Pub Date : 2018-07-10DOI: 10.5073/JABFQ.2018.091.021
Xiamin Cao, Yongtao Wang, X. Liao, Xiaosong Hu
The physico-chemical and bio-chemical compositions of Hongyan, Tiangxiang, Tongzi Ι and Zhangji strawberries inChinawere analyzed. Their values were pH 3.42~3.73, titration acidity 0.63~0.79%, total soluble sugars 5.26~8.95 g/100 gfresh weight (FW), ascorbic acid 21.38~42.89 mg/100 gFW, total phenolics 235.12~444.73 mg/100 gFW, pectin 82.84~96.13 mg/100 gFW, total organic acids 874.30~1216.27 mg/100 gFW, Individual phenolic compounds other than anthocyanins 7.60~12.18 mg/100 gFW, free amino acids 13.35~32.66 mg/100 gFW, monomeric anthocyanins 4.47~47.19 mg/100gFW, antioxidant capacity of ·DPPH 14.14~18.87 and FRAP 7.97~10.54 equal to mg/100 gVc, polyphenol oxidase (PPO) activity 0~0.42 Abs/min, peroxidase (POD) activity 0.17~0.34 Abs/min and pectin methyl esterase (PME) activity 0.012~0.018 mL/min. Tongzi Ι was most suitable for food processing due to the highest titration acidity, total phenolics, pectin, total organic acids, monomeric anthocyanins, antioxidant capacity of ·DPPH and FRAP with lower PPO, POD and PME activity.
{"title":"Characterization of physico-chemical and bio-chemical compositions of selected four strawberry cultivars","authors":"Xiamin Cao, Yongtao Wang, X. Liao, Xiaosong Hu","doi":"10.5073/JABFQ.2018.091.021","DOIUrl":"https://doi.org/10.5073/JABFQ.2018.091.021","url":null,"abstract":"The physico-chemical and bio-chemical compositions of Hongyan, Tiangxiang, Tongzi Ι and Zhangji strawberries inChinawere analyzed. Their values were pH 3.42~3.73, titration acidity 0.63~0.79%, total soluble sugars 5.26~8.95 g/100 gfresh weight (FW), ascorbic acid 21.38~42.89 mg/100 gFW, total phenolics 235.12~444.73 mg/100 gFW, pectin 82.84~96.13 mg/100 gFW, total organic acids 874.30~1216.27 mg/100 gFW, Individual phenolic compounds other than anthocyanins 7.60~12.18 mg/100 gFW, free amino acids 13.35~32.66 mg/100 gFW, monomeric anthocyanins 4.47~47.19 mg/100gFW, antioxidant capacity of ·DPPH 14.14~18.87 and FRAP 7.97~10.54 equal to mg/100 gVc, polyphenol oxidase (PPO) activity 0~0.42 Abs/min, peroxidase (POD) activity 0.17~0.34 Abs/min and pectin methyl esterase (PME) activity 0.012~0.018 mL/min. Tongzi Ι was most suitable for food processing due to the highest titration acidity, total phenolics, pectin, total organic acids, monomeric anthocyanins, antioxidant capacity of ·DPPH and FRAP with lower PPO, POD and PME activity.","PeriodicalId":56276,"journal":{"name":"Journal of Applied Botany and Food Quality-Angewandte Botanik","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2018-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46347682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The extraction conditions of epigallocatechin-3-gallate (EGCG) from Jinxuan summer green tea and antitumor activity against human gastric cancer SGC-7901 cells of the green tea extracts were investigated. On the basis of a single factor experiment, Box-Behnken design and response surface methodology were employed to optimize the hot water extraction conditions. The optimal extraction conditions for EGCG were determined as: extraction temperature of 85 °C, extraction time of 34 min, water-tea ratio of 41 mL/g, a solution of pH 6, and extraction twice. Under these conditions, the experimental extraction yield value of EGCG was 33.82 mg/g, which was not significantly different in comparison to predicted values. The results indicated that the regression models were suitable for the EGCG extraction from Jinxuan summer green tea. The summer green tea extract prepared under the optimal conditions had a higher antitumor activity against human gastric cancer SGC-7901 cells than the green tea extract made with traditional tea brewing method.
{"title":"Optimization of brewing conditions in epigallocatechin-3-gallate (EGCG) extraction from Jinxuan summer green tea by response surface methodology","authors":"Xiang Limin, Shunshun Pan, Xingfei Lai, Lingli Sun, Zhigang Li, Qiuhua Li, Ya-hui Huang, Sun Shili","doi":"10.5073/JABFQ.2018.091.022","DOIUrl":"https://doi.org/10.5073/JABFQ.2018.091.022","url":null,"abstract":"The extraction conditions of epigallocatechin-3-gallate (EGCG) from Jinxuan summer green tea and antitumor activity against human gastric cancer SGC-7901 cells of the green tea extracts were investigated. On the basis of a single factor experiment, Box-Behnken design and response surface methodology were employed to optimize the hot water extraction conditions. The optimal extraction conditions for EGCG were determined as: extraction temperature of 85 °C, extraction time of 34 min, water-tea ratio of 41 mL/g, a solution of pH 6, and extraction twice. Under these conditions, the experimental extraction yield value of EGCG was 33.82 mg/g, which was not significantly different in comparison to predicted values. The results indicated that the regression models were suitable for the EGCG extraction from Jinxuan summer green tea. The summer green tea extract prepared under the optimal conditions had a higher antitumor activity against human gastric cancer SGC-7901 cells than the green tea extract made with traditional tea brewing method.","PeriodicalId":56276,"journal":{"name":"Journal of Applied Botany and Food Quality-Angewandte Botanik","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2018-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48316868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-11DOI: 10.5073/JABFQ.2018.091.019
D. Kulus
Tomato is one of the most popular vegetable crops. However, over time, the species has suffered a strong genetic diversity reduction and domestication bottlenecking. This growing trend is known as the genetic erosion. The human intervention on the genetic erosion intensification is high and has severe implications on the future programmes of management and use of S. lycopersicum biodiversity. The wild tomato species (especially accessions originating from the Andes to Mesoamerica) harbour many valuable genes, which have been lost among the cultivated ones. Therefore, there is an increasing interest to mine new alleles from the interspecific gene pool of Lycopersicon section. Sustainable genetic diversity management constitutes a basis for crop improvement, classification and protection. Moreover, conservation of plant genetic resources is crucial to food security, as well as pharmaceutical industry. There are a few strategies developed which address the preservation of tomato genetic resources. In situ and ex situ conservation are the two main complementary methods of biodiversity protection. The aim of this review is to summarise the most recent information about tomato genetic resources, genetic erosion phenomenon, as well as some traditional and modern preservation strategies.
{"title":"Genetic resources and selected conservation methods of tomato","authors":"D. Kulus","doi":"10.5073/JABFQ.2018.091.019","DOIUrl":"https://doi.org/10.5073/JABFQ.2018.091.019","url":null,"abstract":"Tomato is one of the most popular vegetable crops. However, over time, the species has suffered a strong genetic diversity reduction and domestication bottlenecking. This growing trend is known as the genetic erosion. The human intervention on the genetic erosion intensification is high and has severe implications on the future programmes of management and use of S. lycopersicum biodiversity. The wild tomato species (especially accessions originating from the Andes to Mesoamerica) harbour many valuable genes, which have been lost among the cultivated ones. Therefore, there is an increasing interest to mine new alleles from the interspecific gene pool of Lycopersicon section. Sustainable genetic diversity management constitutes a basis for crop improvement, classification and protection. Moreover, conservation of plant genetic resources is crucial to food security, as well as pharmaceutical industry. There are a few strategies developed which address the preservation of tomato genetic resources. In situ and ex situ conservation are the two main complementary methods of biodiversity protection. The aim of this review is to summarise the most recent information about tomato genetic resources, genetic erosion phenomenon, as well as some traditional and modern preservation strategies.","PeriodicalId":56276,"journal":{"name":"Journal of Applied Botany and Food Quality-Angewandte Botanik","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2018-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44535906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-11DOI: 10.5073/JABFQ.2018.091.018
S. Duletić-Laušević, Ana Z. Alimpić, S. Kolarević, B. Vuković-Gačić, Mariana M Oalđe, J. Živković, K. Šavikin, P. Marin
The aim of this study was to examine chemical composition, as well as antineurodegenerative, antioxidant and antibacterial activity of aqueous and ethanolic extracts of Origanum majorana L. (Lamiaceae) originating from Serbia, Greece, Egypt and Libya. Total phenolics and flavonoids, antioxidant activities, and acetylcholinesterase and tyrosinase inhibitory activities were measured spectrophotometrically. Determination of phenolic compounds in extracts was done using HPLC-DAD technique. Antibacterial activity included determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using the microdilution method. The highest phenolic and flavonoid contents were recorded in the ethanolic extract of the Egyptian sample and in aqueous extract of Serbian sample. The HPLC analysis showed high content of rosmarinic acid, with the highest amount found in the ethanolic extract of the plants from Egypt. Water extracts showed prevalently better antioxidant and antineurodegenerative activity in applied tests than the ethanolic extracts. Gram-positive bacterial strains showed higher sensitivity to tested extracts. According to the obtained results, sweet marjoram samples from Serbia and Egypt can be marked as more promising, due to the highest content of total phenolics and flavonoids and the best antioxidant, antibacterial and tyrosinase inhibitory activity.
{"title":"Antineurodegenerative, antioxidant and antibacterial activities and phenolic components of Origanum majorana L. (Lamiaceae) extracts of different origin","authors":"S. Duletić-Laušević, Ana Z. Alimpić, S. Kolarević, B. Vuković-Gačić, Mariana M Oalđe, J. Živković, K. Šavikin, P. Marin","doi":"10.5073/JABFQ.2018.091.018","DOIUrl":"https://doi.org/10.5073/JABFQ.2018.091.018","url":null,"abstract":"The aim of this study was to examine chemical composition, as well as antineurodegenerative, antioxidant and antibacterial activity of aqueous and ethanolic extracts of Origanum majorana L. (Lamiaceae) originating from Serbia, Greece, Egypt and Libya. Total phenolics and flavonoids, antioxidant activities, and acetylcholinesterase and tyrosinase inhibitory activities were measured spectrophotometrically. Determination of phenolic compounds in extracts was done using HPLC-DAD technique. Antibacterial activity included determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using the microdilution method. The highest phenolic and flavonoid contents were recorded in the ethanolic extract of the Egyptian sample and in aqueous extract of Serbian sample. The HPLC analysis showed high content of rosmarinic acid, with the highest amount found in the ethanolic extract of the plants from Egypt. Water extracts showed prevalently better antioxidant and antineurodegenerative activity in applied tests than the ethanolic extracts. Gram-positive bacterial strains showed higher sensitivity to tested extracts. According to the obtained results, sweet marjoram samples from Serbia and Egypt can be marked as more promising, due to the highest content of total phenolics and flavonoids and the best antioxidant, antibacterial and tyrosinase inhibitory activity.","PeriodicalId":56276,"journal":{"name":"Journal of Applied Botany and Food Quality-Angewandte Botanik","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2018-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46821107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-29DOI: 10.5073/JABFQ.2018.091.017
M. Dinu, R. Soare, C. Babeanu, G. Hoza
Two cultivars of sweet potatoes, Pumpkin and Chestnut, were planted in the didactic field of the Faculty of Horticulture, Craiova, and biochemical determinations were carried out from the blades and petioles of the leaves. This study highlights the antioxidant properties of these species, which are not well known in Romania, to encourage consumption of the vegetative parts of the plant, as well as use of the leaves in the future to develop natural antioxidants. The chlorophyll and carotene content in the blade and petiole were determined, highlighting the large amounts of petiole, depending on the cultivar. Catalase (CAT), peroxidase (POX), and vitamin C content, as well as total dry matter (TDM), reducing sugars, phenolic compounds, and antioxidant activity, were also measured. The petioles exhibited high antioxidant activity, with 62.186 μmol TE/g FW in Pumpkin and 95.168 μmol TE/g FW in Chestnut. The cultivars also exhibited differences at the blade level, with high values recorded for the Chestnut cultivar. Positive correlations between total polyphenols and reducing sugars (r = 0.89), antioxidant activity and reducing sugars (r = 0.48), and antioxidant activity and phenolic compounds (r = 0.53) were found. This study demonstrates that sweet potato leaves are a potential, inexpensive and beneficial source of natural antioxidants.
{"title":"Analysis of nutritional composition and antioxidant activity of sweet potato (Ipomoea batatas L.) leaf and petiole","authors":"M. Dinu, R. Soare, C. Babeanu, G. Hoza","doi":"10.5073/JABFQ.2018.091.017","DOIUrl":"https://doi.org/10.5073/JABFQ.2018.091.017","url":null,"abstract":"Two cultivars of sweet potatoes, Pumpkin and Chestnut, were planted in the didactic field of the Faculty of Horticulture, Craiova, and biochemical determinations were carried out from the blades and petioles of the leaves. This study highlights the antioxidant properties of these species, which are not well known in Romania, to encourage consumption of the vegetative parts of the plant, as well as use of the leaves in the future to develop natural antioxidants. The chlorophyll and carotene content in the blade and petiole were determined, highlighting the large amounts of petiole, depending on the cultivar. Catalase (CAT), peroxidase (POX), and vitamin C content, as well as total dry matter (TDM), reducing sugars, phenolic compounds, and antioxidant activity, were also measured. The petioles exhibited high antioxidant activity, with 62.186 μmol TE/g FW in Pumpkin and 95.168 μmol TE/g FW in Chestnut. The cultivars also exhibited differences at the blade level, with high values recorded for the Chestnut cultivar. Positive correlations between total polyphenols and reducing sugars (r = 0.89), antioxidant activity and reducing sugars (r = 0.48), and antioxidant activity and phenolic compounds (r = 0.53) were found. This study demonstrates that sweet potato leaves are a potential, inexpensive and beneficial source of natural antioxidants.","PeriodicalId":56276,"journal":{"name":"Journal of Applied Botany and Food Quality-Angewandte Botanik","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2018-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43411084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-16DOI: 10.5073/JABFQ.2018.091.016
Jin-Ge Zhao, Yu-Qing Zhang
Mulberry leaves have been widely used to produce various health products. In this paper, an efficient procedure of ultrasound-assisted hydroalcohol-acid extraction (UAHAE) was established to estimate the total flavonoids content in mulberry leaves by quantifying their resulting aglycones (quercetin and kaempferol) using HPLC-DAD. Effective hydrolysis of glycosides to aglycones was achieved in an ethanol-HCl-water (7/2/1, v/v/v) solution at 75°C by ultrasound (40 kHz) for 60 min. The average contents of quercetin and kaempferol were 7.12 mg/g and 2.13 mg/g, respectively, in the variety of cultivar MC308, and their recoveries were 105.48% and 105.81%, respectively. The total flavonoid content of most varieties was between 4-10 mg/g, accounting for 80% in 86 species of mulberry leaves. With the increase in leaf maturity, the general trend of the change in flavonoid content was a decrease at first and then an increase. The highest total flavonoid content in commercial mulberry tea (CT 2) is 4.76 mg/g, and the DPPH radical scavenging activities were similar to the distribution of total flavonoids measured by UAHAE in three types of mulberry tea. These finding provide a basis for industrial-scale manufacturing of edible medicinal mulberry leaf products.
{"title":"A novel estimation method of total flavonoids in an edible medicinal mulberry leaves by ultrasound-assisted hydroalcohol-acid extraction and HPLC-DAD","authors":"Jin-Ge Zhao, Yu-Qing Zhang","doi":"10.5073/JABFQ.2018.091.016","DOIUrl":"https://doi.org/10.5073/JABFQ.2018.091.016","url":null,"abstract":"Mulberry leaves have been widely used to produce various health products. In this paper, an efficient procedure of ultrasound-assisted hydroalcohol-acid extraction (UAHAE) was established to estimate the total flavonoids content in mulberry leaves by quantifying their resulting aglycones (quercetin and kaempferol) using HPLC-DAD. Effective hydrolysis of glycosides to aglycones was achieved in an ethanol-HCl-water (7/2/1, v/v/v) solution at 75°C by ultrasound (40 kHz) for 60 min. The average contents of quercetin and kaempferol were 7.12 mg/g and 2.13 mg/g, respectively, in the variety of cultivar MC308, and their recoveries were 105.48% and 105.81%, respectively. The total flavonoid content of most varieties was between 4-10 mg/g, accounting for 80% in 86 species of mulberry leaves. With the increase in leaf maturity, the general trend of the change in flavonoid content was a decrease at first and then an increase. The highest total flavonoid content in commercial mulberry tea (CT 2) is 4.76 mg/g, and the DPPH radical scavenging activities were similar to the distribution of total flavonoids measured by UAHAE in three types of mulberry tea. These finding provide a basis for industrial-scale manufacturing of edible medicinal mulberry leaf products.","PeriodicalId":56276,"journal":{"name":"Journal of Applied Botany and Food Quality-Angewandte Botanik","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2018-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42796376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-02DOI: 10.5073/JABFQ.2018.091.015
Yu Dong, H. Zhi
This study was to determine whether postharvest H2S fumigation effects surface pitting development of ‘Lapins’ and ‘Regina’ cherries after cold storage, and if so, how H2S takes participate in minimizing pitting injury and its relation to cell wall metabolism. Fruit were exposed to hydrogen sulfide (H2S) gas released from sodium hydrosulfide (NaHS at rates of 0.5, 1, 2, or 3 mM), then stored at 0 °C for 4 weeks. Fruit treated with 1 and 2 mM NaHS in both cultivars had the significantly higher firmness and lower respiration rate than the control fruit. However, no treatment retarded the losses in soluble solids content (SSC) and titratable acidity (TA). After 4 weeks of storage, H2S suppressed stem browning, decay, and surface pitting, but it did not affect the weight loss. The greater positive effect of H2S for reducing surface pitting development was due to the lower yields of water-soluble polysaccharides (WSP) and CDTA-soluble polysaccharides (CSP) and the inhibited activities of polygalacturonase (PG), pectate lyase (PL), and β-D-galactosidase (β-GAL). Overall, results demonstrated that H2S applied as postharvest vapor is an effective tool to control surface pitting development in sweet cherry with protected cell wall structure and reduction of cell wall metabolism.
{"title":"Effect of hydrogen sulfide on surface pitting and related with cell wall metabolism in sweet cherry during cold storage","authors":"Yu Dong, H. Zhi","doi":"10.5073/JABFQ.2018.091.015","DOIUrl":"https://doi.org/10.5073/JABFQ.2018.091.015","url":null,"abstract":"This study was to determine whether postharvest H2S fumigation effects surface pitting development of ‘Lapins’ and ‘Regina’ cherries after cold storage, and if so, how H2S takes participate in minimizing pitting injury and its relation to cell wall metabolism. Fruit were exposed to hydrogen sulfide (H2S) gas released from sodium hydrosulfide (NaHS at rates of 0.5, 1, 2, or 3 mM), then stored at 0 °C for 4 weeks. Fruit treated with 1 and 2 mM NaHS in both cultivars had the significantly higher firmness and lower respiration rate than the control fruit. However, no treatment retarded the losses in soluble solids content (SSC) and titratable acidity (TA). After 4 weeks of storage, H2S suppressed stem browning, decay, and surface pitting, but it did not affect the weight loss. The greater positive effect of H2S for reducing surface pitting development was due to the lower yields of water-soluble polysaccharides (WSP) and CDTA-soluble polysaccharides (CSP) and the inhibited activities of polygalacturonase (PG), pectate lyase (PL), and β-D-galactosidase (β-GAL). Overall, results demonstrated that H2S applied as postharvest vapor is an effective tool to control surface pitting development in sweet cherry with protected cell wall structure and reduction of cell wall metabolism.","PeriodicalId":56276,"journal":{"name":"Journal of Applied Botany and Food Quality-Angewandte Botanik","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2018-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45450480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-02DOI: 10.5073/JABFQ.2018.091.013
A. L. Hykkerud, E. Uleberg, E. Hansen, Marieke Vervoort, J. Mølmann, I. Martinussen
Cloudberry (Rubus chamaemorus L.) is a wild perennial shrub growing on peatland with a circumpolar distribution. The combined berries have a high polyphenol content comprised primarily of ellagitannins. A few commercial cultivars are available, and pre-breeding trials on clonal material from different geographical origins are in progress. The objective of this study was to investigate how the content of polyphenols of four different cloudberry cultivars were affected by harvesting time and climatic variations during a 3-year-period. Plants were grown outside in plots and berries were harvested when mature. Berries were analyzed for total polyphenols and total anthocyanins by spectrophotometer. Total ellagic acid was identified and quantified using HPLC-MS after hydrolysis of the extracts. Results showed that all measured parameters; total anthocyanins, total polyphenols and ellagic acid are strongly influenced by the genetic background. Although low anthocyanin contents were present in all genotypes, they were highly affected by climatic conditions, being highest at low temperatures. However, the content of ellagic acid was less affected by environmental conditions and showed little response to changing temperatures. In conclusion, ellagitannin content was the most dominating polyphenol group observed in this study and was affected by genetics and is therefore a good breeding criterion for increased health benefit of cloudberry.
{"title":"Seasonal and yearly variation of total polyphenols, total anthocyanins and ellagic acid in different clones of cloudberry (Rubus chamaemorus L.)","authors":"A. L. Hykkerud, E. Uleberg, E. Hansen, Marieke Vervoort, J. Mølmann, I. Martinussen","doi":"10.5073/JABFQ.2018.091.013","DOIUrl":"https://doi.org/10.5073/JABFQ.2018.091.013","url":null,"abstract":"Cloudberry (Rubus chamaemorus L.) is a wild perennial shrub growing on peatland with a circumpolar distribution. The combined berries have a high polyphenol content comprised primarily of ellagitannins. A few commercial cultivars are available, and pre-breeding trials on clonal material from different geographical origins are in progress. The objective of this study was to investigate how the content of polyphenols of four different cloudberry cultivars were affected by harvesting time and climatic variations during a 3-year-period. Plants were grown outside in plots and berries were harvested when mature. Berries were analyzed for total polyphenols and total anthocyanins by spectrophotometer. Total ellagic acid was identified and quantified using HPLC-MS after hydrolysis of the extracts. Results showed that all measured parameters; total anthocyanins, total polyphenols and ellagic acid are strongly influenced by the genetic background. Although low anthocyanin contents were present in all genotypes, they were highly affected by climatic conditions, being highest at low temperatures. However, the content of ellagic acid was less affected by environmental conditions and showed little response to changing temperatures. In conclusion, ellagitannin content was the most dominating polyphenol group observed in this study and was affected by genetics and is therefore a good breeding criterion for increased health benefit of cloudberry.","PeriodicalId":56276,"journal":{"name":"Journal of Applied Botany and Food Quality-Angewandte Botanik","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2018-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42183887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-02DOI: 10.5073/JABFQ.2018.091.012
D. Tom-Dery, Franziska Eller, K. Jensen, C. Reisdorff
Atmospheric carbon dioxide enrichment enhances plant growth and development and may alter the nutritive value of grasses. The objective of this study was to evaluate growth, biomass partitioning and nutritive value of Kyasuwa under combinations of atmospheric CO2 concentrations, watering and fertilization treatments. Plants were grown in two greenhouse chambers; with ambient (aCO2; 400 ppm) and elevated CO2 (eCO2; 950 ppm), two watering and three fertilization regimes. Elevated CO2 reduced stomatal conductance by 40%, root to shoot ratio by 8%, leaf to stem ratio (L:S) by 3%, protein content by 14% and Acid Detergent Lignin (ADL) by 23% with no significant changes in total biomass and C/N ratio however, slight increases in leaf area (2%) and Acid Detergent Fiber (ADF) by 4%. Higher fertilization resulted in increased biomass parameters only in well-watered plants while; a lower C/N ratio was recorded with higher fertilization. The L:S ratio was decreased with fertilization while ADL was increased at higher fertilization in well-watered plants. Interactive effects were recorded for ADF content and shoot height. Future eCO2 will be unfavorable to Kyasuwa growth and biomass production making them less competitive with a reduced nutritive value in drought prone and infertile soils.
{"title":"Effects of elevated carbon dioxide and climate change on biomass and nutritive value of Kyasuwa (Cenchrus pedicellatus Trin.)","authors":"D. Tom-Dery, Franziska Eller, K. Jensen, C. Reisdorff","doi":"10.5073/JABFQ.2018.091.012","DOIUrl":"https://doi.org/10.5073/JABFQ.2018.091.012","url":null,"abstract":"Atmospheric carbon dioxide enrichment enhances plant growth and development and may alter the nutritive value of grasses. The objective of this study was to evaluate growth, biomass partitioning and nutritive value of Kyasuwa under combinations of atmospheric CO2 concentrations, watering and fertilization treatments. Plants were grown in two greenhouse chambers; with ambient (aCO2; 400 ppm) and elevated CO2 (eCO2; 950 ppm), two watering and three fertilization regimes. Elevated CO2 reduced stomatal conductance by 40%, root to shoot ratio by 8%, leaf to stem ratio (L:S) by 3%, protein content by 14% and Acid Detergent Lignin (ADL) by 23% with no significant changes in total biomass and C/N ratio however, slight increases in leaf area (2%) and Acid Detergent Fiber (ADF) by 4%. Higher fertilization resulted in increased biomass parameters only in well-watered plants while; a lower C/N ratio was recorded with higher fertilization. The L:S ratio was decreased with fertilization while ADL was increased at higher fertilization in well-watered plants. Interactive effects were recorded for ADF content and shoot height. Future eCO2 will be unfavorable to Kyasuwa growth and biomass production making them less competitive with a reduced nutritive value in drought prone and infertile soils.","PeriodicalId":56276,"journal":{"name":"Journal of Applied Botany and Food Quality-Angewandte Botanik","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2018-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44624901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-02DOI: 10.5073/JABFQ.2018.091.014
M. Maszewska, A. Florowska, K. Matysiak, K. Marciniak-Łukasiak, E. Dłużewska
Palm oil is characterized by high oxidation stability, high smoke point, low foam making properties, limited penetration into the product, what makes it ideal for processes requiring thermal treatment such as frying. The aim of the study was to investigate the chemical composition and thermooxidative stability of red palm olein, rapeseed oil and their mixtures during deep-frying of French fries. Analysis of fatty acids composition and basic parameters of fresh oils (acid number, peroxide value, polar compounds content, induction time) were performed. During frying, changes in acid number, polar compounds in oils as well as consumers’ acceptance of the fries fried in these oils were investigated. During the 32-hour of frying, the lowest chemical changes occurred in palm olein, what was confirmed by low acid values (0.99 mg KOH/g) and low polar content (14.4%). At the end of the experiment, the oil mixture had the highest polar fraction value of 25.0%. In the opinion of consumers, fries fried in rapeseed oil were “the best”, while French fries fried on palm oil were considered “artificial”, “chemical” and “disgusting”. The reason for this opinion was the addition of β-carotene to this oil. On the other hand β-carotene from palm olein had a great positive effect on the colour of the fries, but at the same time had a negative effect on the taste.
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