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Importance of Body Condition Scoring in Reproductive Performance of Dairy Cows: A Review 体况评分在奶牛繁殖性能中的重要性
Pub Date : 2021-07-16 DOI: 10.4236/ojvm.2021.117018
Sayed Ahmad Nazhat, Amanullah Aziz, Jahid Zabuli, Shahpoor Rahmati
Body condition score (BCS) is a subjective method of assessing the amount of metabolizable energy stored in fat and muscle (body reserves) on a live animal. Body condition scores provide an indication of the energy status of dairy cattle. It can be used on both heifers and cows, although primarily they are used on the lactating dairy herd. Adjusting the nutritional program to obtain desired body condition at different stages of production is necessary to enhance production efficiency. Females that are too thin or too fat can be an expensive investment. Nutritional management is the most crucial one and is a key factor for normal production and reproduction cycle. Thus, it could be used as a management and selection tool to improve reproductive performance in dairy cows.
体况评分(Body condition score, BCS)是一种评估活体动物脂肪和肌肉中代谢能量(身体储备)的主观方法。体况评分是奶牛能量状态的一个指标。它可以用于小母牛和奶牛,尽管它们主要用于哺乳奶牛群。在不同的生产阶段调整营养计划以获得所需的身体状态是提高生产效率的必要条件。太瘦或太胖的雌性都是一笔昂贵的投资。营养管理是最关键的,是维持正常生产和繁殖周期的关键因素。因此,它可以作为提高奶牛繁殖性能的管理和选择工具。
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引用次数: 10
Effort and Recovery in Nellore Oxen during Vaquejada Assessed with Ocular and Tail Infrared Thermography Superficial Temperature 用眼和尾红外热像仪评价内洛尔牛在Vaquejada期间的努力和恢复
Pub Date : 2021-07-16 DOI: 10.4236/ojvm.2021.117017
R. Cruz, César Fabiano Vilela, Diego Barbosa de Freitas, Calena Costa Paixão, C. J. F. L. Silva, K. Trindade, Juliette P. da Silva, H. Manso, H. C. M. Filho
Infrared thermography (IRT) has emerged for evaluation of animal welfare. To test the hypothesis that cattle subjected to vaquejada increased temperature in the eye (CLO) and tail due to physical effort, a research was developed that aimed to measure maximum surface temperature in the CLO and base of tail using IRT. Eighty Nellore cattle were used, which were subjected to physical effort in three periods (morning, afternoon and night). IRT was performed at CLO and base of tail, in rest pen/corral (control) and during the vaquejada (pre- and post-run). Tails’ analysis was divided in 3 points (E1, E2 and E3). ANOVA and Tukey’s test (p < 0.001) were used for analysis. Temperatures were higher in morning and afternoon and different from those at night on control (p < 0.001). During vaquejada with one run, CLO was higher in pre-run, followed by a slight reduction in post-run (p < 0.001). Analyzing temperatures variations at tail, higher temperatures were observed in the morning, followed by the evening and night (p < 0.001), but without differences within the pre- and post-run periods. Temperatures at tail’s points were higher in morning period in cattle with two runs (p < 0.001). Finally, it was concluded that there were no increases in CLO or at tail’s points after vaquejada races. The IRT method was efficient in determining surface temperatures in CLO and tail points in cattle under the same conditions and may be a good noninvasive method for clinical and welfare assessments.
红外热像仪(IRT)是一种用于动物福利评价的技术。为了验证牛在接受vaquejada训练时,由于身体的努力而导致眼和尾温度升高的假设,我们开展了一项研究,旨在利用IRT测量眼和尾底部的最高表面温度。使用了80头内洛尔牛,它们在三个时间段(上午、下午和晚上)受到体力劳动。IRT分别在CLO和尾部、休息栏/畜栏(对照组)和vaquejada期间(跑前和跑后)进行。尾分析分为3个点(E1, E2和E3)。采用方差分析和Tukey检验(p < 0.001)进行分析。对照组上午和下午温度较高,与夜间不同(p < 0.001)。在vaquejada进行一次跑步时,CLO在跑步前较高,随后在跑步后略有降低(p < 0.001)。分析尾部温度变化,早晨温度较高,其次是傍晚和夜间(p < 0.001),但在跑步前后没有差异。两组牛尾点温度在早晨较高(p < 0.001)。最后,我们得出结论,在vaquejada比赛后,CLO和尾点没有增加。在相同条件下,IRT方法可以有效地测定牛的CLO和尾部点的表面温度,可能是一种良好的无创临床和福利评估方法。
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引用次数: 1
Assessment of a Novel Surgical Technique for Acoustic Transmitter Insertion 一种新的声学发射器插入手术技术的评估
Pub Date : 2021-07-16 DOI: 10.4236/ojvm.2021.117016
Ashley Kelican, Nathan Huysman, Lauren Anne Van Rysselberge, J. Voorhees, M. E. Barnes
This study compared three surgical techniques to implant acoustic transmitters in rainbow trout (Oncorhynchus mykiss): 1) acoustic transmitter insertion via a ventral incision closed with two sutures, 2) insertion via a ventral incision with no sutures, 3) insertion via a lateral incision with no sutures. A control group consisting of fish that were only anesthetized and handled was also included. Tag retention, wound healing, inflammation, growth, and survival were recorded over a 12-week period. Tag retention was significantly different among the treatments, at 100% in the unsutured ventral incision group, 89% in the lateral incision group, and 63% in the sutured ventral incision group. Surgical wounds in the unsutured treatment groups showed significantly less inflammation than the sutured treatment. Wound closure occurred significantly faster in the ventral incision groups compared to the lateral incision group. The results of this study show the utility of using unsutured surgical methods for implanting acoustic transmitters in rainbow trout.
本研究比较了在虹鳟鱼(Oncorhynchus mykiss)中植入声学发射器的三种手术技术:1)声学发射器通过两道缝合的腹侧切口置入,2)不缝合的腹侧切口置入,3)不缝合的外侧切口置入。另外还包括一组只经过麻醉和处理的鱼。在12周的时间内记录标签保留、伤口愈合、炎症、生长和生存。不同治疗组标签保留率差异显著,未缝合腹侧切口组为100%,外侧切口组为89%,缝合腹侧切口组为63%。手术创面未缝合组的炎症反应明显少于缝合组。腹侧切口组伤口愈合明显快于外侧切口组。本研究的结果显示了使用非缝合手术方法在虹鳟鱼体内植入声学发射器的效用。
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引用次数: 2
Determination of Hemobiochemical Profiles of Apparently Healthy Exotic Breed of Dogs in Jos, Plateau State, Nigeria 尼日利亚高原州乔斯市明显健康的外来犬种血液生化图谱的测定
Pub Date : 2021-06-02 DOI: 10.4236/OJVM.2021.116015
K. Ogbu, K. Ezema, Ijeoma C. Adieme, R. I. Malgwi, Jibreel A. Sabo, Philip N. Ayuba, Samuel A. Nguety, Simone T. Atuna, Francis O. Emeribe, J. Shallmizhili, B. Anene
The hematological and serum biochemical profiles of apparently healthy exotic breed of dogs in Jos, Plateau state, Nigeria was studied in this research. One hundred and seven exotic breed of dogs were examined from October - December, 2018. The results from physical, clinical examinations and parasitological examinations were used to determine apparently healthy exotic breed of dogs which were used in this study. The parameters measured were rectal temperature, parasitaemia, hematology which included red blood cells count, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin and hemoglobin content; total white blood cell count, including eosinophils, neutrophils, lymphocytes, monocytes, granulocytes counts, and platelets; serum biochemical parameters also included Aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total proteins, albumin, globulin, blood urea nitrogen, creatinine, cholesterol and triglyceride, using standard methods. Data generated from the study were statistically analyzed using students t-test one-way analysis of variance while the level of significance was measured at p < 0.05. From this study, hematological and serum biochemical values were comparable to and not different from those reported for dogs in available literature, but the upper and lower reference limits (minimum and maximum values) were different for most parameters. Sex-related significant difference (p < 0.05) occurred only in WBC and total protein while age-related significant difference occurred only in total protein and urea level. Breed affected the MCHC and total bilirubin level which was evident in higher MCHC level in Neapolitan mastiff than Alsatian breed while total bilirubin was higher in Alsatian breed than Bull mastiff (p < 0.05). The present study has presented preliminary information on the range of hematological and serum biochemistry profile of exotic breed of dogs in Jos, Plateau state, Nigeria which may be useful to biomedical researchers and veterinary clinicians.
本研究对尼日利亚高原州乔斯地区明显健康的外来犬种的血液学和血清生化特征进行了研究。从2018年10月至12月,对107只外来品种的狗进行了检查。采用体格检查、临床检查和寄生虫学检查的结果来确定本研究所用的外来犬种表面健康。测量的参数包括直肠温度、寄生虫血症、血液学指标,包括红细胞计数、红细胞比容、平均红细胞体积、平均红细胞血红蛋白、平均红细胞血红蛋白和血红蛋白含量;白细胞总数,包括嗜酸性粒细胞、中性粒细胞、淋巴细胞、单核细胞、粒细胞计数和血小板计数;血清生化指标:天门冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶、总蛋白、白蛋白、球蛋白、血尿素氮、肌酐、胆固醇、甘油三酯,采用标准方法测定。本研究产生的数据采用学生t检验单因素方差分析进行统计学分析,p < 0.05为显著性水平。从本研究中,血液学和血清生化值与现有文献中报道的狗的血液学和血清生化值相当,但大多数参数的上下限(最小值和最大值)不同。白细胞和总蛋白存在性别相关差异(p < 0.05),总蛋白和尿素存在年龄相关差异(p < 0.05)。品种影响MCHC和总胆红素水平,其中那不勒斯獒的MCHC水平高于阿尔萨斯獒,阿尔萨斯獒的总胆红素水平高于公牛獒(p < 0.05)。本研究提供了尼日利亚乔斯高原州外来犬种血液学和血清生化特征范围的初步信息,可能对生物医学研究人员和兽医临床医生有用。
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引用次数: 0
Selected Aspects of Camel Immune System and Immune Responses 骆驼免疫系统和免疫反应的选择
Pub Date : 2021-06-02 DOI: 10.4236/ojvm.2021.116013
Saeed Y. Al Ramadan, K. T. A. Salem, I. Alshubaith, A. Al-Ali, S. Abohelaika, M. Moqbel, A. Alluwaimi
The camel economy is of considerable importance for arid countries. In the last decade, studies about camel immune system and immune responses have recorded increasing interest. However, drawing a comprehensive picture of the camel immune system remains far from reached. A major part of this review is to cover the studies of the primary and secondary immune organs and the markers of the camel immune cells and certain lymphoid tissues. At the same time, immune responses to different diseases and the nature of effective immunity were included, with an emphasis on the most important zoonotic diseases in camels such as MERS CoV; brucellosis. New findings on the diversity mechanisms of camel immunoglobulin genes were addressed. However, detail of the mechanism of MHC-restricted cellular immunity and the mechanism of B lymphocyte activation in camels await further attention. Interestingly, the gross and the histological structure of the lymphoid tissues of the camel’s thymus, tonsils, and peyer’s patches have indicated significant differences from other animals in terms of structure and function. The most peculiar CD expression, such as LPAM-I, MAdCAM-1 and CX3CR1, in certain camel cells and tissues refers to possible extraordinary mechanisms of immune hemostasis in camel in comparison to other ruminants. The widely applied immunodiagnostic techniques to control camel diseases and to assist in improving the camel resistance were considered. Extensive studies of the camel immune system were greatly hampered by lack of specific reagents to camel markers and low funds in the field of camel immunology.
骆驼经济对干旱国家具有相当重要的意义。在过去的十年里,人们对骆驼免疫系统和免疫反应的研究越来越感兴趣。然而,要全面了解骆驼的免疫系统还远远不够。这篇综述的主要部分是涵盖初级和次级免疫器官以及骆驼免疫细胞和某些淋巴组织的标志物的研究。同时,还包括对不同疾病的免疫反应和有效免疫的性质,重点是骆驼最重要的人畜共患疾病,如MERS-CoV;布鲁氏菌病。对骆驼免疫球蛋白基因多样性机制的新发现进行了阐述。然而,MHC限制细胞免疫的机制和骆驼B淋巴细胞活化的机制的细节有待进一步关注。有趣的是,骆驼胸腺、扁桃体和派耶斑的淋巴组织的大体和组织学结构表明,在结构和功能方面与其他动物存在显著差异。与其他反刍动物相比,在某些骆驼细胞和组织中最特殊的CD表达,如LPAM-I、MAdCAM-1和CX3CR1,是指骆驼免疫止血的可能特殊机制。考虑了广泛应用的免疫诊断技术来控制骆驼疾病和帮助提高骆驼抵抗力。由于缺乏针对骆驼标志物的特异性试剂以及骆驼免疫学领域的资金不足,对骆驼免疫系统的广泛研究受到了极大阻碍。
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引用次数: 2
Determination of mRNA Expression of Typical Proteins of Tight Junctions in the Intestinal Mucosa of Broilers (Gallus gallus domesticus) 肉仔鸡肠黏膜紧密连接典型蛋白mRNA表达量的测定
Pub Date : 2021-06-02 DOI: 10.4236/ojvm.2021.116014
E. A. Garcia, Carlos Ignacio Soto Zárate, Francisco Rodolfo González Díaz, J. Bordes, G. Fariña, C. Tovar
Tight junctions are mainly formed by two types of proteins; claudins and occludin, both of which are fundamental to maintain the integrity and barrier function of the intestinal epithelium. This barrier function allows for the absorption of nutrients, mainly by transcytosis; however, in birds, 90% of the substances are absorbed by paracellular mechanisms. Despite this, claudins present in the different parts of the intestinal tract of adult chickens are not known, much less their functional role. This study aimed to determine the presence of mRNA of claudins 1, 2, 3, 5, 10, 12, 16 and occludin, in the different regions of the intestine (duodenum, jejunum, ileum, cecum, and rectum) in chickens (Gallus gallus domesticus) through RT-PCR. To meet this goal, 7 weeks old roosters destined for slaughter and chicken embryos of 16 days of incubation (positive control) were used. For all the processed samples, amplicons of the expected size were obtained; claudin 1 (662 pb), claudin 2 (162 pb), claudin 3 (185 pb), claudin 5 (224 pb), claudin 10 (687 pb), claudin 12 (738 pb), claudin 16 (191 pb) and occludin (430 pb). To corroborate these findings, obtained amplicons were sequenced and, subsequently, a basic alignment was performed on the NCBI, obtaining a correlation of 100% with the original sequences in all analyzed samples. To our knowledge, the present work represents the first written report regarding the presence of mRNA of the main proteins involved in tight junction formation throughout the intestinal tract of domestic chickens of 7 weeks of age. These findings will allow elucidating the specific function of each of the reported proteins in the process of paracellular absorption in chickens.
紧密连接主要由两种类型的蛋白质形成;claudins和occludin,这两种物质都是维持肠上皮完整性和屏障功能的基础。这种屏障功能允许营养物质的吸收,主要是通过胞吞作用;然而,在鸟类中,90%的物质被细胞旁机制吸收。尽管如此,在成年鸡肠道的不同部位存在的claudins尚不清楚,更不用说它们的功能作用了。本研究旨在通过RT-PCR测定鸡(Gallus Gallus domesticus)肠道不同区域(十二指肠、空肠、回肠、盲肠和直肠)中claudins 1、2、3、5、10、12、16和occludin的mRNA的存在。为了实现这一目标,使用了准备屠宰的7周龄公鸡和孵化16天的鸡胚(阳性对照)。对于所有处理过的样品,获得预期大小的扩增子;claudin 1(662 pb)、claudin 2(162 pb)、claudin 3(185 pb)、cliudin 5(224 pb)、cloudin 10(687 pb)、克劳迪12(738 pb),claudin 16(191 pb)和occludin(430 pb)。为了证实这些发现,对获得的扩增子进行测序,随后对NCBI进行基本比对,在所有分析样本中获得与原始序列100%的相关性。据我们所知,本工作代表了关于在7周龄家鸡的整个肠道中存在与紧密连接形成有关的主要蛋白质的mRNA的第一份书面报告。这些发现将有助于阐明每种报道的蛋白质在鸡细胞旁吸收过程中的特定功能。
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引用次数: 0
Intestinal Protothecosis in a Young Bengal Cat 幼年孟加拉猫的肠道原膜细胞病
Pub Date : 2021-04-30 DOI: 10.4236/OJVM.2021.115011
S. Manfredini, L. Formaggini, M. Marino, L. Venco
Background: Intestinal protothecosis is an uncommon and insidious mycotic disease. Only one human case and a few rare cases in dogs have been reported. To the authors’ knowledge, intestinal protothecosis has never been reported in cats. Case description: This paper describes a case of intestinal protothecosis in a nine-month-old male, Bengal cat. The cat presented because of onset of haemorrhagic diarrhoea. Investigations allowed diagnosis of intestinal protothecosis, confirmed by PCR test on faeces. Treatment with itraconazole did not improve the clinical signs. Treatment with nystatin was prescribed and caused improvement in the clinical signs and decreased number of pathogens seen on faecal cytology. PCR on faecal samples was negative two months after treatment, with complete resolution of symptoms. Conclusion: Infection with Prototheca should be part of the list of differential diagnoses for diarrhoea in cats. nystatin was effective in treating the infection in this case; this drug should be considered as a first-line treatment in cats as well as in dogs, in which protothecosis appears to have a poor prognosis. Although protothecosis is not considered a zoonotic disease, cases of algal infections in companion animals might be considered indicators of environmental risks for humans.
背景:肠道原卵泡膜细胞病是一种罕见且隐蔽的真菌性疾病。只报告了一例人类病例和少数罕见的狗病例。据作者所知,从未有猫肠道原膜细胞病的报道。病例描述:本文描述了一例9个月大的孟加拉猫肠道原卵泡膜细胞病。这只猫是由于出血性腹泻而出现的。通过对粪便进行聚合酶链式反应检测,可以诊断为肠道原泡细胞病。伊曲康唑治疗并没有改善临床症状。处方使用制霉菌素治疗,改善了临床症状,减少了粪便细胞学检查中的病原体数量。治疗两个月后,粪便样本的聚合酶链式反应呈阴性,症状完全缓解。结论:原鞘感染应作为猫腹泻鉴别诊断的一部分。制霉菌素治疗本例感染效果良好;这种药物应该被认为是猫和狗的一线治疗方法,因为原卵泡膜细胞病的预后较差。尽管原卵泡膜细胞病不被认为是一种人畜共患疾病,但伴侣动物中的藻类感染病例可能被认为是人类环境风险的指标。
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引用次数: 0
A Static Bioimpedance and Dynamic Acoustic Myography Preliminary Assessment of Low Frequency Therapeutic Ultrasound Treatment of the Shoulder Muscle Trapezius: An Equine Study 低频超声治疗马斜方肌的静态生物阻抗和动态声学肌图初步评估
Pub Date : 2021-04-01 DOI: 10.4236/OJVM.2021.114008
Diane lsbell, Sebastian Laguna, A. Harrison
Background: In horses, therapeutic ultrasound has been shown to be beneficial for suspensory ligament injuries, and more generally to stimulate tissue repair and reduce localized pain. However, it has yet to be examined in relation to soft tissue injuries sustained during competition or in connection with rider/saddle interactions. Aim: The aim of the present study was to examine the efficacy of topical low-frequency therapeutic ultrasound on the shoulder muscles of injured horses. Methods: Muscle mass and cellular health was assessed for M. trapezius using non-invasive multi-frequency bioimpedance, and dynamic improvements in M. trapezius and M. latissimus dorsi were examined using acoustic myography (AMG). A total of 8 injured horses (painful to palpation and atrophied) were treated using an EQ Pro Therapy unit, following a set protocol that comprised treatment every other day for 10 treatments. Pre-treatment values were then compared with post-treatment measurements (1 & 6 months later). Results: The mfBIA results for M. trapezius showed a significant improvement (P 0.05) of EQ Pro Therapy treatment for the membrane capacitance (Mc) and phase angle (PA) parameters when comparing the Pre vs Post 1 and Post 1 vs Post 2 measurements. Similar improvements were seen for the AMG measurements for M. trapezius and M. latissimus dorsi where statistically significant improvements in the muscle efficiency (E-score) and temporal summation (T-score) as well as the spatial summation (S-score) and mean of the ST-scores were noted (P 0.05 to P 0.01). Conclusion: The present findings suggest that EQ Pro Therapy treatment is efficacious in terms of equine cases involving soft tissue shoulder muscle injuries (painful to palpation and atrophied). The main improvements noted were not only increased muscle mass and cellular health but also improved force production, better coordination, and lateral balance, which persisted for at least six months after treatment.
背景:在马身上,治疗性超声已被证明对悬吊韧带损伤有益,更普遍地说,对刺激组织修复和减轻局部疼痛有益。然而,它与比赛中遭受的软组织损伤或与骑手/马鞍的相互作用有关,尚待检查。目的:本研究的目的是检验局部低频治疗超声对受伤马肩部肌肉的疗效。方法:使用非侵入性多频生物阻抗评估斜方肌的肌肉质量和细胞健康,并使用声学肌电图(AMG)检查斜方肌和背阔肌的动态改善。共有8匹受伤的马(触诊疼痛和萎缩)使用EQ Pro治疗装置进行治疗,遵循一套方案,包括每隔一天治疗10次。然后将治疗前的值与治疗后的测量值进行比较(1和6个月后)。结果:斜方肌的mfBIA结果显示,当比较Pre与Post 1以及Post 1与Post 2的测量时,EQ Pro Therapy治疗在膜电容(Mc)和相位角(PA)参数方面有显著改善(P 0.05)。斜方肌和背阔肌的AMG测量也有类似的改善,其中肌肉效率(E评分)、时间总和(T评分)、空间总和(S评分)和ST评分平均值均有统计学显著改善(P 0.05至P 0.01)马的术语涉及软组织肩部肌肉损伤(触诊疼痛和萎缩)。注意到的主要改善不仅是肌肉质量和细胞健康的增加,而且还改善了力量的产生、更好的协调和横向平衡,这在治疗后至少持续了六个月。
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引用次数: 1
Comparison of Two Enzyme Immunoassays and Four Lysate Antigens for the Detection of Antibody in Canine Blastomycosis 两种酶免疫法和四种裂解抗原检测犬胚菌病抗体的比较
Pub Date : 2021-04-01 DOI: 10.4236/OJVM.2021.114009
M. Day, Arin C Esterbrook, Ignatius Bisharat, Abdullah Saleh Albqomi, Bryn C. Kennell, Russ Manteca, Heaton Oakes, Geoffrey M. Scalarone, G. Scalarone
Blastomycosis, the systemic fungal disease of humans and animals caused by Blastomyces dermatitidis and the cryptic species Blastomyces gilchristii, is often misdiagnosed as a bacterial or viral pulmonary disease. Therefore, the development of improved immunodiagnostic assays for this disease has been the primary focus of research in our laboratory. The present study was designed to evaluate four Blastomyces yeast-phase lysate antigenic preparations (human, 597, Eagle River, WI; dog, ERC-2, WI; Human, B5927, Mountain Iron, MN; soil, 85, Georgia, ATCC 56920) for their ability to detect antibody in 48 serum specimens from dogs with diagnosed blastomycosis using an indirect ELISA (STD) compared to a biotin-streptavidin ELISA (B-SA). All four lysate antigens were able to detect antibodies in the specimens with mean absorbance values ranging from 0.930 (B5927) to 1.142 (ERC-2) with the STD ELSA and from 1.395 (B5927) to 1.775 (85) with the B-SA ELISA. The results indicated that both ELISA methods could be utilized for antibody detection, but the B-SA ELISA exhibited greater sensitivity than the STD ELISA with all four of the lysates.
芽生菌病是由皮氏芽生菌和隐种吉尔克里斯蒂芽生菌引起的人类和动物的系统性真菌病,经常被误诊为细菌或病毒性肺部疾病。因此,开发改进的免疫诊断方法一直是我们实验室的主要研究重点。本研究旨在评估四种芽孢酵母相裂解物抗原制剂(人,597,威斯康星州鹰河;狗,ERC-2,威斯康星州;人,B5927,明尼苏达州Mountain Iron;土壤,85,乔治亚州,ATCC 56920)使用间接ELISA(STD)与生物素-链霉亲和素ELISA(B-SA)相比,在48份诊断为芽孢菌病的狗血清样本中检测抗体的能力。所有四种裂解物抗原都能够用STD ELSA检测样本中的抗体,其平均吸光度值范围为0.930(B5927)至1.142(ERC-2),用B-SA ELISA检测样本中抗体的平均吸光度值为1.395(B592七)至1.775(85)。结果表明,两种ELISA方法都可以用于抗体检测,但B-SA ELISA对所有四种裂解物的敏感性都高于STD ELISA。
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引用次数: 0
Clinical Features of Post-Parturient Hemoglobinuria in Dairy Cattle and Buffaloes: A Review 奶牛和水牛产后血红蛋白尿的临床特征
Pub Date : 2021-04-01 DOI: 10.4236/OJVM.2021.114010
Shahpoor Rahmati, Amanullah Aziz, M. Tawfeeq, Jahed Zabuli, Sayed Ahmad Nazhat
Postpartum hemoglobinuria (PPH) is a sporadic noninfectious syndrome which normally seen in bovine around the world. PPH is most commonly affecting high-productive cows and buffaloes during the early pregnancy and early lactation period. PPH stands as a serious threat to the dairy cattle and buffaloes in Afghanistan, Pakistan, and India, affecting a considerable number of animals every year. It is characterized by hemoglobinuria, intravenous hemolysis, severe anemia, and death due to anemia and hypoxia. However, the exact mechanism of the mentioned illness is not completely understood. Lots of comprehensive studies have been done and/or still are in progress in order to find the exact causes of intravascular hemolysis that is responsible for hemoglobinuria in the mentioned disease. But, hypophosphatemia in the high productive milking cows during the early stage of milk production is widely confident to be associated with PPH. In different parts of the world, many risk factors had been reported to be associated with PPH. Decreasing the level of phosphorous in blood serum, interrupted the phospholipid layers of red blood cells resulting in hemoglobinuria, a decrease in milk production, anorexia, and the death of animals in the cause if remained untreated. Urinalysis and hematology findings are the most commonly suitable diagnostic tools for this disease. Lots of studies have been suggesting that injection of sodium acid phosphate along with important minerals and supportive therapy with anti-oxidants can be used as the therapeutic protocol for the management of PPH.
产后血红蛋白尿(PPH)是一种散发的非传染性综合征,通常见于世界各地的牛。PPH最常见于妊娠早期和哺乳期早期的高产牛和水牛。PPH对阿富汗、巴基斯坦和印度的奶牛和水牛构成严重威胁,每年影响相当数量的动物。它的特点是血红蛋白尿、静脉溶血、严重贫血和因贫血和缺氧而死亡。然而,上述疾病的确切机制尚不完全清楚。为了找到导致上述疾病中出现血红蛋白尿的血管内溶血的确切原因,已经进行了大量和/或仍在进行的综合研究。但是,高产奶牛在产奶期早期的低磷血症被广泛认为与PPH有关。在世界不同地区,许多危险因素已被报道与PPH有关。降低血清中磷的水平,阻断红细胞的磷脂层,导致血红蛋白尿,产奶量减少,厌食症,如果不治疗,动物会死亡。尿液分析和血液学检查结果是本病最常用的诊断工具。大量研究表明,磷酸钠注射液联合重要矿物质和抗氧化剂的支持治疗可作为治疗PPH的治疗方案。
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兽医学(英文)
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