Selection of the most stably expressed reference genes is key to monitoring accurate target gene expression across any tissue or cell type. The mRNA in spermatozoa stores valuable information related to changes in spermatogenesis due to variations in environmental conditions, especially during heat stress, which affects various sperm functions. Semen quality in buffalo bulls is significantly influenced by the seasons. In the study, a panel of nine genes was evaluated to identify the most stably expressed internal control gene (ICG) for the normalization of real-time gene expression data generated across various seasons for Murrah buffalo bulls’ spermatozoa. Sperm cells were purified from the semen samples collected during different seasons, with temperature-humidity index (THI) ranging from 80.80 ± 1.47 (hot summer) to 55.88 ± 1.98 (winter), using the BoviPure™ gradient purification method. The RNA isolated from the purified spermatozoa fraction was quality checked prior to reverse transcription and subjected to qPCR (quantitative real-time PCR) based expression analysis. An automated ‘endoGene’ pipeline was employed to apply the geNorm, NormFinder, and BestKeeper algorithms for data analysis. The result indicated that GAPDH and PP1A were the most stably expressed among the gene panel, whereas ATPSF1 and ACTB were the two least stable expressed reference genes. Further, the most suitable ICGs identified were validated by normalization of real time expression data of heat stress and sperm quality genes, HSFY2 and AKAP4, respectively. The genes identified would help in generating the most reliable results for the expression profiling of the genes dictating sperm quality and heat stress cope-up mechanism in buffalo spermatozoa, collected during different seasons.
{"title":"Selection of reference genes for normalizing gene expression data across seasons in spermatozoa of water buffalo (Bubalus bubalis)","authors":"Rashi Vasisth, Ankita Gurao, Meenakshi Chitkara, Gautam Kumar, Karpenahalli Ranganatha Sriranga, Manishi Mukesh, Mahesh Shivanand Dige, Pawan Singh, Rajeev Anand Kumar Aggarwal, Ranjit Singh Kataria","doi":"10.1007/s00484-024-02675-9","DOIUrl":"https://doi.org/10.1007/s00484-024-02675-9","url":null,"abstract":"<p>Selection of the most stably expressed reference genes is key to monitoring accurate target gene expression across any tissue or cell type. The mRNA in spermatozoa stores valuable information related to changes in spermatogenesis due to variations in environmental conditions, especially during heat stress, which affects various sperm functions. Semen quality in buffalo bulls is significantly influenced by the seasons. In the study, a panel of nine genes was evaluated to identify the most stably expressed internal control gene (ICG) for the normalization of real-time gene expression data generated across various seasons for Murrah buffalo bulls’ spermatozoa. Sperm cells were purified from the semen samples collected during different seasons, with temperature-humidity index (THI) ranging from 80.80 ± 1.47 (hot summer) to 55.88 ± 1.98 (winter), using the BoviPure™ gradient purification method. The RNA isolated from the purified spermatozoa fraction was quality checked prior to reverse transcription and subjected to qPCR (quantitative real-time PCR) based expression analysis. An automated ‘endoGene’ pipeline was employed to apply the geNorm, NormFinder, and BestKeeper algorithms for data analysis. The result indicated that <i>GAPDH</i> and <i>PP1A</i> were the most stably expressed among the gene panel, whereas <i>ATPSF1</i> and <i>ACTB</i> were the two least stable expressed reference genes. Further, the most suitable ICGs identified were validated by normalization of real time expression data of heat stress and sperm quality genes, <i>HSFY2</i> and <i>AKAP4</i>, respectively. The genes identified would help in generating the most reliable results for the expression profiling of the genes dictating sperm quality and heat stress cope-up mechanism in buffalo spermatozoa, collected during different seasons.</p><h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>","PeriodicalId":588,"journal":{"name":"International Journal of Biometeorology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140563151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-11DOI: 10.1007/s00484-024-02676-8
Josiel Ferreira, Elisomar André da Silva, Robson Mateus Freitas Silveira, José Ernandes Rufino de Sousa, Ricardo Lopes Dias da Costa, Concepta Margaret McManus, Débora Andréa Evangelista Façanha
Coat color is a factor affecting heat tolerance in tropical ruminant and a particular coat color can determine which is more resilient to environmental changes. The aim of this study was to measure the level of adaptation of Morada Nova sheep with different coat color by using an Adaptability Index (AI). Adult ewes were used, including two different coat colors of Morada Nova sheep (red and white) with mean of body weight of 28.02 ± 5.70 kg and 31.47 ± 3.41 kg, respectively. Physiology parameters, hematology, electrolytes, acid–base status, mineral, renal functions, metabolites, enzymes, and proteins were measured. AI was designed using a multivariate approach (principal component analysis) to "weigh" the influence of each variable in the animal responses. The variables more important for adaptive aspects of Red Morada Nova were: haematology, electrolytes and acid–base status. The hemoglobin (HG), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), sodium (Na+), oxygen pressure (PO2), glucose (GLU) and albumin (ALB) were significantly higher in Red Morada Nova sheep and hydrogen carbonate (HCO3), base excess (BE), total carbon dioxide concentration (TCO2) and URE were significantly higher in the white phenotype. The variables more important for adaptive aspects of White Morada Nova sheep were: (K+), total protein (TP), PO2, HG, cholesterol (CHO), rectal temperature (RT) and glucose (GLU). Both phenotypes showed a high adaptation level, however, a higher value was generated for the Red Morada Nova sheep (81.97). This study concludes that both phenotypes of the Morada Nova sheep breed are well adapted to the climatic condition of the Brazilian tropical region using different adaptive mechanisms.
被毛颜色是影响热带反刍动物耐热性的一个因素,特定的被毛颜色可以决定哪种反刍动物对环境变化的适应能力更强。本研究的目的是利用适应性指数(AI)测量不同毛色的莫拉达诺瓦羊的适应性水平。研究对象为成年母羊,包括两种不同毛色的 Morada Nova 羊(红色和白色),平均体重分别为 28.02 ± 5.70 千克和 31.47 ± 3.41 千克。对生理参数、血液学、电解质、酸碱状态、矿物质、肾功能、代谢物、酶和蛋白质进行了测量。采用多变量方法(主成分分析)设计人工智能,以 "权衡 "每个变量对动物反应的影响。对 Red Morada Nova 的适应性更重要的变量是:血液学、电解质和酸碱状态。红色 Morada Nova 羊的血红蛋白 (HG)、平均血红蛋白 (MCH)、平均血红蛋白浓度 (MCHC)、钠 (Na+)、氧压 (PO2)、葡萄糖 (GLU) 和白蛋白 (ALB) 明显较高,而白色表型羊的碳酸氢盐 (HCO3)、碱过量 (BE)、总二氧化碳浓度 (TCO2) 和脲酸水平则明显较高。对白莫拉达新星绵羊的适应性更重要的变量是(K+)、总蛋白(TP)、PO2、HG、胆固醇(CHO)、直肠温度(RT)和葡萄糖(GLU)。两种表型都显示出较高的适应水平,但红色 Morada Nova 羊的适应值更高(81.97)。这项研究的结论是,莫拉达诺瓦绵羊品种的两种表型都能很好地适应巴西热带地区的气候条件,并采用了不同的适应机制。
{"title":"Assessment of the adaptive capacity of Morada Nova ewes with different coat coloration","authors":"Josiel Ferreira, Elisomar André da Silva, Robson Mateus Freitas Silveira, José Ernandes Rufino de Sousa, Ricardo Lopes Dias da Costa, Concepta Margaret McManus, Débora Andréa Evangelista Façanha","doi":"10.1007/s00484-024-02676-8","DOIUrl":"https://doi.org/10.1007/s00484-024-02676-8","url":null,"abstract":"<p>Coat color is a factor affecting heat tolerance in tropical ruminant and a particular coat color can determine which is more resilient to environmental changes. The aim of this study was to measure the level of adaptation of Morada Nova sheep with different coat color by using an Adaptability Index (AI). Adult ewes were used, including two different coat colors of Morada Nova sheep (red and white) with mean of body weight of 28.02 ± 5.70 kg and 31.47 ± 3.41 kg, respectively. Physiology parameters, hematology, electrolytes, acid–base status, mineral, renal functions, metabolites, enzymes, and proteins were measured. AI was designed using a multivariate approach (principal component analysis) to \"weigh\" the influence of each variable in the animal responses. The variables more important for adaptive aspects of Red Morada Nova were: haematology, electrolytes and acid–base status. The hemoglobin (HG), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), sodium (Na<sup>+</sup>), oxygen pressure (PO<sub>2</sub>), glucose (GLU) and albumin (ALB) were significantly higher in Red Morada Nova sheep and hydrogen carbonate (HCO<sup>3</sup>), base excess (BE), total carbon dioxide concentration (TCO<sub>2</sub>) and URE were significantly higher in the white phenotype. The variables more important for adaptive aspects of White Morada Nova sheep were: (K<sup>+</sup>), total protein (TP), PO<sub>2</sub>, HG, cholesterol (CHO), rectal temperature (RT) and glucose (GLU). Both phenotypes showed a high adaptation level, however, a higher value was generated for the Red Morada Nova sheep (81.97). This study concludes that both phenotypes of the Morada Nova sheep breed are well adapted to the climatic condition of the Brazilian tropical region using different adaptive mechanisms.</p>","PeriodicalId":588,"journal":{"name":"International Journal of Biometeorology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140562970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-10DOI: 10.1007/s00484-024-02667-9
Naoyuki Yamashita, Masashi Kume, Toshiyuki Satake, Keiko Inoue, Tetsuya Yoshida
Associations of exertional heat exhaustion (EHE)-related symptoms with lifestyle habits and health factors specific to female athletes, ranked by relative risks from high to low, remain elusive. Addressing this issue would benefit athletes and coaches, ensuring safer practices during summer sports activities. To address this issue, we distributed paper-based questionnaires to seven universities in Japan, and 983 respondents completed our survey. The questionnaires covered various personal characteristics, lifestyle habits, perceived health factors, perceived athletic activity, and EHE-related symptoms. In this retrospective case–control study, we analyzed the relationships of EHE-related symptoms (objective variables) with lifestyle habits, health factors, and athletic activity conditions (explanatory variables) using the partial proportional odds model. The questionnaire responses revealed that perceived dehydration, sickness, loss of appetite, perceived accumulated fatigue, perceived mental stress, lack of ambient wind, and insufficient rest breaks were positively associated with EHE-related symptoms, with relative risks ranging from high to low. Using an air conditioner during sleep and having a sleep duration of ≥ 6 h were associated with a reduced risk of EHE-related symptoms. The study results suggest that female athletes should be allowed to postpone exercise or reduce its intensity and volume based on their perceptions of dehydration, sickness, loss of appetite, accumulated fatigue, and mental stress in summer to prevent heat-related illnesses.
{"title":"Subjective perceived risk factors of exertional heat exhaustion-related symptoms in female collegiate athletes in Japan","authors":"Naoyuki Yamashita, Masashi Kume, Toshiyuki Satake, Keiko Inoue, Tetsuya Yoshida","doi":"10.1007/s00484-024-02667-9","DOIUrl":"https://doi.org/10.1007/s00484-024-02667-9","url":null,"abstract":"<p>Associations of exertional heat exhaustion (EHE)-related symptoms with lifestyle habits and health factors specific to female athletes, ranked by relative risks from high to low, remain elusive. Addressing this issue would benefit athletes and coaches, ensuring safer practices during summer sports activities. To address this issue, we distributed paper-based questionnaires to seven universities in Japan, and 983 respondents completed our survey. The questionnaires covered various personal characteristics, lifestyle habits, perceived health factors, perceived athletic activity, and EHE-related symptoms. In this retrospective case–control study, we analyzed the relationships of EHE-related symptoms (objective variables) with lifestyle habits, health factors, and athletic activity conditions (explanatory variables) using the partial proportional odds model. The questionnaire responses revealed that perceived dehydration, sickness, loss of appetite, perceived accumulated fatigue, perceived mental stress, lack of ambient wind, and insufficient rest breaks were positively associated with EHE-related symptoms, with relative risks ranging from high to low. Using an air conditioner during sleep and having a sleep duration of ≥ 6 h were associated with a reduced risk of EHE-related symptoms. The study results suggest that female athletes should be allowed to postpone exercise or reduce its intensity and volume based on their perceptions of dehydration, sickness, loss of appetite, accumulated fatigue, and mental stress in summer to prevent heat-related illnesses.</p>","PeriodicalId":588,"journal":{"name":"International Journal of Biometeorology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140563144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-07DOI: 10.1007/s00484-024-02659-9
Abstract
Meteorological variables are essential inputs for agricultural simulation models and the lack of measured data is a big challenge for the application of these models in many agricultural zones. Studies indicated that gridded meteorological datasets can be proper replacements for measured data. This paper aimed to examine a new gridded meteorological dataset namely CRU-JRA for crop modeling intents. The CRU-JRA is a 6-hourly dataset with a spatial resolution of 0.5° × 0.5° that was primarily constructed for modeling purposes. The CERES-Wheat model in the Decision Support System for Agrotechnology Transfer (DSSAT) was used for the simulation of irrigated and rainfed wheat production systems in Iran. Results showed that the CRU-JRA maximum and minimum temperature values had a relatively fine accuracy with a normalized root mean square error (NRMSE) of 14% for the simulated grain yield. The performance of the CRU-JRA solar radiation values for the simulation of grain yield was similar with a NRMSE of 14.4%. The weakest performance was found for the CRU-JRA precipitation values with a NRMSE of 18.9%. Overall, the CRU-JRA dataset performed comparatively acceptable and similar to existing gridded meteorological datasets for crop modeling purposes in the study area, however further calibrations can improve the accuracy of the next versions of this dataset. More research is necessary for the investigation of the CRU-JRA dataset for agricultural modeling purposes across diverse climates.
{"title":"Evaluation of CRU-JRA gridded meteorological dataset for modeling of wheat production systems in Iran","authors":"","doi":"10.1007/s00484-024-02659-9","DOIUrl":"https://doi.org/10.1007/s00484-024-02659-9","url":null,"abstract":"<h3>Abstract</h3> <p>Meteorological variables are essential inputs for agricultural simulation models and the lack of measured data is a big challenge for the application of these models in many agricultural zones. Studies indicated that gridded meteorological datasets can be proper replacements for measured data. This paper aimed to examine a new gridded meteorological dataset namely CRU-JRA for crop modeling intents. The CRU-JRA is a 6-hourly dataset with a spatial resolution of 0.5° × 0.5° that was primarily constructed for modeling purposes. The CERES-Wheat model in the Decision Support System for Agrotechnology Transfer (DSSAT) was used for the simulation of irrigated and rainfed wheat production systems in Iran. Results showed that the CRU-JRA maximum and minimum temperature values had a relatively fine accuracy with a normalized root mean square error (NRMSE) of 14% for the simulated grain yield. The performance of the CRU-JRA solar radiation values for the simulation of grain yield was similar with a NRMSE of 14.4%. The weakest performance was found for the CRU-JRA precipitation values with a NRMSE of 18.9%. Overall, the CRU-JRA dataset performed comparatively acceptable and similar to existing gridded meteorological datasets for crop modeling purposes in the study area, however further calibrations can improve the accuracy of the next versions of this dataset. More research is necessary for the investigation of the CRU-JRA dataset for agricultural modeling purposes across diverse climates.</p>","PeriodicalId":588,"journal":{"name":"International Journal of Biometeorology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140562992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Primary gaseous air pollutants have been associated with death from multiple causes, however, it remains unknown if they play a role in premature mortality among individuals living with HIV/AIDS. Data on HIV/AIDS patients were collected from the Hubei Provincial Center for Disease Control and Prevention, with a total of 1,467 AIDS-related deaths (ARD) between 2013 and 2020. Daily mean sulfur dioxide (SO2), nitrogen dioxide (NO2), and carbon monoxide (CO) were generated by artificial intelligence algorithms combined with big data. We employed a time-stratified case-crossover approach and conditional logistical regression models to investigate the acute effects of primary gaseous air pollutants on ARD. Per interquartile range increase in the concentrations of SO2 was significantly linked with ARD, with a corresponding odds ratio (OR) of 1.17 [95% confidence intervals (CIs): 1.01, 1.35] at lag 4 day. Furthermore, our findings indicated that males exhibited a heightened vulnerability to the adverse effects of SO2 and NO2, for example, the ORs were 1.24 (95% CIs: 1.05, 1.47) and 1.16 (95% CIs: 1.01, 1.34), respectively. Moreover, individuals aged over 65 years were more susceptible to SO2 and CO. Additionally, we identified the warm season as a sensitive period for mortality associated with SO2 and NO2. Our study furnished fresh evidence regarding the detrimental effects of primary gaseous air pollutants on ARD.
{"title":"Is short-term exposure to primary gaseous air pollutants associated with AIDS-related deaths? Evidence from a time-stratified case-crossover study","authors":"Dan Sun, Faxue Zhang, Lianguo Ruan, Dingyuan Zhao, Heng Tang, Wei Zhu","doi":"10.1007/s00484-024-02664-y","DOIUrl":"https://doi.org/10.1007/s00484-024-02664-y","url":null,"abstract":"<p>Primary gaseous air pollutants have been associated with death from multiple causes, however, it remains unknown if they play a role in premature mortality among individuals living with HIV/AIDS. Data on HIV/AIDS patients were collected from the Hubei Provincial Center for Disease Control and Prevention, with a total of 1,467 AIDS-related deaths (ARD) between 2013 and 2020. Daily mean sulfur dioxide (SO<sub>2</sub>), nitrogen dioxide (NO<sub>2</sub>), and carbon monoxide (CO) were generated by artificial intelligence algorithms combined with big data. We employed a time-stratified case-crossover approach and conditional logistical regression models to investigate the acute effects of primary gaseous air pollutants on ARD. Per interquartile range increase in the concentrations of SO<sub>2</sub> was significantly linked with ARD, with a corresponding odds ratio (OR) of 1.17 [95% confidence intervals (CIs): 1.01, 1.35] at lag 4 day. Furthermore, our findings indicated that males exhibited a heightened vulnerability to the adverse effects of SO<sub>2</sub> and NO<sub>2</sub>, for example, the ORs were 1.24 (95% CIs: 1.05, 1.47) and 1.16 (95% CIs: 1.01, 1.34), respectively. Moreover, individuals aged over 65 years were more susceptible to SO<sub>2</sub> and CO. Additionally, we identified the warm season as a sensitive period for mortality associated with SO<sub>2</sub> and NO<sub>2</sub>. Our study furnished fresh evidence regarding the detrimental effects of primary gaseous air pollutants on ARD.</p>","PeriodicalId":588,"journal":{"name":"International Journal of Biometeorology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140563042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.1007/s00484-024-02663-z
Meng Du, Yanbo Wang, Yinuo Zhang, Xiaohui Nian, Bo Hong
We recruited 162 healthy elderly adults to determine the thermal, physiological, and psychological effects of horticultural activities (flower arranging, transplanting, and rubble masonry) in outdoor open spaces. We linked these to local climate conditions, physiology, and comfort through a questionnaire survey. The results showed that: (1) the neutral physiological equivalent temperature (NPET) before the horticultural activities were 22.18 ℃ for flower arranging, 23.67 ℃ for transplanting, and 20.78 ℃ for rubble masonry, while the NPET decreased to 18.53 ℃, 20.73 ℃ and 18.04 ℃ (respectively) after activities. (2) The heart rate and blood oxygen saturation changed significantly (p < 0.05) only after rubble masonry. (3) The average positive affect (PA) scores increased after flower arranging by 4.83, transplanting by 3.30, and rubble masonry by 4.00. (4) After activities, the thermal sensation vote was mainly influenced by globe temperature (41.36%), air temperature (33.47%), and wind speed (25.17%). Thermal comfort vote could be promoted because of 37.35% of an increasing positive and 21.20% of decreasing negative emotion.
{"title":"How outdoor horticultural activities affect elderly adults' thermal, physiological and psychological responses: a field study.","authors":"Meng Du, Yanbo Wang, Yinuo Zhang, Xiaohui Nian, Bo Hong","doi":"10.1007/s00484-024-02663-z","DOIUrl":"https://doi.org/10.1007/s00484-024-02663-z","url":null,"abstract":"<p><p>We recruited 162 healthy elderly adults to determine the thermal, physiological, and psychological effects of horticultural activities (flower arranging, transplanting, and rubble masonry) in outdoor open spaces. We linked these to local climate conditions, physiology, and comfort through a questionnaire survey. The results showed that: (1) the neutral physiological equivalent temperature (NPET) before the horticultural activities were 22.18 ℃ for flower arranging, 23.67 ℃ for transplanting, and 20.78 ℃ for rubble masonry, while the NPET decreased to 18.53 ℃, 20.73 ℃ and 18.04 ℃ (respectively) after activities. (2) The heart rate and blood oxygen saturation changed significantly (p < 0.05) only after rubble masonry. (3) The average positive affect (PA) scores increased after flower arranging by 4.83, transplanting by 3.30, and rubble masonry by 4.00. (4) After activities, the thermal sensation vote was mainly influenced by globe temperature (41.36%), air temperature (33.47%), and wind speed (25.17%). Thermal comfort vote could be promoted because of 37.35% of an increasing positive and 21.20% of decreasing negative emotion.</p>","PeriodicalId":588,"journal":{"name":"International Journal of Biometeorology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140334208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Meteorological factors and air pollutants are associated with the spread of pulmonary tuberculosis (PTB), but few studies have examined the effects of their interactions on PTB. Therefore, this study investigated the impact of meteorological factors and air pollutants and their interactions on the risk of PTB in Urumqi, a city with a high prevalence of PTB and a high level of air pollution. The number of new PTB cases in eight districts of Urumqi from 2014 to 2019 was collected, along with data on meteorological factors and air pollutants for the same period. A generalized additive model was applied to explore the effects of meteorological factors and air pollutants and their interactions on the risk of PTB incidence. Segmented linear regression was used to estimate the nonlinear characteristics of the impact of meteorological factors on PTB. During 2014-2019, a total of 14,402 new cases of PTB were reported in eight districts, with March to May being the months of high PTB incidence. The exposure-response curves for temperature (Temp), relative humidity (RH), wind speed (WS), air pressure (AP), and diurnal temperature difference (DTR) were generally inverted "U" shaped, with the corresponding threshold values of - 5.411 °C, 52.118%, 3.513 m/s, 1021.625 hPa, and 8.161 °C, respectively. The effects of air pollutants on PTB were linear and lagged. All air pollutants were positively associated with PTB, except for O3, which was not associated with PTB, and the ER values for the effects on PTB were as follows: 0.931 (0.255, 1.612) for PM2.5, 1.028 (0.301, 1.760) for PM10, 5.061 (0.387, 9.952) for SO2, 2.830 (0.512, 5.200) for NO2, and 5.789 (1.508, 10.251) for CO. Meteorological factors and air pollutants have an interactive effect on PTB. The risk of PTB incidence was higher when in high Temp-high air pollutant, high RH-high air pollutant, high WS-high air pollutant, lowAP-high air pollutant, and high DTR-high air pollutant. In conclusion, both meteorological and pollutant factors had an influence on PTB, and the influence on PTB may have an interaction.
{"title":"Interaction between air pollutants and meteorological factors on pulmonary tuberculosis in northwest China: A case study of eight districts in Urumqi.","authors":"Yanwu Nie, Zhen Yang, Yaoqin Lu, Mailiman Bahani, Yanling Zheng, Maozai Tian, Liping Zhang","doi":"10.1007/s00484-023-02615-z","DOIUrl":"10.1007/s00484-023-02615-z","url":null,"abstract":"<p><p>Meteorological factors and air pollutants are associated with the spread of pulmonary tuberculosis (PTB), but few studies have examined the effects of their interactions on PTB. Therefore, this study investigated the impact of meteorological factors and air pollutants and their interactions on the risk of PTB in Urumqi, a city with a high prevalence of PTB and a high level of air pollution. The number of new PTB cases in eight districts of Urumqi from 2014 to 2019 was collected, along with data on meteorological factors and air pollutants for the same period. A generalized additive model was applied to explore the effects of meteorological factors and air pollutants and their interactions on the risk of PTB incidence. Segmented linear regression was used to estimate the nonlinear characteristics of the impact of meteorological factors on PTB. During 2014-2019, a total of 14,402 new cases of PTB were reported in eight districts, with March to May being the months of high PTB incidence. The exposure-response curves for temperature (Temp), relative humidity (RH), wind speed (WS), air pressure (AP), and diurnal temperature difference (DTR) were generally inverted \"U\" shaped, with the corresponding threshold values of - 5.411 °C, 52.118%, 3.513 m/s, 1021.625 hPa, and 8.161 °C, respectively. The effects of air pollutants on PTB were linear and lagged. All air pollutants were positively associated with PTB, except for O<sub>3</sub>, which was not associated with PTB, and the ER values for the effects on PTB were as follows: 0.931 (0.255, 1.612) for PM<sub>2.5</sub>, 1.028 (0.301, 1.760) for PM<sub>10</sub>, 5.061 (0.387, 9.952) for SO<sub>2</sub>, 2.830 (0.512, 5.200) for NO<sub>2</sub>, and 5.789 (1.508, 10.251) for CO. Meteorological factors and air pollutants have an interactive effect on PTB. The risk of PTB incidence was higher when in high Temp-high air pollutant, high RH-high air pollutant, high WS-high air pollutant, lowAP-high air pollutant, and high DTR-high air pollutant. In conclusion, both meteorological and pollutant factors had an influence on PTB, and the influence on PTB may have an interaction.</p>","PeriodicalId":588,"journal":{"name":"International Journal of Biometeorology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139105727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01Epub Date: 2024-01-08DOI: 10.1007/s00484-023-02611-3
Kelsey N Ellis, Jennifer M First, Kristina W Kintziger, Ella Hunter
Nighttime heat is an important factor in heat-health outcomes, though nighttime heat exposure and its impacts are poorly understood. We assessed overnight heat in indoor (n = 12) and outdoor (n = 3) living spaces in Knoxville, Tennessee, using iButton Hygrochrons in August 2021. Indoor sleep spaces, all of which were air conditioned, reported a variety of overnight conditions. Indoor sleep spaces were both warmer and cooler than outdoor temperatures overnight, and some participants noted having physical health effects of overnight heat in their homes. Downtown outdoor sleep spaces, including a park and encampment, exhibited an urban heat island signal, staying warmer than other outdoor areas. Future research should focus on the intensity and length of the overnight recovery period for individuals and how that affects heat-health outcomes, especially after being exposed to daytime heat. Specifically, do homes reach a cool enough temperature for recovery, and do outdoor sleeping spaces offer a long enough and cool enough period for recovery? We provide some recommendations for such future studies, including (1) focus on purposeful sampling, (2) use deliberate sensor placement for representative results, (3) prepare for participant drop-off due to non-compliance and technological problems, and (4) strategically gather demographic information.
{"title":"Overnight heat in sleep spaces of housed and unhoused residents: results and recommendations from a Knoxville, Tennessee, case study.","authors":"Kelsey N Ellis, Jennifer M First, Kristina W Kintziger, Ella Hunter","doi":"10.1007/s00484-023-02611-3","DOIUrl":"10.1007/s00484-023-02611-3","url":null,"abstract":"<p><p>Nighttime heat is an important factor in heat-health outcomes, though nighttime heat exposure and its impacts are poorly understood. We assessed overnight heat in indoor (n = 12) and outdoor (n = 3) living spaces in Knoxville, Tennessee, using iButton Hygrochrons in August 2021. Indoor sleep spaces, all of which were air conditioned, reported a variety of overnight conditions. Indoor sleep spaces were both warmer and cooler than outdoor temperatures overnight, and some participants noted having physical health effects of overnight heat in their homes. Downtown outdoor sleep spaces, including a park and encampment, exhibited an urban heat island signal, staying warmer than other outdoor areas. Future research should focus on the intensity and length of the overnight recovery period for individuals and how that affects heat-health outcomes, especially after being exposed to daytime heat. Specifically, do homes reach a cool enough temperature for recovery, and do outdoor sleeping spaces offer a long enough and cool enough period for recovery? We provide some recommendations for such future studies, including (1) focus on purposeful sampling, (2) use deliberate sensor placement for representative results, (3) prepare for participant drop-off due to non-compliance and technological problems, and (4) strategically gather demographic information.</p>","PeriodicalId":588,"journal":{"name":"International Journal of Biometeorology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139376984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01Epub Date: 2024-01-22DOI: 10.1007/s00484-024-02623-7
Katarzyna Lindner-Cendrowska, Magdalena Kuchcik
Wind speed is an important variable in the assessment of thermal comfort. Different types of meteorological devices provide different accuracy of air velocity (va) measurements, which under limited air flow conditions, may result in a discrepancy in actual thermal stress level. Simultaneous measurements on warm summer days, performed with a cup anemometer and hot-wire probe, prove that too high starting threshold of the first of these sensors can lead to a discrepancy of actual wind speed, and as a consequence can distort MRT (estimated with globe thermometers) and PET values on average up to 10 °C and 1 °C, respectively.
风速是热舒适度评估中的一个重要变量。不同类型的气象设备提供的风速测量精度不同,在有限的气流条件下,可能导致实际热应力水平的差异。在温暖的夏日,使用杯式风速计和热线探头进行的同步测量证明,其中第一种传感器的起始阈值过高会导致实际风速出现偏差,因此会扭曲 MRT(使用球形温度计估算)和 PET 值,平均分别高达 10 °C 和 1 °C。
{"title":"The impact of wind speed measurement method on MRT and PET values in limited air flow conditions on warm, sunny days.","authors":"Katarzyna Lindner-Cendrowska, Magdalena Kuchcik","doi":"10.1007/s00484-024-02623-7","DOIUrl":"10.1007/s00484-024-02623-7","url":null,"abstract":"<p><p>Wind speed is an important variable in the assessment of thermal comfort. Different types of meteorological devices provide different accuracy of air velocity (va) measurements, which under limited air flow conditions, may result in a discrepancy in actual thermal stress level. Simultaneous measurements on warm summer days, performed with a cup anemometer and hot-wire probe, prove that too high starting threshold of the first of these sensors can lead to a discrepancy of actual wind speed, and as a consequence can distort MRT (estimated with globe thermometers) and PET values on average up to 10 °C and 1 °C, respectively.</p>","PeriodicalId":588,"journal":{"name":"International Journal of Biometeorology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139511377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01Epub Date: 2024-02-19DOI: 10.1007/s00484-024-02624-6
Prabhat Kumar, Vijay K Bharti, Krishna Kumar
The exposure to high altitude and cold stress poses challenges in maintaining normal physiological standards and body homeostasis in non-native animals. To enhance our understanding of the physiology of native and non-native goats in high-altitude environments, we conducted a comparative study to examine the impact of natural hypoxic and cold stress conditions on their feed intake (FIT) and associated changes in physiological responses, including plasma glucose concentration (PGC). The study took place at an altitude of 3505.2 m above mean sea level and involved twenty-two healthy females from two different breeds of goats. This study was conducted over a period of 56 days after the arrival of non-native Black Bengal goats (BBN) and compared with native Changthangi (CHAN) goats. Both groups were extensively reared in a natural high-altitude and cold-stress environment in Leh, India, and were subjected to defined housing and management practices. The parameters evaluated included FIT, PGC, respiration rate, heart rate, pulse rate, and rectal temperature. High altitudes had a significant (p < 0.05) impact on FIT, PGC, respiration rate, heart rate, pulse rate, and rectal temperature in BBN, whereas these parameters remained stable in CHAN throughout the study period. Additionally, the detrimental effects of high-altitude stress were more pronounced in non-native goats compared to native goats. These findings suggest that physiological responses in non-native goats tend to stabilize after an initial period of adverse effects in high-altitude environments. Based on the physiological responses and glucose concentration, it is recommended to pay special attention to the nutrition of non-native goats for up to the third week (21 days) after their arrival in high-altitude areas.
{"title":"Effect of short-term exposure to high-altitude hypoxic climate on feed-intake, blood glucose level and physiological responses of native and non-native goat.","authors":"Prabhat Kumar, Vijay K Bharti, Krishna Kumar","doi":"10.1007/s00484-024-02624-6","DOIUrl":"10.1007/s00484-024-02624-6","url":null,"abstract":"<p><p>The exposure to high altitude and cold stress poses challenges in maintaining normal physiological standards and body homeostasis in non-native animals. To enhance our understanding of the physiology of native and non-native goats in high-altitude environments, we conducted a comparative study to examine the impact of natural hypoxic and cold stress conditions on their feed intake (FIT) and associated changes in physiological responses, including plasma glucose concentration (PGC). The study took place at an altitude of 3505.2 m above mean sea level and involved twenty-two healthy females from two different breeds of goats. This study was conducted over a period of 56 days after the arrival of non-native Black Bengal goats (BBN) and compared with native Changthangi (CHAN) goats. Both groups were extensively reared in a natural high-altitude and cold-stress environment in Leh, India, and were subjected to defined housing and management practices. The parameters evaluated included FIT, PGC, respiration rate, heart rate, pulse rate, and rectal temperature. High altitudes had a significant (p < 0.05) impact on FIT, PGC, respiration rate, heart rate, pulse rate, and rectal temperature in BBN, whereas these parameters remained stable in CHAN throughout the study period. Additionally, the detrimental effects of high-altitude stress were more pronounced in non-native goats compared to native goats. These findings suggest that physiological responses in non-native goats tend to stabilize after an initial period of adverse effects in high-altitude environments. Based on the physiological responses and glucose concentration, it is recommended to pay special attention to the nutrition of non-native goats for up to the third week (21 days) after their arrival in high-altitude areas.</p>","PeriodicalId":588,"journal":{"name":"International Journal of Biometeorology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139904711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}