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A Unified Motion Generation Approach for Quadruped L-S Walk and Trot Gaits Based on Linear Model Predictive Control 基于线性模型预测控制的四足 L-S 步行和小跑步态统一运动生成方法
IF 4.9 3区 计算机科学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2024-05-14 DOI: 10.1007/s42235-024-00533-3
Yapeng Shi, Zhicheng He, Xiaokun Leng, Songhao Piao, Lining Sun

The goal of this paper is to develop a unified online motion generation scheme for quadruped lateral-sequence walk and trot gaits based on a linear model predictive control formulation. Specifically, the dynamics of the linear pendulum model is formulated over a predictive horizon by dimensional analysis. Through gait pattern conversion, the lateral-sequence walk and trot gaits of the quadruped can be regarded as unified biped gaits, allowing the dynamics of the linear inverted pendulum model to serve quadruped motion generation. In addition, a simple linearization of the center of pressure constraints for these quadruped gaits is developed for linear model predictive control problem. Furthermore, the motion generation problem can be solved online by quadratic programming with foothold adaptation. It is demonstrated that the proposed unified scheme can generate stable locomotion online for quadruped lateral-sequence walk and trot gaits, both in simulation and on hardware. The results show significant performance improvements compared to previous work. Moreover, the results also suggest the linearly simplified scheme has the ability to robustness against unexpected disturbances.

本文的目标是基于线性模型预测控制公式,为四足动物的侧序行走和小跑步态开发一种统一的在线运动生成方案。具体来说,通过维度分析在预测范围内制定线性摆模型的动力学。通过步态模式转换,四足动物的侧序行走和小跑步态可被视为统一的双足步态,从而使线性倒立摆模型的动力学能够服务于四足动物的运动生成。此外,还为这些四足步态的压力中心约束条件开发了一种简单的线性化方法,用于线性模型预测控制问题。此外,运动生成问题还可以通过带有立足点自适应的二次编程在线解决。实验证明,无论是在仿真还是在硬件上,所提出的统一方案都能为四足动物的侧序行走和小跑步态在线生成稳定的运动。结果表明,与之前的工作相比,性能有了显著提高。此外,研究结果还表明,线性简化方案具有抵御意外干扰的能力。
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引用次数: 0
Application Analysis of Multiple Neurons Connected with Fast Inhibitory Synapses 连接快速抑制性突触的多个神经元的应用分析
IF 4.9 3区 计算机科学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2024-05-14 DOI: 10.1007/s42235-024-00525-3
Wen Duan, Weihai Chen, Jianhua Wang, Zhongcai Pei, Jingmeng Liu, Jianer Chen

Almost all living organisms exhibit autonomic oscillatory activities, which are primarily generated by the rhythmic activities of their neural systems. Several nonlinear oscillator models have been proposed to elucidate these neural behaviors and subsequently applied to the domain of robot control. However, the oscillation patterns generated by these models are often unpredictable and need to be obtained through parameter search. This study introduces a mathematical model that can be used to analyze multiple neurons connected through fast inhibitory synapses. The characteristic of this oscillator is that its stationary point is stable, but the location of the stationary point changes with the system state. Only through reasonable topology and threshold parameter selection can the oscillation be sustained. This study analyzed the conditions for stable oscillation in two-neuron networks and three-neuron networks, and obtained the basic rules of the phase relationship of the oscillator network established by this model. In addition, this study also introduces synchronization mechanisms into the model to enable it to be synchronized with the sensing pulse. Finally, this study used these theories to establish a robot single leg joint angle generation system. The experimental results showed that the simulated robot could achieve synchronization with human motion, and had better control effects compared to traditional oscillators.

几乎所有生物体都表现出自主振荡活动,这些活动主要由其神经系统的节律活动产生。为了阐明这些神经行为,人们提出了一些非线性振荡器模型,并随后将其应用于机器人控制领域。然而,这些模型产生的振荡模式往往是不可预测的,需要通过参数搜索来获得。本研究介绍了一种数学模型,可用于分析通过快速抑制性突触连接的多个神经元。该振荡器的特点是其静止点是稳定的,但静止点的位置会随着系统状态的变化而变化。只有通过合理的拓扑结构和阈值参数选择,才能维持振荡。本研究分析了双神经元网络和三神经元网络中稳定振荡的条件,并获得了由该模型建立的振荡器网络相位关系的基本规律。此外,本研究还在模型中引入了同步机制,使其能够与感应脉冲同步。最后,本研究利用这些理论建立了机器人单腿关节角度生成系统。实验结果表明,模拟机器人能实现与人类运动的同步,与传统振荡器相比具有更好的控制效果。
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引用次数: 0
Bioinspired Scraper-File Type Frequency-Doubling Ultrasonic Exciter 受生物启发的刮刀锉式倍频超声波激励器
IF 4.9 3区 计算机科学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2024-05-14 DOI: 10.1007/s42235-024-00518-2
Wenshuai Wu, Mingshuo Zhang, Zeming Li, Guang Yao, Xinggang Jiang, Deyuan Zhang

In the natural world, leaf-cutting ants cause vibrations through their mutual scraping of file-scraper organs. In this study, we designed a Biomimetic Ultrasonic Exciter (BUE) that imitates leaf-cutting ants. The operating characteristics of the BUE were studied through experimental testing and finite element simulations. The results showed that the BUE could generate stable ultrasonic vibrations, and that the excitation frequency only needed to be half the Output Frequency (OF). This frequency-doubling phenomenon was conducive to achieving BUE miniaturization. To further explore the phenomenon of frequency-doubling vibration output, this study designed scrapers of five different sizes, conducted excitation and first-order natural frequency measurement tests, and the corresponding finite element simulations. It was found that each scraper could operate in frequency-doubling mode, but the operating frequency and natural mode frequencies did not correspond with one another. To further explicate experimental and simulation results, a two-degrees-of-freedom vibration model was developed. It was evident that the contact relationship between the dentate disc and scraper introduced strong nonlinear factors into the system, accounting for the frequency-doubling phenomenon and the difference between the BUE’s operating and mode frequencies. The BUE could be expected to facilitate the production of high-power micro-ultrasonic generators and has potential application value in the fields of mechanical processing, industrial production, and medical health.

在自然界中,切叶蚁通过相互刮动锉刀器官引起振动。在这项研究中,我们设计了一种模仿切叶蚁的仿生超声激振器(BUE)。通过实验测试和有限元模拟研究了 BUE 的工作特性。结果表明,BUE 可以产生稳定的超声波振动,而且激励频率只需输出频率(OF)的一半。这种倍频现象有利于实现 BUE 的微型化。为了进一步探索振动输出倍频现象,本研究设计了五种不同尺寸的刮刀,进行了激励和一阶固有频率测量试验,并进行了相应的有限元模拟。结果发现,每种铲运机都能以倍频模式工作,但工作频率和固有模态频率并不一致。为了进一步解释实验和模拟结果,建立了一个二自由度振动模型。结果表明,齿盘和刮刀之间的接触关系给系统带来了很强的非线性因素,从而导致了倍频现象以及 BUE 工作频率和模态频率之间的差异。该 BUE 可望促进大功率微型超声波发生器的生产,在机械加工、工业生产和医疗卫生领域具有潜在的应用价值。
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引用次数: 0
Design and Experimental Verification of a Roll Control Strategy for Large Wingspan Flapping-Wing Aerial Vehicle 大翼展拍翼飞行器滚转控制策略的设计与实验验证
IF 4.9 3区 计算机科学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2024-05-14 DOI: 10.1007/s42235-024-00532-4
Rui Meng, Bifeng Song, Jianlin Xuan, Xiaojun Yang, Dong Xue

Most flapping-wing aircraft wings use a single degree of freedom to generate lift and thrust by flapping up and down, while relying on the tail control surfaces to manage attitude. However, these aircraft have certain limitations, such as poor accuracy in attitude control and inadequate roll control capabilities. This paper presents a design for an active torsional mechanism at the wing's trailing edge, which enables differential variations in the pitch angle of the left and right wings during flapping. This simple mechanical form significantly enhances the aircraft's roll control capacity. The experimental verification of this mechanism was conducted in a wind tunnel using the RoboEagle flapping-wing aerial vehicle that we developed. The study investigated the effects of the control strategy on lift, thrust, and roll moment during flapping flight. Additionally, the impact of roll control on roll moment was examined under various wind speeds, flapping frequencies, angles of attack, and wing flexibility. Furthermore, several rolling maneuver flight tests were performed to evaluate the agility of RoboEagle, utilizing both the elevon control strategy and the new roll control strategy. The results demonstrated that the new roll control strategy effectively enhances the roll control capability, thereby improving the attitude control capabilities of the flapping-wing aircraft in complex wind field environments. This conclusion is supported by a comparison of the control time, maximum roll angle, average roll angular velocity, and other relevant parameters between the two control strategies under identical roll control input.

大多数拍翼飞机的机翼使用单自由度,通过上下拍动产生升力和推力,同时依靠尾部控制面来控制姿态。然而,这些飞机存在一定的局限性,例如姿态控制精度差,滚转控制能力不足。本文介绍了一种机翼后缘主动扭转机构的设计,它可以在拍打过程中实现左右机翼俯仰角的不同变化。这种简单的机械形式大大增强了飞机的滚转控制能力。我们利用自己研制的 RoboEagle 拍翼飞行器在风洞中对这一机制进行了实验验证。研究调查了拍翼飞行过程中控制策略对升力、推力和滚转力矩的影响。此外,还考察了在不同风速、拍打频率、攻角和机翼柔韧性条件下,滚转控制对滚转力矩的影响。此外,还利用升降舵控制策略和新的滚转控制策略进行了几次滚转机动飞行测试,以评估 RoboEagle 的灵活性。结果表明,新的滚转控制策略有效地增强了滚转控制能力,从而提高了拍翼飞行器在复杂风场环境中的姿态控制能力。通过比较两种控制策略在相同滚转控制输入下的控制时间、最大滚转角度、平均滚转角速度和其他相关参数,这一结论得到了支持。
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引用次数: 0
Enhanced Chimp Optimization Algorithm Using Attack Defense Strategy and Golden Update Mechanism for Robust COVID-19 Medical Image Segmentation 使用攻击防御策略和黄金更新机制的增强型 Chimp 优化算法,用于鲁棒 COVID-19 医学图像分割
IF 4.9 3区 计算机科学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2024-05-14 DOI: 10.1007/s42235-024-00539-x
Amir Hamza, Morad Grimes, Abdelkrim Boukabou, Samira Dib

Medical image segmentation is a powerful and evolving technology in medical diagnosis. In fact, it has been identified as a very effective tool to support and accompany doctors in their fight against the spread of the coronavirus (COVID-19). Various techniques have been utilized for COVID-19 image segmentation, including Multilevel Thresholding (MLT)-based meta-heuristics, which are considered crucial in addressing this issue. However, despite their importance, meta-heuristics have significant limitations. Specifically, the imbalance between exploration and exploitation, as well as premature convergence, can cause the optimization process to become stuck in local optima, resulting in unsatisfactory segmentation results. In this paper, an enhanced War Strategy Chimp Optimization Algorithm (WSChOA) is proposed to address MLT problems. Two strategies are incorporated into the traditional Chimp Optimization Algorithm. Golden update mechanism that provides diversity in the population. Additionally, the attack and defense strategies are incorporated to improve the search space leading to avoiding local optima. The experimental results were conducted by comparing WSChoA with recent and well-known algorithms using various evaluation metrics such as Feature Similarity Index (FSIM), Structural Similarity Index (SSIM), Peak signal-to-Noise Ratio (PSNR), Standard deviation (STD), Freidman Test (FT), and Wilcoxon Sign Rank Test (WSRT). The results obtained by WSChoA surpassed those of other optimization techniques in terms of robustness and accuracy, indicating that it is a powerful tool for image segmentation.

医学影像分割是医学诊断中一项功能强大且不断发展的技术。事实上,它已被确认为一种非常有效的工具,可以支持和配合医生对抗冠状病毒(COVID-19)的传播。COVID-19 图像分割采用了多种技术,包括基于多级阈值法(MLT)的元启发式技术,这些技术被认为是解决这一问题的关键。然而,尽管元启发式方法很重要,但也有很大的局限性。具体来说,探索和利用之间的不平衡以及过早收敛会导致优化过程陷入局部最优状态,从而导致不尽人意的分割结果。本文提出了一种增强型战争策略 Chimp 优化算法(WSChOA),以解决 MLT 问题。在传统的 Chimp 优化算法中加入了两种策略。黄金更新机制提供了种群的多样性。此外,还加入了攻击和防御策略,以改善搜索空间,从而避免局部最优。实验结果通过使用各种评估指标,如特征相似性指数(FSIM)、结构相似性指数(SSIM)、峰值信噪比(PSNR)、标准偏差(STD)、弗里德曼检验(FT)和威尔科克森符号等级检验(WSRT),将 WSChoA 与最新的知名算法进行比较。在鲁棒性和准确性方面,WSChoA 所获得的结果超过了其他优化技术,表明它是一种强大的图像分割工具。
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引用次数: 0
Elite Dung Beetle Optimization Algorithm for Multi-UAV Cooperative Search in Mountainous Environments 用于山区环境中多无人机合作搜索的精英蜣螂优化算法
IF 4.9 3区 计算机科学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2024-05-14 DOI: 10.1007/s42235-024-00528-0
Xiaoyong Zhang, Wei Yue

This paper aims to address the problem of multi-UAV cooperative search for multiple targets in a mountainous environment, considering the constraints of UAV dynamics and prior environmental information. Firstly, using the target probability distribution map, two strategies of information fusion and information diffusion are employed to solve the problem of environmental information inconsistency caused by different UAVs searching different areas, thereby improving the coordination of UAV groups. Secondly, the task region is decomposed into several high-value sub-regions by using data clustering method. Based on this, a hierarchical search strategy is proposed, which allows precise or rough search in different probability areas by adjusting the altitude of the aircraft, thereby improving the search efficiency. Third, the Elite Dung Beetle Optimization Algorithm (EDBOA) is proposed based on bionics by accurately simulating the social behavior of dung beetles to plan paths that satisfy the UAV dynamics constraints and adapt to the mountainous terrain, where the mountain is considered as an obstacle to be avoided. Finally, the objective function for path optimization is formulated by considering factors such as coverage within the task region, smoothness of the search path, and path length. The effectiveness and superiority of the proposed schemes are verified by the simulation.

本文旨在解决山地环境下多无人机协同搜索多目标的问题,考虑了无人机动力学和先验环境信息的约束。首先,利用目标概率分布图,采用信息融合和信息扩散两种策略,解决不同无人机搜索不同区域导致的环境信息不一致问题,从而提高无人机群的协同性。其次,利用数据聚类方法将任务区域分解为多个高价值子区域。在此基础上,提出分层搜索策略,通过调整飞行器的高度,对不同概率区域进行精确或粗略搜索,从而提高搜索效率。第三,提出了基于仿生学的精英蜣螂优化算法(EDBOA),通过精确模拟蜣螂的社会行为,规划出满足无人机动力学约束条件的路径,并适应山地地形,将山体视为需要避开的障碍物。最后,通过考虑任务区域内的覆盖范围、搜索路径的平滑度和路径长度等因素,制定了路径优化的目标函数。仿真验证了所提方案的有效性和优越性。
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引用次数: 0
Adaptive Control of Lower-Limb Exoskeletons for Walking Assistance Based on Inter-Joint Coordination 基于关节间协调的下肢外骨骼行走辅助自适应控制
IF 4.9 3区 计算机科学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2024-05-14 DOI: 10.1007/s42235-024-00537-z
Chaoyang Li, Lincong Luo, Zhi Liu, Tianchi Chen, Songxiang Liu, Ye He, Xiaoan Chen, Lei Li, Wei Tech Ang

Unilateral motor impairment can disrupt the coordination between the joints, impeding the patient’s normal gait. To assist such patients to walk normally and naturally, an adaptive control algorithm based on inter-joint coordination was proposed in this work for lower-limb exoskeletons. The control strategy can generate the reference trajectory of the affected leg in real time based on a motion coordination model between the joints, and adopt an adaptive controller with virtual windows to track the reference trajectory. Long Short-Term Memory (LSTM) network was also adopted to establish the coordination model between the joints of both lower limbs, which was optimized by preprocessing angle information and adding gait phase information. In the adaptive controller, the virtual windows were symmetrically distributed around the reference trajectory, and its width was adjusted according to the gait phase of the auxiliary leg. In addition, the impedance parameters of the controller were updated online to match the motion capacity of the affected leg based on the spatiotemporal symmetry factors between the bilateral gaits. The LSTM coordination model demonstrated good accuracy and generality in the gait database of seven individuals, with an average root mean square error of 3.5(^circ) and 4.1(^circ) for the hip and knee joint angle estimation, respectively. To further evaluate the control algorithm, four healthy subjects walked wearing the exoskeleton while additional weights were added around the ankle joint to simulate an asymmetric gait. From the experimental results, it was shown that the algorithm improved the gait symmetry of the subjects to a normal level while exhibiting great adaptability to different subjects.

单侧运动障碍会破坏关节间的协调,阻碍患者的正常步态。为了帮助这类患者正常、自然地行走,本文提出了一种基于关节间协调的下肢外骨骼自适应控制算法。该控制策略可根据关节间的运动协调模型实时生成患肢的参考轨迹,并采用带有虚拟窗口的自适应控制器来跟踪参考轨迹。此外,还采用了长短期记忆(LSTM)网络来建立双下肢关节间的协调模型,并通过预处理角度信息和添加步态相位信息对该模型进行了优化。在自适应控制器中,虚拟窗口围绕参考轨迹对称分布,其宽度根据辅助腿的步态相位进行调整。此外,控制器的阻抗参数也进行了在线更新,以根据双侧步态之间的时空对称因子匹配患肢的运动能力。在七个人的步态数据库中,LSTM 协调模型表现出了良好的准确性和通用性,髋关节和膝关节角度估计的平均均方根误差分别为 3.5(^circ)和 4.1(^circ)。为了进一步评估控制算法,四名健康受试者穿着外骨骼行走,同时在踝关节周围增加额外的重量,以模拟不对称步态。实验结果表明,该算法将受试者的步态对称性提高到了正常水平,同时对不同受试者表现出了很强的适应性。
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引用次数: 0
In Situ Reconfiguration of Assembling Pattern for Modular Continuum Robots 现场重新配置模块化连续机器人的装配模式
IF 4.9 3区 计算机科学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2024-05-14 DOI: 10.1007/s42235-024-00523-5
Jie Zhang, Jiannan Cai, Ke Ma, Jinzhao Yang, Zhigang Wu, Haijun Peng, Jianing Wu

Modular continuum robots possess significant versatility across various scenarios; however, conventional assembling methods typically rely on linear connection between modules. This limitation can impede the robotic interaction capabilities, especially in specific engineering applications. Herein, inspired by the assembling pattern between the femur and tibia in a human knee, we proposed a multidirectional assembling strategy. This strategy encompasses linear, oblique, and orthogonal connections, allowing a two-module continuum robot to undergo in-situ reconfiguration into three distinct initial configurations. To anticipate the final configuration resulting from diverse assembling patterns, we employed the positional formulation finite element framework to establish a mechanical model, and the theoretical results reveal that our customizable strategy can offer an effective route for robotic interactions. We showcased diverse assembling patterns for coping with interaction requirements. The experimental results indicate that our modular continuum robot not only reconfigures its initial profile in situ but also enables on-demand regulation of the final configuration. These capabilities provide a foundation for the future development of modular continuum robots, enabling them to be adaptable to diverse environments, particularly in unstructured surroundings.

模块化连续机器人在各种应用场景中都具有显著的多功能性;然而,传统的组装方法通常依赖于模块之间的线性连接。这种局限性会阻碍机器人的交互能力,尤其是在特定的工程应用中。在此,我们从人类膝关节中股骨和胫骨之间的组装模式中汲取灵感,提出了一种多向组装策略。该策略包括线性、斜向和正交连接,允许双模块连续机器人在原位重新配置为三种不同的初始配置。为了预测不同组装模式产生的最终配置,我们采用了位置配方有限元框架来建立机械模型,理论结果表明,我们的可定制策略可为机器人互动提供有效途径。我们展示了应对交互要求的多样化组装模式。实验结果表明,我们的模块化连续机器人不仅能在原地重新配置其初始轮廓,还能按需调节最终配置。这些功能为模块化连续机器人的未来发展奠定了基础,使其能够适应各种环境,尤其是非结构化环境。
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引用次数: 0
Improved Dwarf Mongoose Optimization Algorithm for Feature Selection: Application in Software Fault Prediction Datasets 用于特征选择的改进矮獴优化算法:在软件故障预测数据集中的应用
IF 4.9 3区 计算机科学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2024-05-14 DOI: 10.1007/s42235-024-00524-4
Abdelaziz I. Hammouri, Mohammed A. Awadallah, Malik Sh. Braik, Mohammed Azmi Al-Betar, Majdi Beseiso

Feature selection (FS) plays a crucial role in pre-processing machine learning datasets, as it eliminates redundant features to improve classification accuracy and reduce computational costs. This paper presents an enhanced approach to FS for software fault prediction, specifically by enhancing the binary dwarf mongoose optimization (BDMO) algorithm with a crossover mechanism and a modified positioning updating formula. The proposed approach, termed iBDMOcr, aims to fortify exploration capability, promote population diversity, and lastly improve the wrapper-based FS process for software fault prediction tasks. iBDMOcr gained superb performance compared to other well-esteemed optimization methods across 17 benchmark datasets. It ranked first in 11 out of 17 datasets in terms of average classification accuracy. Moreover, iBDMOcr outperformed other methods in terms of average fitness values and number of selected features across all datasets. The findings demonstrate the effectiveness of iBDMOcr in addressing FS problems in software fault prediction, leading to more accurate and efficient models.

特征选择(FS)在机器学习数据集的预处理中起着至关重要的作用,因为它可以消除冗余特征,从而提高分类准确性并降低计算成本。本文提出了一种用于软件故障预测的增强型特征选择方法,特别是通过交叉机制和修改后的定位更新公式来增强二元矮獴优化(BDMO)算法。所提出的方法被称为 iBDMOcr,旨在加强探索能力,促进种群多样性,最后改进软件故障预测任务中基于封装器的 FS 流程。与其他备受推崇的优化方法相比,iBDMOcr 在 17 个基准数据集中表现出色。在 17 个数据集中,iBDMOcr 在 11 个数据集中的平均分类准确率排名第一。此外,在所有数据集上,iBDMOcr 在平均适合度值和所选特征数量方面都优于其他方法。这些研究结果表明,iBDMOcr 在解决软件故障预测中的 FS 问题方面非常有效,可以建立更准确、更高效的模型。
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引用次数: 0
Electrospun Aloe Vera Extract Loaded Polycaprolactone Scaffold for Biomedical Applications: A Promising Candidate for Corneal Stromal Regeneration 用于生物医学应用的电纺芦荟提取物负载聚己内酯支架:角膜基质再生的理想候选材料
IF 4.9 3区 计算机科学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2024-05-13 DOI: 10.1007/s42235-024-00520-8
Amin Orash Mahmoud Salehi, Mohammad Rafienia, Narsimha Mamidi, Saeed Heidari Keshel, Alireza Baradaran-Rafii

Corneal diseases, the second leading cause of global vision loss affecting over 10.5 million people, underscores the unmet demand for corneal tissue replacements. Given the scarcity of fresh donor corneas and the associated risks of immune rejection, corneal tissue engineering becomes imperative. Developing nanofibrous scaffolds that mimic the natural corneal structure is crucial for creating transparent and mechanically robust corneal equivalents in tissue engineering. Herein, Aloe Vera Extract (AVE)/Polycaprolactone (PCL) nanofibrous scaffolds were primed using electrospinning. The electrospun AVE/PCL fibers exhibit a smooth, bead-free morphology with a mean diameter of approximately 340 ± 95 nm and appropriate light transparency. Mechanical measurements reveal Young’s modulus and ultimate tensile strength values of around 3.34 MPa and 4.58 MPa, respectively, within the range of stromal tissue. In addition, cell viability of AVE/PCL fibers was measured against Human Stromal Keratocyte Cells (HSKCs), and improved cell viability was observed. The cell-fiber interactions were investigated using scanning electron microscopy. In conclusion, the incorporation of Aloe Vera Extract enhances the mechanical, optical, hydrophilic, and biological properties of PCL fibers, positioning PCL/AVE fiber scaffolds as promising candidates for corneal stromal regeneration.

角膜疾病是导致全球超过 1050 万人视力丧失的第二大原因,这凸显出角膜组织替代品的需求尚未得到满足。鉴于新鲜供体角膜的稀缺性和相关的免疫排斥风险,角膜组织工程变得势在必行。开发模仿天然角膜结构的纳米纤维支架对于在组织工程中创建透明且机械坚固的角膜等效物至关重要。在此,我们利用电纺丝技术为芦荟提取物(AVE)/聚己内酯(PCL)纳米纤维支架打底。电纺的 AVE/PCL 纤维形态光滑无珠,平均直径约为 340 ± 95 nm,具有适当的透光性。力学测量显示,杨氏模量和极限拉伸强度值分别约为 3.34 兆帕和 4.58 兆帕,在基质组织范围内。此外,还针对人类基质角膜细胞(HSKCs)测量了 AVE/PCL 纤维的细胞存活率,观察到细胞存活率有所提高。使用扫描电子显微镜研究了细胞与纤维之间的相互作用。总之,芦荟提取物的加入增强了 PCL 纤维的机械、光学、亲水和生物特性,使 PCL/AVE 纤维支架成为角膜基质再生的理想候选材料。
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引用次数: 0
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