Aim. To study the activity of nucleolar organizer regions (NORs) in different Ukrainian cattle breeds in terms of their apparent activity status in silver stain and possible relation with milk productivity. Methods. Chromosome prepara- tions using lymphocytes from the peripheral blood of 90 cows of different breeds were used in the study. NOR activity was determined by visual evaluation of concentrations of silver precipitation on NORs in individual chromosomes. A 50 % silver nitrate solution was used to stain chromosome preparations. NORs were detected as dark spots on telomeres of the corresponding chromosomes. Results. The cytological analysis of chromosome preparations from lymphocytes of first lactation cows detected NOR polymorphism in Ukrainian Red-and-Motley dairy cattle (URM), Ukrainian Black-and-White dairy breed (UBW), and hybrid cows, obtained by crossing Ukrainian Red-and-Motley dairy breed and Montbeliarde bulls (URM × M). First lactation cows of URM and UBM had higher or the same inci- dence of cells with four (29.8 and 30 %) and five (17.1 and 19.5 %) NORs, while in URM × M cows the incidence of cells with the same number of NORs was almost twice lower; cells with 7 and 8 NORs exceeded a similar index for other investigated breeds almost twice (2.5 against 4.5 % and 2.0 against 4.2 %). The highest level of chromosomal aberrations (CA) was observed in the group of animals with medium number (2 to 3 NORs per cell), and the lowest – in the group with a high number of NORs (from 6 to 7) with a reliable intergroup difference (p < 0.01). NOR activity was the highest in the group of animals of local origin (URM × M) with a milk yield over 7,000 kg in 305 days of the first lactation and the lowest in the UBW cows with a milk yield of 4–5,000 kg during the first lactation. Conclusions. We determined the differences in the activity of nucleolar organizers between the investigated groups of cows of dairy breed. URM × M hybrids reliably (р ≤ 0.05) exceeded dairy UBW cows by this index. No statistically significant different was found between other investigated groups of animals by this trait. Higher dairy productivity was found in the animals with higher frequency of NORs in the chromosomes of metaphase cells. In our opinion, the number of active NORs demonstrates relative variability between their number and the rate of protein synthesis, required to implement the productivity traits of the investigated animals.
{"title":"Polymorphism of nucleolar organizer regions in different Ukrainian cattle breeds","authors":"V. Dzitsiuk, H. Typylo, I. Mitiohlo","doi":"10.15407/agrisp8.01.024","DOIUrl":"https://doi.org/10.15407/agrisp8.01.024","url":null,"abstract":"Aim. To study the activity of nucleolar organizer regions (NORs) in different Ukrainian cattle breeds in terms of their\u0000apparent activity status in silver stain and possible relation with milk productivity. Methods. Chromosome prepara-\u0000tions using lymphocytes from the peripheral blood of 90 cows of different breeds were used in the study. NOR activity\u0000was determined by visual evaluation of concentrations of silver precipitation on NORs in individual chromosomes.\u0000A 50 % silver nitrate solution was used to stain chromosome preparations. NORs were detected as dark spots on\u0000telomeres of the corresponding chromosomes. Results. The cytological analysis of chromosome preparations from\u0000lymphocytes of first lactation cows detected NOR polymorphism in Ukrainian Red-and-Motley dairy cattle (URM),\u0000Ukrainian Black-and-White dairy breed (UBW), and hybrid cows, obtained by crossing Ukrainian Red-and-Motley\u0000dairy breed and Montbeliarde bulls (URM × M). First lactation cows of URM and UBM had higher or the same inci-\u0000dence of cells with four (29.8 and 30 %) and five (17.1 and 19.5 %) NORs, while in URM × M cows the incidence of\u0000cells with the same number of NORs was almost twice lower; cells with 7 and 8 NORs exceeded a similar index for\u0000other investigated breeds almost twice (2.5 against 4.5 % and 2.0 against 4.2 %). The highest level of chromosomal\u0000aberrations (CA) was observed in the group of animals with medium number (2 to 3 NORs per cell), and the lowest –\u0000in the group with a high number of NORs (from 6 to 7) with a reliable intergroup difference (p < 0.01). NOR activity\u0000was the highest in the group of animals of local origin (URM × M) with a milk yield over 7,000 kg in 305 days of the\u0000first lactation and the lowest in the UBW cows with a milk yield of 4–5,000 kg during the first lactation. Conclusions.\u0000We determined the differences in the activity of nucleolar organizers between the investigated groups of cows of dairy\u0000breed. URM × M hybrids reliably (р ≤ 0.05) exceeded dairy UBW cows by this index. No statistically significant different was found between other investigated groups of animals by this trait. Higher dairy productivity was found in the\u0000animals with higher frequency of NORs in the chromosomes of metaphase cells. In our opinion, the number of active\u0000NORs demonstrates relative variability between their number and the rate of protein synthesis, required to implement\u0000the productivity traits of the investigated animals.","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2021-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44363322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. To identify and evaluate allele frequencies of Ppd-D1a, Ppd-B1a, Ppd-B1c and Ppd-1 of the genotypes of spring bread wheat varieties from various climatic zones. Methods. DNA isolation, allele-specific PCR, electrophoresis in agarose and polyacrylamide gel, statistical analysis. Results. 137 varieties of spring bread wheat of various origin were detected to identify Ppd-1 genotypes of Ppd-D1a, Ppd-B1a and Ppd-B1c allele carriers. The results for the total sampling of the varieties under investigation and the sampling of Asian varieties yielded six different Ppd-1 genotypes in each. As for samplings of other regions, there were from two (Mexico) to four (Europe, the USA, Canada, Ukraine) Ppd-1 genotypes. In the total sampling of varieties, there was a high incidence (20.5 %) of genotypes, dominant only in allele Ppd-D1a, varying from 0 (Russia) to 85.0 % (Mexico). The incidence of the genotypes with monogenically dominant Ppd-B1a (7.3 %) or Ppd-B1c (5.1 %) in the total sampling, was considerably lower. These genotypes were most common for the sampling of the varieties from the USA and Canada (25.0 and 16.7 % respectively). Digenically dominant Ppd-D1a Ppd-B1a genotypes were found in the total sampling with relatively low incidence (7.3 %), and were notable for the varieties from Asia (33.4 %), Mexico (15.0 %), Ukraine (13.1 %), and Europe (3.1 %). The di genically dominant genotype Ppd-D1a Ppd-B1с was found only in the Japanese variety Konosu-25. Gene Ppd-A1 was present in all the spring varieties under investigation in its recessive state. Conclusions. Out of three dominant alleles in the studied sampling, the highest incidence was noted for allele Ppd-D1a (28.5 %). All the varieties from Mexico, present in the set, carry this allele. At the same time, it was not found in any variety from Russia. Allele Ppd-B1a was detected in the varieties from all the regions with the incidence of 7.7 (Russia) – 44.4 % (Asia). Allele Ppd-B1c was sporadically present in the varieties from Russia, Ukraine, the USA, Japan, and Brazil, and its incidence in the total sampling was insignificant (5.8 %). The varieties, identified by the allelic status of Ppd-1 genes, may be used as donors for selection and determination of the influence of alleles for each gene by the development rate and related economically valuable traits of bread wheat.
{"title":"Allele frequencies of Ppd-D1a, Ppd-B1a, and Ppd-B1c of photoperiodic sensitivity genes in spring bread wheat varieties (Triticum aestivum L.) of various origin","authors":"I. Balashova, V. Fait","doi":"10.15407/agrisp8.01.003","DOIUrl":"https://doi.org/10.15407/agrisp8.01.003","url":null,"abstract":"Aim. To identify and evaluate allele frequencies of Ppd-D1a, Ppd-B1a, Ppd-B1c and Ppd-1 of the genotypes of spring\u0000bread wheat varieties from various climatic zones. Methods. DNA isolation, allele-specific PCR, electrophoresis in\u0000agarose and polyacrylamide gel, statistical analysis. Results. 137 varieties of spring bread wheat of various origin\u0000were detected to identify Ppd-1 genotypes of Ppd-D1a, Ppd-B1a and Ppd-B1c allele carriers. The results for the total\u0000sampling of the varieties under investigation and the sampling of Asian varieties yielded six different Ppd-1 genotypes\u0000in each. As for samplings of other regions, there were from two (Mexico) to four (Europe, the USA, Canada, Ukraine)\u0000Ppd-1 genotypes. In the total sampling of varieties, there was a high incidence (20.5 %) of genotypes, dominant only\u0000in allele Ppd-D1a, varying from 0 (Russia) to 85.0 % (Mexico). The incidence of the genotypes with monogenically\u0000dominant Ppd-B1a (7.3 %) or Ppd-B1c (5.1 %) in the total sampling, was considerably lower. These genotypes were\u0000most common for the sampling of the varieties from the USA and Canada (25.0 and 16.7 % respectively). Digenically\u0000dominant Ppd-D1a Ppd-B1a genotypes were found in the total sampling with relatively low incidence (7.3 %), and\u0000were notable for the varieties from Asia (33.4 %), Mexico (15.0 %), Ukraine (13.1 %), and Europe (3.1 %). The di\u0000genically dominant genotype Ppd-D1a Ppd-B1с was found only in the Japanese variety Konosu-25. Gene Ppd-A1 was\u0000present in all the spring varieties under investigation in its recessive state. Conclusions. Out of three dominant alleles\u0000in the studied sampling, the highest incidence was noted for allele Ppd-D1a (28.5 %). All the varieties from Mexico,\u0000present in the set, carry this allele. At the same time, it was not found in any variety from Russia. Allele Ppd-B1a was\u0000detected in the varieties from all the regions with the incidence of 7.7 (Russia) – 44.4 % (Asia). Allele Ppd-B1c was\u0000sporadically present in the varieties from Russia, Ukraine, the USA, Japan, and Brazil, and its incidence in the total\u0000sampling was insignificant (5.8 %). The varieties, identified by the allelic status of Ppd-1 genes, may be used as donors\u0000for selection and determination of the influence of alleles for each gene by the development rate and related economically valuable traits of bread wheat.","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2021-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49055065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Veretsun, B. Stegniy, O. Rula, V. Bolotin, A. Stegniy, A. Gerilovych, D. Muzyka
Aim. To conduct a virological, PCR, PCR-RFLP and sequencing study of infectious laryngotracheitis virus (ILTV) isolates obtained from sick and dead chickens at industrial and backyard poultry farms in the eastern region of Ukraine collected over the years 2010–2019 and to establish their pathotype and relationship with internationally occurring strains. Methods. Material for virological studies was collected in the framework of research program of the NSC IEСVM during 2010-2019 in the poultry farms in the North-Eastern region of Ukraine, where the birds with the respiratory clinical signs were found. In total, 28 poultry farms were observed. ILTV isolates were obtained with conventional methods, using 10–12-day-old chicken embryos. A 0,2 ml of 10–20 % suspension of pathological material in PBS was used for inoculation. For in-depth studies, we used 4 isolates of ILTV obtained from sick and dead chickens from industrial and backyard poultry farms in Kharkiv, Luhansk, Donetsk, and Sumy regions from 2010–2019. The identification of ILTV isolates was performed via conventional PCR. The pathotype of ILTV strains was determined using PCR-RFLP (polymerase chain reaction – restriction fragment length polymorphism) analysis. The PCR-RFLP was performed at Royal GD, the Netherlands. The (partial) sequencing of the US8 gene was performed using Sanger sequencing method. The phylogenetic analysis, using sequences of 2 Ukrainian strains (MZ323228, MZ333273) and 17 international gene sequences present in GenBank, was performed using the Maximum Likelihood method. For comparative analysis, sequences of vaccine ILT virus strains were used. Results. Over the years 2010-2019, 7 isolates of ILTV were obtained from sick and dead poultry with typical clinical signs and internal lesions at industrial and backyard farms of the Kharkiv, Donetsk, Luhansk and Sumy regions, and the Autonomous Republic of Crimea. Other avian respiratory viral and bacterial pathogens were not detected. Five isolates were obtained from poultry of industrial holdings where vaccination against ILT is carried out. Using PCR-RFLP analysis of 4 isolates, we found that three of them (Sumy 6-11/19, A 04-12, B 2-10) to belong to vaccine-type ILTV strains and only one, B 59-11strain, belongs to wild-type ILTV. Vaccine-type ILTV strains circulated and possibly still circulate in Ukraine in industrial and backyard poultry farms among both vaccinated and non- vaccinated poultry. An ILTV wild-type strain was obtained from non-vaccinated chickens from a backyard farm, which may indicate an important role of backyard farms in maintaining the circulation of the virus. After partial sequencing and phylogenetic analysis of the ILTV US8 gene the two Ukrainian strains studied were placed into two different clusters: The vaccine-type B 2-10 strain, obtained from sick vaccinated chickens from an industrial farm, was close to vaccine-type strains circulating in, China, Italy and the USA. The wild-type B 59-11strain,
{"title":"Molecular and genetic characterization of avian laryngotracheitis virus isolates obtained in Ukraine","authors":"A. Veretsun, B. Stegniy, O. Rula, V. Bolotin, A. Stegniy, A. Gerilovych, D. Muzyka","doi":"10.15407/agrisp8.01.032","DOIUrl":"https://doi.org/10.15407/agrisp8.01.032","url":null,"abstract":"Aim. To conduct a virological, PCR, PCR-RFLP and sequencing study of infectious laryngotracheitis virus (ILTV)\u0000isolates obtained from sick and dead chickens at industrial and backyard poultry farms in the eastern region of Ukraine\u0000collected over the years 2010–2019 and to establish their pathotype and relationship with internationally occurring strains.\u0000Methods. Material for virological studies was collected in the framework of research program of the NSC IEСVM during\u00002010-2019 in the poultry farms in the North-Eastern region of Ukraine, where the birds with the respiratory clinical\u0000signs were found. In total, 28 poultry farms were observed. ILTV isolates were obtained with conventional methods,\u0000using 10–12-day-old chicken embryos. A 0,2 ml of 10–20 % suspension of pathological material in PBS was used for\u0000inoculation. For in-depth studies, we used 4 isolates of ILTV obtained from sick and dead chickens from industrial\u0000and backyard poultry farms in Kharkiv, Luhansk, Donetsk, and Sumy regions from 2010–2019. The identification of\u0000ILTV isolates was performed via conventional PCR. The pathotype of ILTV strains was determined using PCR-RFLP\u0000(polymerase chain reaction – restriction fragment length polymorphism) analysis. The PCR-RFLP was performed at\u0000Royal GD, the Netherlands. The (partial) sequencing of the US8 gene was performed using Sanger sequencing method.\u0000The phylogenetic analysis, using sequences of 2 Ukrainian strains (MZ323228, MZ333273) and 17 international gene\u0000sequences present in GenBank, was performed using the Maximum Likelihood method. For comparative analysis,\u0000sequences of vaccine ILT virus strains were used. Results. Over the years 2010-2019, 7 isolates of ILTV were obtained\u0000from sick and dead poultry with typical clinical signs and internal lesions at industrial and backyard farms of the Kharkiv,\u0000Donetsk, Luhansk and Sumy regions, and the Autonomous Republic of Crimea. Other avian respiratory viral and bacterial\u0000pathogens were not detected. Five isolates were obtained from poultry of industrial holdings where vaccination against\u0000ILT is carried out. Using PCR-RFLP analysis of 4 isolates, we found that three of them (Sumy 6-11/19, A 04-12, B 2-10)\u0000to belong to vaccine-type ILTV strains and only one, B 59-11strain, belongs to wild-type ILTV. Vaccine-type ILTV strains\u0000circulated and possibly still circulate in Ukraine in industrial and backyard poultry farms among both vaccinated and non-\u0000vaccinated poultry. An ILTV wild-type strain was obtained from non-vaccinated chickens from a backyard farm, which\u0000may indicate an important role of backyard farms in maintaining the circulation of the virus. After partial sequencing and\u0000phylogenetic analysis of the ILTV US8 gene the two Ukrainian strains studied were placed into two different clusters: The\u0000vaccine-type B 2-10 strain, obtained from sick vaccinated chickens from an industrial farm, was close to vaccine-type\u0000strains circulating in, China, Italy and the USA. The wild-type B 59-11strain, ","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2021-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41735451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Гуральська, Кот, Дишлюк, Заїка, Хоменко, Героїв Оборони
Aim. To determine the difference in immune responses of the harderian gland in clinically healthy chickens and the ones with infectious bronchitis based on the content, localization and morphometric estimation of the surface markers of Т- and В-lymphocytes and to determine the differentiation index as an indicator of assessing body defenses. Methods. Histological, immunohistochemical, optical, morphometric and statistical. Results. The histological study of the harderian gland of chickens with infectious bronchitis determined the swelling and proliferation of the connective tissue as well as infiltration of secretory lobules by lymphoid cells. It was found that the immunity of chickens with infectious bronchitis, in which the harderian gland plays a relevant role, depends considerably on the differentiation index of immunocompetent cells. There was a reliable 1.77- and 1.36-fold decrease in this indicator for 40- and 90-day-old chickens, respectively, in case of nephroso-nephritic form of infectious bronchitis which demonstrated a weaker function of the defense cells of this organ. According to the cytomorphometric analysis, the number of cells, expressing CD4+, CD8+, CD20+, CD45RA+ markers in the harderian gland of sick 20-, 40-, and 90-day-old chickens with respiratory and nephroso-nephritic forms of infectious bronchitis was reliably (P < 0.05) increasing compared to the clinically healthy chickens. For instance, the number of mature В-lymphocytes increased in sick 20-day-old chickens – 2.44 times, 40-day-old chickens – 1.88 times, and 90-day-old ones – 2.62 times compared to clinically healthy chickens. Conclusions. The data were obtained about the changes in quantitative and qualitative composition of lymphocytes with surface markers CD4+, CD8+, CD20+, CD45RA+ in the harderian gland of chickens with infectious bronchitis. Our results will supplement current knowledge about the feasibility of immunohistochemical methods in the diagnostics of avian infectious bronchitis.
{"title":"Immune response of the harderian gland in chickens to infectious bronchitis coronavirus","authors":"Гуральська, Кот, Дишлюк, Заїка, Хоменко, Героїв Оборони","doi":"10.15407/agrisp8.01.049","DOIUrl":"https://doi.org/10.15407/agrisp8.01.049","url":null,"abstract":"Aim. To determine the difference in immune responses of the harderian gland in clinically healthy chickens and the\u0000ones with infectious bronchitis based on the content, localization and morphometric estimation of the surface markers\u0000of Т- and В-lymphocytes and to determine the differentiation index as an indicator of assessing body defenses.\u0000Methods. Histological, immunohistochemical, optical, morphometric and statistical. Results. The histological study\u0000of the harderian gland of chickens with infectious bronchitis determined the swelling and proliferation of the connective\u0000tissue as well as infiltration of secretory lobules by lymphoid cells. It was found that the immunity of chickens with\u0000infectious bronchitis, in which the harderian gland plays a relevant role, depends considerably on the differentiation\u0000index of immunocompetent cells. There was a reliable 1.77- and 1.36-fold decrease in this indicator for 40- and\u000090-day-old chickens, respectively, in case of nephroso-nephritic form of infectious bronchitis which demonstrated a\u0000weaker function of the defense cells of this organ. According to the cytomorphometric analysis, the number of cells,\u0000expressing CD4+, CD8+, CD20+, CD45RA+ markers in the harderian gland of sick 20-, 40-, and 90-day-old chickens\u0000with respiratory and nephroso-nephritic forms of infectious bronchitis was reliably (P < 0.05) increasing compared\u0000to the clinically healthy chickens. For instance, the number of mature В-lymphocytes increased in sick 20-day-old\u0000chickens – 2.44 times, 40-day-old chickens – 1.88 times, and 90-day-old ones – 2.62 times compared to clinically\u0000healthy chickens. Conclusions. The data were obtained about the changes in quantitative and qualitative composition\u0000of lymphocytes with surface markers CD4+, CD8+, CD20+, CD45RA+ in the harderian gland of chickens with infectious\u0000bronchitis. Our results will supplement current knowledge about the feasibility of immunohistochemical methods in\u0000the diagnostics of avian infectious bronchitis.","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2021-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49599659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Dubrovna, G. Priadkina, S. Mykhalska, A. Komisarenko
Aim. To determine water deficiency tolerance of genetically modified common wheat plants (Triticum aestivum L., cv Zymoyarka), containing the heterologous ornithine-δ-aminotransferase gene, based on the analysis of grain productivity and physiological and biochemical characteristics in transgenic and non-transgenic genotypes. Methods. Biochemical spectrophotometric assays: the enzyme ornithine-δ-aminotransferase activity, the free L-proline content, and the photosynthetic pigments content; biotechnological: Agrobacterium-mediated transformation in planta; physiological: morphometric traits and elements of grain productivity; mathematical statistics. Results. It was established that the presence of an additional copy of the ornithine-δ-aminotransferase gene in transgenic plants leads to higher activity of the ornithine-δ- aminotransferase enzyme: by 1.6 times higher on average for all lines as compared to the non-transgenic plants at 70 % of fi eld capacity and by 1.5 – at 30 % fi eld capacity. However, transgenic plants did not differ significantly from the original variety in the free L-proline content either under optimal water conditions or under soil drought. The increase in the total chlorophyll (a + b) content in flag leaves of transgenic plants was established under conditions of both optimal water supply and drought, as compared with the original genotype (increase by 5–7 % and 8–11 %, respectively). The enhanced expression of the orni- thine-δ-aminotransferase gene in the transgenic plants stimulated root growth both under optimal and stressful conditions: the root length of the transformed plants exceeded that of the original variety by 3.4–3.9 cm in the variant with optimal water supply, and by 4.2–4.6 cm – under drought. They were also characterized by a more developed root system. Dry root weight of the transgenic plants exceeded the original variety both in the control (by 23–27 %), and under drought (by 37– 44 %). Under drought, the root dry weight decreased by 29 % in the plants of the original variety, compared 70 % fi eld capacity, and by 11–15 % in the lines. Under 30 % field capacity, the transgenic lines also exceeded non-transformed plants in the number of grains from the whole plant (on average for 3 lines by 26 %) and in the grain weight (by 22 %). Transgenic plants are characterized by the formation of a higher productive shoots number: from 3.2 to 3.4 compared with 2.5 in non-transgenic plants at 70 % fi eld capacity and 2.7–3.1 vs 2.2 at 30 % field capacity it was found. Conclusions. Thus, the analysis of genetically modified common wheat plants cv. Zymoyarka, containing the heterologous alfalfa ornithine-δ- aminotransferase gene, by yield structure elements, morphometric parameters and photosynthetic pigment content showed their better tolerance to soil drought as compared to non-transgenic plants. We explain the improvement of grain productivity of the whole plant in transgenic wheat lines with an additional copy of o
{"title":"Water deficiency tolerance of genetically modified common wheat cv. Zymoyarka, containing a heterologous ornithine-δ-aminotransferase gene","authors":"O. Dubrovna, G. Priadkina, S. Mykhalska, A. Komisarenko","doi":"10.15407/agrisp8.01.014","DOIUrl":"https://doi.org/10.15407/agrisp8.01.014","url":null,"abstract":"Aim. To determine water deficiency tolerance of genetically modified common wheat plants (Triticum aestivum L., cv\u0000Zymoyarka), containing the heterologous ornithine-δ-aminotransferase gene, based on the analysis of grain productivity\u0000and physiological and biochemical characteristics in transgenic and non-transgenic genotypes. Methods. Biochemical\u0000spectrophotometric assays: the enzyme ornithine-δ-aminotransferase activity, the free L-proline content, and the\u0000photosynthetic pigments content; biotechnological: Agrobacterium-mediated transformation in planta; physiological:\u0000morphometric traits and elements of grain productivity; mathematical statistics. Results. It was established that the presence\u0000of an additional copy of the ornithine-δ-aminotransferase gene in transgenic plants leads to higher activity of the ornithine-δ-\u0000aminotransferase enzyme: by 1.6 times higher on average for all lines as compared to the non-transgenic plants at 70 % of fi eld\u0000capacity and by 1.5 – at 30 % fi eld capacity. However, transgenic plants did not differ significantly from the original variety\u0000in the free L-proline content either under optimal water conditions or under soil drought. The increase in the total chlorophyll\u0000(a + b) content in flag leaves of transgenic plants was established under conditions of both optimal water supply and drought,\u0000as compared with the original genotype (increase by 5–7 % and 8–11 %, respectively). The enhanced expression of the orni-\u0000thine-δ-aminotransferase gene in the transgenic plants stimulated root growth both under optimal and stressful conditions:\u0000the root length of the transformed plants exceeded that of the original variety by 3.4–3.9 cm in the variant with optimal\u0000water supply, and by 4.2–4.6 cm – under drought. They were also characterized by a more developed root system. Dry root\u0000weight of the transgenic plants exceeded the original variety both in the control (by 23–27 %), and under drought (by 37–\u000044 %). Under drought, the root dry weight decreased by 29 % in the plants of the original variety, compared 70 % fi eld\u0000capacity, and by 11–15 % in the lines. Under 30 % field capacity, the transgenic lines also exceeded non-transformed plants\u0000in the number of grains from the whole plant (on average for 3 lines by 26 %) and in the grain weight (by 22 %). Transgenic\u0000plants are characterized by the formation of a higher productive shoots number: from 3.2 to 3.4 compared with 2.5 in\u0000non-transgenic plants at 70 % fi eld capacity and 2.7–3.1 vs 2.2 at 30 % field capacity it was found. Conclusions. Thus,\u0000the analysis of genetically modified common wheat plants cv. Zymoyarka, containing the heterologous alfalfa ornithine-δ-\u0000aminotransferase gene, by yield structure elements, morphometric parameters and photosynthetic pigment content showed\u0000their better tolerance to soil drought as compared to non-transgenic plants. We explain the improvement of grain productivity\u0000of the whole plant in transgenic wheat lines with an additional copy of o","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2021-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46196745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. B. Kovalev, T. I. Kozlik, L. Protsenko, A. Bober, B. F. Kormiltsev
Despite the decline in the national hop production, a part of hop products, made of Ukrainian aroma hop, is highly estimated in the international market and exported. Therefore, a relevant prerequisite of ensuring a suffi cient level of competitiveness of domestic hop production is the expansion of its assortment. To satisfy this need the breeders face the task of creating varieties with unique characteristics, which requires searching for new forms and strains of hop with increased content of different biologically active compounds in cones. In this regard national research program aimed to investigate genetic stab ility of hop varieties during multiple planting and storing of explants in in vitro conditions while introducing them into the collection, adapting the composition of Murashige and Skoog culture medium for specifi c varieties is discussed. Studies conducted included the analysis of plants by morphological and variety-specifi c traits, the identifi cation of varieties by biochemical criteria, improvement of hop regenerants using ELISA, molecular-genetic analysis based on PCR (polymerase chain reaction) for identifi cation of hop genotypes and determination of genetic stability, and the improvement of method of microclonal reproduction of hop. As a result of the perennial research of cultivating hop planting material using the Murashige and Skoog complex of nutrients, the foundations of creating and maintaining the in vitro collection of hop varieties were fi rst elaborated in Ukraine which allow for the possibility of decreasing the concentration of nutrients in the culture medium by 50 %, replacing expensive gel-forming preparation for the maintenance of plants in the culture medium - agar-agar, the share of whose cost in the medium composition is up to 70 %, with a cheaper substance - modifi ed starch DDKamod or agroperlite, and reducing the expenses for the maintenance of genetic pool in the in vitro collection. It was determined that the spectra of microsatellite loci of the amplifi ed DNA of the explants, cultivated in vitro, during the study period did not differ from the spectra of plant DNA prior to cloning, which demonstrated DNA stability and allowed cultivating hop varieties in the in vitro culture without any changes in the genome. The composition of media for cultivation and long- term storing of hop varieties in in vitro conditions was selected.
{"title":"Extending and maintaining the in vitro collection of (inter)national hop varieties in Ukraine","authors":"V. B. Kovalev, T. I. Kozlik, L. Protsenko, A. Bober, B. F. Kormiltsev","doi":"10.15407/AGRISP7.03.061","DOIUrl":"https://doi.org/10.15407/AGRISP7.03.061","url":null,"abstract":"Despite the decline in the national hop production, a part of hop products, made of Ukrainian aroma hop, is highly\u0000estimated in the international market and exported. Therefore, a relevant prerequisite of ensuring a suffi cient level\u0000of competitiveness of domestic hop production is the expansion of its assortment. To satisfy this need the breeders\u0000face the task of creating varieties with unique characteristics, which requires searching for new forms and strains\u0000of hop with increased content of different biologically active compounds in cones. In this regard national research\u0000program aimed to investigate genetic stab ility of hop varieties during multiple planting and storing of explants in in\u0000vitro conditions while introducing them into the collection, adapting the composition of Murashige and Skoog culture\u0000medium for specifi c varieties is discussed. Studies conducted included the analysis of plants by morphological and\u0000variety-specifi c traits, the identifi cation of varieties by biochemical criteria, improvement of hop regenerants using\u0000ELISA, molecular-genetic analysis based on PCR (polymerase chain reaction) for identifi cation of hop genotypes and\u0000determination of genetic stability, and the improvement of method of microclonal reproduction of hop. As a result\u0000of the perennial research of cultivating hop planting material using the Murashige and Skoog complex of nutrients,\u0000the foundations of creating and maintaining the in vitro collection of hop varieties were fi rst elaborated in Ukraine\u0000which allow for the possibility of decreasing the concentration of nutrients in the culture medium by 50 %, replacing\u0000expensive gel-forming preparation for the maintenance of plants in the culture medium - agar-agar, the share of whose\u0000cost in the medium composition is up to 70 %, with a cheaper substance - modifi ed starch DDKamod or agroperlite,\u0000and reducing the expenses for the maintenance of genetic pool in the in vitro collection. It was determined that the\u0000spectra of microsatellite loci of the amplifi ed DNA of the explants, cultivated in vitro, during the study period did not\u0000differ from the spectra of plant DNA prior to cloning, which demonstrated DNA stability and allowed cultivating hop\u0000varieties in the in vitro culture without any changes in the genome. The composition of media for cultivation and long-\u0000term storing of hop varieties in in vitro conditions was selected.","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49250619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. V. Biloivan, B. Stegniy, A. Gerilovych, V. Arefiev, R. Wölfel, J. Schwarz, C. Popp, G. Grass
Aim. The aim of this study was to screen soil samples of 17 anthrax burial sites in Eastern and Southern Ukraine for the presence of B. anthracis. Methods. Soil samples were collected from anthrax grave sites located in Kharkiv, Sumy and Mykolaiv regions (diseased animals dated from 1946 to 2003). Isolation of B. anthracis from collected soil samples was performed with the GABRI method. From single colonies without hemolysis, that were inactivated with peracetic acid- containing 2 % Terralin PAA solution, DNA was extracted and analyzed by qPCR for the presence of chromosomal marker dhp61, as well as the markers pagA and capC located on virulence plasmids pXO1 and pXO2, respectively. Results. Eleven fi eld trips were conducted from July, 2016 to October, 2018 in which 369 soil samples from 17 burial sites in Kharkiv, Sumy and Mykolaiv oblasts were collected from different depths of presumed anthrax carcass sites. In most cases (12 out of 17 cases), the current status of these burial sites was deteriorated and not prop- erly accounted for. It was possible to obrain viable B. anthracis isolate was obtained from 50 cm depth at the grave site near Koviagy village, Valky district, Kharkiv region (49.92373°N, 35.48951°E). This isolate was named KhR/ VD/Kov2-2-05-3 and deposited in the Collection of Animal Infectious Pathogens of the National Scientifi c Center “Institute of Experimental and Clinical Veterinary Medicine”, Kharkiv, Ukraine. The contamination level of soil at the isolation site reached about 10 4 CFU per g as determined by plate counting. qPCR analysis of this isolate identi- fi ed both the dhp61 B. anthracis chromosomal and the pagA virulence plasmid marker. However, the plasmid pXO2 marker, required for capsule-formation could not be detected. Conclusions. The anthrax burial sites were created between the 1920s and 1960s, however, only approximate locations could be found and demarcated. In most cases the status of the sites was unsuitable for sampling. Nevertheless, isolation of B. anthracis in one case in the Valky district shows that old anthrax burial sites (13.500 exist in Ukraine) still pose a risk as potential source of the infection and therefore require more attention and surveillance, for which a surveillance plan will be developed.
{"title":"Screening of possibly anthrax-contaminated burial sites in eastern and southern Ukraine","authors":"O. V. Biloivan, B. Stegniy, A. Gerilovych, V. Arefiev, R. Wölfel, J. Schwarz, C. Popp, G. Grass","doi":"10.15407/AGRISP7.03.003","DOIUrl":"https://doi.org/10.15407/AGRISP7.03.003","url":null,"abstract":"Aim. The aim of this study was to screen soil samples of 17 anthrax burial sites in Eastern and Southern Ukraine\u0000for the presence of B. anthracis. Methods. Soil samples were collected from anthrax grave sites located in Kharkiv,\u0000Sumy and Mykolaiv regions (diseased animals dated from 1946 to 2003). Isolation of B. anthracis from collected soil\u0000samples was performed with the GABRI method. From single colonies without hemolysis, that were inactivated with\u0000peracetic acid- containing 2 % Terralin PAA solution, DNA was extracted and analyzed by qPCR for the presence of\u0000chromosomal marker dhp61, as well as the markers pagA and capC located on virulence plasmids pXO1 and pXO2,\u0000respectively. Results. Eleven fi eld trips were conducted from July, 2016 to October, 2018 in which 369 soil samples\u0000from 17 burial sites in Kharkiv, Sumy and Mykolaiv oblasts were collected from different depths of presumed anthrax\u0000carcass sites. In most cases (12 out of 17 cases), the current status of these burial sites was deteriorated and not prop-\u0000erly accounted for. It was possible to obrain viable B. anthracis isolate was obtained from 50 cm depth at the grave\u0000site near Koviagy village, Valky district, Kharkiv region (49.92373°N, 35.48951°E). This isolate was named KhR/\u0000VD/Kov2-2-05-3 and deposited in the Collection of Animal Infectious Pathogens of the National Scientifi c Center\u0000“Institute of Experimental and Clinical Veterinary Medicine”, Kharkiv, Ukraine. The contamination level of soil at\u0000the isolation site reached about 10 4 CFU per g as determined by plate counting. qPCR analysis of this isolate identi-\u0000fi ed both the dhp61 B. anthracis chromosomal and the pagA virulence plasmid marker. However, the plasmid pXO2\u0000marker, required for capsule-formation could not be detected. Conclusions. The anthrax burial sites were created\u0000between the 1920s and 1960s, however, only approximate locations could be found and demarcated. In most cases the\u0000status of the sites was unsuitable for sampling. Nevertheless, isolation of B. anthracis in one case in the Valky district\u0000shows that old anthrax burial sites (13.500 exist in Ukraine) still pose a risk as potential source of the infection and\u0000therefore require more attention and surveillance, for which a surveillance plan will be developed.","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44363439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Tucak, T. Čupić, D. Horvat, G. Krizmanić, M. Ravlić
Aim. Alfalfa is a rich source of phytoestrogens, among them coumestrol which shows strong estrogenic activity that can adversely affect the health of domestic animals. The aim of the study was to determine the variation in coumestrol content in leaves of alfalfa breeding populations, present in the breeding program of Agricultural Institute Osijek in Croatia. Method. Twelve alfalfa populations were selected based on their high forage yield and good persistence. Coumestrol was extracted using acidifi ed methanol as an organic solvent from lyophilized and ground alfalfa leaves, while for detection and quantifi cation was used. Results. Signifi cant differences were observed between the studied populations with average coumestrol content of 435.67 mg/kg of dry matter (DM). The highest content of coumestrol was determined in breeding population Rs-21 (619.53 mg/kg of DM). Conclusions. Populations Rs-33 and Rs-20 had the lowest coumestrol content (82.18 and 86.58 mg/kg, respectively) and present a potential breeding source for creat- ing new contemporary cultivars with decreased coumestrol content
{"title":"Coumestrol content in alfalfabreeding populations","authors":"M. Tucak, T. Čupić, D. Horvat, G. Krizmanić, M. Ravlić","doi":"10.15407/AGRISP7.03.025","DOIUrl":"https://doi.org/10.15407/AGRISP7.03.025","url":null,"abstract":"Aim. Alfalfa is a rich source of phytoestrogens, among them coumestrol which shows strong estrogenic activity that\u0000can adversely affect the health of domestic animals. The aim of the study was to determine the variation in coumestrol\u0000content in leaves of alfalfa breeding populations, present in the breeding program of Agricultural Institute Osijek in\u0000Croatia. Method. Twelve alfalfa populations were selected based on their high forage yield and good persistence.\u0000Coumestrol was extracted using acidifi ed methanol as an organic solvent from lyophilized and ground alfalfa leaves,\u0000while for detection and quantifi cation was used. Results. Signifi cant differences were observed between the studied\u0000populations with average coumestrol content of 435.67 mg/kg of dry matter (DM). The highest content of coumestrol\u0000was determined in breeding population Rs-21 (619.53 mg/kg of DM). Conclusions. Populations Rs-33 and Rs-20 had\u0000the lowest coumestrol content (82.18 and 86.58 mg/kg, respectively) and present a potential breeding source for creat-\u0000ing new contemporary cultivars with decreased coumestrol content","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45330441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
One of the main factors of increasing the productivity of agricultural plants is intensifying the activity of the photo- synthetic apparatus, since the productivity of crops depends on the ability to absorb solar radiation and convert it into the energy of chemical bonds for further use in metabolic processes. The amount of photosynthetically active radia- tion absorbed by crops, in its turn, depends on the area, duration, and activity of the assimilation surface functioning. The use of fertilizers, that contain trace elements, can further achieve both raising the yield of cultivated plants and environmental protection. In this regard, the state-of-the-art research on the infl uence of the main trace elements (iron, zinc, manganese, molybdenum, cobalt, selenium, boron, titanium) and one macroelement - magnesium - on photosynthetic characteristics of plants and crops is discussed. Based on the literature data and the results of our own research, we documented the effect of trace elements on leaves carbon dioxide exchange rates, the content of photo- synthetic pigments, the antioxidant enzymes activity, as well as the traits of the photosynthetic apparatus capacity. The infl uence of nanometals on the content and ratio of pigments, net CO 2 assimilation rate, and the photochemical activity of photosystems, including the effect of stress factors, is discussed. The specifi cities of the infl uence of nanometals are discussed and possible mechanisms of the effect of low concentrations of trace elements on plant metabolism are analyzed. It is shown that trace elements infl uence photosynthetic processes in plants and the systems of their antioxi- dant protection. The relevance of trace elements in the development of new strategies to elaborate the technologies of cultivating next-generation plants, including those that will be based on new physical and chemical properties of macro- and micronutrients in a nano form, is highlighted
{"title":"Influence of trace elements, applied in classical and nano forms, on photosynthesis of higher plants in relation to enhancement of crop productivity","authors":"G. Priadkina","doi":"10.15407/AGRISP7.03.071","DOIUrl":"https://doi.org/10.15407/AGRISP7.03.071","url":null,"abstract":"One of the main factors of increasing the productivity of agricultural plants is intensifying the activity of the photo-\u0000synthetic apparatus, since the productivity of crops depends on the ability to absorb solar radiation and convert it into\u0000the energy of chemical bonds for further use in metabolic processes. The amount of photosynthetically active radia-\u0000tion absorbed by crops, in its turn, depends on the area, duration, and activity of the assimilation surface functioning.\u0000The use of fertilizers, that contain trace elements, can further achieve both raising the yield of cultivated plants and\u0000environmental protection. In this regard, the state-of-the-art research on the infl uence of the main trace elements\u0000(iron, zinc, manganese, molybdenum, cobalt, selenium, boron, titanium) and one macroelement - magnesium - on\u0000photosynthetic characteristics of plants and crops is discussed. Based on the literature data and the results of our own\u0000research, we documented the effect of trace elements on leaves carbon dioxide exchange rates, the content of photo-\u0000synthetic pigments, the antioxidant enzymes activity, as well as the traits of the photosynthetic apparatus capacity. The\u0000infl uence of nanometals on the content and ratio of pigments, net CO 2 assimilation rate, and the photochemical activity\u0000of photosystems, including the effect of stress factors, is discussed. The specifi cities of the infl uence of nanometals\u0000are discussed and possible mechanisms of the effect of low concentrations of trace elements on plant metabolism are\u0000analyzed. It is shown that trace elements infl uence photosynthetic processes in plants and the systems of their antioxi-\u0000dant protection. The relevance of trace elements in the development of new strategies to elaborate the technologies\u0000of cultivating next-generation plants, including those that will be based on new physical and chemical properties of\u0000macro- and micronutrients in a nano form, is highlighted","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46322380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. To study the effect of different concentrations of ethylene glycol and glycerin in equilibration and vitrifi cation solutions on 1) the viability and further development of frozen-thawed bovine ovarian cumulus-oocyte complexes (COCs), cryopreserved by vitrifi cation method, 2) on the effectiveness of inseminating mature oocytes, obtained from them, and 3) on the formation of embryos. Methods. Biotechnological, cryobiological, morphological, cytogenetic, and statistical methods, as well as methods of statistical data processing were used in the research. Results. The results of experimental studies on the effect of different concentrations of ethylene glycol and glycerin in the equilibration solution during cryopreservation of bovine ovarian COCs (n = 502) on their viability and further development after freezing-thawing are presented. We also show the results of the comparative analysis of cryoresistant properties of bovine ovarian COCs (n = 560) using different concentrations of ethylene glycol and glycerin, as cryoprotectants, in the vitrifi cation solution in terms of the viability and maturation of the oocytes, which originated from these COCs, up to metaphase II of meiosis. A comparative analysis of the application of ethylene glycol and glycerol in differ- ent concentrations for the equilibration and vitrifi cation solutions in cryopreserving bovine ovarian COCs (n = 220) demonstrated a relationship between the level of concentration of these cryoprotectants and the number of embryos after in vitro insemination of mature gametes, obtained from these frozen-thawed COCs. Conclusions. It was found that the use of 25 % ethylene glycol and 5 % glycerin in the equilibration solution and 10 % ethylene glycol and 40 % glycerin in the vitrifi cation solution during cryopreservation of bovine ovarian COCs ensures lower toxicity of these solutions and promotes more effi cient (up to 14.3 %) formation and development of embryos after in vitro insemina- tion of mature gametes, obtained from these COCs
{"title":"Biotechnological approaches to the preservation and use of bovine ovarian cumulus-oocyte complexes in the system of reproductive technologies","authors":"P. A. Trotskyi, O. Shcherbak, I. M. Lyuta","doi":"10.15407/AGRISP7.03.054","DOIUrl":"https://doi.org/10.15407/AGRISP7.03.054","url":null,"abstract":"Aim. To study the effect of different concentrations of ethylene glycol and glycerin in equilibration and vitrifi cation\u0000solutions on 1) the viability and further development of frozen-thawed bovine ovarian cumulus-oocyte complexes\u0000(COCs), cryopreserved by vitrifi cation method, 2) on the effectiveness of inseminating mature oocytes, obtained from\u0000them, and 3) on the formation of embryos. Methods. Biotechnological, cryobiological, morphological, cytogenetic,\u0000and statistical methods, as well as methods of statistical data processing were used in the research. Results. The results\u0000of experimental studies on the effect of different concentrations of ethylene glycol and glycerin in the equilibration\u0000solution during cryopreservation of bovine ovarian COCs (n = 502) on their viability and further development after\u0000freezing-thawing are presented. We also show the results of the comparative analysis of cryoresistant properties of\u0000bovine ovarian COCs (n = 560) using different concentrations of ethylene glycol and glycerin, as cryoprotectants, in\u0000the vitrifi cation solution in terms of the viability and maturation of the oocytes, which originated from these COCs,\u0000up to metaphase II of meiosis. A comparative analysis of the application of ethylene glycol and glycerol in differ-\u0000ent concentrations for the equilibration and vitrifi cation solutions in cryopreserving bovine ovarian COCs (n = 220)\u0000demonstrated a relationship between the level of concentration of these cryoprotectants and the number of embryos\u0000after in vitro insemination of mature gametes, obtained from these frozen-thawed COCs. Conclusions. It was found\u0000that the use of 25 % ethylene glycol and 5 % glycerin in the equilibration solution and 10 % ethylene glycol and 40 %\u0000glycerin in the vitrifi cation solution during cryopreservation of bovine ovarian COCs ensures lower toxicity of these\u0000solutions and promotes more effi cient (up to 14.3 %) formation and development of embryos after in vitro insemina-\u0000tion of mature gametes, obtained from these COCs","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43515858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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