Cymoril COMBESCOT, Olivier Piot, Valérie Untereiner, Anne Durlach, Francesco Laconi
Hirschsprung disease is a congenital anomaly characterised by an absence of innervation inf the colon. The current diagnosis, consisting in identifying the unfunctional part of the colon by histological examination is unsatisfactory. The objective of our study was to assess the potential of infrared spectroscopy as a label-free method to distinguish between functional and unfunctional parts of the colon. From FFPE tissues sections from Hirschsprung patients, tissue samples at both functional and unfunctional regions were analysed by mid-infrared imaging. Colour-coded spectral images, reconstructed by means of multivariate data processing, were compared to the gold standard (hematoxylin-eosin-safran staining) to associate a specific spectral signature to each specific histological structure. Statical analyses were also carried out to highlight infrared markers associated with Hirschsprung disease. The research of ganglion cells and cholinergic threads, the usuals makers of the disease, was unsuccessful. However, our approach was efficient in differentiating between functional and unfunctional parts of the colon by focussing on the muscularis. As such, vibrational spectroscopy can highlight biochemical differences that are not visible using standard histology. This proof-of-concept study suggests that vibrational spectroscopy is a candidate method to diagnose Hirschsprung disease, paving the way to intraoperative application by helping surgeons and histologists in the delineation of the pathological region.
{"title":"TISSUE ANALYSIS BY VIBRATIONAL SPECTROSCOPY IN HIRSCHSPRUNG’S DISEASE: FEASIBILITY AND POTENTIAL AS A NEW INTRAOPERATIVE TOOL","authors":"Cymoril COMBESCOT, Olivier Piot, Valérie Untereiner, Anne Durlach, Francesco Laconi","doi":"10.1039/d4an01489h","DOIUrl":"https://doi.org/10.1039/d4an01489h","url":null,"abstract":"Hirschsprung disease is a congenital anomaly characterised by an absence of innervation inf the colon. The current diagnosis, consisting in identifying the unfunctional part of the colon by histological examination is unsatisfactory. The objective of our study was to assess the potential of infrared spectroscopy as a label-free method to distinguish between functional and unfunctional parts of the colon. From FFPE tissues sections from Hirschsprung patients, tissue samples at both functional and unfunctional regions were analysed by mid-infrared imaging. Colour-coded spectral images, reconstructed by means of multivariate data processing, were compared to the gold standard (hematoxylin-eosin-safran staining) to associate a specific spectral signature to each specific histological structure. Statical analyses were also carried out to highlight infrared markers associated with Hirschsprung disease. The research of ganglion cells and cholinergic threads, the usuals makers of the disease, was unsuccessful. However, our approach was efficient in differentiating between functional and unfunctional parts of the colon by focussing on the muscularis. As such, vibrational spectroscopy can highlight biochemical differences that are not visible using standard histology. This proof-of-concept study suggests that vibrational spectroscopy is a candidate method to diagnose Hirschsprung disease, paving the way to intraoperative application by helping surgeons and histologists in the delineation of the pathological region.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"132 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143375775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The best antimicrobials and cancer therapies intercalate DNA-binding compounds to target DNA-processing proteins. We developed a DNA intercalator ligand that addresses the aggregation to speed up the development of novel intercalating medications. This DNA-intercalating ligand allows cellular imaging with little background interference due to its aggregation-induced emission (AIE) and colorimetric pH-responsiveness. These properties make it a versatile biosensor. The ligand exhibits robust intercalative binding with DNA at 52.6 nM concentrations and a high affinity of 55.46×106 M-1, ensuring sensitivity to low DNA concentrations. By changing its emission intensity and spectrum features with viscosity, the ligand may sense its microenvironment's physical parameters. It also changes color when pH changes, making pH monitoring easy and noticeable. We studied its DNA interaction using absorbance, fluorescence, and circular dichroism spectroscopy. To determine the mechanism, we performed dye-displacement tests, melting temperature investigations, and iodide quenching. RED fluorescence, high-affinity DNA intercalation, aggregation-induced emission (AIE), viscosity sensitivity, and pH-dependent colorimetric response make this ligand a promising candidate for DNA-targeted imaging and pH-sensitive biosensing in biological and environmental applications. Plant cell tissue imaging has also proved successful.
{"title":"Multifunctional Red-Emissive AIEgens as DNA-Intercalating Ligand: Dual Sensing of pH and Viscosity Accompanied by Tissue Imaging","authors":"Sulekha Kumari Pandit, Gopal Das","doi":"10.1039/d5an00025d","DOIUrl":"https://doi.org/10.1039/d5an00025d","url":null,"abstract":"The best antimicrobials and cancer therapies intercalate DNA-binding compounds to target DNA-processing proteins. We developed a DNA intercalator ligand that addresses the aggregation to speed up the development of novel intercalating medications. This DNA-intercalating ligand allows cellular imaging with little background interference due to its aggregation-induced emission (AIE) and colorimetric pH-responsiveness. These properties make it a versatile biosensor. The ligand exhibits robust intercalative binding with DNA at 52.6 nM concentrations and a high affinity of 55.46×106 M-1, ensuring sensitivity to low DNA concentrations. By changing its emission intensity and spectrum features with viscosity, the ligand may sense its microenvironment's physical parameters. It also changes color when pH changes, making pH monitoring easy and noticeable. We studied its DNA interaction using absorbance, fluorescence, and circular dichroism spectroscopy. To determine the mechanism, we performed dye-displacement tests, melting temperature investigations, and iodide quenching. RED fluorescence, high-affinity DNA intercalation, aggregation-induced emission (AIE), viscosity sensitivity, and pH-dependent colorimetric response make this ligand a promising candidate for DNA-targeted imaging and pH-sensitive biosensing in biological and environmental applications. Plant cell tissue imaging has also proved successful.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"47 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143258622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We introduce an innovative two-phase-perovskite-based sensing platform for the ultrasensitive detection of capsaicin via the photo-induced electron transfer mechanism, with the limit of detection as low as 0.21 nM. Importantly, the successful use of this probe for monitoring capsaicin in real samples suggests its potential utilization prospects in food sectors.
{"title":"PET-based aqueous-stable two-phase perovskite nanoprobe for quantization of capsaicin in food sample","authors":"Wen-Juan Wang, Kai-Qi Liu, Xiangying Meng, Ze-Yu Sun, Feng Li, Zhong-Xia Wang","doi":"10.1039/d4an01569j","DOIUrl":"https://doi.org/10.1039/d4an01569j","url":null,"abstract":"We introduce an innovative two-phase-perovskite-based sensing platform for the ultrasensitive detection of capsaicin via the photo-induced electron transfer mechanism, with the limit of detection as low as 0.21 nM. Importantly, the successful use of this probe for monitoring capsaicin in real samples suggests its potential utilization prospects in food sectors.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"31 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143258732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shahzad Ahmed, Arshiya Ansari, Bibekananda De, Subrata Mukherjee, Devendra Singh Negi and Pranay Ranjan
Caffeine is a natural stimulant found in various plants. Some individuals are particularly sensitive to caffeine and may experience adverse effects even with minimal intake. In order to address the potential health risks associated with high caffeine use, it is imperative to establish a precise, straightforward, efficient, and cost-efficient approach for measuring caffeine levels in regularly consumed items. This article explores electrochemical techniques for monitoring bitterness induced by caffeine. The fabricated bio-electronic tongue (Bio-ET) comprised a modified electrode made of borophene/PPy@ITO, created by electropolymerizing polypyrrole (PPy) onto indium tin oxide (ITO) and subsequently decorating it with borophene sheets. Cyclic voltammetry (CV) was used to investigate the electrochemical characteristics of caffeine on borophene/PPy@ITO. The findings revealed that the Bio-ET exhibited strong electro-oxidation and reduction activity towards caffeine, indicated by the presence of distinct redox peaks. The Bio-ET demonstrated a linear range from 0.5 to 700 μM with a limit of detection (LOD) of 0.177 μM. The Bio-ET electrode was successfully employed for caffeine quantification in real samples, including coffee, black tea, and regular tea, yielding excellent electrocatalytic performance. Furthermore, the potential of the Bio-ET system could lead to the development of portable, user-friendly devices for on-site analysis, facilitating rapid testing in various settings, such as beverages and pharmaceuticals, and presenting a promising direction for both research and commercial applications.
{"title":"An electrochemical bio-electronic tongue based on borophene/PPy@ITO hybrid for selective caffeine identification†","authors":"Shahzad Ahmed, Arshiya Ansari, Bibekananda De, Subrata Mukherjee, Devendra Singh Negi and Pranay Ranjan","doi":"10.1039/D4AN01547A","DOIUrl":"10.1039/D4AN01547A","url":null,"abstract":"<p >Caffeine is a natural stimulant found in various plants. Some individuals are particularly sensitive to caffeine and may experience adverse effects even with minimal intake. In order to address the potential health risks associated with high caffeine use, it is imperative to establish a precise, straightforward, efficient, and cost-efficient approach for measuring caffeine levels in regularly consumed items. This article explores electrochemical techniques for monitoring bitterness induced by caffeine. The fabricated bio-electronic tongue (Bio-ET) comprised a modified electrode made of borophene/PPy@ITO, created by electropolymerizing polypyrrole (PPy) onto indium tin oxide (ITO) and subsequently decorating it with borophene sheets. Cyclic voltammetry (CV) was used to investigate the electrochemical characteristics of caffeine on borophene/PPy@ITO. The findings revealed that the Bio-ET exhibited strong electro-oxidation and reduction activity towards caffeine, indicated by the presence of distinct redox peaks. The Bio-ET demonstrated a linear range from 0.5 to 700 μM with a limit of detection (LOD) of 0.177 μM. The Bio-ET electrode was successfully employed for caffeine quantification in real samples, including coffee, black tea, and regular tea, yielding excellent electrocatalytic performance. Furthermore, the potential of the Bio-ET system could lead to the development of portable, user-friendly devices for on-site analysis, facilitating rapid testing in various settings, such as beverages and pharmaceuticals, and presenting a promising direction for both research and commercial applications.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 5","pages":" 962-974"},"PeriodicalIF":3.6,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143258621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Metal nanoparticles are commonly found in our daily lives and pose great risks to people's health. Therefore, it is crucial to establish a research model for the toxic effects of metal nanoparticles. In recent decades, three-dimensional (3D) cell models have attracted increasing interest in the fields of cell barriers, nanotoxicology, and drug screening, as they have significant advantages over two-dimensional (2D) cell models in accurately simulating in vivo behavior of human cells. The accurate spatiotemporal reaction characteristics achieved through the diffusion effect of metal nanoparticles in Matrigel scaffolds are of great importance in nanotoxicology. However, traditional impedance sensors face challenges in performing spatiotemporal dynamic impedance monitoring and evaluating the toxic impact of metal nanoparticles on 3D cells. Here, we propose an impedance sensor that integrates a plug-in vertical electrode array (PVEA) chip with a multi-channel detection system. This sensor can dynamically record 3D cell impedance in the vertical direction, which is consistent with the temporal and spatial progression of metal nanoparticle penetration, and also closely related to the spatiotemporal activity of cells influenced by metal nanoparticles. This method can detect subtle changes in impedance signals at different positions caused by the diffusion of metal nanoparticles, and has high application value in Nanotoxicology evaluation. This universal, high-throughput 3D cell impedance sensor has great potential in toxicity detection and drug screening.
{"title":"Spatiotemporal 3D cell impedance monitoring for metal nanoparticle risk assessment by plug-in vertical electrode array","authors":"Yimin Shi, Hui Liu, Mingda Zhao, Sheng Sun, Meiyan Qin, Yang Zhao, Mingxiao Li, Lina Zhang, Lingqian Zhang, Chengjun Huang","doi":"10.1039/d4an01494d","DOIUrl":"https://doi.org/10.1039/d4an01494d","url":null,"abstract":"Metal nanoparticles are commonly found in our daily lives and pose great risks to people's health. Therefore, it is crucial to establish a research model for the toxic effects of metal nanoparticles. In recent decades, three-dimensional (3D) cell models have attracted increasing interest in the fields of cell barriers, nanotoxicology, and drug screening, as they have significant advantages over two-dimensional (2D) cell models in accurately simulating <em>in vivo</em> behavior of human cells. The accurate spatiotemporal reaction characteristics achieved through the diffusion effect of metal nanoparticles in Matrigel scaffolds are of great importance in nanotoxicology. However, traditional impedance sensors face challenges in performing spatiotemporal dynamic impedance monitoring and evaluating the toxic impact of metal nanoparticles on 3D cells. Here, we propose an impedance sensor that integrates a plug-in vertical electrode array (PVEA) chip with a multi-channel detection system. This sensor can dynamically record 3D cell impedance in the vertical direction, which is consistent with the temporal and spatial progression of metal nanoparticle penetration, and also closely related to the spatiotemporal activity of cells influenced by metal nanoparticles. This method can detect subtle changes in impedance signals at different positions caused by the diffusion of metal nanoparticles, and has high application value in Nanotoxicology evaluation. This universal, high-throughput 3D cell impedance sensor has great potential in toxicity detection and drug screening.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"61 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A water-soluble and biologically benign glucose-appended quinoline-benzothiazole conjugate (L) has been synthesized and characterized by various spectroscopy techniques. The recognition properties of L show selective recognition of Cu+ ion among other biological metal ions including Cu2+ ion in PBS buffer at pH 7.4. The L exhibited switch-on fluorescence enhancement after addition of Cu+ with a detection limit of 1.48 × 10‒8 M in an aqueous medium. The job’s plot confirmed the 1:1 binding ratio observed between the probe L and Cu+ ion with a association constant (Ka) of 1.46 × 105 M-1. The proposed complex binding mechanism is supported by UV-Vis, fluorescence and ESI-MS. The coordination features of complex {L+Cu+} have been delineated by DFT computational calculations. Further, the L successfully applied for the intracellular fluorescence bio-imaging of Cu+ ion in L929 living cells, suggesting that this L holds significant potential for Cu+ ion bio-imaging and disease diagnosis.
{"title":"Water soluble glucose-appended quinoline-benzothiazole conjugate as a selective and sensitive receptor for Cu+ ion in aqueous media and intracellular bio-imaging in live cells","authors":"Ravinkumar Valand, Nidhi Pandey, Jayesh Bellare, Sivaiah Areti","doi":"10.1039/d5an00066a","DOIUrl":"https://doi.org/10.1039/d5an00066a","url":null,"abstract":"A water-soluble and biologically benign glucose-appended quinoline-benzothiazole conjugate (L) has been synthesized and characterized by various spectroscopy techniques. The recognition properties of L show selective recognition of Cu+ ion among other biological metal ions including Cu2+ ion in PBS buffer at pH 7.4. The L exhibited switch-on fluorescence enhancement after addition of Cu+ with a detection limit of 1.48 × 10‒8 M in an aqueous medium. The job’s plot confirmed the 1:1 binding ratio observed between the probe L and Cu+ ion with a association constant (Ka) of 1.46 × 105 M-1. The proposed complex binding mechanism is supported by UV-Vis, fluorescence and ESI-MS. The coordination features of complex {L+Cu+} have been delineated by DFT computational calculations. Further, the L successfully applied for the intracellular fluorescence bio-imaging of Cu+ ion in L929 living cells, suggesting that this L holds significant potential for Cu+ ion bio-imaging and disease diagnosis.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"40 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The convergence of smartphones with chemiluminescence and electrochemiluminescence (CL/ECL) assays marks a transformative leap in the realm of sensing technologies. The traditional CL/ECL assays, known for their high sensitivity and versatility, find extensive applications in medical diagnostics, environmental monitoring, food safety, and forensic sciences. However, these techniques have long been constrained due to the requirement of expensive instrumentations and complex reagent handling and hence limited their accessibility to certain environments. In an era where rapid, accurate, and routine analysis is critical, smartphone-enabled CL/ECL systems offer substantial advantages over conventional analytical methods. Leveraging the universal accessibility and technological sophistication of smartphones, the CL/ECL-based sensing combined with the smartphone has evolved into a cost-efficient and accessible analytical platform. The ability of the combined platform to conduct on-site analysis in real-time with minimal effort has emerged as a game-changer, particularly in low-resource settings. This mini-review explores the rapid evolution of smartphone-coupled CL/ECL systems over the last five years. The article covers the areas where the combined platform has been implemented in recent years for various sensing applications. The review further identifies key challenges that are associated with such combined platforms and finally highlights the future perspectives of the present topic.
{"title":"Present and Future of Smartphone-Coupled Chemiluminescence and Electrochemiluminescence Assays: A Mini-Review","authors":"Chunuranjan Dutta, Daniel Citterio, Pabitra Nath","doi":"10.1039/d4an01438c","DOIUrl":"https://doi.org/10.1039/d4an01438c","url":null,"abstract":"The convergence of smartphones with chemiluminescence and electrochemiluminescence (CL/ECL) assays marks a transformative leap in the realm of sensing technologies. The traditional CL/ECL assays, known for their high sensitivity and versatility, find extensive applications in medical diagnostics, environmental monitoring, food safety, and forensic sciences. However, these techniques have long been constrained due to the requirement of expensive instrumentations and complex reagent handling and hence limited their accessibility to certain environments. In an era where rapid, accurate, and routine analysis is critical, smartphone-enabled CL/ECL systems offer substantial advantages over conventional analytical methods. Leveraging the universal accessibility and technological sophistication of smartphones, the CL/ECL-based sensing combined with the smartphone has evolved into a cost-efficient and accessible analytical platform. The ability of the combined platform to conduct on-site analysis in real-time with minimal effort has emerged as a game-changer, particularly in low-resource settings. This mini-review explores the rapid evolution of smartphone-coupled CL/ECL systems over the last five years. The article covers the areas where the combined platform has been implemented in recent years for various sensing applications. The review further identifies key challenges that are associated with such combined platforms and finally highlights the future perspectives of the present topic.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"132 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jie Wang, Yuhui Yi, Mu Yang, Dongyan Tian, Jinshan Liu, Songmei Wu, Yu Yu, Kejian Ding
A highly stable electrochemiluminescent (ECL) sensor based on graphitic carbon nitride (GCN)/Pb2+/polypyrrole (PPy) was developed to detect trace copper ions. The hydrochloric acid immersion technique was applied to rearrange PPy chains for the first time. Under optimal experimental conditions, the ECL intensity increased linearly with the Cu2+ concentration in solution from 10-12 M to 10-6 M (1 pM to 1 uM), with a super low limit of detection (LOD) as 10-12 M (1 pM) (S/N = 3). Additionally, the impact of detection on the localized states of the composite nanomaterial was revealed by applying Vienna ab-initio simulation package (VASP) and Gaussian 16.
{"title":"Construction and mechanism analysis of an ultra-sensitive GCN/Pb2+/PPy heterojunction electrochemiluminescent sensor for detecting Cu (Ⅱ)","authors":"Jie Wang, Yuhui Yi, Mu Yang, Dongyan Tian, Jinshan Liu, Songmei Wu, Yu Yu, Kejian Ding","doi":"10.1039/d4an01564a","DOIUrl":"https://doi.org/10.1039/d4an01564a","url":null,"abstract":"A highly stable electrochemiluminescent (ECL) sensor based on graphitic carbon nitride (GCN)/Pb2+/polypyrrole (PPy) was developed to detect trace copper ions. The hydrochloric acid immersion technique was applied to rearrange PPy chains for the first time. Under optimal experimental conditions, the ECL intensity increased linearly with the Cu2+ concentration in solution from 10-12 M to 10-6 M (1 pM to 1 uM), with a super low limit of detection (LOD) as 10-12 M (1 pM) (S/N = 3). Additionally, the impact of detection on the localized states of the composite nanomaterial was revealed by applying Vienna ab-initio simulation package (VASP) and Gaussian 16.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Robyn A Snow, Paul Steven Simone Jr., Gary Emmert, Michael A Brown
Fluorescence methods have distinct advantages over traditional absorbance methods including greater sensitivity, improved detection limits, and selectivity. Unfortunately, the cost of typical, commercially available fluorescence detectors is beyond what some industrial and research labs can afford or maintain. Having a relatively low-cost, simple to use, and high-sensitivity fluorescence detector would be very beneficial. The aim of this research is to develop a 3D printed flow through fluorescence detector that does not require complex optics or an expensive excitation source and has comparable performance to a commercial detector. The detector presented here was designed to work with nicotinamide-based methods developed in previous research; however, by simply changing the excitation and emission filters this detector can be adapted to other applications. Several evaluation studies were performed where the relative signal-to-noise ratio, detection limits, accuracy, and precision results for the 3D printed detector were compared to those of a commercial detector using nicotinamide as the analyte. Overall, the detector performed comparably or better than a commercial detector for these metrics.
{"title":"A low-cost, high-sensitivity 3D printed fluorescence detector","authors":"Robyn A Snow, Paul Steven Simone Jr., Gary Emmert, Michael A Brown","doi":"10.1039/d4an01378f","DOIUrl":"https://doi.org/10.1039/d4an01378f","url":null,"abstract":"Fluorescence methods have distinct advantages over traditional absorbance methods including greater sensitivity, improved detection limits, and selectivity. Unfortunately, the cost of typical, commercially available fluorescence detectors is beyond what some industrial and research labs can afford or maintain. Having a relatively low-cost, simple to use, and high-sensitivity fluorescence detector would be very beneficial. The aim of this research is to develop a 3D printed flow through fluorescence detector that does not require complex optics or an expensive excitation source and has comparable performance to a commercial detector. The detector presented here was designed to work with nicotinamide-based methods developed in previous research; however, by simply changing the excitation and emission filters this detector can be adapted to other applications. Several evaluation studies were performed where the relative signal-to-noise ratio, detection limits, accuracy, and precision results for the 3D printed detector were compared to those of a commercial detector using nicotinamide as the analyte. Overall, the detector performed comparably or better than a commercial detector for these metrics.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"76 2 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Electrochemical biosensors are frequently employed to identify harmful microbes and disease indicators. However, their practical applicability is constrained by poor signal amplification efficiency and immobilization processes on the probe surface. To get over these restrictions, we here integrated roll-cycling amplification (RCA) and CRISPR/Cas12a gene editing tools with electrochemical biosensors. We constructed an electrochemical biosensor based on RCA to activate the cleavage activity of Cas12a. First, by double-stranded nucleic acid aptamer (ds Apt) specifically binding to E.coli-2571, Apt-b was competitively isolated to bind to T4 ligase and produce circular DNA. This in turn activates RCA, which in turn activates the accessory cleavage activity of CRISPR/Cas12a, which in turn cleaves DNA sequences loaded onto the electrode, changing electrochemical signals. With a linear range of 1×102-1×107 CFU/mL, a detection limit of 5.28 CFU/mL, and experimental recoveries of 93.01-101.53%, the measured electrochemical signals were positively connected with the concentration of E.coli-2571 under the optimal experimental conditions. Therefore, by combining two approaches—RCA and CRISPR/Cas12a—our electrochemical biosensor was able to detect E.coli-2571 specifically and sensitively, opening up new research opportunities for the detection of other harmful bacteria.
{"title":"Electrochemical Biosensor Utilizing CRISPR/Cas12a Amplification for the Detection of E.coli","authors":"Chenyan Li, Yilan Liang, Qingcong Feng","doi":"10.1039/d4an01441c","DOIUrl":"https://doi.org/10.1039/d4an01441c","url":null,"abstract":"Electrochemical biosensors are frequently employed to identify harmful microbes and disease indicators. However, their practical applicability is constrained by poor signal amplification efficiency and immobilization processes on the probe surface. To get over these restrictions, we here integrated roll-cycling amplification (RCA) and CRISPR/Cas12a gene editing tools with electrochemical biosensors. We constructed an electrochemical biosensor based on RCA to activate the cleavage activity of Cas12a. First, by double-stranded nucleic acid aptamer (ds Apt) specifically binding to E.coli-2571, Apt-b was competitively isolated to bind to T4 ligase and produce circular DNA. This in turn activates RCA, which in turn activates the accessory cleavage activity of CRISPR/Cas12a, which in turn cleaves DNA sequences loaded onto the electrode, changing electrochemical signals. With a linear range of 1×102-1×107 CFU/mL, a detection limit of 5.28 CFU/mL, and experimental recoveries of 93.01-101.53%, the measured electrochemical signals were positively connected with the concentration of E.coli-2571 under the optimal experimental conditions. Therefore, by combining two approaches—RCA and CRISPR/Cas12a—our electrochemical biosensor was able to detect E.coli-2571 specifically and sensitively, opening up new research opportunities for the detection of other harmful bacteria.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"23 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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