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Histological and whole-genome sequencing analysis of coronally advanced flap with connective tissue graft for the treatment of gingival recession—A case study 冠状进展皮瓣联合结缔组织移植治疗牙龈退缩的组织学和全基因组测序分析- 1例研究。
IF 0.9 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2023-11-21 DOI: 10.1002/cap.10273
Saravanan Sampoornam Pape Reddy, Balaji Manohar
<div> <section> <h3> Background</h3> <p>Oral tissue morphogenesis is innately determined and genetically controlled by underlying connective tissue. The connective tissue graft (CTG) harvested from palatal donor sites originally retains “regional specificity” and is considered to be responsible for epithelial keratinization.</p> </section> <section> <h3> Methods</h3> <p>This case study presents histological and genomic outcomes of coronally advanced flap (CAF) with CTG for the management of marginal tissue recession (MTR). The tissue samples harvested at 12-month postoperative period, during the debulking procedure of the treated site were subjected to Masson's trichrome staining (MTS) and whole-genome sequencing (WGS).</p> </section> <section> <h3> Results</h3> <p>Clinically, the mean root coverage as compared to baseline at 6 months (90%), 12 months (95%), and 24 months (95%) postoperatively was achieved. Overbulking of treated site was evident even after 12 months. Nevertheless, clinically discernible alterations in epithelial keratinization were not present. Although MTS revealed areas of non-keratinization and para-keratinization, WGS revealed a significant expression of keratinization genes, neural crest, and positional marker genes.</p> </section> <section> <h3> Conclusions</h3> <p>CAF with CTG is still considered the “gold standard” in treatment of MTR. The keratinization of overlying epithelium at sites hitherto where non-keratinized mucosa is advanced over CTG may not be complete even after 12 months. It is evident from the analysis of the reported case that clinically such sites may appear non-keratinized despite the expression of genes for keratinization. The complex interplay between genomic and phenotypic expressions serves as a foundation of biological concepts. Nevertheless, it is imperative to acknowledge that the phenotype of an organism is not solely determined by genetic expression and is often influenced by a complex interaction between genetic background and environmental factors.</p> </section> <section> <h3> Key Summary</h3> <div>The connective tissue serves as a medium for transmitting the genetic code and exerting an influence on the properties of the overlying epithelial tissue. <ul> <li>Concrete evidence implies connective tissue plays a significant role in influencing the keratinization process of the overlying epithelium.</li> <li>Epithelial keratinization following a coronally advanced flap with connective tissue graft is also gover
背景:口腔组织的形态发生是先天决定的,并受潜在结缔组织的遗传控制。从腭供体部位采集的结缔组织移植物(CTG)最初保留了“区域特异性”,被认为是上皮角化的原因。方法:本病例研究介绍冠状晚期皮瓣(CAF)与CTG治疗边缘组织衰退(MTR)的组织学和基因组学结果。术后12个月,在处理部位的减体积过程中收集组织样本,进行马松三色染色(MTS)和全基因组测序(WGS)。结果:在临床上,与基线相比,术后6个月(90%)、12个月(95%)和24个月(95%)的平均根覆盖率达到了。即使在12个月后,治疗部位仍明显膨胀。然而,临床上可识别的上皮角化改变不存在。虽然MTS显示了非角化和角化过度的区域,但WGS显示了角化基因、神经嵴和位置标记基因的显著表达。结论:CAF联合CTG仍被认为是治疗MTR的“金标准”。在未角化的粘膜在CTG上进展的部位,即使在12个月后,上覆上皮的角化也可能不完全。从报告的病例分析中可以明显看出,尽管角化基因表达,但临床上这些部位可能出现非角化。基因组和表型表达之间复杂的相互作用是生物学概念的基础。然而,必须认识到,生物体的表型并不仅仅由遗传表达决定,而且经常受到遗传背景和环境因素之间复杂的相互作用的影响。结缔组织作为传递遗传密码的媒介,对上覆上皮组织的特性产生影响。具体证据表明结缔组织在影响上覆上皮的角化过程中起重要作用。结缔组织移植物冠状晚期皮瓣后上皮角化也受环境因素和移植物内固有电位的影响。基因表达谱的差异可能因个体、牙齿和不同部位而异。
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引用次数: 0
Translational approach to tooth autotransplantation: A 27-year case study 自体牙移植的翻译方法:一个27年的案例研究。
IF 0.9 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2023-11-20 DOI: 10.1002/cap.10272
GiovanPaolo Pini Prato, Debora Franceschi, Riccardo Pace, Riccardo Di Gianfilippo
<div> <section> <h3> Background</h3> <p>The aim of this case report was to present a translational approach to tooth autotransplantation using jiggling forces to enlarge the periodontal ligament (PDL) space before autotransplantation, with the goal of improving treatment success and long-term survival.</p> </section> <section> <h3> Methods</h3> <p>A 23-year-old patient, undergoing orthodontic therapy and with an unrestorable maxillary first molar, was proposed to have a healthy and fully-erupted maxillary third molar transplanted in the socket of the first molar. Jiggling forces were applied to the third molar to enlarge the PDL space and facilitate the preservation of PDL fibers on the root surfaces during the extraction.</p> </section> <section> <h3> Results</h3> <p>Jiggling forces induced hypermobility and widened PDL space of the third molar. The autotransplantation was successful and the patient was followed regularly over a 27-year period. At the 27-year visit, the patient showed optimal chewing function, oral plaque control, and absence of gingivitis. The transplanted molar exhibited periodontal health and absence of mobility. Probing depth of 5 mm and radiographic external root resorption was noted on a localized area of the transplanted tooth which had experienced traumatic and unintentional removal of PDL fibers during the extraction.</p> </section> <section> <h3> Conclusions</h3> <p>A translational approach was proposed by integrating knowledge from the fields of orthodontics, trauma from occlusion, and replantation. It validated the crucial importance of maintaining healthy PDL fibers on the root surface and demonstrated clinically the successful autotransplantation of a fully formed third molar into the socket of a first molar with a retention of 27 years.</p> </section> <section> <h3> Key points</h3> <div><b>Why is this case new information?</b> <ul> <li>This case provided evidence of successful autotransplantation of a molar with complete root formation. It reported the longest-term follow-up (27 years) present in the literature. Most importantly, it used a translational medicine approach to apply concepts from the fields of orthodontics and traumatic occlusion to improve the success of the autotransplantation procedure.</li> </ul> </div> <div><b>What are the keys to the successful management of this case?</b> <ul> <li>Jiggling forces induced tooth
背景:本病例报告的目的是介绍一种在自体移植前利用振动力扩大牙周韧带(PDL)间隙的平移方法,目的是提高治疗成功率和长期生存率。方法:对一名23岁的上颌第一磨牙无法修复的患者,建议在第一磨牙槽内移植一颗健康且完全发育的上颌第三磨牙。在拔牙过程中,对第三磨牙施加振动力,扩大PDL空间,促进PDL纤维在根表面的保存。结果:振动力导致第三磨牙活动过度,并使其PDL间隙变宽。自体移植是成功的,患者在27年期间定期随访。在27年的随访中,患者表现出最佳的咀嚼功能,口腔菌斑控制,没有牙龈炎。移植磨牙表现出牙周健康和不活动。在移植牙的局部区域,在拔牙过程中经历了外伤性和非故意的PDL纤维去除,探测深度为5mm, x线摄影显示外根吸收。结论:通过整合正畸学、咬合创伤和再植领域的知识,提出了一种翻译方法。它验证了在根表面保持健康的PDL纤维的重要性,并证明了临床成功地将完全形成的第三磨牙移植到第一磨牙的窝中,并保持了27年。重点:为什么这个案例是新信息?本病例提供了磨牙自体移植成功且牙根形成完整的证据。报告了目前文献中最长的随访时间(27年)。最重要的是,它采用了转化医学的方法,应用正畸学和创伤性咬合领域的概念来提高自体移植手术的成功率。成功处理此案的关键是什么?振动力导致牙齿过度活动,增加了计划用于自体移植的牙齿的PDL空间。反过来,他们促进了PDL纤维在移植牙上的非创伤性拔出和保存,提高了牙周纤维再附着的成功率。在这种情况下,成功的主要限制是什么?外伤性拔牙会导致原计划用于自体移植的PDL纤维的意外移除,或者在牙根刨平术中有意移除PDL纤维,预计会降低自体移植手术的成功率。这是由于缺乏再附着所需的有活力的PDL细胞。
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引用次数: 0
Perigraftitis and implant therapy: A case report 周炎与植体治疗1例。
IF 0.9 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2023-11-20 DOI: 10.1002/cap.10271
Jonathan H. Do, Charles M. Cobb

Background

A previous case study reported periimplantitis and concomitant perigraftitis of a second implant placed at a site that had alveolar ridge preservation three decades earlier. Infection at the site persisted 4 months after implant removal by flapless implant reversal. A flap was subsequently reflected, the grafted bone was removed, and a second alveolar ridge preservation was performed with a freeze-dried bone allograft. The publication reported infection resolved, and the site healed uneventfully. However, it is unknown if placement of another implant at the site would be successful. The purpose of this paper is to report on the findings at surgical reentry and outcome of the third implant.

Methods

Eleven months after the second alveolar ridge preservation, the site was reentered. The bone graft was found to be partially soft tissue encapsulated. All encapsulated graft materials and soft tissue were removed. An implant was placed, and the alveolar defect was grafted with a demineralized bone allograft. Seventeen months after implant placement, a buccal free gingival graft was performed during which the crestal bone adjacent to the implant was found to be hard and corticated. The implant was deemed to be osseointegrated and restored after soft tissue healing.

Results

Twenty-five months after implant placement, the third implant remained functional and asymptomatic with the peri-implant bone exhibiting normal trabeculation.

Conclusions

Implant therapy can be successful following treatment and resolution of perigraftitis.

Key points

  • Perigraftitis may play a contributing role in the biologic complications of implants that have been placed into grafted bone.

  • Perigraftitis may be successfully resolved by completely removing all grafted bone.

  • Once perigraftitis has been eliminated, an implant may be successfully placed.

背景:先前的一个病例研究报道了30年前在牙槽嵴保存的位置放置第二个种植体的种植周炎和伴发性种植周炎。在无瓣种植体反转移除种植体4个月后,该部位的感染持续存在。随后皮瓣反射,移植物骨被移除,并进行第二次牙槽嵴保存与冻干骨异体移植物。该出版物报道说,感染解决了,该部位平静地愈合了。然而,尚不清楚在该部位放置另一个植入物是否会成功。本文的目的是报告手术再入的发现和第三次种植的结果。方法:第二次牙槽嵴保存11个月后再入牙槽嵴。骨移植发现部分软组织被包裹。所有包封的移植物材料和软组织均被移除。放置种植体,用脱矿骨同种异体移植物移植牙槽缺损。种植体放置17个月后,进行了颊游离牙龈移植,期间发现种植体附近的冠骨坚硬且有皮质。种植体被认为是骨整合的,并在软组织愈合后恢复。结果:种植体放置25个月后,第三个种植体保持功能和无症状,种植体周围骨显示正常小梁。结论:种植体治疗可以成功治疗和解决积膜炎。关键点:骨周炎可能在植入骨内植入物的生物学并发症中起重要作用。通过完全去除所有移植骨,可以成功地解决骨周炎。一旦肩周炎被消除,植入物就可以成功放置。
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引用次数: 0
Alveolar ridge reconstruction with a digitally customized bone block allograft. 数字化定制骨块同种异体移植牙槽嵴重建。
IF 0.7 Q3 Medicine Pub Date : 2023-11-13 DOI: 10.1002/cap.10270
Hernán Bontá, Juliana Bugiolachi, Carla A Perrote, Luciana M Sánchez, Gisela E Pulitano Manisagian, Federico G Galli, Facundo Caride

Background: Reduced alveolar ridge volume is an often consequence after tooth loss, compromising implant placement and prosthetic rehabilitation. The digital customization of bone block allografts (BBA) is an alternative that incorporates advantages such as intimate contact with the recipient bed, increasing graft stability and reduced surgical time. In addition, enamel matrix derivate (EMD) has attracted interest for its effect on osteogenic gene expression and cell adhesion; few studies have focused on the benefits of bone regeneration with EMD. The aim of this case report is to present the reconstruction of a severely atrophic alveolar ridge defect with a digitally customized bone block allograft (CBBA) in combination with EMD as an adjuvant for bone regeneration and soft tissue healing.

Methods: Initially, the digital planning and manufacture of the BBA was performed based on an initial cone beam computed tomography (CBCT) scan. EMD was applied to the recipient site and to the CBBA before graft fixation. After 6 months, bone biopsies were obtained on re-entry surgery for prosthetically guided implant placement.

Results: Clinically, bone block showed good integration with the adjacent tissue and no signs of rejection or necrosis were found. On the histological evaluation, new bone was observed in intimate contact with the allograft and showed viable osteocytes and osteoblasts along its entire length. Residual allograft particles were observed to be highly osteoconductive.

Conclusion: According to the clinical and histological results presented, the digital customization of the BBA allows an ideal graft fit to the recipient bed with excellent results in bone regeneration.

背景:牙槽嵴体积减小通常是牙齿脱落后的后果,影响种植体的放置和修复。骨块同种异体移植物(BBA)的数字定制是一种替代方案,具有与受体床密切接触,增加移植物稳定性和缩短手术时间等优点。此外,釉质基质衍生物(EMD)因其对成骨基因表达和细胞粘附的影响而受到关注;很少有研究关注EMD对骨再生的益处。本病例报告的目的是介绍用数字定制骨块异体移植物(CBBA)结合EMD作为骨再生和软组织愈合的辅助来重建严重萎缩的牙槽嵴缺损。方法:首先,基于初始锥形束计算机断层扫描(CBCT)进行BBA的数字化规划和制造。在移植物固定前,将EMD应用于受体部位和CBBA。6个月后,在假体引导种植体再入手术时进行骨活检。结果:临床显示骨块与邻近组织融合良好,无排斥反应和坏死迹象。在组织学评估中,观察到新骨与同种异体移植物密切接触,并在其整个长度上显示有活力的骨细胞和成骨细胞。残余的同种异体移植物颗粒具有高度的骨导电性。结论:根据临床和组织学结果,数字定制的BBA可以使移植物与受体床理想贴合,并具有良好的骨再生效果。
{"title":"Alveolar ridge reconstruction with a digitally customized bone block allograft.","authors":"Hernán Bontá, Juliana Bugiolachi, Carla A Perrote, Luciana M Sánchez, Gisela E Pulitano Manisagian, Federico G Galli, Facundo Caride","doi":"10.1002/cap.10270","DOIUrl":"https://doi.org/10.1002/cap.10270","url":null,"abstract":"<p><strong>Background: </strong>Reduced alveolar ridge volume is an often consequence after tooth loss, compromising implant placement and prosthetic rehabilitation. The digital customization of bone block allografts (BBA) is an alternative that incorporates advantages such as intimate contact with the recipient bed, increasing graft stability and reduced surgical time. In addition, enamel matrix derivate (EMD) has attracted interest for its effect on osteogenic gene expression and cell adhesion; few studies have focused on the benefits of bone regeneration with EMD. The aim of this case report is to present the reconstruction of a severely atrophic alveolar ridge defect with a digitally customized bone block allograft (CBBA) in combination with EMD as an adjuvant for bone regeneration and soft tissue healing.</p><p><strong>Methods: </strong>Initially, the digital planning and manufacture of the BBA was performed based on an initial cone beam computed tomography (CBCT) scan. EMD was applied to the recipient site and to the CBBA before graft fixation. After 6 months, bone biopsies were obtained on re-entry surgery for prosthetically guided implant placement.</p><p><strong>Results: </strong>Clinically, bone block showed good integration with the adjacent tissue and no signs of rejection or necrosis were found. On the histological evaluation, new bone was observed in intimate contact with the allograft and showed viable osteocytes and osteoblasts along its entire length. Residual allograft particles were observed to be highly osteoconductive.</p><p><strong>Conclusion: </strong>According to the clinical and histological results presented, the digital customization of the BBA allows an ideal graft fit to the recipient bed with excellent results in bone regeneration.</p>","PeriodicalId":55950,"journal":{"name":"Clinical Advances in Periodontics","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92157422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Intraoral scanning and dental photogrammetry for full-arch implant-supported prosthesis: A technique. 全牙弓种植体支撑修复体的口腔内扫描和牙科摄影测量:一项技术。
IF 0.7 Q3 Medicine Pub Date : 2023-11-03 DOI: 10.1002/cap.10269
Emanuele Clozza

Objective: To introduce a novel digital workflow to replace a terminal or a complete edentulous arch with a complete-arch implant-supported fixed prosthesis.

Clinical considerations: A novel fully digital protocol provides an interim and definitive prosthesis by integrating extraoral dental photogrammetry with intraoral scanners.

Conclusions: The described technique has great potential to deliver a complete-arch implant-supported fixed interim and definitive prosthesis in edentulous patients in an efficient way.

Clinical significance: In the era of digital dentistry, using a fully digital workflow in full arch implant rehabilitations might be beneficial to replace multistep conventional methods.

Key points: Why is this case new information? To the author's knowledge, this article illustrates the first step-by-step technique that combines intraoral scanning with dental photogrammetry to provide a full-arch implant-supported interim and definitive prosthesis. What are the keys to successful management of this case? Well-defined presurgical planning and adequate primary stability of the dental implants. Accurate digital impressions of the scan bodies. A dental laboratory that is familiar with digital workflow for complete-arch rehabilitations. What are the primary limitations to success in this case? The learning curve of the scanning procedure. The cost barriers to acquire intraoral and photogrammetry scanners. Limited clinical studies that investigated the accuracy of intraoral scanning and dental photogrammetry for complete-arch digital implant impression.

目的:介绍一种新的数字化工作流程,用全牙弓种植体支撑的固定修复体代替末端或完全无牙弓。临床注意事项:一种新的全数字协议通过将口腔外牙科摄影测量与口腔内扫描仪相结合,提供了一种临时和最终的假体。结论:所描述的技术具有巨大的潜力,可以有效地为缺牙患者提供完整的足弓种植体支撑的固定临时和最终假体。临床意义:在数字牙科时代,在全弓种植体修复中使用全数字化工作流程可能有利于取代多步骤的传统方法。要点:为什么这个案例是新信息?据作者所知,本文介绍了第一种将口腔内扫描与牙科摄影测量相结合的分步技术,以提供全弓种植体支撑的临时和最终假体。成功管理此案的关键是什么?明确的术前计划和足够的牙科植入物的初步稳定性。扫描体的精确数字印象。一个熟悉完整足弓修复数字工作流程的牙科实验室。在这种情况下,成功的主要限制是什么?扫描过程的学习曲线。获取口腔内和摄影测量扫描仪的成本障碍。有限的临床研究调查了口腔内扫描和牙科摄影测量对全弓数字种植体印模的准确性。
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引用次数: 0
Human platelet-derived growth factor-BB (rhPDGF-BB) with collagen matrix for sinus elevation without using a bone graft. 人血小板衍生生长因子BB(rhPDGF-BB)与胶原基质用于窦性抬高,而不使用骨移植物。
IF 0.7 Q3 Medicine Pub Date : 2023-10-23 DOI: 10.1002/cap.10268
Iya H Ghassib, Muhammad H A Saleh, Hom-Lay Wang

Background: This case report involves a 38-year-old male who presented to the clinic after experiencing complications from a tooth extraction, including a dislodged root segment in the sinus, a sinus wall fenestration on the palate, a residual bone height (RBH) of 3 mm, and inadequate healing of the soft tissue. He presented for implant placement.

Methods: Recombinant human platelet-derived growth factor-BB (rh-PDGF-BB) was applied to a wound dressing material and placed in the sinus cavity alongside a 4.8 × 10 mm dental implant (Straumann SP, Straumann, Andover, MA, USA.). As documented in the literature, a graftless sinus lift via a lateral window was performed using a split-thickness flap technique to elevate the sinus membrane, re-establish its integrity, and restore its barrier function.

Results: An 8-month cone beam computed tomography assessment showed a 6.2 mm vertical bone gain and complete implant coverage.

Conclusion: This approach provided a successful alternative to shorten treatment duration and achieve favorable radiographic outcomes during early healing.

Key points: The use of rhPDGF-BB and a collagen matrix in a sinus lift procedure emerges as a practical therapeutic option when grafting might lack predictability and notably consume more treatment time, while also achieving the desired bone height when used with a simultaneously placed implant.

背景:本病例报告涉及一名38岁男性,他在经历了拔牙并发症后来到诊所,包括鼻窦根段移位、腭部窦壁开窗、残余骨高(RBH)为3毫米和软组织愈合不充分。他提出植入。方法:将重组人血小板衍生生长因子BB(rh-PDGF-BB)应用于伤口敷料材料上,并与4.8×10mm的牙科植入物(Straumann SP,Straumann,Andover,MA,USA)一起放置在窦腔内。如文献所述,通过侧窗进行无移植物窦提举,使用分瓣技术来抬高窦膜,重建其完整性并恢复其屏障功能。结果:一项为期8个月的锥形束计算机断层扫描评估显示,垂直骨增加了6.2mm,植入物完全覆盖。结论:该方法为缩短治疗时间和在早期愈合过程中获得良好的放射学结果提供了一种成功的替代方案。要点:当移植可能缺乏可预测性,并且明显消耗更多的治疗时间,同时与同时放置的植入物一起使用时,在鼻窦提拉手术中使用rhPDGF BB和胶原基质是一种实用的治疗选择。
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引用次数: 0
Anatomy-driven complexity classification for soft-tissue tunneling procedures 软组织隧道手术的解剖学驱动的复杂性分类。
IF 0.9 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2023-10-09 DOI: 10.1002/cap.10267
Amanda B. Rodriguez, Hsun-Liang Chan, Diego Velasquez

Background

The tunnel technique (TUN) preserves the integrity of the papilla by creating envelope flaps that allow for the insertion of a connective tissue graft, and/or biomaterials.

Methods

(1) A comprehensive overview of tunneling flap procedures in the treatment of gingival recessions (GRs) for soft tissue coverage is presented and (2) A classification system for soft and hard tissue anatomy of GR sites which may aid the clinician in determining the surgical complexity is being introduced.

Results

A novel clinical classification system is proposed to illustrate complexity levels determined by soft and hard tissue anatomy of GR sites such as the mucogingival junction proximity to the gingival margin, bone morphotype, and mucosal margin thickness.

Conclusions

TUN is highly effective in treating single/multiple GRs. Its limitations are related to variability in surgical site anatomy and operator expertise. A classification system based on anatomical soft and hard tissue variations has been proposed to help identify complexity levels encountered during tunneling procedures.

Key points

  1. Site-related factors directly impact the surgical variables related to tissue trauma, flap tension, soft tissue management, muscle pull, and wound stability during the healing of gingival recessions (GRs).

  2. The achievement of expedited and favorable wound healing is crucial to obtaining quantitative and qualitative success in the treatment of GR and the long-term stability of root coverage.

  3. A classification system based on anatomical soft and hard tissue variations has been proposed to facilitate tunneling procedures while respecting surgical principles.

背景:隧道技术(TUN)通过创建允许插入结缔组织移植物和/或生物材料的包膜瓣来保持乳头的完整性。方法:(1)全面综述了隧道皮瓣在治疗软组织覆盖的牙龈退缩(GR)中的应用。(2)介绍了一种牙龈退缩部位软组织和硬组织解剖的分类系统,该系统可以帮助临床医生确定手术的复杂性。结果:提出了一种新的临床分类系统来说明GR部位的软组织和硬组织解剖所确定的复杂性水平,如靠近牙龈边缘的粘牙龈交界处、骨形态类型和粘膜边缘厚度。结论:TUN治疗单一/多发性GRs疗效确切。其局限性与手术部位解剖和操作员专业知识的可变性有关。已经提出了一种基于解剖软组织和硬组织变化的分类系统,以帮助识别隧道手术过程中遇到的复杂性水平。要点:在牙龈退缩(GRs)的愈合过程中,与部位相关的因素直接影响与组织创伤、皮瓣张力、软组织管理、肌肉牵拉和伤口稳定性相关的手术变量。实现快速和有利的伤口愈合对于在GR治疗中获得定量和定性的成功以及根部覆盖的长期稳定性至关重要。已经提出了一种基于解剖软组织和硬组织变化的分类系统,以促进隧道手术,同时尊重手术原则。
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引用次数: 0
Clinical evaluation of the combined efficacy of injectable platelet-rich fibrin along with scaling and root planing in the non-surgical periodontal therapy of stage III and grade C periodontitis patients having type 2 diabetes mellitus: A randomized controlled trial 注射用富含血小板的纤维蛋白与洁治和根刨在2型糖尿病III期和C级牙周炎患者非手术牙周治疗中的联合疗效的临床评估:一项随机对照试验。
IF 0.9 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2023-09-25 DOI: 10.1002/cap.10266
Prasanth D Shunmuga, Anupama Tadepalli, Harinath Parthasarathy, Deepa Ponnaiyan, Priyanka k Cholan, Lakshmi Ramachandran
<div> <section> <h3> Background</h3> <p>This randomized controlled trial was aimed to evaluate the combined efficacy of injectable platelet-rich fibrin (i-PRF) and scaling and root planing (SRP) in type 2 diabetes mellitus subjects having periodontitis.</p> </section> <section> <h3> Methods</h3> <p>Twenty-six Stage III, grade C periodontitis subjects (HbA1c > 7) were recruited in this split-mouth study. Following SRP, the test sites received subgingival application of i-PRF, while the control sites received saline. Plaque index, bleeding on probing, modified gingival index, probing pocket depth (PPD) and clinical attachment level (CAL) were measured at baseline, 3 and 6 months.</p> </section> <section> <h3> Results</h3> <p>Twenty-three participants completed the course of research. The Friedman test followed by Dunn's post hoc test, revealed significant improvement in all the clinical parameters from baseline to 3 and 6 months in both the study groups (<i>p</i> ≤ 0.05). The mean PPD and CAL decreased from 6.30 ± 1.25 and 7.48 ± 1.75 at baseline to 3.48 ± 1.34 and 4.39 ± 1.67 at 6 months in control sites and from 6.57 ± 1.56 and 7.61 ± 1.69 to 3.39 ± 1.23 and 4.26 ± 1.81 at 6 months in test sites (<i>p</i> ≤ 0.0001). Intergroup analysis found no statistical significant differences in the evaluated parameters across all time intervals (<i>p</i> > 0.05)</p> </section> <section> <h3> Conclusion</h3> <p>The results indicated that the adjunctive application of i-PRF to SRP provided similar benefits as saline and SRP in diabetes mellitus subjects.</p> </section> <section> <h3> Key points</h3> <div> <ol> <li> <p>Question: To find the combined efficacy of injectable platelet-rich fibrin (i-PRF) along with scaling and root planing (SRP) in the management of periodontal pockets of Stage III and Grade C periodontitis patients having Type 2 Diabetes Mellitus (T2DM).</p> </li> <li> <p>Finding: All of the treated sites showed satisfactory healing. Both the treatment modalities (i-PRF + SRP and Saline + SRP) were effective in the treatment of periodontal pockets. At 3 and 6 months, there were no significant differences in periodontal parameters between groups.</p> </li> <li> <p>Meaning: The application of i-PRF as an adjunct to SRP provided similar benefits as saline and SRP in improving clinical parameters in subjects with stage III
背景:这项随机对照试验旨在评估注射用富含血小板的纤维蛋白(i-PRF)和洁治和牙根刨削(SRP)对患有牙周炎的2型糖尿病受试者的联合疗效。方法:在这项分口研究中招募了26名III期C级牙周炎受试者(HbA1c>7)。SRP后,试验部位接受龈下i-PRF应用,而对照部位接受生理盐水。在基线、3个月和6个月时测量牙菌斑指数、探查出血量、改良牙龈指数、探查袋深度(PPD)和临床附着水平(CAL)。结果:23名参与者完成了研究课程。Friedman检验之后是Dunn的post-hoc检验,结果显示,从基线到3个月和6个月,两个研究组的所有临床参数都有显著改善(p≤0.05)。对照组的平均PPD和CAL从基线时的6.30±1.25和7.48±1.75降至6个月时的3.48±1.34和4.39±1.67,试验组从6.57±1.56和7.61±1.69降至3.39±1.23和4.26±1.81(p≤0.0001)。组间分析发现,在所有时间间隔内,评估参数没有统计学上的显著差异(p>0.05)结论:结果表明,i-PRF对SRP的辅助应用在糖尿病受试者中提供了与生理盐水和SRP相似的益处。要点:问题:寻找注射富含血小板的纤维蛋白(i-PRF)与刮治和根刨(SRP)在治疗患有2型糖尿病(T2DM)的III期和C级牙周炎患者的牙周袋中的联合疗效。发现:所有治疗部位都显示出满意的愈合。两种治疗方式(i-PRF+SRP和Saline+SRP)均能有效治疗牙周袋。在3个月和6个月时,两组之间的牙周参数没有显著差异。意义:在患有T2DM(HbA1C>7)的III期和C级牙周炎患者中,应用i-PRF作为SRP的辅助药物在改善临床参数方面提供了与生理盐水和SRP类似的益处。
{"title":"Clinical evaluation of the combined efficacy of injectable platelet-rich fibrin along with scaling and root planing in the non-surgical periodontal therapy of stage III and grade C periodontitis patients having type 2 diabetes mellitus: A randomized controlled trial","authors":"Prasanth D Shunmuga,&nbsp;Anupama Tadepalli,&nbsp;Harinath Parthasarathy,&nbsp;Deepa Ponnaiyan,&nbsp;Priyanka k Cholan,&nbsp;Lakshmi Ramachandran","doi":"10.1002/cap.10266","DOIUrl":"10.1002/cap.10266","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Background&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;This randomized controlled trial was aimed to evaluate the combined efficacy of injectable platelet-rich fibrin (i-PRF) and scaling and root planing (SRP) in type 2 diabetes mellitus subjects having periodontitis.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Twenty-six Stage III, grade C periodontitis subjects (HbA1c &gt; 7) were recruited in this split-mouth study. Following SRP, the test sites received subgingival application of i-PRF, while the control sites received saline. Plaque index, bleeding on probing, modified gingival index, probing pocket depth (PPD) and clinical attachment level (CAL) were measured at baseline, 3 and 6 months.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Results&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Twenty-three participants completed the course of research. The Friedman test followed by Dunn's post hoc test, revealed significant improvement in all the clinical parameters from baseline to 3 and 6 months in both the study groups (&lt;i&gt;p&lt;/i&gt; ≤ 0.05). The mean PPD and CAL decreased from 6.30 ± 1.25 and 7.48 ± 1.75 at baseline to 3.48 ± 1.34 and 4.39 ± 1.67 at 6 months in control sites and from 6.57 ± 1.56 and 7.61 ± 1.69 to 3.39 ± 1.23 and 4.26 ± 1.81 at 6 months in test sites (&lt;i&gt;p&lt;/i&gt; ≤ 0.0001). Intergroup analysis found no statistical significant differences in the evaluated parameters across all time intervals (&lt;i&gt;p&lt;/i&gt; &gt; 0.05)&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Conclusion&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;The results indicated that the adjunctive application of i-PRF to SRP provided similar benefits as saline and SRP in diabetes mellitus subjects.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Key points&lt;/h3&gt;\u0000 \u0000 &lt;div&gt;\u0000 &lt;ol&gt;\u0000 \u0000 &lt;li&gt;\u0000 &lt;p&gt;Question: To find the combined efficacy of injectable platelet-rich fibrin (i-PRF) along with scaling and root planing (SRP) in the management of periodontal pockets of Stage III and Grade C periodontitis patients having Type 2 Diabetes Mellitus (T2DM).&lt;/p&gt;\u0000 &lt;/li&gt;\u0000 \u0000 &lt;li&gt;\u0000 &lt;p&gt;Finding: All of the treated sites showed satisfactory healing. Both the treatment modalities (i-PRF + SRP and Saline + SRP) were effective in the treatment of periodontal pockets. At 3 and 6 months, there were no significant differences in periodontal parameters between groups.&lt;/p&gt;\u0000 &lt;/li&gt;\u0000 \u0000 &lt;li&gt;\u0000 &lt;p&gt;Meaning: The application of i-PRF as an adjunct to SRP provided similar benefits as saline and SRP in improving clinical parameters in subjects with stage III ","PeriodicalId":55950,"journal":{"name":"Clinical Advances in Periodontics","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41161829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Operating microscope-assisted reconstructive strategy for peri-implantitis: A case series report 种植体周围炎的手术显微镜辅助重建策略:一例系列报告。
IF 0.9 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2023-09-19 DOI: 10.1002/cap.10265
Yi-Chen Chiang, Benyapha Sirinirund, Amanda Rodriguez, Diego Velasquez, Hsun-Liang Chan

Background

Treating peri-implantitis with reconstructive means has been largely unpredictable due to access limitations for surface decontamination, unfavorable bony topography, difficulty in achieving wound stability, and inferior soft tissue qualities. A microsurgical approach with the use of the operating microscope (OM) that provides adjustable higher magnification (∼5–30 times) and coaxial illumination, coupled with the use of microsurgical instruments, may overcome, or alleviate some of the abovementioned obstacles, resulting in more predictable outcomes.

Methods

Three patients received reconstructive therapy for correcting peri-implant defects under OM in private practice settings. After precise incisions to preserve soft tissue volume, the flaps were dissected prudently from underlying granulomatous tissues, which were subsequently removed, followed by controlled flap releasing under ∼10–15x magnification. Surface decontamination was performed using a piezoelectric ultrasonic device, air polishing, and hand instruments at ∼30x magnification. The biomaterial selections were dehydrated human de-epithelialized amnion-chorion membrane with mineralized allograft particulates in two cases and xenografts in one case, based on the surgeons’ preference. Wound closure followed the non-submerged approach.

Results

These cases demonstrated uneventful soft tissue healing, favorable radiographic bone fill, and disease resolution with follow-ups ranging from 2 to 4 years.

Conclusions

Preliminary data suggest encouraging outcomes after the microsurgical approach following biological as well as biomechanical principles for peri-implant defect reconstruction.

背景:由于表面去污的途径限制、不利的骨地形、难以实现伤口稳定性和较差的软组织质量,用重建方法治疗种植体周围炎在很大程度上是不可预测的。使用手术显微镜(OM)提供可调节的更高放大率(~5-30倍)和同轴照明的显微外科方法,再加上使用显微外科仪器,可以克服或减轻上述一些障碍,从而产生更可预测的结果。方法:三名患者在私人诊所接受OM下修复种植体周围缺陷的重建治疗。在精确切割以保持软组织体积后,从下方的肉芽肿组织中谨慎地解剖皮瓣,随后将其移除,然后在约10-15倍的放大倍数下控制皮瓣释放。表面去污使用压电超声波设备、空气抛光和手动仪器进行,放大倍数为~30倍。根据外科医生的偏好,生物材料的选择是两例带有矿化同种异体移植物颗粒的脱水人去上皮化羊膜绒毛膜和一例异种移植物。伤口闭合采用非水下入路。结果:这些病例表现出平稳的软组织愈合、良好的放射学骨填充和疾病的解决,随访时间为2-4年。结论:初步数据表明,根据生物学和生物力学原理,显微外科手术后植入物周围缺损重建的结果令人鼓舞。
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引用次数: 0
Protocol for ridge preservation at severely compromised extraction sockets: Consecutive case series 严重受损拔牙窝的牙脊保存方案连续病例系列
IF 0.7 Q3 Medicine Pub Date : 2023-08-09 DOI: 10.1002/cap.10263
Aaron N. Colamarino, William E. Bane, Gary M. Blyleven, Adam R. Lincicum, Brian W. Stancoven, Thomas M. Johnson

Background

The physiologic bone remodeling accompanying tooth extraction is a phenomenon well described in the dental literature. Extraction sockets severely compromised by local infection, trauma, iatrogenesis, or other factors may exhibit enhanced reduction in alveolar dimensions during healing. The purpose of this report is to present an alveolar ridge preservation (ARP) protocol specifically intended for use at severely compromised sites.

Methods

Seven patients presented to the Department of Periodontics, Army Postgraduate Dental School, Fort Gordon, Georgia, requiring extraction of teeth with partial or near-complete loss of the facial/buccal cortex. At each site, a cross-linked bovine collagen membrane was used to prevent collapse of the facial/buccal soft tissue and maintain space, a freeze-dried bone allograft was applied in the socket, and a dense polytetrafluoroethylene membrane covered the occlusal aspect.

Results

All sites healed uneventfully and resulted in favorable alveolar ridge dimensions for implant placement.

Conclusion

Few authors have proposed specific ARP methods for managing severely deficient extraction sockets. The predominant recommendation has been staged reconstruction of the site applying hard and soft tissue augmentation. Observations reported herein suggest that staged reconstruction is avoidable at some extraction sockets exhibiting severe alveolar compromise. Controlled clinical investigation of this protocol appears warranted.

Key points

  • Few authors have proposed alveolar ridge preservation (ARP) methods specifically intended for use at severely compromised extraction sockets.
  • The prevailing recommendation at such sites is a staged protocol involving tooth extraction with delayed hard and soft tissue augmentation.
  • The presented bilaminar ARP technique may eliminate the need for staged reconstruction at some severely compromised extraction sockets.
背景 拔牙后的生理性骨重塑是牙科文献中描述较多的一种现象。由于局部感染、外伤、先天性因素或其他因素而严重受损的拔牙窝在愈合过程中可能会表现出牙槽骨尺寸的进一步缩小。本报告旨在介绍一种专门用于严重受损部位的牙槽嵴保留(ARP)方案。 方法 七名患者来到佐治亚州戈登堡陆军研究生牙科学院牙周病学系,需要拔除面部/颊部皮质部分或接近完全丧失的牙齿。在每个部位都使用了交联牛胶原膜,以防止面部/颊部软组织塌陷并保持空间,在牙槽窝内应用了冻干骨异体移植,并在咬合面上覆盖了一层致密的聚四氟乙烯膜。 结果 所有部位均顺利愈合,牙槽嵴的尺寸有利于种植体的植入。 结论 对于严重缺损的拔牙窝,很少有学者提出具体的 ARP 方法。最主要的建议是应用硬组织和软组织增量分阶段重建拔牙窝。本文的观察结果表明,在一些牙槽骨严重受损的拔牙窝,分阶段重建是可以避免的。看来有必要对这一方案进行临床对照研究。 要点 很少有学者提出专门用于严重受损拔牙窝的牙槽嵴保留(ARP)方法。 在这种情况下,普遍的建议是采用分阶段的方案,包括拔牙和延迟的硬组织和软组织增量。 所介绍的双层牙槽嵴ARP技术可使某些严重受损的拔牙窝不再需要分阶段重建。
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引用次数: 0
期刊
Clinical Advances in Periodontics
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