Pub Date : 2023-02-10DOI: 10.1186/s42825-023-00114-w
Xu Peng, Li Li, Jiaqi Xing, Can Cheng, Mengyue Hu, Yihao Luo, Shubin Shi, Yan Liu, Zhihui Cui, Xixun Yu
The use of natural polysaccharide crosslinkers for decellularized matrices is an effective approach to prepare cardiovascular substitute materials. In this research, NaIO4 was applied to oxidize konjac glucomannan to prepare the polysaccharide crosslinker oxidized konjac glucomannan (OKGM). The as-prepared crosslinker was then used to stabilize collagen-rich decellularized porcine peritoneum (DPP) to construct a cardiovascular substitute material (OKGM-fixed DPP). The results demonstrated that compared with GA-fixed DPP and GNP-fixed DPP, 3.75% OKGM [1:1.5 (KGM: NaIO4)]-fixed DPP demonstrated suitable mechanical properties, as well as good hemocompatibility, excellent anti-calcification capability, and anti-enzymolysis in vitro. Furthermore, 3.75% OKGM [1:1.5 (KGM: NaIO4)]-fixed DPP was suitable for vascular endothelial cell adhesion and rapid proliferation, and a single layer of endothelial cells was formed on the fifth day of culture. The in vivo experimental results also showed excellent histocompatibility. The current results demonstrted that OKGM was a novel polysaccharide cross-linking reagent for crosslinking natural tissues featured with rich collagen content, and 3.75% OKGM [1:1.5 (KGM: NaIO4)]-fixed DPP was a potential cardiovascular substitute material.
{"title":"Cross-linking porcine peritoneum by oxidized konjac glucomannan: a novel method to improve the properties of cardiovascular substitute material","authors":"Xu Peng, Li Li, Jiaqi Xing, Can Cheng, Mengyue Hu, Yihao Luo, Shubin Shi, Yan Liu, Zhihui Cui, Xixun Yu","doi":"10.1186/s42825-023-00114-w","DOIUrl":"10.1186/s42825-023-00114-w","url":null,"abstract":"<div><p>The use of natural polysaccharide crosslinkers for decellularized matrices is an effective approach to prepare cardiovascular substitute materials. In this research, NaIO<sub>4</sub> was applied to oxidize konjac glucomannan to prepare the polysaccharide crosslinker oxidized konjac glucomannan (OKGM). The as-prepared crosslinker was then used to stabilize collagen-rich decellularized porcine peritoneum (DPP) to construct a cardiovascular substitute material (OKGM-fixed DPP). The results demonstrated that compared with GA-fixed DPP and GNP-fixed DPP, 3.75% OKGM [1:1.5 (KGM: NaIO<sub>4</sub>)]-fixed DPP demonstrated suitable mechanical properties, as well as good hemocompatibility, excellent anti-calcification capability, and anti-enzymolysis in vitro. Furthermore, 3.75% OKGM [1:1.5 (KGM: NaIO<sub>4</sub>)]-fixed DPP was suitable for vascular endothelial cell adhesion and rapid proliferation, and a single layer of endothelial cells was formed on the fifth day of culture. The in vivo experimental results also showed excellent histocompatibility. The current results demonstrted that OKGM was a novel polysaccharide cross-linking reagent for crosslinking natural tissues featured with rich collagen content, and 3.75% OKGM [1:1.5 (KGM: NaIO<sub>4</sub>)]-fixed DPP was a potential cardiovascular substitute material.</p><h3>Graphical Abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-023-00114-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4417652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.1186/s42825-023-00112-y
Yuanzhang Jiang, Harun Venkatesan, Shuo Shi, Cong Wang, Miao Cui, Qiang Zhang, Lin Tan, Jinlian Hu
Spider-capture-silk (SCS) can directionally capture and transport water from humid air relying on the unique geometrical structure. Although there have been adequate reports on the fabrication of artificial SCSs from petroleum-based materials, it remains a big challenge to innovate bio-based SCS mimicking fibers with high-performance fog collection ability and efficiency simultaneously. Herein, we report an eco-friendly and economical fiber system for water collection by coating gelatin on degummed silk. Compared to the previously reported fibers with the best fog collection ability (~ 13.10 μL), Gelatin on silk fiber 10 (GSF10) can collect larger water droplet (~ 16.70 μL in 330 s) with ~ 98% less mass. Meanwhile, the water collection efficiency of GSF10 demonstrates ~ 72% and ~ 48% enhancement to the existing best water collection polymer coated SCS fibers and spidroin eMaSp2 coated degummed silk respectively in terms of volume-to-TCL (vapor–liquid-solid three-phase contact line) index. The simultaneous function of superhydrophilicity, surface energy gradient, and ~ 65% water-induced volume swelling of the gelatin knots are the key factors in advancing the water collection performance. Abundant availability of feedstocks and ~ 75% improved space utilization guarantee the scalability and practical application of such bio-based fiber.
{"title":"Spider-capture-silk mimicking fibers with high-performance fog collection derived from superhydrophilicity and volume-swelling of gelatin knots","authors":"Yuanzhang Jiang, Harun Venkatesan, Shuo Shi, Cong Wang, Miao Cui, Qiang Zhang, Lin Tan, Jinlian Hu","doi":"10.1186/s42825-023-00112-y","DOIUrl":"10.1186/s42825-023-00112-y","url":null,"abstract":"<div><p>Spider-capture-silk (SCS) can directionally capture and transport water from humid air relying on the unique geometrical structure. Although there have been adequate reports on the fabrication of artificial SCSs from petroleum-based materials, it remains a big challenge to innovate bio-based SCS mimicking fibers with high-performance fog collection ability and efficiency simultaneously. Herein, we report an eco-friendly and economical fiber system for water collection by coating gelatin on degummed silk. Compared to the previously reported fibers with the best fog collection ability (~ 13.10 μL), Gelatin on silk fiber 10 (GSF10) can collect larger water droplet (~ 16.70 μL in 330 s) with ~ 98% less mass. Meanwhile, the water collection efficiency of GSF10 demonstrates ~ 72% and ~ 48% enhancement to the existing best water collection polymer coated SCS fibers and spidroin eMaSp2 coated degummed silk respectively in terms of volume-to-TCL (vapor–liquid-solid three-phase contact line) index. The simultaneous function of superhydrophilicity, surface energy gradient, and ~ 65% water-induced volume swelling of the gelatin knots are the key factors in advancing the water collection performance. Abundant availability of feedstocks and ~ 75% improved space utilization guarantee the scalability and practical application of such bio-based fiber.</p><h3>Graphic Abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-023-00112-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4118481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-30DOI: 10.1186/s42825-023-00111-z
Journal of Leather Science, Engineering
{"title":"Correction: Ethics approval and consent to participate","authors":"Journal of Leather Science, Engineering","doi":"10.1186/s42825-023-00111-z","DOIUrl":"10.1186/s42825-023-00111-z","url":null,"abstract":"","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-023-00111-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4011836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-14DOI: 10.1186/s42825-022-00110-6
Chengkun Zhao, Xing Li, Shaoquan Bian, Weinan Zeng, Alfredo Ronca, Ugo D’Amora, Maria Grazia Raucci, Jie Liang, Yong Sun, Qing Jiang, Yujiang Fan, Luigi Ambrosio, Xingdong Zhang
Supramolecular peptides exhibit obvious similarities with collagen fibers in terms of self-assembly characteristics, nanofibrous structure, and responsiveness to external stimuli. Here, a series of supramolecular peptides were developed by altering the amino acid sequence, enabling the self-assembly of three types of 4-biphenylacetic acid (BPAA)-tripeptides into fibrous hydrogel through hydrogen bonding and π–π stacking under the influence of ion induction. Transmission electron and scanning electron microscopies revealed that the diameter of the fiber within nanofibrous hydrogels was ~ 10 and ~ 40 nm, respectively, which was similar with the self-assembled collagen fibers. For this reason, these hydrogels could be considered as a biomimetic extracellular substitute. Meanwhile, the gelation concentration induced by ions was even lower than 0.66 wt%, with an elastic modulus of ~ 0.27 kPa, corresponding to a water content of 99.34 wt%. In addition, the three supramolecular hydrogels were found to be good substrates for L929 cell adhesion and MC-3T3 cell proliferation. The overall results implied that BPAA-based hydrogels have a lucrative application potential as cell carriers.
{"title":"Nanofibrous polypeptide hydrogels with collagen-like structure as biomimetic extracellular matrix","authors":"Chengkun Zhao, Xing Li, Shaoquan Bian, Weinan Zeng, Alfredo Ronca, Ugo D’Amora, Maria Grazia Raucci, Jie Liang, Yong Sun, Qing Jiang, Yujiang Fan, Luigi Ambrosio, Xingdong Zhang","doi":"10.1186/s42825-022-00110-6","DOIUrl":"10.1186/s42825-022-00110-6","url":null,"abstract":"<div><p>Supramolecular peptides exhibit obvious similarities with collagen fibers in terms of self-assembly characteristics, nanofibrous structure, and responsiveness to external stimuli. Here, a series of supramolecular peptides were developed by altering the amino acid sequence, enabling the self-assembly of three types of 4-biphenylacetic acid (BPAA)-tripeptides into fibrous hydrogel through hydrogen bonding and π–π stacking under the influence of ion induction. Transmission electron and scanning electron microscopies revealed that the diameter of the fiber within nanofibrous hydrogels was ~ 10 and ~ 40 nm, respectively, which was similar with the self-assembled collagen fibers. For this reason, these hydrogels could be considered as a biomimetic extracellular substitute. Meanwhile, the gelation concentration induced by ions was even lower than 0.66 wt%, with an elastic modulus of ~ 0.27 kPa, corresponding to a water content of 99.34 wt%. In addition, the three supramolecular hydrogels were found to be good substrates for L929 cell adhesion and MC-3T3 cell proliferation. The overall results implied that BPAA-based hydrogels have a lucrative application potential as cell carriers.</p><h3>Graphical Abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-022-00110-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4572562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The production of high-valued organonitrogen chemicals, especially N-heterocycles, requires artificial N2 fixation accompanied by the consumption of fossil resources. To avoid the use of these energy- and resource-intensive processes, we develop a sustainable strategy to convert nitrogen-rich animal biomass into N-heterocycles through a thermochemical conversion process (TCP) under atmospheric pressure. A high percentage of N-heterocycles (87.51%) were obtained after the TCP of bovine skin due to the abundance of nitrogen-containing amino acids (e.g., glycine, proline, and l-hydroxyproline). Animal biomass with more diverse amino acid composition (e.g., muscles) yielded higher concentrations of amines/amides and nitriles after TCP. In addition, by introducing catalysts (KOH for pyrrole and Al2O3 for cyclo-Gly–Pro) to TCP, the production quantities of pyrrole and cyclo-Gly–Pro increased to 30.79 mg g−1 and 38.88 mg g−1, respectively. This approach can be used to convert the significant animal biomass waste generated annually from animal culls into valued organonitrogen chemicals while circumventing NH3-dependent and petrochemical-dependent synthesis routes.
Graphical Abstract
生产高价值的有机氮化合物，特别是氮杂环化合物，需要人工固氮，同时消耗化石资源。为了避免使用这些能源和资源密集型过程，我们开发了一种可持续的策略，通过在大气压下的热化学转化过程(TCP)将富氮动物生物质转化为n-杂环。由于牛皮肤含有丰富的含氮氨基酸(如甘氨酸、脯氨酸和l-羟基脯氨酸)，经TCP处理后得到的n-杂环的比例很高(87.51%)。氨基酸组成更多样化的动物生物量(如肌肉)在TCP后产生了更高浓度的胺/酰胺和腈。此外，通过在TCP中引入催化剂(KOH催化吡咯，Al2O3催化环- gly - pro)，吡咯和环- gly - pro的产生量分别提高到30.79 mg g - 1和38.88 mg g - 1。这种方法可用于将每年从动物扑杀中产生的大量动物生物质废物转化为有价值的有机氮化学品，同时绕过依赖nh3和依赖石化的合成路线。图形抽象
{"title":"A sustainable thermochemical conversion of animal biomass to N-heterocycles","authors":"Yang Tang, Xiao Xiao, Chaojun Zhang, Xiaoling Wang, Junling Guo, Xuepin Liao","doi":"10.1186/s42825-022-00109-z","DOIUrl":"10.1186/s42825-022-00109-z","url":null,"abstract":"<div><p>The production of high-valued organonitrogen chemicals, especially N-heterocycles, requires artificial N<sub>2</sub> fixation accompanied by the consumption of fossil resources. To avoid the use of these energy- and resource-intensive processes, we develop a sustainable strategy to convert nitrogen-rich animal biomass into N-heterocycles through a thermochemical conversion process (TCP) under atmospheric pressure. A high percentage of N-heterocycles (87.51%) were obtained after the TCP of bovine skin due to the abundance of nitrogen-containing amino acids (e.g., glycine, proline, and <span>l</span>-hydroxyproline). Animal biomass with more diverse amino acid composition (e.g., muscles) yielded higher concentrations of amines/amides and nitriles after TCP. In addition, by introducing catalysts (KOH for pyrrole and Al<sub>2</sub>O<sub>3</sub> for cyclo-Gly–Pro) to TCP, the production quantities of pyrrole and cyclo-Gly–Pro increased to 30.79 mg g<sup>−1</sup> and 38.88 mg g<sup>−1</sup>, respectively. This approach can be used to convert the significant animal biomass waste generated annually from animal culls into valued organonitrogen chemicals while circumventing NH<sub>3</sub>-dependent and petrochemical-dependent synthesis routes.</p><h3>Graphical Abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-022-00109-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4495007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-05DOI: 10.1186/s42825-022-00108-0
Yuanhang Xiao, Jiajing Zhou, Chunhua Wang, Jinwei Zhang, Vera D. Radnaeva, Wei Lin
Restrictions on heavy metals, especially chromium, have encouraged alternative tanning systems that can reduce environmental and human health risks from conventional chrome-based tanning. In this work, metal-free combination tanning was developed by using vegetable tannins and a triazine-based syntan containing active chlorine groups (SACC). Specifically, the relationship between leather performance (e.g., hydrothermal stability and organoleptic properties) and technical protocols (e.g., types and dose of tannins) was systematically established. The optimized protocol involving a unique procedure (i.e., 10% SACC pre-tanning, shaving, and 25% wattle tanning) endowed the leather with high shrinkage temperature (~ 92 °C) and met the Chinese standards for shoe upper leather (QB/T 1873-2010). Our method not only produces zero chrome-containing solid wastes, but also uses ~ 75% less tannin for leather manufacture. The excellent leather performance was ascribed to the synergistic effects, where SACC and wattle diffused into collagen fibrils and may bind to collagen via covalent, hydrogen and ionic bonding, locking the hierarchical structure of collagen from microfibrils to fiber bundles. Moreover, we summarized these findings and proposed a diffusion-binding-locking mechanism, providing new insights for current tanning theory. Together with the biodegradable spent tanning liquor, this approach will underpin the development of sustainable leather manufacture.
{"title":"Sustainable metal-free leather manufacture via synergistic effects of triazine derivative and vegetable tannins","authors":"Yuanhang Xiao, Jiajing Zhou, Chunhua Wang, Jinwei Zhang, Vera D. Radnaeva, Wei Lin","doi":"10.1186/s42825-022-00108-0","DOIUrl":"10.1186/s42825-022-00108-0","url":null,"abstract":"<div><p>Restrictions on heavy metals, especially chromium, have encouraged alternative tanning systems that can reduce environmental and human health risks from conventional chrome-based tanning. In this work, metal-free combination tanning was developed by using vegetable tannins and a triazine-based syntan containing active chlorine groups (SACC). Specifically, the relationship between leather performance (e.g., hydrothermal stability and organoleptic properties) and technical protocols (e.g., types and dose of tannins) was systematically established. The optimized protocol involving a unique procedure (i.e., 10% SACC pre-tanning, shaving, and 25% wattle tanning) endowed the leather with high shrinkage temperature (~ 92 °C) and met the Chinese standards for shoe upper leather (QB/T 1873-2010). Our method not only produces zero chrome-containing solid wastes, but also uses ~ 75% less tannin for leather manufacture. The excellent leather performance was ascribed to the synergistic effects, where SACC and wattle diffused into collagen fibrils and may bind to collagen via covalent, hydrogen and ionic bonding, locking the hierarchical structure of collagen from microfibrils to fiber bundles. Moreover, we summarized these findings and proposed a diffusion-binding-locking mechanism, providing new insights for current tanning theory. Together with the biodegradable spent tanning liquor, this approach will underpin the development of sustainable leather manufacture.</p><h3>Graphical Abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-022-00108-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4545150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-05DOI: 10.1186/s42825-022-00107-1
Xiaoguang Li, Keya Sen, Yuqin Zhang, Yongqiang Tian, Bi Shi
Wet-salted skin, as a special artificial high-salt environment, is rich in protein, fat, collagen and other nutrient substrates, and is a rich resource of halotolerant and halophilic microorganisms. However, knowledge gaps regarding the microbial community structure and inter taxa associations of wet-salted skin are large. In this study, the spatiotemporal dynamics and community structure of microorganisms present on wet-salted goatskins were investigated using 16S rRNA gene amplicon sequencing and culturable technique. Alpha diversity analysis based on Sobs, Chao, Ace and Shannon indices revealed that microbial diversity on the wet-salted goatskins exhibited a trend of ‘down → up → down → flat’ with time. During preservation, genera belonging to the bacteria domain such as Acinetobacter, Weissella and Streptococcus were slowly dying out, whereas those belonging to halophilic archaea such as Natrialba and Haloterrigena were gradually flourishing. Moreover, to resist high-salt stress, microorganisms on the wet-salted goatskin gradually migrated from the outside to the inside, eventually leading to the microbial diversity inside the skin being the same as or even higher than that on the skin surface. Venn diagram analysis revealed that the strains of some genera, including Psychrobacter, Salimicrobium, Salinicola, Ornithinibacillus, Halomonas, Bacillus and Chromohalobacter, were distributed throughout the interior and exterior of the wet-salted goatskin and existed during various periods. Accordingly, 45 protease-producing halophilic or halotolerant microorganisms were isolated and screened from the wet-salted goatskin using the gradient dilution plate method. Importantly, 16S rRNA genes of some bacteria exhibited less than 99.5% similarity to valid published species, indicating that they likely are novel species and have a good potential for application.
{"title":"Spatiotemporal dynamics of the microbial diversity on salt-preserved goatskins assessed by culturing and 16S rRNA gene amplicon sequencing","authors":"Xiaoguang Li, Keya Sen, Yuqin Zhang, Yongqiang Tian, Bi Shi","doi":"10.1186/s42825-022-00107-1","DOIUrl":"10.1186/s42825-022-00107-1","url":null,"abstract":"<div><p>Wet-salted skin, as a special artificial high-salt environment, is rich in protein, fat, collagen and other nutrient substrates, and is a rich resource of halotolerant and halophilic microorganisms. However, knowledge gaps regarding the microbial community structure and inter taxa associations of wet-salted skin are large. In this study, the spatiotemporal dynamics and community structure of microorganisms present on wet-salted goatskins were investigated using 16S rRNA gene amplicon sequencing and culturable technique. Alpha diversity analysis based on Sobs, Chao, Ace and Shannon indices revealed that microbial diversity on the wet-salted goatskins exhibited a trend of ‘down → up → down → flat’ with time. During preservation, genera belonging to the bacteria domain such as <i>Acinetobacter</i>, <i>Weissella</i> and <i>Streptococcus</i> were slowly dying out, whereas those belonging to halophilic archaea such as <i>Natrialba</i> and <i>Haloterrigena</i> were gradually flourishing. Moreover, to resist high-salt stress, microorganisms on the wet-salted goatskin gradually migrated from the outside to the inside, eventually leading to the microbial diversity inside the skin being the same as or even higher than that on the skin surface. Venn diagram analysis revealed that the strains of some genera, including <i>Psychrobacter</i>, <i>Salimicrobium</i>, <i>Salinicola</i>, <i>Ornithinibacillus</i>, <i>Halomonas</i>, <i>Bacillus</i> and <i>Chromohalobacter</i>, were distributed throughout the interior and exterior of the wet-salted goatskin and existed during various periods. Accordingly, 45 protease-producing halophilic or halotolerant microorganisms were isolated and screened from the wet-salted goatskin using the gradient dilution plate method. Importantly, 16S rRNA genes of some bacteria exhibited less than 99.5% similarity to valid published species, indicating that they likely are novel species and have a good potential for application.</p><h3>Graphical Abstract</h3>\u0000 <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\u0000 </div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-022-00107-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80621739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-02DOI: 10.1186/s42825-022-00106-2
Yuanyuan Li, Jianming Xiong, Yi Hu, Wenjun Miao, He Huang
Nowadays, multidrug-resistant (MDR) bacterial infectious diseases has become a thorny issue in the healthcare field. Owning to its intrinsic merits, photodynamic therapy (PDT) shows tremendous strengths in fighting against MDR bacterial infections. However, most photodynamic nanoplatforms exhibit unsatisfactory targeting efficiency towards bacteria and infection site, which may compromise the bactericidal effect of PDT. Herein, we firstly reported a bacteria-targeted collagen-based nanoparticle, named Ce6/Col/MM, for treating methicillin-resistant Staphylococcus aureus (MRSA)-infected wound. Ce6/Col/MM was fabricated by wrapping chlorin e6 (Ce6)-loaded collagen-based nanoparticles with macrophage membrane (MM), showing excellent photodynamic activity and good biocompatibility. In vitro studies demonstrated that Ce6/Col/MM could target to bacteria and then exhibit prominent antibacterial capacity against planktonic MRSA under light irradiation. Furthermore, the treatment of MRSA-infected wound in mice with Ce6/Col/MM plus light illumination resulted in potent bacterial inactivation and accelerated wound healing, accompanied by favorable histological compatibility. Collectively, Ce6/Col/MM with superior targeting ability towards bacteria, effective photodynamic antibacterial potency and minimal safety concerns, might be a powerful bactericidal nanoagent for treating infections caused by MDR bacteria.
{"title":"Wrapping collagen-based nanoparticle with macrophage membrane for treating multidrug-resistant bacterial infection","authors":"Yuanyuan Li, Jianming Xiong, Yi Hu, Wenjun Miao, He Huang","doi":"10.1186/s42825-022-00106-2","DOIUrl":"10.1186/s42825-022-00106-2","url":null,"abstract":"<div><p>Nowadays, multidrug-resistant (MDR) bacterial infectious diseases has become a thorny issue in the healthcare field. Owning to its intrinsic merits, photodynamic therapy (PDT) shows tremendous strengths in fighting against MDR bacterial infections. However, most photodynamic nanoplatforms exhibit unsatisfactory targeting efficiency towards bacteria and infection site, which may compromise the bactericidal effect of PDT. Herein, we firstly reported a bacteria-targeted collagen-based nanoparticle, named Ce6/Col/MM, for treating methicillin-resistant <i>Staphylococcus aureus</i> (MRSA)-infected wound. Ce6/Col/MM was fabricated by wrapping chlorin e6 (Ce6)-loaded collagen-based nanoparticles with macrophage membrane (MM), showing excellent photodynamic activity and good biocompatibility. In vitro studies demonstrated that Ce6/Col/MM could target to bacteria and then exhibit prominent antibacterial capacity against planktonic MRSA under light irradiation. Furthermore, the treatment of MRSA-infected wound in mice with Ce6/Col/MM plus light illumination resulted in potent bacterial inactivation and accelerated wound healing, accompanied by favorable histological compatibility. Collectively, Ce6/Col/MM with superior targeting ability towards bacteria, effective photodynamic antibacterial potency and minimal safety concerns, might be a powerful bactericidal nanoagent for treating infections caused by MDR bacteria.</p><h3>Graphical Abstract</h3>\u0000 <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\u0000 </div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-022-00106-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81221084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.1186/s42825-022-00103-5
Jing Wang, Hong Hu, Jian Wang, He Qiu, Yongli Gao, Yang Xu, Zhanhong Liu, Yajun Tang, Lu Song, John Ramshaw, Hai Lin, Xingdong Zhang
Collagen made a tremendous impact in the field of regenerative medicine as a bioactive material. For decades, collagen has been used not only as a scaffolding material but also as an active component in regulating cells' biological behavior and phenotype. However, animal-derived collagen as a major source suffered from problems of immunogenicity, risk of viral infection, and the unclear relationship between bioactive sequence and function. Recombinant humanized collagen (rhCol) provided alternatives for regenerative medicine with more controllable risks. However, the characterization of rhCol and the interaction between rhCol and cells still need further investigation, including cell behavior and phenotype. The current study preliminarily demonstrated that recombinant humanized collagen type III (rhCol III) conformed to the theoretical amino acid sequence and had an advanced structure resembling bovine collagen. Furthermore, rhCol III could facilitate basal biological behaviors of human skin fibroblasts, such as adhesion, proliferation and migration. rhCol III was beneficial for some extracellular matrix-expressing cell phenotypes. The study would shed light on the mechanism research of rhCol and cell interactions and further understanding of effectiveness in tissue regeneration.
{"title":"Characterization of recombinant humanized collagen type III and its influence on cell behavior and phenotype","authors":"Jing Wang, Hong Hu, Jian Wang, He Qiu, Yongli Gao, Yang Xu, Zhanhong Liu, Yajun Tang, Lu Song, John Ramshaw, Hai Lin, Xingdong Zhang","doi":"10.1186/s42825-022-00103-5","DOIUrl":"10.1186/s42825-022-00103-5","url":null,"abstract":"<div><p>Collagen made a tremendous impact in the field of regenerative medicine as a bioactive material. For decades, collagen has been used not only as a scaffolding material but also as an active component in regulating cells' biological behavior and phenotype. However, animal-derived collagen as a major source suffered from problems of immunogenicity, risk of viral infection, and the unclear relationship between bioactive sequence and function. Recombinant humanized collagen (rhCol) provided alternatives for regenerative medicine with more controllable risks. However, the characterization of rhCol and the interaction between rhCol and cells still need further investigation, including cell behavior and phenotype. The current study preliminarily demonstrated that recombinant humanized collagen type III (rhCol III) conformed to the theoretical amino acid sequence and had an advanced structure resembling bovine collagen. Furthermore, rhCol III could facilitate basal biological behaviors of human skin fibroblasts, such as adhesion, proliferation and migration. rhCol III was beneficial for some extracellular matrix-expressing cell phenotypes. The study would shed light on the mechanism research of rhCol and cell interactions and further understanding of effectiveness in tissue regeneration.\u0000</p><h3>Graphical abstract</h3>\u0000 <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\u0000 </div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-022-00103-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73312119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-07DOI: 10.1186/s42825-022-00102-6
Jiayi Zhu, Zhulian Li, Yaping Zou, Gonggong Lu, Alfredo Ronca, Ugo D’Amora, Jie Liang, Yujiang Fan, Xingdong Zhang, Yong Sun
In tissue engineering, bioactive materials play an important role, providing structural support, cell regulation and establishing a suitable microenvironment to promote tissue regeneration. As the main component of extracellular matrix, collagen is an important natural bioactive material and it has been widely used in scientific research and clinical applications. Collagen is available from a wide range of animal origin, it can be produced by synthesis or through recombinant protein production systems. The use of pure collagen has inherent disadvantages in terms of physico-chemical properties. For this reason, a processed collagen in different ways can better match the specific requirements as biomaterial for tissue repair. Here, collagen may be used in bone/cartilage regeneration, skin regeneration, cardiovascular repair and other fields, by following different processing methods, including cross-linked collagen, complex, structured collagen, mineralized collagen, carrier and other forms, promoting the development of tissue engineering. This review summarizes a wide range of applications of collagen-based biomaterials and their recent progress in several tissue regeneration fields. Furthermore, the application prospect of bioactive materials based on collagen was outlooked, aiming at inspiring more new progress and advancements in tissue engineering research.
{"title":"Advanced application of collagen-based biomaterials in tissue repair and restoration","authors":"Jiayi Zhu, Zhulian Li, Yaping Zou, Gonggong Lu, Alfredo Ronca, Ugo D’Amora, Jie Liang, Yujiang Fan, Xingdong Zhang, Yong Sun","doi":"10.1186/s42825-022-00102-6","DOIUrl":"10.1186/s42825-022-00102-6","url":null,"abstract":"<div><p>In tissue engineering, bioactive materials play an important role, providing structural support, cell regulation and establishing a suitable microenvironment to promote tissue regeneration. As the main component of extracellular matrix, collagen is an important natural bioactive material and it has been widely used in scientific research and clinical applications. Collagen is available from a wide range of animal origin, it can be produced by synthesis or through recombinant protein production systems. The use of pure collagen has inherent disadvantages in terms of physico-chemical properties. For this reason, a processed collagen in different ways can better match the specific requirements as biomaterial for tissue repair. Here, collagen may be used in bone/cartilage regeneration, skin regeneration, cardiovascular repair and other fields, by following different processing methods, including cross-linked collagen, complex, structured collagen, mineralized collagen, carrier and other forms, promoting the development of tissue engineering. This review summarizes a wide range of applications of collagen-based biomaterials and their recent progress in several tissue regeneration fields. Furthermore, the application prospect of bioactive materials based on collagen was outlooked, aiming at inspiring more new progress and advancements in tissue engineering research.</p><h3>Graphical Abstract</h3>\u0000 <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\u0000 </div>","PeriodicalId":640,"journal":{"name":"Journal of Leather Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://JLSE.SpringerOpen.com/counter/pdf/10.1186/s42825-022-00102-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81482422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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