This study was aimed to present the green synthesis of silver nanoparticles through natural tetraploid Trifolium pratense L. containing high amounts of phenolic compounds and glycosidic bioactive macromolecules. Combinatorial optimization of AgNPs was achieved thanks to the stabilizing and reducing properties of the natural tetraploid T. pratense L. flowering herb extract. The biologically synthesized AgNPs were visualized by color change in UV–Vis spectroscopy. UV–Vis spectroscopy proved to be a fast and simple method to determine the synthesis of AgNPs. In addition, TEM, XRD, and FTIR analyses were performed to confirm and characterize the formation of crystalline silver nanoparticles. It was shown by the TEM analysis that AgNPs changed their size and shape when biosynthesized in different conditions. It was determined that smaller AgNPs were spherical and larger AgNPs had a truncated octahedron shape. The XRD analysis proved the presence of Ag0 in nanoparticles and showed its crystal structure and faced cubic shapes for AgNPs. Interactions between the plant bioactive compounds and the AgNPs were distinctly visible in the FTIR spectra. The biosynthesized AgNPs showed strong antioxidant activity against DPPH and ABTS; furthermore, the total phenol content was also high.
{"title":"Silver nanoparticles production mediated by natural tetraploid Trifolium pratense L.: Characterization and potential biological activity","authors":"Havva Atar Karahan, Hatice Çölgeçen","doi":"10.5586/aa.765","DOIUrl":"https://doi.org/10.5586/aa.765","url":null,"abstract":"\u0000 This study was aimed to present the green synthesis of silver nanoparticles through natural tetraploid Trifolium pratense L. containing high amounts of phenolic compounds and glycosidic bioactive macromolecules. Combinatorial optimization of AgNPs was achieved thanks to the stabilizing and reducing properties of the natural tetraploid T. pratense L. flowering herb extract. The biologically synthesized AgNPs were visualized by color change in UV–Vis spectroscopy. UV–Vis spectroscopy proved to be a fast and simple method to determine the synthesis of AgNPs. In addition, TEM, XRD, and FTIR analyses were performed to confirm and characterize the formation of crystalline silver nanoparticles. It was shown by the TEM analysis that AgNPs changed their size and shape when biosynthesized in different conditions. It was determined that smaller AgNPs were spherical and larger AgNPs had a truncated octahedron shape. The XRD analysis proved the presence of Ag0 in nanoparticles and showed its crystal structure and faced cubic shapes for AgNPs. Interactions between the plant bioactive compounds and the AgNPs were distinctly visible in the FTIR spectra. The biosynthesized AgNPs showed strong antioxidant activity against DPPH and ABTS; furthermore, the total phenol content was also high.","PeriodicalId":6907,"journal":{"name":"Acta Agrobotanica","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43047992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Due to its protein and oil content, soybean is an important crop in developing countries like India to combat energy-protein malnutrition. In a field study, soybean plants- foliar treated with pyraclostrobin- were evaluated for carbon fixation efficiency and antioxidant levels response. Pyraclostrobin (F500) was sprayed over soybean leaves on the tenth and twentieth day after the emergence (DAE) of seedlings at concentrations ranging from 0.05% to 1%. Parameters associated with carbon fixation, like photosystem II efficiency, gas exchange parameters, carbonic anhydrase activity, and biomass accumulation, were quantified on the 45th DAE. Antioxidant level and activity of antioxidant enzymes were also analyzed at the same stage. The amount of antioxidants like ascorbate (ASA) and the activity of antioxidant enzymes (i.e., guaiacol peroxidase, ascorbic acid peroxidase, superoxide dismutase, and catalase) decreased after pyraclostrobin application indicating alleviation of oxidative stress in treated plants as compared to control. A significant increase in the rate of photosynthesis was also recorded in the treated plants. Limiting factors of net photosynthesis like chlorophyll content, carbonic anhydrase activity, stomatal conductance, inter-cellular CO2 concentration, and transpiration rate improved after 0.1% F500 treatment. Mitigation of oxidative stress by pyraclostrobin improves the primary metabolism (fixation) in soybean, resulting in enhanced biomass accumulation.
{"title":"Foliar application of pyraclostrobin alleviates oxidative stress and improves carbon assimilation in soybean","authors":"Juhie Joshi-Paneri, Sonika Sharma, K. Guruprasad","doi":"10.5586/aa.763","DOIUrl":"https://doi.org/10.5586/aa.763","url":null,"abstract":"\u0000 Due to its protein and oil content, soybean is an important crop in developing countries like India to combat energy-protein malnutrition. In a field study, soybean plants- foliar treated with pyraclostrobin- were evaluated for carbon fixation efficiency and antioxidant levels response. Pyraclostrobin (F500) was sprayed over soybean leaves on the tenth and twentieth day after the emergence (DAE) of seedlings at concentrations ranging from 0.05% to 1%. Parameters associated with carbon fixation, like photosystem II efficiency, gas exchange parameters, carbonic anhydrase activity, and biomass accumulation, were quantified on the 45th DAE. Antioxidant level and activity of antioxidant enzymes were also analyzed at the same stage. The amount of antioxidants like ascorbate (ASA) and the activity of antioxidant enzymes (i.e., guaiacol peroxidase, ascorbic acid peroxidase, superoxide dismutase, and catalase) decreased after pyraclostrobin application indicating alleviation of oxidative stress in treated plants as compared to control. A significant increase in the rate of photosynthesis was also recorded in the treated plants. Limiting factors of net photosynthesis like chlorophyll content, carbonic anhydrase activity, stomatal conductance, inter-cellular CO2 concentration, and transpiration rate improved after 0.1% F500 treatment. Mitigation of oxidative stress by pyraclostrobin improves the primary metabolism (fixation) in soybean, resulting in enhanced biomass accumulation.","PeriodicalId":6907,"journal":{"name":"Acta Agrobotanica","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46466765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soil salinity is a severe global stressor causing adverse impacts on irrigated land and drastically reducing crop yields, especially in rice, an important economic crop of Thailand. In this study, the impacts of salt stress on the anatomical and physiological features of 28-day-old rice (Oryza sativa L.) ‘Hom Mali Daeng’ were determined. Various NaCl concentrations (0, 50, 100, 150, and 200 mM) were applied every 2 days, with watering for 2 weeks. The results revealed that salinity stress inhibited the growth of rice. Leaf number, root size, fresh weight, and dry weight were significantly reduced. The electrolyte leakage percentage and malondialdehyde (MDA) content increased after treatment with high NaCl concentrations, while the SPAD unit and chlorophyll content were not significantly different between the control and NaCl treatments. Leaf anatomy changes were studied using freehand section and peeling techniques after salinity stress treatment. Lamina thickness in all treatments decreased, while cell wall and cuticle thickness increased. Stomatal density in all treatments significantly increased. Major vascular bundle, vessel, and phloem area of the 100 mM NaCl treatment were different when compared with the control and other treatments. The results provide information about the physiological and anatomical adaptation of ‘Hom Mali Daeng’ rice, which will be useful for further research in this and other rice cultivars.
土壤盐度是一个严重的全球压力源，对灌溉土地造成不利影响，并大幅降低作物产量，尤其是泰国重要经济作物水稻的产量。本研究测定了盐胁迫对28日龄水稻（Oryza sativa L.）‘Hom Mali Daeng’解剖和生理特性的影响。每2天施用不同浓度的NaCl（0、50、100、150和200mM），并浇水2周。结果表明，盐度胁迫抑制了水稻的生长。叶片数量、根大小、鲜重和干重都显著减少。高浓度NaCl处理后，电解质渗漏率和丙二醛（MDA）含量增加，而对照和NaCl处理的SPAD单位和叶绿素含量没有显著差异。采用徒手切片和去皮技术研究了盐胁迫处理后叶片解剖结构的变化。所有处理的层粘连厚度均降低，而细胞壁和角质层厚度增加。所有处理的气孔密度均显著增加。100mM NaCl处理的主要维管束、血管和韧皮部面积与对照和其他处理相比有所不同。研究结果为“Hom Mali Daeng”水稻的生理和解剖学适应提供了信息，这将有助于该品种和其他水稻品种的进一步研究。
{"title":"Physiological and anatomical response of rice (Oryza sativa L.) ‘Hom Mali Daeng’ at different salinity stress levels","authors":"Worasitikulya Taratima, Chutikarn Samattha, Pitakpong Maneerattanarungroj, Attachai Trunjaruen","doi":"10.5586/aa.764","DOIUrl":"https://doi.org/10.5586/aa.764","url":null,"abstract":"\u0000 Soil salinity is a severe global stressor causing adverse impacts on irrigated land and drastically reducing crop yields, especially in rice, an important economic crop of Thailand. In this study, the impacts of salt stress on the anatomical and physiological features of 28-day-old rice (Oryza sativa L.) ‘Hom Mali Daeng’ were determined. Various NaCl concentrations (0, 50, 100, 150, and 200 mM) were applied every 2 days, with watering for 2 weeks. The results revealed that salinity stress inhibited the growth of rice. Leaf number, root size, fresh weight, and dry weight were significantly reduced. The electrolyte leakage percentage and malondialdehyde (MDA) content increased after treatment with high NaCl concentrations, while the SPAD unit and chlorophyll content were not significantly different between the control and NaCl treatments. Leaf anatomy changes were studied using freehand section and peeling techniques after salinity stress treatment. Lamina thickness in all treatments decreased, while cell wall and cuticle thickness increased. Stomatal density in all treatments significantly increased. Major vascular bundle, vessel, and phloem area of the 100 mM NaCl treatment were different when compared with the control and other treatments. The results provide information about the physiological and anatomical adaptation of ‘Hom Mali Daeng’ rice, which will be useful for further research in this and other rice cultivars.","PeriodicalId":6907,"journal":{"name":"Acta Agrobotanica","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44558425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Tymoszuk, D. Kulus, Angelika Błażejewska, Katarzyna Nadolna, Alicja Kulpińska, Krzysztof Pietrzykowski
Horticulture is now revolutionized by advancements in light-emitting diode (LED) lighting. New technologies enable knowledge expansion on how plants require different spectral illumination for optimal growth and development. The aim of this study was to evaluate the quality of cucumber (Cucumis sativus L. ‘Parys F1’) and tomato (Solanum lycopersicum L. ‘Poranek’) seedlings produced in indoor controlled conditions, using three different types of LED tubes emitting warm light (AP67, AP673L, and G2). The photosynthetic photon flux density was set at 50–65 µmol m−2 s−1 and a 16-hour light regime was used. The results were compared to the cool daylight-emitting fluorescent (FL) control (tube lamp TLD 36W/54). A detailed analysis of the biometrical parameters of the aboveground and underground parts of seedlings was performed. Moreover, the content of chlorophyll in the leaves was measured. No effect of light spectra on the underground part of cucumber was found. On the other hand, the type of lamp affected the number of leaves and chlorophyll content in this species. Lamps AP673L and FL can be recommended in the production of cucumber seedlings. The obtained seedling had characteristics desired for horticultural production, i.e., compact habit with a fair number of leaves and chlorophyll content. As for tomato, the FL lamp had the best effect on the development of seedlings. Due to the high share of far red light, the tested LEDs stimulated the elongated growth of flaccid plants, unsuitable for commercial producers. Our findings not only described the effect of various light spectra on plant development but can also be useful for producers of popular vegetable crops.
{"title":"Application of wide-spectrum light-emitting diodes in the indoor production of cucumber and tomato seedlings","authors":"A. Tymoszuk, D. Kulus, Angelika Błażejewska, Katarzyna Nadolna, Alicja Kulpińska, Krzysztof Pietrzykowski","doi":"10.5586/aa.762","DOIUrl":"https://doi.org/10.5586/aa.762","url":null,"abstract":"\u0000 Horticulture is now revolutionized by advancements in light-emitting diode (LED) lighting. New technologies enable knowledge expansion on how plants require different spectral illumination for optimal growth and development. The aim of this study was to evaluate the quality of cucumber (Cucumis sativus L. ‘Parys F1’) and tomato (Solanum lycopersicum L. ‘Poranek’) seedlings produced in indoor controlled conditions, using three different types of LED tubes emitting warm light (AP67, AP673L, and G2). The photosynthetic photon flux density was set at 50–65 µmol m−2 s−1 and a 16-hour light regime was used. The results were compared to the cool daylight-emitting fluorescent (FL) control (tube lamp TLD 36W/54). A detailed analysis of the biometrical parameters of the aboveground and underground parts of seedlings was performed. Moreover, the content of chlorophyll in the leaves was measured. No effect of light spectra on the underground part of cucumber was found. On the other hand, the type of lamp affected the number of leaves and chlorophyll content in this species. Lamps AP673L and FL can be recommended in the production of cucumber seedlings. The obtained seedling had characteristics desired for horticultural production, i.e., compact habit with a fair number of leaves and chlorophyll content. As for tomato, the FL lamp had the best effect on the development of seedlings. Due to the high share of far red light, the tested LEDs stimulated the elongated growth of flaccid plants, unsuitable for commercial producers. Our findings not only described the effect of various light spectra on plant development but can also be useful for producers of popular vegetable crops.","PeriodicalId":6907,"journal":{"name":"Acta Agrobotanica","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46283234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Milena Tihomirova Nikolova, E. Yankova-Tsvetkova, Tatyana Stefanova, Stoyan Stoyanov, S. Berkov
Compounds accumulated on the surface of plant tissues and structures like glandular trichomes and thin epicuticular layer are defined as exudate, external, superficial. They exhibited important protective activities - antifungal, antibacterial, insect antifeedant, larvicidal, antiplasmodial, and UV protective. The exudate obtained from Helichrysum arenarium flowers was evaluated for its inhibitory activity on germination and initial radicle elongation of Lollium perrene seeds. The experiment was done in vitro in Petri dishes. The exudate, in water–acetone mixture (99.5:0.5), was assayed at concentrations of 1, 3, 5, 7, and 10 mg/mL. The chemical composition of the exudate was analyzed by GC/MS. Exudate solution with 5 mg/mL concentration was found to cause more than 90% of seed germination inhibition. At the same concentration, complete inhibition of root growth was observed. The main bioactive component of exudate was identified as flavonoid aglycone–naringenin. The inhibitory activity of H. arenarium on seed germination was investigated for the first time in the present study.
{"title":"Evaluation of Helichrysum arenarium flower exudate as an inhibitor on Lolium perrene seed germination under laboratory conditions","authors":"Milena Tihomirova Nikolova, E. Yankova-Tsvetkova, Tatyana Stefanova, Stoyan Stoyanov, S. Berkov","doi":"10.5586/aa.761","DOIUrl":"https://doi.org/10.5586/aa.761","url":null,"abstract":"\u0000 Compounds accumulated on the surface of plant tissues and structures like glandular trichomes and thin epicuticular layer are defined as exudate, external, superficial. They exhibited important protective activities - antifungal, antibacterial, insect antifeedant, larvicidal, antiplasmodial, and UV protective. The exudate obtained from Helichrysum arenarium flowers was evaluated for its inhibitory activity on germination and initial radicle elongation of Lollium perrene seeds. The experiment was done in vitro in Petri dishes. The exudate, in water–acetone mixture (99.5:0.5), was assayed at concentrations of 1, 3, 5, 7, and 10 mg/mL. The chemical composition of the exudate was analyzed by GC/MS. Exudate solution with 5 mg/mL concentration was found to cause more than 90% of seed germination inhibition. At the same concentration, complete inhibition of root growth was observed. The main bioactive component of exudate was identified as flavonoid aglycone–naringenin. The inhibitory activity of H. arenarium on seed germination was investigated for the first time in the present study.","PeriodicalId":6907,"journal":{"name":"Acta Agrobotanica","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48824981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sebastian Gadowski, Karolina Tomiczak, Rafał Pietraś, Łukasz Komsta
A set of Gentiana L. species was successfully grown in vitro under the same conditions, and 72 samples from various cultures of these species (root, shoot, cotyledon callus, hypocotyl callus, and root callus) were obtained. The investigated species were G. affinis, G. andrewsii, G. bhutanica, G. burseri, G. cachemirica, G. capitata, G. crassicaulis, G. dahurica, G. decumbens, G. freyniana, G. frigida, G. gelida, G. grossheimii, G. kurroo, G. macrophylla, G. paradoxa, G. robusta, G. scabra, G. septemfida, G. siphonantha, and G. tianschanica. The obtained samples were extracted with a methanol-acetone-water (3:1:1) mixture, evaporated to dryness, and subjected to thin layer chromatography (TLC) on silica gel in sandwich mode with ethyl acetate-methanol-water (8:2:2) as the mobile phase. The resulting dry extracts were subjected to gas chromatography – mass spectrometry (GC-MS) fingerprinting of the headspace volatile fraction. Total ion count and average mass spectrum vectors were collected as two blocks and scaled independently to form a complex dataset. The major direction separating root or shoot samples from callus samples was found not to be fully associated with the highest variance as this information was placed in the first and fourth principal components of the principal component analysis (PCA). Therefore, linear discriminant analysis was performed on the first four (only the informative) components to reveal features responsible for the separation of culture types in the multivariate space.
{"title":"Discriminant Analysis of Gentiana Headspace Gas Chromatography – Mass Spectrometry Extract Data from In Vitro Cultures","authors":"Sebastian Gadowski, Karolina Tomiczak, Rafał Pietraś, Łukasz Komsta","doi":"10.5586/aa.7513","DOIUrl":"https://doi.org/10.5586/aa.7513","url":null,"abstract":"\u0000 A set of Gentiana L. species was successfully grown in vitro under the same conditions, and 72 samples from various cultures of these species (root, shoot, cotyledon callus, hypocotyl callus, and root callus) were obtained. The investigated species were G. affinis, G. andrewsii, G. bhutanica, G. burseri, G. cachemirica, G. capitata, G. crassicaulis, G. dahurica, G. decumbens, G. freyniana, G. frigida, G. gelida, G. grossheimii, G. kurroo, G. macrophylla, G. paradoxa, G. robusta, G. scabra, G. septemfida, G. siphonantha, and G. tianschanica. The obtained samples were extracted with a methanol-acetone-water (3:1:1) mixture, evaporated to dryness, and subjected to thin layer chromatography (TLC) on silica gel in sandwich mode with ethyl acetate-methanol-water (8:2:2) as the mobile phase. The resulting dry extracts were subjected to gas chromatography – mass spectrometry (GC-MS) fingerprinting of the headspace volatile fraction. Total ion count and average mass spectrum vectors were collected as two blocks and scaled independently to form a complex dataset. The major direction separating root or shoot samples from callus samples was found not to be fully associated with the highest variance as this information was placed in the first and fourth principal components of the principal component analysis (PCA). Therefore, linear discriminant analysis was performed on the first four (only the informative) components to reveal features responsible for the separation of culture types in the multivariate space.","PeriodicalId":6907,"journal":{"name":"Acta Agrobotanica","volume":"1 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42492190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Benković-Lačić, B. Japundžić-Palenkić, K. Mirosavljević, Mario Rakić, V. Obradović, Matija Japundžić, R. Benković
In this study, the morphological, pomological, and nutritional values of wild and cultivated rosehip fruits grown in the Slavonia region of eastern Croatia were studied. The results revealed significant differences in several morphological and pomological characteristics among the rosehip genotypes in terms of fruit weight, flesh weight, seed weight, and fruit flesh ratio, with no significant differences in fruit width, fruit length, fruit shape index, seed number per fruit, or seed length. The evaluated rosehip fruit genotypes differed significantly from each other in terms of hectoliter weight (kg), fruit bulk (cm3), and bulk density (kg/m3). For water–soluble extracts, ash, and pH, no statistical difference was found between naturally grown genotypes, but there was a significant difference between naturally grown and cultivated genotypes. Twenty-three major and trace elements were analyzed. The most abundant elements were K, Ca, Mg, and P in both cultivated and naturally grown fruits. The highest concentrations of microelements were Fe, Al, Mn, and Sr. The conventionally cultivated genotype L1 had the highest concentration of Fe and Na as essential elements for humans but also had the highest concentrations of Al, Sr, Ti, V, Cr, Pb, Co, Li, and As of all the genotypes studied. The naturally grown genotype L4 had the highest concentrations of S, Zn, Rb, and Cd and the lowest concentrations of Mg, K, and Ca among all studied genotypes. The data showed that the analyzed genotypes from eastern Croatia had good nutritional quality and variability, making them suitable as genetic resources and possibly leading to the detection of rosehip genotypes as potential sources of beneficial ingredients for human health.
{"title":"Morphological, Pomological, and Nutritional Value of Wild and Cultivated Rosehip (Rosa canina L.) Genotypes in Slavonia, Croatia","authors":"T. Benković-Lačić, B. Japundžić-Palenkić, K. Mirosavljević, Mario Rakić, V. Obradović, Matija Japundžić, R. Benković","doi":"10.5586/aa.7512","DOIUrl":"https://doi.org/10.5586/aa.7512","url":null,"abstract":"In this study, the morphological, pomological, and nutritional values of wild and cultivated rosehip fruits grown in the Slavonia region of eastern Croatia were studied. The results revealed significant differences in several morphological and pomological characteristics among the rosehip genotypes in terms of fruit weight, flesh weight, seed weight, and fruit flesh ratio, with no significant differences in fruit width, fruit length, fruit shape index, seed number per fruit, or seed length. The evaluated rosehip fruit genotypes differed significantly from each other in terms of hectoliter weight (kg), fruit bulk (cm3), and bulk density (kg/m3). For water–soluble extracts, ash, and pH, no statistical difference was found between naturally grown genotypes, but there was a significant difference between naturally grown and cultivated genotypes. Twenty-three major and trace elements were analyzed. The most abundant elements were K, Ca, Mg, and P in both cultivated and naturally grown fruits. The highest concentrations of microelements were Fe, Al, Mn, and Sr. The conventionally cultivated genotype L1 had the highest concentration of Fe and Na as essential elements for humans but also had the highest concentrations of Al, Sr, Ti, V, Cr, Pb, Co, Li, and As of all the genotypes studied. The naturally grown genotype L4 had the highest concentrations of S, Zn, Rb, and Cd and the lowest concentrations of Mg, K, and Ca among all studied genotypes. The data showed that the analyzed genotypes from eastern Croatia had good nutritional quality and variability, making them suitable as genetic resources and possibly leading to the detection of rosehip genotypes as potential sources of beneficial ingredients for human health.","PeriodicalId":6907,"journal":{"name":"Acta Agrobotanica","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44143213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Truong, Nhi Thi Hoang Ho, Phuc Thien Tran, Quang Nhat Ho, An Hoang Nguyen, Thuy Thi Ngoc Lam, L. Nguyen, Nga N T Nguyen
White turmeric (Curcuma aromatica Salisb.) contains notable secondary metabolites with significant health benefits. However, the efficiency of traditional cultivation of white turmeric is limited by many environmental factors. Therefore, it is of great significance to study the large-scale production of superior-quality microrhizomes for cultivation and secondary metabolite extraction, without relying on natural rhizomes. In this study, the effects of sucrose, plant growth regulators (6-benzyl amino purine – BAP, kinetin – KIN,
{"title":"Microrhizome Induction and Curcumin Accumulation in Curcuma aromatica Salisb.","authors":"P. Truong, Nhi Thi Hoang Ho, Phuc Thien Tran, Quang Nhat Ho, An Hoang Nguyen, Thuy Thi Ngoc Lam, L. Nguyen, Nga N T Nguyen","doi":"10.5586/aa.7511","DOIUrl":"https://doi.org/10.5586/aa.7511","url":null,"abstract":"White turmeric (Curcuma aromatica Salisb.) contains notable secondary metabolites with significant health benefits. However, the efficiency of traditional cultivation of white turmeric is limited by many environmental factors. Therefore, it is of great significance to study the large-scale production of superior-quality microrhizomes for cultivation and secondary metabolite extraction, without relying on natural rhizomes. In this study, the effects of sucrose, plant growth regulators (6-benzyl amino purine – BAP, kinetin – KIN,","PeriodicalId":6907,"journal":{"name":"Acta Agrobotanica","volume":"1 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41397080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Gunnula, Nantawan Kanawapee, P. Somta, P. Phansak
Leaf rolling is a common response to drought among members of the grass family. A detailed understanding of the structural changes that occur when rice leaves roll in response to drought may assist in determining the physiological mechanisms underlying this feature and determining its potential utility in plant breeding. The anatomical characteristics of rice cultivars native to northeastern Thailand in response to drought stress were evaluated in this study. Polyethylene glycol (PEG) treatments were applied to seedlings of 28 native rice cultivars and two rice breeding cultivars for 10 d. When cultivated in drought-prone environments, the native rice cultivars had a leaf rolling index between 4 and 7, which was classified as moderate to high leaf rolling. Rice leaf anatomy exhibited profound changes in response to drought, with smaller bulliform cells (36.8%), thicker epidermis (10.98%), smaller vascular bundles (7.41%), and smaller bundle sheath cells (11.95%). Decision trees were used to explore the association between the degree of leaf rolling and anatomical traits. According to the decision tree models generated on the basis of the gain ratio, information gain, and Gini index, the epidermis, leaf thickness, and bulliform cells were the major factors of the root nodes, and the maximum accuracy of the models was 70.90%. Overall, the models indicated that rice leaves with a thin epidermis, large bulliform cells, thin leaves, and small vascular bundles are more likely to display high leaf rolling adaptations under drought stress conditions.
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