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Flow affects the structural and mechanical properties of the fibrin network in plasma clots. 流动会影响血浆凝块中纤维蛋白网络的结构和机械特性。
IF 4.2 3区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-01-29 DOI: 10.1007/s10856-024-06775-1
Hande Eyisoylu, Emma D Hazekamp, Janneke Cruts, Gijsje H Koenderink, Moniek P M de Maat

The fibrin network is one of the main components of thrombi. Altered fibrin network properties are known to influence the development and progression of thrombotic disorders, at least partly through effects on the mechanical stability of fibrin. Most studies investigating the role of fibrin in thrombus properties prepare clots under static conditions, missing the influence of blood flow which is present in vivo. In this study, plasma clots in the presence and absence of flow were prepared inside a Chandler loop. Recitrated plasma from healthy donors were spun at 0 and 30 RPM. The clot structure was characterized using scanning electron microscopy and confocal microscopy and correlated with the stiffness measured by unconfined compression testing. We quantified fibrin fiber density, pore size, and fiber thickness and bulk stiffness at low and high strain values. Clots formed under flow had thinner fibrin fibers, smaller pores, and a denser fibrin network with higher stiffness values compared to clots formed in absence of flow. Our findings indicate that fluid flow is an essential factor to consider when developing physiologically relevant in vitro thrombus models used in researching thrombectomy outcomes or risk of embolization.

纤维蛋白网络是血栓的主要组成部分之一。已知纤维蛋白网络特性的改变会影响血栓疾病的发生和发展,至少部分原因是纤维蛋白的机械稳定性受到了影响。大多数调查纤维蛋白在血栓特性中作用的研究都是在静态条件下制备血栓,而忽略了体内血流的影响。本研究在钱德勒回路中制备了存在和不存在血流的血浆凝块。健康捐献者的再硝化血浆分别以 0 和 30 RPM 的转速旋转。使用扫描电子显微镜和共聚焦显微镜对血凝块结构进行了表征,并将其与无约束压缩试验测得的硬度进行了关联。我们对低应变值和高应变值下的纤维蛋白纤维密度、孔径、纤维厚度和体积刚度进行了量化。与无流动情况下形成的血块相比,流动情况下形成的血块纤维蛋白纤维更细、孔径更小、纤维蛋白网络更致密、刚度值更高。我们的研究结果表明,在开发用于研究血栓切除术效果或栓塞风险的生理学相关体外血栓模型时,流体流动是一个必须考虑的因素。
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引用次数: 0
Doxorubicin-loaded PEG-CdTe QDs conjugated with anti-CXCR4 mAbs: a novel delivery system for extramedullary multiple myeloma treatment 多柔比星负载型 PEG-CdTe QDs 与抗 CXCR4 mAbs 共轭:治疗髓外多发性骨髓瘤的新型递送系统
IF 3.7 3区 医学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-01-20 DOI: 10.1007/s10856-023-06772-w
Dangui Chen, Fei Chen, Jia Lu, Lihong Wang, Fusheng Yao, Haitao Xu

Abstract

Extramedullary multiple myeloma (EMM) is defined as the presence of plasma cells outside the bone marrow of multiple myeloma patients, and its prognosis is poor. High-dose chemotherapy with autologous stem cell transplantation, as a good option on early lines of therapy, has retained the survival benefit of youny EMM patients, but is intolerant for the majority of old patients because of drug cytotoxicity. To essentially address the intolerance above, we designed a CXCR4-PEG-CdTe-DOX (where CXCR4: chemokine receptor 4; PEG-CdTe: polyethylene glycol-modified cadmium telluride; DOX:doxorubicin) nanoplatform. First, CXCR4 is highly expressed in extramedullary plasma cells. Second, PEG-CdTe a drug carrier that controls drug release, can reduce adverse reactions, prolong drug (e.g, DOX) circulation time in the body, and form a targeting carrier after connecting antibodies. In vitro experiments showed CXCR4-PEG-CdTe-DOX facilitated intracellular drug accumulation through active CXCR4 targeting and released DOX into the microenvironment in a pH-controlled manner, enhancing the therapeutic efficacy and apoptosis rate of myeloma cells (U266). Therefore, targeted chemotherapy mediated by CXCR4-PEG-CdTe-DOX is a promising option for EMM treatment.

Graphical abstract

摘要髓外多发性骨髓瘤(EMM)是指多发性骨髓瘤患者骨髓外出现浆细胞,其预后较差。大剂量化疗联合自体干细胞移植作为早期治疗的一个不错选择,保留了髓外多发性骨髓瘤患者的生存优势,但由于药物的细胞毒性,大多数老年患者无法耐受。为了从根本上解决上述不耐受问题,我们设计了一种CXCR4-PEG-CdTe-DOX(其中CXCR4:趋化因子受体4;PEG-CdTe:聚乙二醇修饰的碲化镉;DOX:多柔比星)纳米平台。首先,CXCR4 在髓外浆细胞中高度表达。其次,PEG-CdTe 是一种药物载体,可控制药物释放,减少不良反应,延长药物(如 DOX)在体内的循环时间,并在连接抗体后形成靶向载体。体外实验表明,CXCR4-PEG-CdTe-DOX 通过主动的 CXCR4 靶向作用促进药物在细胞内蓄积,并以 pH 值可控的方式将 DOX 释放到微环境中,提高了疗效和骨髓瘤细胞(U266)的凋亡率。因此,由CXCR4-PEG-CdTe-DOX介导的靶向化疗是EMM治疗的一个很有前景的选择。
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引用次数: 0
Universal Biomaterial-on-Chip: a versatile platform for evaluating cellular responses on diverse biomaterial substrates. 通用生物材料芯片:评估细胞对不同生物材料基底反应的多功能平台。
IF 3.7 3区 医学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-01-11 DOI: 10.1007/s10856-023-06771-x
Abdul Raouf Atif, Morteza Aramesh, Sarah-Sophia Carter, Maria Tenje, Gemma Mestres

Microfluidics has emerged as a promising approach for assessing cellular behavior in vitro, providing more physiologically relevant cell culture environments with dynamic flow and shear stresses. This study introduces the Universal Biomaterial-on-Chip (UBoC) device, which enables the evaluation of cell response on diverse biomaterial substrates in a 3D-printed microfluidic device. The UBoC platform offers mechanical stimulation of the cells and monitoring of their response on diverse biomaterials, enabling qualitative and quantitative in vitro analysis both on- and off-chip. Cell adhesion and proliferation were assessed to evaluate the biocompatibility of materials with different physical properties, while mechanical stimulation was performed to investigate shear-dependent calcium signaling in pre-osteoblasts. Moreover, the applicability of the UBoC platform in creating more complex in vitro models by culturing multiple cell types was demonstrated, establishing a dynamic multicellular environment to investigate cellular interfaces and their significance in biological processes. Overall, the UBoC presents an adaptable tool for in vitro evaluation of cellular behavior, offering opportunities for studying various biomaterials and cell interactions in microfluidic environments.

微流控技术为体外细胞行为评估提供了一种前景广阔的方法,它提供了与生理更相关的具有动态流动和剪切应力的细胞培养环境。本研究介绍了通用片上生物材料(UBoC)装置,它能在三维打印的微流体装置中评估细胞对不同生物材料基底的反应。UBoC 平台可对细胞进行机械刺激,并监测细胞在不同生物材料上的反应,从而在芯片内外进行定性和定量体外分析。对细胞粘附和增殖进行评估是为了评价具有不同物理特性的材料的生物相容性,而机械刺激则是为了研究前成骨细胞的剪切依赖性钙信号传导。此外,还展示了 UBoC 平台通过培养多种细胞类型创建更复杂体外模型的适用性,建立了一个动态多细胞环境来研究细胞界面及其在生物过程中的意义。总之,UBoC 为体外评估细胞行为提供了一种适应性强的工具,为在微流控环境中研究各种生物材料和细胞相互作用提供了机会。
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引用次数: 0
Dexamethasone release from hyaluronic acid microparticle and proanthocyanidin-gelatin hydrogel in sciatic tissue regeneration. 透明质酸微颗粒和原花青素-明胶水凝胶在坐骨神经组织再生中的地塞米松释放。
IF 3.7 3区 医学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-01-11 DOI: 10.1007/s10856-023-06768-6
Kazem Javanmardi, Hamideh Shahbazi, Ava Soltani Hekmat, Mehdi Khanmohammadi, Arash Goodarzi

Biodegradable microparticles are useful vehicles for the controlled release of bioactive molecules in drug delivery, tissue engineering and biopharmaceutical applications. We developed dexamethasone (Dex) encapsulation into tyramine-substituted hyaluronic acid microparticles (Dex-HA-Tyr Mp) mediated by horseradish peroxidase (HRP) crosslinking using a microfluidic device and infollowing crosslinked gelatin (Gela) with proanthocyanidin (PA) as a semi-confined bed hydrogel for the repair of sciatic tissue injury. It was found that the simultaneous use of Dex-HA-Tyr Mp and cross-linked Gela-PA hydrogel improved the physical properties of the hydrogel, including mechanical strength and degradability. The designed composite also provided a sustained release system for Dex delivery to the surrounding sites, demonstrating the applicability of the fabricated hydrogel composite for sciatic nerve tissue engineering and regeneration. The encapsulated cells were viable and showed adequate growth ability and morphogenesis during prolonged incubation in Gela-PA/HA-Tyr Mp hydrogel compared to control conditions. Interestingly, histological analysis revealed a significant increase in the number of axons in the injured sciatic nerve following treatment with Dex-HA-Tyr Mp and injectable Gela-PA hydrogel compared to other control groups. In conclusion, the results demonstrated that fabricated Dex-loaded MPs and injectable hydrogel from biomimetic components are suitable systems for sustained delivery of Dex with adequate biocompatibility and the approach may have potential therapeutic applications in peripheral nerve regeneration.

生物可降解微颗粒是药物递送、组织工程和生物制药应用中控制释放生物活性分子的有用载体。我们利用微流体装置,在辣根过氧化物酶(HRP)交联的介导下,将地塞米松(Dex)包封到酪胺取代的透明质酸微颗粒(Dex-HA-Tyr Mp)中,并在交联明胶(Gela)中加入原花青素(PA)作为半封闭床水凝胶,用于修复坐骨神经组织损伤。研究发现,同时使用 Dex-HA-Tyr Mp 和交联 Gela-PA 水凝胶可改善水凝胶的物理特性,包括机械强度和降解性。所设计的复合材料还提供了一种持续释放系统,可将 Dex 释放到周围部位,证明了所制造的水凝胶复合材料适用于坐骨神经组织工程和再生。与对照组相比,在 Gela-PA/HA-Tyr Mp 水凝胶中长期培养的包裹细胞具有活力,并显示出足够的生长能力和形态发生。有趣的是,组织学分析表明,与其他对照组相比,使用 Dex-HA-Tyr Mp 和注射 Gela-PA 水凝胶处理后,损伤坐骨神经的轴突数量显著增加。总之,研究结果表明,由生物仿生成分制成的Dex负载MPs和可注射水凝胶是持续递送Dex的合适系统,具有足够的生物相容性,这种方法可能在周围神经再生方面有潜在的治疗应用。
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引用次数: 0
Surface characteristics and in vitro biocompatibility of titanium preserved in a vitamin C-containing saline storage solution. 在含维生素 C 的生理盐水保存液中保存的钛的表面特征和体外生物相容性。
IF 3.7 3区 医学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-01-11 DOI: 10.1007/s10856-023-06769-5
Wen-Si Zhang, Yao Liu, Shui-Yi Shao, Chang-Qing Shu, Yi-Heng Zhou, Song-Mei Zhang, Jing Qiu

The purpose of this study is to explore a storage solution for titanium implants and investigate its osteogenic properties. The commercial pure titanium (cp-Ti) surface and double-etched (SLA) titanium surface specimens were preserved in air, saline, 10 mM Vitamin C (VitC)-containing saline and 100 mM VitC-containing saline storage solutions for 2 weeks. The surface microtopography of titanium was observed by scanning electron microscopy (SEM), the surface elemental compositions of the specimens were analyzed by Raman and X-ray photoelectron spectroscopy (XPS), and water contact angle and surface roughness of the specimens were tested. The protein adsorption capacity of two titanium surfaces after storage in different media was examined by BCA kit. The MC3T3-E1 osteoblasts were cultured on two titanium surfaces after storage in different media, and the proliferation, adhesion and osteogenic differentiation activity of osteoblasts were detected by CCK-8, laser confocal microscope (CLSM) and Western blot. The SEM results indicated that the titanium surfaces of the air group were relatively clean while scattered sodium chloride or VitC crystals were seen on the titanium surfaces of the other three groups. There were no significant differences in the micromorphology of the titanium surfaces among the four groups. Raman spectroscopy detected VitC crystals on the titanium surfaces of two experimental groups. The XPS, water contact angle and surface roughness results suggested that cp-Ti and SLA-Ti stored in 0.9% NaCl and two VitC-containing saline storage solutions possessed less carbon contamination and higher surface hydrophilicity. Moreover, the protein adsorption potentials of cp-Ti and SLA-Ti surfaces were significantly improved under preservation in two VitC-containing saline storage solutions. The results of in vitro study showed that the preservation of two titanium surfaces in 100 mM VitC-containing saline storage solution upregulated the cell adhesion, proliferation, osteogenic related protein expressions of MC3T3-E1 osteoblasts. In conclusion, preservation of cp-Ti and SLA-Ti in 100 mM VitC-containing saline storage solution could effectively reduce carbon contamination and enhance surface hydrophilicity, which was conducive to osteogenic differentiation of osteoblasts.

本研究的目的是探索钛植入物的储存方案,并研究其成骨特性。将商用纯钛(cp-Ti)表面和双蚀刻(SLA)钛表面试样分别在空气、生理盐水、10 mM 含维生素 C(VitC)的生理盐水和 100 mM 含维生素 C 的生理盐水中保存 2 周。用扫描电子显微镜(SEM)观察了钛的表面微观形貌,用拉曼光谱和 X 射线光电子能谱(XPS)分析了试样的表面元素组成,并测试了试样的水接触角和表面粗糙度。用 BCA 试剂盒检测了两种钛表面在不同介质中储存后的蛋白质吸附能力。用 CCK-8、激光共聚焦显微镜(CLSM)和 Western blot 检测了两种钛表面在不同培养基中的增殖、粘附和成骨分化活性。扫描电镜结果表明,空气组的钛表面相对干净,而其他三组的钛表面均可见散落的氯化钠或 VitC 晶体。四组钛表面的微观形态没有明显差异。拉曼光谱在两个实验组的钛表面检测到了 VitC 晶体。XPS、水接触角和表面粗糙度结果表明,在 0.9% NaCl 和两种含 VitC 的生理盐水中储存的 cp-Ti 和 SLA-Ti 的碳污染较少,表面亲水性较高。此外,在两种含 VitC 的生理盐水保存液中保存的 cp-Ti 和 SLA-Ti 表面的蛋白质吸附电位明显提高。体外研究结果表明,在 100 mM 含 VitC 的生理盐水保存液中保存两种钛表面可提高 MC3T3-E1 成骨细胞的细胞粘附、增殖和成骨相关蛋白的表达。总之,在 100 mM 含 VitC 的生理盐水保存液中保存 cp-Ti 和 SLA-Ti 可有效减少碳污染,提高表面亲水性,有利于成骨细胞的成骨分化。
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引用次数: 0
Modern methods and materials used to treat root perforation: effectiveness comparison. 治疗牙根穿孔的现代方法和材料:效果比较。
IF 3.7 3区 医学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-01-11 DOI: 10.1007/s10856-023-06770-y
XiaoLan Ma, Hua Xu, Xuefang Chen, Qian Zou, Junrong Wang, Yunmeng Da, Huisu Yin

This study aims to experimentally compare the efficacy of different endodontic materials (iRoot BP Plus, Biodentine, MTA, Rootdent, and Trioxide) in the treatment of pulpitis and perforations on extracted tooth specimens. Additionally, the study aims to investigate the influence of iRoot BP Plus endodontic material on the regenerative processes following pulp amputation in laboratory animals. The secondary goal is to evaluate the effect of iRoot BP Plus on the restoration process in laboratory animals after pulp removal. The study presents a micropermeability analysis of the selected biomaterials performed on a sample of 50 single-rooted apical teeth in 2022. All teeth underwent endodontic treatment. Changes in molar morphology were investigated with eight laboratory animals (rabbits, 3 months old, all males) after simulated pulp removal and subsequent treatment with the iRoot BP Plus biomaterials. iRoot BP Plus appeared to be more effective in retrograde apical root filling than other biomaterials, as evidenced by its higher sealing effect. An experiment involving animal participants revealed the presence of protective adaptive mechanisms, which manifested in the form of an inflammatory process within 6 weeks after the dental pulp was removed. The connective tissue replaced the necrosis, and new capillaries began to form intensively. These dental outcomes suggest that iRoot BP Plus enables hermetical sealing in tooth restoration with good adhesion. Thus, it may have the ability to promote more active tissue regeneration after pulp removal.

本研究旨在通过实验比较不同牙髓材料(iRoot BP Plus、Biodentine、MTA、Rootdent 和 Trioxide)在治疗牙髓炎和拔牙标本穿孔中的疗效。此外,该研究还旨在调查 iRoot BP Plus 根管材料对实验室动物牙髓切断后再生过程的影响。第二个目标是评估 iRoot BP Plus 对实验动物牙髓切除后修复过程的影响。该研究介绍了在 2022 年对 50 颗单根根尖牙齿样本所选生物材料进行的微渗透性分析。所有牙齿均接受过牙髓治疗。研究人员用八只实验动物(兔子,3 个月大,均为雄性)进行了模拟牙髓拔除和随后的 iRoot BP Plus 生物材料治疗后臼齿形态变化的调查。iRoot BP Plus 在逆行根尖充填方面似乎比其他生物材料更有效,其较高的封闭效果就是证明。一项有动物参与的实验显示,牙髓被移除后的 6 周内,存在以炎症过程为表现形式的保护性适应机制。结缔组织取代了坏死组织,新的毛细血管开始密集形成。这些牙科结果表明,iRoot BP Plus 能以良好的粘附性实现牙齿修复的密封。因此,它可能有能力促进牙髓去除后更活跃的组织再生。
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引用次数: 0
Oxali-palladium nanoparticle synthesis, characterization, protein binding, and apoptosis induction in colorectal cancer cells. 氧化钯纳米粒子的合成、特性、蛋白质结合以及对结直肠癌细胞凋亡的诱导作用。
IF 3.7 3区 医学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-01-11 DOI: 10.1007/s10856-023-06766-8
Nasim Golestannezhad, Adeleh Divsalar, Farideh Badalkhani-Khamseh, Milad Rasouli, Arefeh Seyedarabi, Behafarid Ghalandari, Xianting Ding, Fatemeh Goli, Sander Bekeschus, Ali Akbar Moosavi Movahedi, Mahboube Eslami Moghadam

This paper focuses on the synthesis of nano-oxali-palladium coated with turmeric extract (PdNPs) using a green chemistry technique based on the reduction in the Pd (II) complex by phytochemicals inherent in turmeric extract. PdNPs were examined and characterized using Field Emission Scanning Electron Microscopy (FESEM), Dynamic Light Scattering (DLS), Fourier Transform Infrared (FTIR), and Atomic Force Microscopy (AFM). Using different spectroscopic and molecular dynamics simulations, a protein-binding analysis of the produced nanoparticle was conducted by observing its interaction with human serum albumin (HSA). Lastly, the cytotoxic effects and apoptotic processes of PdNPs were studied against the HCT116 human colorectal cell line using the MTT assay and flow cytometry tests. According to the findings, PdNPs with spherical and homogenous morphology and a size smaller than 100 nm were generated. In addition, they can induce apoptosis in colorectal cancer cells in a dose-dependent manner with a lower Cc50 (78 µL) than cisplatin and free oxali-palladium against HCT116 cells. The thermodynamic characteristics of protein binding of nanoparticles with HSA demonstrated that PdNPs had a great capacity for quenching and interacting with HSA through hydrophobic forces. In addition, molecular dynamics simulations revealed that free oxali-palladium and PdNP attach to the same area of HSA via non-covalent interactions. It is conceivable to indicate that the synthesized PdNPs are a potential candidate for the construction of novel, nature-based anticancer treatments with fewer side effects and a high level of eco-friendliness.

本文重点研究了利用姜黄提取物中固有的植物化学物质还原钯(II)络合物的绿色化学技术合成姜黄提取物纳米氧化钯(PdNPs)。使用场发射扫描电子显微镜(FESEM)、动态光散射(DLS)、傅立叶变换红外(FTIR)和原子力显微镜(AFM)对 PdNPs 进行了检测和表征。利用不同的光谱和分子动力学模拟,通过观察生成的纳米粒子与人血清白蛋白(HSA)的相互作用,对其进行了蛋白质结合分析。最后,利用 MTT 试验和流式细胞仪测试研究了 PdNPs 对 HCT116 人结肠直肠细胞系的细胞毒性作用和凋亡过程。研究结果表明,生成的 PdNPs 具有球形和均匀的形态,尺寸小于 100 nm。此外,它们还能以剂量依赖的方式诱导结直肠癌细胞凋亡,对 HCT116 细胞的 Cc50(78 µL)低于顺铂和游离草钯。纳米粒子与 HSA 蛋白结合的热力学特性表明,PdNPs 具有很强的淬灭能力,能通过疏水力与 HSA 相互作用。此外,分子动力学模拟显示,游离草钯和 PdNP 通过非共价作用附着在 HSA 的同一区域。可以想象,合成的 PdNPs 是一种潜在的候选物质,可用于构建新型的、基于自然的抗癌疗法,且副作用小、生态友好性高。
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引用次数: 0
The potential of nano graphene oxide and chlorhexidine composite membranes for use as a surface layer in functionally graded membranes for periodontal lesions 纳米氧化石墨烯和洗必泰复合膜用作牙周病功能分级膜表层的潜力
IF 4.2 3区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2023-12-16 DOI: 10.1007/s10856-023-06767-7
Syed Saad Bin Qasim, Jasim Ahmed, Maribasappa Karched, Adel Al-Asfour

Membranes have been used for treating periodontal defects and play a crucial role in guided bone regeneration applications. Nano graphene oxide have been exploited in tissue engineering due to its biomechanical properties. Its composite formulations with hydroxyapatite and chitosan with controlled degradation could aid in becoming part of a surface layer in a functionally graded membrane. The aim of the study was to synthesize chitosan and composite formulations of nano graphene oxide, hydroxyapatite and chlorhexidine digluconate using solvent casting technique and to characterize the physiochemical, mechanical, water vapor transmission rate (barrier), degradation and antimicrobial potential of the membranes. Altogether four different membranes were prepared (CH, CCG, 3511 and 3322). Results revealed the chemical interactions of hydroxyapatite, chitosan and nanographene oxide due to inter and intra molecular hydrogen bonding. The tensile strength of 3322 (33.72 ± 6.3 MPa) and 3511 (32.06 ± 5.4 MPa) was higher than CH (27.46 ± 9.6 MPa). CCG showed the lowest water vapor transmission rate (0.23 ± 0.01 g/h.m2) but the highest weight loss at day 14 (76.6 %). 3511 showed a higher drug release after 72 h (55.6 %) Significant biofilm growth inhibition was observed for all membranes. 3511 showed complete inhibition against A. actinomycetemcomitans. Detailed characterization of the synthesized membranes revealed that 3511 composite membrane proved to be a promising candidate for use as a surface layer of membranes for guided bone regeneration of periodontal lesions.

Graphical Abstract

薄膜已被用于治疗牙周缺损,并在引导骨再生应用中发挥着重要作用。纳米氧化石墨烯因其生物力学特性已被用于组织工程。其与羟基磷灰石和壳聚糖的复合配方可控制降解,有助于成为功能分级膜表层的一部分。本研究旨在利用溶剂浇铸技术合成壳聚糖以及纳米氧化石墨烯、羟基磷灰石和洗必泰双葡萄糖酸盐的复合配方,并对膜的物理化学、机械、水蒸气透过率(阻隔性)、降解和抗菌潜力进行表征。共制备了四种不同的膜(CH、CCG、3511 和 3322)。结果表明,羟基磷灰石、壳聚糖和纳米氧化石墨因分子间和分子内氢键而产生化学作用。3322 的拉伸强度(33.72 ± 6.3 兆帕)和 3511 的拉伸强度(32.06 ± 5.4 兆帕)高于 CH(27.46 ± 9.6 兆帕)。CCG 的水蒸气透过率最低(0.23 ± 0.01 g/h.m2),但第 14 天的失重率最高(76.6%)。3511 在 72 小时后显示出更高的药物释放率(55.6%)。 所有膜都能显著抑制生物膜的生长。3511 对放线菌有完全的抑制作用。对合成膜进行的详细表征显示,3511 复合膜被证明是一种很有前途的候选材料,可用作牙周病变引导骨再生膜的表层。
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引用次数: 0
A review on the recent applications of synthetic biopolymers in 3D printing for biomedical applications 综述了近年来合成生物聚合物在生物医学3D打印中的应用。
IF 4.2 3区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2023-11-20 DOI: 10.1007/s10856-023-06765-9
Shiva S, Asuwin Prabu R G, Gauri Bajaj, Amy Elsa John, Sharan Chandran, Vishnu Vijay Kumar, Seeram Ramakrishna

3D printing technology is an emerging method that gained extensive attention from researchers worldwide, especially in the health and medical fields. Biopolymers are an emerging class of materials offering excellent properties and flexibility for additive manufacturing. Biopolymers are widely used in biomedical applications in biosensing, immunotherapy, drug delivery, tissue engineering and regeneration, implants, and medical devices. Various biodegradable and non-biodegradable polymeric materials are considered as bio-ink for 3d printing. Here, we offer an extensive literature review on the current applications of synthetic biopolymers in the field of 3D printing. A trend in the publication of biopolymers in the last 10 years are focused on the review by analyzing more than 100 publications. Their application and classification based on biodegradability are discussed. The various studies, along with their practical applications, are elaborated in the subsequent sections for polyethylene, polypropylene, polycaprolactone, polylactide, etc. for biomedical applications. The disadvantages of various biopolymers are discussed, and future perspectives like combating biocompatibility problems using 3D printed biomaterials to build compatible prosthetics are also discussed and the potential application of using resin with the combination of biopolymers to build customized implants, personalized drug delivery systems and organ on a chip technologies are expected to open a new set of chances for the development of healthcare and regenerative medicine in the future.

Graphical Abstract

3D打印技术是一种新兴的技术,受到了世界各国研究者的广泛关注,特别是在卫生和医疗领域。生物聚合物是一种新兴的材料,为增材制造提供了优异的性能和灵活性。生物聚合物广泛应用于生物医学领域,包括生物传感、免疫治疗、药物输送、组织工程和再生、植入物和医疗设备。各种可生物降解和不可生物降解的聚合物材料被认为是3d打印的生物墨水。在这里,我们对合成生物聚合物在3D打印领域的应用进行了广泛的文献综述。通过对100多篇论文的分析,对近10年来生物聚合物的发表趋势进行了综述。讨论了它们的应用和基于生物降解性的分类。在随后的章节中,将详细阐述用于生物医学应用的聚乙烯、聚丙烯、聚己内酯、聚乳酸等的各种研究及其实际应用。讨论了各种生物聚合物的缺点,并讨论了未来的前景,如利用3D打印生物材料来解决生物相容性问题,并讨论了使用树脂与生物聚合物结合来构建定制植入物,个性化药物输送系统和器官芯片技术的潜在应用,预计将为未来的医疗保健和再生医学的发展开辟一系列新的机会。
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引用次数: 0
Electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration 重复双相导电材料电刺激周围神经再生。
IF 4.2 3区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2023-11-15 DOI: 10.1007/s10856-023-06763-x
Tabitha N. Rosenbalm, Nicole H. Levi, Michael J. Morykwas, William D. Wagner

Improved materials for peripheral nerve repair are needed for the advancement of new surgical techniques in fields spanning from oncology to trauma. In this study, we developed bioresorbable materials capable of producing repeated electric field gradients spaced 600 μm apart to assess the impact on neuronal cell growth, and migration. Electrically conductive, biphasic composites comprised of poly (glycerol) sebacate acrylate (PGSA) alone, and doped with poly (pyrrole) (PPy), were prepared to create alternating segments with high and low electrically conductivity. Conductivity measurements demonstrated that 0.05% PPy added to PSA achieved an optimal value of 1.25 × 10−4 S/cm, for subsequent electrical stimulation. Tensile testing and degradation of PPy doped and undoped PGSA determined that 35–40% acrylation of PGSA matched nerve mechanical properties. Both fibroblast and neuronal cells thrived when cultured upon the composite. Biphasic PGSA/PPy sheets seeded with neuronal cells stimulated for with 3 V, 20 Hz demonstrated a 5x cell increase with 1 day of stimulation and up to a 10x cell increase with 3 days stimulation compared to non-stimulated composites. Tubular conduits composed of repeated high and low conductivity materials suitable for implantation in the rat sciatic nerve model for nerve repair were evaluated in vivo and were superior to silicone conduits. These results suggest that biphasic conducting conduits capable of maintaining mechanical properties without inducing compression injuries while generating repeated electric fields are a promising tool for acceleration of peripheral nerve repair to previously untreatable patients.

Graphical Abstract

从肿瘤到创伤,周围神经修复的新手术技术的进步需要改进的材料。在这项研究中,我们开发了生物可吸收材料,能够产生间隔600 μm的重复电场梯度,以评估对神经元细胞生长和迁移的影响。制备了由聚甘油癸二酸酯丙烯酸酯(PGSA)单独组成的导电双相复合材料,并掺杂聚吡咯(PPy),以产生具有高和低导电性的交替段。电导率测量表明,在PSA中添加0.05%的PPy,可获得1.25 × 10-4 S/cm的最佳电导率。PPy掺杂和未掺杂的PGSA的拉伸测试和降解表明,35-40%的PGSA丙烯酸化与神经力学性能相匹配。在复合材料上培养成纤维细胞和神经细胞时,它们都能生长。与未受刺激的复合材料相比,用3 V, 20 Hz刺激神经元细胞的双相PGSA/PPy片在1天的刺激下细胞增加了5倍,在3天的刺激下细胞增加了10倍。通过对高、低电导率重复材料组成的管状导管的体内植入大鼠坐骨神经模型进行神经修复评价,结果表明管状导管优于硅胶管。这些结果表明,在产生重复电场的同时,能够保持机械性能而不引起压迫损伤的双相导电导管是加速周围神经修复以前无法治疗的患者的有前途的工具。
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引用次数: 0
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Journal of Materials Science: Materials in Medicine
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