The detection of small molecule drugs is crucial in clinical treatment and environmental protection by facilitating the optimization of therapeutic regimens, preventing adverse drug reactions and monitoring environmental pollution. Electrochemiluminescence (ECL) is widely employed in the detection of small molecule drugs due to its high sensitivity and low background signal. This review highlights advancements from the last five years or so in ECL detection methods based on ECL reactions between luminophores and drugs as well as those based on affinity reactions between recognition molecules and drugs. Studies on affinity-based sensors including immunosensors, aptamer sensors, molecularly imprinted sensors, and composite material sensors are summarized. The review reveals that innovations in ECL luminophores, electrode materials and recognition materials are key areas of focus in this field. Nanomaterials play fundamentally important roles in enhancing the performance of ECL detection by acting as carriers of conventional luminophores, highly efficient luminescent materials, catalytically active electrode materials, and selective and stable recognition elements. With further advances in multiple drug detection, instrument miniaturization, on-site and point of care detection, and therapeutic monitoring, ECL is expected to play more significant roles in the detection of small molecule drugs.
{"title":"Recent advances in the electrochemiluminescence detection of small molecule drugs","authors":"Jiali Li, Xinni Yao, Jiateng Ma, Chuang Liu, Wenjun Hong, Haigang Wu, Minjie Li, Liang-Hong Guo","doi":"10.1039/d4an01562b","DOIUrl":"https://doi.org/10.1039/d4an01562b","url":null,"abstract":"The detection of small molecule drugs is crucial in clinical treatment and environmental protection by facilitating the optimization of therapeutic regimens, preventing adverse drug reactions and monitoring environmental pollution. Electrochemiluminescence (ECL) is widely employed in the detection of small molecule drugs due to its high sensitivity and low background signal. This review highlights advancements from the last five years or so in ECL detection methods based on ECL reactions between luminophores and drugs as well as those based on affinity reactions between recognition molecules and drugs. Studies on affinity-based sensors including immunosensors, aptamer sensors, molecularly imprinted sensors, and composite material sensors are summarized. The review reveals that innovations in ECL luminophores, electrode materials and recognition materials are key areas of focus in this field. Nanomaterials play fundamentally important roles in enhancing the performance of ECL detection by acting as carriers of conventional luminophores, highly efficient luminescent materials, catalytically active electrode materials, and selective and stable recognition elements. With further advances in multiple drug detection, instrument miniaturization, on-site and point of care detection, and therapeutic monitoring, ECL is expected to play more significant roles in the detection of small molecule drugs.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"48 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143477478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Guohao Xi, Jinmeng Su, Jie Ma, Lingzhi Wu, Jing Tu
Solid-state nanopores represent a powerful platform for the detection and characterization of a broader scope of biomolecules and particles, including proteins, viruses, and nanoparticles, for clinical and biochemical applications. Typically, nanopores work by measuring transient pulses of the ionic current as translocation events of molecules passing through the pore. In view of the strong noise and stochastic fluctuation of ionic current recording in nanopore experiments, the signal processing based on the statistical analysis of massive translocation events remains a crucial issue for nanopore sensing. Based on parallel computational processing and efficient memory management, we have developed a novel signal processing procedure for translocation events to improve the signal identification performance of solid-state nanopores in the presence of baseline oscillation interference. Obviously, by means of an adaptive threshold in a sliding window, we can correct the baseline determination process in real time. As a result, the features of the translocation event signals can be identified more accurately, especially for the intermittent occurrence of high-density complex signals, and the program also shows good signal differentiation. As a ready-to-use software, the data program is more efficient and compatible with diverse nanopore signals for more complex nanopore applications.
{"title":"A robust signal processing program for nanopore signals by dynamic correction threshold with compatible baseline fluctuations","authors":"Guohao Xi, Jinmeng Su, Jie Ma, Lingzhi Wu, Jing Tu","doi":"10.1039/d4an01384k","DOIUrl":"https://doi.org/10.1039/d4an01384k","url":null,"abstract":"Solid-state nanopores represent a powerful platform for the detection and characterization of a broader scope of biomolecules and particles, including proteins, viruses, and nanoparticles, for clinical and biochemical applications. Typically, nanopores work by measuring transient pulses of the ionic current as translocation events of molecules passing through the pore. In view of the strong noise and stochastic fluctuation of ionic current recording in nanopore experiments, the signal processing based on the statistical analysis of massive translocation events remains a crucial issue for nanopore sensing. Based on parallel computational processing and efficient memory management, we have developed a novel signal processing procedure for translocation events to improve the signal identification performance of solid-state nanopores in the presence of baseline oscillation interference. Obviously, by means of an adaptive threshold in a sliding window, we can correct the baseline determination process in real time. As a result, the features of the translocation event signals can be identified more accurately, especially for the intermittent occurrence of high-density complex signals, and the program also shows good signal differentiation. As a ready-to-use software, the data program is more efficient and compatible with diverse nanopore signals for more complex nanopore applications.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"25 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143477479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chenlong He, Huawei Liu, Ming Yin, Jing Chen, Wensi Huang, Han Zhou, Shengming Wu, Yilong Wang
Uric acid (UA) is a key end product of purine metabolism in the human body, and its abnormal level is associated with many diseases, so accurate monitoring is essential. Existing detection methods have many limitations, such as chromatography, which is cumbersome, time-consuming, and not cost-effective, and serum uric acid analysis, which requires specialized equipment and venous blood collection. In the field of uric acid sensors, electrochemical detection is commonly used but prone to interference, and nanomaterials are improved but complicated to modify. To better block the interference via an easily-made nanocomposites involved system, in this study, MnO2 with peroxidase-mimicking activity was used as a protective shell to encapsulate natural uric acid oxidase (UOx), realizing good combination of nanozymes and biocatalysts. The UOx can selectively catalyze UA and generate H2O2, and the MnO2 nanozymes can make up for the insufficiency of UOx, and the two main components synergistically make the activity of UOx@HMnO2 ultra-high, which can offer simple and versatile methods to prepare highly efficient hybrid biocatalysts for the fields of biosensors and biocatalysis. This provides a simple and general method for the preparation of efficient hybridized biocatalysts in the fields of biosensors and biocatalysis. The detection limit of the fabricated uric acid sensor is as low as 0.74 μM, and the concentration of the actual sample is consistent with that of mass spectrometry, which provides a means of non-invasive detection of uric acid with high sensitivity, high specificity and good accuracy.
{"title":"UOx@HMnO2 Biozyme-Nanozyme Driven Electrochemical Platform for Specific Uric Acid Bioassays","authors":"Chenlong He, Huawei Liu, Ming Yin, Jing Chen, Wensi Huang, Han Zhou, Shengming Wu, Yilong Wang","doi":"10.1039/d4an01512f","DOIUrl":"https://doi.org/10.1039/d4an01512f","url":null,"abstract":"Uric acid (UA) is a key end product of purine metabolism in the human body, and its abnormal level is associated with many diseases, so accurate monitoring is essential. Existing detection methods have many limitations, such as chromatography, which is cumbersome, time-consuming, and not cost-effective, and serum uric acid analysis, which requires specialized equipment and venous blood collection. In the field of uric acid sensors, electrochemical detection is commonly used but prone to interference, and nanomaterials are improved but complicated to modify. To better block the interference via an easily-made nanocomposites involved system, in this study, MnO2 with peroxidase-mimicking activity was used as a protective shell to encapsulate natural uric acid oxidase (UOx), realizing good combination of nanozymes and biocatalysts. The UOx can selectively catalyze UA and generate H2O2, and the MnO2 nanozymes can make up for the insufficiency of UOx, and the two main components synergistically make the activity of UOx@HMnO2 ultra-high, which can offer simple and versatile methods to prepare highly efficient hybrid biocatalysts for the fields of biosensors and biocatalysis. This provides a simple and general method for the preparation of efficient hybridized biocatalysts in the fields of biosensors and biocatalysis. The detection limit of the fabricated uric acid sensor is as low as 0.74 μM, and the concentration of the actual sample is consistent with that of mass spectrometry, which provides a means of non-invasive detection of uric acid with high sensitivity, high specificity and good accuracy.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"29 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143462679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juan Wang, Fan Zhang, Ziyang Liu, Yue Zhou, Liguo Pei, Bei Yan
This study presents a highly sensitive fluorescence biosensor integrating TDT enzyme and CRISPR-Cas12a for precise detection of sperm DNA damage, achieving a detection limit of 0.99 pM and a linear range of 0.001–0.2 nM. Its exceptional sensitivity, simplicity, and versatility make it a transformative tool for reproductive medicine and clinical diagnostics.
{"title":"A Novel Biosensor for Highly Sensitive DNA Damage Detection Using TdT and CRISPR-Cas12a","authors":"Juan Wang, Fan Zhang, Ziyang Liu, Yue Zhou, Liguo Pei, Bei Yan","doi":"10.1039/d5an00031a","DOIUrl":"https://doi.org/10.1039/d5an00031a","url":null,"abstract":"This study presents a highly sensitive fluorescence biosensor integrating TDT enzyme and CRISPR-Cas12a for precise detection of sperm DNA damage, achieving a detection limit of 0.99 pM and a linear range of 0.001–0.2 nM. Its exceptional sensitivity, simplicity, and versatility make it a transformative tool for reproductive medicine and clinical diagnostics.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"2 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Linlin Zhuang, Jiansen Gong, Di Zhang, Ping Zhang, Ying Zhao, Li Sun, Jianbo Yang, Yu Zhang, Qiuping Shen
The detection of E. coli is of irreplaceable importance for the maintenance of public health and food safety. In the field of molecular detection, metal and metal oxide nanoparticles have demonstrated significant advantages due to their unique physicochemical properties, and their application in E. coli detection has become a cutting-edge focus of scientific research. This review systematically introduces the innovative applications of these nanoparticles in E. coli detection, including the use of magnetic nanoparticles for efficient enrichment of bacteria and precise purification of nucleic acids, as well as a variety of nanoparticle-assisted immunoassays such as enzyme-linked immunosorbent assays, lateral flow immunoassays, colorimetric methods, and fluorescence strategies. In addition, this paper addresses the application of nanoparticles used in nucleic acid tests, including amplification-free and amplification-based assays. Further, the application of nanoparticles used in electrochemical and optical biosensors in E. coli detection is described, as well as other innovative assays. The advantages and challenges of aforementioned technologies are subjected to rigorous analysis, and a prospective outlook on the future direction of development is presented. In conclusion, this review not only illustrates the practical utility and extensive potential of metal and metal oxide nanoparticles in E. coli detection, but also serves as a scientific and comprehensive reference for molecular diagnostics in food safety and public health.
{"title":"Recent advances in the metallic and metal oxide nanoparticle-assisted molecular methods for the detection of Escherichia coli","authors":"Linlin Zhuang, Jiansen Gong, Di Zhang, Ping Zhang, Ying Zhao, Li Sun, Jianbo Yang, Yu Zhang, Qiuping Shen","doi":"10.1039/d4an01495b","DOIUrl":"https://doi.org/10.1039/d4an01495b","url":null,"abstract":"The detection of E. coli is of irreplaceable importance for the maintenance of public health and food safety. In the field of molecular detection, metal and metal oxide nanoparticles have demonstrated significant advantages due to their unique physicochemical properties, and their application in E. coli detection has become a cutting-edge focus of scientific research. This review systematically introduces the innovative applications of these nanoparticles in E. coli detection, including the use of magnetic nanoparticles for efficient enrichment of bacteria and precise purification of nucleic acids, as well as a variety of nanoparticle-assisted immunoassays such as enzyme-linked immunosorbent assays, lateral flow immunoassays, colorimetric methods, and fluorescence strategies. In addition, this paper addresses the application of nanoparticles used in nucleic acid tests, including amplification-free and amplification-based assays. Further, the application of nanoparticles used in electrochemical and optical biosensors in E. coli detection is described, as well as other innovative assays. The advantages and challenges of aforementioned technologies are subjected to rigorous analysis, and a prospective outlook on the future direction of development is presented. In conclusion, this review not only illustrates the practical utility and extensive potential of metal and metal oxide nanoparticles in E. coli detection, but also serves as a scientific and comprehensive reference for molecular diagnostics in food safety and public health.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"64 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143443788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sihua Wu, Jinhui Zou, Baohua Zhang, Jiantian Lu, Guan Rong Lin, Yuwei Zhang, Li Niu
Nickel oxide (NiO) nanozyme, as an excellent oxidase mimic, have been widely used in fluorescence biological detection, water pollutant analysis, food safety and cell imaging. However, to fully achieve these applications, it is still a great challenge to regulate its crystalline micro/nanostructure and composites to achieve high enzyme activity and high specific surface area. Here, we applied a very simple thermal annealing treatment to restructure the calcined precursor of NiO. Importantly, it was found that oxygen vacancy (OV) concentration of the targeted NiO nanozyme is significantly increased while the annealing atmosphere is argon rather than air. Moreover, the as-prepared novel NiO sample (NiO-OV) nanosheets achieved ca. 2-fold enhancement in specific surface area. It is believed that the higher OV concentration and the larger specific surface area will increase the enzyme activity by accelerating electron transfer rate and increasing catalytic interfaces. The significant improvement of enzyme activity of NiO-OV was verified by the fluorescence "turn-on" experiment of Amplex red (AR). Finally, using NiO-OV/AR system, we constructed a highly sensitive enzyme sensor on L-Cys with a detection limit of 37.8 nM. It also displayed an excellent specificity for ten typical amino acid interferences.
{"title":"Oxygen Vacancy-Enriched NiO Nanozymes Achieved by Facile Annealing in Argon for Detection of L-Cys","authors":"Sihua Wu, Jinhui Zou, Baohua Zhang, Jiantian Lu, Guan Rong Lin, Yuwei Zhang, Li Niu","doi":"10.1039/d5an00054h","DOIUrl":"https://doi.org/10.1039/d5an00054h","url":null,"abstract":"Nickel oxide (NiO) nanozyme, as an excellent oxidase mimic, have been widely used in fluorescence biological detection, water pollutant analysis, food safety and cell imaging. However, to fully achieve these applications, it is still a great challenge to regulate its crystalline micro/nanostructure and composites to achieve high enzyme activity and high specific surface area. Here, we applied a very simple thermal annealing treatment to restructure the calcined precursor of NiO. Importantly, it was found that oxygen vacancy (O<small><sub>V</sub></small>) concentration of the targeted NiO nanozyme is significantly increased while the annealing atmosphere is argon rather than air. Moreover, the as-prepared novel NiO sample (NiO-O<small><sub>V</sub></small>) nanosheets achieved ca. 2-fold enhancement in specific surface area. It is believed that the higher O<small><sub>V</sub></small> concentration and the larger specific surface area will increase the enzyme activity by accelerating electron transfer rate and increasing catalytic interfaces. The significant improvement of enzyme activity of NiO-O<small><sub>V </sub></small>was verified by the fluorescence \"turn-on\" experiment of Amplex red (AR). Finally, using NiO-O<small><sub>V</sub></small>/AR system, we constructed a highly sensitive enzyme sensor on L-Cys with a detection limit of 37.8 nM. It also displayed an excellent specificity for ten typical amino acid interferences.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"15 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143427113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mass spectrometry (MS)-based omics methods have transformed biomedical research with accurate and high-throughput analysis of diverse molecules in biological systems. Recent technological advances also enabled multi-omics to be achieved from the same sample or on a single analytical platform. Sample normalization is a critical step in MS-omics studies but is usually conducted independently for each omics experiment. To bridge this technical gap, we evaluated different sample normalization methods suitable for analyzing proteins, lipids, and metabolites from the same sample for multi-omics analysis. We found that normalizing samples based on tissue weight or protein concentration before or after extraction generated distinct quantitative results. Normalizing samples first by tissue weight before extraction and then by protein concentration after extraction resulted in the lowest sample variation to reveal true biological differences. We then applied this two-step normalization method to investigate multi-omics profiles of mouse brains lacking the GRN gene. Loss-of-function mutations in the GRN gene lead to the deficiency of the progranulin protein and eventually cause neurodegeneration. Comparing the proteomics, lipidomics, and metabolomics profiles of GRN KO and WT mouse brains revealed molecular changes and pathways related to lysosomal dysfunction and neuroinflammation. In summary, we demonstrated the importance of selecting an appropriate normalization method during multi-omics sample preparation. Our normalization method is applicable to all tissue-based multi-omics studies, ensuring reliable and accurate biomolecule quantification for biological comparisons.
{"title":"Evaluating sample normalization methods for MS-based multi-omics and the application to a neurodegenerative mouse model","authors":"Gwang Bin Lee, Cha Yang, Fenghua Hu, Ling Hao","doi":"10.1039/d4an01573h","DOIUrl":"https://doi.org/10.1039/d4an01573h","url":null,"abstract":"Mass spectrometry (MS)-based omics methods have transformed biomedical research with accurate and high-throughput analysis of diverse molecules in biological systems. Recent technological advances also enabled multi-omics to be achieved from the same sample or on a single analytical platform. Sample normalization is a critical step in MS-omics studies but is usually conducted independently for each omics experiment. To bridge this technical gap, we evaluated different sample normalization methods suitable for analyzing proteins, lipids, and metabolites from the same sample for multi-omics analysis. We found that normalizing samples based on tissue weight or protein concentration before or after extraction generated distinct quantitative results. Normalizing samples first by tissue weight before extraction and then by protein concentration after extraction resulted in the lowest sample variation to reveal true biological differences. We then applied this two-step normalization method to investigate multi-omics profiles of mouse brains lacking the GRN gene. Loss-of-function mutations in the GRN gene lead to the deficiency of the progranulin protein and eventually cause neurodegeneration. Comparing the proteomics, lipidomics, and metabolomics profiles of GRN KO and WT mouse brains revealed molecular changes and pathways related to lysosomal dysfunction and neuroinflammation. In summary, we demonstrated the importance of selecting an appropriate normalization method during multi-omics sample preparation. Our normalization method is applicable to all tissue-based multi-omics studies, ensuring reliable and accurate biomolecule quantification for biological comparisons.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"21 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143417999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiayan Liu, Taixue An, Jingjie Peng, Qinjiang Zhu, Heyang Zhao, Zhiyu Liang, Kai Mo, Tiancai Liu, Kun Wu
Most of the CRISPR-based RNA detection methods are combined with amplification to improve sensitivity, which lead to some drawbacks such as aerosol pollution, complicated operation, and amplification bias. To address the above issues, we developed a digital detection method for influenza A viral RNA based on droplet microfluidics and CRISPR/Cas13a without polymerase chain reaction. We used a microsphere coupled to a capture probe to extract and concentrate the target RNA from the samples, and then restricted the target-induced CRISPR/Cas13a cleavage event to microfluidic droplets, thus enhancing the local signal intensity and enabling single-molecule detection. With a detection limit of 10 copies per μL, influenza A viral RNA can be detected in less than 1 h. Both clinical and synthetic series samples were used to validate the assay's performance. With the help of this direct RNA diagnostic method, a variety of RNA molecules can be easily and accurately detected at the single-molecule level. This research has broad prospects in clinical applications.
{"title":"An amplification-free digital droplet assay for influenza A viral RNA based on CRISPR/Cas13a.","authors":"Jiayan Liu, Taixue An, Jingjie Peng, Qinjiang Zhu, Heyang Zhao, Zhiyu Liang, Kai Mo, Tiancai Liu, Kun Wu","doi":"10.1039/d4an01328j","DOIUrl":"https://doi.org/10.1039/d4an01328j","url":null,"abstract":"<p><p>Most of the CRISPR-based RNA detection methods are combined with amplification to improve sensitivity, which lead to some drawbacks such as aerosol pollution, complicated operation, and amplification bias. To address the above issues, we developed a digital detection method for influenza A viral RNA based on droplet microfluidics and CRISPR/Cas13a without polymerase chain reaction. We used a microsphere coupled to a capture probe to extract and concentrate the target RNA from the samples, and then restricted the target-induced CRISPR/Cas13a cleavage event to microfluidic droplets, thus enhancing the local signal intensity and enabling single-molecule detection. With a detection limit of 10 copies per μL, influenza A viral RNA can be detected in less than 1 h. Both clinical and synthetic series samples were used to validate the assay's performance. With the help of this direct RNA diagnostic method, a variety of RNA molecules can be easily and accurately detected at the single-molecule level. This research has broad prospects in clinical applications.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Correction for ‘Infrared spectromicroscopy of biochemistry in functional single cells’ by Luca Quaroni et al., Analyst, 2011, 136, 3219–3232, https://doi.org/10.1039/C1AN15060J.
{"title":"Correction: Infrared spectromicroscopy of biochemistry in functional single cells","authors":"Luca Quaroni and Theodora Zlateva","doi":"10.1039/D5AN90011E","DOIUrl":"10.1039/D5AN90011E","url":null,"abstract":"<p >Correction for ‘Infrared spectromicroscopy of biochemistry in functional single cells’ by Luca Quaroni <em>et al.</em>, <em>Analyst</em>, 2011, <strong>136</strong>, 3219–3232, https://doi.org/10.1039/C1AN15060J.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 5","pages":" 1022-1022"},"PeriodicalIF":3.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/an/d5an90011e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Infectious diseases have emerged as a significant global concern, posing a substantial burden in terms of the high morbidity and mortality, and presenting considerable challenges in clinical diagnosis and treatment. Therefore, it is highly-desired to develop new strategies for sensitive and accurate bacteria detection to address the global epidemic of antibiotic resistance. In this study, a new technique utilizing dual proximity ligation mediated chain extension and displacement strategy was developed for precise identification and highly sensitive detection of Methicillin-Resistant Staphylococcus Aureus (MRSA). The antibodies recognize both protein A and PBP2a on the surface of MRSA, leading to the initiation of proximity ligation and signal amplification process. The signal amplification procedure generated a substantial number of G-quadruplex sequences, which subsequently bind with thioflavin T (ThT) to significantly amplify its fluorescence, enabling the detection of MRSA with a low detection limit of 3.5 cfu/mL. In this method, dual proximity ligation assays were integrated to mediate the signal amplification process, thus endowing the method a greatly elevated specificity in both MRSA identification and signal amplification. Due to its non-label format, high selectivity, and sensitivity, this method can serve as a practical and versatile approach for detecting different bacteria in the early stages of infectious diseases.
{"title":"Dual Proximity Ligation Mediated Chain Extension and Displacement Assisted Signal Cycles for Sensitive and Accurate Methicillin-Resistant Staphylococcus Aureus (MRSA) Detection","authors":"Huali Xu, Xiangke Yang, Wen Wang, Xiaomin Yuan","doi":"10.1039/d5an00001g","DOIUrl":"https://doi.org/10.1039/d5an00001g","url":null,"abstract":"Infectious diseases have emerged as a significant global concern, posing a substantial burden in terms of the high morbidity and mortality, and presenting considerable challenges in clinical diagnosis and treatment. Therefore, it is highly-desired to develop new strategies for sensitive and accurate bacteria detection to address the global epidemic of antibiotic resistance. In this study, a new technique utilizing dual proximity ligation mediated chain extension and displacement strategy was developed for precise identification and highly sensitive detection of Methicillin-Resistant Staphylococcus Aureus (MRSA). The antibodies recognize both protein A and PBP2a on the surface of MRSA, leading to the initiation of proximity ligation and signal amplification process. The signal amplification procedure generated a substantial number of G-quadruplex sequences, which subsequently bind with thioflavin T (ThT) to significantly amplify its fluorescence, enabling the detection of MRSA with a low detection limit of 3.5 cfu/mL. In this method, dual proximity ligation assays were integrated to mediate the signal amplification process, thus endowing the method a greatly elevated specificity in both MRSA identification and signal amplification. Due to its non-label format, high selectivity, and sensitivity, this method can serve as a practical and versatile approach for detecting different bacteria in the early stages of infectious diseases.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"21 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：