Pub Date : 2023-09-30DOI: 10.37360/blacpma.23.22.5.50
Maria del Rosario Huizar Lopez, Josefina Casas Solis, Itzel Citlalli Alcazar Rios, Lucia Barrientos Ramirez, A. Santerre
Annona muricataLinn. (Annonaceae) is a tropical plant with multiple beneficial health effects including anticancer properties. In breast cancer patients, overexpression of the HER2 oncoprotein corresponds to a poor prognosis, thus the main purpose of this study was to evaluate the cytotoxicity of ethanolic extracts from dried and fresh leaf of A. muricataon HER2+ breast cancer cells. MTT assays were performed and IC50determined in HCC1954 (HER2+) cells, as well as in MCF7 (HER-) and peripheral blood mononuclear cells (PBMC) used as controls. Total polyphenol content evaluation and phytochemical screening were also performed. The cytotoxic effect of A. muricataextracts (125-1000 μg/mL) was dose-dependent and cell-type specific. The extracts exhibited higher cytotoxicity against HCC1954 than MCF7 cells, but weak toxicity against PBMC. This is the first report of the cytotoxic effect of A. muricataon HCC1954 cells, highlighting its potential for treating anti-estrogen-resistant breast cancers and low toxicity against PBMC.
{"title":"Selective cytotoxic effect of Annona muricataL. in HCC1954 (HER2+) breast cancer cells","authors":"Maria del Rosario Huizar Lopez, Josefina Casas Solis, Itzel Citlalli Alcazar Rios, Lucia Barrientos Ramirez, A. Santerre","doi":"10.37360/blacpma.23.22.5.50","DOIUrl":"https://doi.org/10.37360/blacpma.23.22.5.50","url":null,"abstract":"Annona muricataLinn. (Annonaceae) is a tropical plant with multiple beneficial health effects including anticancer properties. In breast cancer patients, overexpression of the HER2 oncoprotein corresponds to a poor prognosis, thus the main purpose of this study was to evaluate the cytotoxicity of ethanolic extracts from dried and fresh leaf of A. muricataon HER2+ breast cancer cells. MTT assays were performed and IC50determined in HCC1954 (HER2+) cells, as well as in MCF7 (HER-) and peripheral blood mononuclear cells (PBMC) used as controls. Total polyphenol content evaluation and phytochemical screening were also performed. The cytotoxic effect of A. muricataextracts (125-1000 μg/mL) was dose-dependent and cell-type specific. The extracts exhibited higher cytotoxicity against HCC1954 than MCF7 cells, but weak toxicity against PBMC. This is the first report of the cytotoxic effect of A. muricataon HCC1954 cells, highlighting its potential for treating anti-estrogen-resistant breast cancers and low toxicity against PBMC.","PeriodicalId":55342,"journal":{"name":"Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas","volume":"44 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84545852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the high Andean areas, the main economic activity is alpaca raising, which is affected by various infectious and parasitic diseases. Rural populations often resort to wild plants that have diverse properties and help control various diseases. The objective was to document the uses of wild plants in the control of alpaca diseases in the high Andean areas of the Puno and Arequipa regions. Fifty alpaca-breeding families were interviewed in five localities. Thirty-two species belonging to 16 families were reported, with the Asteraceae and Fabaceae families having the highest number of species. The most frequently treated pathologies were diarrhea, pneumonia, fever and enteric parasitosis. For diarrhea treatment, the most used plants were M. mollis, S. nutansand T. filifolia, for pneumonia were G. prostrataand G. viravira, for enteric parasitosis were B. tricuneataand L. daucifoliaand for the elimination of ectoparasites (lice) was A. compacta. For all diseases, the treatment dose was higher in adults than in neonates and its application is in the rainy season. In acute disease conditions, rural families choice to drugs. Wild plants are a viable and sustainable alternative for the treatment of various diseases in alpacas.
{"title":"Ancestral knowledge on the use of wild plants for the treatment of diseases in alpacas","authors":"Ivon Gutierrez-Flores, Á. Canales-Gutiérrez, Katia Pillco-Mamani, Moises Mamani-Mamani, M. Chambi-Alarcon, Danitza Caceres-Garcia","doi":"10.37360/blacpma.23.22.5.46","DOIUrl":"https://doi.org/10.37360/blacpma.23.22.5.46","url":null,"abstract":"In the high Andean areas, the main economic activity is alpaca raising, which is affected by various infectious and parasitic diseases. Rural populations often resort to wild plants that have diverse properties and help control various diseases. The objective was to document the uses of wild plants in the control of alpaca diseases in the high Andean areas of the Puno and Arequipa regions. Fifty alpaca-breeding families were interviewed in five localities. Thirty-two species belonging to 16 families were reported, with the Asteraceae and Fabaceae families having the highest number of species. The most frequently treated pathologies were diarrhea, pneumonia, fever and enteric parasitosis. For diarrhea treatment, the most used plants were M. mollis, S. nutansand T. filifolia, for pneumonia were G. prostrataand G. viravira, for enteric parasitosis were B. tricuneataand L. daucifoliaand for the elimination of ectoparasites (lice) was A. compacta. For all diseases, the treatment dose was higher in adults than in neonates and its application is in the rainy season. In acute disease conditions, rural families choice to drugs. Wild plants are a viable and sustainable alternative for the treatment of various diseases in alpacas.","PeriodicalId":55342,"journal":{"name":"Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas","volume":"21 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90625021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.37360/blacpma.23.22.5.41
Sidra Altaf, R. Z. Abbas, T. Akhtar, F. Siddique, M. S. Mahmood, Muhammad Kasib Khan, Khurram Ziaf, M. Rafay, Muhammad Azam Khan, A. Abbas, Tauseef ur Rehman, Liliana Aguilar Marcelino, M. Zia, H. Khater, N. Saeed, Karim Yar Abbasi
Oxidative stress is a key cause of gastrointestinal disorders, primarily stomach ulcers. Multiple intrinsic and extrinsic mechanisms caused the body to produce reactive oxygen species (ROS). The body's antioxidant defense system protects against these reactive species. When the degree of ROS production exceeds the normal range, the body's natural defense system fails to neutralize these dangerous free radicals, necessitating need foran exogenous source of natural antioxidants. Natural herbal remedies have been widely employed as antioxidants to relieve oxidative stress in gastric ulcers. Polyphenols, tannins, essential oils, flavonoids, notably quercetin, carotenoids, vitamin C, vitamin A, and minerals are among the molecules of immense interest in bioassays due to their significant antioxidant effects. In the present review, several natural anti-ulcer medicinal plants along with their antioxidative mechanism have been reported. Electronic databases including PubMed, Google Scholar and Scopus were explored to identify the antioxidant and gastroprotective potential of all the plants.
{"title":"Antioxidant rich medicinal plants as a potential candidate to treat gastric ulcer","authors":"Sidra Altaf, R. Z. Abbas, T. Akhtar, F. Siddique, M. S. Mahmood, Muhammad Kasib Khan, Khurram Ziaf, M. Rafay, Muhammad Azam Khan, A. Abbas, Tauseef ur Rehman, Liliana Aguilar Marcelino, M. Zia, H. Khater, N. Saeed, Karim Yar Abbasi","doi":"10.37360/blacpma.23.22.5.41","DOIUrl":"https://doi.org/10.37360/blacpma.23.22.5.41","url":null,"abstract":"Oxidative stress is a key cause of gastrointestinal disorders, primarily stomach ulcers. Multiple intrinsic and extrinsic mechanisms caused the body to produce reactive oxygen species (ROS). The body's antioxidant defense system protects against these reactive species. When the degree of ROS production exceeds the normal range, the body's natural defense system fails to neutralize these dangerous free radicals, necessitating need foran exogenous source of natural antioxidants. Natural herbal remedies have been widely employed as antioxidants to relieve oxidative stress in gastric ulcers. Polyphenols, tannins, essential oils, flavonoids, notably quercetin, carotenoids, vitamin C, vitamin A, and minerals are among the molecules of immense interest in bioassays due to their significant antioxidant effects. In the present review, several natural anti-ulcer medicinal plants along with their antioxidative mechanism have been reported. Electronic databases including PubMed, Google Scholar and Scopus were explored to identify the antioxidant and gastroprotective potential of all the plants.","PeriodicalId":55342,"journal":{"name":"Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas","volume":"34 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84220443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.37360/blacpma.23.22.5.43
L. Suárez-Rebaza, R. D. de Albuquerque, Ewaldo Zavala, Pedro M Alva-Placencia, Mayer M. Ganoza-Suárez, M. L. Ganoza-Yupanqui, Rainer W Bussmann
The purpose of this work was to evaluate the antioxidant capacity of Prosopis pallida(Fabaceae) fruits “algarrobo”, in addition to determine their main chemical components. Fruit purified extracts from the regions of Tumbes, Piura, Lambayeque, and La Libertad (Peru) were evaluated using infusion, decoction, and alcoholic extraction at concentrations of 45, 70 and 96%. To measure the antioxidant capacity of the extracts, the determination of total phenolics content was performed, followed by the antioxidant evaluation using DPPH, FRAP and ABTS. The chemical identification was carried out through UHPLC-UV-MS/MS. The results showed that the decoction of the sample from the region of La Libertad, as well as the ethanol extracts at 45% of the samples from Tumbes and Lambayeque presented greater antioxidant capacity. Moreover, ten major substances of these extracts were identified: nine phenolic derivatives (vicenin II as the main constituent of the extracts) and one diterpene (7-oxodehydroabietic acid).
{"title":"Chemical composition and antioxidant capacity of purified extracts of Prosopis pallida(Humb. & Bonpl. ex Willd.) Kunth (Fabaceae) fruits from Northern Peru","authors":"L. Suárez-Rebaza, R. D. de Albuquerque, Ewaldo Zavala, Pedro M Alva-Placencia, Mayer M. Ganoza-Suárez, M. L. Ganoza-Yupanqui, Rainer W Bussmann","doi":"10.37360/blacpma.23.22.5.43","DOIUrl":"https://doi.org/10.37360/blacpma.23.22.5.43","url":null,"abstract":"The purpose of this work was to evaluate the antioxidant capacity of Prosopis pallida(Fabaceae) fruits “algarrobo”, in addition to determine their main chemical components. Fruit purified extracts from the regions of Tumbes, Piura, Lambayeque, and La Libertad (Peru) were evaluated using infusion, decoction, and alcoholic extraction at concentrations of 45, 70 and 96%. To measure the antioxidant capacity of the extracts, the determination of total phenolics content was performed, followed by the antioxidant evaluation using DPPH, FRAP and ABTS. The chemical identification was carried out through UHPLC-UV-MS/MS. The results showed that the decoction of the sample from the region of La Libertad, as well as the ethanol extracts at 45% of the samples from Tumbes and Lambayeque presented greater antioxidant capacity. Moreover, ten major substances of these extracts were identified: nine phenolic derivatives (vicenin II as the main constituent of the extracts) and one diterpene (7-oxodehydroabietic acid).","PeriodicalId":55342,"journal":{"name":"Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas","volume":"43 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89910909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.37360/blacpma.23.22.5.42
Adyelle Dantas Ribeiro, Maria Novélia Amaral Cardoso, Julliana Cariry Palhano Freire, Ernani Canuto Figueirêdo Júnior, Mariana Mélani Alexandrino Costa, Priscila Guimaraes Silva, Daliana Queiroga de Castro Gomes, Edja Maria Melo de Brito, Jozinete Vieira Pereira
Limonene is the main component of citrus essential oils, and can reach a concentration of up to 96%. Popularly known for its potential therapeutic effects on the body, among these we point out its broad antimicrobial activity against various types of pathogens. Therefore, the purpose of this study was to address the antimicrobial and antifungal properties of limonene compared to microorganisms of interest in dentistry, based on a bibliometric study and literature review. The following databases were analyzed: PubMed, Google Scholar, SciELO and Science Direct, from which ten articles published between 2011-2021 were selected. Most of results indicate a satisfactory antimicrobial potential, besides providing important data and perspectives regarding the indication and clinical use, in addition to innovative therapeutic modalities for diseases that affect the oral cavity, such as tooth decay, periodontal disease and candidosis.
{"title":"Antimicrobial activity of limonene: Integrative review","authors":"Adyelle Dantas Ribeiro, Maria Novélia Amaral Cardoso, Julliana Cariry Palhano Freire, Ernani Canuto Figueirêdo Júnior, Mariana Mélani Alexandrino Costa, Priscila Guimaraes Silva, Daliana Queiroga de Castro Gomes, Edja Maria Melo de Brito, Jozinete Vieira Pereira","doi":"10.37360/blacpma.23.22.5.42","DOIUrl":"https://doi.org/10.37360/blacpma.23.22.5.42","url":null,"abstract":"Limonene is the main component of citrus essential oils, and can reach a concentration of up to 96%. Popularly known for its potential therapeutic effects on the body, among these we point out its broad antimicrobial activity against various types of pathogens. Therefore, the purpose of this study was to address the antimicrobial and antifungal properties of limonene compared to microorganisms of interest in dentistry, based on a bibliometric study and literature review. The following databases were analyzed: PubMed, Google Scholar, SciELO and Science Direct, from which ten articles published between 2011-2021 were selected. Most of results indicate a satisfactory antimicrobial potential, besides providing important data and perspectives regarding the indication and clinical use, in addition to innovative therapeutic modalities for diseases that affect the oral cavity, such as tooth decay, periodontal disease and candidosis.","PeriodicalId":55342,"journal":{"name":"Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas","volume":"13 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75132372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.37360/blacpma.23.22.5.49
Julio Vilaboa Arroniz, Diego Esteban Platas Rosado, Pedro Zetina Cordoba, Erika Maria Gasperin Garcia, Cristhian Adolfo Velazquez Muñoz, Jose Luis Santiago Hernandez
Medicinal plants (HMS) have been part of everyday life in Mexico, despite being popular, little has been documented about their knowledge and use. A diagnosis was made to know the perception of the general public, housewives, doctors, teachers and people dedicated to herbalism about medicinal plants (HMS). The instruments of the poll (100), the survey (130), the census (37) and the interview (6) were used in the sectors of the population mentioned above. Descriptive statistics and measures of central tendency were used. It is a transgenerational knowledge, transmitted verbally and is part of everyday life. Its use is through infusions (tea) fordiseases considered mild. This knowledge has been relegated to the elderly and people with less purchasing power. However, 100% of the surveyed population knows and/or has used HMS. As empirical knowledge becomes scientific, it will be possible to know its effects on human health, therefore, it is important to carry out experimental studies on the biological effects of plants. In this way, its use could be further expanded through appropriate public policies.
{"title":"Knowledge and use of medicinal plants in Calpan, Puebla, Mexico: The perception of various social sectors","authors":"Julio Vilaboa Arroniz, Diego Esteban Platas Rosado, Pedro Zetina Cordoba, Erika Maria Gasperin Garcia, Cristhian Adolfo Velazquez Muñoz, Jose Luis Santiago Hernandez","doi":"10.37360/blacpma.23.22.5.49","DOIUrl":"https://doi.org/10.37360/blacpma.23.22.5.49","url":null,"abstract":"Medicinal plants (HMS) have been part of everyday life in Mexico, despite being popular, little has been documented about their knowledge and use. A diagnosis was made to know the perception of the general public, housewives, doctors, teachers and people dedicated to herbalism about medicinal plants (HMS). The instruments of the poll (100), the survey (130), the census (37) and the interview (6) were used in the sectors of the population mentioned above. Descriptive statistics and measures of central tendency were used. It is a transgenerational knowledge, transmitted verbally and is part of everyday life. Its use is through infusions (tea) fordiseases considered mild. This knowledge has been relegated to the elderly and people with less purchasing power. However, 100% of the surveyed population knows and/or has used HMS. As empirical knowledge becomes scientific, it will be possible to know its effects on human health, therefore, it is important to carry out experimental studies on the biological effects of plants. In this way, its use could be further expanded through appropriate public policies.","PeriodicalId":55342,"journal":{"name":"Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas","volume":"16 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87042623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.37360/blacpma.23.22.5.47
Edmundo Venegas-Casanova, Jose G Gavidia-Valencia, Yuri F Curo-Vallejos, Miguel Rios, Luis A Chavez-Abanto, Ruben J Aro-Diaz, Jorge Escobar, Felipe Ruben Rubio-Lopez, Jose Uribe-Villarreal, Jose L. Martinez, R. Rengifo-Penadillos
The conversion factor of nitrogen to proteins and isoflavones present in Glycine maxwas determined. For the determination of the conversion factor, we worked with solubilizing the proteins at alkaline pH and then extracting them with acidic pH. The proteins were identified by the Kjeldahl method. The antioxidant capacity was determined after extracting the isoflavones and their glycosides through the Soxhlet method, and then using the Brand Williams method (DPPH). The results indicate that the protein conversion factor was 5.85, the maximum concentration of total isoflavones was 33.33%, the antiradical efficiency of total isoflavones was 0.004 mL/ugmin, the antiradical efficiency of gallic acid was 0.005 mL/ugmin. and the antiradical efficiency of tannic acid was 0.0004 mL/ugmin. These results justify the consumption of Glycine max(Soya)as a food that has a high nutritional quality and provides an excellent source of antioxidants, which will prevent hormonal and carcinogenic diseases.
{"title":"Protein conversion factor and antioxidant capacityof isoflavones in Glycine max","authors":"Edmundo Venegas-Casanova, Jose G Gavidia-Valencia, Yuri F Curo-Vallejos, Miguel Rios, Luis A Chavez-Abanto, Ruben J Aro-Diaz, Jorge Escobar, Felipe Ruben Rubio-Lopez, Jose Uribe-Villarreal, Jose L. Martinez, R. Rengifo-Penadillos","doi":"10.37360/blacpma.23.22.5.47","DOIUrl":"https://doi.org/10.37360/blacpma.23.22.5.47","url":null,"abstract":"The conversion factor of nitrogen to proteins and isoflavones present in Glycine maxwas determined. For the determination of the conversion factor, we worked with solubilizing the proteins at alkaline pH and then extracting them with acidic pH. The proteins were identified by the Kjeldahl method. The antioxidant capacity was determined after extracting the isoflavones and their glycosides through the Soxhlet method, and then using the Brand Williams method (DPPH). The results indicate that the protein conversion factor was 5.85, the maximum concentration of total isoflavones was 33.33%, the antiradical efficiency of total isoflavones was 0.004 mL/ugmin, the antiradical efficiency of gallic acid was 0.005 mL/ugmin. and the antiradical efficiency of tannic acid was 0.0004 mL/ugmin. These results justify the consumption of Glycine max(Soya)as a food that has a high nutritional quality and provides an excellent source of antioxidants, which will prevent hormonal and carcinogenic diseases.","PeriodicalId":55342,"journal":{"name":"Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas","volume":"59 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85120919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.37360/blacpma.23.22.5.48
Angeles Mora-Villa, Antonio Estrella-Parra, G. Ávila-Acevedo, M. Jiménez-Estrada, R. Lira-Saade, T. Hernandez-Delgado
Phytochemical bio-guided studies are used to find compounds with biological activity. Flavonoids from seeds of Leucaenaspecies have antimicrobial activity in strains of medical interest, therefore, fresh seeds were collected from the town of Tlayacapan, Morelos, Mexico. The methanolic extracts were obtained by the maceration technique, targeted fractionation was performed using adsorption and molecular exclusion chromatographic techniques; to observe the antimicrobial activity, agardiffusion techniques were used; spectrometric and spectroscopic techniques were used for the characterization of D-pinitol, resulting from the fractionation of L. leucocephala. Antimicrobial activity was found on strains of Escherichia coliCUSI and Staphylococcus aureusATCC 29213 of the most polar fractions, identifying the responsible compounds by HPLC: caffeic acid, gallic acid, p-coumaric acid, quercetin, catechin and apigenin, these compounds can inhibit the activation enzymatic, synthesis of nucleicacids and proteins, chelating with different ions, etc.
{"title":"Bio-guided study of secondary metabolites with antimicrobial activity and isolation of D-pinitol from Leucaena esculentaand L. leucocephalaseeds","authors":"Angeles Mora-Villa, Antonio Estrella-Parra, G. Ávila-Acevedo, M. Jiménez-Estrada, R. Lira-Saade, T. Hernandez-Delgado","doi":"10.37360/blacpma.23.22.5.48","DOIUrl":"https://doi.org/10.37360/blacpma.23.22.5.48","url":null,"abstract":"Phytochemical bio-guided studies are used to find compounds with biological activity. Flavonoids from seeds of Leucaenaspecies have antimicrobial activity in strains of medical interest, therefore, fresh seeds were collected from the town of Tlayacapan, Morelos, Mexico. The methanolic extracts were obtained by the maceration technique, targeted fractionation was performed using adsorption and molecular exclusion chromatographic techniques; to observe the antimicrobial activity, agardiffusion techniques were used; spectrometric and spectroscopic techniques were used for the characterization of D-pinitol, resulting from the fractionation of L. leucocephala. Antimicrobial activity was found on strains of Escherichia coliCUSI and Staphylococcus aureusATCC 29213 of the most polar fractions, identifying the responsible compounds by HPLC: caffeic acid, gallic acid, p-coumaric acid, quercetin, catechin and apigenin, these compounds can inhibit the activation enzymatic, synthesis of nucleicacids and proteins, chelating with different ions, etc.","PeriodicalId":55342,"journal":{"name":"Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas","volume":"19 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86233855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.37360/blacpma.23.22.5.45
Lisseth Carolina Espinosa Espinoza, Melissa Salinas Rivera, N. Bec, Jorge Ramirez Robles, C. Larroque, Chabaco Armijos
The aim of this study was to identify the chemical composition of the Piper arboreumAubl. essential oil (EO), and to evaluate its inhibitory activity in vitroagainst the enzymes butyrylcholinesterase (BuChE) and acetylcholinesterase (AChE). The EO was obtained by steam distillation of the leaves, which were collected in Pindal canton of the Loja province in southern Ecuador. The chemical composition was analyzed using the gas chromatography technique coupled to a mass spectrometry detector (GC-MS) and gas chromatography coupled to a flame ionization detector (GC-FID). A total of 41 compounds were identified, the major components found in the oil were limonene (31,46%), β-selinene (12,01%), (E)-caryophyllene (7,53%), bicyclogermacrene (6,72%), germacrene D (3,83%) and β-elemene (3,63%). In in vitro analyzes, the EO showed high selective inhibition for BuChE with an IC50inhibition value of 29,3±3,3 μg/mL. By contrast, the EO was not active against the AChE enzyme (IC50was 100,1±15,2 μg/mL).
{"title":"Chemical composition and selective BuChE activity of the essential oil of Piper arboreum Aubl. from Ecuador","authors":"Lisseth Carolina Espinosa Espinoza, Melissa Salinas Rivera, N. Bec, Jorge Ramirez Robles, C. Larroque, Chabaco Armijos","doi":"10.37360/blacpma.23.22.5.45","DOIUrl":"https://doi.org/10.37360/blacpma.23.22.5.45","url":null,"abstract":"The aim of this study was to identify the chemical composition of the Piper arboreumAubl. essential oil (EO), and to evaluate its inhibitory activity in vitroagainst the enzymes butyrylcholinesterase (BuChE) and acetylcholinesterase (AChE). The EO was obtained by steam distillation of the leaves, which were collected in Pindal canton of the Loja province in southern Ecuador. The chemical composition was analyzed using the gas chromatography technique coupled to a mass spectrometry detector (GC-MS) and gas chromatography coupled to a flame ionization detector (GC-FID). A total of 41 compounds were identified, the major components found in the oil were limonene (31,46%), β-selinene (12,01%), (E)-caryophyllene (7,53%), bicyclogermacrene (6,72%), germacrene D (3,83%) and β-elemene (3,63%). In in vitro analyzes, the EO showed high selective inhibition for BuChE with an IC50inhibition value of 29,3±3,3 μg/mL. By contrast, the EO was not active against the AChE enzyme (IC50was 100,1±15,2 μg/mL).","PeriodicalId":55342,"journal":{"name":"Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas","volume":"29 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73033669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-30DOI: 10.37360/blacpma.23.22.4.31
Lucie Pekova, J. Žiarovská, Eloy Fernández-Cusimamani
The present study aimed to document the traditional use of medicinal plants used to treat Diabetes mellitus Type II in Bolivia. Based on the scientific literature were identified 35 medicinal plant species distributed in 21 botanical families, of which 52 % are native to Bolivia and 48 % are introduced. The botanical families with the highest representation of species were Asteraceae (7 species, 19%) and Fabaceae (17%). The most frequented growth forms were herbs (34%) and trees (29%). Leaves (30%) were the most frequently used plant parts, followed by roots (14%), and bark (9%), mostly prepared as an infusion (40%) and decoction (33%). From the available scientific studies, 25 medicinal species were verified for their antidiabetic properties with positive results, but it is necessary provide more biochemical and clinical analysis of medicinal plants to make better use of their potential.
{"title":"Medicinal plants with antidiabetic activity used in the traditional medicine in Bolivia: A review","authors":"Lucie Pekova, J. Žiarovská, Eloy Fernández-Cusimamani","doi":"10.37360/blacpma.23.22.4.31","DOIUrl":"https://doi.org/10.37360/blacpma.23.22.4.31","url":null,"abstract":"The present study aimed to document the traditional use of medicinal plants used to treat Diabetes mellitus Type II in Bolivia. Based on the scientific literature were identified 35 medicinal plant species distributed in 21 botanical families, of which 52 % are native to Bolivia and 48 % are introduced. The botanical families with the highest representation of species were Asteraceae (7 species, 19%) and Fabaceae (17%). The most frequented growth forms were herbs (34%) and trees (29%). Leaves (30%) were the most frequently used plant parts, followed by roots (14%), and bark (9%), mostly prepared as an infusion (40%) and decoction (33%). From the available scientific studies, 25 medicinal species were verified for their antidiabetic properties with positive results, but it is necessary provide more biochemical and clinical analysis of medicinal plants to make better use of their potential.","PeriodicalId":55342,"journal":{"name":"Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas","volume":"53 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81451841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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