Journal of Materials Science: Materials in Medicine最新文献_第10页

Cobalt-containing borate bioactive glass fibers for treatment of diabetic wound 治疗糖尿病伤口的含钴硼酸盐生物活性玻璃纤维

IF 3.7 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2023-08-02 DOI: 10.1007/s10856-023-06741-3

Minhui Zhang, Aihua Yao, Fanrong Ai, Jian Lin, Qingge Fu, Deping Wang

Impaired angiogenesis is one of the predominant reasons for non-healing diabetic wounds. Cobalt is well known for its capacity to induce angiogenesis by stabilizing hypoxia-inducible factor-1α (HIF-1α) and subsequently inducing the production of vascular endothelial growth factor (VEGF). In this study, Co-containing borate bioactive glasses and their derived fibers were fabricated by partially replacing CaO in 1393B3 borate glass with CoO. Fourier transform infrared (FTIR) spectroscopy and nuclear magnetic resonance (NMR) analyses were performed to characterize the effect of Co incorporation on the glass structure, and the results showed that the substitution promoted the transformation of [BO₃] into [BO₄] units, which endow the glass with higher chemical durability and lower reaction rate with the simulated body fluid (SBF), thereby achieving sustained and controlled Co²⁺ ion release. In vitro biological assays were performed to assess the angiogenic potential of the Co-containing borate glass fibers. It was found that the released Co²⁺ ion significantly enhanced the proliferation, migration and tube formation of the Human Umbilical Vein Endothelial Cells (HUVECs) by upregulating the expression of angiogenesis-related proteins such as HIF-1α and VEGF. Finally. In vivo results demonstrated that the Co-containing fibers accelerated full-thickness skin wound healing in streptozotocin (STZ)-induced diabetic rat model by promoting angiogenesis and re-epithelialization.

Graphical Abstract

血管生成障碍是糖尿病创面不愈合的主要原因之一。众所周知，钴通过稳定缺氧诱导因子-1α (HIF-1α)并随后诱导血管内皮生长因子(VEGF)的产生来诱导血管生成。在本研究中，用CoO部分取代1393B3硼酸玻璃中的CaO，制备了含钴硼酸生物活性玻璃及其衍生纤维。傅里叶变换红外光谱(FTIR)和核磁共振(NMR)分析表征了Co加入对玻璃结构的影响，结果表明Co的加入促进了[BO3]向[BO4]单元的转化，使玻璃具有更高的化学耐久性和较低的与模拟体液(SBF)的反应速率，从而实现了Co2+离子的持续和可控释放。体外生物实验评估含钴硼酸玻璃纤维的血管生成潜能。研究发现，释放的Co2+离子通过上调HIF-1α和VEGF等血管生成相关蛋白的表达，显著促进人脐静脉内皮细胞(HUVECs)的增殖、迁移和成管。最后。体内实验结果表明，含钴纤维通过促进血管生成和再上皮化，促进链脲佐菌素(STZ)诱导的糖尿病大鼠模型全层皮肤创面愈合。图形抽象

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引用次数: 0

Targeted drug-loaded PLGA-PCL microspheres for specific and localized treatment of triple negative breast cancer 靶向载药PLGA-PCL微球用于特异性和局部治疗三阴性乳腺癌

IF 3.7 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2023-08-02 DOI: 10.1007/s10856-023-06738-y

Chukwudalu C. Nwazojie, John D. Obayemi, Ali A. Salifu, Sandra M. Borbor-Sawyer, Vanessa O. Uzonwanne, Chinyerem E. Onyekanne, Udom M. Akpan, Killian C. Onwudiwe, Josephine C. Oparah, Olushola S. Odusanya, Winston O. Soboyejo

The paper presents the results of the experimental and analytical study of targeted drug-loaded polymer-based microspheres made from blend polymer of polylactic-co-glycolic acid and polycaprolactone (PLGA-PCL) for targeted and localized cancer drug delivery. In vitro sustained release with detailed thermodynamically driven drug release kinetics, over a period of three months using encapsulated targeted drugs (prodigiosin-EphA2 or paclitaxel-EphA2) and control drugs [Prodigiosin (PGS), and paclitaxel (PTX)] were studied. Results from in vitro study showed a sustained and localized drug release that is well-characterized by non-Fickian Korsmeyer–Peppas kinetics model over the range of temperatures of 37 °C (body temperature), 41 °C, and 44 °C (hyperthermic temperatures). The in vitro alamar blue, and flow cytometry assays in the presence of the different drug-loaded polymer formulations resulted to cell death and cytotoxicity that was evidence through cell inhibition and late apoptosis on triple negative breast cancer (TNBC) cells (MDA-MB 231). In vivo studies carried out on groups of 4-week-old athymic nude mice that were induced with subcutaneous TNBC, showed that the localized release of the EphA2-conjugated drugs was effective in complete elimination of residual tumor after local surgical resection. Finally, ex vivo histopathological analysis carried out on the euthanized mice revealed no cytotoxicity and absence of breast cancer metastases in the liver, kidney, and lungs 12 weeks after treatment. The implications of the results are then discussed for the development of encapsulated EphA2-conjugated drugs formulation in the specific targeting, localized, and sustain drug release for the elimination of local recurred TNBC tumors after surgical resection.

Graphical Abstract

本文介绍了由聚乳酸-羟基乙酸-聚己内酯共混聚合物(PLGA-PCL)制备的靶向载药聚合物微球用于靶向和局部肿瘤药物递送的实验和分析研究结果。在三个月的时间里，研究了包封的靶向药物(Prodigiosin - epha2或paclitaxel- epha2)和对照药物(Prodigiosin (PGS)和paclitaxel (PTX))的体外缓释动力学。体外研究结果显示，在37°C(体温)、41°C和44°C(高温)的温度范围内，非菲氏Korsmeyer-Peppas动力学模型很好地表征了药物的持续和局部释放。体外alamar blue和流式细胞术检测显示，不同的载药聚合物配方会导致细胞死亡和细胞毒性，这一点通过三阴性乳腺癌(TNBC)细胞的细胞抑制和晚期凋亡得到了证明(MDA-MB 231)。对皮下TNBC诱导的4周龄胸腺裸小鼠进行的体内研究表明，局部手术切除后，epha2偶联药物的局部释放可以有效地完全消除残留肿瘤。最后，对安乐死小鼠进行的离体组织病理学分析显示，治疗12周后，没有细胞毒性，肝脏、肾脏和肺部没有乳腺癌转移。然后讨论了研究结果对开发epha2缀合的囊化药物制剂的意义，该制剂具有特异性靶向、局部和持续释放药物的功能，以消除手术切除后局部复发的TNBC肿瘤。图形抽象

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引用次数: 0

Biocompatibility analysis and chemical characterization of Mn-doped hydroxyapatite 掺锰羟基磷灰石的生物相容性分析及化学性质

IF 3.7 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2023-07-29 DOI: 10.1007/s10856-023-06744-0

L. S. Villaseñor-Cerón, D. Mendoza-Anaya, S. López-Ortiz, R. Rosales-Ibañez, J. J. Rodríguez-Martínez, M. I. Reyes-Valderrama, V. Rodríguez-Lugo

The present work studies the effect of Mn doping on the crystalline structure of the Hap synthesized by the hydrothermal method at 200 °C for 24 h, from Ca(OH)₂ and (NH₄)₂HPO₄, incorporating MnCl₂ to 0.1, 0.5, 1.0, 1.5 and 2.0 %wt of Mn concentrations. Samples were characterized by the X-Ray Diffraction technique, which revealed the diffraction peaks that corresponded to the hexagonal and monoclinic phase of the Hap; it was observed that the average size of crystallite decreased from 23.67 to 22.69 nm as the concentration of Mn increased. TEM shows that in all samples, there are two distributions of particle sizes; one corresponds to nanorods with several tens of nanometers in length, and the other in which the diameter and length are very close. FTIR analysis revealed absorption bands corresponding to the PO₄⁻³ and OH⁻ groups characteristic of the Hap. It was possible to establish a substitution mechanism between the Mn and the ions of Ca⁺² of the Hap. From the Alamar blue test, a cell viability of 86.88% ± 5 corresponding to the sample of Hap at 1.5 %wt Mn was obtained, considered non-cytotoxic according to ISO 10993-5. It also evaluated and demonstrated the good osteoinductive properties of the materials, which were verified by histology and immunofluorescence expression of osteogenic markers. Adhesion, viability, biocompatibility and osteoinductive properties, make these materials candidates for future applications in bone tissue engineering with likely uses in regenerative medicine.

Graphical Abstract

以Ca(OH)2和(NH4)2HPO4为原料，加入浓度分别为0.1、0.5、1.0、1.5和2.0% wt的MnCl2，在200℃条件下，水热法制备了Mn掺杂对羟基磷灰石晶体结构的影响。用x射线衍射技术对样品进行了表征，发现衍射峰对应于Hap的六方相和单斜相;随着Mn浓度的增加，晶粒的平均尺寸由23.67 nm减小到22.69 nm。透射电镜显示，在所有样品中，粒径均存在两种分布;一种是长度为几十纳米的纳米棒，另一种是直径和长度非常接近的纳米棒。FTIR分析显示了羟基磷灰石的PO4 - 3和OH -基团特征。可以建立Mn与Hap的Ca+2离子之间的取代机制。从Alamar蓝试验中，获得的细胞活力为86.88%±5，对应于1.5% wt Mn的Hap样品，根据ISO 10993-5被认为是无细胞毒性的。通过组织学和成骨标志物的免疫荧光表达验证了材料良好的成骨诱导性能。粘附性、生存能力、生物相容性和骨诱导性能使这些材料成为未来骨组织工程的候选材料，并可能用于再生医学。图形抽象

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引用次数: 1

Composite material consisting of microporous beta-TCP ceramic and alginate-dialdehyde-gelatin for controlled dual release of clindamycin and bone morphogenetic protein 2 由微孔β - tcp陶瓷和海藻酸-二醛-明胶组成的复合材料，用于克林霉素和骨形态发生蛋白2的控制双释放

IF 3.7 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2023-07-27 DOI: 10.1007/s10856-023-06743-1

Lucas Ritschl, Pia Schilling, Annette Wittmer, Marc Bohner, Anke Bernstein, Hagen Schmal, Michael Seidenstuecker

The aim of this study was to produce a composite of microporous β-TCP filled with alginate-gelatin crosslinked hydrogel, clindamycin and bone morphogenetic protein (BMP-2) to prolong the drug-release behaviour for up to 28 days. The most promising alginate-di-aldehyde(ADA)-gelatin gel for drug release from microcapsules was used to fill microporous β-TCP ceramics under directional flow in a special loading chamber. Dual release of clindamycin and BMP-2 was measured on days 1, 2, 3, 6, 9, 14, 21 and 28 by high performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA). After release, the microbial efficacy of the clindamycin was checked and the biocompatibility of the composite was tested in cell culture. Clindamycin and the model substance FITC-protein A were released from microcapsules over 28 days. The clindamycin burst release was 43 ± 1%. For the loaded ceramics, a clindamycin release above the minimal inhibitory concentration (MIC) until day 9 and a burst release of 90.56 ± 2.96% were detected. BMP-2 was released from the loaded ceramics in low concentrations over 28 days. The release of active substances from β-TCP and hydrogel have already been extensively studied. Directional flow loading is a special procedure in which the ceramic could act as a stabilizer in the bone and, as a biodegradable system, enables a single-stage surgical procedure. Whether ADA-gelatin gel is suitable for this procedure as a more biodegradable alternative to pure alginate or whether a dual release is possible in this composite has not yet been investigated.

Graphical Abstract

本研究的目的是制备一种微孔β-TCP复合材料，填充海藻酸-明胶交联水凝胶、克林霉素和骨形态发生蛋白(BMP-2)，以延长药物释放行为长达28天。采用海藻酸二醛明胶(ADA)作为微胶囊释药凝胶，在定向流动条件下填充微孔β-TCP陶瓷。采用高效液相色谱法(HPLC)和酶联免疫吸附法(ELISA)检测克林霉素和BMP-2在第1、2、3、6、9、14、21和28天的双释放度。释放后检测克林霉素的微生物功效，并在细胞培养中检测复合材料的生物相容性。28 d后克林霉素和模型物质FITC-protein A从微胶囊中释放。克林霉素爆发释放量为43±1%。对于负载陶瓷，直到第9天，克林霉素的释放量都高于最低抑制浓度(MIC)，释放量为90.56±2.96%。在28天内，BMP-2以低浓度从加载的陶瓷中释放出来。β-TCP和水凝胶中活性物质的释放已被广泛研究。定向流加载是一种特殊的手术，其中陶瓷可以作为骨骼的稳定剂，作为一种可生物降解的系统，可以实现单阶段手术。ada -明胶是否适合作为一种更可生物降解的纯海藻酸盐替代品，或者这种复合材料是否可能双重释放，目前还没有研究。图形抽象

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引用次数: 0

Beta-TCP scaffolds with rationally designed macro-micro hierarchical structure improved angio/osteo-genesis capability for bone regeneration 具有合理设计的宏观-微观分层结构的 Beta-TCP 支架提高了骨再生的血管/骨生成能力

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2023-07-24 DOI: 10.1007/s10856-023-06733-3

Jianlang Feng, Junjie Liu, Yingqu Wang, Jingjing Diao, Yudi Kuang, Naru Zhao

The design of hierarchical porous structure in scaffolds is crucial for bone defect regenerative repair. However, bioceramic materials present a challenge in precisely constructing designed micropores owing to the limitation of forming process. To investigate micropore shape influences bone regeneration in bioceramic scaffolds with macropores, hierarchical porous scaffolds with interconnective macropores (~400 μm) and two types of micropores (spherical and fibrous) were prepared using a combination of direct ink writing (DIW) and template sacrifice methods. Compared to the scaffold with spherical micropores, the scaffold with highly interconnected fibrous micropores significantly improved cell adhesion and upregulated osteogenic and angiogenetic-related gene expression in mBMSCs and HUVECs, respectively. Furthermore, in vivo implantation experiments showed that hierarchical scaffolds with fibrous micropores accelerated the bone repair process significantly. This result can be attributed to the high interconnectivity of fibrous micropores, which promotes the transportation of nutrients and waste during bone regeneration. Our work demonstrates that hierarchical porous scaffold design, especially one with a fibrous micropore structure, is a promising strategy for improving the bone regeneration performance of bioceramic scaffolds.

Graphical Abstract

支架中分层多孔结构的设计对于骨缺损再生修复至关重要。然而，由于成型工艺的限制，生物陶瓷材料在精确构建设计微孔方面面临挑战。为了研究微孔形状对具有大孔的生物陶瓷支架中骨质再生的影响，研究人员采用直接墨水写入（DIW）和模板牺牲相结合的方法制备了具有相互连接的大孔（约 400 μm）和两种微孔（球形和纤维状）的分层多孔支架。与球形微孔支架相比，具有高度相互连接的纤维状微孔的支架能显著提高细胞粘附性，并分别上调 mBMSCs 和 HUVECs 的成骨和血管生成相关基因的表达。此外，体内植入实验表明，带有纤维状微孔的分层支架能明显加速骨修复过程。这一结果可归因于纤维微孔的高度互联性，它促进了骨再生过程中营养物质和废物的运输。我们的工作表明，分层多孔支架设计，尤其是具有纤维状微孔结构的支架设计，是提高生物陶瓷支架骨再生性能的一种有前途的策略。

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引用次数: 0

Host–device interactions: exposure of lung epithelial cells and fibroblasts to nickel, titanium, or nitinol affect proliferation, reactive oxygen species production, and cellular signaling 宿主与设备之间的相互作用：肺上皮细胞和成纤维细胞接触镍、钛或镍钛诺后会影响增殖、活性氧生成和细胞信号传导

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2023-07-24 DOI: 10.1007/s10856-023-06742-2

Simon D. Pouwels, Alina Sigaeva, Shanna de Boer, Ilse A. Eichhorn, Lisanne Koll, Jeroen Kuipers, Romana Schirhagl, Irene H. Heijink, Janette K. Burgess, Dirk-Jan Slebos

Endoscopic implantation of medical devices for the treatment of lung diseases, including airway stents, unidirectional valves and coils, is readily used to treat central airway disease and emphysema. However, granulation and fibrotic tissue formation impairs treatment effectiveness. To date little is known about the interaction between implanted devices, often made from metals, such as nickel, titanium or nitinol, and cells in the airways. Here, we study the response of lung epithelial cells and fibroblasts to implant device materials. The adhesion and proliferation of bronchial epithelial cells and lung fibroblasts upon exposure to 10 × 3 × 1 mm pieces of nickel, titanium or nitinol is examined using light and scanning electron microscopy. Pro-inflammatory cytokine mRNA expression and release, signaling kinase activity and intracellular free radical production are assessed. Nitinol, and to a lesser extent nickel and titanium, surfaces support the attachment and growth of lung epithelial cells. Nitinol induces a rapid and significant alteration of kinase activity. Cells directly exposed to nickel or titanium produce free radicals, but those exposed to nitinol do not. The response of lung epithelial cells and fibroblasts depends on the metal type to which they are exposed. Nitinol induces cellular surface growth and the induction of kinase activity, while exposure of lung epithelial cells to nickel and titanium induces free radical production, but nitinol does not.

Graphical Abstract

内窥镜植入治疗肺部疾病的医疗器械，包括气道支架、单向活瓣和线圈，可用于治疗中央气道疾病和肺气肿。然而，肉芽和纤维组织的形成会影响治疗效果。迄今为止，人们对植入装置（通常由镍、钛或镍钛诺等金属制成）与气道细胞之间的相互作用知之甚少。在这里，我们研究了肺上皮细胞和成纤维细胞对植入装置材料的反应。我们使用光学显微镜和扫描电子显微镜研究了支气管上皮细胞和肺成纤维细胞在接触 10 × 3 × 1 毫米的镍、钛或镍钛诺材料后的粘附和增殖情况。评估了促炎细胞因子 mRNA 的表达和释放、信号激酶活性和细胞内自由基的产生。镍钛诺表面支持肺上皮细胞的附着和生长，其次是镍和钛。镍钛诺诱导激酶活性发生快速而显著的变化。直接接触镍或钛的细胞会产生自由基，而接触镍钛诺的细胞则不会。肺上皮细胞和成纤维细胞的反应取决于它们所接触的金属类型。镍钛诺诱导细胞表面生长和诱导激酶活性，而肺上皮细胞暴露于镍和钛会诱导自由基的产生，但镍钛诺不会。

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引用次数: 0

A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro 三层脱细胞脱水人羊膜支架支持体外人腱鞘细胞的细胞功能

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2023-07-24 DOI: 10.1007/s10856-023-06740-4

Yong Mao, Nikita John, Nicole M. Protzman, Desiree Long, Raja Sivalenka, Shamshad Azimi, Brandon Mirabile, Robert Pouliot, Anna Gosiewska, Robert J. Hariri, Stephen A. Brigido

Differences in scaffold design have the potential to influence cell-scaffold interactions. This study sought to determine whether a tri-layer design influences the cellular function of human tenocytes in vitro. The single-layer decellularized, dehydrated human amniotic membrane (DDHAM) and the tri-layer DDHAM (DDHAM-3L) similarly supported tenocyte function as evidenced by improved cell growth and migration, reduced dedifferentiation, and an attenuated inflammatory response. The tri-layer design provides a mechanically more robust scaffold without altering biological activity.

Graphical Abstract

支架设计的不同有可能影响细胞与支架之间的相互作用。本研究试图确定三层设计是否会影响体外人类腱细胞的细胞功能。单层脱细胞脱水人羊膜（DDHAM）和三层脱细胞脱水人羊膜（DDHAM-3L）同样支持腱细胞功能，这表现在细胞生长和迁移得到改善、去分化减少以及炎症反应减弱。三层设计在不改变生物活性的情况下提供了一种机械上更坚固的支架。

引用次数: 0

A grooved conduit combined with decellularized tissues for peripheral nerve regeneration 结合脱细胞组织的沟槽导管用于外周神经再生

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2023-07-21 DOI: 10.1007/s10856-023-06737-z

Enxing Yu, Zhiwu Chen, Yuye Huang, Yibing Wu, Zonghuan Wang, Fangfang Wang, Miaoben Wu, Kailei Xu, Wei Peng

Peripheral nerve injury (PNI) is a common and severe clinical disease worldwide, which leads to a poor prognosis because of the complicated treatments and high morbidity. Autologous nerve grafting as the gold standard still cannot meet the needs of clinical nerve transplantation because of its low availability and limited size. The development of artificial nerve conduits was led to a novel direction for PNI treatment, while most of the currently developed artificial nerve conduits was lack biochemical cues to promote nerve regeneration. In this study, we designed a novel composite neural conduit by inserting decellularized the rat sciatic nerve or kidney in a poly (lactic-co-glycolic acid) (PLGA) grooved conduit. The nerve regeneration effect of all samples was analyzed using rat sciatic nerve defect model, where decellularized tissues and grooved PLGA conduit alone were used as controls. The degree of nerve regeneration was evaluated using the motor function, gastrocnemius recovery, and morphological and histological assessments suggested that the combination of a grooved conduit with decellularized tissues significantly promoted nerve regeneration compared with decellularized tissues and PLGA conduit alone. It is worth to note that the grooved conduits containing decellularized nerves have a promotive effect similar to that of autologous nerve grafting, suggesting that it could be an artificial nerve conduit used for clinical practice in the future.

Graphical Abstract

周围神经损伤（PNI）是世界范围内常见的严重临床疾病，因治疗复杂、发病率高而导致预后不良。自体神经移植作为金标准，因其可用性低、体积有限，仍不能满足临床神经移植的需要。人工神经导管的开发为PNI的治疗带来了新的方向，而目前开发的人工神经导管大多缺乏促进神经再生的生化线索。在本研究中，我们设计了一种新型复合神经导管，将脱细胞的大鼠坐骨神经或肾脏插入聚乳酸-共聚乙醇酸（PLGA）沟槽导管中。使用大鼠坐骨神经缺损模型分析了所有样本的神经再生效果，其中脱细胞组织和单独的开槽聚乳酸（PLGA）导管用作对照。通过运动功能、腓肠肌恢复、形态学和组织学评估来评价神经再生的程度，结果表明，与脱细胞组织和单独的 PLGA 导管相比，沟槽导管与脱细胞组织的组合能显著促进神经再生。值得注意的是，含有脱细胞神经的沟槽导管具有类似于自体神经移植的促进作用，这表明它可以作为一种人工神经导管用于未来的临床实践。

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引用次数: 0

A retrospective, comparative, clinical study of occlusion rate of peripherally inserted central catheters fabricated of poly(vinyl alcohol)-based hydrogel composite 关于聚乙烯醇基水凝胶复合材料外周置入中心导管闭塞率的回顾性比较临床研究

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2023-07-21 DOI: 10.1007/s10856-023-06736-0

Joseph Bunch, Brian Hanley, Daniel Donahue

Thrombotic accumulation is associated with surface interactions between blood proteins and vascular access devices. Catheter occlusion results from this process, and is a costly, common, occurrence with peripherally inserted central catheters (PICCs). Hydrophilic catheter materials exhibit antithrombotic properties. This retrospective study evaluates the occurrence of catheter occlusion of PICCs constructed of a poly(vinyl alcohol)-based hydrogel composite known as hydrophilic biomaterial (HBM), compared to thermoplastic polyurethane (TPU) control devices. A total of 121 PICCs, 60 HBM and 61 TPU, were placed in patients with a clinical need and were reviewed for the occurrence of catheter occlusion. The records review found that occlusions occurred in 0/60 (0.0%) of the HBM PICCs and 13/61 (21.3%) of TPU PICCs (p = 0.001). HBM exhibits favorable qualities for vascular access, most importantly its extreme hydrophilicity. Clinically, this may be responsible for the reduction in PICC occlusions, which could improve patient outcomes.

Graphical Abstract

血栓积聚与血液蛋白和血管通路设备之间的表面相互作用有关。导管闭塞就是这一过程的结果，外周置入中心导管（PICC）经常发生闭塞，代价高昂。亲水性导管材料具有抗血栓形成的特性。这项回顾性研究评估了由被称为亲水性生物材料（HBM）的聚乙烯醇基水凝胶复合材料制成的 PICC 与热塑性聚氨酯（TPU）控制装置相比发生导管闭塞的情况。共有 121 个 PICC（60 个 HBM 和 61 个 TPU）被植入有临床需求的患者体内，并对导管闭塞的发生情况进行了审查。记录审查发现，0/60（0.0%）的 HBM PICC 和 13/61（21.3%）的 TPU PICC 发生了闭塞（p = 0.001）。HBM 具有良好的血管通路特性，最重要的是它具有极强的亲水性。在临床上，这可能是减少 PICC 闭塞的原因，从而改善患者的治疗效果。

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引用次数: 0

Cissus quadrangularis L extract-loaded tricalcium phosphate reinforced natural polymer composite for guided bone regeneration 载磷酸三钙增强天然聚合物复合材料引导骨再生的研究

IF 3.7 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2023-07-19 DOI: 10.1007/s10856-023-06739-x

Lele Liao, Weihong Zhu, Cheng Tao, Ding Li, Minzhi Mao

Natural medicines plants are significant considerable attention as potential therapeutic agents for bone tissue engineering. Cissus quadrangularis L (CQ). is a potent therapeutic plant known for its own osteogenic properties. In this research work, a phytoconstituents-filled composite was produced by incorporating CQ extract with gelatin (Gel) and pectin (Pec) polymers collective through β- tricalcium phosphate (β-TCP) bioceramic via a green template method. The effect of CQ-filled composite morphology and chemical structural properties, in vitro cytotoxicity, cell proliferation, and differentiation was investigated. FTIR spectroscopic results indicated the prepared materials’ structural confirmation. The CQ extract was the alcoholic -OH merge with the hydroxyl and -NH groups in the range of 3000 cm⁻¹ to 3500 cm⁻¹. Scanning electron microscopy images showed that the β-TCP ceramic was perfectly embedded in Gel-Pec polymeric matrix, which is important for bone regeneration. In vitro cell culture results indicated that β-TCP/Gel-Pec/CQ composite provided 92.0% of a favorable substrate for mesenchymal stem cell viability. The gene expression and RT-PCR studies represent the materials with good osteogenic expression, especially the β-TCP/Gel-Pec/CQ composite is observed at 168.0% and 188.0% for RUNx2 and OCN, respectively. The result of the physicochemical characterizations and cell viability studies suggest that CQ-loaded β-TCP/Gel-Pec composite can serve as a potential biomaterial for bone tissue repair and regeneration.

Graphical Abstract

天然药物植物作为潜在的骨组织工程治疗剂受到广泛关注。四棱草(CQ)。是一种有效的治疗植物，以其自身的成骨特性而闻名。本研究采用绿色模板法，将CQ提取物与明胶(Gel)和果胶(Pec)聚合物通过β-磷酸三钙(β- tcp)生物陶瓷聚合，制备了一种植物成分填充复合材料。研究了cq填充复合材料的形态和化学结构特性、体外细胞毒性、细胞增殖和分化的影响。FTIR光谱结果证实了所制备材料的结构。CQ萃取物为乙醇-OH与羟基和-NH基团在3000 cm−1 ~ 3500 cm−1范围内合并。扫描电镜结果显示，β-TCP陶瓷在凝胶- pec聚合物基质中嵌入良好，对骨再生具有重要意义。体外细胞培养结果表明，β-TCP/Gel-Pec/CQ复合物为间充质干细胞提供了92.0%的有利底物。基因表达和RT-PCR研究表明，材料具有良好的成骨表达，特别是β-TCP/Gel-Pec/CQ复合物在RUNx2和OCN中分别达到168.0%和188.0%。理化表征和细胞活力研究结果表明，cq负载β-TCP/Gel-Pec复合材料可作为骨组织修复和再生的潜在生物材料。图形抽象

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引用次数: 1