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Impact of radioactive contamination of soils on the diversity of micropopulation and the transformation of organic substances 土壤放射性污染对微生物种群多样性和有机物转化的影响
IF 0.4 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-04-20 DOI: 10.15407/agrisp9.03.003
I. Gudkov, I. Volkohon, V. Illienko, M. Lazarev, A. Klepko
Aim. To study the impact of different levels of radioactive contamination on the organic matter decomposition andthe population development of microbial decomposers of organic matter in soil. Methods. Gamma-spectrometryfor the determination of the relative activity of 137Cs and beta-spectrometry for the determination of the relativeactivity of 90Sr in order to choose the contamination range for the studies; Tea Bag Іndex (TBI), the standard glob-ally accepted method to determine the rate of organic matter decomposition; gas chromatography – to determinethe impact of the investigated factors on the formation of the biomass of microorganisms by means of the СО2production potential; classic microbiological methods, using elective media to estimate the population densitiesof culturable microbial decomposers. Results. The studies (from April to September 2021) comprised two soilswith different radioactive contamination ranges: Range No. 1 in Narodychi district of Zhytomyr region (the villageKhrystynivka, 3 sampling points) in the unconditional (obligatory) resettlement zone after the catastrophe in theChornobyl nuclear power plant (ChNPP) (it has an absorbed dose rate gradient, evaluated for soil microorganisms,from 0.2 μGy/h (at sampling point Narodychi-1: 51,24076°N, 29,21497°E) to 1.57 μGy/h (at sampling point Naro-dychi-3: 51,23815°N, 29,22245°E)), located on the agricultural field, abandoned after the catastrophe and RangeNo. 2, located on the afforested area, directly bordering the territory of the so-called Red Forest in the ChNPP ex-clusion zone (4 sampling points – the first three points – natural ecosystems, and 4th – pyrogenically transformedterritory after fires in 2020) with the absorbed dose rate gradient from 3.7 μGy/h (at sampling point ChEZ-1:51,38595°N, 30,03035°E) to 84.0 μGy/h (at sampling point ChEZ-4: 51,38231°N, 30,03298°E). The dependenceof the studied indicators on the gradient of soil contamination with radionuclides was demonstrated and these arethe conditions that the difference is more than 400 times in the values of the ionizing radiation absorbed dose ratesformed by radionuclides in soil. In Range No. 1 (on the fallow land), with the increase in the dose rate up from 0.2to 1.57 μGy/h, there was statistically significant increase of mineralization coefficient and percentage of reducedweight of rooibos tea between the least and most contaminated sampling points), stimulation of the developmentof micromycetes (by 1.2–2.3 times), cellulose-decomposing bacteria (by 7.1–7.9 times), and ammonifying bacteria(by 1.8–6.3 times) due to a significant increasing the number of colony-forming units in points with higher radio-nuclide contamination and an increase of microbial biomass in soil more than twofold between the least and mostcontaminated sampling point of Range No. 1.In Range No. 2, in the ChNPP exclusion zone, characterized by lowparameters of sod-podzolic soil fertility (humus content from 1.2 ± 0.
目标研究不同水平的放射性污染对土壤中有机物分解和微生物分解者种群发育的影响。方法。γ光谱法测定137Cs的相对活性,β光谱法测定90Sr的相对活性以选择研究的污染范围;袋泡茶(TBI),全球公认的测定有机物分解速率的标准方法;气相色谱法-通过СО2生产潜力测定所研究因素对微生物生物量形成的影响;经典微生物学方法,使用选择性培养基来估计可培养微生物分解者的种群密度。后果这些研究(2021年4月至9月)包括两个放射性污染范围不同的土壤:切尔诺贝利核电站(ChNPP)灾难后,无条件(强制性)安置区内Zhytomyr地区Narodychi区的1号土壤(Khrystynivka村,3个采样点)(土壤微生物的吸收剂量率梯度为0.2μGy/h(采样点Narodychi-1:5124076°N,2921497°E)至1.57μGy/h。2、位于绿化区,直接与ChNPP隔离区内所谓的红森林接壤(4个采样点——前三个点——自然生态系统,第四个——2020年火灾后热解转化的生态系统),吸收剂量率梯度从3.7μGy/h(采样点ChEZ-1:5138595°N,3003035°E)到84.0μGy/h(采样点ChEZ-4:5138231°N,3003298°E)。证明了所研究的指标与放射性核素污染土壤的梯度的相关性,即放射性核素在土壤中的电离辐射吸收剂量率值相差400倍以上的条件。在1号范围(休耕地),随着剂量率从0.2μGy/h增加到1.57μGy/h,在污染程度最低和污染程度最高的采样点之间,卢比波斯茶的矿化系数和还原重量百分比有统计学意义的增加),刺激了微菌的发育(增加了1.2-2.3倍),纤维素分解细菌(增加了7.1-7.9倍),和氨化细菌(增加了1.8–6.3倍),这是因为在放射性核素污染程度较高的采样点,菌落形成单位的数量显著增加,土壤中微生物生物量在1号范围的最少和最多接触采样点之间增加了两倍多。在2号范围,在ChNPP禁区内,土壤腐殖质含量在1.2±0.2~0.9±0.1%之间,交换性酸度在4.0±0.1~5.0±0.7之间,土壤中的微生物和氨化细菌数量比1号范围低一到两个数量级。在纤维素分解微生物群中,微菌占主导地位。RangeNo的ChEZ-1、ChEZ-2和ChEZ-3前三个地块的矿化系数和rooibos茶的减重百分比随着放射性核素污染水平的增加而显著增加。2.结论。在无条件(强制性)安置区的休耕地,土壤中的辐射吸收剂量率从0.2μGy/h增加到1.57μGy/h,这并不能抑制土壤中微小器官的发育,而是刺激了它们的活动并增加了它们的数量。在切尔诺贝利核电站隔离区贫瘠的草皮-灰化土上,微菌的开发优势明显超过细菌-纤维素酶。放射性污染不仅在文献中描述的切尔诺贝利灾难后的最初几年影响了土壤微生物的发育和土壤中的生物过程,而且在相当长的一段时间内(事故发生后的30多年)都受到了影响。在有机植物残留物分解的糖分解模式的微生物中,微菌占主导地位。
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引用次数: 0
Symbiotic interaction between a mixture of Bradyrhizobium japonicum strains and different soybean cultivars 缓生根瘤菌株系与不同大豆品种杂交的共生互作
IF 0.4 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-04-20 DOI: 10.15407/agrisp9.03.036
D. Krutylo
Aim. To study the mutual relations between different soybean cultivars and two Bradyrhizobium japonicum strainsafter mixed inoculation, to evaluate the impact of the introduced microorganisms on the local rhizobial communitiesin soil, the formation and functioning symbiotic systems, and productivity of soybean. Methods. Microbiological andserological methods; field experiment, gas chromatography and mathematical-statistical methods. Results. A small-plot field experiment demonstrated that the use of a mixture of B. japonicum 46 and B. japonicum КВ11 strains pro-moted a more even distribution of the local and introduced rhizobia in the nodule populations of 12 soybean cultivarsof different geographic origin. There was no domination of particular strains in the nodules, and the symbiotic systemsformed were more balanced than the control without inoculation. After inoculation of the above-mentioned mixture,in most cultivars there was a considerable increase in the number of nodules (10–45 % more) and their mass (11–86% increase). There was also an increase in the level of symbiotic nitrogen fixation with 1.2–4.2 times and an increasein the weight of grain per plant by 6–29 % (depending on the cultivar). The efficiency of a mixture of B. japonicum 46and КВ11 strains in a preparation Rizogumin was confirmed in extensive field trials with soybean on an area of about60 thousand ha in different regions of Ukraine. In the background of the local populations of the soybean nodulatingbacteria in the areas, the application of the formulated mixture of the two rhizobia strains ensured a stable increase by15–33 % in the soybean yield as compared with the control without the inoculation. Conclusions. A novel approachwas investigated, which lies in the application of a mixture of B. japonicum 46 (genetic group USDA 6) and B. japoni-cum КВ11 (genetic group USDA 123) strains for different soybean cultivars inoculation. Combining these two strainsand their introduction into agrocenoses ensured the formation of a balanced symbiotic systems (without clear domina-tion of some strains in the nodules). This was expressed in an intensified nodulation, symbiotic nitrogen fixation, andincreased yield of different cultivars of soybeans by 15–33 % as compared to the control (without inoculation).
的目标。研究不同大豆品种与两种缓生根瘤菌混合接种后的相互关系,评价引入微生物对当地土壤根瘤菌群落、共生系统的形成和功能以及大豆生产力的影响。方法。微生物学和血清学方法;现场实验、气相色谱和数理统计方法。结果。小块田间试验表明,在不同地理来源的12个大豆品种的根瘤菌群体中,使用日本芽孢杆菌46和日本芽孢杆菌КВ11菌株混合施用,本地根瘤菌和引进根瘤菌的分布更加均匀。结核中没有特定菌株的优势,形成的共生系统比未接种的对照更平衡。接种上述混合物后,大多数品种的根瘤数量和质量均显著增加(增加10 - 45%)和11-86%。共生固氮水平提高1.2 ~ 4.2倍,单株粒重提高6 ~ 29%(依品种而定)。在乌克兰不同地区约6万公顷的大豆上进行了广泛的田间试验,证实了日本芽孢杆菌46和КВ11菌株混合制剂Rizogumin的效率。在该地区大豆根瘤菌当地种群的背景下,施用两种根瘤菌配制的混合物,与不接种的对照相比,大豆产量稳定提高了15 - 33%。结论。采用遗传群USDA - 6的日本芽孢杆菌46和遗传群USDA - 123的日本芽孢杆菌КВ11混合菌株接种不同大豆品种。结合这两种菌株并将它们引入农合菌确保了平衡共生系统的形成(在根瘤中没有明显的优势菌株)。这表现在结瘤增强,共生固氮,不同品种的大豆产量比对照(未接种)增加15 - 33%。
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引用次数: 0
The effect of inoculation with Azospirillum brasilense strain 410 on spring barley cv. nosivsky development and yield 接种巴西氮螺旋菌410株对春大麦cv的影响。诺西夫斯基发展和产量
IF 0.4 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-04-20 DOI: 10.15407/agrisp9.03.064
V. Volkogon, A. Moskalenko, S. Dimova, K. Volkogon, L. Potapienko
Aim. To study the efficiency of inoculating spring barley with Azospirillum brasilense 410. Methods. 1) A field ex-periment on turfpodzolic sandy soil with different mineral fertilization regimes; 2) A greenhouse experiment on sandas substrate, with isotope dilution analysis using 15N; 3) A lysimetric experiment in a stationary lysimetric installation.Furthermore, chromatography to determine nitrogenase activity of bacteria in the root zone of plants, agrochemical,and statistical methods. Results. Under field conditions, the inoculation with A. brasilense 410 promoted a significantincrease (37–103 %) in the nitrogenase activity in the “soil-plant” system without any mineral fertilization and (espe-cially) where N60P60K60 was used. A high fertilizer level (N120P120K120) lead to a long-term inhibition of the nitrogenaseactivity. In both cases (plants with and without inoculation with A. brasilense 410) this fertilization level showed anincrease in the nitrogenase activity only at the end of the vegetation period. The highest increase in yield (0.7 t/ha,27 %) in yield following A. brasilense strain 410 inoculation, occurred in plots with N60P60K60 fertilization; the leastincrease in yield (0.33 t/ha, 16.5 %) was observed in plots receiving no fertilizers. The pre-sowing inoculation ledto an increase in the protein content of 0.3–1.0 % in the barley grain, especially when receiving high fertilizationlevels, enhancing its value for the use in cereals and feeds, but decreasing its value for its use in brewing. The green-house experiment with 15N established an increase 77.1 % in the nitrogen intake into the plants due to the activationof the nitrogen-fixation process and enhanced 29.5 % nitrogen consumption from fertilizers. The lysimetric studiesdemonstrated that inoculation of spring barley cv Nosivsky with A. brasilense 410 limited the vertical migration andleaching of nitrogen by 27–30 %, potassium by 13–30, calcium by 32–51 %, manganese by 33–100 %, and water-sol-uble organic matter by 46–75 %. Conclusions. The pre-sowing inoculation of spring barley cv. Nosivsky seeds withA. brasilense 410 intensifies nitrogen consumption by plants within 29.5 % due to active nitrogen-fixation and a betterutilization of N from mineral fertilizers. The barley yield increase with 0.7 t/ha was in our limited experiment, usingone cultivar roughly equivalent to the increase after mineral fertilization with N60P60K60. Thus, pre-sowing inoculationwith A. brasilense 410 may lead to substantial reduction of the use of mineral fertilisers in practice.
的目标。研究巴西氮螺旋菌410接种春大麦的效果。方法:1)采用不同施肥方式对草坪砂质土进行田间试验;2)沙砂基质温室试验,15N同位素稀释分析;3)在固定式渗析装置中进行渗析实验。此外,色谱法测定植物根区细菌的氮酶活性,农化和统计方法。结果。在田间条件下,在不施肥的“土壤-植物”体系中,接种巴西螺410能显著提高氮酶活性(37 ~ 103%),特别是在使用N60P60K60的土壤-植物体系中。高施肥量(N120P120K120)导致氮素活性的长期抑制。在两种情况下(接种和未接种巴西芽孢杆菌410的植株),这种施肥水平仅在植被期结束时显示出氮酶活性的增加。接种菌株410后,以N60P60K60施肥的田块产量增幅最大(0.7 t/ hm2, 27%);不施肥小区增产最少(0.33吨/公顷,16.5%)。播前接种可使大麦籽粒中蛋白质含量增加0.3 ~ 1.0%,特别是在高施肥水平时,提高了其谷物和饲料的使用价值,但降低了其酿造的使用价值。15N的温室试验表明,由于固氮过程的激活,植株的氮素摄入量增加了77.1%,肥料的氮素消耗增加了29.5%。溶出测定结果表明,用巴西芽孢杆菌410接种春大麦可使氮、钾、钙、锰、水溶有机质的垂直迁移和淋溶量分别减少27 ~ 30%、13 ~ 30%、32 ~ 51%、33 ~ 100%和46 ~ 75%。结论。春大麦cv播前接种。Nosivsky种子与a。巴西螺410由于积极固氮和更好地利用矿质肥料中的氮,使植株的氮消耗在29.5%以内。在我们有限的试验中,大麦产量增加0.7 t/ hm2,使用一个品种的增产量大致相当于施用N60P60K60后的增产量。因此,播前接种巴西麻410可以在实践中大幅度减少矿物肥料的使用。
{"title":"The effect of inoculation with Azospirillum brasilense strain 410 on spring barley cv. nosivsky development and yield","authors":"V. Volkogon, A. Moskalenko, S. Dimova, K. Volkogon, L. Potapienko","doi":"10.15407/agrisp9.03.064","DOIUrl":"https://doi.org/10.15407/agrisp9.03.064","url":null,"abstract":"Aim. To study the efficiency of inoculating spring barley with Azospirillum brasilense 410. Methods. 1) A field ex-\u0000periment on turfpodzolic sandy soil with different mineral fertilization regimes; 2) A greenhouse experiment on sand\u0000as substrate, with isotope dilution analysis using 15N; 3) A lysimetric experiment in a stationary lysimetric installation.\u0000Furthermore, chromatography to determine nitrogenase activity of bacteria in the root zone of plants, agrochemical,\u0000and statistical methods. Results. Under field conditions, the inoculation with A. brasilense 410 promoted a significant\u0000increase (37–103 %) in the nitrogenase activity in the “soil-plant” system without any mineral fertilization and (espe-\u0000cially) where N60P60K60 was used. A high fertilizer level (N120P120K120) lead to a long-term inhibition of the nitrogenase\u0000activity. In both cases (plants with and without inoculation with A. brasilense 410) this fertilization level showed an\u0000increase in the nitrogenase activity only at the end of the vegetation period. The highest increase in yield (0.7 t/ha,\u000027 %) in yield following A. brasilense strain 410 inoculation, occurred in plots with N60P60K60 fertilization; the least\u0000increase in yield (0.33 t/ha, 16.5 %) was observed in plots receiving no fertilizers. The pre-sowing inoculation led\u0000to an increase in the protein content of 0.3–1.0 % in the barley grain, especially when receiving high fertilization\u0000levels, enhancing its value for the use in cereals and feeds, but decreasing its value for its use in brewing. The green-\u0000house experiment with 15N established an increase 77.1 % in the nitrogen intake into the plants due to the activation\u0000of the nitrogen-fixation process and enhanced 29.5 % nitrogen consumption from fertilizers. The lysimetric studies\u0000demonstrated that inoculation of spring barley cv Nosivsky with A. brasilense 410 limited the vertical migration and\u0000leaching of nitrogen by 27–30 %, potassium by 13–30, calcium by 32–51 %, manganese by 33–100 %, and water-sol-\u0000uble organic matter by 46–75 %. Conclusions. The pre-sowing inoculation of spring barley cv. Nosivsky seeds with\u0000A. brasilense 410 intensifies nitrogen consumption by plants within 29.5 % due to active nitrogen-fixation and a better\u0000utilization of N from mineral fertilizers. The barley yield increase with 0.7 t/ha was in our limited experiment, using\u0000one cultivar roughly equivalent to the increase after mineral fertilization with N60P60K60. Thus, pre-sowing inoculation\u0000with A. brasilense 410 may lead to substantial reduction of the use of mineral fertilisers in practice.","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48837307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Mutation types and frequency in Nigella damascena L. in the М2 and M3 generation, using ethyl methanesulfonate, nitrosomethylurea and a new derivative of dimethylsulfate, DG-2 使用甲磺酸乙酯、亚硝基甲基脲和一种新的二甲基硫酸衍生物DG-2的大麦Nigella damascena L.在М2和M3代中的突变类型和频率
IF 0.4 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-04-20 DOI: 10.15407/agrisp9.03.049
Yu. O. Gubanova
Aim. To identify mutations and evaluate the mutation frequency in Nigella damascena L. cultivars (cvs) Bereginyaand Charivnytsya (M2 and M3 generation), following treatment of their seeds with ethyl methanesulfonate (EMS),nitrosomethylurea (NMU) and a new derivative of dimethyl sulfate, DG-2. Methods. Treated Nigella seeds of twocvs with the mutagens for 6 and 16 h and in concentrations of 0.01 and 0.5 % for EMS and NMU and 0.05 and 0.5 %for DG-2. Results. A wide range of mutations (59 types) was obtained, that was divided into six groups: five groupswith changes in the morphological type and one group with changes in the physiological type. Among the detectedmutations, there were both previously known mutations and those obtained in this culture for the first time. The high-est mutation frequency (30 %) affecting synthesis of chlorophyll and structure of stem, shoots and leaves and 20 % forphysiological features, was registered for NMU at 16 h and 0.05 % in cv. Bereginya. However, this NMU concentra-tion appeared to be lethal for cv. Charivnytsya. Conclusions. The new mutagen DG-2 proved to be most effective forinducing mutations in the corolla petal color of nigella, namely 4.0 at a 0.5 % concentration of the mutagen and 16hexposure for cv. Bereginya and 4.0 % at the same concentration and exposure for cv. Charivnytsya. DG-2 caused asubstantial number of mutations in all six mutation groups affecting morphological and physiological traits. The clas-sic mutagen EMS was also effective across the spectrum of mutation groups in our study; however, it caused mutationsat a lower frequency. The maximum mutation frequency under influence of EMC at a concentration of 0.05 % and anexposure of 16 h in cv. Bereginya was 11.0 %, and in cv. Charivnytsya 8.0 %. For all three mutagens used, an increasein the concentration of the active substance and of exposure time led to an increase in the mutation frequency in N.damascena plants. We will select mutants with economically valuable traits, such as tall, lodging-resistant plants andearly maturing ones, for further work on the development of new cultivars of N. damascena for industrial cultivation.
目标用甲磺酸乙酯(EMS)、亚硝甲基脲(NMU)和新的硫酸二甲酯衍生物DG-2处理奈杰尔拉(Nigella damascena L.)品种Bereginya和Charivnytsya(M2和M3代)的种子后,鉴定其突变并评估其突变频率。方法。用诱变剂处理两个cvs的Nigella种子6和16小时,EMS和NMU的浓度分别为0.01%和0.5%,DG-2的浓度分别是0.05%和0.5%。后果获得了广泛的突变(59种类型),分为六组:五组形态类型发生变化,一组生理类型发生变化。在检测到的突变中,既有先前已知的突变,也有首次在该培养物中获得的突变。影响叶绿素合成和茎、芽、叶结构的突变频率最高(30%),影响生理特征的突变频率为20%。然而,这种NMU浓度似乎对Charivnytsya变种是致命的。结论。新的诱变剂DG-2被证明是最有效的诱导nigela花冠花瓣颜色的突变,即在0.5%的诱变物和16hexposure浓度下,Bereginya为4.0,在相同浓度和暴露条件下,Charivnytsya为4.0%。DG-2在影响形态和生理性状的所有六个突变组中都引起了大量突变。在我们的研究中,分类诱变剂EMS在整个突变组中也是有效的;然而,它引起突变的频率较低。在0.05%浓度和暴露16h的EMC影响下,Bereginya和Charivnytsya的最大突变频率分别为11.0%和8.0%。对于所使用的所有三种诱变剂,活性物质浓度和暴露时间的增加导致N.damascena植物的突变频率增加。我们将选择具有经济价值性状的突变体,如高大、抗倒伏和早熟植株,以进一步开发用于工业栽培的大马士革N.damascena新品种。
{"title":"Mutation types and frequency in Nigella damascena L. in the М2 and M3 generation, using ethyl methanesulfonate, nitrosomethylurea and a new derivative of dimethylsulfate, DG-2","authors":"Yu. O. Gubanova","doi":"10.15407/agrisp9.03.049","DOIUrl":"https://doi.org/10.15407/agrisp9.03.049","url":null,"abstract":"Aim. To identify mutations and evaluate the mutation frequency in Nigella damascena L. cultivars (cvs) Bereginya\u0000and Charivnytsya (M2 and M3 generation), following treatment of their seeds with ethyl methanesulfonate (EMS),\u0000nitrosomethylurea (NMU) and a new derivative of dimethyl sulfate, DG-2. Methods. Treated Nigella seeds of two\u0000cvs with the mutagens for 6 and 16 h and in concentrations of 0.01 and 0.5 % for EMS and NMU and 0.05 and 0.5 %\u0000for DG-2. Results. A wide range of mutations (59 types) was obtained, that was divided into six groups: five groups\u0000with changes in the morphological type and one group with changes in the physiological type. Among the detected\u0000mutations, there were both previously known mutations and those obtained in this culture for the first time. The high-\u0000est mutation frequency (30 %) affecting synthesis of chlorophyll and structure of stem, shoots and leaves and 20 % for\u0000physiological features, was registered for NMU at 16 h and 0.05 % in cv. Bereginya. However, this NMU concentra-\u0000tion appeared to be lethal for cv. Charivnytsya. Conclusions. The new mutagen DG-2 proved to be most effective for\u0000inducing mutations in the corolla petal color of nigella, namely 4.0 at a 0.5 % concentration of the mutagen and 16h\u0000exposure for cv. Bereginya and 4.0 % at the same concentration and exposure for cv. Charivnytsya. DG-2 caused a\u0000substantial number of mutations in all six mutation groups affecting morphological and physiological traits. The clas-\u0000sic mutagen EMS was also effective across the spectrum of mutation groups in our study; however, it caused mutations\u0000at a lower frequency. The maximum mutation frequency under influence of EMC at a concentration of 0.05 % and an\u0000exposure of 16 h in cv. Bereginya was 11.0 %, and in cv. Charivnytsya 8.0 %. For all three mutagens used, an increase\u0000in the concentration of the active substance and of exposure time led to an increase in the mutation frequency in N.\u0000damascena plants. We will select mutants with economically valuable traits, such as tall, lodging-resistant plants and\u0000early maturing ones, for further work on the development of new cultivars of N. damascena for industrial cultivation.","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44377644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An acid-base buffering model to describe pH buffering capacity of an acid albic stagnic luvisol under long-term agricultural land use and management 用酸碱缓冲模型描述了长期农用地利用和管理条件下,酸-白-静态陆维醇的pH缓冲能力
IF 0.4 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-04-20 DOI: 10.15407/agrisp9.03.018
Y. Olifir, A. Habryel, T. Partyka, O. Havryshko, G. Konyk, N. Kozak, V. Lykhochvor
Aim. To study acid-base buffering capacity depending on the intensity of different fertilization systems, including lim-ing, with different doses of CaCO3 in a long-term (55 years) stationary experiment on Albic Stagnic Luvisol (light greyforest surface-gleyed soil). This study should serve as a basis for the restoration and protection of fertility and yieldand preservation of ecological restorative functions of this soil type. Methods. Field stationary experiment, usingmonitoring, laboratory-analytical and statistical-mathematical methods. Results. It was found that after 35 years of aseven-field crop rotation the exclusion of the intensive crops of sugar beet, potato and one winter wheat, in combina-tion with low (2.5 time less) mineral fertilization levels, contributed to an increase in resistance to acidification overthe next 20 years from 5.53 to 7.48 points (using a 100-point scale) with a simultaneous increase in soil рНКСl from3.77 to 4.12. Organo-mineral fertilization (N65P68K68 + 10 t manure/ha of crop rotation area) and periodic applicationof CaCO3 by hydrolytic acidity (6.0 t/ha) and an optimal dose of lime (2.5 t/ha CaCO3) increased pH buffering overthese 20 years in a four-field rotation. The general evaluation index of buffering was 21.8–21.9 points, exceedingthe virgin soil by 1.9 to 2 points. In the control variants without the use of fertilizers the general evaluation index ofbuffering was 14.3 ± 0.3, and the coefficient of buffer asymmetry was the highest – 0.646 ± 0.013, which under theseconditions indicated the danger of soil losing its ability for self-regulation and self-healing. Conclusions. The resis-tance of Albic Stagnic Luvisol to acidification increased most in the combined application of N65P68K68 and 10 t/hamanure, together with an optimum calculated dose of lime in a 4-year crop rotation. An optimal dose of CaCO3 (2.5t/ha) and organo-mineral fertilizing system in a 4-year crop rotation improved the soil buffering capacity of the acidshoulder by 2.45 points compared to the mineral fertilization system. To support a determination of acid-bufferingeffects graphic charts representing pH buffering capacity proved to be useful and could be instrumental in diagnosticsand optimization of the acid-base regime for acid forest soils in general.
目标在Albic Stagnic Luvisol(浅灰色森林表面潜育土壤)上进行了一项长期(55年)的固定实验,研究了不同施肥系统(包括石灰)在不同CaCO3剂量下的酸碱缓冲能力。本研究应为恢复和保护该土壤类型的肥力和产量以及保持其生态恢复功能提供依据。方法。现场固定实验,采用监测、实验室分析和统计数学方法。后果研究发现,经过35年的平均轮作,甜菜、马铃薯和一个冬小麦的集约作物被排除在外,再加上低(2.5倍以下)的矿物质施肥水平,在接下来的20年里,土壤对酸化的抵抗力从5.53点增加到7.48点(使用100分制),同时土壤рНКСl从3.77点增加到4.12点。有机矿物肥料(N65P68K68+10吨肥料/公顷轮作区)和水解酸定期施用CaCO3(6.0吨/公顷)和最佳剂量的石灰(2.5吨/公顷CaCO3)在过去20年的四田轮作中增加了pH缓冲。缓冲综合评价指标为21.8~21.9分,比原状土高1.9~2分。在不使用肥料的对照变体中,缓冲的总体评价指数为14.3±0.3,缓冲不对称系数最高——0.646±0.013,这表明在这些条件下土壤有失去自我调节和自我修复能力的危险。结论。在4年轮作中,N65P68K68和10t/滨氨尿的联合施用,以及石灰的最佳计算剂量,使白蜡鹿膏对酸化的抵抗力增加最多。在4年轮作中,CaCO3(2.5t/ha)和有机矿物施肥系统的最佳剂量比矿物施肥系统提高了酸肩的土壤缓冲能力2.45个百分点。为了支持酸性缓冲作用的测定,代表pH缓冲能力的图表被证明是有用的,并可用于酸性森林土壤酸碱状态的诊断和优化。
{"title":"An acid-base buffering model to describe pH buffering capacity of an acid albic stagnic luvisol under long-term agricultural land use and management","authors":"Y. Olifir, A. Habryel, T. Partyka, O. Havryshko, G. Konyk, N. Kozak, V. Lykhochvor","doi":"10.15407/agrisp9.03.018","DOIUrl":"https://doi.org/10.15407/agrisp9.03.018","url":null,"abstract":"Aim. To study acid-base buffering capacity depending on the intensity of different fertilization systems, including lim-\u0000ing, with different doses of CaCO3 in a long-term (55 years) stationary experiment on Albic Stagnic Luvisol (light grey\u0000forest surface-gleyed soil). This study should serve as a basis for the restoration and protection of fertility and yield\u0000and preservation of ecological restorative functions of this soil type. Methods. Field stationary experiment, using\u0000monitoring, laboratory-analytical and statistical-mathematical methods. Results. It was found that after 35 years of a\u0000seven-field crop rotation the exclusion of the intensive crops of sugar beet, potato and one winter wheat, in combina-\u0000tion with low (2.5 time less) mineral fertilization levels, contributed to an increase in resistance to acidification over\u0000the next 20 years from 5.53 to 7.48 points (using a 100-point scale) with a simultaneous increase in soil рНКСl from\u00003.77 to 4.12. Organo-mineral fertilization (N65P68K68 + 10 t manure/ha of crop rotation area) and periodic application\u0000of CaCO3 by hydrolytic acidity (6.0 t/ha) and an optimal dose of lime (2.5 t/ha CaCO3) increased pH buffering over\u0000these 20 years in a four-field rotation. The general evaluation index of buffering was 21.8–21.9 points, exceeding\u0000the virgin soil by 1.9 to 2 points. In the control variants without the use of fertilizers the general evaluation index of\u0000buffering was 14.3 ± 0.3, and the coefficient of buffer asymmetry was the highest – 0.646 ± 0.013, which under these\u0000conditions indicated the danger of soil losing its ability for self-regulation and self-healing. Conclusions. The resis-\u0000tance of Albic Stagnic Luvisol to acidification increased most in the combined application of N65P68K68 and 10 t/ha\u0000manure, together with an optimum calculated dose of lime in a 4-year crop rotation. An optimal dose of CaCO3 (2.5\u0000t/ha) and organo-mineral fertilizing system in a 4-year crop rotation improved the soil buffering capacity of the acid\u0000shoulder by 2.45 points compared to the mineral fertilization system. To support a determination of acid-buffering\u0000effects graphic charts representing pH buffering capacity proved to be useful and could be instrumental in diagnostics\u0000and optimization of the acid-base regime for acid forest soils in general.","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49130266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Responses of grapevines to planting density and training systems in semiarid environments 半干旱环境下葡萄对种植密度和栽培系统的响应
IF 0.4 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-12-21 DOI: 10.15407/agrisp9.02.038
A. Shtirbu, I. Kovaleva, V. Vlasov
Aim. To investigate the physiological and agrobiological responses of grapevines on different systems of trainingsystems in row plantations depending on the planting density and the possibility of adjusting plants for non-irri-gated crop cultivation under cold semiarid climate. Methods. The field two-way experiment of 2 × 4 was appliedto study the effect of two planting density variants (3 × 1.5 m; 3 × 1 m) and four vine training systems (when thehorizontal cordon is located at the height of 0.4 m, 0.8 m, 1.2 m, 1.6 m) on the parameters of the leaf area, leafindex, water loss through transpiration, activity of the photosynthetic apparatus of leaves and the yield of Zahreiwine grape cultivar (Vitis vinifera L.). The experiment data were processed using the analysis of variance. Results.The increase in the density of vine row plantations per area units from 3 × 1.5 m to 3 × 1 m enlarges the total leafarea, the exposed leaf area, water loss through transpiration, and yield weight, but these indices decrease in theevaluations per plant. The responses of grapevine to the training systems are similar and independent from the ex-perimental variants of planting density. At the height of the cordon of 0.4 and 0.8 m, the vertical shoot positioningenhances the potential of the photosynthetic capacity of the plantations, but this potential is realized only duringthe years with lower water deficit for plants. Free-growing shootings on the cordons, located at the height of 1.2m, form the canopy architecture with relatively low water loss through transpiration which has a positive effecton the activity of the photosynthetic apparatus and yield, especially in dry years. The downward shoot positioningon the cordons of 1.6 m decreases the leaf area of the vines and creates the canopy architecture with increasedtranspiration which enhances the effect of the water deficit and has a negative effect on the productivity of plants.Conclusions. The agronomic methods of planting and training systems for grapevines ensure the management ofthe character of spatial shoot location, the formation of certain canopy architecture, and setting the parameters ofthe leaf area; their optimization mitigates the negative effect of water deficit and provides for adapting the plantsfor non-irrigated crops under semiarid climate. The positive effect of compacting plantations on crop yield wasdetermined without irrigation in semiarid environments. The variant of planting density of 3 × 1 m decreases theyield on the vines on average by 12.1–31.0 %, as compared to the variant of 3 × 1.5 m. Yet, more dense plantationsare remarkable for their yield, which is 18.5–61.3 % higher depending on the training system for vines. Under dryconditions, the most efficient system is the training system with the formation of the horizontal cordon at the heightof 1.2 m and free-growing shoots. The system optimizes the leaf area density, and forms the canopy architecturewith rather lo
目标根据种植密度和在寒冷半干旱气候下调整植物进行非灌溉作物种植的可能性,研究葡萄藤对行种植园不同栽培系统的生理和农业生物学反应。方法。采用2×4的田间双向试验,研究了两种种植密度变化(3×1.5m;3×1m)和四种葡萄栽培系统(当水平警戒线位于0.4m、0.8m、1.2m、1.6m高度时)对叶面积、叶指数、蒸腾失水等参数的影响,用方差分析法对扎赫赖温葡萄品种(Vitis vinifera L.)的叶片光合器活性和产量进行了处理。结果:单位面积葡萄园密度从3×1.5m增加到3×1m,增加了总叶面积、露叶面积、蒸腾失水量和产量,但这些指标降低了单株价值。葡萄藤对训练系统的反应是相似的,并且与种植密度的实验变化无关。在警戒线0.4米和0.8米的高度,垂直的枝条位置提高了人工林光合能力的潜力,但这种潜力只有在植物缺水较低的年份才能实现。警戒线上1.2米高的自由生长的枝条形成了蒸腾损失相对较低的冠层结构,对光合器官的活动和产量有积极影响,尤其是在干旱年份。在1.6米的警戒线上向下的枝条位置减少了葡萄藤的叶面积,并创造了具有增加的蒸腾量的树冠结构,这增强了缺水的影响,并对植物的生产力产生了负面影响。结论。葡萄种植和栽培系统的农艺方法确保了对空间枝条位置特征的管理、一定冠层结构的形成以及叶面积参数的设置;它们的优化减轻了缺水的负面影响,并为在半干旱气候下种植非灌溉作物提供了条件。在半干旱环境中,在不灌溉的情况下确定了压实人工林对作物产量的积极影响。与3×1.5m的种植密度相比,3×1m的种植密度变化使葡萄藤的产量平均降低了12.1-31.0%。然而,更密集的种植密度对其产量来说是显著的,根据葡萄藤的训练系统,产量高出18.5-61.3%。在干旱条件下,最有效的系统是在1.2米的高度形成水平警戒线和自由生长的嫩芽的训练系统。该系统优化了叶面积密度,并通过蒸腾作用形成了水分损失较低的冠层结构,这对干旱期间叶片光合作用的活性有积极影响。在自由生长条件下,与垂直枝条定位相比,人工林的产量平均增加了4.3-12.3%,在向下定位的情况下,产量平均增加21.3%。
{"title":"Responses of grapevines to planting density and training systems in semiarid environments","authors":"A. Shtirbu, I. Kovaleva, V. Vlasov","doi":"10.15407/agrisp9.02.038","DOIUrl":"https://doi.org/10.15407/agrisp9.02.038","url":null,"abstract":"Aim. To investigate the physiological and agrobiological responses of grapevines on different systems of training\u0000systems in row plantations depending on the planting density and the possibility of adjusting plants for non-irri-\u0000gated crop cultivation under cold semiarid climate. Methods. The field two-way experiment of 2 × 4 was applied\u0000to study the effect of two planting density variants (3 × 1.5 m; 3 × 1 m) and four vine training systems (when the\u0000horizontal cordon is located at the height of 0.4 m, 0.8 m, 1.2 m, 1.6 m) on the parameters of the leaf area, leaf\u0000index, water loss through transpiration, activity of the photosynthetic apparatus of leaves and the yield of Zahrei\u0000wine grape cultivar (Vitis vinifera L.). The experiment data were processed using the analysis of variance. Results.\u0000The increase in the density of vine row plantations per area units from 3 × 1.5 m to 3 × 1 m enlarges the total leaf\u0000area, the exposed leaf area, water loss through transpiration, and yield weight, but these indices decrease in the\u0000evaluations per plant. The responses of grapevine to the training systems are similar and independent from the ex-\u0000perimental variants of planting density. At the height of the cordon of 0.4 and 0.8 m, the vertical shoot positioning\u0000enhances the potential of the photosynthetic capacity of the plantations, but this potential is realized only during\u0000the years with lower water deficit for plants. Free-growing shootings on the cordons, located at the height of 1.2\u0000m, form the canopy architecture with relatively low water loss through transpiration which has a positive effect\u0000on the activity of the photosynthetic apparatus and yield, especially in dry years. The downward shoot positioning\u0000on the cordons of 1.6 m decreases the leaf area of the vines and creates the canopy architecture with increased\u0000transpiration which enhances the effect of the water deficit and has a negative effect on the productivity of plants.\u0000Conclusions. The agronomic methods of planting and training systems for grapevines ensure the management of\u0000the character of spatial shoot location, the formation of certain canopy architecture, and setting the parameters of\u0000the leaf area; their optimization mitigates the negative effect of water deficit and provides for adapting the plants\u0000for non-irrigated crops under semiarid climate. The positive effect of compacting plantations on crop yield was\u0000determined without irrigation in semiarid environments. The variant of planting density of 3 × 1 m decreases the\u0000yield on the vines on average by 12.1–31.0 %, as compared to the variant of 3 × 1.5 m. Yet, more dense plantations\u0000are remarkable for their yield, which is 18.5–61.3 % higher depending on the training system for vines. Under dry\u0000conditions, the most efficient system is the training system with the formation of the horizontal cordon at the height\u0000of 1.2 m and free-growing shoots. The system optimizes the leaf area density, and forms the canopy architecture\u0000with rather lo","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48402927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effectiveness of using the mixtures of herbicides flumioxazine and fluorochloridone in sunflower crops 氟恶嗪与氟氯酮混用除草剂在向日葵作物上的效果
IF 0.4 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-12-21 DOI: 10.15407/agrisp9.02.023
M. Radchenko, Zh.z. Guralchuk, O. Rodzevych, M. Khandezhina, Y. Morderer
Aim. This work is devoted to the search for potential partners for the integrated application of flumioxazine insunflower crops. This herbicide is an inhibitor of protoporphyrinogen oxidase (PPO) in weed plants. The resistanceto this class of herbicides is not yet common today, compared to other classes. Therefore, the work is aimed at de-veloping flumioxazine-based herbicide compositions that effectively prevent the emergence of herbicide-resistantweed biotypes. Methods. The interaction effects, weed control efficiency, and crop selectivity were studied whenflumioxazine was used in the mixtures with herbicides acetochlor and propisochlor (long-chain fatty acid synthesis(LCFAS) inhibitors), promethrin (electron transport (ET) inhibitor in photosystem 2 (PS 2) of chloroplasts) andfluorochloridone (inhibitor of carotenoid synthesis by blocking the activity of phytoendesaturases (PDS)) both inthe experiments on sunflower crops and in greenhouse experiments using the model objects. Results. The studieshave shown that when flumioxazine is applied with the ET inhibitor, promethrin, the interaction is antagonistic,resulting in poor weed control efficiency and sunflower yield decrease. The tank mixtures of flumioxazine withLCFAS inhibitors acetochlor and propisochlor provide high weed control efficiency but are low in selectivity forsunflowers. The interaction between flumioxazine and fluorochloridone within the recommended application ratesis additive. The tank mixture of flumioxazine and fluorochloridone herbicides at the application rates of 55 and 500g/ha, respectively, is selective for sunflowers. In terms of control efficiency of annual dicotyledons, this mixture ofherbicides exceeded, and in terms of control efficiency of annual cereal weed species was only slightly inferior tothe control integrated herbicide (metolachlor + terbuthylazine). In these application rates, flumioxazine and fluo-rochloridone provided sunflower yield on par with this complex herbicide. Сonclusions. Among the investigatedherbicides, the optimal partner of flumioxazine for complex use in sunflower crops was the herbicide, inhibitorPDS, fluorochloridone. Given that flumioxazine and fluorochloridone differ in their phytotoxicity mechanismsbut share a common spectrum of controlled weed species, the use of a mixture of these herbicides is a factor thatminimizes the likelihood of the emergence of herbicide-resistant weed biotypes.
的目标。本工作致力于寻找氟恶嗪在向日葵作物上综合应用的潜在合作伙伴。该除草剂是杂草植物原卟啉原氧化酶(PPO)的抑制剂。与其他种类的除草剂相比,对这类除草剂的抗性目前还不常见。因此,本工作旨在开发基于氟恶嗪的除草剂组合物,以有效防止除草剂抗性杂草生物型的出现。方法。研究了氟恶嗪与除草剂乙草胺、异丙氯(长链脂肪酸合成抑制剂)混用时的互作效应、除草效果和作物选择性。除虫菊酯(叶绿体光系统2 (PS 2)中的电子传递(ET)抑制剂)和氟氯酮(通过阻断植物endesaturase (PDS)活性合成类胡萝卜素的抑制剂)在向日葵作物实验和使用模型对象的温室实验中均得到证实。结果。研究表明,氟恶嗪与ET抑制剂丙氰菊酯施用时,相互作用为拮抗作用,导致防杂草效果差,向日葵产量下降。氟恶嗪与lcfas抑制剂乙草胺和异丙氯的罐体混合对向日葵的杂草控制效率高,但对向日葵的选择性较低。氟恶嗪与氟氯酮在推荐用量范围内的相互作用。氟恶嗪和氟氯酮两种除草剂的罐内混合物,施用量分别为55 g/ha和500g/ha,对向日葵具有选择性。在对一年生双子叶植物的防治效率方面,该混合除草剂超过了对一年生谷物杂草的防治效率,而在对一年生谷物杂草的防治效率方面,该混合除草剂仅略低于对照组合除草剂(甲草胺+特丁基嗪)。在这些施用量下,氟恶嗪和氟氯酮提供的向日葵产量与这种复合除草剂相当。Сonclusions。在所研究的除草剂中,氟恶嗪在向日葵作物中复合使用的最佳搭档是除草剂、抑制剂或pds、氟氯酮。鉴于氟恶嗪和氟氯酮的植物毒性机制不同,但受控制的杂草种类有共同的范围,使用这些除草剂的混合物是一个最大限度地减少出现抗除草剂杂草生物型的可能性的因素。
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引用次数: 0
Investigation of species composition of the fungi of the Fusarium genus and the resistance of the Chornobyl radio-mutants to fusarium head blight for the purposes of winter wheat 冬小麦镰刀菌属真菌的种类组成及钩藤放射突变体对枯萎病的抗性研究
IF 0.4 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-12-21 DOI: 10.15407/agrisp9.02.051
Y. Dolhalova, L. Burdeynyuk-Tarasevych, O. Zozulya, M. Lozinskyi, O. Hrytsev, M. Buzynnyi
Aim. To investigate the field resistance of collection spelt-like samples of the Chornobyl radio-mutants (RM) ofwinter wheat to Fusarium head blight and to determine the species composition of the fungi of the Fusarium genusin the Forest-Steppe of Ukraine. Methods. The resistance (in scores) of ten RM-samples of winter wheat to Fusar-ium head blight was determined in field conditions by visual inspection of plants using standard methods (Trybelet al, 2010). To determine the infection rate of the seeds, the biological method of seed germination in the rolls offiltration paper was used. DNA was extracted using the reaction kit AGROSORB NK (Agrogen Novo, Ukraine),according to the manufacturer’s instructions. The molecular identification of the species composition of the fungiof the Fusarium genus was conducted with the commercial test systems “Fuzarioz zlakiv” (AgroDiagnostica,RU) according to the manufacturer’s instructions, using the real-time PCR. Results. The results of evaluating thefield resistance of ten spelt-like RM-samples of winter wheat to Fusarium head blight are presented. The speciescomposition of the Fusarium fungi as of 2016–2018 in the Forest-Steppe of Ukraine, namely in Kyiv, Cherkasy,Sumy, Vinnytsia, Chernivtsi, Poltava, Kharkiv, Ternopil, and Khmelnytsky regions is presented. To study the spe-cies composition of the Fusarium fungi, 639 seed samples of winter wheat were taken from the farms located inthe territory of nine regions in the Forest-Steppe zone. The results of molecular identification of the infection ratein the seed material demonstrate the presence of seven out of eight fungi species under investigation: F. avena-ceum, F. culmorum, F. graminearum, F. langsethiae, F. poae, F. sporotrichioides, F. tricinctum during the yearsof studies. Yet, F. culmorum, F. langsethiae were not identified in 2017, and F. cerealis was not found during thestudy years, in 2016–2018. It should be noted that the most frequent incidence was found for F. graminearum, F.avenaceum and F. tricinctum, which was within the range of 25.8–44.0 %, and according to (Zhdanova, 2002) itrefers to the group of frequently dispersed species. The results of the PCR diagnostics of the species compositionof Fusarium fungi in Kyiv region, where field studies were conducted to evaluate the resistance of collection spelt-like Chornobyl radio-mutants in 2016–2018 (Bila Tserkva Breeding Research Station (BTBRS) of the Instituteof Bioenergy Crops and Sugar Beet of the NAAS (Mala Vilshanka village, Bila Tserkva district, Kyiv region)demonstrated the spreading of six species of Fusarium fungi: F. graminearum, F. avenaceum, F. sporotrichioides,F. poae, F. tricinctum, F. culmorum, among which high aggressiveness to the wheat spikelets was observed inF. avenaceum, F. culmorum, F. graminearum; medium aggressiveness – in F. poae, and low aggressiveness – inF. sporotrichioides, F. tricinctum. On the background of the obtained species diversity of th
的目标。目的研究乌克兰森林草原地区冬小麦放射性突变体(RM)对赤霉病的田间抗性,并确定赤霉病属真菌的种类组成。方法。在田间条件下,采用标准方法对植株进行目测,测定了10份冬小麦rmm样品对镰刀菌头疫病的抗性(以分值计)(Trybelet al ., 2010)。为测定种子侵染率,采用生物法测定种子在卷式滤纸中的萌发。根据制造商的说明,使用AGROSORB NK反应试剂盒(Agrogen Novo,乌克兰)提取DNA。根据制造商的说明,使用商业测试系统“Fuzarioz zlakiv”(agrodiagnostics,RU),使用实时PCR对镰刀菌属真菌的物种组成进行分子鉴定。结果。本文报道了10个冬小麦品种的田间抗枯萎病性评价结果。本文介绍了2016-2018年乌克兰森林草原镰刀菌的种类组成,即基辅、切尔卡西、苏梅、文尼察、切尔诺夫西、波尔塔瓦、哈尔科夫、捷尔诺波尔和赫梅利尼茨基地区。为了研究镰孢菌的种类组成,从森林草原区9个地区的农场采集了639份冬小麦种子样品。种子材料中侵染率的分子鉴定结果表明,在研究的8种真菌中,有7种真菌存在:avena-ceum、F. culmorum、F. graminearum、F. langsethiae、F. poae、F. sporotrichiides、F. tricinctum。然而,F. culmorum和F. langsethiae在2017年没有被发现,F. cerealis在2016-2018年的研究期间没有被发现。值得注意的是,f.g raminearum、f.g avenaceum和f.g tricinctum的发病率最高,在25.8% - 44.0%之间,根据(Zhdanova, 2002),它指的是频繁分散的物种群。2016-2018年,乌克兰国家农业科学院生物能源作物和甜菜研究所(基辅地区Bila Tserkva区Mala Vilshanka村)的Bila Tserkva育种研究站(BTBRS)对基辅地区镰刀菌进行了抗性田间研究,对该地区镰刀菌的种类组成进行了PCR诊断,结果显示有6种镰刀菌在传播:禾本科F. graminearum, avenaceum, sporotrichiides,F.;其中,对小麦小穗具有较强侵袭性的有:avenaceum、F. culmorum、F. graminearum;中等侵略性-在F. poae,和低侵略性-在F. sporotrichiides, F. tricinctum。在获得病害病原物种多样性的基础上,我们对10个冬小麦品种进行了田间抗病性评价,这些品种的抗病性在7.4 ~ 8.4分之间波动。在最有利于病害发展的气候条件下,2016年(从3月1日春季植被恢复到7月9日籽粒完全成熟期间,降水量达404.2 mm,超过常年指标175%)调查样品:RM-1、RM-3、RM-4、RM-5、RM-6、RM-9、RM-10的抗性超过标准品种Pisnia - 7.0分。结论。与镰刀菌的种类组成和地理分布有关的实地和实验室研究为假设在乌克兰森林草原的九个地区存在广泛的镰刀菌种复群提供了依据:F. avenaceum, F. culmorum, F. graminearum, F. langsethiae, F. poae, F. sporotrichioides, F. tricinctum。利用镰刀菌群生物学特性与其所处环境的气候条件之间的密切关系,研究了2016-2018年10份收集的切尔诺贝利辐射突变体的抗性,并分离出5份冬小麦(RM-9、RM-5、RM-6、RM-10、RM-4)的抗性,这些样品对镰刀菌头疫病的抗性平均高于标准品种Lisova Pisnia。值得注意的是,在2016年有利于病原菌生长的天气条件下,RM-3和RM-4的抗性明显高于标准。我们将研究年份(2016-2018年)平均抗性指数较高的5个拼版型冬小麦样品(RM-9、RM-5、RM-6、RM-10、RM-4)引入育种计划,作为获得抗赤霉病品种的初始材料。
{"title":"Investigation of species composition of the fungi of the Fusarium genus and the resistance of the Chornobyl radio-mutants to fusarium head blight for the purposes of winter wheat","authors":"Y. Dolhalova, L. Burdeynyuk-Tarasevych, O. Zozulya, M. Lozinskyi, O. Hrytsev, M. Buzynnyi","doi":"10.15407/agrisp9.02.051","DOIUrl":"https://doi.org/10.15407/agrisp9.02.051","url":null,"abstract":"Aim. To investigate the field resistance of collection spelt-like samples of the Chornobyl radio-mutants (RM) of\u0000winter wheat to Fusarium head blight and to determine the species composition of the fungi of the Fusarium genus\u0000in the Forest-Steppe of Ukraine. Methods. The resistance (in scores) of ten RM-samples of winter wheat to Fusar-\u0000ium head blight was determined in field conditions by visual inspection of plants using standard methods (Trybel\u0000et al, 2010). To determine the infection rate of the seeds, the biological method of seed germination in the rolls of\u0000filtration paper was used. DNA was extracted using the reaction kit AGROSORB NK (Agrogen Novo, Ukraine),\u0000according to the manufacturer’s instructions. The molecular identification of the species composition of the fungi\u0000of the Fusarium genus was conducted with the commercial test systems “Fuzarioz zlakiv” (AgroDiagnostica,\u0000RU) according to the manufacturer’s instructions, using the real-time PCR. Results. The results of evaluating the\u0000field resistance of ten spelt-like RM-samples of winter wheat to Fusarium head blight are presented. The species\u0000composition of the Fusarium fungi as of 2016–2018 in the Forest-Steppe of Ukraine, namely in Kyiv, Cherkasy,\u0000Sumy, Vinnytsia, Chernivtsi, Poltava, Kharkiv, Ternopil, and Khmelnytsky regions is presented. To study the spe-\u0000cies composition of the Fusarium fungi, 639 seed samples of winter wheat were taken from the farms located in\u0000the territory of nine regions in the Forest-Steppe zone. The results of molecular identification of the infection rate\u0000in the seed material demonstrate the presence of seven out of eight fungi species under investigation: F. avena-\u0000ceum, F. culmorum, F. graminearum, F. langsethiae, F. poae, F. sporotrichioides, F. tricinctum during the years\u0000of studies. Yet, F. culmorum, F. langsethiae were not identified in 2017, and F. cerealis was not found during the\u0000study years, in 2016–2018. It should be noted that the most frequent incidence was found for F. graminearum, F.\u0000avenaceum and F. tricinctum, which was within the range of 25.8–44.0 %, and according to (Zhdanova, 2002) it\u0000refers to the group of frequently dispersed species. The results of the PCR diagnostics of the species composition\u0000of Fusarium fungi in Kyiv region, where field studies were conducted to evaluate the resistance of collection spelt-\u0000like Chornobyl radio-mutants in 2016–2018 (Bila Tserkva Breeding Research Station (BTBRS) of the Institute\u0000of Bioenergy Crops and Sugar Beet of the NAAS (Mala Vilshanka village, Bila Tserkva district, Kyiv region)\u0000demonstrated the spreading of six species of Fusarium fungi: F. graminearum, F. avenaceum, F. sporotrichioides,\u0000F. poae, F. tricinctum, F. culmorum, among which high aggressiveness to the wheat spikelets was observed in\u0000F. avenaceum, F. culmorum, F. graminearum; medium aggressiveness – in F. poae, and low aggressiveness – in\u0000F. sporotrichioides, F. tricinctum. On the background of the obtained species diversity of th","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42578275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
State of water-stable soil structure in the Central Forest-Steppe under agrogenic and postagrogenic maintenance 耕后维持下中央森林草原水稳性土壤结构状况
IF 0.4 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-12-21 DOI: 10.15407/agrisp9.02.003
S. Y. B. S. Yu. Bulyhin, O. Demydenko, M. Tkachenko, S. Vitvitsky, Ye. Zadubynna, M. Lisovyy
Aim. To determine the regularities in the formation of water-stable structure and to obtain the objective integral informationabout the process of break-up and consolidation of the water-stable structure under agrogenic and postagrogenic load on themain soil types of the Central Forest-Steppe of Ukraine. Methods. The field method – the soils of chernozem type wereinvestigated (seven chernozem-like soil types of different granulometric composition and humus content) in the central partof the Forest-Steppe of Ukraine, the laboratory analytical method (wet sieving of soil structure), the mathematical-statisticalmethod (non-parametric statistics, factor, cluster, and fractal analyses). Results. The analysis of the water stability of the structureof chernozem-like soils in the Forest-Steppe demonstrates the perspectives of using modern statistical methods: fractal, factor,cluster methods, and the method of non-parametric statistics, which demonstrates their sensitivity to insignificant changes in thedistribution of water-stable aggregates within the agronomically valuable interval. The soil types of postagrogenic maintenanceform the distribution of water-stable aggregates, making up “prevailing” sizes of aggregates in the interval of sizes of 5–3and 2–1 mm, which ensures the persistent state of the re-distribution where the index of Hurst has the values of H > 0.75.The agrogenic impact on soils changes the re-distribution of water-stable aggregates, destroying their natural distribution andensuring the antipersistent state of the distribution with low stability (D > 1.43 and Н < 0.58). Under postagrogenic maintenanceof soil types, there is a stable mutual connection between water-stable aggregates, structural units, and ESP (R = +0.78), andin the total of correlation relations, the direct and inverse correlation relations of R> ± 0.55 level are >30 % with the 1.5 to 1ratio in favor of inverse proportion relations which ensures a high level of self-regulation for the hierarchical organization ofthe structural and water stable state. Conclusions. The determining index, characterizing the stability level of the water-stablestructure, was found to be the content of water-stable aggregates of 3–1 mm and > 0.25 mm and the weighted average diameterof water-stable aggregates within the agronomically valuable interval of sizes that demonstrated a strong correlation (R = ±0.76–0.96 ± 0.02) with fractal dimensionality (D > 1.4) and the index of Hurst (Н) which allowed for forming a gradation scalefor the evaluation of the water-stable structure and the degree of agrogenesis manifestation of soils in the Forest-Steppe ofUkraine. In terms of the rate of agrogenesis manifestation via the formation of the degree of the water-stable structure, the soilsof chernozem type were divided as follows: gray forest low-humus heavy-loamy light-clay soil on carbonate loess-like clay <typical low-humus heavy-loamy light-clay chernozem on loess-like clay <
目标确定乌克兰中央森林草原主要土壤类型在农业和农业后负荷下水稳定结构形成的规律,并获得水稳定结构破碎和固结过程的客观完整信息。方法。实地方法——对乌克兰森林草原中部的黑钙土类型土壤(七种不同粒度组成和腐殖质含量的类似黑钙土的土壤类型)、实验室分析方法(土壤结构的湿筛)、数理统计方法(非参数统计、因子、聚类和分形分析)进行了调查。后果对森林草原类黑钙土结构的水稳定性分析表明了使用现代统计方法的观点:分形、因子、聚类方法和非参数统计方法,这表明了它们对农业经济学有价值区间内水稳定团聚体分布的微小变化的敏感性。农业成因后维持的土壤类型形成了水稳性团聚体的分布,构成了粒径为5–3和2–1 mm的团聚体的“主要”粒径,这确保了Hurst指数为H>0.75时再分布的持久状态,破坏了它们的自然分布,并保证了低稳定性分布的反持久状态(D>1.43和Н<0.58)。在土壤类型的后农业维持下,水稳性团聚体、结构单元和ESP之间存在稳定的相互联系(R=+0.78),在相关关系的总和中,R>±0.55水平的正相关关系和反相关关系均大于30%,1.5比1的比例有利于反比例关系,这确保了结构和水稳定状态的分级组织具有高度的自调节性。结论。表征水稳定结构的稳定性水平的确定指数,发现3–1 mm和>0.25 mm的水稳性骨料的含量,以及在具有农业价值的尺寸区间内的水稳型骨料的加权平均直径,与分形维数(D>1.4)和赫斯特指数(Н)具有很强的相关性(R=±0.76–0.96±0.02),从而形成用于评估乌克兰森林草原土壤的水分稳定结构和农业发生程度。就通过形成水稳定结构的程度的农业发生表现的速率而言,将黑钙土类型划分为:灰林低腐殖质重壤质轻粘土-碳酸盐黄土样粘土<典型低腐殖质重壤土-轻粘土-黄土样粘土黑钙土<草甸黑钙土-低腐殖质碳酸盐黄土样重壤质土<典型中腐殖质重粘土-黄土黑钙土。
{"title":"State of water-stable soil structure in the Central Forest-Steppe under agrogenic and postagrogenic maintenance","authors":"S. Y. B. S. Yu. Bulyhin, O. Demydenko, M. Tkachenko, S. Vitvitsky, Ye. Zadubynna, M. Lisovyy","doi":"10.15407/agrisp9.02.003","DOIUrl":"https://doi.org/10.15407/agrisp9.02.003","url":null,"abstract":"Aim. To determine the regularities in the formation of water-stable structure and to obtain the objective integral information\u0000about the process of break-up and consolidation of the water-stable structure under agrogenic and postagrogenic load on the\u0000main soil types of the Central Forest-Steppe of Ukraine. Methods. The field method – the soils of chernozem type were\u0000investigated (seven chernozem-like soil types of different granulometric composition and humus content) in the central part\u0000of the Forest-Steppe of Ukraine, the laboratory analytical method (wet sieving of soil structure), the mathematical-statistical\u0000method (non-parametric statistics, factor, cluster, and fractal analyses). Results. The analysis of the water stability of the structure\u0000of chernozem-like soils in the Forest-Steppe demonstrates the perspectives of using modern statistical methods: fractal, factor,\u0000cluster methods, and the method of non-parametric statistics, which demonstrates their sensitivity to insignificant changes in the\u0000distribution of water-stable aggregates within the agronomically valuable interval. The soil types of postagrogenic maintenance\u0000form the distribution of water-stable aggregates, making up “prevailing” sizes of aggregates in the interval of sizes of 5–3\u0000and 2–1 mm, which ensures the persistent state of the re-distribution where the index of Hurst has the values of H &gt; 0.75.\u0000The agrogenic impact on soils changes the re-distribution of water-stable aggregates, destroying their natural distribution and\u0000ensuring the antipersistent state of the distribution with low stability (D &gt; 1.43 and Н &lt; 0.58). Under postagrogenic maintenance\u0000of soil types, there is a stable mutual connection between water-stable aggregates, structural units, and ESP (R = +0.78), and\u0000in the total of correlation relations, the direct and inverse correlation relations of R&gt; ± 0.55 level are &gt;30 % with the 1.5 to 1\u0000ratio in favor of inverse proportion relations which ensures a high level of self-regulation for the hierarchical organization of\u0000the structural and water stable state. Conclusions. The determining index, characterizing the stability level of the water-stable\u0000structure, was found to be the content of water-stable aggregates of 3–1 mm and &gt; 0.25 mm and the weighted average diameter\u0000of water-stable aggregates within the agronomically valuable interval of sizes that demonstrated a strong correlation (R = ±\u00000.76–0.96 ± 0.02) with fractal dimensionality (D &gt; 1.4) and the index of Hurst (Н) which allowed for forming a gradation scale\u0000for the evaluation of the water-stable structure and the degree of agrogenesis manifestation of soils in the Forest-Steppe of\u0000Ukraine. In terms of the rate of agrogenesis manifestation via the formation of the degree of the water-stable structure, the soils\u0000of chernozem type were divided as follows: gray forest low-humus heavy-loamy light-clay soil on carbonate loess-like clay &lt;\u0000typical low-humus heavy-loamy light-clay chernozem on loess-like clay &lt; ","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44160114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dynamics of climate changes and its effect on the performance of cereals according to satellite data 基于卫星数据的气候变化动态及其对谷物生产性能的影响
IF 0.4 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-12-21 DOI: 10.15407/agrisp9.02.064
O. Tarariko, T. Ilienko, T. Kuchma, V. Velychko
Aim. To study the effect of climate changes on the performance of cereals. Methods. Remote, statistical, andanalytic methods. Climate changes were determined by the sum of radiation temperatures of the earth’s surface,calculated using the data of the infrared range (10.3–11.3; 11.4–12.4 μm) of the high precision radiometer AVHRRof the meteorological satellites of the Earth, NOAA. The effect of climate changes on the state of vegetation,including the phenological parameters, such as the beginning, the end, and the length of the vegetation peri-od, was determined by the Normalized Difference Vegetation Index (NDVI) obtained using the data of infrared(0.72–1.1 μm) and red (0.58–0.68 μm) ranges of the abovementioned radiometer. The satellite data are openlyaccessed on the website of STAR NESDIS NOAA – Satellite Applications and Research of NOAA’s National En-vironmental Satellite Data Information Services of the National Ocean and Atmosphere Research Department ofthe USA – http://www.star.nesdis.noaa.gov/smcd/emb/vci/VH/. The dynamics of СО2 concentration in the air wasdetermined using the satellite data of AIRS/Aqua (https://disc.gsfc.nasa.gov/datasets/AIRS3C2M_005/summary),the precipitation dynamics – using the data of ERA5 ECMWF/Copernicus Climate Change Service (https://devel-opers.google.com/earth-engine/datasets/catalog/ECMWF_ERA5_MONTHLY#description). The association be-tween the performance of cereals and satellite data was found by the method of correlation and regression analysis.Results. Using the satellite data, it was found that in 1982–2021, the sum of radiation temperatures of the earth’ssurface reliably increased in the vegetation period (April–September), and the amount of precipitation tended todecrease in the zones of Polissia, Forest-Steppe, and Steppe. The highest tempo of warming was observed in theterritory of the Steppe. In general, the warming had a positive effect on the state of vegetation, according to NDVI,and on the performance of cereals. However, in the recent decade (2010–2020), the positive effect of the warmingon the vegetation state decreased, and according to the NDVI trend, there has been a tendency towards the negativeeffect of the further increase in the temperature, which is especially remarkable for the Steppe and Forest-Steppe.On the contrary, the warming in the Polissia zone created the conditions for the increase in the performance of agri-cultural cereals. Conclusions. From 1982 till 2021, there was a strong tendency towards the increase in the sum ofradiation temperatures of the earth’s surface in the vegetation period and the decrease in the precipitation amountin Polissia, Forest-Steppe, and Steppe. According to the NDVI data, due to the warming, the length of the vegeta-tion period increased by 14–21 days on average, which had a positive effect on the vegetation. Since 2010, theacceleration in the warming tempo has been observed. The average annual increase in the sum of the
目标研究气候变化对谷物性能的影响。方法。远程、统计和分析方法。气候变化是由地球表面辐射温度的总和确定的,使用地球气象卫星NOAA的高精度辐射计AVHRRo的红外范围(10.3–11.3;11.4–12.4μm)数据计算得出。气候变化对植被状态的影响,包括植被周期的开始、结束和长度等酚学参数,是通过利用上述辐射计的红外(0.72–1.1μm)和红色(0.58–0.68μm)范围的数据获得的归一化差异植被指数(NDVI)来确定的。卫星数据可在STAR NESDIS NOAA的网站上公开访问——美国国家海洋和大气研究部NOAA国家环境卫星数据信息服务的卫星应用和研究——http://www.star.nesdis.noaa.gov/smcd/emb/vci/VH/.利用AIRS/Aqua卫星数据测定了空气中СО2浓度的动态变化(https://disc.gsfc.nasa.gov/datasets/AIRS3C2M_005/summary),降水动力学-使用ERA5 ECMWF/哥白尼气候变化服务的数据(https://devel-opers.google.com/earth-engine/datasets/catalog/ECMWF_ERA5_MONTHLY#description)。采用相关分析和回归分析的方法,发现了谷物性能与卫星数据之间的相关性。后果利用卫星数据发现,在1982年至2021年,地表辐射温度的总和在植被期(4月至9月)可靠地增加,而Polisia、森林草原和草原地区的降水量趋于减少。在大草原的热带地区观测到了最高的升温速度。NDVI表示,总体而言,气候变暖对植被状况和谷物性能都有积极影响。然而,在最近十年(2010-2020年),变暖对植被状态的积极影响有所减少,根据NDVI趋势,温度进一步升高有负面影响的趋势,这在草原和森林草原尤为显著。相反,波利西亚地区的变暖为农业文化谷物的性能提高创造了条件。结论。从1982年到2021年,植被期地表辐射温度总和呈上升趋势,波利西亚、森林草原和草原的降水量呈下降趋势。根据NDVI数据,由于气候变暖,植被期平均增加了14-21天,这对植被产生了积极影响。自2010年以来,气候变暖的速度一直在加快。植被期地表辐射温度总和的年均增长率为:Polisia为15-16°С,森林草原为14-18°С,草原为18-20°С。根据这一趋势,如果在不久的将来保持目前1982-2021年气温上升的规律,植被期的温度将进一步升高,波利西亚地表的平均温度总和将达到4000°С,这在1982-2021的森林草原中是显著的,在森林草原中为4440–4600°С,以及高达5800–5900°С的草原。1982–2000年,植被期的变暖对NDVI指数的植被状态和三个自然气候区的谷物表现都有很强的积极影响规律。然而,2000年后,由于夏季高温对作物的负面影响,干旱对植被的影响显著降低,尤其是草原,与前一时期相比,1992-2001年和2012-2011-2021年NDVI的平均增长率分别为9.41%和1.78%。
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引用次数: 1
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Agricultural Science and Practice
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