The reconstruction of critical-size calvarial defects remains a fundamental challenge. Recombinant collagen has gained significant attention in bone tissue engineering owing to its remarkable bioactivity and non-immunogenicity. Herein, we have for the first time developed a bioactive poly(ethylene glycol)-chondroitin sulfate-triple helical recombinant collagen (PEG-ChS-THRC) hydrogel for enhanced bone regeneration in cranial defects. A simple and mild crosslinking reaction of two-arm polyethylene glycol active ester (NHS-PEG-NHS), adipic dihydrazide modified chondroitin sulfate (ChS-ADH) and triple helical recombinant collagen (THRC) leads to the formation of the PEG-ChS-THRC hydrogel. The hydrogel demonstrates interconnected porous structures, enhanced mechanical strength, diminished swelling ratios and adjustable biodegradability. It possesses exceptional biocompatibility and bioactivity, significantly facilitating cell proliferation, adhesion, migration, and osteogenic differentiation of BMSCs. Micro-computed tomography (micro-CT), magnetic resonance imaging (MRI) and histological characterization of rat models with critical-size cranial defects have consistently demonstrated that the PEG-ChS-THRC hydrogel significantly promotes bone tissues regeneration. The innovative bioactive scaffold provides a remarkably improved remedy for critical-size cranial defects, holding greatly promising applications in the fields of bone tissue regeneration.
{"title":"Bioactive poly(ethylene glycol)-chondroitin sulfate-triple helical recombinant collagen hydrogel for enhanced cranial defect repair","authors":"Lili Wang, Shanshan Zhang, Fan Yang, Xian Chen, Huixia He, Zaiman Liu, Jianxi Xiao","doi":"10.1186/s42825-024-00168-4","DOIUrl":"10.1186/s42825-024-00168-4","url":null,"abstract":"<div><p>The reconstruction of critical-size calvarial defects remains a fundamental challenge. Recombinant collagen has gained significant attention in bone tissue engineering owing to its remarkable bioactivity and non-immunogenicity. Herein, we have for the first time developed a bioactive poly(ethylene glycol)-chondroitin sulfate-triple helical recombinant collagen (PEG-ChS-THRC) hydrogel for enhanced bone regeneration in cranial defects. A simple and mild crosslinking reaction of two-arm polyethylene glycol active ester (NHS-PEG-NHS), adipic dihydrazide modified chondroitin sulfate (ChS-ADH) and triple helical recombinant collagen (THRC) leads to the formation of the PEG-ChS-THRC hydrogel. The hydrogel demonstrates interconnected porous structures, enhanced mechanical strength, diminished swelling ratios and adjustable biodegradability. It possesses exceptional biocompatibility and bioactivity, significantly facilitating cell proliferation, adhesion, migration, and osteogenic differentiation of BMSCs. Micro-computed tomography (micro-CT), magnetic resonance imaging (MRI) and histological characterization of rat models with critical-size cranial defects have consistently demonstrated that the PEG-ChS-THRC hydrogel significantly promotes bone tissues regeneration. The innovative bioactive scaffold provides a remarkably improved remedy for critical-size cranial defects, holding greatly promising applications in the fields of bone tissue regeneration.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-024-00168-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141886305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-03DOI: 10.1186/s42825-024-00163-9
Yudan Yi, Xinxin Fan, Qijun Li, Ya-nan Wang
Hydrophobicity enhancement of metal-free leather, which is crucial for improving its comprehensive performance, can be achieved by using amphiphilic copolymer retanning agents. However, the relationship between the sequential structure and the hydrophobic modification effect of amphiphilic copolymers remains unclear. Herein, an amphiphilic block copolymer was synthesized using stearyl methacrylate and 2-(dimethylamino)ethyl methacrylate via atom transfer radical polymerization, and the corresponding random copolymer with similar monomer compositions and molecular weights was prepared for comparison. The aggregation behavior of block and random copolymers was investigated. DLS and TEM results indicate that the block copolymer exhibits a larger aggregate size than the corresponding random copolymer. Molecular dynamics simulations suggest that the block copolymer aggregate exhibit a thicker hydrophilic shell and more concentrated distribution of cationic DMA block than the random copolymer aggregate. Subsequently, the block and random copolymers were used for the hydrophobic modification of metal-free tanned collagen fibers (CFs). The block copolymer shows superior binding capacity to CFs than the random one because of its larger size and more concentrated charge distribution. Hence, the block copolymer can form a dense and uniform hydrophobic film on the surface of collagen fibrils and endow CFs with higher hydrophobicity than the random one. This work provides theoretical guidance for modulating the hydrophobicity of CFs by tailoring the sequential structure of amphiphilic copolymers, which is expected to inspire the manufacturing of high-performance metal-free leather.
Graphical Abstract
使用两亲共聚物复鞣剂可以增强无金属皮革的疏水性，这对提高其综合性能至关重要。然而，两亲共聚物的序列结构与疏水改性效果之间的关系仍不清楚。本文利用甲基丙烯酸硬脂酯和甲基丙烯酸 2-（二甲基氨基）乙酯通过原子转移自由基聚合合成了一种两亲性嵌段共聚物，并制备了单体组成和分子量相似的相应无规共聚物进行比较。研究了嵌段共聚物和无规共聚物的聚集行为。DLS 和 TEM 结果表明，嵌段共聚物的聚集尺寸大于相应的无规共聚物。分子动力学模拟表明，与无规共聚物相比，嵌段共聚物聚集体的亲水外壳更厚，阳离子 DMA 嵌段分布更集中。随后，将嵌段共聚物和无规共聚物用于无金属鞣制胶原纤维（CF）的疏水改性。与无规共聚物相比，嵌段共聚物的尺寸更大，电荷分布更集中，因此与胶原纤维的结合能力更强。因此，嵌段共聚物能在胶原纤维表面形成致密、均匀的疏水膜，并赋予胶原纤维比无规共聚物更高的疏水性。这项工作为通过定制两亲共聚物的序列结构来调节 CF 的疏水性提供了理论指导，有望为高性能无金属皮革的制造带来启发。
{"title":"Amphiphilic block and random copolymers: aggregation and hydrophobic modification on metal-free tanned collagen fibers","authors":"Yudan Yi, Xinxin Fan, Qijun Li, Ya-nan Wang","doi":"10.1186/s42825-024-00163-9","DOIUrl":"10.1186/s42825-024-00163-9","url":null,"abstract":"<div><p>Hydrophobicity enhancement of metal-free leather, which is crucial for improving its comprehensive performance, can be achieved by using amphiphilic copolymer retanning agents. However, the relationship between the sequential structure and the hydrophobic modification effect of amphiphilic copolymers remains unclear. Herein, an amphiphilic block copolymer was synthesized using stearyl methacrylate and 2-(dimethylamino)ethyl methacrylate via atom transfer radical polymerization, and the corresponding random copolymer with similar monomer compositions and molecular weights was prepared for comparison. The aggregation behavior of block and random copolymers was investigated. DLS and TEM results indicate that the block copolymer exhibits a larger aggregate size than the corresponding random copolymer. Molecular dynamics simulations suggest that the block copolymer aggregate exhibit a thicker hydrophilic shell and more concentrated distribution of cationic DMA block than the random copolymer aggregate. Subsequently, the block and random copolymers were used for the hydrophobic modification of metal-free tanned collagen fibers (CFs). The block copolymer shows superior binding capacity to CFs than the random one because of its larger size and more concentrated charge distribution. Hence, the block copolymer can form a dense and uniform hydrophobic film on the surface of collagen fibrils and endow CFs with higher hydrophobicity than the random one. This work provides theoretical guidance for modulating the hydrophobicity of CFs by tailoring the sequential structure of amphiphilic copolymers, which is expected to inspire the manufacturing of high-performance metal-free leather.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-024-00163-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141495484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-02DOI: 10.1186/s42825-024-00161-x
Xiaomin Luo, Lufeng Ji, Fen Ao, Chen Yang, Jiang Chang, Changyu Yin, Huijun Ren, Ming Teng, Liuying Li, Xinhua Liu
Silicate bioceramics have demonstrated great potential in hydrogel dressings for wound healing due to their special origins of promoting endothelial cell angiogenesis and inhibiting apoptosis of cardiomyocyte. However, there are still some deficiencies, such as insufficient biological activity, instability of silicate ion release, and lower wet adhesion on wounds with tissue exudate, limiting their further clinical applications. Herein, inspired by mussels, a multifunctional double-network hydrogel (FS/PAM-Gel-PDA) wound dressing composited gelatin with silicate ceramic powder with satisfactory wet adhesion, stable release of bioactive ions, hemostasis, and the ability of promoting vascular regeneration was engineered through specifically grafting dopamine to gelatin and introducing ferrous silicate ceramic powder into the hydrogel. The comprehensive experimental results substantiate that the FS/PAM-Gel-PDA has wet-adhesion strength of up to 21.78 kPa, and remains stably adherent to porcine myocardial tissues intuitively after bending, twisting, soaking in water, and stretching. The test results of ion release behavior in vitro show that the oxidation and agglomeration of ferrous silicate ceramic powder can be effectively inhibited by using dopamine to form an antioxidant layer on the surface of ceramic powder, and thus, the stable release of Fe2+ and SiO44− effective ions can be realized. The animal experiment exhibits that FS/PAM-Gel-PDA can achieve rapid hemostasis in the lethal liver defect model. Meanwhile, the FS/PAM-Gel-PDA reveals the remarkable ability to promote wound healing in a full-thickness skin injury model, which can obviously accelerate skin re-epithelialization. To sum up, the FS/PAM-Gel-PDA has excellent wet adhesion and stable release of active ions to accelerate angiogenesis, which shows great potential in promoting wound healing.
{"title":"On-demand engineered double-network gelatin/silicate composited hydrogels with enhanced wet adhesion and stable release of bioactive ion for promoting wound healing","authors":"Xiaomin Luo, Lufeng Ji, Fen Ao, Chen Yang, Jiang Chang, Changyu Yin, Huijun Ren, Ming Teng, Liuying Li, Xinhua Liu","doi":"10.1186/s42825-024-00161-x","DOIUrl":"10.1186/s42825-024-00161-x","url":null,"abstract":"<div><p>Silicate bioceramics have demonstrated great potential in hydrogel dressings for wound healing due to their special origins of promoting endothelial cell angiogenesis and inhibiting apoptosis of cardiomyocyte. However, there are still some deficiencies, such as insufficient biological activity, instability of silicate ion release, and lower wet adhesion on wounds with tissue exudate, limiting their further clinical applications. Herein, inspired by mussels, a multifunctional double-network hydrogel (FS/PAM-Gel-PDA) wound dressing composited gelatin with silicate ceramic powder with satisfactory wet adhesion, stable release of bioactive ions, hemostasis, and the ability of promoting vascular regeneration was engineered through specifically grafting dopamine to gelatin and introducing ferrous silicate ceramic powder into the hydrogel. The comprehensive experimental results substantiate that the FS/PAM-Gel-PDA has wet-adhesion strength of up to 21.78 kPa, and remains stably adherent to porcine myocardial tissues intuitively after bending, twisting, soaking in water, and stretching. The test results of ion release behavior in vitro show that the oxidation and agglomeration of ferrous silicate ceramic powder can be effectively inhibited by using dopamine to form an antioxidant layer on the surface of ceramic powder, and thus, the stable release of Fe<sup>2+</sup> and SiO<sub>4</sub>\u0000<sup>4−</sup> effective ions can be realized. The animal experiment exhibits that FS/PAM-Gel-PDA can achieve rapid hemostasis in the lethal liver defect model. Meanwhile, the FS/PAM-Gel-PDA reveals the remarkable ability to promote wound healing in a full-thickness skin injury model, which can obviously accelerate skin re-epithelialization. To sum up, the FS/PAM-Gel-PDA has excellent wet adhesion and stable release of active ions to accelerate angiogenesis, which shows great potential in promoting wound healing.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-024-00161-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141495495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acellular dermal matrix (ADM) is one of the most promising scaffold materials due to its ability to retain natural extracellular matrix structure. Micronized acellular dermal matrix (mADM) was prepared with no intact cell nuclei and preserved growth factors by High Hydrostatic Pressure (HHP) approach. And mADM-collagen wound dressings were developed with different proportion of type I collagen and recombinant humanized type III collagen. The porous structure of the mADM-collagen wound dressings made them a good candidate for preventing excessive fluid accumulation, while the collagens with gel-like texture combined with mADM powder to form pasty texture wound dressing, which preserving the moisture at the wound site. Moreover, the paste texture of the mADM-collagen wound dressing was easy to reshape to conform any wound shapes and body contours. Furthermore, the resulted mADM-collagen wound dressings showed good biocompatibility by supporting fibroblasts adhesion and proliferation in vitro. Subsequently, a murine model of full-thickness skin wounds was employed to assess its effects on wound healing. Notably, mADM-75% Col-I exhibited superior effects throughout the wound healing process, specifically it promoted neovascularization, skin appendage growth and new skin regeneration. This formulation closely mimicked the collagen ratio found in healthy skin, facilitating the favorable wound repair. These results indicated the superior performance of this mADM-collagen wound dressing providing an optimal environment for wound healing.
Graphical Abstract
细胞外基质（ADM）能够保留天然细胞外基质结构，是最有前途的支架材料之一。通过高静水压（HHP）方法制备的微粉化细胞外基质（mADM）没有完整的细胞核，并保留了生长因子。并使用不同比例的 I 型胶原蛋白和重组人源化 III 型胶原蛋白开发了 mADM-胶原蛋白伤口敷料。mADM 胶原伤口敷料的多孔结构使其成为防止液体过度积聚的理想选择，而具有凝胶质地的胶原蛋白与 mADM 粉末结合形成糊状质地的伤口敷料，可保持伤口部位的湿度。此外，mADM-胶原蛋白伤口敷料的糊状质地易于重塑，以适应任何伤口形状和身体轮廓。此外，制成的 mADM-胶原伤口敷料在体外支持成纤维细胞的粘附和增殖，表现出良好的生物相容性。随后，我们利用小鼠全厚皮肤伤口模型来评估其对伤口愈合的影响。值得注意的是，mADM-75% Col-I 在整个伤口愈合过程中表现出卓越的效果，特别是它能促进血管新生、皮肤附属物生长和新皮肤再生。这种配方非常接近健康皮肤中的胶原蛋白比例，有利于伤口修复。这些结果表明，这种 mADM 胶原伤口敷料性能优越，可为伤口愈合提供最佳环境。
{"title":"Development of mADM-collagen wound dressings for mimicking native skin architecture to enhance skin wound healing","authors":"Xiang Wang, Yujia Jiang, Xiaoqin Sun, Chongxia Yue, Zhengyong Li, Yao Wu","doi":"10.1186/s42825-024-00159-5","DOIUrl":"10.1186/s42825-024-00159-5","url":null,"abstract":"<div><p>Acellular dermal matrix (ADM) is one of the most promising scaffold materials due to its ability to retain natural extracellular matrix structure. Micronized acellular dermal matrix (mADM) was prepared with no intact cell nuclei and preserved growth factors by High Hydrostatic Pressure (HHP) approach. And mADM-collagen wound dressings were developed with different proportion of type I collagen and recombinant humanized type III collagen. The porous structure of the mADM-collagen wound dressings made them a good candidate for preventing excessive fluid accumulation, while the collagens with gel-like texture combined with mADM powder to form pasty texture wound dressing, which preserving the moisture at the wound site. Moreover, the paste texture of the mADM-collagen wound dressing was easy to reshape to conform any wound shapes and body contours. Furthermore, the resulted mADM-collagen wound dressings showed good biocompatibility by supporting fibroblasts adhesion and proliferation in vitro. Subsequently, a murine model of full-thickness skin wounds was employed to assess its effects on wound healing. Notably, mADM-75% Col-I exhibited superior effects throughout the wound healing process, specifically it promoted neovascularization, skin appendage growth and new skin regeneration. This formulation closely mimicked the collagen ratio found in healthy skin, facilitating the favorable wound repair. These results indicated the superior performance of this mADM-collagen wound dressing providing an optimal environment for wound healing.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-024-00159-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141475044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-02DOI: 10.1186/s42825-024-00162-w
Fetra J. Andriamanohiarisoamanana, Mohamed Farghali, Israa M. A. Mohamed, Gen Yoshida, Kazuya Shiota, Ikko Ihara
The study investigates the potential of anaerobic co-digestion (AcoD) as a sustainable solution for managing putrescible organic waste generated by leather processing. Three experiments were conducted to assess the impact of various tannery wastes, pretreatment methods, and waste combinations on methane production. Experiment 1 demonstrated that co-digesting tannery wastewater primary sludge (TWPS) and fleshings significantly increased methane yield compared to digesting TWPS alone, though the addition of chromium- and vegetable-tanned leather wastes decreased yield. Experiment 2 explored TWPS pretreatment methods and found that ultrasonic pretreatment increased soluble chemical oxygen demand (SCOD) but did not significantly improve methane yield, suggesting that pretreatment may not be necessary. Experiment 3 revealed that increasing the proportion of fleshings to TWPS resulted in higher methane yield, ranging from 226.52 mL/gVS with 6% fleshings to 395.71 mL/gVS and 538.34 mL/gVS with 12% and 20% of fleshings, respectively. Additionally, this increase in fleshings also led to a reduction in digester volume. These findings highlight the importance of AcoD in addressing both environmental and economic challenges in the leather industry.
{"title":"Impact of tannery wastes on anaerobic co-digestion: enhancing biogas production and process efficiency","authors":"Fetra J. Andriamanohiarisoamanana, Mohamed Farghali, Israa M. A. Mohamed, Gen Yoshida, Kazuya Shiota, Ikko Ihara","doi":"10.1186/s42825-024-00162-w","DOIUrl":"10.1186/s42825-024-00162-w","url":null,"abstract":"<div><p>The study investigates the potential of anaerobic co-digestion (AcoD) as a sustainable solution for managing putrescible organic waste generated by leather processing. Three experiments were conducted to assess the impact of various tannery wastes, pretreatment methods, and waste combinations on methane production. Experiment 1 demonstrated that co-digesting tannery wastewater primary sludge (TWPS) and fleshings significantly increased methane yield compared to digesting TWPS alone, though the addition of chromium- and vegetable-tanned leather wastes decreased yield. Experiment 2 explored TWPS pretreatment methods and found that ultrasonic pretreatment increased soluble chemical oxygen demand (SCOD) but did not significantly improve methane yield, suggesting that pretreatment may not be necessary. Experiment 3 revealed that increasing the proportion of fleshings to TWPS resulted in higher methane yield, ranging from 226.52 mL/gVS with 6% fleshings to 395.71 mL/gVS and 538.34 mL/gVS with 12% and 20% of fleshings, respectively. Additionally, this increase in fleshings also led to a reduction in digester volume. These findings highlight the importance of AcoD in addressing both environmental and economic challenges in the leather industry.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-024-00162-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141187286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01DOI: 10.1186/s42825-024-00158-6
Siyan Deng, Hongfu Cao, Yan Lu, Wenqing Shi, Manyu Chen, Xiaolin Cui, Jie Liang, Yujiang Fan, Qiguang Wang, Xingdong Zhang
The interior environment of articular cartilage in osteoarthritis (OA) presents substantial hurdles, leading to the malfunction of chondrocytes and the breakdown of collagen II-enriched hyaline cartilage matrix. Despite this, most clinical treatments primarily provide temporary relief from OA discomfort without arresting OA progression. This study aimed to alleviate OA by developing intra-articular injectable dECM-enhanced hyaluronic (HE) microgels. The HE hydrogel was engineered and shaped into uniformly sized microgels using microfluidics and photopolymerization techniques. These microgels provided a spatiotemporal cascade effect, facilitating the rapid release of growth factors and a slower release of ECM macromolecules and proteins. This process assisted in the recovery of OA chondrocytes’ function, promoting cell proliferation, matrix synthesis, and cartilage-specific gene expression in vitro. It also effectively aided repair of the collagen II-enriched hyaline cartilage and significantly reduced the severity of OA, as demonstrated by radiological observation, gross appearance, histological/immunohistochemical staining, and analysis in an OA rat model in vivo. Collectively, the HE injectable microgels with spatiotemporal release of cartilage-specific molecules have shown promise as a potential candidate for a cell-free OA therapy approach.
Graphical Abstract
骨关节炎（OA）的关节软骨内部环境存在巨大障碍，导致软骨细胞功能失调和富含胶原蛋白 II 的透明软骨基质分解。尽管如此，大多数临床治疗方法只能暂时缓解 OA 带来的不适，却无法阻止 OA 的发展。本研究旨在通过开发可关节内注射的 dECM 增强透明质酸（HE）微凝胶来缓解 OA。利用微流体技术和光聚合技术，将透明质酸水凝胶设计和成型为大小一致的微凝胶。这些微凝胶产生了时空级联效应，促进了生长因子的快速释放以及 ECM 大分子和蛋白质的缓慢释放。这一过程有助于恢复 OA 软骨细胞的功能，促进细胞增殖、基质合成和体外软骨特异性基因表达。在 OA 大鼠模型中进行的放射学观察、大体外观、组织学/免疫组织化学染色和体内分析表明，它还能有效帮助修复富含胶原蛋白 II 的透明软骨，并显著减轻 OA 的严重程度。总之，具有软骨特异性分子时空释放功能的 HE 可注射微凝胶有望成为无细胞 OA 治疗方法的潜在候选材料。
{"title":"Injectable dECM-enhanced hyaluronic microgels with spatiotemporal release of cartilage-specific molecules to improve osteoarthritic chondrocyte’s function","authors":"Siyan Deng, Hongfu Cao, Yan Lu, Wenqing Shi, Manyu Chen, Xiaolin Cui, Jie Liang, Yujiang Fan, Qiguang Wang, Xingdong Zhang","doi":"10.1186/s42825-024-00158-6","DOIUrl":"10.1186/s42825-024-00158-6","url":null,"abstract":"<div><p>The interior environment of articular cartilage in osteoarthritis (OA) presents substantial hurdles, leading to the malfunction of chondrocytes and the breakdown of collagen II-enriched hyaline cartilage matrix. Despite this, most clinical treatments primarily provide temporary relief from OA discomfort without arresting OA progression. This study aimed to alleviate OA by developing intra-articular injectable dECM-enhanced hyaluronic (HE) microgels. The HE hydrogel was engineered and shaped into uniformly sized microgels using microfluidics and photopolymerization techniques. These microgels provided a spatiotemporal cascade effect, facilitating the rapid release of growth factors and a slower release of ECM macromolecules and proteins. This process assisted in the recovery of OA chondrocytes’ function, promoting cell proliferation, matrix synthesis, and cartilage-specific gene expression in vitro. It also effectively aided repair of the collagen II-enriched hyaline cartilage and significantly reduced the severity of OA, as demonstrated by radiological observation, gross appearance, histological/immunohistochemical staining, and analysis in an OA rat model in vivo. Collectively, the HE injectable microgels with spatiotemporal release of cartilage-specific molecules have shown promise as a potential candidate for a cell-free OA therapy approach.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-024-00158-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141187289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01DOI: 10.1186/s42825-024-00165-7
Lu Song, He Qiu, Zhiru Chen, Jing Wang, Yang Xu, Zhanhong Liu, Shuo Liu, Zhiyuan Wang, Xiangdong Zhu, Kai Zhang, Hai Lin, Xingdong Zhang
Collagen, known for its excellent biocompatibility and biological properties, has limited in vivo maintenance duration after implantation, while hyaluronic acid faces challenges such as various complications and insufficient support for cell proliferation. In this study, an injectable hyaluronic acid/collagen (HCol) hydrogel was developed to achieve enhanced cell-material interactions and accelerated skin regeneration. Physical and chemical characterizations demonstrated that the HCol hydrogel was injectable and stable after the implantation. In vitro cell culture results illustrated that the hydrogel promoted the proliferation of human dermal fibroblasts, extracellular matrix expression and angiogenesis. The subcutaneous implantation in rats showed the superior biocompatibility of HCol hydrogel and enhanced secretion and deposition of extracellular matrix, compared with commercial hyaluronic acid dermal filler. MRI analysis showed that the hydrogel stably remained in vivo for at least three months. The histological examination and SHG signals further demonstrated that the hydrogel modulated fibroblast phenotype and stimulated vascular ingrowth and collagen synthesis, without inducing significant inflammation, swelling or erythema in vivo.
{"title":"Injectable hyaluronate/collagen hydrogel with enhanced safety and efficacy for facial rejuvenation","authors":"Lu Song, He Qiu, Zhiru Chen, Jing Wang, Yang Xu, Zhanhong Liu, Shuo Liu, Zhiyuan Wang, Xiangdong Zhu, Kai Zhang, Hai Lin, Xingdong Zhang","doi":"10.1186/s42825-024-00165-7","DOIUrl":"10.1186/s42825-024-00165-7","url":null,"abstract":"<div><p>Collagen, known for its excellent biocompatibility and biological properties, has limited in vivo maintenance duration after implantation, while hyaluronic acid faces challenges such as various complications and insufficient support for cell proliferation. In this study, an injectable hyaluronic acid/collagen (HCol) hydrogel was developed to achieve enhanced cell-material interactions and accelerated skin regeneration. Physical and chemical characterizations demonstrated that the HCol hydrogel was injectable and stable after the implantation. In vitro cell culture results illustrated that the hydrogel promoted the proliferation of human dermal fibroblasts, extracellular matrix expression and angiogenesis. The subcutaneous implantation in rats showed the superior biocompatibility of HCol hydrogel and enhanced secretion and deposition of extracellular matrix, compared with commercial hyaluronic acid dermal filler. MRI analysis showed that the hydrogel stably remained in vivo for at least three months. The histological examination and SHG signals further demonstrated that the hydrogel modulated fibroblast phenotype and stimulated vascular ingrowth and collagen synthesis, without inducing significant inflammation, swelling or erythema in vivo.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-024-00165-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141187288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01DOI: 10.1186/s42825-024-00167-5
Xinyue Zhang, Xue Zhan, Chen Hu, Zuqin Dong, Tao Luo, Haihang Li, Xiaoju Fan, Jie Liang, Yafang Chen, Yujiang Fan
The objective of regenerative wound healing dressings is to accelerate skin tissue regeneration and restore normal physiological function at wound sites. Achieving this goal requires biomaterials capable of repairing distinct phases of wound healing in a way that balances material function, degradation, safety, and tissue growth. In this study, we introduced a novel dual-stage wound dressing system comprising methacrylic anhydride-modified recombinant humanized type III collagen (rhCol III-MA) and methacrylic anhydride-modified dopamine (DMA) (RMDM), which was synthesized through free radical polymerization and π-π stacking. Within this system, RMDM was formulated into two forms with identical compositions: hydrogel and sponge, tailored for application across various stages of wound repair. These materials displayed favorable hemocompatibility, biocompatibility, antioxidant properties, and angiogenic potential in vitro. Moreover, the in vivo experiments also demonstrated that sponges could rapidly stop the bleeding of wounds in mouse tail amputation and liver incision models. Notably, the sponge/gel (S/G) system accelerated wound healing compared to individual sponge and gel treatments in a rat full-thickness skin wound model, underscoring the synergistic benefits of combining sponge and gel materials for wound repair at different stages. Therefore, this research provides valuable insights into designing advanced biomaterials that can be tailored to specific stages of wound healing, which may have significant potential for biomedical applications.
Graphical Abstract
再生伤口愈合敷料的目标是加速皮肤组织再生，恢复伤口部位的正常生理功能。实现这一目标需要生物材料能够修复伤口愈合的不同阶段，同时兼顾材料功能、降解、安全性和组织生长。在这项研究中，我们介绍了一种新型双阶段伤口敷料系统，该系统由甲基丙烯酸酐改性重组人源化 III 型胶原蛋白（rhCol III-MA）和甲基丙烯酸酐改性多巴胺（DMA）（RMDM）组成，后者是通过自由基聚合和 π-π 堆积合成的。在这一系统中，RMDM 被配制成两种具有相同成分的形式：水凝胶和海绵，适用于不同阶段的伤口修复。这些材料在体外显示出良好的血液相容性、生物相容性、抗氧化性和血管生成潜力。此外，体内实验也表明，海绵能在小鼠断尾和肝脏切口模型中迅速止住伤口出血。值得注意的是，在大鼠全厚皮肤伤口模型中，海绵/凝胶（S/G）系统与单独的海绵和凝胶处理相比，可加速伤口愈合，这凸显了海绵和凝胶材料在伤口修复不同阶段的协同作用。因此，这项研究为设计可针对伤口愈合特定阶段的先进生物材料提供了宝贵的见解，这可能在生物医学应用方面具有巨大的潜力。
{"title":"Light-controlled crosslinked multifunctional \"Band-Aids\" as dual-stage wound dressing for dynamic wound closure","authors":"Xinyue Zhang, Xue Zhan, Chen Hu, Zuqin Dong, Tao Luo, Haihang Li, Xiaoju Fan, Jie Liang, Yafang Chen, Yujiang Fan","doi":"10.1186/s42825-024-00167-5","DOIUrl":"10.1186/s42825-024-00167-5","url":null,"abstract":"<div><p>The objective of regenerative wound healing dressings is to accelerate skin tissue regeneration and restore normal physiological function at wound sites. Achieving this goal requires biomaterials capable of repairing distinct phases of wound healing in a way that balances material function, degradation, safety, and tissue growth. In this study, we introduced a novel dual-stage wound dressing system comprising methacrylic anhydride-modified recombinant humanized type III collagen (rhCol III-MA) and methacrylic anhydride-modified dopamine (DMA) (RMDM), which was synthesized through free radical polymerization and π-π stacking. Within this system, RMDM was formulated into two forms with identical compositions: hydrogel and sponge, tailored for application across various stages of wound repair. These materials displayed favorable hemocompatibility, biocompatibility, antioxidant properties, and angiogenic potential <i>in vitro</i>. Moreover, the <i>in vivo</i> experiments also demonstrated that sponges could rapidly stop the bleeding of wounds in mouse tail amputation and liver incision models. Notably, the sponge/gel (S/G) system accelerated wound healing compared to individual sponge and gel treatments in a rat full-thickness skin wound model, underscoring the synergistic benefits of combining sponge and gel materials for wound repair at different stages. Therefore, this research provides valuable insights into designing advanced biomaterials that can be tailored to specific stages of wound healing, which may have significant potential for biomedical applications.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-024-00167-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141187285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-22DOI: 10.1186/s42825-024-00164-8
Lu Zheng, Natalya Tseomashko, Anastasiia Voronova, Alexander Vasil’kov, Xiaoqing Hu, Xiaoying Wang
Collagen possesses high biocompatibility with all tissue and cell types in the body, enabling the creation of multifunctional composite materials for medical applications. In biomedical engineering, naturally-sourced collagen is often combined with diverse organic and inorganic bioactive components to eliminate defects and disorders in fields including orthopedics, dermatology, and more. At the same time, medical-related infection issues and the precise treatment needs of patients require collagen composite biomaterials to have antibacterial properties and customized structures. This paper reviews the antibacterial functionalization of collagen composite biomaterials in recent years, including the combination with inorganic or organic antibacterial agents, which is beneficial for preventing and controlling biological contamination in medical applications. Then, the existing problems and future development directions for the architecturalization of collagen composite materials with 3D printing were discussed, providing guidance for personalized customization of multifunctional materials to meet the specific needs of patients in the future.
{"title":"Recent advances of collagen composite biomaterials for biomedical engineering: antibacterial functionalization and 3D-printed architecturalization","authors":"Lu Zheng, Natalya Tseomashko, Anastasiia Voronova, Alexander Vasil’kov, Xiaoqing Hu, Xiaoying Wang","doi":"10.1186/s42825-024-00164-8","DOIUrl":"10.1186/s42825-024-00164-8","url":null,"abstract":"<div><p>Collagen possesses high biocompatibility with all tissue and cell types in the body, enabling the creation of multifunctional composite materials for medical applications. In biomedical engineering, naturally-sourced collagen is often combined with diverse organic and inorganic bioactive components to eliminate defects and disorders in fields including orthopedics, dermatology, and more. At the same time, medical-related infection issues and the precise treatment needs of patients require collagen composite biomaterials to have antibacterial properties and customized structures. This paper reviews the antibacterial functionalization of collagen composite biomaterials in recent years, including the combination with inorganic or organic antibacterial agents, which is beneficial for preventing and controlling biological contamination in medical applications. Then, the existing problems and future development directions for the architecturalization of collagen composite materials with 3D printing were discussed, providing guidance for personalized customization of multifunctional materials to meet the specific needs of patients in the future.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-024-00164-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141078895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-02DOI: 10.1186/s42825-024-00160-y
Yuhao Zhou, Yuer Zhang, Hongjie Dai, Yuhao Zhang, Yu Fu
The increasing global aging population has led to a continual rise in the prevalence of bone and joint diseases, posing challenges to both the quality of life for patients and healthcare resources. Type II collagen, a pivotal protein for sustaining joint function, has gained substantial attention in recent years. The oral administration of undenatured type II collagen (UC-II) has demonstrated noteworthy advancements in tackling bone and joint diseases. This article presents a comprehensive review of the structure and extraction methods of UC-II, discusses the relationship between UC-II and arthritis, and thoroughly examines its therapeutic role and potential mechanisms in the treatment process. In addition, future perspectives for clinical application of UC-II are discussed. It was found that the oral administration of UC-II, through induction of oral tolerance mechanisms, exhibits promise in alleviating joint inflammation and pain in patients with osteoarthritis (OA) and rheumatoid arthritis (RA). This method can significantly ameliorate joint inflammation and pain, with high patient acceptance and minimal side effects, demonstrating its potential as a well-tolerated treatment option for joint diseases.
{"title":"The potential of undenatured type II collagen against arthritis: a review","authors":"Yuhao Zhou, Yuer Zhang, Hongjie Dai, Yuhao Zhang, Yu Fu","doi":"10.1186/s42825-024-00160-y","DOIUrl":"10.1186/s42825-024-00160-y","url":null,"abstract":"<div><p>The increasing global aging population has led to a continual rise in the prevalence of bone and joint diseases, posing challenges to both the quality of life for patients and healthcare resources. Type II collagen, a pivotal protein for sustaining joint function, has gained substantial attention in recent years. The oral administration of undenatured type II collagen (UC-II) has demonstrated noteworthy advancements in tackling bone and joint diseases. This article presents a comprehensive review of the structure and extraction methods of UC-II, discusses the relationship between UC-II and arthritis, and thoroughly examines its therapeutic role and potential mechanisms in the treatment process. In addition, future perspectives for clinical application of UC-II are discussed. It was found that the oral administration of UC-II, through induction of oral tolerance mechanisms, exhibits promise in alleviating joint inflammation and pain in patients with osteoarthritis (OA) and rheumatoid arthritis (RA). This method can significantly ameliorate joint inflammation and pain, with high patient acceptance and minimal side effects, demonstrating its potential as a well-tolerated treatment option for joint diseases.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-024-00160-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140820447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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