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Influence of shifting thermal regimes on tomato fruit borer, Helicoverpa armigera (Hubner) in the Eastern Himalaya: implications for pest management strategies. 东喜马拉雅地区热量变化对番茄果实螟(Helicoverpa armigera (Hubner))的影响:对害虫管理策略的启示。
IF 3 3区 地球科学 Q2 BIOPHYSICS Pub Date : 2024-08-13 DOI: 10.1007/s00484-024-02741-2
Sandip Patra, Debasish Chakraborty, V K Verma, Rachna Pande, Rumki H Ch Sangma, Mahasweta Chakraborty, Jayanta Layek, S Hazarika

Climate change, particularly temperature fluctuations, profoundly impacts pest populations. This study focuses on the tomato, a crucial commercial crop in the Eastern Himalayan Region of India. The study examined the impact of varying thermal regimes on tomato fruit borers. Field experiments were conducted at three locations, with altitudes ranging from < 500 to > 1500 m. At lower altitudes, fruit borer incidence commenced earlier (5th - 18th March) and peaked higher (1.47 ± 0.34 to 1.73 ± 0.37 larvae/plant), causing more damage (26-29%) as compared to the highest location (~ 9%). The generalized linear mixed model (GLMM) analysis indicated that maximum temperature had significant positive impacts on the H. armigera incidence and fruit damage. Climatic datasets indicate an increase in the temperature of the region during the tomato growing season, thereby increasing the risk of fruit borer impact. As an adaptation option, we evaluated eight different tomato varieties/genotypes and studied biochemical parameters to understand their tolerance. Results showed a strong positive association of fruit borer incidence with total soluble solids whereas negative association with acidity. Cherry tomato (7.62%) and MT-2 (10.04%) had relatively lower fruit damage; MT-3 (50.92 t/ha) and MT-2 (50.57 t/ha) consistently yielded the highest across all locations. Hence, the selection of appropriate genotypes and the development of varieties with suitable characteristics hold the key to fruit borer management. This insight is crucial for developing effective pest management strategies and ensuring sustainable agricultural practices in the region.

气候变化,尤其是温度波动,对害虫种群产生了深远影响。本研究以番茄为重点,番茄是印度东喜马拉雅地区的重要经济作物。研究考察了不同热量制度对番茄果实蛀虫的影响。在海拔 1500 米以上的三个地点进行了田间试验。与海拔最高的地点(约 9%)相比,在海拔较低的地点,果实蛀心虫的发生率开始较早(3 月 5 日至 18 日),峰值较高(1.47 ± 0.34 至 1.73 ± 0.37 幼虫/株),造成的危害更大(26-29%)。广义线性混合模型(GLMM)分析表明,最高气温对 H. armigera 发生率和果实受害率有显著的正向影响。气候数据集表明,番茄生长季节该地区气温升高,从而增加了果实蛀虫的影响风险。作为一种适应方案,我们评估了八个不同的番茄品种/基因型,并研究了生化参数,以了解它们的耐受性。结果显示,果实蛀虫发生率与总可溶性固形物呈强正相关,而与酸度呈负相关。樱桃番茄(7.62%)和 MT-2(10.04%)的果实受害率相对较低;MT-3(50.92 吨/公顷)和 MT-2(50.57 吨/公顷)在所有地点的产量一直最高。因此,选择合适的基因型和培育具有合适特性的品种是管理果实蛀虫的关键。这一见解对于制定有效的害虫管理战略和确保该地区的可持续农业实践至关重要。
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引用次数: 0
Artificial neural networks as a tool for seasonal forecast of attack intensity of Spodoptera spp. in Bt soybean. 人工神经网络作为一种工具,用于 Bt 大豆中 Spodoptera spp.攻击强度的季节性预测。
IF 3 3区 地球科学 Q2 BIOPHYSICS Pub Date : 2024-08-13 DOI: 10.1007/s00484-024-02747-w
Luciano Cardoso de França, Poliana Silvestre Pereira, Renato Almeida Sarmento, Alice Barbutti Barreto, Jhersyka da Silva Paes, Daiane das Graças do Carmo, Hugo Daniel Dias de Souza, Marcelo Coutinho Picanço

Soybean (Glycine max) is the world's most cultivated legume; currently, most of its varieties are Bt. Spodoptera spp. (Lepidoptera: Noctuidae) are important pests of soybean. An artificial neural network (ANN) is an artificial intelligence tool that can be used in the study of spatiotemporal dynamics of pest populations. Thus, this work aims to determine ANN to identify population regulation factors of Spodoptera spp. and predict its density in Bt soybean. For two years, the density of Spodoptera spp. caterpillars, predators, and parasitoids, climate data, and plant age was evaluated in commercial soybean fields. The selected ANN was the one with the weather data from 25 days before the pest's density evaluation. ANN forecasting and pest densities in soybean fields presented a correlation of 0.863. It was found that higher densities of the pest occurred in dry seasons, with less wind, higher atmospheric pressure and with increasing plant age. Pest density increased with the increase in temperature until this curve reached its maximum value. ANN forecasting and pest densities in soybean fields in different years, seasons, and stages of plant development were similar. Therefore, this ANN is promising to be implemented into integrated pest management programs in soybean fields.

大豆(Glycine max)是世界上栽培面积最大的豆科植物;目前,它的大多数品种都是 Bt 大豆。Spodoptera spp.(鳞翅目:夜蛾科)是大豆的重要害虫。人工神经网络(ANN)是一种人工智能工具,可用于研究害虫种群的时空动态。因此,本研究旨在利用人工神经网络确定 Spodoptera spp.的种群调控因素,并预测其在 Bt 大豆中的密度。两年来,对商业大豆田中 Spodoptera spp.毛虫、天敌和寄生虫的密度、气候数据和植株年龄进行了评估。所选的 ANN 是害虫密度评估前 25 天的天气数据。ANN 预测与大豆田害虫密度的相关性为 0.863。研究发现,在风力较小、气压较高和植株年龄增加的干旱季节,害虫密度较高。害虫密度随着温度的升高而增加,直到该曲线达到最大值。ANN 预测结果与不同年份、季节和植物生长阶段大豆田中的害虫密度相似。因此,在大豆田害虫综合治理计划中实施这种方差网络是很有前途的。
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引用次数: 0
Introducing PLEMS: the application of a low-cost, portable monitoring system in environmental walks. 介绍 PLEMS:低成本便携式监测系统在环境行走中的应用。
IF 3 3区 地球科学 Q2 BIOPHYSICS Pub Date : 2024-08-08 DOI: 10.1007/s00484-024-02754-x
Eduardo Krüger, Walter Ihlenfeld, Ivan Callejas, Solange Leder

The application of innovative systems using low-cost microcontrollers in human biometeorology studies is a promising alternative to conventional monitoring devices, which are usually cost-intensive and provide measurements at specific points, as in stationary meteorological stations. A Portable Low-cost Environmental Monitoring System (PLEMS) aimed at the pedestrian scale is introduced. The backpack-type equipment consists of a microcontroller with attached sensors that assess environmental conditions in a broad sense, integrating measurements of air quality, lighting and noise levels alongside variables typically measured at meteorological stations. The application of the system took place in altogether 12 environmental walks carried out with questionnaire-surveys with concurrent environmental monitoring with the PLEMS in Curitiba, Brazil, a subtropical location characterized by a Cfb climate type. Results allowed us to test the equipment and method of data gathering within a limited period (approximately 50 min) and for a short walking circuit of 800 m. The equipment was successfully able to capture even slightest differences in environmental conditions among points of interest, whereas subjective responses (n= 3843 responses to a total of 11 questions) showed consistency with measured data. From a multi-domain perspective, relevant insights could be obtained for the measured variables.

在人体生物气象学研究中应用使用低成本微控制器的创新系统,是传统监测设备的一个很有前途的替代方案,因为传统监测设备通常成本高昂,而且是在特定点进行测量,如固定气象站。本文介绍了一种针对行人的便携式低成本环境监测系统(PLEMS)。这种背包式设备由一个微控制器和附带的传感器组成,可从广义上评估环境条件,将空气质量、照明和噪音水平的测量与气象站通常测量的变量结合起来。该系统在巴西库里提巴(Curitiba)共进行了 12 次环境徒步旅行,通过问卷调查和 PLEMS 同时进行环境监测,库里提巴是一个亚热带地区,属于 Cfb 气候类型。结果使我们能够在有限的时间内(约 50 分钟)对设备和数据收集方法进行测试,并进行了 800 米的短途步行。设备成功地捕捉到了兴趣点之间最细微的环境条件差异,而主观回答(共 11 个问题,3843 个回答)显示与测量数据一致。从多领域的角度来看,测量变量可以获得相关的见解。
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引用次数: 0
Advancing mastitis assessment in dairy bovines via short milking tube thermography: A seasonal perspective. 通过短挤奶管热成像技术推进奶牛乳腺炎评估:季节性视角。
IF 3 3区 地球科学 Q2 BIOPHYSICS Pub Date : 2024-08-07 DOI: 10.1007/s00484-024-02743-0
S L Gayathri, M Bhakat, T K Mohanty

In India, where dairy production leads globally, infrared thermography (IRT) and short milking tube thermography specifically are vital for managing mastitis. Therefore, the present study focuses on thermal imaging of the udder and short milking tube (SMT) of the milking machine during the peak milking process of Sahiwal cows and Murrah buffaloes during winter, summer, rainy and autumn seasons to identify sub-clinical (SCM) and clinical mastitis (CM) cases using the Darvi DTL007 camera. The udder health was assessed using the California Mastitis Test, Somatic Cell Count (SCC) and IRT throughout the year. Log10SCC and thermogram analysis revealed a difference (p < 0.01) between healthy, SCM, and CM cases during different seasons in both breeds. Further results showed an increase (p < 0.01) in SMT thermograms of SCM and CM cases compared to healthy quarters in Sahiwal cows during winter, summer, rainy, and autumn were 4.26 and 7.51, 2.37 and 4.47, 2.20 and 3.64, 2.90 and 4.94 ºC, respectively and for Murrah buffaloes were 3.56 and 5.55, 2.70 and 3.81, 1.72 and 3.10, 3.14 and 4.42ºC, respectively. The highest degree of increase in milking udder skin surface temperature and SMT of SCM and CM cases compared to healthy quarters was observed during the winter and the least during the rainy season. Thus, regardless of the seasons examined in this study, SMT thermograms effectively assessed SCM and CM.

在奶制品产量居全球首位的印度,红外热成像(IRT)和短挤奶管热成像对乳腺炎的管理至关重要。因此,本研究使用 Darvi DTL007 摄像机,重点对萨希瓦尔牛(Sahiwal)和缪拉水牛(Murrah buffaloes)在冬季、夏季、雨季和秋季高峰挤奶过程中的乳房和挤奶机短挤奶管(SMT)进行热成像,以识别亚临床(SCM)和临床乳腺炎(CM)病例。全年使用加利福尼亚乳腺炎测试、体细胞计数(SCC)和 IRT 评估乳房健康状况。对数10SCC和热图分析表明(p
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引用次数: 0
Maternal exposure to ambient temperature and birth defects in Brazil: a nationwide case-control study of over 11 million newborns. 巴西孕产妇暴露于环境温度与出生缺陷:一项针对 1100 多万新生儿的全国性病例对照研究。
IF 3 3区 地球科学 Q2 BIOPHYSICS Pub Date : 2024-08-06 DOI: 10.1007/s00484-024-02746-x
Weeberb J Requia, Stefania Papatheodorou

Maternal exposure to extreme ambient temperature during pregnancy has been proposed as a potential risk factor for birth defects. Comprehensive investigations on this association remain limited, particularly in low- and middle-income countries. This study aims to examine the association between ambient temperature exposure during pregnancy and the risk of birth defects in Brazil, contributing to the broader understanding of environmental influences on birth outcomes. Using a large dataset of over 11 million live birth records, we analyzed 12 categories of birth defects, encompassing a time frame from 2001 to 2018. Ambient temperature data were assigned at the municipality level. For the exposure assessment, we considered two biologically driven pregnancy stages by dividing the gestational period into two specific windows: the first trimester (from week 1 to week 12) and the second trimester (from week 13 to week 28). We employed a two-stage case-control design. In the first stage, we applied a conditional logistic regression model to estimate the odds ratio (OR) for specific birth defects and each of the five Brazilian regions (North, Northeast, Midwest, Southeast, and South). The model was adjusted for potential confounding variables, including PM2.5, relative humidity, and socioeconomic status. Temporal trends were addressed using time-stratified sampling. In the second stage, we used mixed-effects meta-analysis to pool region-specific estimates. Our analysis revealed a significant association between maternal exposure to higher ambient temperatures during the first trimester and an increased risk of specific birth defect categories, including those affecting the genital organs (OR = 1.08, 95% CI: 1.02; 1.14), digestive system (OR = 1.12, 95% CI: 1.06; 1.19); circulatory system (OR = 1.08, 95% CI: 1.01; 1.17); eyes, ears, face, and neck (OR = 1.08, 95% CI: 1.02; 1.15); benign neoplasms tumors (OR = 1.17, 95% CI: 1.03; 1.32), musculoskeletal system (OR = 1.03, 95% CI: 1.01; 1.05); and other congenital anomalies (OR = 1.22, 95% CI: 1.15; 1.29). The associations with respiratory system, nervous system, and chromosomal anomalies were null. These findings have significant implications for public health policies aimed at mitigating the impact of environmental factors on birth outcomes, both in Brazil and globally.

孕产妇在怀孕期间暴露于极端环境温度被认为是导致出生缺陷的潜在风险因素。有关这种关联的全面调查仍然有限,尤其是在中低收入国家。本研究旨在探讨巴西孕妇在怀孕期间暴露于环境温度与出生缺陷风险之间的关系,从而帮助人们更广泛地了解环境对出生结果的影响。我们利用一个包含 1100 多万条活产记录的大型数据集,分析了 12 类出生缺陷,时间跨度为 2001 年至 2018 年。环境温度数据按城市级别分配。对于暴露评估,我们考虑了两个生物学驱动的妊娠阶段,将妊娠期分为两个特定窗口:妊娠前三个月(从第 1 周到第 12 周)和妊娠后三个月(从第 13 周到第 28 周)。我们采用了两阶段病例对照设计。在第一阶段,我们采用条件逻辑回归模型来估算特定出生缺陷与巴西五个地区(北部、东北部、中西部、东南部和南部)的几率比(OR)。该模型对潜在的混杂变量(包括 PM2.5、相对湿度和社会经济状况)进行了调整。使用时间分层抽样法解决了时间趋势问题。在第二阶段,我们使用混合效应荟萃分析法汇集了特定地区的估计值。我们的分析表明,孕产妇在怀孕头三个月暴露于较高的环境温度与特定出生缺陷类别的风险增加之间存在明显关联,包括影响生殖器官(OR = 1.08,95% CI:1.02; 1.14)、消化系统(OR = 1.12,95% CI:1.06; 1.19);循环系统(OR = 1.08,95% CI:1.01; 1.17);眼、耳、面部和颈部(OR = 1.08,95% CI:1.02; 1.15);良性肿瘤(OR = 1.17,95% CI:1.03;1.32)、肌肉骨骼系统(OR = 1.03,95% CI:1.01;1.05)和其他先天性异常(OR = 1.22,95% CI:1.15;1.29)。与呼吸系统、神经系统和染色体异常的相关性为零。这些发现对巴西和全球旨在减轻环境因素对出生结果影响的公共卫生政策具有重要意义。
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引用次数: 0
Integrated analysis of remote sensing with meteorological and health data for allergic rhinitis forecasting in Tianjin. 遥感与气象和健康数据在天津过敏性鼻炎预报中的综合分析。
IF 3 3区 地球科学 Q2 BIOPHYSICS Pub Date : 2024-08-06 DOI: 10.1007/s00484-024-02750-1
Yu-Di Guo, Yuan Wang, Wen-Yan Fan, Gen Li

Long time series of vegetation monitoring can be carried out by remote sensing data, the level of urban greening is objectively described, and the spatial characteristics of plant pollen are indirectly understood. Pollen is the main allergen in patients with seasonal allergic rhinitis. Meteorological factors affect the release and diffusion of pollen. Therefore, studying of the complex relationship between meteorological factors and allergic rhinitis is essential for effective prevention and treatment of the disease. In this study, we leverage remote sensing data for a comprehensive decade-long analysis of urban greening in Tianjin, which exhibits an annual increase in vegetative cover of 0.51 per annum, focusing on its impact on allergic rhinitis through changes in pollen distribution. Utilizing high-resolution imagery, we quantify changes in urban Fractional Vegetation Coverage (FVC) and its correlation with pollen types and allergic rhinitis cases. Our analysis reveals a significant correlation between FVC trends and pollen concentrations, with a surprising value of 0.71, highlighting the influence of urban greenery on allergenic pollen levels. We establish a robust connection between the seasonal patterns of pollen outbreaks and allergic rhinitis consultations, with a noticeable increase in consultations during high pollen seasons. our findings indicate a higher allergenic potential of herbaceous compared to woody vegetation. This nuanced understanding underscores the importance of pollen sensitivity, alongside concentration, in driving allergic rhinitis incidents. Utilizing a Generalized Linear Model, significant features influencing the number of visits for allergic rhinitis (P < 0.05) were identified. Both GLM and LSTM models were employed to forecast the visitation volumes for rhinitis during the spring and summer-autumn of 2022. Upon validation, it was found that the R² values between the simulated and actual values for both GLM and LSTM models surpassed the 95% confidence threshold. Moreover, the R² values for the summer-autumn seasons (GLM: 0.56, LSTM: 0.72) were higher than those for spring (GLM: 0.22, LSTM: 0.47). Comparing the errors between the simulated and actual values of GLM and LSTM models, LSTM exhibited higher simulation precision in both spring and summer-autumn seasons, demonstrating superior simulation performance. Overall, our study pioneers the integration of remote sensing with meteorological and health data for allergic rhinitis forecasting. This integrative approach provides valuable insights for public health planning, particularly in urban settings, and lays the groundwork for advanced, location-specific allergenic pollen forecasting and mitigation strategies.

通过遥感数据可以对植被进行长时间序列监测,客观描述城市绿化水平,间接了解植物花粉的空间特征。花粉是季节性过敏性鼻炎患者的主要过敏原。气象因素会影响花粉的释放和扩散。因此,研究气象因素与过敏性鼻炎之间的复杂关系对于有效预防和治疗过敏性鼻炎至关重要。在本研究中,我们利用遥感数据对天津城市绿化进行了长达十年的综合分析,天津的植被覆盖率每年增加 0.51%,重点分析了其通过花粉分布变化对过敏性鼻炎的影响。利用高分辨率图像,我们量化了城市植被覆盖率(FVC)的变化及其与花粉类型和过敏性鼻炎病例的相关性。我们的分析表明,植被覆盖率的变化趋势与花粉浓度之间存在明显的相关性,其相关性值达到了令人惊讶的 0.71,这凸显了城市绿化对过敏性花粉水平的影响。我们在花粉爆发的季节性模式与过敏性鼻炎就诊之间建立了强有力的联系,在花粉高发季节,就诊人数明显增加。这种细致入微的理解强调了花粉敏感性和浓度在过敏性鼻炎发病中的重要性。利用广义线性模型,影响过敏性鼻炎就诊次数的重要特征(P
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引用次数: 0
The influence of air masses on human mortality in the contiguous United States. 气团对美国毗连地区人类死亡率的影响。
IF 3 3区 地球科学 Q2 BIOPHYSICS Pub Date : 2024-08-05 DOI: 10.1007/s00484-024-02745-y
Cameron C Lee, Alindomar Silva, Chibuike Ibebuchi, Scott C Sheridan

Temperature-related mortality is the leading cause of weather-related deaths in the United States. Herein, we explore the effect of air masses (AMs) - a relatively novel and holistic measure of environmental conditions - on human mortality across 61 cities in the United States. Geographic and seasonal differences in the effects of each AM on deseasonalized and detrended anomalous lagged mortality are examined using simple descriptive statistics, one-way analyses of variance, relative risks of excess mortality, and regression-based artificial neural network (ANN) models. Results show that AMs are significantly related to anomalous mortality in most US cities, and in most seasons. Of note, two of the three cool AMs (Cool and Dry-Cool) each show a strong, but delayed mortality response in all seasons, with peak mortality 2 to 4 days after they occur, with the Dry-Cool AM having nearly a 15% increased risk of excess mortality. Humid-Warm (HW) air masses are associated with increases in deaths in all seasons 0 to 1 days after they occur. In most seasons, these near-term mortality increases are offset by reduced mortality for 1-2 weeks afterwards; however, in summer, no such reduction is noted. The Warm and Dry-Warm AMs show slightly longer periods of increased mortality, albeit slightly less intensely as compared with HW, but with a similar lag structure by season. Meanwhile, the most seasonally consistent results are with transitional weather, whereby passing cold fronts are associated with a significant decrease in mortality 1 day after they occur, while warm fronts are associated with significant increases in mortality at that same lag time. Finally, ANN modeling reveals that AM-mortality relationships gleaned from a combined meta-analysis can actually lead to more skillful modeling of these relationships than models trained on some individual cities, especially in the cities where such relationships might be masked due to low average daily mortality.

与气温相关的死亡是美国与天气相关死亡的主要原因。在此,我们探讨了气团(AMs)对美国 61 个城市的人类死亡率的影响,气团是衡量环境条件的一种相对新颖的综合指标。我们使用简单的描述性统计、单向方差分析、超额死亡率相对风险和基于回归的人工神经网络 (ANN) 模型,研究了每种 AM 对去季节化和去趋势化异常滞后死亡率影响的地理和季节差异。结果表明,在美国大多数城市和大多数季节,AMs 与异常死亡率有明显的关系。值得注意的是,三个冷AM中的两个(凉爽和干冷)在所有季节都表现出强烈但延迟的死亡率反应,死亡率峰值出现在它们出现后的2到4天,其中干冷AM的超额死亡率风险增加了近15%。潮湿-温暖(HW)气团出现后 0 到 1 天,所有季节的死亡人数都会增加。在大多数季节,近期死亡率的增加会被随后 1-2 周内死亡率的降低所抵消;但在夏季,则没有这种降低。温暖和干暖型 AM 的死亡率上升期略长,尽管与 HW 相比强度略低,但各季节的滞后结构相似。同时,季节性最一致的结果是过渡性天气,即冷锋过后 1 天,死亡率显著下降,而暖锋过后 1 天,死亡率显著上升。最后,ANN 模型显示,从综合荟萃分析中得出的上午平均气温与死亡率之间的关系,实际上比在某些单个城市训练的模型更能巧妙地模拟这些关系,特别是在那些由于日平均死亡率较低而可能掩盖了这种关系的城市。
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引用次数: 0
Hypoxia related genes modulate in similar fashion in skin fibroblast cells of yak (Bos grunniens) adapted to high altitude and native cows (Bos indicus) adapted to tropical climate during hypoxia stress. 在适应高海拔地区的牦牛(Bos grunniens)和适应热带气候的本地牛(Bos indicus)的皮肤成纤维细胞中,缺氧相关基因在缺氧应激时以类似的方式发生调控。
IF 3 3区 地球科学 Q2 BIOPHYSICS Pub Date : 2024-08-01 Epub Date: 2024-05-30 DOI: 10.1007/s00484-024-02695-5
Manish Tiwari, Monika Sodhi, Manish Sharma, Vishal Sharma, Manishi Mukesh

The present study was conducted to understand transcriptional response of skin fibroblast of yak (Bos grunniens) and cows of Bos indicus origin to hypoxia stress. Six primary fibroblast cell lines derived from three individuals each of Ladakhi yak (Bos grunniens) and Sahiwal cows (Bos indicus) were exposed to low oxygen concentration for a period of 24 h, 48 h and 72 h. The expression of 10 important genes known to regulate hypoxia response such as HIF1A, VEGFA, EPAS1, ATP1A1, GLUT1, HMOX1, ECE1, TNF-A, GPx and SOD were evaluated in fibroblast cells of Ladakhi yak (LAY-Fb) and Sahiwal cows (SAC-Fb) during pre- and post-hypoxia stress. A panel of 10 reference genes (GAPDH, RPL4, EEF1A1, RPS9, HPRT1, UXT, RPS23, B2M, RPS15, ACTB) were also evaluated for their expression stability to perform accurate normalization. The expression of HIF1A was significantly (p < 0.05) induced in both LAY-Fb (2.29-fold) and SAC-Fb (2.07-fold) after 24 h of hypoxia stress. The angiogenic (VEGFA), metabolic (GLUT1) and antioxidant genes (SOD and GPx) were also induced after 24 h of hypoxia stress. However, EPAS1 and ATP1A1 induced significantly (p < 0.05) after 48 h whereas, ECE1 expression induced significantly (p < 0.05) at 72 h after exposure to hypoxia. The TNF-alpha which is a pro-inflammatory gene induced significantly (p < 0.05) at 24 h in SAC-Fb and at 72 h in LAY-Fb. The induction of hypoxia associated genes indicated the utility of skin derived fibroblast as cellular model to evaluate transcriptome signatures post hypoxia stress in populations adapted to diverse altitudes.

本研究旨在了解牦牛(Bos grunniens)和奶牛(Bos indicus)皮肤成纤维细胞对低氧应激的转录反应。研究人员将来自拉达克牦牛和萨希瓦尔奶牛(Bos indicus)各三个个体的六个原代成纤维细胞系分别暴露在低氧浓度下 24 小时、48 小时和 72 小时。评估了拉达克牦牛(LAY-Fb)和萨希瓦尔奶牛(SAC-Fb)成纤维细胞在缺氧应激前和应激后的 10 个已知调控缺氧反应的重要基因的表达情况,如 HIF1A、VEGFA、EPAS1、ATP1A1、GLUT1、HMOX1、ECE1、TNF-A、GPx 和 SOD。此外,还评估了 10 个参考基因(GAPDH、RPL4、EEF1A1、RPS9、HPRT1、UXT、RPS23、B2M、RPS15、ACTB)的表达稳定性,以进行准确的归一化。HIF1A 的表达明显(p
{"title":"Hypoxia related genes modulate in similar fashion in skin fibroblast cells of yak (Bos grunniens) adapted to high altitude and native cows (Bos indicus) adapted to tropical climate during hypoxia stress.","authors":"Manish Tiwari, Monika Sodhi, Manish Sharma, Vishal Sharma, Manishi Mukesh","doi":"10.1007/s00484-024-02695-5","DOIUrl":"10.1007/s00484-024-02695-5","url":null,"abstract":"<p><p>The present study was conducted to understand transcriptional response of skin fibroblast of yak (Bos grunniens) and cows of Bos indicus origin to hypoxia stress. Six primary fibroblast cell lines derived from three individuals each of Ladakhi yak (Bos grunniens) and Sahiwal cows (Bos indicus) were exposed to low oxygen concentration for a period of 24 h, 48 h and 72 h. The expression of 10 important genes known to regulate hypoxia response such as HIF1A, VEGFA, EPAS1, ATP1A1, GLUT1, HMOX1, ECE1, TNF-A, GPx and SOD were evaluated in fibroblast cells of Ladakhi yak (LAY-Fb) and Sahiwal cows (SAC-Fb) during pre- and post-hypoxia stress. A panel of 10 reference genes (GAPDH, RPL4, EEF1A1, RPS9, HPRT1, UXT, RPS23, B2M, RPS15, ACTB) were also evaluated for their expression stability to perform accurate normalization. The expression of HIF1A was significantly (p < 0.05) induced in both LAY-Fb (2.29-fold) and SAC-Fb (2.07-fold) after 24 h of hypoxia stress. The angiogenic (VEGFA), metabolic (GLUT1) and antioxidant genes (SOD and GPx) were also induced after 24 h of hypoxia stress. However, EPAS1 and ATP1A1 induced significantly (p < 0.05) after 48 h whereas, ECE1 expression induced significantly (p < 0.05) at 72 h after exposure to hypoxia. The TNF-alpha which is a pro-inflammatory gene induced significantly (p < 0.05) at 24 h in SAC-Fb and at 72 h in LAY-Fb. The induction of hypoxia associated genes indicated the utility of skin derived fibroblast as cellular model to evaluate transcriptome signatures post hypoxia stress in populations adapted to diverse altitudes.</p>","PeriodicalId":588,"journal":{"name":"International Journal of Biometeorology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141173970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphological spatial clustering of high-density central areas and their coupling relationship with thermal environment--a case study of the wuyi road hatchback in changsha. 高密度中心区的形态空间集聚及其与热环境的耦合关系--以长沙市五一路孵化园为例。
IF 3 3区 地球科学 Q2 BIOPHYSICS Pub Date : 2024-08-01 Epub Date: 2024-05-02 DOI: 10.1007/s00484-024-02687-5
Su Xijing, Liu Luyun, Yi Pei, Chen Cunyou, Zhang Minhuan

Intense urban development and high urban density cause the thermal environment in urban centers to deteriorate continuously, affecting the quality of the living environment. In this study, 707.49 hectares of land in the central area of Changsha were divided into 121 plots. 11 microclimate-related morphological indicators were comprehensively selected, and the K-means method was used for cluster analysis. Then, the relationship between morphological clusters and the thermal environment was explored by simulating the thermal environment of the study area with ENVI-met. First, five spatial types were found to characterize the area: high-level with high floor area ratio, low density, and low greenery; middle-level with high floor area ratio high density; medium-capacity with high density and small volume; low-level with low density and high greenery; and low floor area ratio, low density, and high greenery. Second, the building windward surface density, sky openness, building density, floor area ratio and green space rate affect the thermal environment. Third, Cluster3 had the highest average air temperature (Ta), followed by Cluster5, furthermore Clusters4, 1, and2 had relatively low Ta. The spatial vitality index and green space rate in Cluster1; the area-weighted building shape index, average building volume and sky openness in Cluster2; green space rate in Cluster3; indicators such as the floor area ratio and green space rate in Cluster4; indicators such as the impervious surface rate and green space rate in Cluster5 had greater influences on Ta. Fourthly, simply increasing the area of green space cannot maximize the cooling effect of green spaces. Instead, constructing an equalized greening network can better regulate the thermal environment. Fifthly, the results provide a scientific basis for the design and the regulation of urban centers.

激烈的城市发展和高密度的城市建设导致城市中心区的热环境不断恶化,影响了居住环境的质量。本研究将长沙市中心城区 707.49 公顷土地划分为 121 个地块。综合选取 11 个与小气候相关的形态指标,采用 K 均值法进行聚类分析。然后,利用 ENVI-met 模拟研究区的热环境,探讨形态聚类与热环境之间的关系。首先,研究发现了五种空间类型:高层高容积率、低密度、低绿化;中层高容积率、高密度;中层高密度、小体量;低层低密度、高绿化;低容积率、低密度、高绿化。其次,建筑迎风面密度、天空开阔度、建筑密度、容积率和绿地率都会影响热环境。第三,组团 3 的平均气温(Ta)最高,其次是组团 5,而组团 4、1 和 2 的平均气温(Ta)相对较低。组群 1 中的空间活力指数和绿地率,组群 2 中的面积加权建筑形态指数、平均建筑体量和天空开阔度,组群 3 中的绿地率,组群 4 中的容积率和绿地率等指标,组群 5 中的不透水面积率和绿地率等指标对 Ta 的影响较大。第四,单纯增加绿地面积并不能最大限度地发挥绿地的降温作用。相反,构建均衡的绿化网络可以更好地调节热环境。第五,研究结果为城市中心区的设计和调控提供了科学依据。
{"title":"Morphological spatial clustering of high-density central areas and their coupling relationship with thermal environment--a case study of the wuyi road hatchback in changsha.","authors":"Su Xijing, Liu Luyun, Yi Pei, Chen Cunyou, Zhang Minhuan","doi":"10.1007/s00484-024-02687-5","DOIUrl":"10.1007/s00484-024-02687-5","url":null,"abstract":"<p><p>Intense urban development and high urban density cause the thermal environment in urban centers to deteriorate continuously, affecting the quality of the living environment. In this study, 707.49 hectares of land in the central area of Changsha were divided into 121 plots. 11 microclimate-related morphological indicators were comprehensively selected, and the K-means method was used for cluster analysis. Then, the relationship between morphological clusters and the thermal environment was explored by simulating the thermal environment of the study area with ENVI-met. First, five spatial types were found to characterize the area: high-level with high floor area ratio, low density, and low greenery; middle-level with high floor area ratio high density; medium-capacity with high density and small volume; low-level with low density and high greenery; and low floor area ratio, low density, and high greenery. Second, the building windward surface density, sky openness, building density, floor area ratio and green space rate affect the thermal environment. Third, Cluster3 had the highest average air temperature (Ta), followed by Cluster5, furthermore Clusters4, 1, and2 had relatively low Ta. The spatial vitality index and green space rate in Cluster1; the area-weighted building shape index, average building volume and sky openness in Cluster2; green space rate in Cluster3; indicators such as the floor area ratio and green space rate in Cluster4; indicators such as the impervious surface rate and green space rate in Cluster5 had greater influences on Ta. Fourthly, simply increasing the area of green space cannot maximize the cooling effect of green spaces. Instead, constructing an equalized greening network can better regulate the thermal environment. Fifthly, the results provide a scientific basis for the design and the regulation of urban centers.</p>","PeriodicalId":588,"journal":{"name":"International Journal of Biometeorology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140849260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Temporal changes in temperature-related mortality in relation to the establishment of the heat-health alert system in Victoria, Australia. 与澳大利亚维多利亚州建立高温健康警报系统有关的温度相关死亡率的时间变化。
IF 3 3区 地球科学 Q2 BIOPHYSICS Pub Date : 2024-08-01 Epub Date: 2024-05-06 DOI: 10.1007/s00484-024-02691-9
Nicholas J Osborne, Patrick Amoatey, Linda Selvey, Dung Phung

Extreme heat alerts are the most common form of weather forecasting services used in Australia, yet very limited studies have documented their effectiveness in improving health outcomes. This study aimed to examine the temporal changes in temperature-related mortality in relation to the activation of the heat-health alert and response system (HARS) in the State of Victoria, Australia. We examined the relationship between temperatures and mortality using quasi-Poisson regression and the distributed lag non-linear model (dlnm) and compared the temperature-mortality association between the two periods: period 1- prior-HARS (1992-2009) and period 2- post-HARS (2010-2019). Since the HARS heavily weights heatwave effects, we also compared the main effects of heatwave events between the two periods. The heatwaves were defined for three levels, including 3 consecutive days at 97th, 98th, and 99th percentiles. We also controlled the potential confounding effect of seasonality by including a natural cubic B-spline of the day of the year with equally spaced knots and 8 degrees of freedom per year. The exposure-response curve reveals the temperature mortality was reduced in period 2 in comparison with period 1. The relative risk ratios (RRR) of Period 2 over Period 1 were all less than one and gradually decreased from 0.86 (95% CI, 0.72-1.03) to 0.64 (95% CI, 0.33-1.22), and the differences in attributable risk percent increased from 13.2 to 25.3%. The reduction in the risk of heatwave-related deaths decreased by 3.4% (RRp1 1.068, 95% CI, 1.024-1.112 versus RRp2 1.034, 95% CI, 0.986-1.082) and 10% (RRp1 1.16, 95% CI, 1.10-1.22 versus RRp2 1.06, 95% CI, 1.002-1.119) for all groups of people. The study indicated a decrease in heat-related mortality following the operation of HARS in Victoria under extreme heat and high-intensity heatwaves conditions. Further studies could investigate the extent of changes in mortality among populations of differing socio-economic groups during the operation of the heat-health alert system.

极热警报是澳大利亚最常用的天气预报服务形式,但记录其在改善健康状况方面效果的研究却非常有限。本研究旨在考察澳大利亚维多利亚州与高温-健康警报和响应系统(HARS)启动有关的温度相关死亡率的时间变化。我们使用准泊松回归和分布式滞后非线性模型(dlnm)研究了气温与死亡率之间的关系,并比较了两个时期气温与死亡率之间的关系:第一时期--HARS 之前(1992-2009 年)和第二时期--HARS 之后(2010-2019 年)。由于 HARS 严重加权了热浪效应,我们还比较了两个时期热浪事件的主要效应。热浪分为三个级别,包括连续 3 天的第 97、98 和 99 百分位数。我们还控制了季节性的潜在混杂效应,方法是在一年中加入等间距节的自然立方 B-样条,每年 8 个自由度。暴露-反应曲线显示,与第 1 期相比,第 2 期的温度死亡率有所降低。第 2 期与第 1 期相比,相对风险比均小于 1,并从 0.86(95% CI,0.72-1.03)逐渐下降到 0.64(95% CI,0.33-1.22),可归因风险百分比差异从 13.2% 增加到 25.3%。在所有人群中,与热浪相关的死亡风险降低了3.4%(RRP1为1.068,95% CI为1.024-1.112,RRP2为1.034,95% CI为0.986-1.082)和10%(RRP1为1.16,95% CI为1.10-1.22,RRP2为1.06,95% CI为1.002-1.119)。研究结果表明,在维多利亚州,在极端高温和高强度热浪条件下,运行 HARS 后与高温相关的死亡率有所下降。进一步的研究可以调查在高温健康警报系统运行期间,不同社会经济群体的死亡率变化程度。
{"title":"Temporal changes in temperature-related mortality in relation to the establishment of the heat-health alert system in Victoria, Australia.","authors":"Nicholas J Osborne, Patrick Amoatey, Linda Selvey, Dung Phung","doi":"10.1007/s00484-024-02691-9","DOIUrl":"10.1007/s00484-024-02691-9","url":null,"abstract":"<p><p>Extreme heat alerts are the most common form of weather forecasting services used in Australia, yet very limited studies have documented their effectiveness in improving health outcomes. This study aimed to examine the temporal changes in temperature-related mortality in relation to the activation of the heat-health alert and response system (HARS) in the State of Victoria, Australia. We examined the relationship between temperatures and mortality using quasi-Poisson regression and the distributed lag non-linear model (dlnm) and compared the temperature-mortality association between the two periods: period 1- prior-HARS (1992-2009) and period 2- post-HARS (2010-2019). Since the HARS heavily weights heatwave effects, we also compared the main effects of heatwave events between the two periods. The heatwaves were defined for three levels, including 3 consecutive days at 97th, 98th, and 99th percentiles. We also controlled the potential confounding effect of seasonality by including a natural cubic B-spline of the day of the year with equally spaced knots and 8 degrees of freedom per year. The exposure-response curve reveals the temperature mortality was reduced in period 2 in comparison with period 1. The relative risk ratios (RRR) of Period 2 over Period 1 were all less than one and gradually decreased from 0.86 (95% CI, 0.72-1.03) to 0.64 (95% CI, 0.33-1.22), and the differences in attributable risk percent increased from 13.2 to 25.3%. The reduction in the risk of heatwave-related deaths decreased by 3.4% (RR<sub>p1</sub> 1.068, 95% CI, 1.024-1.112 versus RR<sub>p2</sub> 1.034, 95% CI, 0.986-1.082) and 10% (RR<sub>p1</sub> 1.16, 95% CI, 1.10-1.22 versus RR<sub>p2</sub> 1.06, 95% CI, 1.002-1.119) for all groups of people. The study indicated a decrease in heat-related mortality following the operation of HARS in Victoria under extreme heat and high-intensity heatwaves conditions. Further studies could investigate the extent of changes in mortality among populations of differing socio-economic groups during the operation of the heat-health alert system.</p>","PeriodicalId":588,"journal":{"name":"International Journal of Biometeorology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11282152/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140850586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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International Journal of Biometeorology
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