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Development of a SYBR Green Real-Time PCR method for the detection of Coffea arabica and C. canephora using chloroplast genes 利用叶绿体基因开发用于检测阿拉伯咖啡和印度咖啡的 SYBR Green Real-Time PCR 方法
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-25 DOI: 10.1007/s10068-024-01636-7
Ju Hee Kim, Cheol Min Kim, Cheol Seong Jang

Coffea arabica (Arabica) and C. canephora (Robusta) are valuable agricultural products traded worldwide. In this study, we designed specific primer pairs for Arabica and Robusta using chloroplast genes to distinguish and quantify the two types of coffee beans. We assessed the specificity, sensitivity, and applicability of the qRT-PCR assay using all the primer pairs. The six designed primer pairs (three for Arabica and three for Robusta) exhibited a correlation coefficient higher than 0.99 and a slope of approximately − 3.21 to − 3.52. The efficiency ranged from 92.09 to 104.79%. The Real-Time quantitative PCR (qPCR) assay had a detection limit of 0.001 ng DNA and a quantitative detection limit of 0.01% (w/w). Additionally, the specificity of the primer pairs was confirmed by analyzing 12 non-target plant species and verifying their practicality using 10 commercials. This study highlights the effectiveness of the SYBR-based qPCR assay in detecting adulteration in commercial coffee products.

阿拉比卡咖啡豆(Arabica)和罗布斯塔咖啡豆(C. canephora)是全球交易的珍贵农产品。在这项研究中,我们利用叶绿体基因为阿拉比卡咖啡豆和罗布斯塔咖啡豆设计了特定的引物对,以区分和量化这两种咖啡豆。我们使用所有引物对评估了 qRT-PCR 检测的特异性、灵敏度和适用性。设计的六对引物(三对用于阿拉比卡咖啡豆,三对用于罗布斯塔咖啡豆)的相关系数高于 0.99,斜率约为 - 3.21 至 - 3.52。效率在 92.09% 到 104.79% 之间。实时定量 PCR(qPCR)测定的检测限为 0.001 ng DNA,定量检测限为 0.01%(重量比)。此外,通过分析 12 种非目标植物物种和使用 10 种商品验证引物对的实用性,证实了引物对的特异性。本研究强调了基于 SYBR 的 qPCR 检测法在检测商业咖啡产品掺假方面的有效性。
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引用次数: 0
Acrylamide formation in air-fryer roasted legumes as affected by legume species and roasting degree: the correlation of acrylamide with asparagine and free sugars 风干烘烤豆类中丙烯酰胺的形成受豆类种类和烘烤程度的影响:丙烯酰胺与天门冬酰胺和游离糖的相关性
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-25 DOI: 10.1007/s10068-024-01633-w
Mun Yhung Jung, Chung Hun Baek, Yongzhe Ma, Hee Won Lee

Acrylamide is a well-recognized hazardous compound with known carcinogenic, genotoxic, neurotoxic, and reproductive toxic effects. This research aimed to investigate how different legume species and roasting durations influence acrylamide formation during air-fryer roasting. The study also examined the relationship between acrylamide formation and the levels of free asparagine and free sugars in different bean species. Asparagine content varies substantially across different bean species. Sucrose was the predominant sugar across all bean species, with smaller amounts of galactose and glucose. Air-fryer-roasted Wandu kong (garden pea) showed the highest acrylamide formation, followed by Ultari kong (kidney bean) and Heoktae (black soybean), in that order. Beans roasted for longer periods in an air fryer contained significantly higher levels of acrylamide. This study revealed a strong positive correlation between acrylamide formation and the level of free asparagine in the beans, highlighting the risks associated with certain legume species and air-fryer roasting durations.

丙烯酰胺是一种公认的有害化合物,具有已知的致癌性、遗传毒性、神经毒性和生殖毒性。这项研究旨在调查在空气炸锅烘烤过程中,不同豆类品种和烘烤时间如何影响丙烯酰胺的形成。研究还考察了丙烯酰胺的形成与不同豆类品种中游离天冬酰胺和游离糖含量之间的关系。不同豆类品种的天门冬酰胺含量差异很大。蔗糖是所有豆类品种中的主要糖类,半乳糖和葡萄糖的含量较少。经空气油炸机烘烤的万豆公(园豌豆)丙烯酰胺含量最高,其次依次是Ultari kong(芸豆)和Heoktae(黑大豆)。在空气炸锅中长时间烘烤的豆类丙烯酰胺含量明显更高。这项研究表明,丙烯酰胺的形成与豆类中游离天门冬酰胺的含量之间存在着很强的正相关性,突出表明了某些豆类品种和空气炸锅烘烤时间所带来的风险。
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引用次数: 0
Extraction and characterization of mung bean proteins using different alkaline solutions 使用不同碱性溶液提取绿豆蛋白质并确定其特征
IF 2.4 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-24 DOI: 10.1007/s10068-024-01624-x
Jae Won Kim, Hyun-Seok Kim

This study investigated the effects of alkaline solutions on the production and characteristics of mung bean proteins (MBPs). MBPs were prepared using alkaline solutions of NaOH, NaHCO3, and Na2CO3 and designated MPN, MPH, and MPC, respectively. The yield, protein recovery, and crude protein content of MBP were not significantly different at different alkali concentrations (0.01–0.1%). Although there was no significant difference in MBP yield between alkali types, protein recovery and crude protein content increased in the following order: MPN > MPC > MPH. The essential and branched-chain amino acid contents, molecular weight distribution, and ζ-potential did not differ between MBPs. Regarding MBP pH-dependent solubility, MPN solubility was lower at pH 6–8 than that of MPH and MPC. This pattern was commonly observed for other physical properties. Overall, MBP was prepared using NaHCO3, and Na2CO3 and its functional properties were better when Na2CO3 was used than when NaOH was used.

本研究调查了碱性溶液对绿豆蛋白(MBPs)的生产和特性的影响。使用 NaOH、NaHCO3 和 Na2CO3 碱性溶液制备 MBP,并分别命名为 MPN、MPH 和 MPC。在不同的碱浓度(0.01%-0.1%)下,MBP 的产量、蛋白质回收率和粗蛋白含量没有显著差异。虽然不同碱类型的 MBP 产量无明显差异,但蛋白质回收率和粗蛋白含量依次增加:MPN;MPC;MPH。不同 MBP 的必需氨基酸和支链氨基酸含量、分子量分布和 ζ 电位没有差异。关于 MBP 随 pH 值变化的溶解度,MPN 在 pH 值为 6-8 时的溶解度低于 MPH 和 MPC。其他物理性质也普遍存在这种模式。总体而言,使用 NaHCO3 和 Na2CO3 制备 MBP 时,其功能特性优于使用 NaOH 时。
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引用次数: 0
Research trends of next generation probiotics 新一代益生菌的研究趋势
IF 2.4 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-24 DOI: 10.1007/s10068-024-01626-9
Muhammad Adeel Hasnain, Dae‑Kyung Kang, Gi-Seong Moon

Gut represents one of the largest interfaces for the interaction of host factors and the environmental ones. Gut microbiota, largely dominated by bacterial community, plays a significant role in the health status of the host. The healthy gut microbiota fulfills several vital functions such as energy metabolism, disease protection, and immune modulation. Dysbiosis, characterized by microbial imbalance, can contribute to the development of various disorders, including intestinal, systemic, metabolic, and neurodegenerative conditions. Probiotics offer the potential to address dysbiosis and improve overall health. Advancements in high-throughput sequencing, bioinformatics, and omics have enabled mechanistic studies for the development of bespoke probiotics, referred to as next generation probiotics. These tailor-made probiotics have the potential to ameliorate specific disease conditions and thus fulfill the specific consumer needs. This review discusses recent updates on the most promising next generation probiotics, along with the challenges that must be addressed to translate this concept into reality.

肠道是宿主因素和环境因素相互作用的最大界面之一。肠道微生物群主要由细菌群落主导,对宿主的健康状况起着重要作用。健康的肠道微生物群具有多种重要功能,如能量代谢、疾病保护和免疫调节。以微生物失衡为特征的菌群失调会导致各种疾病的发生,包括肠道疾病、系统性疾病、代谢性疾病和神经退行性疾病。益生菌具有解决菌群失调和改善整体健康的潜力。高通量测序、生物信息学和 Omics 技术的进步为开发定制益生菌(即新一代益生菌)提供了机理研究的机会。这些量身定制的益生菌有可能改善特定的疾病状况,从而满足消费者的特定需求。本综述将讨论最有前景的下一代益生菌的最新进展,以及将这一概念转化为现实所必须应对的挑战。
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引用次数: 0
Analysis of 8-oxooctanoate, 9-oxononanoate, fatty acids, oxidative stability, and iodine value during deep-frying of French fries in edible oils blended with palm oil 用棕榈油调和的食用油油炸炸薯条时的 8-氧代辛酸酯、9-氧代壬酸酯、脂肪酸、氧化稳定性和碘值分析
IF 2.4 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-24 DOI: 10.1007/s10068-024-01615-y
Yejin Choi, Dohyoung Lee, Jonggab Yim, Kwang-Geun Lee

Four blended oils including palm + corn oil (PC), palm + grapeseed oil (PG), corn + grapeseed oil (CG), and palm + corn + grapeseed oil (PCG) were used as frying media. The 8-oxo and 9-oxo content, fatty acid composition, oxidative stability index, and iodine value of samples (n = 42) were analyzed. As the frying cycles increased, the content of 8-oxo and 9-oxo also increased significantly (p < 0.05) and showed the highest content of 965.56 ± 0.706 μg/g in 100 frying cycles of PCG. After 20–40 frying cycles in CG, the amount of palmitic, stearic, oleic and linoleic acid increased up to 15.5% (p < 0.05). The oxidative stability of palm oil was the highest at 16.76–18.33 h (p < 0.05). The iodine value of grapeseed oil was the highest at 144.96 ± 2.569–153.64 ± 2.233 g iodine/100 g oil (p < 0.05). Among the examined oils, PC was the most suitable alternative to palm oil.

采用四种混合油作为油炸介质,包括棕榈油+玉米油(PC)、棕榈油+葡萄籽油(PG)、玉米油+葡萄籽油(CG)和棕榈油+玉米油+葡萄籽油(PCG)。对样品(n = 42)的 8-氧代和 9-氧代含量、脂肪酸组成、氧化稳定性指数和碘值进行了分析。随着油炸次数的增加,8-氧代和 9-氧代的含量也显著增加(p < 0.05),在 PCG 100 次油炸中,8-氧代和 9-氧代的含量最高,为 965.56 ± 0.706 μg/g。在 CG 中经过 20-40 个油炸循环后,棕榈酸、硬脂酸、油酸和亚油酸的含量增加了 15.5% (p < 0.05)。棕榈油的氧化稳定性在 16.76-18.33 h 时最高(p < 0.05)。葡萄籽油的碘值最高,为 144.96 ± 2.569-153.64 ± 2.233 克碘/100 克油(p < 0.05)。在所研究的油类中,PC 是最适合替代棕榈油的油类。
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引用次数: 0
Enhancement of sausages shelf life using natural antimicrobials and propolis extract 利用天然抗菌剂和蜂胶提取物提高香肠的保质期
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-24 DOI: 10.1007/s10068-024-01632-x
Milad Yaghoubi, Kazem Alirezalu, Sahar Nottagh, Seyed Hadi Naghizadeh, Seyed Amir Seyed Moslemi, Majid Olyayee, Mahnaz Manafi Dizajyekan, Bahram Fathi-Achachlouei

The present work assessed the impacts of nisin (200 mg/kg) and nisin-nanoparticles (NNPs; 200 mg/kg) in combination with propolis ethanolic extract (PEE; 1% and 3%) on quality and stability of sausage during refrigerated period. The treated meat batters were mechanically stuffed into polyamide casings, packaged in vacuum conditions and analyzed at days 1, 15, 30 and 45. Sausages treated with combined NNPs and PEE displayed higher total phenolic content (2.14–5.41 mg GAE/g DM), pH values (5.27–5.80) and sensory scores (3.70–3.93) with low hardness (34.9–37.7 kg m/s2) and TBARS values (1.41–2.85 mg MDA/kg) throughout keeping period. At day 45, treated samples with 200 mg/kg NNPs + 3% PEE indicated 0.28 Log CFU/g reduction in TVC, 0.16 CFU/g in Clostridium perfringens, 0.23 in E. coli, 0.65 in Staphylococcus aureus, and 0.37 CFU/g in mold and yeast count compared with control (120 ppm nitrite). Hence, this work aims to assess the performance of nisin compounds + PEE in the quality improvement of frankfurter-type sausage kept for 45 days (4 °C) to produce novel and practical nitrite substitutes.

本研究评估了尼生素(200 毫克/千克)和尼生素纳米粒子(NNPs;200 毫克/千克)与蜂胶乙醇提取物(PEE;1% 和 3%)在冷藏期间对香肠质量和稳定性的影响。经处理的肉糊被机械塞入聚酰胺肠衣,在真空条件下包装,并在第 1、15、30 和 45 天进行分析。在整个保存期间,经 NNPs 和 PEE 混合处理的香肠显示出较高的总酚含量(2.14-5.41 毫克 GAE/g DM)、pH 值(5.27-5.80)和感官评分(3.70-3.93),且硬度(34.9-37.7 千克 m/s2)和 TBARS 值(1.41-2.85 毫克 MDA/千克)较低。在第 45 天,与对照组(120 ppm 亚硝酸盐)相比,使用 200 mg/kg NNPs + 3% PEE 处理的样品 TVC 降低了 0.28 Log CFU/g,产气荚膜梭菌降低了 0.16 CFU/g,大肠杆菌降低了 0.23 CFU/g,金黄色葡萄球菌降低了 0.65 CFU/g,霉菌和酵母菌数量降低了 0.37 CFU/g。因此,这项工作旨在评估尼生素化合物 + PEE 在改善保存 45 天(4 ℃)的法兰克福香肠质量方面的性能,以生产新型实用的亚硝酸盐替代品。
{"title":"Enhancement of sausages shelf life using natural antimicrobials and propolis extract","authors":"Milad Yaghoubi, Kazem Alirezalu, Sahar Nottagh, Seyed Hadi Naghizadeh, Seyed Amir Seyed Moslemi, Majid Olyayee, Mahnaz Manafi Dizajyekan, Bahram Fathi-Achachlouei","doi":"10.1007/s10068-024-01632-x","DOIUrl":"https://doi.org/10.1007/s10068-024-01632-x","url":null,"abstract":"<p>The present work assessed the impacts of nisin (200 mg/kg) and nisin-nanoparticles (NNPs; 200 mg/kg) in combination with propolis ethanolic extract (PEE; 1% and 3%) on quality and stability of sausage during refrigerated period. The treated meat batters were mechanically stuffed into polyamide casings, packaged in vacuum conditions and analyzed at days 1, 15, 30 and 45. Sausages treated with combined NNPs and PEE displayed higher total phenolic content (2.14–5.41 mg GAE/g DM), pH values (5.27–5.80) and sensory scores (3.70–3.93) with low hardness (34.9–37.7 kg m/s<sup>2</sup>) and TBARS values (1.41–2.85 mg MDA/kg) throughout keeping period. At day 45, treated samples with 200 mg/kg NNPs + 3% PEE indicated 0.28 Log CFU/g reduction in TVC, 0.16 CFU/g in <i>Clostridium perfringens,</i> 0.23 in <i>E. coli</i>, 0.65 i<i>n Staphylococcus aureus</i>, and 0.37 CFU/g in mold and yeast count compared with control (120 ppm nitrite). Hence, this work aims to assess the performance of nisin compounds + PEE in the quality improvement of frankfurter-type sausage kept for 45 days (4 °C) to produce novel and practical nitrite substitutes.</p>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"22 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141512332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Postbiotic nanoparticles (postbiotics-NPs): a novel strategy for providing probiotics’ health advantages through food consumption 益生菌后纳米颗粒(postbiotics-NPs):通过食用提供益生菌健康优势的新策略
IF 2.4 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-23 DOI: 10.1007/s10068-024-01629-6
Sarhan Mohammed, Ahmet Hilmi Çon

In recent years, the term “postbiotics” has become increasingly popular in food microbiology, food science, and commercial products. This importance has been raised due to the safety issues associated with live bacterial cells. Postbiotics are defined as bioactive substances of probiotics. It confers unique health-promoting functions with its chemical structure, safe profile, and long shelf life. Meanwhile, postbiotics nanoparticles (postbiotics-NPs) can be considered novel postbiotic delivery systems to deliver bioactive components with health benefits and therapeutic aims, promote the shelf-life of food products, and develop novel functional foods. The present scientific literature shows that nanotechnology approaches are not yet mature enough to be used in postbiotic delivery systems. For all of that, the potential applications of postbiotics-NPs in the food industry and biomedical fields will be a new trend in the future.

近年来,"后益生菌 "一词在食品微生物学、食品科学和商业产品中越来越受欢迎。这一重要性的提高是由于与活细菌细胞相关的安全问题。后益生菌被定义为益生菌的生物活性物质。它以其化学结构、安全特性和较长的保质期赋予了独特的健康促进功能。同时,纳米益生菌后颗粒(postbiotics-NPs)可被视为新型益生菌后传递系统,可传递具有健康益处和治疗目的的生物活性成分,延长食品的保质期,开发新型功能食品。目前的科学文献表明,纳米技术方法尚未成熟到可用于益生菌后输送系统。尽管如此,后生物素-NPs 在食品工业和生物医学领域的潜在应用将是未来的一个新趋势。
{"title":"Postbiotic nanoparticles (postbiotics-NPs): a novel strategy for providing probiotics’ health advantages through food consumption","authors":"Sarhan Mohammed,&nbsp;Ahmet Hilmi Çon","doi":"10.1007/s10068-024-01629-6","DOIUrl":"10.1007/s10068-024-01629-6","url":null,"abstract":"<div><p>In recent years, the term “postbiotics” has become increasingly popular in food microbiology, food science, and commercial products. This importance has been raised due to the safety issues associated with live bacterial cells. Postbiotics are defined as bioactive substances of probiotics. It confers unique health-promoting functions with its chemical structure, safe profile, and long shelf life. Meanwhile, postbiotics nanoparticles (postbiotics-NPs) can be considered novel postbiotic delivery systems to deliver bioactive components with health benefits and therapeutic aims, promote the shelf-life of food products, and develop novel functional foods. The present scientific literature shows that nanotechnology approaches are not yet mature enough to be used in postbiotic delivery systems. For all of that, the potential applications of postbiotics-NPs in the food industry and biomedical fields will be a new trend in the future.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"33 12","pages":"2729 - 2736"},"PeriodicalIF":2.4,"publicationDate":"2024-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141512331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of the region-specific characteristics of traditional Korean doenjang produced in Gyeonggi-do 京畿道生产的韩国传统豆瓣酱的地区特点分析
IF 2.4 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-21 DOI: 10.1007/s10068-024-01590-4
Yujin Lee, In-Seo Hwang, Mina K. Kim

Doenjang is traditional soybean fermented food in Korea, and traditional doenjang on the market has different qualities like aroma, taste. This study is a preliminary study to investigate the quality characteristics of doenjang produced in each region of Korea. The objective of this study was to analyze the region-specific characteristics of traditional Korean doenjang produced in Gyeonggi-do. Physiochemical characteristics including pH, moisture content, soluble solid content, salinity, color, acid value, titratable acidity, NH2-N, total/reducing sugar, and alcohol contents, and enzymatic activities such as acidic/neutral protease and α/β-amylase activities, were analyzed. Doenjang produced in Gyeonggi-do was classified into two groups (GDG-A and GDG-B), and the distinction between two groups were the aging period: doenjang samples in GDG-A groups were aged over 3 yrs, while samples in GDG-B groups were aged less than 3 yrs. The results of this study provided the physicochemical characteristics and enzymatic activities of traditional doenjang produced in Gyeonggi-do.

豆酱是韩国传统的大豆发酵食品,市场上的传统豆酱具有不同的品质,如香味、口感等。本研究是一项初步研究,旨在调查韩国各地区生产的豆酱的质量特点。本研究的目的是分析京畿道生产的韩国传统豆酱的地区特性。研究分析了 pH 值、水分含量、可溶性固体含量、盐度、颜色、酸值、可滴定酸度、NH2-N、总糖/还原糖和酒精含量等理化特性,以及酸性/中性蛋白酶和 α/β 淀粉酶活性等酶活性。京畿道生产的豆酱分为两组(GDG-A 和 GDG-B),两组之间的区别在于陈酿时间:GDG-A 组的豆酱样品陈酿时间超过 3 年,而 GDG-B 组的样品陈酿时间不足 3 年。本研究结果提供了京畿道传统豆酱的理化特性和酶活性。
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引用次数: 0
Pichia kudriavzevii UNJCC Y-137 and Candida tropicalis UNJCC Y-140 isolated from Durio kutejensis as potential probiotic agents 从 Durio kutejensis 分离出的 Pichia kudriavzevii UNJCC Y-137 和 Candida tropicalis UNJCC Y-140 是潜在的益生菌剂
IF 2.4 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-21 DOI: 10.1007/s10068-024-01609-w
Dalia Sukmawati, Adisyahputra Adisyahputra, Laith Khalil Tawfeeq Al-Ani, Shabrina Nida Al Husna, Zakiah Nur Afifah, Catur Sriherwanto, Surono Surono, R. Haryo Bimo Setiarto, Muktiningsih Nurjayadi, Roshanida A. Rahman

Durio kutejensis, commonly known as Lai durian, has a unique characteristics of a creamy texture and a combination of sweet and bitter tastes. This study aimed to isolate and screen yeast from fruits as a potential probiotic agent. The tests consisted of tolerance to bile salt and gastric acid at pH 2, antibacterial activity against Listeria monocytogenes and Salmonella enteriditis, and hemolytic activity on blood agar medium. The results showed that 40 yeasts isolated from Lai durian fruit and 34 of these isolates grew on YMA medium. The two isolates showed high significance in the probiotic tests. These two isolates were able to grow on bile salt up to a concentration of 2% and gastric acid for up to 6 h, with survival rates of 99.06% and 100%, respectively. Two isolates were identified as Pichia kudriavzevii UNJCC Y-137 and Candida tropicalis UN-JCC Y-140 Therefore, these two yeast isolates can be used as potential probiotic agents.

俗称 "黎榴 "的榴莲(Durio kutejensis)具有独特的奶油质地和甜苦结合的口味。本研究旨在从水果中分离和筛选酵母,作为潜在的益生菌剂。测试包括对胆盐和 pH 值为 2 的胃酸的耐受性、对李斯特菌和肠炎沙门氏菌的抗菌活性,以及在血液琼脂培养基上的溶血活性。结果表明,从荔枝果中分离出 40 种酵母菌,其中 34 种在 YMA 培养基上生长。其中两个分离物在益生菌测试中表现出了很高的重要性。这两种分离物能在浓度达 2% 的胆盐和胃酸中生长长达 6 小时,存活率分别为 99.06% 和 100%。因此,这两种酵母分离物可用作潜在的益生菌制剂。
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引用次数: 0
Characterization of potato starch-high amylose rice starch blend as a substitute of acetylated potato starch in long-life noodle 马铃薯淀粉-高淀粉水稻淀粉混合物作为乙酰化马铃薯淀粉替代品在长寿面中的表征
IF 2.4 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-20 DOI: 10.1007/s10068-024-01628-7
Min Ji Cho, Hyun-Seok Kim

This study investigated the suitability of a potato starch (NP)-Dodamssal rice starch (DD) mixture to replace acetylated potato starch (AP) in long-life noodles. Wheat flour (WF) was replaced with AP and NP in 20% of WF, and NP was replaced with DD in 10–50% of NP. The swelling power of the WF-AP mixture was similar to that of all the WF-NP-DD mixtures. The melting enthalpies of the WF-NP-DD mixtures were slightly higher than those of the WF-AP mixtures. The pasting viscosity decreased with increasing DD content of the mixtures. The G′ of all the WF-NP-DD mixtures was higher than that of the WF-AP mixture over the temperature profile, and similar G′ patterns over time were observed. The tensile strengths of noodles by the WF-NP-DD mixtures were similar to those obtained using the WF and WF-AP mixture. Overall, NP-DD mixtures have the potential to replace AP when mixed with WF.

本研究调查了马铃薯淀粉(NP)-多达糙米淀粉(DD)混合物在长寿面中替代乙酰化马铃薯淀粉(AP)的适用性。在 20% 的小麦粉中添加 AP 和 NP,在 10-50% 的 NP 中添加 DD。WF-AP 混合物的膨胀力与所有 WF-NP-DD 混合物相似。WF-NP-DD 混合物的熔化焓略高于 WF-AP 混合物。糊化粘度随着混合物中 DD 含量的增加而降低。在温度曲线上,所有 WF-NP-DD 混合物的 G′都比 WF-AP 混合物的 G′高,而且在不同时间内也观察到类似的 G′变化规律。用 WF-NP-DD 混合物制成的面条的拉伸强度与用 WF 和 WF-AP 混合物制成的面条的拉伸强度相似。总之,NP-DD 混合物与 WF 混合后有可能取代 AP。
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引用次数: 0
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Food Science and Biotechnology
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