Nan Lin, Xiao Han, Shuo Tian, Dianping Tang, Danfeng Zhang
Interleukin-6 (IL-6) protects neurons by inhibiting the expression of factors related to neuronal injury in nervous headache patients. The development of rapid and sensitive IL-6 detection methods are very advantageous for easing the pain of the patients. In this work, an immobilization-free immunodetection method is explored for the voltammetric screening of IL-6 in serum samples from nervous headache patients. Initially, methylene blue-encapsulated liposome (MBLS) labeled with anti-IL-6 detection antibodies are confined into anti-IL-6 capture antibody-coated microplate through a sandwich-type immunoreaction, and subsequently subject to lysis treatment. After that, the lytic solution is transferred into a detection cell including Nafion-modified working electrode. Methylene blue molecules with positive charge are captured onto the negatively charged Nafion membrane, thus generating a voltammetric signal. The voltammetric peak currents are relative to the IL-6 amount in the solution. At the optimized experimental situations, MBLS-based split-type electrochemical sensing protocols have acceptable voltammetric currents for IL-6 from 0.01 to 100 pg mL-1, and allow screening of IL-6 as low as a concentration of 9.1 fg mL-1. The batch-to-batch coefficients of variation were ≤11.95%. A good anti-interference capability was acquired against other biomolecules. Seven human serum specimens and two diluted serum samples including IL-6 obtained from nervous headache patients were determined by MBLS-based electrochemical immunoassay, and achieved well-matched results in comparison with those of IL-6 ELISA protocol.
{"title":"Methylene blue-encapsulated liposome for immobilization-free electrochemical immunoassay of interleukin-6 from nervous headache","authors":"Nan Lin, Xiao Han, Shuo Tian, Dianping Tang, Danfeng Zhang","doi":"10.1039/d5an00346f","DOIUrl":"https://doi.org/10.1039/d5an00346f","url":null,"abstract":"Interleukin-6 (IL-6) protects neurons by inhibiting the expression of factors related to neuronal injury in nervous headache patients. The development of rapid and sensitive IL-6 detection methods are very advantageous for easing the pain of the patients. In this work, an immobilization-free immunodetection method is explored for the voltammetric screening of IL-6 in serum samples from nervous headache patients. Initially, methylene blue-encapsulated liposome (MBLS) labeled with anti-IL-6 detection antibodies are confined into anti-IL-6 capture antibody-coated microplate through a sandwich-type immunoreaction, and subsequently subject to lysis treatment. After that, the lytic solution is transferred into a detection cell including Nafion-modified working electrode. Methylene blue molecules with positive charge are captured onto the negatively charged Nafion membrane, thus generating a voltammetric signal. The voltammetric peak currents are relative to the IL-6 amount in the solution. At the optimized experimental situations, MBLS-based split-type electrochemical sensing protocols have acceptable voltammetric currents for IL-6 from 0.01 to 100 pg mL-1, and allow screening of IL-6 as low as a concentration of 9.1 fg mL-1. The batch-to-batch coefficients of variation were ≤11.95%. A good anti-interference capability was acquired against other biomolecules. Seven human serum specimens and two diluted serum samples including IL-6 obtained from nervous headache patients were determined by MBLS-based electrochemical immunoassay, and achieved well-matched results in comparison with those of IL-6 ELISA protocol.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"25 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143841615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The soluble amyloid-β oligomer (AβO) has been recognized as an important neuropathological hallmark for severity assessment of Alzheimer’s disease (AD) due to its more neurotoxicity than the larger and mature fibrillar Aβ. However, considering the time-scale aggregation pathway, AβO are transient, metastable and heterogeneous, developing reliable and straightforward methods able to directly monitor dynamics of AβO formation and conversion remains challenging. For this purpose, we fabricated a nanochannel array-based biosensor by immobilizing AβO aptamers (AβO-Apt) on the inner surface of the nanaochannels. The AβO-Apt could specificly bind AβO popoulations with high affinity, which would occur to growing rectified ion-current (at -1 V) within the nanochannels owing to lower nanospace occupancy of aptamers. Based on this principle, the nanochannel-aptamer-based (NC-Apt-based) biosensor we constructed could monitor the dynamics of AβO formation and conversion along with incubating timescale. Further investigation, we observed accelerating effects of external factors (such as increasing monomer concentrations and Al (Ⅲ) binding) on aggregation rate and state during AβO formation and conversion. This nanochannel-aptamer-based (NC-Apt-based) platform provides a chemically label-free and reliable assay to study dynamics of AβO formation and conversion without perturbing Aβ aggregation behavior. We expect it could be developed into a promising tool for estimating the toxicity degree of Aβ aggregates in clinical diagnosis.
{"title":"Nanochannel array-based platform for aggregation monitoring and high-sensitive identification of neurotoxic amyloid-β oligomers","authors":"Yilin Wang, Huang Liu, Yuzhen Zhao, Jiahui Chen, Xueni Li, Yong Shao, Dandan Wang","doi":"10.1039/d5an00233h","DOIUrl":"https://doi.org/10.1039/d5an00233h","url":null,"abstract":"The soluble amyloid-β oligomer (AβO) has been recognized as an important neuropathological hallmark for severity assessment of Alzheimer’s disease (AD) due to its more neurotoxicity than the larger and mature fibrillar Aβ. However, considering the time-scale aggregation pathway, AβO are transient, metastable and heterogeneous, developing reliable and straightforward methods able to directly monitor dynamics of AβO formation and conversion remains challenging. For this purpose, we fabricated a nanochannel array-based biosensor by immobilizing AβO aptamers (AβO-Apt) on the inner surface of the nanaochannels. The AβO-Apt could specificly bind AβO popoulations with high affinity, which would occur to growing rectified ion-current (at -1 V) within the nanochannels owing to lower nanospace occupancy of aptamers. Based on this principle, the nanochannel-aptamer-based (NC-Apt-based) biosensor we constructed could monitor the dynamics of AβO formation and conversion along with incubating timescale. Further investigation, we observed accelerating effects of external factors (such as increasing monomer concentrations and Al (Ⅲ) binding) on aggregation rate and state during AβO formation and conversion. This nanochannel-aptamer-based (NC-Apt-based) platform provides a chemically label-free and reliable assay to study dynamics of AβO formation and conversion without perturbing Aβ aggregation behavior. We expect it could be developed into a promising tool for estimating the toxicity degree of Aβ aggregates in clinical diagnosis.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"8 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143837538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wei Wei, Yiwei Zhang, Mengdi Yu, Di Shen, Xiyu Sun, Mingshi Fu, Chi Zhang, Zhenzhen Jia, Mingzhen Zhang
Dry eye disease (DED) is a prevalent condition characterized by a multifaceted etiology, with its incidence exhibiting an upward trajectory. Consequently, it is imperative to develop a sensitive, straightforward, and convenient method for the analysis of biomarkers associated with DED to facilitate its auxiliary diagnosis. Lysozyme (LYZ), produced by the lacrimal gland, is a class of antibacterial enzymes believed to play a crucial role in immunity and is associated with DED. In this study, a novel fluorescent sensing platform utilizing neutral red-heparin sodium (NR-HS) was developed with LYZ as the target. The platform operates on the principle of static quenching, where HS effectively quenches the fluorescence of NR. As a hydrolase, LYZ can catalyze the hydrolysis of the glucoside bond in HS, thereby modulating the transformation of NR-HS fluorescence signal. This provides a straightforward fluorescent method for monitoring LYZ levels. Under optimal conditions, the developed "on-off-on" NR-HS sensing platform demonstrated the capability to detect LYZ within a range of 0.5 to 10 µg/mL, with a detection limit of 0.42 µg/mL, and exhibited enhanced specificity for LYZ. In conclusion, a cost-effective, rapid, and efficient LYZ sensing platform was established, which facilitates the diagnosis of DED and shows potential as a diagnostic detection technique.
{"title":"A Novel Fluorescent Detection Strategy for Lysozyme in Tears Based on Glycoside Bond Hydrolysis","authors":"Wei Wei, Yiwei Zhang, Mengdi Yu, Di Shen, Xiyu Sun, Mingshi Fu, Chi Zhang, Zhenzhen Jia, Mingzhen Zhang","doi":"10.1039/d5an00331h","DOIUrl":"https://doi.org/10.1039/d5an00331h","url":null,"abstract":"Dry eye disease (DED) is a prevalent condition characterized by a multifaceted etiology, with its incidence exhibiting an upward trajectory. Consequently, it is imperative to develop a sensitive, straightforward, and convenient method for the analysis of biomarkers associated with DED to facilitate its auxiliary diagnosis. Lysozyme (LYZ), produced by the lacrimal gland, is a class of antibacterial enzymes believed to play a crucial role in immunity and is associated with DED. In this study, a novel fluorescent sensing platform utilizing neutral red-heparin sodium (NR-HS) was developed with LYZ as the target. The platform operates on the principle of static quenching, where HS effectively quenches the fluorescence of NR. As a hydrolase, LYZ can catalyze the hydrolysis of the glucoside bond in HS, thereby modulating the transformation of NR-HS fluorescence signal. This provides a straightforward fluorescent method for monitoring LYZ levels. Under optimal conditions, the developed \"on-off-on\" NR-HS sensing platform demonstrated the capability to detect LYZ within a range of 0.5 to 10 µg/mL, with a detection limit of 0.42 µg/mL, and exhibited enhanced specificity for LYZ. In conclusion, a cost-effective, rapid, and efficient LYZ sensing platform was established, which facilitates the diagnosis of DED and shows potential as a diagnostic detection technique.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"3 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143837539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veronika Tibljas, Simona Francese, Marjory Da Costa Abdreu, Robert Bradshaw
Inks, including ballpoint, gel, fountain pen, laser toner, inkjet, and security inks, have unique chemical formulations and require specific examination methods based on their properties and the surfaces they are deposited on. Despite the increasing use of digital signatures, ballpoint pens are still used for signing legal documents. Common forensic approaches involving visual examination are crucial in forensic analysis due to their non-destructive nature. However, often their effectiveness can rely on the expertise of the forensic analyst. Other common approaches, such as thin layer chromatography (TLC), require sample extraction which is destructive. This work shows a multimodal imaging workflow, including the application (in order of increasing destructiveness) of Desorption Electrospray Ionisation Mass Spectrometry Imaging (DESI MSI) followed by Matrix Assisted Laser Desorption Ionisation Mass Spectrometry Imaging (MALDI MSI) enabling comprehensive ink and substrate analysis in situ. The application of DESI MSI in negative ion mode and MALDI MSI in positive ion mode in principle allows for detection and mapping of diverse chemical species (e.g. dyes, polymers, etc), providing a comprehensive understanding of the chemical composition of the sample. Indeed, it is well documented that each ionisation technique can enable targeting of different molecular species. Statistical approaches used to interrogate the data, allows in-depth analysis of ink/substrates. This methodology has since been successfully used for a real casework example by our group. This offers a robust and reliable methodology, offering additional information for forgery determination in forensic cases, where results obtained from conventional methodologies may not have been successful.
{"title":"A multimodal mass spectrometry imaging workflow for ballpoint pen ink analysis and “forgery” detection","authors":"Veronika Tibljas, Simona Francese, Marjory Da Costa Abdreu, Robert Bradshaw","doi":"10.1039/d5an00217f","DOIUrl":"https://doi.org/10.1039/d5an00217f","url":null,"abstract":"Inks, including ballpoint, gel, fountain pen, laser toner, inkjet, and security inks, have unique chemical formulations and require specific examination methods based on their properties and the surfaces they are deposited on. Despite the increasing use of digital signatures, ballpoint pens are still used for signing legal documents. Common forensic approaches involving visual examination are crucial in forensic analysis due to their non-destructive nature. However, often their effectiveness can rely on the expertise of the forensic analyst. Other common approaches, such as thin layer chromatography (TLC), require sample extraction which is destructive. This work shows a multimodal imaging workflow, including the application (in order of increasing destructiveness) of Desorption Electrospray Ionisation Mass Spectrometry Imaging (DESI MSI) followed by Matrix Assisted Laser Desorption Ionisation Mass Spectrometry Imaging (MALDI MSI) enabling comprehensive ink and substrate analysis in situ. The application of DESI MSI in negative ion mode and MALDI MSI in positive ion mode in principle allows for detection and mapping of diverse chemical species (e.g. dyes, polymers, etc), providing a comprehensive understanding of the chemical composition of the sample. Indeed, it is well documented that each ionisation technique can enable targeting of different molecular species. Statistical approaches used to interrogate the data, allows in-depth analysis of ink/substrates. This methodology has since been successfully used for a real casework example by our group. This offers a robust and reliable methodology, offering additional information for forgery determination in forensic cases, where results obtained from conventional methodologies may not have been successful.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"2 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143837537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jia-Yuan Li, Sen Zhang, Wan-yue Zhuang, Feng Zhang, Wei-juan Zheng, Li Mao, Hong-Zhen Lian
Mesopore-structured cobalt and nickel metal-organic frameworks (Co/Ni-ZIF) were synthesized by self-assembly method using Co and Ni as the bimetal centers and 2-methylimidazole as the organic ligand at room temperature. The resulting rhombic dodecahedral nanocomposites possessing rich mesopores with the average diameter of 4 nm were collected centrifugally and then carbonized under a nitrogen atmosphere to generate bimetallic magnetic porous carbon nanocomposites (Co/Ni-MCNs). After thorough characterization, the as-prepared Co/Ni-MCNs decorated by graphite shell layer with pyridinic nitrogen were utilized in magnetic separation and enrichment of low-abundance peptides through hydrophobic and π-π stacking interactions. This is the first attempt to prepare the mesoporous carbon materials having plentiful holes with bimetal MOF as precursors in which partial nickel-containing components served for the sacrificial templates. Owing to the ordered structure, abundant mesopores, rich interaction sites, excellent magnetic property and good compatibility to biological tissues, this proposed magnetic affinity probe has been successfully used in the identification of endogenous peptides in human urine and serum in combination with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS).
{"title":"Magnetic Mesoporous Carbon Nanocomposites Derived from Bimetallic Metal-organic Framework for Enrichment of Low-abundance Peptides","authors":"Jia-Yuan Li, Sen Zhang, Wan-yue Zhuang, Feng Zhang, Wei-juan Zheng, Li Mao, Hong-Zhen Lian","doi":"10.1039/d5an00127g","DOIUrl":"https://doi.org/10.1039/d5an00127g","url":null,"abstract":"Mesopore-structured cobalt and nickel metal-organic frameworks (Co/Ni-ZIF) were synthesized by self-assembly method using Co and Ni as the bimetal centers and 2-methylimidazole as the organic ligand at room temperature. The resulting rhombic dodecahedral nanocomposites possessing rich mesopores with the average diameter of 4 nm were collected centrifugally and then carbonized under a nitrogen atmosphere to generate bimetallic magnetic porous carbon nanocomposites (Co/Ni-MCNs). After thorough characterization, the as-prepared Co/Ni-MCNs decorated by graphite shell layer with pyridinic nitrogen were utilized in magnetic separation and enrichment of low-abundance peptides through hydrophobic and π-π stacking interactions. This is the first attempt to prepare the mesoporous carbon materials having plentiful holes with bimetal MOF as precursors in which partial nickel-containing components served for the sacrificial templates. Owing to the ordered structure, abundant mesopores, rich interaction sites, excellent magnetic property and good compatibility to biological tissues, this proposed magnetic affinity probe has been successfully used in the identification of endogenous peptides in human urine and serum in combination with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS).","PeriodicalId":63,"journal":{"name":"Analyst","volume":"34 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yue Cao, Longfei Lai, Qingyuan Bao, Yiru Sheng, Peng Ye, Zhaogang Teng, Yang Zhou
In recent years, the overuse and improper disposal of antibiotics have led to their widespread presence in various environmental matrices and biological fluids. Exploring non-noble metal sulfides as electrocatalysts for reliable antibiotic detection holds substantial value but is limited by their inherent low electrochemical activity. Herein, CoS2 nanoparticles were first synthesized via a hydrothermal method, and used for the in situ assembly of ZnIn2S4 nanosheets. Then, plasma technology was employed for efficient and rapid generation of sulfur vacancies. Detailed studies illustrated the formation of heterojunctions, facilitating electron transfer from the thin, large-area ZnIn2S4 to CoS2, which acts as a co-catalyst due to its inherent conductivity and metallicity. The plasma-assisted generation of vacancies resulted in the formation of numerous exposed metal catalytic centers. Also, both the heterojunction and vacancy construction afforded a narrowed energy gap, an upregulated conduction band, and an enhanced carrier density, favoring electron injection during the electro-reduction process. Thus, an electrochemical platform was established using this material as an electrode substrate for furazolidone (Fz) detection, achieving a detection limit of 1.2 nM. The constructed platform was finally applied to evaluate Fz in actual samples of river water and urine, thanks to its good selectivity, high stability, and acceptable repeatability. In addition, in situ Fourier transform infrared technology was also used to monitor the electrocatalytic process. This work paves the way for preparing low-cost non-precious metal sulfides, which can be used as promising candidates for electrocatalysts in antibiotic testing.
{"title":"Strategic engineering of CoS2/ZnIn2S4 heterostructures incorporating Ar plasma-induced sulfur vacancies for potentiating electrocatalytic activity in furazolidone determination","authors":"Yue Cao, Longfei Lai, Qingyuan Bao, Yiru Sheng, Peng Ye, Zhaogang Teng, Yang Zhou","doi":"10.1039/d5an00288e","DOIUrl":"https://doi.org/10.1039/d5an00288e","url":null,"abstract":"In recent years, the overuse and improper disposal of antibiotics have led to their widespread presence in various environmental matrices and biological fluids. Exploring non-noble metal sulfides as electrocatalysts for reliable antibiotic detection holds substantial value but is limited by their inherent low electrochemical activity. Herein, CoS<small><sub>2</sub></small> nanoparticles were first synthesized <em>via</em> a hydrothermal method, and used for the <em>in situ</em> assembly of ZnIn<small><sub>2</sub></small>S<small><sub>4</sub></small> nanosheets. Then, plasma technology was employed for efficient and rapid generation of sulfur vacancies. Detailed studies illustrated the formation of heterojunctions, facilitating electron transfer from the thin, large-area ZnIn<small><sub>2</sub></small>S<small><sub>4</sub></small> to CoS<small><sub>2</sub></small>, which acts as a co-catalyst due to its inherent conductivity and metallicity. The plasma-assisted generation of vacancies resulted in the formation of numerous exposed metal catalytic centers. Also, both the heterojunction and vacancy construction afforded a narrowed energy gap, an upregulated conduction band, and an enhanced carrier density, favoring electron injection during the electro-reduction process. Thus, an electrochemical platform was established using this material as an electrode substrate for furazolidone (Fz) detection, achieving a detection limit of 1.2 nM. The constructed platform was finally applied to evaluate Fz in actual samples of river water and urine, thanks to its good selectivity, high stability, and acceptable repeatability. In addition, <em>in situ</em> Fourier transform infrared technology was also used to monitor the electrocatalytic process. This work paves the way for preparing low-cost non-precious metal sulfides, which can be used as promising candidates for electrocatalysts in antibiotic testing.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"25 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143827534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vanillylmandelic acid (VMA) and homovanillic acid (HVA) are biomarkers for the diagnosis and course-of-disease monitoring of malignant tumor neuroblastomas, which endanger infants and children. Herein, we demonstrated a proof-of-concept visual detection of VMA and HVA on an electrochromic basis, in which the viologen 1,1′-dibenzyl-4,4′-bipyridinium dichloride was used as a coloration chromophore. It was found that VMA and HVA can be used as effective electron mediators to improve the electrochromic performance of devices. It is interesting to note that VMA and HVA reduce the driving voltage of electrochromic devices (ECDs) down to −1.0 V, which is lower than that (−2.1 V) achieved without these additives, and the coloration of ECDs is undoubtedly dependent on the concentration of VMA and HVA from 0.8 to 10−6 mol L−1. Thus, this study presents an ECD platform as a breakthrough strategy for the facile, routine and portable visual detection of the neuroblastoma biomarkers VMA and HVA with obvious advantages over other detection techniques such as HPLC/MS used in clinical diagnosis.
{"title":"Electrochromic platform for the visual detection of the neuroblastoma biomarkers vanillylmandelic acid and homovanillic acid","authors":"Lu Li, Xiaodi Liu, Shijie Xu, Shiming Zhang, Zhuangzhuang Yang, Debao Xiao","doi":"10.1039/d5an00228a","DOIUrl":"https://doi.org/10.1039/d5an00228a","url":null,"abstract":"Vanillylmandelic acid (VMA) and homovanillic acid (HVA) are biomarkers for the diagnosis and course-of-disease monitoring of malignant tumor neuroblastomas, which endanger infants and children. Herein, we demonstrated a proof-of-concept visual detection of VMA and HVA on an electrochromic basis, in which the viologen 1,1′-dibenzyl-4,4′-bipyridinium dichloride was used as a coloration chromophore. It was found that VMA and HVA can be used as effective electron mediators to improve the electrochromic performance of devices. It is interesting to note that VMA and HVA reduce the driving voltage of electrochromic devices (ECDs) down to −1.0 V, which is lower than that (−2.1 V) achieved without these additives, and the coloration of ECDs is undoubtedly dependent on the concentration of VMA and HVA from 0.8 to 10<small><sup>−6</sup></small> mol L<small><sup>−1</sup></small>. Thus, this study presents an ECD platform as a breakthrough strategy for the facile, routine and portable visual detection of the neuroblastoma biomarkers VMA and HVA with obvious advantages over other detection techniques such as HPLC/MS used in clinical diagnosis.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"75 6 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143827533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yijiao Qu, Ming Chen, Mufeng Han, Xiaoyu Yu, Xi Yu, Jinghan Fan, Huihui Liu, Liping Wang, Zongxiu Nie
Infertility is a significant challenge faced by many families worldwide, with recurrent pregnancy loss (RPL) being a prevalent cause of infertility among women. This condition causes immense emotional and physical distress for affected individuals and their families. In this study, we present a rapid, efficient, and high-throughput analytical method using PS@Fe3O4-NH2 magnetic beads as a matrix for the detection of urinary metabolite fingerprints in RPL patients via laser desorption/ionization mass spectrometry (LDI-MS) combined with machine learning (ML). This approach offers rich metabolic information from urine samples, through subsequent analysis we identify 17 metabolites that significantly differ between RPL patients and healthy controls (HC). The application of mass spectrometry features in conjunction with ML enabled effective screening of RPL patients and the identification of dysregulated metabolic pathways. This method presents a promising, non-invasive, and rapid screening approach for early detection of RPL, facilitating timely intervention and contributing to women's health.
{"title":"High throughput recurrent pregnancy loss screening: urine metabolic fingerprints via LDI-MS and machine learning","authors":"Yijiao Qu, Ming Chen, Mufeng Han, Xiaoyu Yu, Xi Yu, Jinghan Fan, Huihui Liu, Liping Wang, Zongxiu Nie","doi":"10.1039/d5an00177c","DOIUrl":"https://doi.org/10.1039/d5an00177c","url":null,"abstract":"Infertility is a significant challenge faced by many families worldwide, with recurrent pregnancy loss (RPL) being a prevalent cause of infertility among women. This condition causes immense emotional and physical distress for affected individuals and their families. In this study, we present a rapid, efficient, and high-throughput analytical method using PS@Fe<small><sub>3</sub></small>O<small><sub>4</sub></small>-NH<small><sub>2</sub></small> magnetic beads as a matrix for the detection of urinary metabolite fingerprints in RPL patients <em>via</em> laser desorption/ionization mass spectrometry (LDI-MS) combined with machine learning (ML). This approach offers rich metabolic information from urine samples, through subsequent analysis we identify 17 metabolites that significantly differ between RPL patients and healthy controls (HC). The application of mass spectrometry features in conjunction with ML enabled effective screening of RPL patients and the identification of dysregulated metabolic pathways. This method presents a promising, non-invasive, and rapid screening approach for early detection of RPL, facilitating timely intervention and contributing to women's health.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"25 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143819043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
mengjie yu, Yuting Liu, Jianhui Xiong, longfei miao, Li Wang
Acetaminophen (APAP), also known as paracetamol, is a widely used analgesic and antipyretic, but its metabolites are toxic and can cause liver damage when used in excess. Rapid detection of APAP is essential, and conventional methods such as HPLC and GC are expensive and complex. To this purpose, we successfully prepared CoO/Co3O4/NC porous carbon composites with large specific surface area, homogeneous pore structure, and abundant adsorption active sites as electrochemical sensors for the rapid, simple, and inexpensive detection of acetaminophen. The CoO/Co3O4/NC porous carbon composites were prepared by doping Co2+ and calcining, and a large number of N and O metal chelate sites in COFTZT-DVA could coordinate with Co2+, which effectively suppressed the aggregation phenomenon of CoO/Co3O4 in the composites, and realized the uniform dispersion of Co2+. This composite material exhibits both excellent stability and catalytic performance. The experimental results showed that the sensor had an extremely low detection limit (0.79 μM) and a wide linear response range (2.5 μM-423 μM). This study provides a new strategy for the preparation of high-performance paracetamol sensors.
{"title":"Electrochemical detection of Paracetamol based on CoO/Co3O4/NC nanocomposites derived from COFs","authors":"mengjie yu, Yuting Liu, Jianhui Xiong, longfei miao, Li Wang","doi":"10.1039/d5an00193e","DOIUrl":"https://doi.org/10.1039/d5an00193e","url":null,"abstract":"Acetaminophen (APAP), also known as paracetamol, is a widely used analgesic and antipyretic, but its metabolites are toxic and can cause liver damage when used in excess. Rapid detection of APAP is essential, and conventional methods such as HPLC and GC are expensive and complex. To this purpose, we successfully prepared CoO/Co3O4/NC porous carbon composites with large specific surface area, homogeneous pore structure, and abundant adsorption active sites as electrochemical sensors for the rapid, simple, and inexpensive detection of acetaminophen. The CoO/Co3O4/NC porous carbon composites were prepared by doping Co2+ and calcining, and a large number of N and O metal chelate sites in COFTZT-DVA could coordinate with Co2+, which effectively suppressed the aggregation phenomenon of CoO/Co3O4 in the composites, and realized the uniform dispersion of Co2+. This composite material exhibits both excellent stability and catalytic performance. The experimental results showed that the sensor had an extremely low detection limit (0.79 μM) and a wide linear response range (2.5 μM-423 μM). This study provides a new strategy for the preparation of high-performance paracetamol sensors.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"4 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143819117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Odorous gas emission is one of the world's seven major public nuisances, easily causing disturbances and complaints, and posing a threat to air quality and public health. Emissions of odorous substances are characterized by their sudden and instantaneous nature, with some odorous compounds having extremely low olfactory thresholds. Therefore, it is essential to develop highly sensitive on-site rapid detection techniques. This paper presents a new technology that combines a cluster hollow fiber membrane (CHFM) with mobile proton transfer reaction mass spectrometry (PTR-MS), developing a cluster hollow fiber membrane-proton transfer reaction mass spectrometry (CHFM-PTR-MS) technique. Due to the large membrane area (1000 cm2) of the cluster hollow fiber membrane module, the CHFM-PTR-MS shows improved sensitivity for eight tested odorous substances by 7.6 to 12.2 times and an enhanced limit of detection by 6.7 to 12.4 times compared to traditional direct-injection PTR-MS. Through a 28-hour continuous monitoring experiment of odorous gases released from household waste, the capability of the new CHFM-PTR-MS technology for on-site rapid detection of trace odorous organic compounds was verified. The CHFM-PTR-MS is expected to provide a new technique and device for on-site rapid detection of odorous organic compounds with high sensitivity.
{"title":"Research on cluster hollow fiber membrane proton transfer reaction mass spectrometry (CHFM-PTR-MS) and its application in odorous gas detection.","authors":"Feiyang Sun, Xun Bao, Qiangling Zhang, Jie Jin, Qian Xia, Kexin Gu, Qu Liang, Wei Xu, Xue Zou, Chaoqun Huang, Chengyin Shen, Yannan Chu","doi":"10.1039/d5an00084j","DOIUrl":"https://doi.org/10.1039/d5an00084j","url":null,"abstract":"<p><p>Odorous gas emission is one of the world's seven major public nuisances, easily causing disturbances and complaints, and posing a threat to air quality and public health. Emissions of odorous substances are characterized by their sudden and instantaneous nature, with some odorous compounds having extremely low olfactory thresholds. Therefore, it is essential to develop highly sensitive on-site rapid detection techniques. This paper presents a new technology that combines a cluster hollow fiber membrane (CHFM) with mobile proton transfer reaction mass spectrometry (PTR-MS), developing a cluster hollow fiber membrane-proton transfer reaction mass spectrometry (CHFM-PTR-MS) technique. Due to the large membrane area (1000 cm<sup>2</sup>) of the cluster hollow fiber membrane module, the CHFM-PTR-MS shows improved sensitivity for eight tested odorous substances by 7.6 to 12.2 times and an enhanced limit of detection by 6.7 to 12.4 times compared to traditional direct-injection PTR-MS. Through a 28-hour continuous monitoring experiment of odorous gases released from household waste, the capability of the new CHFM-PTR-MS technology for on-site rapid detection of trace odorous organic compounds was verified. The CHFM-PTR-MS is expected to provide a new technique and device for on-site rapid detection of odorous organic compounds with high sensitivity.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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