOIUrl":"10.1016/j.cbpa.2025.111901","url":null,"abstract":"<div><div>Insulin-like growth factor binding proteins (IGFBPs) are key regulators of insulin-like growth factors (IGFs), which play essential roles in growth and development. In this study, a IGFBP related protein cDNA from <em>Macrobrachium rosenbergii</em> (designated <em>Mr-IGFBP-rP</em>) was identified. The full-length cDNA of <em>Mr-IGFBP-rP</em> is 1494 base pairs (bp) including a 22 bp 5′-untranslated region (UTR), a 707 bp 3’-UTR, and a 765 bp open reading frame (ORF) encoding a 254-amino acid protein. The Mr-IGFBP-rP protein contains three conserved domains: an IGF-binding domain (amino acids 34–89), a Kazal-type serine protease inhibitor domain (102–125), and an immunoglobulin-like C2-type domain (147–245). Sequence analysis revealed that Mr-IGFBP-rP shares 75.74 % identity with the IGFBP-rP1 of <em>Palaemon carinicauda</em> and 63.26 % with that of <em>Penaeus chinensis</em>. Expression analysis showed that <em>Mr-IGFBP-rP</em> is most highly expressed in the abdominal ganglia, followed by gill, heart, and brain. Eleven single nucleotide polymorphisms (SNPs) were identified within <em>Mr-IGFBP-rP</em>. Of these, three SNPs showed significant associations with growth traits in <em>M. rosenbergii</em>. Specifically, individuals with the AA genotype at the 5263-G/A locus exhibited greater body weight. Those with the TT genotype at the 5302-G/T locus showed increased body weight, cephalothorax height, and abdominal segment 1 height. In contrast, the CC genotype at the 5743-G/C locus was associated with reduced cephalothorax length, cephalothorax height, abdominal segment 1 height, and abdominal length. This study lays a foundation for understanding the role of <em>Mr-IGFBP-rP</em> in growth regulation in <em>M. rosenbergii</em>. The identified SNPs hold potential as candidate markers for marker-assisted selection in this species.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111901"},"PeriodicalIF":2.1,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Water pH alters acid-base compensatory responses in white sturgeon (Acipenser transmontanus) exposed to modest and severe environmental hypercapnia","authors":"Ryan B. Shartau ,&nbsp;Daniel W. Baker","doi":"10.1016/j.cbpa.2025.111900","DOIUrl":"10.1016/j.cbpa.2025.111900","url":null,"abstract":"<div><div>Elevated CO₂ (hypercapnia) in fishes induces a respiratory acidosis, which is compensated by branchial acid-base relevant ion transfer. This organismal compensation is hypothesized to be affected by water pH, but assessing this effect is difficult when examining fish species (e.g., rainbow trout <em>Oncorhynchus mykiss</em>, Japanese flounder, <em>Paralichthys olivaceus</em>) that rely on extracellular pH regulation (pHe) to drive recovery as when it fails, mortality may occur quickly. Examining fishes that both perform pHe compensation, but do not require it for survival due to preferential intracellular pH (pH_i) regulation (PPR) such as does the white sturgeon (<em>Acipenser transmontanus</em>), allows us to determine the effect of water pH on acid-base compensation during hypercapnia. White sturgeon were exposed to 48 h of elevated CO₂ tensions while manipulating water pH to determine how water pH can alter patterns of acid-base compensation. Our findings demonstrate that water pH manipulation can have significant effects on the pHe compensatory response. For example, complete pHe compensation was observed in fish exposed to water equilibrated with 1 kPa CO₂, but reducing water pH by ∼1 pH unit prevented complete compensation. Alternately, when sturgeon were exposed to alkalized water equilibrated with 4 kPa CO₂ (i.e., pH of 6.3 compared to 5.7 for water with 4 kPa CO₂), pH compensation was more rapid. Water pH and pHe compensation rates had no effect on pH_i regulation. Our findings demonstrate that water pH can aid or assist pHe compensatory rates at a range of hypercapnic challenges, and so may have contributed to the origin of PPR as a primary strategy for some fish species to protect critical tissues largely independent of environmental conditions.</div></div><div><h3>Summary</h3><div>White sturgeon were exposed to two levels of elevated CO₂ (1 and 4 kPa) in water of two different pH values, and we tracked pH compensatory responses over the following two days. Acidifying water at modest CO₂ levels within the limit for complete compensation reduced the rate at which blood pH recovered. Alkalizing water during severe hypercapnia typically beyond that limit increased both the magnitude and the rate of organismal pH compensation. Intracellular pH protection was unaffected by any treatment.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111900"},"PeriodicalIF":2.1,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of individual differences in anxiety on anti-predator responses, thermal and hypoxia tolerance in zebrafish (Danio rerio)","authors":"Xiao-hong Li,&nbsp;Cheng Fu,&nbsp;Xue-ting Tan,&nbsp;Shi-jian Fu","doi":"10.1016/j.cbpa.2025.111899","DOIUrl":"10.1016/j.cbpa.2025.111899","url":null,"abstract":"<div><div>Anxiety, as a crucial stress response to potential threats in animals, exhibits significant individual differences, with high-anxiety individuals typically allocating greater attentional resources to threat detection. We hypothesized that such individuals would demonstrate enhanced threat sensitivity and anti-predator responses, but compromised stress tolerance due to constrained energy allocation. Using the novel tank diving test, we screened zebrafish (<em>Danio rerio</em>) with high- and low-anxiety phenotypes and compared their anti-predator responses (anxiety-like behaviors, metabolic rates, and predator avoidance behaviors) under predator chemical or visual cues, along with their thermal and hypoxia tolerance capacities under baseline conditions. The results demonstrate that high-anxiety zebrafish exhibit enhanced anti-predator responses in threatening environments, while showing no compromised stress tolerance at baseline. These findings demonstrate the adaptive superiority of the high-anxiety phenotype in high-predation-risk environments, providing theoretical foundations for understanding the evolutionary role of anxiety in fish.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111899"},"PeriodicalIF":2.1,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Temporal changes in PTTH/Egf signaling and ERK target gene expressions during chilling-induced diapause termination in Bombyx mori eggs” [Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, Volume 307, September 2025, 111,884].","authors":"Shi-Hong Gu","doi":"10.1016/j.cbpa.2025.111895","DOIUrl":"10.1016/j.cbpa.2025.111895","url":null,"abstract":"","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111895"},"PeriodicalIF":2.1,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144320967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the fatty acid composition of Pacific bluefin tuna Thunnus orientalis larvae by body part and depending on growth rate","authors":"Yuko Hiraoka ,&nbsp;Hiroshi Hashimoto ,&nbsp;Tetsuya Takatsu ,&nbsp;Yuki Koide ,&nbsp;Yosuke Tanaka ,&nbsp;Yasuhiro Ando","doi":"10.1016/j.cbpa.2025.111897","DOIUrl":"10.1016/j.cbpa.2025.111897","url":null,"abstract":"<div><div>In this study, we aimed to elucidate the characteristics of faster-growing Pacific bluefin tuna (<em>Thunnus orientalis)</em> larvae by focusing on the localization of long-chain polyunsaturated fatty acids in different body parts and at various days post-hatch (dph). To accomplish this, we compared low- and high-growth-rate groups of larvae under mass-rearing conditions. Multivariate analysis revealed that the first axis separated the dph and growth groups, and the high-growth-rate group tended to be distributed on the same side at 20 dph. This suggests that the faster-growing larvae had switched their diet sooner after the change in feeding regime, either from rotifers to yolk-sac larvae of the spangled emperor (<em>Lethrinus nebulosus</em>) or from yolk-sac larvae to a commercial diet. However, the fatty acid compositions differed significantly between the two growth groups at 10 dph, before the change in feeding regime (PERMANOVA; <em>P</em> &lt; 0.001). Notably, %DHA was higher in the head (mean: 24.4 %) and eyes (29.2 %) of the high-growth group, while %DHA levels in the trunk remained similar between groups (range: 23.1 %–23.7 %). These findings suggest that early accumulation of DHA (approximately 30 %) in the eyes, in contrast to the lower requirement in trunk tissues, may be critical for sustaining rapid growth in Pacific bluefin larvae during the pre-diet-shift period.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111897"},"PeriodicalIF":2.1,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Individual differences in response to a chronic high-fat diet in striped hamsters (Cricetulus barabensis)","authors":"Wenting Li ,&nbsp;Binxin Yin ,&nbsp;Xinyuan Dong ,&nbsp;Boyang Ding ,&nbsp;Jing Wen","doi":"10.1016/j.cbpa.2025.111896","DOIUrl":"10.1016/j.cbpa.2025.111896","url":null,"abstract":"<div><div>Adult male striped hamsters (<em>Cricetulus barabensis</em>) were fed an HFD for 12 weeks to examine individual differences in response to chronic exposure to a high-fat diet (HFD). The hamsters were subsequently assigned to three experimental groups according to those that exhibited body weight gain (GW), a stable body weight (SW), or body weight loss (LW). The GW group showed a profile of HFD-induced obesity, with the highest levels of energy intake, fat content, brown adipose tissue (BAT) mass, <em>UCP1</em> gene expression in the BAT, and lipid metabolism. The SW group was resistant to HFD, showing lower levels of the above-mentioned markers than those of the GW group. In addition, the LW group appeared to be aversive to HFD, displaying a negative body mass gain compared to the other two groups. Group differences in the relative abundance of several strains of the gut microbiota were also observed, suggesting the potential involvement of the gut microbiota in individual variation in the sensitivity, resistance, and aversive responses to HFD-induced obesity. These data demonstrate the utility of the striped hamster model in functional studies of the mechanisms underlying HFD-induced obesity and its potential treatment and prevention.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111896"},"PeriodicalIF":2.1,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144364755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Melatonin modulates autophagy, mitochondria and antioxidant in the liver and brain of Perccottus glenni during recovery from freezing” [Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 303, 2025, 111824].","authors":"Cunrun Ye,&nbsp;Zhaoyang Ning,&nbsp;Tingting Hu,&nbsp;Xiaoyu Zhao,&nbsp;Weijie Mu","doi":"10.1016/j.cbpa.2025.111894","DOIUrl":"10.1016/j.cbpa.2025.111894","url":null,"abstract":"","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111894"},"PeriodicalIF":2.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of acute environmental challenges on the expression of peptide hormone-encoding genes in the zebrafish caudal neurosecretory system","authors":"Bérénice Bichon,&nbsp;Gladys Alfama,&nbsp;Anne-Laure Gaillard,&nbsp;Hervé Tostivint,&nbsp;Guillaume Pézeron","doi":"10.1016/j.cbpa.2025.111893","DOIUrl":"10.1016/j.cbpa.2025.111893","url":null,"abstract":"<div><div>The caudal neurosecretory system (CNSS) is a neuroendocrine complex unique to fish, first described in 1955. Since then, it has been hypothesized to contribute to several physiological processes, but its real functions remain largely unclear. However, so far, the many studies devoted to it agree that it could play an important role in osmoregulation. More recently, it has also been suggested that it could be involved in thermal adaptation. The zebrafish (<em>Danio rerio</em>) is a well-established model organism for functional studies. Yet so far, the functions of the CNSS have not been thoroughly investigated in this species. As a first step in such investigations, the present study aimed to identify environmental factors whose variations induce changes in CNSS endocrine activity. For this purpose, juvenile zebrafish were submitted to acute (2, 8, and 24 h) pH, salinity, and temperature challenges. As indicators of the CNSS endocrine activity, the expression levels of peptide hormone-encoding genes known or suspected to be synthesized in the CNSS were measured using quantitative PCR. The genes selected for this study were as follows: <em>corticotropin-releasing hormone b</em> (<em>crhb</em>), oxytocin (<em>oxt</em>), <em>proenkephalin</em> (<em>penka</em> and <em>penkb</em>), <em>parathyroid hormone-like hormone</em> (<em>pthlha</em> and <em>pthlhb</em>), <em>stanniocalcin 2</em> (<em>stc2a</em> and <em>stc2b</em>), <em>urotensin 1</em> (<em>uts1</em>) and <em>urotensin 2</em> (<em>uts2a</em> and <em>uts2b</em>). Our findings revealed that the pH challenge affected the expression of three genes – <em>crhb</em>, <em>penka,</em> and <em>stc2b -</em> and the salinity challenge altered four genes - <em>oxt, uts1, uts2a</em>, and <em>uts2b</em> - while the temperature challenge modified the expression of all genes of our panel. These results indicated that the zebrafish CNSS is sensitive to changes in these environmental parameters and support the use of the zebrafish as a relevant model for studying the functions of the CNSS.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111893"},"PeriodicalIF":2.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144295407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth rate is associated with reduced oxidative stress and this effect is modulated by the degree of social dominance in males of an African cichlid fish","authors":"Peter D. Dijkstra","doi":"10.1016/j.cbpa.2025.111892","DOIUrl":"10.1016/j.cbpa.2025.111892","url":null,"abstract":"<div><div>Attaining large body size has several selective benefits, however, increased growth rate has potential costs that can constrain investment in other life history traits, such as reproductive output and territorial defense. Oxidative stress can both constrain and result from growth, potentially mediating life history trade-offs between growth rate and other life history traits. Studies on the oxidative cost of growth have provided mixed evidence, in part because components of oxidative balance, including oxidative damage and antioxidant function, is influenced by investment in other activities in a tissue-specific manner. Here, I examined how among-individual variation in growth rate is linked to oxidative stress, and how this relationship is influenced by markers of social dominance (aggressiveness and relative gonad size) in males of the cichlid fish <em>Astatotilapa burtoni</em>. To this end, 7 markers of oxidative damage and antioxidant function in various tissue types (total of 14 measurements) were assessed in dominant and subordinate males. I found that dominant males grew faster than subordinate males. However, increased growth was linked to reduced oxidative stress. This effect was independent of social status but modulated by the degree of social dominance. Overall, the results are consistent with oxidative stress mediating the link between growth and other life history traits. However, my findings challenge the idea that increased growth rate results in elevated oxidative stress, perhaps due to effective protective mechanisms that can neutralize the oxidative challenge of growth.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111892"},"PeriodicalIF":2.1,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144259425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}