Journal of Materials Science: Materials in Medicine最新文献_第6页

Si and Zn dual ions upregulate the osteogenic differentiation of mBMSCs: mRNA transcriptomic sequencing analysis 硅和锌双离子上调 mBMSCs 的成骨分化：mRNA 转录组测序分析

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-09-10 DOI: 10.1007/s10856-024-06825-8

Xinyuan Yuan, Tingting Wu, Teliang Lu, Jiandong Ye

Both silicon (Si) and zinc (Zn) ions are essential elements to bone health and their mechanisms for promoting osteogenesis have aroused the extensive attention of researchers. Thereinto, the mechanism by which dual ions promote osteogenic differentiation remains to be elucidated. Herein, the effects of Si and Zn ions on the cytological behaviors of mBMSCs were firstly studied. Then, the molecular mechanism of Si-Zn dual ions regulating the osteogenic differentiation of mBMSCs was investigated via transcriptome sequencing technology. In the single-ion system, Si ion at the concentration of 1.5 mM (Si-1.5) had better comprehensive effects of cell proliferation, ALP activity and osteogenesis-related gene expression levels (ALP, Runx2, OCN, Col-I and BSP); Zn ion at the concentration of 50 μM (Zn-50) demonstrated better combining effects of cell proliferation, ALP activity and same osteogenic genes expression levels. In the dual-ion system, the Si (1.5 mM)-Zn (50 μM) group (Si1.5-Zn50) synthetically enhanced ALP activity and osteogenesis genes compared with single-ion groups. Analysis of the transcriptome sequencing results showed that Si ion had a certain effect on promoting the osteogenic differentiation of mBMSCs; Zn ion had a stronger effect of contributing to a better osteogenic differentiation of mBMSCs than that of Si ion; the Si-Zn dual ions had a synergistic enhancement on conducting to the osteogenic differentiation of mBMSCs compared to single ion (Si or Zn). This study offers a blueprint for exploring the regulation mechanism of osteogenic differentiation by dual ions.

Graphical Abstract

硅（Si）和锌（Zn）离子都是骨骼健康的重要元素，它们促进成骨的机制引起了研究人员的广泛关注。然而，双离子促进成骨分化的机制仍有待阐明。本文首先研究了 Si 和 Zn 离子对 mBMSCs 细胞学行为的影响。然后，通过转录组测序技术研究了Si-Zn双离子调控mBMSCs成骨分化的分子机制。在单离子体系中，浓度为1.5 mM（Si-1.5）的Si离子对细胞增殖、ALP活性和成骨相关基因（ALP、Runx2、OCN、Col-I和BSP）表达水平有较好的综合效应；浓度为50 μM（Zn-50）的Zn离子对细胞增殖、ALP活性和相同的成骨基因表达水平有较好的综合效应。在双离子系统中，与单离子组相比，Si（1.5 mM）-Zn（50 μM）组（Si1.5-Zn50）能合成性地提高 ALP 活性和成骨基因。转录组测序分析结果表明，硅离子对促进 mBMSCs 成骨分化有一定作用；与硅离子相比，锌离子对促进 mBMSCs 成骨分化的作用更强；与单离子（硅或锌）相比，硅锌双离子对促进 mBMSCs 成骨分化有协同增强作用。该研究为探索双离子对成骨分化的调控机制提供了蓝图。图文摘要

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引用次数: 0

Three-dimensional printed calcium phosphate scaffolds emulate bone microstructure to promote bone regrowth and repair 三维打印磷酸钙支架可模拟骨骼微观结构，促进骨骼再生和修复。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-09-03 DOI: 10.1007/s10856-024-06817-8

Kyohei Takase, Takahiro Niikura, Tomoaki Fukui, Yohei Kumabe, Kenichi Sawauchi, Ryo Yoshikawa, Yuya Yamamoto, Ryota Nishida, Tomoyuki Matsumoto, Ryosuke Kuroda, Keisuke Oe

The interconnected structures in a 3D scaffold allows the movement of cells and nutrients. Therefore, this study aimed to investigate the in-vivo bioactivity of 3D-printed β-tricalcium phosphate (β-TCP) and hydroxyapatite (HAP) scaffolds that replicate biological bone. This study included 24-week-old male New Zealand white rabbits. A cylindrical bone defect with a diameter of 4.5 mm and a depth of 8 mm was created in the lateral aspect of the distal femur. A 3D-printed scaffold was implanted in the right femur (experimental side), whereas the left femur was kept free of implantation (control side). Micro-CT analysis and histological observations of the bone defect site were conducted at 4, 8, and 12 weeks postoperatively to track the bone repair progress. No evidence of new bone tissue formation was found in the medullary cavity of the bone defect on the control side. In contrast, on the experimental side, the 3D scaffold demonstrated sufficient bioactivity, leading to the growth of new bone tissue. Over time, new bone tissue gradually extended from the periphery toward the center, a phenomenon evident in both micro-CT images and biopsy staining. In the current study, we observed that the cells involved in bone metabolism adhered, spread, and proliferated on our newly designed 3D-printed scaffold with a bone microstructure. Therefore, it is suggested that this scaffold has sufficient bioactivity to induce new bone formation and could be expected to be a more useful artificial bone than the existing version.

Graphical Abstract

三维支架中相互连接的结构允许细胞和营养物质的移动。因此，本研究旨在调查三维打印的β-磷酸三钙（β-TCP）和羟基磷灰石（HAP）支架在体内复制生物骨骼的生物活性。这项研究包括 24 周大的雄性新西兰白兔。在股骨远端外侧创建了一个直径为 4.5 毫米、深度为 8 毫米的圆柱形骨缺损。在右侧股骨（实验侧）植入 3D 打印支架，左侧股骨（对照侧）则不植入支架。术后4周、8周和12周，对骨缺损部位进行了显微CT分析和组织学观察，以跟踪骨修复的进展情况。对照组一侧骨缺损的髓腔内没有发现新骨组织形成的迹象。相反，在实验侧，三维支架表现出了足够的生物活性，导致了新骨组织的生长。随着时间的推移，新骨组织逐渐从外围向中心延伸，这一现象在显微 CT 图像和活检染色中都很明显。在本研究中，我们观察到参与骨代谢的细胞在我们新设计的具有骨微结构的三维打印支架上附着、扩散和增殖。因此，这种支架具有足够的生物活性来诱导新骨形成，有望成为比现有版本更有用的人工骨。

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引用次数: 0

In vitro evaluation of the biocompatibility and bioactivity of a SLM-fabricated NiTi alloy with superior tensile property 对具有优异拉伸性能的 SLM 制造镍钛合金的生物相容性和生物活性进行体外评估。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-08-23 DOI: 10.1007/s10856-024-06822-x

Yu Sun, Zhihui Zhang, Qingping Liu, Luquan Ren, Jincheng Wang

Because nickel-titanium (NiTi) alloys have unique functions, such as superelasticity, shape memory, and hysteresis similar to bone in the loading-unloading cycles of their recoverable deformations. They likely offer good bone integration, a low loosening rate, individual customization, and ease of insertion. Due to the poor processability of NITI, traditional methods cannot manufacture NiTi products with complex shapes. Orthopedic NiTi implants need to show an adequate fracture elongation of at least 8%. Additive manufacturing can be used to prepare NiTi implants with complex structures and tunable porosity. However, as previously reported, additively manufactured NiTi alloys could only exhibit a maximum tensile fracture strain of 7%. In new reports, a selective laser melting (SLM)–NiTi alloy has shown greater tensile strain (15.6%). Nevertheless, due to the unique microstructure of additive manufacturing NiTi that differs from traditional NITI, the biocompatibility of SLM-NITI manufactured by this new process requires further evaluation In this study, the effects of the improved NiTi alloy on bone marrow mesenchymal stem cell (BMSC) proliferation, adhesion, and cell viability were investigated via in vitro studies. A commercial Ti-6Al-4V alloy was studied side-by-side for comparison. Like the Ti-6Al-4V alloy, the SLM-NiTi alloy exhibited low cytotoxicity toward BMSCs and similar effect on cell adhesion or cell viability. This study demonstrates that the new SLM-NiTi alloy, which has exhibited improved mechanical properties, also displays excellent biocompatibility. Therefore, this alloy may be a superior implant material in biomedical implantation.

Graphical Abstract

由于镍钛（NiTi）合金具有独特的功能，如超弹性、形状记忆以及在其可恢复变形的加载-卸载循环中与骨骼相似的滞后性。它们可能具有良好的骨整合性、低松动率、个性化定制和易于插入等特点。由于镍钛材料的加工性较差，传统方法无法制造形状复杂的镍钛产品。骨科镍钛植入物需要有足够的断裂伸长率，至少为 8%。增材制造可用于制备具有复杂结构和可调孔隙率的镍钛植入体。然而，根据之前的报道，添加剂制造的镍钛合金只能表现出 7% 的最大拉伸断裂应变。在新的报道中，一种选择性激光熔化（SLM）-镍钛合金显示出更大的拉伸应变（15.6%）。本研究通过体外研究调查了改进型镍钛合金对骨髓间充质干细胞（BMSC）增殖、粘附和细胞活力的影响。同时还对商用 Ti-6Al-4V 合金进行了对比研究。与 Ti-6Al-4V 合金一样，SLM-NiTi 合金对 BMSCs 的细胞毒性较低，对细胞粘附性或细胞活力的影响相似。这项研究表明，新型 SLM-NiTi 合金不仅具有更好的机械性能，还具有出色的生物相容性。因此，这种合金可能是生物医学植入中的一种优质植入材料。

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引用次数: 0

TiO2-ZnPc nanoparticles functionalized with folic acid as a target photosensitizer for photodynamic therapy against glioblastoma cells 叶酸功能化 TiO2-ZnPc 纳米粒子作为光动力疗法的靶光敏剂，用于治疗胶质母细胞瘤细胞。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-08-22 DOI: 10.1007/s10856-024-06823-w

Gustavo Jardón-Guadarrama, Ma Elena Manríquez-Ramírez, Citlali E. Rodríguez-Pérez, Araceli Díaz-Ruiz, María de los Ángeles Martínez-Cárdenas, Alfonso Mata-Bermudez, Camilo Ríos, Emma Ortiz-Islas

The use of TiO₂ as a photosensitizer in photodynamic therapy is limited due to TiO₂ generates reactive oxygen species only under UV irradiation. The TiO₂ surface has been modified with different functional groups to achieve activation at longer wavelengths (visible light). This work reports the synthesis, characterization, and biological toxicity assay of TiO₂ nanoparticles functionalized with folic acid and combined with a zinc phthalocyanine to obtain a nano-photosensitizer for its application in photodynamic therapy for glioblastoma cancer treatment. The nano-photosensitizer was prepared using the sol-gel method. Folic acid and zinc phthalocyanine were added during the hydrolysis and condensation of titanium butoxide, which was the TiO₂ precursor. The samples obtained were characterized by several microscopy and spectroscopy techniques. An in vitro toxicity test was performed using the MTT assay and the C6 cellular line. The results of the characterization showed that the structure of the nanoparticles corresponds mainly to the anatase phase. Successful functionalization with folic acid and an excellent combination with phthalocyanine was also achieved. Both folic acid-functionalized TiO₂ and phthalocyanine-functionalized TiO₂ had no cytotoxic effect on C6 cells (even at high concentrations) in comparison to Cis-Pt, which was very toxic to C6 cells. The materials behaved similarly to the control (untreated cells). The cell viability and light microscopy images suggest that both materials could be considered biocompatible and mildly phototoxic in these cells when activated by light.

Graphical Abstract

由于二氧化钛只能在紫外线照射下产生活性氧，因此在光动力疗法中使用二氧化钛作为光敏剂受到了限制。为了在更长的波长（可见光）下实现活化，TiO2 的表面已被不同的官能团修饰。本研究报告了叶酸功能化 TiO2 纳米粒子的合成、表征和生物毒性检测，并结合酞菁锌获得了一种纳米光敏剂，可应用于治疗胶质母细胞瘤的光动力疗法。该纳米光敏剂采用溶胶-凝胶法制备。在作为 TiO2 前体的丁氧化钛的水解和缩合过程中加入了叶酸和酞菁锌。获得的样品通过多种显微镜和光谱技术进行了表征。使用 MTT 试验和 C6 细胞系进行了体外毒性测试。表征结果表明，纳米颗粒的结构主要对应锐钛矿相。此外，还成功实现了与叶酸的功能化以及与酞菁的完美结合。叶酸功能化的二氧化钛和酞菁功能化的二氧化钛对 C6 细胞都没有细胞毒性作用（即使在高浓度下），而顺式铂则对 C6 细胞有剧毒。这些材料的表现与对照组（未处理的细胞）相似。细胞存活率和光学显微镜图像表明，这两种材料都具有生物相容性，在光的激活下对这些细胞具有轻微的光毒性。

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引用次数: 0

Preparation of composite calcium phosphate cement scaffold loaded with Hedysarum polysaccharides and its efficacy in repairing bone defects 负载 Hedysarum 多糖的复合磷酸钙水泥支架的制备及其在修复骨缺损中的功效。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-08-13 DOI: 10.1007/s10856-024-06818-7

Lianggong Zhao, Bo Wang, Shilan Feng, Huifang Wu

It’s imperative to create a more ideal biological scaffold for bone defect repair. Calcium phosphate bone cements (CPC) could be used as a scaffold. Some ingredients and osteogenic factors could be added to improve its poor mechanical properties and biological activity. As a macromolecule extracted from traditional Chinese medicine, Hedysarum polysaccharides (HPS) would significantly promote the osteogenic activity of bone biomaterials. Zirconium oxide and starch were added to the solid phase and citric acid was added to the liquid phase to optimize CPC. HPS was loaded onto the scaffold as an osteogenic factor, and the prepared CPS + HPS was characterized. Further, the cytocompatibility of CPS + HPS was assessed according to activity, differentiation, and calcification in neonatal rat calvarial osteoblasts, and the biosafety of CPS + HPS was evaluated according to acute toxicity, pyrogen, sensitization, and hemolysis. The success of CPS + HPS in repairing bone defects was evaluated by using a rabbit femur implantation experiment. After optimization, CPS-20-CA-5 containing 10% starch and 5% citric acid displayed the highest mechanical strength of 28.96 ± 0.03 MPa. HPS-50 was demonstrated to exert the best osteogenic effect. The combination of CPS + HPS achieved HPS-loaded CPC. Material characterization, cytocompatibility, biosafety, and femoral implantation experiments indicated that CPS + HPS possessed better pressure resistance and improved osteogenic ability in bone defect repair.CPS + HPS demonstrated effective pressure resistance and superior osteogenic ability, which may be of great significance for bone defects and bone tissue engineering to promote bone regeneration and repair.

当务之急是为骨缺损修复创造一种更理想的生物支架。磷酸钙骨水泥（CPC）可用作支架。可以添加一些成分和成骨因子来改善其不良的机械性能和生物活性。作为一种从传统中药中提取的大分子，海带多糖（HPS）可显著促进骨生物材料的成骨活性。在固相中加入氧化锆和淀粉，在液相中加入柠檬酸以优化 CPC。将 HPS 作为成骨因子添加到支架上，并对制备的 CPS + HPS 进行了表征。此外，还根据新生大鼠小腿成骨细胞的活性、分化和钙化情况评估了 CPS + HPS 的细胞相容性，并根据急性毒性、热原、致敏和溶血情况评估了 CPS + HPS 的生物安全性。通过兔股骨植入实验评估了 CPS + HPS 修复骨缺损的成功率。经过优化，含有 10%淀粉和 5%柠檬酸的 CPS-20-CA-5 显示出最高的机械强度（28.96 ± 0.03 兆帕）。HPS-50 的成骨效果最佳。CPS + HPS的组合实现了HPS负载CPC。材料表征、细胞相容性、生物安全性和股骨植入实验表明，CPS + HPS 在骨缺损修复中具有更好的抗压性和更强的成骨能力。

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引用次数: 0

Assessment of cranial reconstruction utilizing various implant materials: finite element study 利用各种植入材料进行颅骨重建的评估：有限元研究。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-08-13 DOI: 10.1007/s10856-024-06816-9

Yomna H. Shash

The human head can sometimes experience impact loads that result in skull fractures or other injuries, leading to the need for a craniectomy. Cranioplasty is a procedure that involves replacing the removed portion with either autologous bone or alloplastic material. While titanium has traditionally been the preferred material for cranial implants due to its excellent properties and biocompatibility, its limitations have prompted the search for alternative materials. This research aimed to explore alternative materials to titanium for cranial implants in order to address the limitations of titanium implants and improve the performance of the cranioplasty process. A 3D model of a defective skull was reconstructed with a cranial implant, and the implant was simulated using various stiff and soft materials (such as alumina, zirconia, hydroxyapatite, zirconia-reinforced PMMA, and PMMA) as alternatives to titanium under 2000N impact forces. Alumina and zirconia implants were found to reduce stresses and strains on the skull and brain compared to titanium implants. However, PMMA implants showed potential for causing skull damage under current loading conditions. Additionally, PMMA and hydroxyapatite implants were prone to fracture. Despite these findings, none of the implants exceeded the limits for tensile and compressive stresses and strains on the brain. Zirconia-reinforced PMMA implants were also shown to reduce stresses and strains on the skull and brain compared to PMMA implants. Alumina and zirconia show promise as alternatives to titanium for the production of cranial implants. The use of alternative implant materials to titanium has the potential to enhance the success of cranial reconstruction by overcoming the limitations associated with titanium implants.

Graphical Abstract

人的头部有时会受到冲击负荷，导致颅骨骨折或其他损伤，从而需要进行颅骨切除术。颅骨成形术是一种用自体骨或异体材料替代切除部分的手术。虽然钛因其优异的性能和生物相容性一直是颅骨植入物的首选材料，但其局限性促使人们寻找替代材料。本研究旨在探索用于颅骨植入物的钛替代材料，以解决钛植入物的局限性，提高颅骨成形术的性能。研究人员用颅骨植入体重建了一个有缺陷的颅骨三维模型，并使用各种软硬材料（如氧化铝、氧化锆、羟基磷灰石、氧化锆增强聚甲基丙烯酸甲酯和聚甲基丙烯酸甲酯）模拟了植入体在 2000N 冲击力下替代钛的情况。与钛植入物相比，氧化铝和氧化锆植入物可减少头骨和大脑的应力和应变。不过，PMMA 植入物显示在当前加载条件下有可能造成颅骨损伤。此外，PMMA 和羟基磷灰石植入物容易发生断裂。尽管有这些发现，但没有一个植入物超过大脑的拉伸和压缩应力和应变极限。与 PMMA 植入体相比，氧化锆增强 PMMA 植入体还能减少头骨和大脑的应力和应变。氧化铝和氧化锆有望成为钛的替代品，用于生产颅骨植入物。使用钛的替代植入体材料有可能克服钛植入体的局限性，从而提高颅骨重建的成功率。

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引用次数: 0

Feasibility of using diamond-like carbon films in total joint replacements: a review 在全关节置换术中使用类金刚石碳膜的可行性：综述。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-08-13 DOI: 10.1007/s10856-024-06814-x

Anurag Roy, Annette Bennett, Lisa Pruitt

Diamond-like Carbon (DLC) has been used as a coating material of choice for a variety of technological applications owing to its favorable bio-tribo-thermo-mechanical characteristics. Here, the possibility of bringing DLC into orthopedic joint implants is examined. With ever increasing number of patients suffering from osteoarthritis as well as with the ingress of the osteoarthritic joints’ malaise into younger and more active demographics, there is a pressing need to augment the performance and integrity of conventional total joint replacements (TJRs). Contemporary joint replacement devices use metal-on-polymer articulations to restore function to worn, damaged or diseased cartilage. The wear of polymeric components has been addressed using crosslinking and antioxidants; however, in the context of the metallic components, complications pertaining to corrosion and metal ion release inside the body still persist. Through this review article, we explore the use of DLC coatings on metallic bearing surfaces and elucidate why this technology might be a viable solution for ongoing electrochemical challenges in orthopedics. The different characteristics of DLC coatings and their feasibility in TJRs are examined through assessment of tribo-material characterization methods. A holistic characterization of the coating-substrate interface and the wear performance of such systems are discussed. As with all biomaterials used in TJRs, we need mindful consideration of potential in-vivo challenges. We present a few caveats for DLC coatings including delamination, hydrophobicity, and other conflicting as well as outdating findings in the literature. We recommend prudently exploring DLC films as potential coatings on metallic TJR components to solve the problems pertaining to wear, metal ion release, and corrosion. Ultimately, we advise bringing DLC into clinical use only after addressing all challenges and concerns outlined in this article.

Graphical Abstract

类金刚石碳（DLC）具有良好的生物三热力学特性，已被用作各种技术应用的首选涂层材料。本文将探讨将 DLC 应用于骨科关节植入物的可能性。随着骨关节炎患者人数的不断增加，以及骨关节炎患者逐渐年轻化和活跃化，迫切需要提高传统全关节置换术（TJR）的性能和完整性。当代关节置换装置使用金属聚合物关节来恢复磨损、受损或病变软骨的功能。聚合物组件的磨损问题已通过交联和抗氧化剂得到解决；但在金属组件方面，与腐蚀和体内金属离子释放有关的并发症仍然存在。通过这篇综述文章，我们探讨了在金属轴承表面使用 DLC 涂层的问题，并阐明了为什么这项技术可能成为解决整形外科电化学难题的可行方案。通过对三材料表征方法的评估，研究了 DLC 涂层的不同特性及其在 TJR 中的可行性。还讨论了涂层-基底界面的整体表征以及此类系统的磨损性能。与所有用于 TJR 的生物材料一样，我们需要认真考虑潜在的体内挑战。我们提出了 DLC 涂层的一些注意事项，包括分层、疏水性以及文献中其他相互矛盾和过时的研究结果。我们建议审慎探索将 DLC 薄膜作为 TJR 金属组件的潜在涂层，以解决磨损、金属离子释放和腐蚀等相关问题。最后，我们建议只有在解决了本文概述的所有挑战和问题后，才能将 DLC 用于临床。

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引用次数: 0

Improvement photothermal property of MoS2/Fe3O4/GNR nanocomposite in cancer treatment 改善 MoS2/Fe3O4/GNR 纳米复合材料在癌症治疗中的光热特性。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-08-13 DOI: 10.1007/s10856-024-06819-6

Behdad Shariati, Mohammad Taghi Goodarzi, Alireza Jalali, Nasrin Salehi, Majid Mozaffari

The objective of the present study was to develop a novel molybdenum disulfide/iron oxide/gold nanorods (MoS₂/Fe₃O₄/GNR) nanocomposite (MFG) with different concentrations of AgNO₃ solution (MFG1, MFG2, and MFG3) for topical doxorubicin (DOX) drug delivery. Then, these nanocomposites were synthesized and characterized by Fourier transform infrared (FTIR), Transmission electron microscopy (TEM), Dynamic light scattering (DLS), and Ultraviolet-visible (UV–Vis) spectroscopies to confirm their structural and optical properties. Cytotoxicity of samples on Hela cell was determined using MTT assay. Results indicated that nanocomposites possess little cytotoxicity without NIR laser irradiation. Also, the relative viabilities of Hela cells decreased when the concentration of AgNO₃ solution increased in this nanocomposite. Using NIR irradiation, the relative viabilities of Hela cells decreased when the concentration of samples increased. Acridine orange/propidium iodide (PI) staining, flow cytometry were recruited to evaluate the effect of these nanocomposites on apoptosis of Hela cells. Finally, results revealed when DOX loading increased in nanocomposite, then cell viability was decreased in it. Therefore, these properties make MFG3 nanocomposite a good candidate for photothermal therapy and drug loading.

Graphical Abstract

本研究旨在开发一种新型二硫化钼/氧化铁/金纳米棒（MoS2/Fe3O4/GNR）纳米复合材料（MFG），并将其与不同浓度的 AgNO3 溶液（MFG1、MFG2 和 MFG3）混合，用于局部给药多柔比星（DOX）。然后，合成了这些纳米复合材料，并通过傅立叶变换红外光谱（FTIR）、透射电子显微镜（TEM）、动态光散射（DLS）和紫外可见光谱（UV-Vis）对其进行表征，以确认其结构和光学特性。使用 MTT 试验测定了样品对 Hela 细胞的细胞毒性。结果表明，在没有近红外激光照射的情况下，纳米复合材料的细胞毒性很小。此外，当该纳米复合材料中的 AgNO3 溶液浓度增加时，Hela 细胞的相对活力降低。使用近红外激光照射时，当样品浓度增加时，Hela 细胞的相对存活率降低。利用吖啶橙/碘化丙啶（PI）染色法和流式细胞术评估了这些纳米复合材料对 Hela 细胞凋亡的影响。结果表明，当纳米复合材料中的 DOX 负荷增加时，细胞活力降低。因此，这些特性使 MFG3 纳米复合材料成为光热疗法和药物负载的良好候选材料。

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引用次数: 0

Prolonged retention of luliconazole nanofibers for topical mycotic condition: development, in vitro characterization and antifungal activity against Candida albicans 用于局部真菌病的氟环唑纳米纤维的长期保留：开发、体外表征和对白色念珠菌的抗真菌活性。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-08-08 DOI: 10.1007/s10856-024-06815-w

Akashkumar Doshi, Bala Prabhakar, Sarika Wairkar

An antifungal agent, luliconazole, is commercially available in cream or gel form. The major limitation of these conventional formulations is less residence time at the infection site. The primary objective of this work was to develop luliconazole-loaded polyvinyl alcohol (Luz-PVA) nanofibers for mycotic skin conditions with a longer retention. Luz-PVA nanofibers were prepared by plate electrospinning and optimized for polymer concentration and process parameters. The optimized batch (Trial 5) was prepared by 10% PVA, processed at 22.4 kV applied voltage, and 14 cm plate and spinneret distance to yield thick, uniform, and peelable nanofibers film. There was no interaction observed between Luz and PVA in the FTIR study. DSC and XRD analysis showed that luliconazole was loaded into fabricated nanofibers with a reduced crystallinity. FESEM studies confirmed the smooth, defect-free mats of nanofibers. Luz-PVA nanofibers possessed a tensile strength of 21.8 N and a maximum elongation of 10.8%, representing the excellent elasticity of the scaffolds. For Luz-PVA nanofibers, the sustained and complete drug release was observed in 48 h. In antifungal activity using Candida albicans, the Luz-PVA nanofibers showed a greater zone of inhibition (30.55 ± 0.38 mm and 29.27 ± 0.31 mm) than marketed cream (28.06 ± 0.18 mm and 28.47 ± 0.24 mm) and pure drug (27.57 ± 0.17 mm and 27.50 ± 0.47 mm) at 1% concentration in Sabouraud dextrose agar and yeast malt agar, respectively. Therefore, Luz-PVA nanofibers exhibited good mechanical properties, longer retention time, and better antifungal activity than marketed products and, therefore, can be further examined preclinically as a potential treatment option for topical mycotic infection.

Graphical Abstract

市面上有乳膏或凝胶状的抗真菌剂氟硅唑。这些传统制剂的主要局限是在感染部位的停留时间较短。这项工作的主要目的是开发出用于真菌性皮肤病的具有较长滞留时间的含氟硅唑的聚乙烯醇（Luz-PVA）纳米纤维。通过平板电纺制备了 Luz-PVA 纳米纤维，并对聚合物浓度和工艺参数进行了优化。优化批次（试验 5）采用 10%的 PVA 制备，在 22.4 kV 的应用电压和 14 cm 的板和喷丝板间距下进行处理，以获得厚实、均匀和可剥离的纳米纤维膜。在傅立叶变换红外光谱研究中没有观察到 Luz 和 PVA 之间的相互作用。DSC 和 XRD 分析表明，卢立康唑被负载到制成的纳米纤维中，结晶度降低。FESEM 研究证实了纳米纤维光滑、无缺陷。Luz-PVA 纳米纤维的拉伸强度为 21.8 N，最大伸长率为 10.8%，表明支架具有良好的弹性。在使用白色念珠菌进行抗真菌活性试验时，Luz-PVA 纳米纤维的抑菌区（30.55 ± 0.38 mm 和 29.27 ± 0.31 mm）大于市场上销售的乳膏（29.27 ± 0.31 mm）。31 mm），高于市售乳膏（28.06 ± 0.18 mm 和 28.47 ± 0.24 mm）和纯药物（27.57 ± 0.17 mm 和 27.50 ± 0.47 mm）。因此，与市售产品相比，Luz-PVA 纳米纤维具有良好的机械性能、更长的保留时间和更好的抗真菌活性，因此可作为局部真菌感染的潜在治疗方案进行进一步的临床前研究。

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引用次数: 0

Biomaterial Fg/P(LLA-CL) regulates macrophage polarization and recruitment of mesenchymal stem cells after endometrial injury 生物材料Fg/P(LLA-CL)可调节巨噬细胞极化和子宫内膜损伤后间充质干细胞的招募。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-07-29 DOI: 10.1007/s10856-024-06807-w

Sirui Song, Anfeng Wang, Siyu Wu, Huaifang Li, Hongbing He

The process of endometrial repair after injury involves the synergistic action of various cells including immune cells and stem cells. In this study, after combing Fibrinogen(Fg) with poly(L-lacticacid)-co-poly(ε-caprolactone)(P(LLA-CL)) by electrospinning, we placed Fg/P(LLA-CL) into the uterine cavity of endometrium-injured rats, and bioinformatic analysis revealed that Fg/P(LLA-CL) may affect inflammatory response and stem cell biological behavior. Therefore, we verified that Fg/P(LLA-CL) could inhibit the lipopolysaccharide (LPS)-stimulated macrophages from switching to the pro-inflammatory M1 phenotype in vitro. Moreover, in the rat model of endometrial injury, Fg/P(LLA-CL) effectively promoted the polarization of macrophages towards the anti-inflammatory M2 phenotype and enhanced the presence of mesenchymal stem cells at the injury site. Overall, Fg/P(LLA-CL) exhibits significant influence on macrophage polarization and stem cell behavior in endometrial injury, justifying further exploration for potential therapeutic applications in endometrial and other tissue injuries.

Graphical Abstract

子宫内膜损伤后的修复过程涉及免疫细胞和干细胞等多种细胞的协同作用。本研究将纤溶酶原（Fg）与聚（L-乳酸）-共聚（ε-己内酯）（P(LLA-CL)）通过电纺结合后，将Fg/P(LLA-CL)置入子宫内膜损伤大鼠的宫腔，生物信息学分析表明Fg/P(LLA-CL)可能影响炎症反应和干细胞生物学行为。因此，我们在体外验证了Fg/P(LLA-CL)能抑制脂多糖（LPS）刺激的巨噬细胞向促炎的M1表型转换。此外，在大鼠子宫内膜损伤模型中，Fg/P(LLA-CL)能有效促进巨噬细胞向抗炎M2表型极化，并增强损伤部位间充质干细胞的存在。总之，Fg/P(LLA-CL)对子宫内膜损伤中巨噬细胞的极化和干细胞行为有显著影响，值得进一步探索其在子宫内膜和其他组织损伤中的潜在治疗应用。

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引用次数: 0