Tao Huang, Jinxiang Xu, Chunsu Liang, Liyu Gong, Xiaomei Ling
Metal ions and their interactions with biomolecules play an important role in human health. However, optical detectors commonly used for HPCE cannot directly detect metal ions without UV absorption. To make up the shortcomings of existing HPCE detectors, a new universal HPCE detection system called interface-induced current detector (IICRD) has been constructed previously, with no need of derivatization procedures or complex instrumental modifications. Meanwhile, most of the reported studies on metal-biomolecule interactions only focused on the detection and analysis of biomolecules, commonly causing inaccurate or false-negative results, which was yet to be resolved. Here, the application of HPCE-IICRD realized the determination of metal-biomolecule interactions by directly measuring the electrophoretic parameters of metal ions for the first time, indicating that the interaction intensity can be measured more directly and accurately. Furthermore, an improved affinity capillary electrophoresis (ACE) method called relative mobility shift partial filling ACE-IICRD (rmsPF-ACE-IICRD) was originally developed to quantitatively analyze the binding strength. Binding behaviors between twelve free metal ions and three types of biomolecules (including two blood proteins, two enzyme proteins and two native DNAs) were investigated, and the values of equilibrium dissociation constant (KD) of metal-biomolecule complexes were calculated and evaluated by nonlinear chromatography (NLC) method. The experimental results were basically consistent with the literature values. In particular, heavy metal ions showed stronger interactions with proteins and enzymes, while metal ions tended to show stronger binding with native DNAs than proteins and enzymes, which were in agreement with literature results. The combined use of HPCE-IICRD and rmsPF-ACE showed great advantages such as no need of pretreatment, low operating cost, good repeatability, simple operation and no interference of coexisting substances, which is hopeful to become an efficient metal ion detection method, also to expand the application scope of IICRD in the future.
{"title":"Determination of metal-biomolecule interactions by relative mobility shift partial filling affinity capillary electrophoresis","authors":"Tao Huang, Jinxiang Xu, Chunsu Liang, Liyu Gong, Xiaomei Ling","doi":"10.1039/d4an01176g","DOIUrl":"https://doi.org/10.1039/d4an01176g","url":null,"abstract":"Metal ions and their interactions with biomolecules play an important role in human health. However, optical detectors commonly used for HPCE cannot directly detect metal ions without UV absorption. To make up the shortcomings of existing HPCE detectors, a new universal HPCE detection system called interface-induced current detector (IICRD) has been constructed previously, with no need of derivatization procedures or complex instrumental modifications. Meanwhile, most of the reported studies on metal-biomolecule interactions only focused on the detection and analysis of biomolecules, commonly causing inaccurate or false-negative results, which was yet to be resolved. Here, the application of HPCE-IICRD realized the determination of metal-biomolecule interactions by directly measuring the electrophoretic parameters of metal ions for the first time, indicating that the interaction intensity can be measured more directly and accurately. Furthermore, an improved affinity capillary electrophoresis (ACE) method called relative mobility shift partial filling ACE-IICRD (rmsPF-ACE-IICRD) was originally developed to quantitatively analyze the binding strength. Binding behaviors between twelve free metal ions and three types of biomolecules (including two blood proteins, two enzyme proteins and two native DNAs) were investigated, and the values of equilibrium dissociation constant (<em>K</em><small><sub>D</sub></small>) of metal-biomolecule complexes were calculated and evaluated by nonlinear chromatography (NLC) method. The experimental results were basically consistent with the literature values. In particular, heavy metal ions showed stronger interactions with proteins and enzymes, while metal ions tended to show stronger binding with native DNAs than proteins and enzymes, which were in agreement with literature results. The combined use of HPCE-IICRD and rmsPF-ACE showed great advantages such as no need of pretreatment, low operating cost, good repeatability, simple operation and no interference of coexisting substances, which is hopeful to become an efficient metal ion detection method, also to expand the application scope of IICRD in the future.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"12 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142645901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this work, a novel electrochemical sensor based on cobalt tungstate/multi-walled carbon nanotubes (CoWO4/MWCNTs) nanocomposite has been used to detect chlorpromazine hydrochloride (CPZ). The CoWO4/MWCNTs nanocomposite was obtained by solvothermal technology and ultrasonic method and analyzed via different characterization techniques of scanning electron microscope (SEM), X-ray diffractometer (XRD), energy dispersive spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS). The electrochemical behavior of CoWO4/MWCNTs/GCE was explored using cyclic voltammetry (CV). Electrochemical experiments have confirmed that CoWO4/MWCNTs/GCE exhibits excellent electrocatalytic activity towards CPZ with good selectivity, reproducibility and stability. The linear dynamic range of CPZ was observed to be 1-2000 µM with a detection limit of 0.33 µM. Moreover, the actual sample was analyzed using lake water with satisfactory results, which endows the sensor as potential candidates for the detection of CPZ.
{"title":"A sensitive electrochemical sensor based on CoWO4/multi-walled carbon nanotubes for the selective determination of chlorpromazine hydrochloride","authors":"Si Zeng, Peiyao Zhu, Deyu Liu, Yongmei Hu, Qitong Huang, Haiping Huang","doi":"10.1039/d4an01298d","DOIUrl":"https://doi.org/10.1039/d4an01298d","url":null,"abstract":"In this work, a novel electrochemical sensor based on cobalt tungstate/multi-walled carbon nanotubes (CoWO4/MWCNTs) nanocomposite has been used to detect chlorpromazine hydrochloride (CPZ). The CoWO4/MWCNTs nanocomposite was obtained by solvothermal technology and ultrasonic method and analyzed via different characterization techniques of scanning electron microscope (SEM), X-ray diffractometer (XRD), energy dispersive spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS). The electrochemical behavior of CoWO4/MWCNTs/GCE was explored using cyclic voltammetry (CV). Electrochemical experiments have confirmed that CoWO4/MWCNTs/GCE exhibits excellent electrocatalytic activity towards CPZ with good selectivity, reproducibility and stability. The linear dynamic range of CPZ was observed to be 1-2000 µM with a detection limit of 0.33 µM. Moreover, the actual sample was analyzed using lake water with satisfactory results, which endows the sensor as potential candidates for the detection of CPZ.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"162 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vincent Gaumerd, Yoan Capello, Quentin Bonnin, Pierre-Yves Renard, Anthony Romieu
Reaction-based fluorogenic sensing of lethal cyanide anion in aqueous matrices remains a big challenge. We have revisited the reported approach about intramolecular crossed-benzoin reaction leading to the release of a phenol-based fluorophore. Fluorescence assays and RP-HPLC-MS analyses have helped us to highlight its limitations related to poor aqueous stability of probes and impossibility to achieve molecular amplification despite the assumed catalytic activation mechanism. Traceless cleavable linker strategies were considered to obtain usable cyanide-responsive chemodosimeters and statistical analyses of fluorescence data have been deepened to accurately delineate their sensing performances, especially limit of detection (LOD).
{"title":"Fluorogenic detection of cyanide ions in pure aqueous media through intramolecular crossed-benzoin reaction: limitations unveiled and possible solutions","authors":"Vincent Gaumerd, Yoan Capello, Quentin Bonnin, Pierre-Yves Renard, Anthony Romieu","doi":"10.1039/d4an01368a","DOIUrl":"https://doi.org/10.1039/d4an01368a","url":null,"abstract":"Reaction-based fluorogenic sensing of lethal cyanide anion in aqueous matrices remains a big challenge. We have revisited the reported approach about intramolecular crossed-benzoin reaction leading to the release of a phenol-based fluorophore. Fluorescence assays and RP-HPLC-MS analyses have helped us to highlight its limitations related to poor aqueous stability of probes and impossibility to achieve molecular amplification despite the assumed catalytic activation mechanism. Traceless cleavable linker strategies were considered to obtain usable cyanide-responsive chemodosimeters and statistical analyses of fluorescence data have been deepened to accurately delineate their sensing performances, especially limit of detection (LOD).","PeriodicalId":63,"journal":{"name":"Analyst","volume":"9 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sensitive and accurate identification of interleukin-6 (IL-6) in biological fluids is very essential for judging migraine due to its role in different physiological and pathological processes. In this study, we designed a simple and feasible electrochemical immunosensing method for the voltammetric measurement of IL-6. The electrochemical immunosensor was fabricated through covalent conjugation of anti-IL-6 capture antibodies on the glassy carbon electrode with a typical carbodiimide coupling method. Anti-IL-6 secondary antibodies were labeled on the surface of Prussian blue-doped CaCO3 nanoparticle (PBCaNP) via the epoxy-amino reaction. The assay was carried out with a sandwich-type immunoreaction. In the presence of target IL-6, the analyst was sandwiched between capture antibody and detection antibody. Thereafter, the carried PBCaNP accompanying the secondary antibody could be determined by using square wave voltammetry (SWV). The voltammetric peak current was dependant on the concentration of target IL-6. Under optimum conditions, the electrochemical immunosensor exhibited good analytical properties, and allowed detection of IL-6 within a wide linear range from 0.1 to 1000 pg mL-1. The limit of detection was estimated to 0.078 pg mL-1 IL-6 at the 3sB criterion. An intermediate reproducibility of ≤10.59% was accomplished with batch-to-batch identification, and good anti-interferring capacity against other biomolecules was achieved. Importantly, clinical human serum samples obtained from 15 migraine patients were analyzed with the developed electrochemical immunosensors, giving well-matched results obtained from the referenced enzyme-linked immunosorbent assay (ELISA) method.
{"title":"Prussian blue-doped CaCO3 nanoparticle-labeled secondary antibodies for electrochemical immunoassay of interleukin-6 with migraine patients","authors":"Zhong Chen, Wenhui He, Renhe Lin, Dongzhi Wu, Xiaoling Jiang, Yunfan Cheng","doi":"10.1039/d4an01357c","DOIUrl":"https://doi.org/10.1039/d4an01357c","url":null,"abstract":"Sensitive and accurate identification of interleukin-6 (IL-6) in biological fluids is very essential for judging migraine due to its role in different physiological and pathological processes. In this study, we designed a simple and feasible electrochemical immunosensing method for the voltammetric measurement of IL-6. The electrochemical immunosensor was fabricated through covalent conjugation of anti-IL-6 capture antibodies on the glassy carbon electrode with a typical carbodiimide coupling method. Anti-IL-6 secondary antibodies were labeled on the surface of Prussian blue-doped CaCO3 nanoparticle (PBCaNP) via the epoxy-amino reaction. The assay was carried out with a sandwich-type immunoreaction. In the presence of target IL-6, the analyst was sandwiched between capture antibody and detection antibody. Thereafter, the carried PBCaNP accompanying the secondary antibody could be determined by using square wave voltammetry (SWV). The voltammetric peak current was dependant on the concentration of target IL-6. Under optimum conditions, the electrochemical immunosensor exhibited good analytical properties, and allowed detection of IL-6 within a wide linear range from 0.1 to 1000 pg mL-1. The limit of detection was estimated to 0.078 pg mL-1 IL-6 at the 3sB criterion. An intermediate reproducibility of ≤10.59% was accomplished with batch-to-batch identification, and good anti-interferring capacity against other biomolecules was achieved. Importantly, clinical human serum samples obtained from 15 migraine patients were analyzed with the developed electrochemical immunosensors, giving well-matched results obtained from the referenced enzyme-linked immunosorbent assay (ELISA) method.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"21 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
he oxidative-reduction electrochemiluminescence (ECL) potential of luminophore is one of the most significant indexes during the light generation processes to satisfy the growing demand for anti-interference analysis techniques, electrode compatibility and biological molecules damaging for the excessive excitation potential. The nanoparticle luminophores including quantum dots (QDs) and metal nanoclusters (NCs) processes tremendous potential for forming various ECL sensors due to their adjustable surface state. However, there are a few reviews focused on the nanoparticle luminophores based ECL system for low-triggering-potential (LTP) oxidative-reduction ECL to avoid the possible interference and oxidative damage of biological molecules. This review summarizes the recent advances of LTP oxidative-reduction ECL potential strategy of nanoparticle luminophores as ECL emitters, including matching efficient coreactant and nanoparticle luminophores, doping of nanoparticle luminophores, construction donor-acceptor system, choosing suitable working electrode, combining multiplex nanoparticle luminophores, and surface-engineering strategy. According to the different LTP ECL system, potential-lowing strategies and bio-related applications are discussed in detail. Moreover, the future trends and challenges of lowering ECL-triggering-potential strategies are discussed.
{"title":"Recent Advances and Future Prospects of Oxidative-Reduction Low-Triggering-Potential Electrochemiluminescence Strategy Based on Nanoparticle Luminophores","authors":"Li Fu, Tianyuan Song, Qi Li, Guizheng Zou, Fuwei Zhang, Zongchao Li, Haotian Guan, Yingshu Guo","doi":"10.1039/d4an01314j","DOIUrl":"https://doi.org/10.1039/d4an01314j","url":null,"abstract":"he oxidative-reduction electrochemiluminescence (ECL) potential of luminophore is one of the most significant indexes during the light generation processes to satisfy the growing demand for anti-interference analysis techniques, electrode compatibility and biological molecules damaging for the excessive excitation potential. The nanoparticle luminophores including quantum dots (QDs) and metal nanoclusters (NCs) processes tremendous potential for forming various ECL sensors due to their adjustable surface state. However, there are a few reviews focused on the nanoparticle luminophores based ECL system for low-triggering-potential (LTP) oxidative-reduction ECL to avoid the possible interference and oxidative damage of biological molecules. This review summarizes the recent advances of LTP oxidative-reduction ECL potential strategy of nanoparticle luminophores as ECL emitters, including matching efficient coreactant and nanoparticle luminophores, doping of nanoparticle luminophores, construction donor-acceptor system, choosing suitable working electrode, combining multiplex nanoparticle luminophores, and surface-engineering strategy. According to the different LTP ECL system, potential-lowing strategies and bio-related applications are discussed in detail. Moreover, the future trends and challenges of lowering ECL-triggering-potential strategies are discussed.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"19 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142609933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oxytocin (OXY), a peptide hormone and neurotransmitter essential to biological processes with nine distinct amino acid residues, has significantly received attention due to illegal use in food adulteration and stimulating milk ejection in cattle. Herein, for the first time, the electrochemical detection of Oxytocin (OXY) is reported using a novel nanocomposite consisting of a Zn-Cu metal-organic framework (Zn-Cu MOF) decorated on the reduced graphene oxide (rGO). An octahedral surface morphology with a crystalline structure of the size 45 nm, formation of a metal-oxygen bond, an enhanced pore diameter of 6.8 nm, a specific surface area of 70.8 m2/g, and pore volume of 0.08 cm3/g, revealed from the different characterization technique. The electro-catalytic behavior of Zn-Cu MOF/rGO nanocomposite has been increased substantially attributed to the synergistic effect, evident from the cyclic voltammetry (CV) when compared to Zn MOF, Cu MOF, Zn MOF/rGO, Cu MOF/rGO, Zn-Cu MOF keeping other parameters same. Moreover, the electrochemical impedance spectroscopy (EIS) spectra reveal the excellent conductivity of nanocomposite. The experimental parameters, viz. electrolyte pH (5), supporting electrolyte (0.1 M ABS), and volume of coating (12 µL), were optimized. The differential pulse voltammetry (DPV) technique was adopted to determine the OXY with the lowest limit of detection (LOD) to be 1.1 nM (S/N=3) with a linear range of 40 - 400 nM. The analytical application of the modified electrode was examined by spiking the OXY in pasteurized toned milk, skimmed powder milk, animal milk, and RO water, with a good recovery range of 95-106%.
{"title":"Tuning the electro-catalytic activity of the Zn-Cu MOF/rGO nanocomposite as a novel enzyme-free electrochemical sensor for the detection of Oxytocin hormone","authors":"Md Zainul Abedeen, Manish Sharma, Himmat Singh Kushwaha, Ragini Gupta","doi":"10.1039/d4an01157k","DOIUrl":"https://doi.org/10.1039/d4an01157k","url":null,"abstract":"Oxytocin (OXY), a peptide hormone and neurotransmitter essential to biological processes with nine distinct amino acid residues, has significantly received attention due to illegal use in food adulteration and stimulating milk ejection in cattle. Herein, for the first time, the electrochemical detection of Oxytocin (OXY) is reported using a novel nanocomposite consisting of a Zn-Cu metal-organic framework (Zn-Cu MOF) decorated on the reduced graphene oxide (rGO). An octahedral surface morphology with a crystalline structure of the size 45 nm, formation of a metal-oxygen bond, an enhanced pore diameter of 6.8 nm, a specific surface area of 70.8 m2/g, and pore volume of 0.08 cm3/g, revealed from the different characterization technique. The electro-catalytic behavior of Zn-Cu MOF/rGO nanocomposite has been increased substantially attributed to the synergistic effect, evident from the cyclic voltammetry (CV) when compared to Zn MOF, Cu MOF, Zn MOF/rGO, Cu MOF/rGO, Zn-Cu MOF keeping other parameters same. Moreover, the electrochemical impedance spectroscopy (EIS) spectra reveal the excellent conductivity of nanocomposite. The experimental parameters, viz. electrolyte pH (5), supporting electrolyte (0.1 M ABS), and volume of coating (12 µL), were optimized. The differential pulse voltammetry (DPV) technique was adopted to determine the OXY with the lowest limit of detection (LOD) to be 1.1 nM (S/N=3) with a linear range of 40 - 400 nM. The analytical application of the modified electrode was examined by spiking the OXY in pasteurized toned milk, skimmed powder milk, animal milk, and RO water, with a good recovery range of 95-106%.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"11 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cátia Santa, Soohyun Park, Artur Gejt, Heather A Clark, Bastian Hengerer, Khulan Sergelen
Real-time monitoring of therapeutic drugs is crucial for treatment management and pharmacokinetic studies. We present the optimization and affinity tuning of split-aptamer sandwich assay for real-time monitoring of the narrow therapeutic window drug vancomycin, using surface plasmon resonance (SPR). To achieve reversible, label-free sensing of small molecules by SPR, we adapted the vancomycin binding aptamer in a sandwich assay format through split-aptamer approach. By evaluating multiple split sites within the secondary structure of the original aptamer, we identified position 27 (P27) as optimal for preserving target affinity, ensuring reversibility, and maximizing sensitivity. The assay demonstrated robust performance under physiologically relevant ranges of pH and divalent cations and the specific ternary complex formation of the aptamer split segments with the analyte was confirmed by circular dichroism spectroscopy. Subsequently, we engineered a series of split-aptamer pairs with increasing complementarity in the stem regions, improving both the affinity and limit of detection up to 10-fold, as compared to the primary P27 pair. The kinetics of the engineered split-aptamer pairs were evaluated, revealing fast association and dissociation rates, confirming improved affinity and detection limits across variants. Most importantly, the reversibility of the assay, essential for real-time monitoring, was maintained in all pairs. Finally, the assay was further validated in complex biological matrices, including cerebrospinal fluid from dog and diluted plasma from rat, demonstrating functionality in biological environments, and stability exceeding 9 hours. Our study paves the way for applications of split-aptamers in real-time monitoring of small molecules, with potential implications for in vivo therapeutic drug monitoring and pharmacokinetic studies.
{"title":"Real-time monitoring of vancomycin using split-aptamer surface plasmon resonance biosensor","authors":"Cátia Santa, Soohyun Park, Artur Gejt, Heather A Clark, Bastian Hengerer, Khulan Sergelen","doi":"10.1039/d4an01226g","DOIUrl":"https://doi.org/10.1039/d4an01226g","url":null,"abstract":"Real-time monitoring of therapeutic drugs is crucial for treatment management and pharmacokinetic studies. We present the optimization and affinity tuning of split-aptamer sandwich assay for real-time monitoring of the narrow therapeutic window drug vancomycin, using surface plasmon resonance (SPR). To achieve reversible, label-free sensing of small molecules by SPR, we adapted the vancomycin binding aptamer in a sandwich assay format through split-aptamer approach. By evaluating multiple split sites within the secondary structure of the original aptamer, we identified position 27 (P27) as optimal for preserving target affinity, ensuring reversibility, and maximizing sensitivity. The assay demonstrated robust performance under physiologically relevant ranges of pH and divalent cations and the specific ternary complex formation of the aptamer split segments with the analyte was confirmed by circular dichroism spectroscopy. Subsequently, we engineered a series of split-aptamer pairs with increasing complementarity in the stem regions, improving both the affinity and limit of detection up to 10-fold, as compared to the primary P27 pair. The kinetics of the engineered split-aptamer pairs were evaluated, revealing fast association and dissociation rates, confirming improved affinity and detection limits across variants. Most importantly, the reversibility of the assay, essential for real-time monitoring, was maintained in all pairs. Finally, the assay was further validated in complex biological matrices, including cerebrospinal fluid from dog and diluted plasma from rat, demonstrating functionality in biological environments, and stability exceeding 9 hours. Our study paves the way for applications of split-aptamers in real-time monitoring of small molecules, with potential implications for in vivo therapeutic drug monitoring and pharmacokinetic studies.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"5 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142609905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jie Huang, Ruicheng Xu, Qiaoting Yang, kang Tao, Dan Shan
This study introduces a straightforward one-step pyrolytic method for synthesizing carbon dots derived from folic acid (FACDs). This green and cost-effective approach simplifies the production of fluorescent carbon nanomaterials, which exhibit strong and stable fluorescence properties. These properties make FACDs particularly suitable for the sensitive and selective detection of cysteine (Cys) through a "turn-on" fluorescence mechanism. The FACDs demonstrate a low detection limit of 1.7 × 10⁻⁷ M and a broad linear range from 5.0 × 10⁻⁷ to 8.0 × 10⁻ 5M, effectively restoring quenched fluorescence in the presence of Cys. These results underscore the potential of FACDs as reliable, high-performance sensors for various biological and environmental applications, paving the way for the development of versatile and efficient fluorescence-based detection platforms.
{"title":"Green One-step Pyrolytic Synthesis of Folic Acid-Derived Carbon Dots for Sensitive Turn-on Fluorescence Detection of Cysteine","authors":"Jie Huang, Ruicheng Xu, Qiaoting Yang, kang Tao, Dan Shan","doi":"10.1039/d4an01144a","DOIUrl":"https://doi.org/10.1039/d4an01144a","url":null,"abstract":"This study introduces a straightforward one-step pyrolytic method for synthesizing carbon dots derived from folic acid (FACDs). This green and cost-effective approach simplifies the production of fluorescent carbon nanomaterials, which exhibit strong and stable fluorescence properties. These properties make FACDs particularly suitable for the sensitive and selective detection of cysteine (Cys) through a \"turn-on\" fluorescence mechanism. The FACDs demonstrate a low detection limit of 1.7 × 10⁻⁷ M and a broad linear range from 5.0 × 10⁻⁷ to 8.0 × 10⁻ 5M, effectively restoring quenched fluorescence in the presence of Cys. These results underscore the potential of FACDs as reliable, high-performance sensors for various biological and environmental applications, paving the way for the development of versatile and efficient fluorescence-based detection platforms.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"20 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eirini Malegiannaki, Pascal Bohleber, Daniele Zannoni, Ciprian Stremtan, Agnese Petteni, Barbara Stenni, Carlo Barbante, Bo M. Vinther, Vasileios Gkinis
A new micro-destructive technique for high-resolution water isotope analysis of ice samples using a Laser Ablation (LA) system coupled with a Cavity Ring Down Spectrometer (CRDS) is presented. This method marks the first time water isotope analysis is conducted directly on the ice, bypassing the traditional steps of melting and vaporizing the ice sample, thanks to the direct transition of ice into water vapour through the laser ablation process. A nanosecond ArF laser ablation system (193 nm) with an integrated two-volume ablation chamber was successfully coupled to a CRDS analyzer, utilizing nitrogen as the carrier gas. The application goal is the use of LA-CRDS for ice core studies, so a method for preparing ice standard samples using liquid water isotope standards, widely used for ice core analysis, is introduced. The measurements were conducted in a discrete mode, by performing laser ablation raster scans of 4 mm × 4 mm areas, establishing a sampling resolution of 4 mm along an ice core's depth. The water vapour concentration reaching the CRDS analyzer as well as the quality of the water isotopic measurements of δ18O and δD were influenced by laser parameters, such as laser spot size, repetition rate, laser fluence, ablation time as well as by the flow rates of the carrier gas. After optimizing the experimental conditions for water vapour formation, three ice standards samples were analyzed for calibration purposes on the VSMOW-SLAP scale and a section of an ice core sample was also tested. Critical parameters influencing the precision and accuracy of water isotopic measurements were investigated, and isotopic fractionation phenomena were identified, pointing to essential considerations for the technique's further development.
{"title":"Towards high-resolution water isotope analysis in ice cores using laser ablation – cavity ring-down spectroscopy","authors":"Eirini Malegiannaki, Pascal Bohleber, Daniele Zannoni, Ciprian Stremtan, Agnese Petteni, Barbara Stenni, Carlo Barbante, Bo M. Vinther, Vasileios Gkinis","doi":"10.1039/d4an01054j","DOIUrl":"https://doi.org/10.1039/d4an01054j","url":null,"abstract":"A new micro-destructive technique for high-resolution water isotope analysis of ice samples using a Laser Ablation (LA) system coupled with a Cavity Ring Down Spectrometer (CRDS) is presented. This method marks the first time water isotope analysis is conducted directly on the ice, bypassing the traditional steps of melting and vaporizing the ice sample, thanks to the direct transition of ice into water vapour through the laser ablation process. A nanosecond ArF laser ablation system (193 nm) with an integrated two-volume ablation chamber was successfully coupled to a CRDS analyzer, utilizing nitrogen as the carrier gas. The application goal is the use of LA-CRDS for ice core studies, so a method for preparing ice standard samples using liquid water isotope standards, widely used for ice core analysis, is introduced. The measurements were conducted in a discrete mode, by performing laser ablation raster scans of 4 mm × 4 mm areas, establishing a sampling resolution of 4 mm along an ice core's depth. The water vapour concentration reaching the CRDS analyzer as well as the quality of the water isotopic measurements of <em>δ</em><small><sup>18</sup></small>O and <em>δ</em>D were influenced by laser parameters, such as laser spot size, repetition rate, laser fluence, ablation time as well as by the flow rates of the carrier gas. After optimizing the experimental conditions for water vapour formation, three ice standards samples were analyzed for calibration purposes on the VSMOW-SLAP scale and a section of an ice core sample was also tested. Critical parameters influencing the precision and accuracy of water isotopic measurements were investigated, and isotopic fractionation phenomena were identified, pointing to essential considerations for the technique's further development.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"9 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142600047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Md Raduanul H. Chowdhury, Clementinah Oladun, Nuwandi M. Ariyasingha, Anna Samoilenko, Tarek Bawardi, Dudari B. Burueva, Oleg G. Salnikov, Larisa M. Kovtunova, Valerii I. Bukhtiyarov, Zhongjie Shi, Kehuan Luo, Sidhartha Tan, Juri G. Gelovani, Igor V. Koptyug, Boyd M. Goodson, Eduard Y. Chekmenev
Proton-hyperpolarized contrast agents are attractive because they can be imaged on virtually any clinical MRI scanner, which is typically equipped to scan only protons rather than heteronuclei (i.e., anything besides protons, e.g., 13C, 15N, 129Xe, 23Na, etc.). Even though the lifetime of the proton spin hyperpolarization is only a few seconds, it is sufficient for inhalation and scanning of proton-hyperpolarized gas media. We demonstrate the utility of producing hyperpolarized propane gas via heterogeneous parahydrogen-induced polarization for the purpose of ventilation imaging in an excised rabbit lung model. The magnetization of protons in hyperpolarized propane gas is similar to that of tissue water protons, making it possible to rapidly perform lung ventilation imaging with a 0.35 T clinical MRI scanner. Here, we demonstrate the feasibility of rapid (2 s) lung ventilation MRI in excised rabbit lungs using hyperpolarized propane gas with a 1 × 1 mm2 pixel size using a 50 mm slice thickness, and a 1.7 × 1.7 mm2 pixel size using a 9 mm slice thickness.
{"title":"Rapid lung ventilation MRI using parahydrogen-induced polarization of propane gas","authors":"Md Raduanul H. Chowdhury, Clementinah Oladun, Nuwandi M. Ariyasingha, Anna Samoilenko, Tarek Bawardi, Dudari B. Burueva, Oleg G. Salnikov, Larisa M. Kovtunova, Valerii I. Bukhtiyarov, Zhongjie Shi, Kehuan Luo, Sidhartha Tan, Juri G. Gelovani, Igor V. Koptyug, Boyd M. Goodson, Eduard Y. Chekmenev","doi":"10.1039/d4an01029a","DOIUrl":"https://doi.org/10.1039/d4an01029a","url":null,"abstract":"Proton-hyperpolarized contrast agents are attractive because they can be imaged on virtually any clinical MRI scanner, which is typically equipped to scan only protons rather than heteronuclei (<em>i.e.</em>, anything besides protons, <em>e.g.</em>, <small><sup>13</sup></small>C, <small><sup>15</sup></small>N, <small><sup>129</sup></small>Xe, <small><sup>23</sup></small>Na, <em>etc</em>.). Even though the lifetime of the proton spin hyperpolarization is only a few seconds, it is sufficient for inhalation and scanning of proton-hyperpolarized gas media. We demonstrate the utility of producing hyperpolarized propane gas <em>via</em> heterogeneous parahydrogen-induced polarization for the purpose of ventilation imaging in an excised rabbit lung model. The magnetization of protons in hyperpolarized propane gas is similar to that of tissue water protons, making it possible to rapidly perform lung ventilation imaging with a 0.35 T clinical MRI scanner. Here, we demonstrate the feasibility of rapid (2 s) lung ventilation MRI in excised rabbit lungs using hyperpolarized propane gas with a 1 × 1 mm<small><sup>2</sup></small> pixel size using a 50 mm slice thickness, and a 1.7 × 1.7 mm<small><sup>2</sup></small> pixel size using a 9 mm slice thickness.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142600048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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