Journal of Materials Science: Materials in Medicine最新文献_第5页

Tauroursodeoxycholic acid combined with selenium accelerates bone regeneration in ovariectomized rats 牛磺脱氧胆酸与硒结合可加速卵巢切除大鼠的骨再生

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-10-15 DOI: 10.1007/s10856-024-06803-0

ZhouShan Tao, Min Yang, Cai-Liang Shen

More recently, increased studies have revealed that antioxidants can cure osteoporosis by inhibiting oxidative stress. Tauroursodeoxycholic acid (TUDCA) and Selenium (Se) have been confirmed to possess potent anti-oxidative effects and accelerate bone regeneration. In addition, very little is currently known about the effects of a combination with Se and TUDCA on bone defects in osteoporotic states. We, therefore, aimed to assess the protective effect of combination with Se and TUDCA on bone regeneration and investigate the effect and underlying mechanisms. When MC3T3-E1 was cultured in the presence of H₂H₂, Se, TUDCA and Se/TUDCA therapy could increase the matrix mineralization and promote expression of anti-oxidative stress markers in MC3T3-E1, while reducing intracellular reactive oxygen species (ROS) and mitochondrial ROS levels. Meanwhile, silent information regulator type 1 (SIRT1) was upregulated in response to Se, TUDCA and Se/TUDCA exposures in H₂H₂ treated-MC3T3-E1. In the OVX rat model, Se, TUDCA and Se/TUDCA showed a clear positive effect against impaired bone repair in osteoporosis. The results above demonstrate that Se/TUDCA exhibits superior efficacy in both cellular and animal experiments, as compared to Se and TUDCA. In conclusion, combination with Se and TUDCA stimulates bone regeneration and is a promising candidate for promoting bone repair in osteoporosis.

Graphical abstract

The release of TUDCA and Se during the degradation of Se/TUDCA can improve the local bone repair ability. At the same time, it can also inhibit cell ROS, and ultimately greatly promote local bone repair.

最近，越来越多的研究发现，抗氧化剂可以通过抑制氧化应激来治疗骨质疏松症。牛磺脱氧胆酸（TUDCA）和硒（Se）已被证实具有强大的抗氧化作用，并能加速骨再生。此外，目前人们对 Se 和 TUDCA 的组合对骨质疏松状态下骨缺损的影响知之甚少。因此，我们的目的是评估 Se 和 TUDCA 联合使用对骨再生的保护作用，并研究其效果和内在机制。在H2H2存在下培养MC3T3-E1时，Se、TUDCA和Se/TUDCA疗法可增加MC3T3-E1的基质矿化，促进抗氧化应激标志物的表达，同时降低细胞内活性氧（ROS）和线粒体ROS水平。同时，在H2H2处理的MC3T3-E1中，沉默信息调节因子1型（SIRT1）在Se、TUDCA和Se/TUDCA暴露下上调。在 OVX 大鼠模型中，Se、TUDCA 和 Se/TUDCA 对骨质疏松症中受损的骨修复有明显的积极作用。上述结果表明，与 Se 和 TUDCA 相比，Se/TUDCA 在细胞和动物实验中都表现出更优越的疗效。图解摘要 Se/TUDCA 降解过程中释放的 TUDCA 和 Se 能提高局部骨修复能力。同时，它还能抑制细胞的 ROS，最终极大地促进局部骨修复。

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引用次数: 0

A particle-filled hydrogel based on alginate and calcium phosphate nanoparticles as bone adhesive 基于海藻酸盐和磷酸钙纳米颗粒的颗粒填充水凝胶作为骨粘合剂

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-10-14 DOI: 10.1007/s10856-024-06798-8

Benedikt Kruse, Katarina Vasic, Kai O. Böker, Arndt F. Schilling, Wolfgang Lehmann, Matthias Epple

The clinical need for bone adhesives as an alternative to osteosynthesis is evident. However, this is a challenging problem due to the moist environment in surgical sites with bone surfaces covered with blood and biomolecules like lipids or proteins. A nanoparticle-loaded hydrogel that is based on a freeze-dried powder of silica-coated calcium phosphate/carboxymethyl cellulose nanoparticles (CaP/CMC/SiO₂) and an aqueous solution of sodium alginate (2 wt%) was developed and optimized with respect to the gluing ability in air and in water. The final paste was crosslinked within about one minute by calcium ions released from the calcium phosphate nanoparticles and contained about 20 wt% nanoparticles and 80 wt% water. The mechanical properties of the hydrogel were determined by extensive rheological tests. The thixotropic pasty hydrogel can be applied with a syringe. The adhesion strength was about 84 kPa between moist bone fragments in air. The hydrogel kept fragments of cortical bone well connected for >3 months during complete submersion in water. Besides water, the material consists only of biocompatible and biodegradable components (calcium phosphate, CMC, alginate). It carries only a very low dose of these materials into the bone site (mainly calcium phosphate nanoparticles). In-vitro cell culture with hMSCs that differentiated to osteoblasts confirmed a good biocompatibility of the bone adhesive formulation.

Graphical Abstract

临床上显然需要骨粘合剂来替代骨合成。然而，由于手术部位环境潮湿，骨表面覆盖着血液和脂质或蛋白质等生物大分子，因此这是一个具有挑战性的问题。我们开发了一种纳米颗粒载荷水凝胶，这种水凝胶基于硅包覆的磷酸钙/羧甲基纤维素纳米颗粒（CaP/CMC/SiO2）冻干粉和海藻酸钠（2 wt%）水溶液，并对其在空气中和水中的粘合能力进行了优化。最终的糊状物在约一分钟内由磷酸钙纳米粒子释放的钙离子交联，其中含有约 20 wt% 的纳米粒子和 80 wt% 的水。水凝胶的机械性能是通过大量流变测试确定的。这种触变性糊状水凝胶可以用注射器注射。潮湿的骨碎片在空气中的粘附强度约为 84 kPa。在完全浸入水中的 3 个月内，水凝胶都能使皮质骨碎片保持良好的连接。除了水之外，这种材料只包含生物相容性和可生物降解的成分（磷酸钙、CMC、海藻酸）。它只将极低剂量的这些材料（主要是磷酸钙纳米颗粒）带入骨骼部位。用分化成成骨细胞的高密度骨髓造血干细胞进行体外细胞培养，证实了骨粘合剂配方具有良好的生物相容性。

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引用次数: 0

Bone augmentation using bioresorbable mesh domes containing bone graft granules 使用含有骨移植颗粒的生物可吸收网状穹顶进行骨增量。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-10-07 DOI: 10.1007/s10856-024-06833-8

Toshiki Yanagisawa, Koichiro Hayashi, Kunio Ishikawa

Bone graft granules are valuable tools for ridge area bone grafting owing to their ease of manipulation and interconnected porous structure. Guided bone regeneration (GBR) using barrier membranes is commonly used for alveolar ridge augmentation; however, the surgical procedures are technically complicated. In this study, we fabricated bioresorbable mesh domes (BMDs) using two types of Vicryl mesh (woven and knitted types) containing carbonate apatite granules. BMD samples were prepared in three groups: upper sides made from the woven type (UW) and lower sides made from the woven type (LW) (the UW/LW group), upper sides made from the woven type (UW) and lower sides made from the knitted type (LK) (the UW/LK group), and upper sides made from the knitted type (UK) and lower sides made from the knitted type (LK) (the UK/LK group). The samples were subsequently implanted into rabbit calvaria, and radiomorphometric and histological analyses were conducted. The UK/LK group exhibited enhanced appositional bone formation because the knitted mesh on the skin side prevented the infiltration of a substantial amount of fibrous tissue. This increase in bone formation could be attributed to the interaction between granules and osteoprogenitors that pass through the mesh from the host bone. Conversely, the UW/LW and UW/LK groups presented limited appositional bone formation. Compared with knitted mesh, woven mesh might tend to be absorbed over a short span, allowing fibrous tissue invasion and inhibiting new bone formation. Additionally, BMDs could retain granules in a targeted location and avoid displacement of the granules to unintended locations.

Graphical Abstract

骨移植颗粒因其易于操作和相互连接的多孔结构而成为牙脊区骨移植的重要工具。使用屏障膜进行引导骨再生（GBR）通常用于牙槽嵴增量，但手术过程技术复杂。在这项研究中，我们使用两种含有碳酸盐磷灰石颗粒的 Vicryl 网（编织型和针织型）制作了生物可吸收网状穹顶（BMD）。制备的 BMD 样品分为三组：上侧由编织型（UW）制成，下侧由编织型（LW）制成（UW/LW 组）；上侧由编织型（UW）制成，下侧由针织型（LK）制成（UW/LK 组）；上侧由针织型（UK）制成，下侧由针织型（LK）制成（UK/LK 组）。随后将样品植入兔子的小腿，并进行放射形态计量学和组织学分析。UK/LK组显示出更强的附着骨形成能力，这是因为皮肤一侧的编织网阻止了大量纤维组织的渗入。骨形成的增加可归因于颗粒与从宿主骨穿过网眼的造骨细胞之间的相互作用。相反，UW/LW 组和 UW/LK 组的附着骨形成有限。与针织网相比，编织网可能倾向于在短时间内被吸收，从而允许纤维组织侵入并抑制新骨形成。此外，BMD 可以将颗粒保留在目标位置，避免颗粒移位到非预期位置。

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引用次数: 0

A modular approach to 3D-printed bilayer composite scaffolds for osteochondral tissue engineering 用于骨软骨组织工程的三维打印双层复合材料支架的模块化方法。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-10-07 DOI: 10.1007/s10856-024-06824-9

Maryam Maherani, Hossein Eslami, Seyed Ali Poursamar, Mojtaba Ansari

Prolonged osteochondral tissue engineering damage can result in osteoarthritis and decreased quality of life. Multiphasic scaffolds, where different layers model different microenvironments, are a promising treatment approach, yet stable joining between layers during fabrication remains challenging. To overcome this problem, in this study, a bilayer scaffold for osteochondral tissue regeneration was fabricated using 3D printing technology which containing a layer of PCL/hydroxyapatite (HA) nanoparticles and another layer of PCL/gelatin with various concentrations of fibrin (10, 20 and 30 wt.%). These printed scaffolds were evaluated with SEM (Scanning Electron Microscopy), FTIR (Fourier Transform Infrared Spectroscopy) and mechanical properties. The results showed that the porous scaffolds fabricated with pore size of 210–255 µm. Following, the ductility increased with the further addition of fibrin in bilayer composites which showed these composites scaffolds are suitable for the cartilage part of osteochondral. Also, the contact angle results demonstrated the incorporation of fibrin in bilayer scaffolds based on PCL matrix, can lead to a decrease in contact angle and result in the improvement of hydrophilicity that confirmed by increasing the degradation rate of scaffolds containing further fibrin percentage. The bioactivity study of bilayer scaffolds indicated that both fibrin and hydroxyapatite can significantly improve the cell attachment on fabricated scaffolds. The MTT assay, DAPI and Alizarin red tests of bilayer composite scaffolds showed that samples containing 30% fibrin have the more biocompatibility than that of samples with 10 and 20% fibrin which indicated the potential of this bilayer scaffold for osteochondral tissue regeneration.

Graphical Abstract

长期的骨软骨组织工程损伤会导致骨关节炎和生活质量下降。多层支架可模拟不同的微环境，是一种很有前景的治疗方法，但在制造过程中各层之间的稳定连接仍具有挑战性。为了克服这一问题，本研究利用三维打印技术制作了一种用于骨软骨组织再生的双层支架，其中包含一层 PCL/羟基磷灰石（HA）纳米颗粒和另一层含有不同浓度纤维蛋白（10、20 和 30 wt.%）的 PCL/明胶。用扫描电子显微镜（SEM）、傅立叶变换红外光谱（FTIR）和机械性能对这些印刷支架进行了评估。结果表明，多孔支架的孔径为 210-255 微米。双层复合材料中进一步添加纤维蛋白后，延展性增加，这表明这些复合材料支架适用于骨软骨的软骨部分。此外，接触角结果表明，在基于 PCL 基质的双层支架中加入纤维蛋白，可导致接触角减小，从而改善亲水性，这一点可通过增加含纤维蛋白比例的支架的降解率得到证实。双层支架的生物活性研究表明，纤维蛋白和羟基磷灰石都能显著改善细胞在所制支架上的附着。对双层复合支架进行的 MTT 检测、DAPI 和茜素红检测表明，含 30% 纤维蛋白的样品比含 10% 和 20% 纤维蛋白的样品具有更好的生物相容性，这表明这种双层支架具有骨软骨组织再生的潜力。

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引用次数: 0

Cartilage defect repair in a rat model via a nanocomposite hydrogel loaded with melatonin-loaded gelatin nanofibers and menstrual blood stem cells: an in vitro and in vivo study 大鼠模型软骨缺损的修复：载入褪黑素明胶纳米纤维和月经血干细胞的纳米复合水凝胶的体外和体内研究。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-09-30 DOI: 10.1007/s10856-024-06820-z

Libo Yuan, Ling Yao, Xianzhen Ren, Xusheng Chen, Xu Li, Yongqing Xu, Tao Jin

Cartilage damage caused by injuries or degenerative diseases remains a major challenge in the field of regenerative medicine. In this study, we developed a composite hydrogel system for the delivery of melatonin and menstrual blood stem cells (MenSCs) to treat a rat model of cartilage defect. The composite delivery system was produced by incorporation of melatonin into the gelatin fibers and dispersing these fibers into calcium alginate hydrogels. Various characterization methods including cell viability assay, microstructure studies, degradation rate measurement, drug release, anti-inflammatory assay, and radical scavenging assay were used to characterize the hydrogel system. MenSCs were encapsulated within the nanocomposite hydrogel and implanted into a rat model of full-thickness cartilage defect. A 1.3 mm diameter drilled in the femoral trochlea and used for the in vivo study. Results showed that the healing potential of nanocomposite hydrogels containing melatonin and MenSCs was significantly higher than polymer-only hydrogels. Our study introduces a novel composite hydrogel system, combining melatonin and MenSCs, demonstrating enhanced cartilage repair efficacy, offering a promising avenue for regenerative medicine.

Graphical Abstract

受伤或退行性疾病导致的软骨损伤仍是再生医学领域的一大挑战。在这项研究中，我们开发了一种复合水凝胶系统，用于输送褪黑素和月经血干细胞（MenSCs），以治疗大鼠软骨缺损模型。这种复合输送系统是通过将褪黑素加入明胶纤维并将这些纤维分散到海藻酸钙水凝胶中制成的。水凝胶系统的表征采用了多种表征方法，包括细胞活力测定、微观结构研究、降解率测量、药物释放、抗炎测定和自由基清除测定。将门静脉干细胞包裹在纳米复合水凝胶中，然后植入大鼠全厚软骨缺损模型。在股骨蹄骨上钻孔，直径为 1.3 毫米，用于体内研究。结果表明，含有褪黑激素和造血干细胞的纳米复合水凝胶的愈合潜力明显高于纯聚合物水凝胶。我们的研究介绍了一种新型复合水凝胶系统，它结合了褪黑激素和造血干细胞，显示出更强的软骨修复功效，为再生医学提供了一条前景广阔的途径。

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引用次数: 0

Inhibitory impact of a mesoporous silica nanoparticle-based drug delivery system on Porphyromonas gingivalis-induced bone resorption 基于介孔二氧化硅纳米颗粒的给药系统对牙龈卟啉单胞菌诱导的骨吸收的抑制作用

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-09-30 DOI: 10.1007/s10856-024-06827-6

Mengya Li, Jian Sun, Dong Zhao, Wen Zhang, Qingan Xu

Controlling and reducing plaque formation plays a pivotal role in preventing and treating periodontal disease, often utilizing antibacterial drugs to enhance therapeutic outcomes. Mesoporous silica nanoparticles (MSN), an FDA-approved inorganic nanomaterial, possess robust physical and chemical properties, such as adjustable pore size and pore capacity, easy surface modification, and high biosafety. Numerous studies have exploited MSN to regulate drug release and facilitate targeted delivery. This study aimed to synthesize an MSN-tetracycline (MSN-TC) complex and investigate its inhibitory potential on Porphyromonas gingivalis (P. gingivalis)-induced bone resorption. The antibacterial efficacy of MSN-TC was evaluated through bacterial culture experiments. A P. gingivalis-induced bone resorption model was constructed by subcutaneously injecting P. gingivalis around the cranial bone of rats. Micro-computed tomography was employed to assess the inhibitory impact of MSN and MSN-TC on bone resorption. Furthermore, the influence of MSN and MSN-TC on osteoclast differentiation was examined in vitro. The MSN exhibited optimal pore size and particle dimensions for effective loading and gradual release of TC. MSN-TC demonstrated significant bacteriostatic activity against P. gingivalis. MSN-TC-treated rats showed significantly reduced cranial bone tissue destruction compared to MSN or TC-treated rats. Additionally, both MSN and MSN-TC exhibited inhibitory effects on the receptor activator of nuclear factor kappa-Β ligand-mediated osteoclast differentiation. The MSN-TC complex synthesized in this study demonstrated dual efficacy by exerting antibacterial effects on P. gingivalis and by resisting osteoclast differentiation, thereby mitigating bone resorption induced by P. gingivalis.

Graphical Abstract

控制和减少牙菌斑的形成在预防和治疗牙周病方面起着至关重要的作用，通常利用抗菌药物来提高治疗效果。介孔二氧化硅纳米粒子（MSN）是一种经美国食品及药物管理局批准的无机纳米材料，具有强大的物理和化学特性，如孔径和孔容量可调、表面易于改性以及生物安全性高。许多研究利用 MSN 来调节药物释放和促进靶向给药。本研究旨在合成一种 MSN-四环素（MSN-TC）复合物，并研究其对牙龈卟啉菌（P. gingivalis）诱导的骨吸收的抑制潜力。通过细菌培养实验评估了 MSN-TC 的抗菌功效。通过在大鼠颅骨周围皮下注射牙龈弧菌，建立了牙龈弧菌诱导的骨吸收模型。采用显微计算机断层扫描评估了 MSN 和 MSN-TC 对骨吸收的抑制作用。此外，还在体外研究了 MSN 和 MSN-TC 对破骨细胞分化的影响。MSN 表现出最佳的孔径和颗粒尺寸，可有效装载并逐渐释放 TC。MSN-TC 对牙龈脓疱病具有显著的抑菌活性。与 MSN 或 TC 处理的大鼠相比，MSN-TC 处理的大鼠的颅骨组织破坏明显减少。此外，MSN 和 MSN-TC 对核因子卡巴Β配体受体激活剂介导的破骨细胞分化均有抑制作用。本研究中合成的 MSN-TC 复合物具有双重功效，既能对牙龈脓疱疮产生抗菌作用，又能抑制破骨细胞分化，从而减轻牙龈脓疱疮引起的骨吸收。

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引用次数: 0

Selective mycobacterial capture with ultraviolet-polymerized poly-dimethyldiallyl chloride functionalized surfaces 利用紫外线聚合的聚二甲基二烯丙基氯功能化表面选择性捕获分枝杆菌。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-09-30 DOI: 10.1007/s10856-024-06829-4

Xuesong Jiang, Bonolo S. P. Mathekga, Digvijay Singh, Devin Coon, Anjana Sinha, Derek Armstrong, Soumyadipta Acharya, Hai-Quan Mao, Yukari C. Manabe

Tuberculosis (TB) is the top cause of death from a single infectious pathogen after COVID-19. Despite molecular diagnostic advances, two-thirds of the 10 million annual TB cases are still diagnosed using direct smear microscopy which has ~50% sensitivity. To increase the analytical performance of smear microscopy, we developed and characterized a novel polymer (Polydiallyldimethylammonium chloride [PDADMAC]) engraftment on inexpensive polystyrene (PS) specifically functionalized for mycobacterial capture. Engraftment is achieved via UV photopolymerization of DADMAC monomer on plasma-activated PS. The platform was tested on sputum from presumptive TB cases in Kampala, Uganda (n = 50), with an increased overall sensitivity of 81.8% (27/33) vs. fluorescent smear microscopy 57% (19/33) compared to a molecular (Cepheid GeneXpert MTB/RIF) gold standard. Frugal smear diagnostic innovation that is rapid and does not require dedicated instrumentation may offer an important solution to bridge the TB diagnostic gap.

Graphical Abstract

结核病（TB）是仅次于 COVID-19 的单一传染性病原体致死率最高的病因。尽管分子诊断技术不断进步，但每年 1000 万结核病例中仍有三分之二是通过灵敏度约为 50% 的直接涂片显微镜诊断出来的。为了提高涂片显微镜的分析性能，我们开发了一种新型聚合物（聚二烯丙基二甲基氯化铵 [PDADMAC]），并对其进行了表征。接枝是通过 DADMAC 单体在等离子活化 PS 上的紫外光聚合作用实现的。该平台在乌干达坎帕拉的推定肺结核病例（n = 50）的痰液中进行了测试，与分子（Cepheid GeneXpert MTB/RIF）黄金标准相比，总体灵敏度提高了 81.8%（27/33），而荧光涂片显微镜检查灵敏度为 57%（19/33）。快速、无需专用仪器的低成本涂片诊断创新可为弥合结核病诊断差距提供重要的解决方案。

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引用次数: 0

3D printed polycaprolactone/gelatin/ordered mesoporous calcium magnesium silicate nanocomposite scaffold for bone tissue regeneration 用于骨组织再生的三维打印聚己内酯/明胶/有序介孔硅酸钙镁纳米复合材料支架。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-09-30 DOI: 10.1007/s10856-024-06828-5

Zahra Mirzavandi, Seyed Ali Poursamar, Farshad Amiri, Ashkan Bigham, Mohammad Rafienia

Tissue engineering scaffolds are three-dimensional structures that provide an appropriate environment for cellular attachment, proliferation, and differentiation. Depending on their specific purpose, these scaffolds must possess distinct features, including appropriate mechanical properties, porosity, desired degradation rate, and cell compatibility. This investigation aimed to fabricate a new nanocomposite scaffold using a 3D printing technique composed of poly(ε-caprolactone) (PCL)/Gelatin (GEL)/ordered mesoporous calcium-magnesium silicate (om-CMS) particles. Different weight ratios of om-CMS were added and optimized, and a series of scaffolds were constructed for comparison purposes, including PCL 50%/Gel 50%, PCL 50%/Gel 45%/om-CMS%5, and PCL 50%/Gel 40%/om-CMS%10. The optimized weight ratio of om-CMS was 10% without leaving behind negative effects on the filaments’ structure. The scaffolds’ physical and chemical properties were assessed using various techniques, and their degradation rate, bioactivity potential, cell viability, attachment, and ALP activity were evaluated in vitro. The results demonstrated that the PCL 50%/Gel 40%/om-CMS10% scaffold had promising potential for further studies in bone tissue regeneration.

Graphical Abstract

组织工程支架是一种三维结构，可为细胞附着、增殖和分化提供适当的环境。根据其特定用途，这些支架必须具备不同的特征，包括适当的机械性能、孔隙率、所需的降解率和细胞兼容性。本研究旨在利用三维打印技术制造一种新型纳米复合材料支架，由聚ε-己内酯（PCL）/明胶（GEL）/有序介孔硅酸钙镁（om-CMS）颗粒组成。研究人员添加并优化了不同重量比的om-CMS，并构建了一系列支架进行比较，包括PCL 50%/凝胶50%、PCL 50%/凝胶45%/om-CMS%5和PCL 50%/凝胶40%/om-CMS%10。优化的 om-CMS 重量比为 10%，不会对丝状物的结构产生负面影响。使用各种技术评估了支架的物理和化学特性，并在体外评估了其降解率、生物活性潜能、细胞活力、附着力和 ALP 活性。结果表明，PCL 50%/凝胶 40%/om-CMS10%支架在骨组织再生方面具有进一步研究的潜力。

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引用次数: 0

Sustained co-release of ciprofloxacin and dexamethasone in rabbit maxillary sinus using polyvinyl alcohol-based hydrogel microparticle 利用聚乙烯醇基水凝胶微粒在兔上颌窦中持续释放环丙沙星和地塞米松

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-09-30 DOI: 10.1007/s10856-024-06832-9

Maryam Jalessi, Yasaman Tavakoli Moghaddam, Mehdi Khanmohammadi, Sajad Hassanzadeh, Zahra Azad, Mohammad Farhadi

Topical delivery to paranasal sinuses through sustained-release stents is one of the new horizons in treating chronic rhinosinusitis (CRS). This study aims to introduce and evaluate sustained co-release of encapsulated ciprofloxacin (CIP) and dexamethasone (DEX) in polyvinyl alcohol-based carriers within the maxillary sinus of rabbit animals. DEX and CIP were loaded in a tyramine-substituted polyvinyl alcohol microparticle (PVATyr MP). The mechanical stability, degradability, and sustained-release patterns of both drugs as well as cellular cytocompatibility were assessed in vitro. The PVATyr MPs were then injected into the maxillary sinus of rabbits and they were monitored weekly for 21 days. Nasal endoscopy, MRI imaging, and tissue microscopy were used to follow the changes and compared them with the control condition. Also, the concentrations of drugs were evaluated in the maxillary sinus and blood samples over the study period. Produced PVA-based MPs possessed a relatively narrow particle size distribution (CV 7.7%) with proper physical stability until 30 days of incubation. The uniform-sized PVATyr MPs and their surrounding hydrogel showed sustained-release profiles for DEX and CIP for up to 32 days in vitro. The injected drugs-loaded hydrogel showed complete clearance from the maxillary sinus of rabbits within 28 days. The concentrations of DEX and CIP in mucosal remained within the therapeutic window when measured on days 7, 14, and 21, which were well above the plasma concentrations without any pathological changes in endoscopy, MRI imaging, and histological examinations. DEX/CIP loaded PVATyr MPs provided an effective, controlled, and safe sustained-drug delivery in both in vitro and in vivo analyses at therapeutic concentrations with minimal systemic absorption, suggesting a promising treatment approach for CRS.

Graphical Abstract

通过缓释支架向副鼻窦局部给药是治疗慢性鼻窦炎（CRS）的新领域之一。本研究旨在介绍和评估以聚乙烯醇为载体的环丙沙星（CIP）和地塞米松（DEX）在家兔上颌窦内的持续协同释放。DEX 和 CIP 装载在酪胺取代的聚乙烯醇微颗粒（PVATyr MP）中。在体外评估了这两种药物的机械稳定性、降解性和持续释放模式以及细胞相容性。然后将 PVATyr MPs 注入兔子的上颌窦，每周监测一次，连续 21 天。使用鼻内窥镜、核磁共振成像和组织显微镜来观察其变化，并与对照组进行比较。此外，还对研究期间上颌窦和血液样本中的药物浓度进行了评估。所生产的基于 PVA 的 MPs 具有相对较窄的粒度分布（CV 值为 7.7%），在培养 30 天前具有适当的物理稳定性。尺寸均匀的 PVATyr MPs 及其周围的水凝胶在体外显示了长达 32 天的 DEX 和 CIP 持续释放曲线。注射药物的水凝胶在 28 天内从兔子的上颌窦中完全清除。第 7 天、第 14 天和第 21 天测量的 DEX 和 CIP 在粘膜中的浓度仍在治疗窗口期内，远高于血浆浓度，内窥镜、核磁共振成像和组织学检查均未发现任何病理变化。在体外和体内分析中，负载了 DEX/CIP 的 PVATyr MPs 提供了有效、可控和安全的持续给药，达到了治疗浓度，且全身吸收率极低，是一种治疗 CRS 的有效方法。

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引用次数: 0

On the suitability of photocuring-assisted DIW for manufacturing complex prosthesis from commercial dental composites 用商用牙科复合材料制造复杂义齿的光固化辅助 DIW 的适用性。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-09-30 DOI: 10.1007/s10856-024-06831-w

Florencia M. Nogales, Óscar Borrero-López, Antonia Pajares, Pedro Miranda

A 3-D printing method to produce dental prostheses of complex shapes from a commercial, photocurable resin-ceramic slurry is developed and optimized. The microstructure, mechanical properties and wear behavior of the resulting material are evaluated and compared with a conventional/control sample and other ceramic-polymer dental composites. Commercial resin-ceramic dental slurries can be successfully extruded and appropriately photocured in a low cost 3-D printing system to produce cost-efficient complex dental parts that could be used in indirect restorations. The printing process does not appreciably introduce defects in the material and the 3-D printed composites exhibit mechanical properties (hardness, elastic modulus) and wear resistance comparable to the control material and analogous, conventional dental composites. The main wear mechanisms under sliding contact against a hard antagonist are plastic deformation at the asperity level and ceramic particle pull-out due to filler/matrix interfacial weakness.

Graphical Abstract

3-D printing commercial resin-filler slurries creates cost-efficient tooth prostheses with properties akin to conventional dental composites

本研究开发并优化了一种三维打印方法，可利用商业光固化树脂-陶瓷浆料生产形状复杂的牙科修复体。对所得材料的微观结构、机械性能和磨损行为进行了评估，并与传统/对照样本和其他陶瓷-聚合物牙科复合材料进行了比较。商用树脂-陶瓷牙科泥浆可以在低成本的三维打印系统中成功挤出并适当光固化，从而生产出具有成本效益的复杂牙科部件，可用于间接修复。打印过程不会在材料中产生明显的缺陷，3-D 打印复合材料的机械性能（硬度、弹性模量）和耐磨性与对照材料和类似的传统牙科复合材料相当。在与坚硬的拮抗剂滑动接触时，主要的磨损机制是基底层的塑性变形和由于填料/基质界面薄弱造成的陶瓷颗粒脱落。

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引用次数: 0