Pub Date : 2025-06-17DOI: 10.1007/s10856-025-06903-5
Carolina Duque-Uribe, Valentina López Vargas, Ana Isabel Moreno Florez, Alejandro Pelaez-Vargas, Alex Ossa, Carolina Cárdenas-Ramírez, Sebastián Restrepo-Vélez, Andrés Felipe Vásquez, Claudia Garcia
Ceramic stereolithography scaffolds with designs based on triple periodic minimal surfaces (TPMS) were developed for potential applications in bone tissue regeneration. An acrylic-based resin with calcium phosphate nanoparticles were used. Particles were synthesized via Combustion in solution, resulting in hydroxyapatite and β-TCP phases. Suspensions with 35, 40, and 50 vol% particles, using a 10 wt% of dispersant, were prepared and rheologically characterized to ensure suitable viscosities for printing, and were used to print gyroid scaffolds by DLP technique. The suspension with the highest ceramic load demonstrated the highest viscosity. The green bodies were morphologically and mechanically characterized before and after sintering. Volumetric shrinkage, morphological characteristics by digital and FE-SEM images, and compressive strength were evaluated. Polymeric-ceramic (Hybrid) scaffolds before sintering exhibited better compressive strength than sintered ones. Ceramic scaffolds achieved compressive strength values up to 0.9 MPa, comparable to those of cancellous and cortical bone. The optimal scaffolds (50CPF) were subjected to degradation tests in PBS and were impregnated with ethanolic extract of propolis from Arauca, Colombia, for biological analysis using the L929 cell line. The results indicate that ceramic stereolithography is an effective technique to produce scaffolds with optimal characteristics for potential applications in bone tissue regeneration.
{"title":"Obtaining biocompatible ceramic scaffolds of calcium phosphates through ceramic stereolithography","authors":"Carolina Duque-Uribe, Valentina López Vargas, Ana Isabel Moreno Florez, Alejandro Pelaez-Vargas, Alex Ossa, Carolina Cárdenas-Ramírez, Sebastián Restrepo-Vélez, Andrés Felipe Vásquez, Claudia Garcia","doi":"10.1007/s10856-025-06903-5","DOIUrl":"10.1007/s10856-025-06903-5","url":null,"abstract":"<div><p>Ceramic stereolithography scaffolds with designs based on triple periodic minimal surfaces (TPMS) were developed for potential applications in bone tissue regeneration. An acrylic-based resin with calcium phosphate nanoparticles were used. Particles were synthesized via Combustion in solution, resulting in hydroxyapatite and β-TCP phases. Suspensions with 35, 40, and 50 vol% particles, using a 10 wt% of dispersant, were prepared and rheologically characterized to ensure suitable viscosities for printing, and were used to print gyroid scaffolds by DLP technique. The suspension with the highest ceramic load demonstrated the highest viscosity. The green bodies were morphologically and mechanically characterized before and after sintering. Volumetric shrinkage, morphological characteristics by digital and FE-SEM images, and compressive strength were evaluated. Polymeric-ceramic (Hybrid) scaffolds before sintering exhibited better compressive strength than sintered ones. Ceramic scaffolds achieved compressive strength values up to 0.9 MPa, comparable to those of cancellous and cortical bone. The optimal scaffolds (50CPF) were subjected to degradation tests in PBS and were impregnated with ethanolic extract of propolis from Arauca, Colombia, for biological analysis using the L929 cell line. The results indicate that ceramic stereolithography is an effective technique to produce scaffolds with optimal characteristics for potential applications in bone tissue regeneration.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12174250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Owing to its extremely high prevalence and the distressing consequence of tooth loss, periodontitis has attracted substantial research attention. In light of these conditions, graphene-based biomaterials have emerged as a potentially promising approach for periodontal regeneration. This study focuses on the synthesis of polydopamine-coated reduced graphene oxide (RGO@PDA), designed to harness the anti-inflammatory properties of dopamine and the osteogenic potential of graphene oxide for synergistic periodontitis treatment. RGO@PDA was synthesized through a 12-h magnetic stirring process of graphene oxide and dopamine at room temperature. This water-dispersible and biocompatible compound demonstrated remarkable efficacy in enhancing osteogenic differentiation in rat bone mesenchymal stem cells (rBMSCs), evidenced by increased alkaline phosphatase activity, mineralization, and the upregulation of osteogenic genes and proteins. Furthermore, RGO@PDA showed significant capabilities in scavenging reactive oxygen species (ROS) and reducing proinflammatory factor expression. In vivo experiments revealed that RGO@PDA not only alleviated periodontal inflammation but also promoted alveolar bone repair in periodontitis-afflicted rats. These findings underscore RGO@PDA’s dual anti-inflammatory and osteogenic effects, highlighting its potential as a transformative treatment for periodontitis.
{"title":"Synergistic effects of polydopamine-coated reduced graphene oxide on osteogenesis and anti-inflammation in periodontitis","authors":"Xiaoge Jiang, Xinyi Chen, Qiming Li, Xinyi Li, Kaiwen Zhang, Jiazhen Jiang, Xinrui Men, Wei-Cho Chiou, Song Chen","doi":"10.1007/s10856-025-06905-3","DOIUrl":"10.1007/s10856-025-06905-3","url":null,"abstract":"<div><p>Owing to its extremely high prevalence and the distressing consequence of tooth loss, periodontitis has attracted substantial research attention. In light of these conditions, graphene-based biomaterials have emerged as a potentially promising approach for periodontal regeneration. This study focuses on the synthesis of polydopamine-coated reduced graphene oxide (RGO@PDA), designed to harness the anti-inflammatory properties of dopamine and the osteogenic potential of graphene oxide for synergistic periodontitis treatment. RGO@PDA was synthesized through a 12-h magnetic stirring process of graphene oxide and dopamine at room temperature. This water-dispersible and biocompatible compound demonstrated remarkable efficacy in enhancing osteogenic differentiation in rat bone mesenchymal stem cells (rBMSCs), evidenced by increased alkaline phosphatase activity, mineralization, and the upregulation of osteogenic genes and proteins. Furthermore, RGO@PDA showed significant capabilities in scavenging reactive oxygen species (ROS) and reducing proinflammatory factor expression. In vivo experiments revealed that RGO@PDA not only alleviated periodontal inflammation but also promoted alveolar bone repair in periodontitis-afflicted rats. These findings underscore RGO@PDA’s dual anti-inflammatory and osteogenic effects, highlighting its potential as a transformative treatment for periodontitis.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12165884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The rapid advancement of three-dimensional (3D) printing in dentistry has prompted comparisons between the mechanical properties of polylactic acid (PLA) samples fabricated using material extrusion (MEx) and resin samples produced using digital light processing (DLP). This study aims to assess the potential of replacing resin-based models with PLA models in clinical settings by evaluating the mechanical properties and accuracy of MEx-printed PLA and DLP-printed resin samples. The investigated materials include pure PLA, a PLA composite containing gypsum, and a clinically approved resin material. Strength and hardness tests were conducted using custom-made samples measuring 16 × 4 × 2 mm3. Additionally, oral cavity scans were used to generate oral models for each material to assess their accuracy, trueness, and precision. The results indicated that pure PLA exhibited the highest flexural modulus (2055 ± 217.70 MPa) and compression modulus (2.40 ± 0.14 GPa). The PLA-Gypsum composite displayed the highest hardness (19.48 ± 2.12 HV1). As for the trueness of the oral models, there were no statistically significant differences between the models made from the three materials. However, the PLA-Gypsum composite demonstrated the best precision (23.84 ± 4.12 μm). These findings suggest that both PLA materials have significant potential to replace DLP-produced resin models in the clinical applications.
{"title":"The potential of PLA based dental models by material extrusion 3D printing: an in vitro study investigating mechanical properties and dimensional accuracy","authors":"Jiandong Li, Yuyang Mao, Jamila Yassine, Nico Henning, Alexey Unkovskiy, Florian Beuer, Franziska Schmidt","doi":"10.1007/s10856-025-06899-y","DOIUrl":"10.1007/s10856-025-06899-y","url":null,"abstract":"<div><p>The rapid advancement of three-dimensional (3D) printing in dentistry has prompted comparisons between the mechanical properties of polylactic acid (PLA) samples fabricated using material extrusion (MEx) and resin samples produced using digital light processing (DLP). This study aims to assess the potential of replacing resin-based models with PLA models in clinical settings by evaluating the mechanical properties and accuracy of MEx-printed PLA and DLP-printed resin samples. The investigated materials include pure PLA, a PLA composite containing gypsum, and a clinically approved resin material. Strength and hardness tests were conducted using custom-made samples measuring 16 × 4 × 2 mm<sup>3</sup>. Additionally, oral cavity scans were used to generate oral models for each material to assess their accuracy, trueness, and precision. The results indicated that pure PLA exhibited the highest flexural modulus (2055 ± 217.70 MPa) and compression modulus (2.40 ± 0.14 GPa). The PLA-Gypsum composite displayed the highest hardness (19.48 ± 2.12 HV1). As for the trueness of the oral models, there were no statistically significant differences between the models made from the three materials. However, the PLA-Gypsum composite demonstrated the best precision (23.84 ± 4.12 μm). These findings suggest that both PLA materials have significant potential to replace DLP-produced resin models in the clinical applications.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-09DOI: 10.1007/s10856-024-06845-4
Lays Fernanda Nunes Dourado, Fernanda Aparecida Silva Vieira, Thomas Toshio Inoue, Silvia Ligorio Fialho, Lutiana Amaral de Melo, Vinicius Viana Pereira, Juliana Rios de Simoni, Matheus Soares Siman, Paulo Ferrara de Almeida Cunha, Armando da Silva Cunha Júnior
Changes in collagen orientation and distribution on the corneas lead to the development of diseases characterized by progressive thinning, such as keratoconus. Part of people diagnosed with keratoconus require a corneal graft, which has availability as a major limiting factor. In this scenario, new approaches have been tested to obtain substitute tissues. Porcine cornea has been receiving increasing attention due to its ease of obtaining, biomechanical properties similar to those of human tissue and lower antigenicity. Based on this, the objective of this study was to evaluate the biocompatibility of porcine stroma decellularized by sodium dodecyl sulfate (SDS) through interlamellar implantation in rabbit corneas. The obtained results showed that the lenticule intrastromal implantation was successfully performed and did not elicit rejection. Furthermore, the implanted stroma was able to promote an increase in the thickness of the host cornea. Microscopic analyses revealed that the tissue was well-adhered and the collagen fibrils were more aligned on its periphery. Therefore, it is concluded that the implantation of decellularized porcine stroma occurred satisfactorily and represents a promising alternative to replace human tissue.
{"title":"Interlamellar keratoplasty for implantation of decellularized porcine corneal lenticule in a rabbit for corneal thickening","authors":"Lays Fernanda Nunes Dourado, Fernanda Aparecida Silva Vieira, Thomas Toshio Inoue, Silvia Ligorio Fialho, Lutiana Amaral de Melo, Vinicius Viana Pereira, Juliana Rios de Simoni, Matheus Soares Siman, Paulo Ferrara de Almeida Cunha, Armando da Silva Cunha Júnior","doi":"10.1007/s10856-024-06845-4","DOIUrl":"10.1007/s10856-024-06845-4","url":null,"abstract":"<div><p>Changes in collagen orientation and distribution on the corneas lead to the development of diseases characterized by progressive thinning, such as keratoconus. Part of people diagnosed with keratoconus require a corneal graft, which has availability as a major limiting factor. In this scenario, new approaches have been tested to obtain substitute tissues. Porcine cornea has been receiving increasing attention due to its ease of obtaining, biomechanical properties similar to those of human tissue and lower antigenicity. Based on this, the objective of this study was to evaluate the biocompatibility of porcine stroma decellularized by sodium dodecyl sulfate (SDS) through interlamellar implantation in rabbit corneas. The obtained results showed that the lenticule intrastromal implantation was successfully performed and did not elicit rejection. Furthermore, the implanted stroma was able to promote an increase in the thickness of the host cornea. Microscopic analyses revealed that the tissue was well-adhered and the collagen fibrils were more aligned on its periphery. Therefore, it is concluded that the implantation of decellularized porcine stroma occurred satisfactorily and represents a promising alternative to replace human tissue.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12148985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144245581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-06DOI: 10.1007/s10856-025-06900-8
Menghui Chi, Leyuan Zhang, Bing Deng, Jing Lin, Qian Wang, Lina Zhang, Min Yin, Lingwen Gu, Daohao Li, Guiqiu Zhao, Cui Li
The blindness rate of fungal keratitis is high, however, traditional therapy, like eye drops, has poor bioavailability. To make the traditional treatment more effective, new drug-loading system was explored, which can attach to the ocular surface to prolong the release time of natamycin (NATA). Sodium alginate (SA) has attractive properties of biocompatibility, biodegradability, which have been exploited to be natural-origin polymer of drug release. Because of special egg box structure of SA, the sodium ions in the structure can be exchanged with divalent cations through crosslinking, which could control the pore size inside the material and release rate of the loaded drug. Here, we utilized the composite of sodium alginate and polyethylene oxide (PEO) with natamycin loaded through crosslinking with calcium ion ethanol aqueous solution to delay drug release and treat fungal keratitis. The results from experiments proved that the membrane with the slowest rate of drug release was the group with the ratio of ethanol to water 2:1, and the 1% natamycin-loaded films could effectively inhibit the growth of Aspergillus fumigatus. SA-PEO membranes could reduce the inflammatory response. Conclusively, NATA-SA-PEO films could be considered a useful approach to prolong ocular natamycin maintenance and improve the outcome of fungal keratitis.
{"title":"Moderation of cross linkage of sodium alginate-polyethylene oxide films loaded with natamycin for treatment of Aspergillus fumigatus keratitis","authors":"Menghui Chi, Leyuan Zhang, Bing Deng, Jing Lin, Qian Wang, Lina Zhang, Min Yin, Lingwen Gu, Daohao Li, Guiqiu Zhao, Cui Li","doi":"10.1007/s10856-025-06900-8","DOIUrl":"10.1007/s10856-025-06900-8","url":null,"abstract":"<div><p>The blindness rate of fungal keratitis is high, however, traditional therapy, like eye drops, has poor bioavailability. To make the traditional treatment more effective, new drug-loading system was explored, which can attach to the ocular surface to prolong the release time of natamycin (NATA). Sodium alginate (SA) has attractive properties of biocompatibility, biodegradability, which have been exploited to be natural-origin polymer of drug release. Because of special egg box structure of SA, the sodium ions in the structure can be exchanged with divalent cations through crosslinking, which could control the pore size inside the material and release rate of the loaded drug. Here, we utilized the composite of sodium alginate and polyethylene oxide (PEO) with natamycin loaded through crosslinking with calcium ion ethanol aqueous solution to delay drug release and treat fungal keratitis. The results from experiments proved that the membrane with the slowest rate of drug release was the group with the ratio of ethanol to water 2:1, and the 1% natamycin-loaded films could effectively inhibit the growth of <i>Aspergillus fumigatus</i>. SA-PEO membranes could reduce the inflammatory response. Conclusively, NATA-SA-PEO films could be considered a useful approach to prolong ocular natamycin maintenance and improve the outcome of fungal keratitis.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-06DOI: 10.1007/s10856-024-06837-4
Zhao Wang, Jingjing Sun, Lin Jia, Ruilong Sheng
Glycopolymer drug delivery nanosystems have attracted increasing attention in the field of sustainable biomaterials and clinical biomedicine, while few studies addressed their intracellular drug delivery properties, endocytosis pathways, intracellular trafficking, autophagy behaviors and the effect of autophagy inhibitors. Based on our previous study, in this work, a pH-responsive glycopolymer (PMAgala18-b-P(MAA24-co-MAChol6)) was synthesized and used as a drug delivery carrier, to encapsulate antitumor drug doxorubicin (DOX) into nanomicelles, with high DOX loading efficiency and pH-responsive DOX release properties. The cytotoxicity, cell proliferation inhibition, endocytosis pathway, intracellular trafficking/localization, and autophagy behavior of the blank glycopolymer micelles and/or DOX-loaded micelles were studied in a Human Glioblastoma Carcinoma (H4) and green fluorescent protein-tagged H4-GFP-LC3 cell lines. The glycopolymer micelles could be taken up into the cells through favorable caveolae-mediated and clathrin-mediated endocytic pathways, and their intracellular trafficking/localization were associated with endosome-lysosome systems. Notably, after treating with DOX-loaded glycopolymer micelles (or free DOX) to the H4-GFP-LC3 cells, exogenous substances-induced autophagosome accumulation was observed. The autophagy inhibitors: 3-methyladenine (3-MA) and hydroxychloroquine (HCQ) were used to monitor the autophagy behavior of H4-GFP-LC3 cells incubated with the micelles. Interestingly, the autophagy inhibitors could significantly enhance the antitumor performance of the free DOX and/or DOX-loaded micelles, the drug combination effect of autophagy inhibitors and DOX was further studied by Bliss independent model analysis. Taken together, this work provided a preliminary understanding of the intracellular drug delivery properties of glycopolymer micelles and demonstrated the effect of different autophagy inhibitors, which might inspire future innovation of “autophagy regulator-combined nanotherapeutics” toward efficient cancer chemotherapy.
{"title":"Glycopolymer nanomicelles: pH-responsive drug delivery, endocytosis pathway, autophagy behavior, and the effect of autophagy inhibitors","authors":"Zhao Wang, Jingjing Sun, Lin Jia, Ruilong Sheng","doi":"10.1007/s10856-024-06837-4","DOIUrl":"10.1007/s10856-024-06837-4","url":null,"abstract":"<div><p>Glycopolymer drug delivery nanosystems have attracted increasing attention in the field of sustainable biomaterials and clinical biomedicine, while few studies addressed their intracellular drug delivery properties, endocytosis pathways, intracellular trafficking, autophagy behaviors and the effect of autophagy inhibitors. Based on our previous study, in this work, a pH-responsive glycopolymer (PMAgala<sub>18</sub>-<i>b</i>-P(MAA<sub>24</sub>-<i>co</i>-MAChol<sub>6</sub>)) was synthesized and used as a drug delivery carrier, to encapsulate antitumor drug doxorubicin (DOX) into nanomicelles, with high DOX loading efficiency and pH-responsive DOX release properties. The cytotoxicity, cell proliferation inhibition, endocytosis pathway, intracellular trafficking/localization, and autophagy behavior of the blank glycopolymer micelles and/or DOX-loaded micelles were studied in a Human Glioblastoma Carcinoma (H4) and green fluorescent protein-tagged H4-GFP-LC3 cell lines. The glycopolymer micelles could be taken up into the cells through favorable caveolae-mediated and clathrin-mediated endocytic pathways, and their intracellular trafficking/localization were associated with endosome-lysosome systems. Notably, after treating with DOX-loaded glycopolymer micelles (or free DOX) to the H4-GFP-LC3 cells, exogenous substances-induced autophagosome accumulation was observed. The autophagy inhibitors: 3-methyladenine (3-MA) and hydroxychloroquine (HCQ) were used to monitor the autophagy behavior of H4-GFP-LC3 cells incubated with the micelles. Interestingly, the autophagy inhibitors could significantly enhance the antitumor performance of the free DOX and/or DOX-loaded micelles, the drug combination effect of autophagy inhibitors and DOX was further studied by Bliss independent model analysis. Taken together, this work provided a preliminary understanding of the intracellular drug delivery properties of glycopolymer micelles and demonstrated the effect of different autophagy inhibitors, which might inspire future innovation of “autophagy regulator-combined nanotherapeutics” toward efficient cancer chemotherapy.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lung cancer is the leading cause of cancer deaths worldwide. Everolimus (Eve) was observed to upregulate the expression of phosphatase and tensin homolog and microRNA-4328 and inhibits the proliferation and migration of A549 cells. In the present study, a new nanocarrier based on composite containing chitosan (CS), carbon nanotubes (CNT) and ordered mesoporous (OMC) was used to load the anticancer drug everolimus (EVE). The existence of EVE on CNT/OMC/CS nanocarrier is confirmed by FE-SEM images, Raman, UV-Vis, FT-IR, BET and TGA analyses. The results showed that the introduction of CS improved the drug loading by 89.4% at pH 7.0, time 2 h and EVE to CNT/OMC/CS ratio of 1.5. Moreover, release study of EVE showed that 15.2% of EVE is released from EVE@CNT/OMC/CS at pH=7.4 for 15 h, while 78.9% of EVE is released at pH = 4.5. After 25 h, 16.8% and 88.0% of EVE were released at pH 7.4 and 4.5, respectively. Based on the MTT assay results, CNT/OMC/CS exhibited negligible cytotoxicity and good compatibility on the A549 lung cancer cell line. The cytotoxicity of the EVE@CNT/OMC/CS (IC50 of ~9 μg/mL) on the A549 cell line was higher as compared to free Eve drug (IC50 of ~13 μg/mL) after 48 h exposure time. All the data confirmed the synergistic effect of EVE in combination with CNT/OMC/CS could serve as an ideal candidate in treating lung cancer.
{"title":"Carbon nanotubes/ordered mesoporous carbon/chitosan nanocomposite as a promising carrier for everolimus targeted delivery toward lung cancer cells","authors":"Ghazal Khodarahmi, Bahare Sabeti, Fereshteh Chekin","doi":"10.1007/s10856-025-06901-7","DOIUrl":"10.1007/s10856-025-06901-7","url":null,"abstract":"<div><p>Lung cancer is the leading cause of cancer deaths worldwide. Everolimus (Eve) was observed to upregulate the expression of phosphatase and tensin homolog and microRNA-4328 and inhibits the proliferation and migration of A549 cells. In the present study, a new nanocarrier based on composite containing chitosan (CS), carbon nanotubes (CNT) and ordered mesoporous (OMC) was used to load the anticancer drug everolimus (EVE). The existence of EVE on CNT/OMC/CS nanocarrier is confirmed by FE-SEM images, Raman, UV-Vis, FT-IR, BET and TGA analyses. The results showed that the introduction of CS improved the drug loading by 89.4% at pH 7.0, time 2 h and EVE to CNT/OMC/CS ratio of 1.5. Moreover, release study of EVE showed that 15.2% of EVE is released from EVE@CNT/OMC/CS at pH=7.4 for 15 h, while 78.9% of EVE is released at pH = 4.5. After 25 h, 16.8% and 88.0% of EVE were released at pH 7.4 and 4.5, respectively. Based on the MTT assay results, CNT/OMC/CS exhibited negligible cytotoxicity and good compatibility on the A549 lung cancer cell line. The cytotoxicity of the EVE@CNT/OMC/CS (IC50 of ~9 μg/mL) on the A549 cell line was higher as compared to free Eve drug (IC50 of ~13 μg/mL) after 48 h exposure time. All the data confirmed the synergistic effect of EVE in combination with CNT/OMC/CS could serve as an ideal candidate in treating lung cancer.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diabetes mellitus, a persistent metabolic disorder characterized by elevated blood glucose levels, presents a substantial global health challenge due to its escalating prevalence and associated complications. In the current study, a nanofibrous scaffold was developed for the co-delivery of bone marrow-derived stem cells and Euphorbia hirta extract into the diabetic wound beds. Scaffolds were produced using the electrospinning method, and their physicochemical and biological properties were studied in vitro. The wound healing function of the developed delivery system was investigated in a rat model of diabetic wound healing. The study showed that Euphorbia hirta extract-loaded scaffolds imparted no significant toxicity toward L929 cells and promoted their migration activity. A wound healing study showed that scaffolds loaded with both bone marrow-derived stem cells and euphorbia hirta extract had the highest wound healing activity, as shown by significantly higher wound closure percentage, collagen deposition, and epithelial thickness. Enzyme-linked immunosorbent Assay studies showed that these dressings reduced tissue oxidative stress and alleviated inflammatory responses.
{"title":"Diabetic wound healing via a co-delivery system for bone marrow derived stem cells and euphorbia hirta extract: an in vitro and in vivo study","authors":"Jiazhang Duan, Yun Yao, Jiafei Wang, Wuhua Liu, Saeed Rohani, Huagang Yang","doi":"10.1007/s10856-025-06880-9","DOIUrl":"10.1007/s10856-025-06880-9","url":null,"abstract":"<div><p>Diabetes mellitus, a persistent metabolic disorder characterized by elevated blood glucose levels, presents a substantial global health challenge due to its escalating prevalence and associated complications. In the current study, a nanofibrous scaffold was developed for the co-delivery of bone marrow-derived stem cells and Euphorbia hirta extract into the diabetic wound beds. Scaffolds were produced using the electrospinning method, and their physicochemical and biological properties were studied in vitro. The wound healing function of the developed delivery system was investigated in a rat model of diabetic wound healing. The study showed that Euphorbia hirta extract-loaded scaffolds imparted no significant toxicity toward L929 cells and promoted their migration activity. A wound healing study showed that scaffolds loaded with both bone marrow-derived stem cells and euphorbia hirta extract had the highest wound healing activity, as shown by significantly higher wound closure percentage, collagen deposition, and epithelial thickness. Enzyme-linked immunosorbent Assay studies showed that these dressings reduced tissue oxidative stress and alleviated inflammatory responses.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-025-06880-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The metal-based devices may corrode, degrade, or release metal ions and fragments after being implanted in the body, exhibiting their own consequences on hosting organs/tissues. The biocompatibility of metal implants has been investigated in various studies using a number of cell types. Mesenchymal stem cells (MSCs) are more relevant cells than others for evaluating the cytocompatibility of metal-based orthopedic implants because they are essential cells for bone regeneration and a promising cell population in regenerative medicine. In this regard, stemness preservation of MSCs is a key property in both body’s own repair process and success of renewing/compensating approaches. In general, MSCs adhesion, viability, and function at the cell–metal interface is directly dependent on the metal alloys composing elements, which, along with consideration of compatibility, could guarantee the success of implants. This review scrutinizes the effects of orthopedic metal materials on the biocompatibility and stemness of MSCs at metal interface. Additionally, in vivo, host responses to metal implants are investigated.
{"title":"An update on the effect of metals on stemness properties of mesenchymal stem cells","authors":"Roya Valizadeh, Halimeh Amirazad, Tahura Fayeghi, Hanieh Mousazadeh, Nosratollah Zarghami, Abbas Ebrahimi-Kalan, Effat Alizadeh","doi":"10.1007/s10856-025-06865-8","DOIUrl":"10.1007/s10856-025-06865-8","url":null,"abstract":"<div><p>The metal-based devices may corrode, degrade, or release metal ions and fragments after being implanted in the body, exhibiting their own consequences on hosting organs/tissues. The biocompatibility of metal implants has been investigated in various studies using a number of cell types. Mesenchymal stem cells (MSCs) are more relevant cells than others for evaluating the cytocompatibility of metal-based orthopedic implants because they are essential cells for bone regeneration and a promising cell population in regenerative medicine. In this regard, stemness preservation of MSCs is a key property in both body’s own repair process and success of renewing/compensating approaches. In general, MSCs adhesion, viability, and function at the cell–metal interface is directly dependent on the metal alloys composing elements, which, along with consideration of compatibility, could guarantee the success of implants. This review scrutinizes the effects of orthopedic metal materials on the biocompatibility and stemness of MSCs at metal interface. Additionally, in vivo, host responses to metal implants are investigated.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-025-06865-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-05-20DOI: 10.1007/s10856-025-06896-1
Jiahao Xu, Linyu Long, Xiaoyu Zhou, Xinyue Zhang, Li Liao, Dan Ji, Xuanchu Duan
Brinzolamide is a widely used treatment for glaucoma, but its effectiveness relies on at least twice-daily dosing, which can be challenging for patient adherence. To overcome this limitation, we developed an injectable hydrogel-based delivery system designed to maintain therapeutic drug levels with a single administration. This approach aims to simplify treatment and improve clinical outcomes. Brinzolamide-loaded polyethylene glycol poly (lactic-co-glycolic acid) (PEG-PLGA) nanoparticles were encapsulated within a hydrogel synthesized through the crosslinking of oxidized hyaluronic acid (OHA) and carboxymethyl chitosan (CMC). In vitro studies were conducted to assess the nanoparticles’ characterization, release profile, and biocompatibility. In a steroid-induced high intraocular pressure (IOP) mouse model, the efficacy of a single subconjunctival injection in lowering IOP was evaluated. Additionally, both cellular and animal biocompatibility were assessed. The brinzolamide-loaded hydrogel system (Hydrogel@Brz) contained nanoparticles with an average diameter of 40.76 nm, exhibiting a stable size distribution and a spherical morphology. The hydrogel demonstrated excellent injectability, self-healing properties, and a porous structure conducive to nanoparticle encapsulation. In vitro release studies revealed a sustained drug release of 86% over 14 days. No cytotoxicity was observed in human primary trabecular meshwork cells (HTMCs), human Tenon’s capsule fibroblasts (HTFs), or the retinal ganglion cell line R28. In vivo, a single injection led to a prolonged IOP reduction lasting up to 21 days. No signs of drug toxicity were detected in ocular tissue sections, transverse optic nerve sections under transmission electron microscopy, or pathology slides of various organs. The brinzolamide-loaded hydrogel has demonstrated promising potential for sustained drug delivery and effective intraocular pressure reduction while maintaining good biocompatibility. However, further studies in larger animal models and long-term evaluations are needed to confirm its clinical applicability.
{"title":"Sustained intraocular pressure-lowering effect and biocompatibility of a single subconjunctival administration of hydrogel-encapsulated nano-brinzolamide","authors":"Jiahao Xu, Linyu Long, Xiaoyu Zhou, Xinyue Zhang, Li Liao, Dan Ji, Xuanchu Duan","doi":"10.1007/s10856-025-06896-1","DOIUrl":"10.1007/s10856-025-06896-1","url":null,"abstract":"<div><p>Brinzolamide is a widely used treatment for glaucoma, but its effectiveness relies on at least twice-daily dosing, which can be challenging for patient adherence. To overcome this limitation, we developed an injectable hydrogel-based delivery system designed to maintain therapeutic drug levels with a single administration. This approach aims to simplify treatment and improve clinical outcomes. Brinzolamide-loaded polyethylene glycol poly (lactic-co-glycolic acid) (PEG-PLGA) nanoparticles were encapsulated within a hydrogel synthesized through the crosslinking of oxidized hyaluronic acid (OHA) and carboxymethyl chitosan (CMC). In vitro studies were conducted to assess the nanoparticles’ characterization, release profile, and biocompatibility. In a steroid-induced high intraocular pressure (IOP) mouse model, the efficacy of a single subconjunctival injection in lowering IOP was evaluated. Additionally, both cellular and animal biocompatibility were assessed. The brinzolamide-loaded hydrogel system (Hydrogel@Brz) contained nanoparticles with an average diameter of 40.76 nm, exhibiting a stable size distribution and a spherical morphology. The hydrogel demonstrated excellent injectability, self-healing properties, and a porous structure conducive to nanoparticle encapsulation. In vitro release studies revealed a sustained drug release of 86% over 14 days. No cytotoxicity was observed in human primary trabecular meshwork cells (HTMCs), human Tenon’s capsule fibroblasts (HTFs), or the retinal ganglion cell line R28. In vivo, a single injection led to a prolonged IOP reduction lasting up to 21 days. No signs of drug toxicity were detected in ocular tissue sections, transverse optic nerve sections under transmission electron microscopy, or pathology slides of various organs. The brinzolamide-loaded hydrogel has demonstrated promising potential for sustained drug delivery and effective intraocular pressure reduction while maintaining good biocompatibility. However, further studies in larger animal models and long-term evaluations are needed to confirm its clinical applicability.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-025-06896-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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