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Adipocytokines in Graves' orbitopathy and the effect of high-dose corticosteroids. 巴塞杜氏眼眶病中的脂肪细胞因子和大剂量皮质类固醇的作用。
IF 3.3 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1980258
Jan Schovanek, Michal Krupka, Lubica Cibickova, Marta Karhanova, Sunaina Reddy, Veronika Kucerova, Zdenek Frysak, David Karasek

Graves' orbitopathy (GO) is a serious, progressive eye condition seen in patients with autoimmune thyroid disease. GO is characterized by inflammation and swelling of soft orbital tissues. Adipose tissue produces cytokine mediators called adipokines. The present study focuses on the relationship between serum levels of selected adipokines in patients with GO, comparing them with the control group, and uniquely describes the effect of high-dose systemic corticosteroids (HDSC) on their levels. For the purposes of this study, we collected blood samples before and after the treatment with HDSC from 60 GO patients and 34 control subjects and measured serum levels of adiponectin, AIF-1, A-FABP and FGF-21. Levels of adiponectin significantly differed among the three study groups (ANOVA p = 0.03). AIF-1 levels were also significantly different among the study groups (ANOVA p < 0.0001). AIF-1 was significantly associated with the presence of GO after adjusting for clinical factors (age, sex, smoking and BMI) and level of TSH (odds ratio 1.003, p < 0.01). This finding could enforce targeting macrophages in treatment strategies for GO since AIF-1 is considered as a marker of their activation.

巴塞杜氏眼眶病(Graves' orbitopathy,GO)是一种严重的进行性眼部疾病,多见于自身免疫性甲状腺疾病患者。巴塞杜氏眶病的特点是眼眶软组织发炎和肿胀。脂肪组织会产生被称为脂肪因子的细胞因子介质。本研究的重点是将 GO 患者血清中某些脂肪因子的水平与对照组进行比较,并独特地描述了大剂量全身性皮质类固醇(HDSC)对其水平的影响。在这项研究中,我们采集了 60 名 GO 患者和 34 名对照组患者在接受 HDSC 治疗前后的血样,并测定了血清中的脂肪连素、AIF-1、A-FABP 和 FGF-21 水平。三个研究组的脂肪连素水平存在显著差异(方差分析 p = 0.03)。各研究组的 AIF-1 水平也有明显差异(方差分析 p = 0.03)。
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引用次数: 0
Cleavage of the vaspin N-terminus releases cell-penetrating peptides that affect early stages of adipogenesis and inhibit lipolysis in mature adipocytes. 血管蛋白n端的分裂释放细胞穿透肽,影响脂肪形成的早期阶段并抑制成熟脂肪细胞的脂肪分解。
IF 3.3 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1910154
Catherine A Tindall, Estelle Erkner, Jan Stichel, Annette G Beck-Sickinger, Anne Hoffmann, Juliane Weiner, John T Heiker

Vaspin expression and function is related to metabolic disorders and comorbidities of obesity. In various cellular and animal models of obesity, diabetes and atherosclerosis vaspin has shown beneficial, protective and/or compensatory action. While testing proteases for inhibition by vaspin, we noticed specific cleavage within the vaspin N-terminus and sequence analysis predicted cell-penetrating activity for the released peptides. These findings raised the question whether these proteolytic peptides exhibit biological activity.We synthesized various N-terminal vaspin peptides to investigate cell-penetrating activity and analyse uptake mechanisms. Focusing on adipocytes, we performed microarray analysis and functional assays to elucidate biological activities of the vaspin-derived peptide, which is released by KLK7 cleavage (vaspin residues 21-30; VaspinN). Our study provides first evidence that proteolytic processing of the vaspin N-terminus releases cell-penetrating and bioactive peptides with effects on adipocyte biology. The VaspinN peptide increased preadipocyte proliferation, interfered with clonal expansion during the early stage of adipogenesis and blunted adrenergic cAMP-signalling, downstream lipolysis as well as insulin signalling in mature adipocytes.Protease-mediated release of functional N-terminal peptides presents an additional facet of vaspin action. Future studies will address the mechanisms underlying the biological activities and clarify, if vaspin-derived peptides may have potential as therapeutic agents for the treatment of metabolic diseases.

Vaspin的表达和功能与代谢紊乱和肥胖的合并症有关。在肥胖、糖尿病和动脉粥样硬化的各种细胞和动物模型中,血管蛋白显示出有益、保护和/或代偿作用。在测试vaspin对蛋白酶的抑制作用时,我们注意到vaspin n端有特定的切割,序列分析预测了释放的肽的细胞穿透活性。这些发现提出了这些蛋白水解肽是否具有生物活性的问题。我们合成了多种n端血管肽来研究细胞穿透活性并分析摄取机制。针对脂肪细胞,我们进行了微阵列分析和功能分析,以阐明由KLK7切割释放的vaspin衍生肽的生物活性(vaspin残基21-30;VaspinN)。我们的研究首次提供了血管蛋白n端蛋白水解过程释放细胞穿透和生物活性肽对脂肪细胞生物学影响的证据。VaspinN肽增加了前脂肪细胞的增殖,干扰了脂肪形成早期的克隆扩增,减弱了成熟脂肪细胞的肾上腺素能camp信号、下游脂肪分解和胰岛素信号。蛋白酶介导的功能性n端肽释放是血管素作用的另一个方面。未来的研究将解决潜在的生物活性机制,并澄清血管蛋白衍生肽是否有潜力作为治疗代谢性疾病的治疗剂。
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引用次数: 2
SENP1 is required for the growth, migration, and survival of human adipose-derived stem cells. SENP1是人类脂肪源性干细胞生长、迁移和存活所必需的。
IF 3.3 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2021-12-01 DOI: 10.1080/21623945.2020.1863625
Yingying Wu, Beixin Yu, Min Wang

Human adipose-derived stem cells (hADSCs) are adult mesenchymal cells that have attracted the interest of clinical scientists and surgeons due to their large number of advantages including ease of access and expansion, abundance in cell culture, high proliferative rates, and lower senescence. SUMO/sentrin specific protease 1 (SENP1) is a critical protease that is required during the process of SUMOylation and deSUMOylation, which are dynamic mechanisms that influence cell cycle progression, cell proliferation, and apoptotic status. However, the contribution of SENP1 to these important cellular processes in hADSCs is largely uncharacterized and further studies in this area are required. Here, we show for the first time that after knock out SENP1 in hADSCs, their capacity to migrate and proliferate were inhibited, while apoptosis was enhanced. However, SENP1 did not significantly influence the morphology and MSC-related phenotypes of the hADSCs. These results highlight a role for SENP1 during hADSC growth, and its potential as a therapeutic target to improve the efficacy and safety of hADSCs in the clinic.

人脂肪源性干细胞(hADSCs)是一种成体间充质细胞,由于其易于获取和扩增、细胞培养量丰富、增殖率高、衰老程度低等诸多优点,引起了临床科学家和外科医生的兴趣。SUMO/sentrin特异性蛋白酶1 (SENP1)是SUMOylation和deSUMOylation过程中必需的关键蛋白酶,SUMOylation和deSUMOylation是影响细胞周期进程、细胞增殖和凋亡状态的动态机制。然而,SENP1在hADSCs中对这些重要细胞过程的贡献在很大程度上是未知的,需要在这一领域进行进一步的研究。在这里,我们首次发现敲除hascs中的SENP1后,它们的迁移和增殖能力受到抑制,而细胞凋亡增强。然而,SENP1并没有显著影响hascs的形态和msc相关表型。这些结果强调了SENP1在hADSC生长过程中的作用,以及它作为提高hADSC临床疗效和安全性的治疗靶点的潜力。
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引用次数: 4
A novel immune-related genes signature after bariatric surgery is histologically associated with non-alcoholic fatty liver disease. 减肥手术后一种新的免疫相关基因特征在组织学上与非酒精性脂肪肝相关。
IF 3.3 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1970341
Yancheng Song, Jan Zhang, Hexiang Wang, Dong Guo, Chentong Yuan, Bo Liu, Hao Zhong, Dongmei Li, Yu Li

Increasing evidence shows that immune-related genes (IRGs) play an important role in bariatric surgery (BS). We identified differentially expressed immune-related genes (DEIRGs) of adipose tissue after BS by analysing the two expression profiles of GEO (GSE59034 and GSE29409). Subsequently, enrichment analysis, GSEA and PPI networks were examined to identify the hub IRGs and related pathways. The performance of the signature was evaluated by area under the curve (AUC) of the receiver operating characteristic (ROC). CIBERSORT algorithm was used to evaluate the relative abundance of infiltrated immune cells.42 DEIRGs were found between the GSE59034 and GSE29409 datasets. The AUC of the signature was 0.904 and 0.865 in the GSE58979 and GSE48452, respectively. Interestingly, the signature also showed good performance in diagnosing non-alcoholic fatty liver disease (NAFLD) (AUC was 0.834 and 0.800, respectively). The number of neutrophils, macrophages M2, macrophages M0 and dendritic cells activated decreased significantly. After BS, the infiltration of T cells regulatory, monocytes, mast cells resting and plasma cells in adipose tissue increased. The novel proposed IRGs signature reveals the underlying immune mechanism of BS and is a promising biomarker for distinguishing the severity of NAFLD. This will provide new insights into strategies for treating obesity and NAFLD.

越来越多的证据表明免疫相关基因(IRGs)在减肥手术(BS)中起着重要作用。通过分析GEO的两个表达谱(GSE59034和GSE29409),我们确定了BS后脂肪组织的差异表达免疫相关基因(DEIRGs)。随后,通过富集分析、GSEA和PPI网络来确定中枢IRGs和相关通路。用受试者工作特征(ROC)曲线下面积(AUC)评价信号的有效性。采用CIBERSORT算法评价浸润免疫细胞的相对丰度在GSE59034和GSE29409数据集之间发现DEIRGs。GSE58979和GSE48452的AUC分别为0.904和0.865。有趣的是,该特征在诊断非酒精性脂肪性肝病(NAFLD)方面也表现出良好的性能(AUC分别为0.834和0.800)。活化的中性粒细胞、巨噬细胞M2、巨噬细胞M0和树突状细胞数量明显减少。BS后脂肪组织中调节性T细胞、单核细胞、静止肥大细胞和浆细胞的浸润增加。新提出的IRGs特征揭示了BS的潜在免疫机制,是区分NAFLD严重程度的有希望的生物标志物。这将为肥胖和NAFLD的治疗策略提供新的见解。
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引用次数: 9
Human antigen R promotes angiogenesis of endothelial cells cultured with adipose stem cells derived exosomes via overexpression of vascular endothelial growth factor in vitro. 人抗原R通过体外过表达血管内皮生长因子促进脂肪干细胞来源外泌体培养的内皮细胞的血管生成。
IF 3.3 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1982577
Guo Li, Youbai Chen, Yudi Han, Tian Ma, Yan Han

Recent studies showed that exosomes obtained from adipose-derived stem cells (ADSCs) could improve the angiogenesis of fat grafts via overexpression of vascular endothelial growth factor (VEGF). Human antigen R (HuR) promotes the expression of VEGF in many cancers, but the effect of HuR in normal endothelial cells in the presence of ADSC-derived exosomes remains unclear. We aimed to investigate the effect of HuR on the expression of VEGF and angiogenesis of human umbilical vein endothelial cells (HUVECs) cultured with ADSCs-derived exosomes. The HuR-overexpressed HUVECs (HuR-HUVECs) were cocultured with ADSCs-derived exosomes. qRT-PCR and Western blotting were performed to examine the stability and expression of VEGF-A mRNA and protein. The proliferation, migration, and proangiogenic capacity of HuR-HUVECs were evaluated using cell counting kit-8 (CCK-8), scratch wound healing, and Matrigel tube formation assay. qRT-PCR showed that HuR-HUVECs had higher expression and slower attenuation of VEGF-A mRNA. Western blotting confirmed higher expression of VEGF-A in HuR-HUVECs. CCK-8, scratch wound healing, and Matrigel tube formation assay demonstrated an increased proangiogenic effect in HuR-HUVECs. HuR promotes angiogenesis of HUVECs cocultured with ADSCs-derived exosomes via stabilization and overexpression of VEGF in vitro. The HuR/VEGF pathway is an important regulatory mechanism of angiogenesis in endothelial cells.

最近的研究表明,从脂肪源性干细胞(ADSCs)中获得的外泌体可以通过过度表达血管内皮生长因子(VEGF)来促进脂肪移植物的血管生成。人抗原R (HuR)在许多癌症中促进VEGF的表达,但在adsc衍生外泌体存在的情况下,HuR在正常内皮细胞中的作用尚不清楚。我们的目的是研究HuR对adscs来源的外泌体培养的人脐静脉内皮细胞(HUVECs) VEGF表达和血管生成的影响。将hr过表达的HUVECs (hr -HUVECs)与adscs衍生的外泌体共培养。采用qRT-PCR和Western blotting检测VEGF-A mRNA和蛋白的表达及稳定性。采用细胞计数试剂盒-8 (CCK-8)、划伤愈合和Matrigel管形成实验评估hr - huvecs的增殖、迁移和促血管生成能力。qRT-PCR结果显示,hr - huvecs具有较高的VEGF-A mRNA表达和较慢的衰减。Western blotting证实VEGF-A在hr - huvec中表达较高。CCK-8、划伤愈合和Matrigel管形成试验表明,HuR-HUVECs的促血管生成作用增强。在体外,HuR通过稳定和过表达VEGF促进与adscs来源的外泌体共培养的HUVECs血管生成。HuR/VEGF通路是内皮细胞血管生成的重要调控机制。
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引用次数: 6
Dysfunctional adiposity index as a marker of adipose tissue morpho-functional abnormalities and metabolic disorders in apparently healthy subjects. 功能失调肥胖指数作为表面健康受试者脂肪组织形态功能异常和代谢紊乱的标志。
IF 3.3 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1893452
Juan Reyes-Barrera, Victor H Sainz-Escárrega, Aida X Medina-Urritia, Esteban Jorge-Galarza, Horacio Osorio-Alonso, Margarita Torres-Tamayo, Gabriela Leal-Escobar, Carlos Posadas-Romero, Ivan Torre-Villalvazo, Juan G Juárez-Rojas

Compared to body mass index, waist circumference (WC), and adiposity measurements, adipose tissue (AT) morpho-functionality evaluations are better predictors of cardiometabolic abnormalities (CA). The present study establishes a dysfunctional adiposity index (DAI) as an early marker of CA based on adipocytes morpho-functional abnormalities. DAI was established in 340 subjects without cardiovascular risk factors selected from a cross-sectional study (n=1600). Then, DAI was calculated in 36 healthy subjects who underwent subcutaneous AT biopsy. The correlation of DAI with adipocyte morphology (size/number) and functionality (adiponectin/leptin ratio) was analyzed. The DAI cut-off point was identified and its independent association with CA was determined in 1418 subjects from the cross-sectional study. The constant parameters to calculate the DAI were [WC/[22.79+[2.68*BMI]]]*[triglycerides (TG, mmol/L)/1.37]*[1.19/high density lipoprotein-cholesterol (HDL-C, mmol/L)] for males, and [WC/[24.02+[2.37*BMI]]]*[TG(mmol/L)/1.32]*[1.43/HDL-C(mmol/L)] for females. DAI correlated with adipocytes mean area, adipocyte number and adiponectin/leptin ratio. DAI ≥1.065 was independently associated with diabetes, non-alcoholic fatty liver disease, subclinical atherosclerosis, and hypertension. The present study highlights that DAI is associated with early CA independently of adiposity and other risk factors. Since DAI is obtained using accessible parameters, it can be easily incorporated into clinical practice for early identification of AT abnormalities in apparently healthy subjects.

与体重指数、腰围(WC)和脂肪测量相比,脂肪组织(AT)形态功能评估能更好地预测心脏代谢异常(CA)。本研究基于脂肪细胞形态功能异常建立了功能失调肥胖指数(DAI)作为CA的早期标志物。从横断面研究中选择340名无心血管危险因素的受试者(n=1600)建立DAI。然后,计算36名接受皮下AT活检的健康受试者的DAI。分析DAI与脂肪细胞形态(大小/数量)和功能(脂联素/瘦素比值)的相关性。从横断面研究中确定了1418名受试者的DAI分界点,并确定了其与CA的独立关联。计算DAI的恒定参数为男性[WC/[22.79+[2.68*BMI]]]*[甘油三酯(TG, mmol/L)/1.37]*[1.19/高密度脂蛋白-胆固醇(HDL-C, mmol/L)],女性[WC/[24.02+[2.37*BMI]] *[TG(mmol/L)/1.32]*[1.43/HDL-C(mmol/L)]。DAI与脂肪细胞平均面积、脂肪细胞数量、脂联素/瘦素比值相关。DAI≥1.065与糖尿病、非酒精性脂肪肝、亚临床动脉粥样硬化和高血压独立相关。本研究强调DAI与早期CA相关,独立于肥胖和其他危险因素。由于DAI是使用可获取的参数获得的,因此可以很容易地将其纳入临床实践,用于早期识别表面健康受试者的AT异常。
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引用次数: 17
MiR-33a plays an crucial role in the proliferation of bovine preadipocytes. MiR-33a 在牛前脂肪细胞的增殖过程中起着至关重要的作用。
IF 3.3 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1908655
Wenzhen Zhang, Li Wang, Sayed Haidar Abbas Raza, Xiaoyu Wang, Guohu Wang, Chengcheng Liang, Gong Cheng, Bingzhi Li, Linsen Zan

Preadipocyte proliferation is a critical and precisely orchestrated procedure in adipogenesis, which is highly regulated by microRNAs (miRNAs). A previous study identified that the expression of miR-33a is different in intramuscular fat (IMF) tissues from steers and bulls. In the present study, miR-33a was overexpressed in bovine preadipocytes, and a total of 781 differentialy expressed genes were found, including 348 upregulated and 433 downregulated genes. Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analyses of the differentially expressed genes enriched cell division and cell cycle respectively. MiR-33a overexpression decreased the rate of preadipocyte proliferation. Synchronously, the mRNA and protein expression levels of proliferation-related marker genes, including cyclin B1 (CCNB1) and proliferating cell nuclear antigen (PCNA), were decreased. In contrast, inhibiting miR-33a increased the rate of preadipocyte proliferation, and expression levels of CCNB1 and PCNA. Furthermore, based on luciferase reporter assays, miR-33a targeted directly cyclin-dependent kinase 6 (CDK6)-3'UTR and inhibited CDK6 protein expression. Interestingly, the silencing of CDK6 inhibited bovine preadipocyte proliferation and proliferation-related genes. Therefore, miR-33a inhibits the proliferation of bovine preadipocytes. CDK6 is the target gene of miR-33a and may be involved in the effects of miR-33a on bovine preadipocyte proliferation.

前脂肪细胞增殖是脂肪生成过程中的一个关键和精确协调的过程,它受到微RNA(miRNA)的高度调控。之前的一项研究发现,miR-33a 在母牛和公牛的肌内脂肪(IMF)组织中的表达不同。在本研究中,miR-33a 在牛前脂肪细胞中过表达,共发现 781 个差异表达基因,包括 348 个上调基因和 433 个下调基因。基因本体和京都基因组百科全书(KEGG)对差异表达基因进行了通路分析,发现细胞分裂和细胞周期的差异表达基因较多。MiR-33a的过表达降低了前脂肪细胞的增殖速度。与此同时,细胞周期蛋白 B1(CCNB1)和增殖细胞核抗原(PCNA)等增殖相关标记基因的 mRNA 和蛋白表达水平也下降了。相反,抑制 miR-33a 会增加前脂肪细胞的增殖速度以及 CCNB1 和 PCNA 的表达水平。此外,根据荧光素酶报告实验,miR-33a 直接靶向细胞周期蛋白依赖性激酶 6(CDK6)-3'UTR,抑制了 CDK6 蛋白的表达。有趣的是,CDK6 的沉默抑制了牛前脂肪细胞的增殖和增殖相关基因的表达。因此,miR-33a 能抑制牛前脂肪细胞的增殖。CDK6 是 miR-33a 的靶基因,可能参与了 miR-33a 对牛前脂肪细胞增殖的影响。
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引用次数: 0
Serum amyloid A3 deficiency impairs in vitro and in vivo adipocyte differentiation. 血清淀粉样蛋白A3缺乏损害体外和体内脂肪细胞分化。
IF 3.3 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1916220
Ellen Vercalsteren, Christine Vranckx, Ines Vermeire, Max Gooijen, Roger Lijnen, Ilse Scroyen
ABSTRACT Obesity, caused by an excess adipose tissue, is one of the biggest health-threats of the 21st century. Adipose tissue expansion occurs through two processes: (i) hypertrophy, and (ii) hyperplasia, the formation of new adipocytes, also termed adipogenesis. Recently, serum amyloid A3 (Saa3) has been implicated in adipogenesis. Therefore, the aim of this study was to investigate the effect of Saa3 on adipogenesis using both an in vitro and in vivo murine model. Saa3 gene silenced pre-adipocytes ha a lower expression of pro-adipogenic markers and less lipid accumulation, indicating impaired adipogenesis. Furthermore, male NUDE mice, injected with Saa3 gene silenced pre-adipocytes developed smaller fat pads with smaller adipocytes and lower expression of pro-adipogenic markers than their control counterparts. This confirms that Saa3 gene silencing indeed impairs adipogenesis, both in vitro and in vivo. These results indicate a clear role for Saa3 in adipogenesis and open new perspectives in the battle against obesity.
肥胖是由过多的脂肪组织引起的,是21世纪最大的健康威胁之一。脂肪组织的扩张通过两个过程发生:(i)肥大,(ii)增生,即新脂肪细胞的形成,也称为脂肪生成。最近,血清淀粉样蛋白A3 (Saa3)与脂肪形成有关。因此,本研究的目的是通过体外和体内小鼠模型来研究Saa3对脂肪形成的影响。Saa3基因沉默的前脂肪细胞有较低的促脂肪标志物表达和较少的脂质积累,表明脂肪形成受损。此外,与对照组相比,注射Saa3基因沉默的前脂肪细胞的雄性裸鼠脂肪垫更小,脂肪细胞更小,促脂肪标志物的表达更低。这证实了Saa3基因沉默确实会在体外和体内损害脂肪形成。这些结果表明Saa3在脂肪形成中的明确作用,并为对抗肥胖开辟了新的视角。
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引用次数: 3
Extracellular vesicles derived from lipoaspirate fluid promote fat graft survival. 从吸脂液中提取的细胞外囊泡促进脂肪移植物存活。
IF 3.3 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1932355
Fangfei Nie, Pengbing Ding, Chen Zhang, Zhenmin Zhao, Hongsen Bi

Extracellular vesicles (EVs) are specific subcellular vesicles released by cells under various environmental conditions. Tumescent liposuction is a commonly used procedure in plastic surgery practice. In the present study, we aimed to extract EVs derived from lipoaspirate fluid (LF-EVs) and characterize them using transmission electron microscopy, nanoparticle tracking analysis, and western blotting. The global profiles of proteins and microRNAs from LF-EVs were identified, strongly suggesting a potential regulatory function of LF-EVs. In addition, we investigated the effects and mechanisms of LF-EVs on fat graft survival. Cell functional tests showed that LF-EVs promoted the proliferation, migration, and tube structure formation of human umbilical vein endothelial cells. LF-EVs also promoted the adipogenic differentiation of adipose tissue-derived stem cells. The results of animal experiments showed that the average weights of fat grafts in the LF-EVs-treated group were significantly higher than those in the control group. Histologically, there was less fibrosis, fewer cysts, and increased fat tissue survival in the LF-EVs group. Further investigations of angiogenic and adipogenic factors revealed that LF-EVs also promoted angiogenesis and exerted a pro-adipogenic effect in vivo. Our findings will help to elucidate the functions of LF-EVs and provide a reference dataset for future translational studies.

细胞外囊泡(EVs)是细胞在各种环境条件下释放的特异性亚细胞囊泡。肿胀吸脂术是整形外科实践中常用的一种方法。在本研究中,我们旨在从抽脂液(lf - ev)中提取ev,并使用透射电子显微镜、纳米颗粒跟踪分析和免疫印迹法对其进行表征。研究人员鉴定了lf - ev的蛋白质和microrna的全局谱,强烈表明lf - ev具有潜在的调节功能。此外,我们还研究了lf - ev对脂肪移植存活的影响及其机制。细胞功能实验表明,lf - ev能促进人脐静脉内皮细胞的增殖、迁移和管状结构的形成。lf - ev还能促进脂肪组织源性干细胞的成脂分化。动物实验结果显示,lf - ev处理组脂肪移植体的平均重量明显高于对照组。组织学上,lf - ev组纤维化少,囊肿少,脂肪组织存活率提高。对血管生成因子和脂肪生成因子的进一步研究表明,lf - ev还能促进血管生成,并在体内发挥促脂肪作用。我们的发现将有助于阐明lf - ev的功能,并为未来的转化研究提供参考数据集。
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引用次数: 4
Different effects of high-fat and high-sucrose diets on the physiology of perivascular adipose tissues of the thoracic and abdominal aorta. 高脂高糖日粮对胸腹主动脉血管周围脂肪组织生理机能的不同影响。
IF 3.3 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1965333
Tsukasa Sasoh, Hirona Kugo, Yuya Kondo, Kento Miyamoto, Momoka Minami, Mayo Higashihara, Hirokazu Kawamoto, Fumiaki Takeshita, Tatsuya Moriyama, Nobuhiro Zaima

Vascular diseases such as atherosclerosis and aneurysms are associated with diet. Perivascular adipose tissue (PVAT) was reportedly involved in the regulation of vascular functions. It is suggested that imbalanced diets can cause PVAT inflammation and dysfunction as well as impaired vascular function. However, the association between diets and PVAT are not clearly understood. Here, we showed that a high-fat and a high-sucrose diet affected PVAT at different sites. A high-fat diet induced increased number of large-sized lipid droplets and increased CD (Cluster of differentiation) 68+ macrophage- and monocyte chemotactic protein (MCP)-1-positive areas in the abdominal aortic PVAT (aPVAT). In addition, a high-fat diet caused decreased collagen fibre-positive area and increased CD68+ macrophage- and MCP-1-positive areas in the abdominal aorta. In contrast, a high-sucrose diet induced increased number of large-sized lipid droplets, increased CD68+ macrophage- and MCP-1-positive areas, and decreased UCP-1 positive area in the thoracic aortic PVAT (tPVAT). A high-sucrose diet caused decreased collagen fibre-positive area and increased CD68+ macrophage- and MCP-1-positive areas in the thoracic aorta. These results could be attributed to the different adipocyte populations in the tPVAT and aPVAT. Our results provide pathological evidence to improve our understanding of the relationship between diet and vascular diseases.

动脉粥样硬化和动脉瘤等血管疾病与饮食有关。据报道,血管周围脂肪组织(PVAT)参与了血管功能的调节。提示饮食不平衡可引起PVAT炎症和功能障碍,并导致血管功能受损。然而,饮食与PVAT之间的关系尚不清楚。在这里,我们发现高脂肪和高糖饮食影响不同部位的PVAT。高脂饮食导致腹主动脉PVAT (aPVAT)中大脂滴数量增加,CD (Cluster of differentiation) 68+巨噬细胞和单核细胞趋化蛋白(MCP)-1阳性区域增加。此外,高脂肪饮食导致腹主动脉胶原纤维阳性区域减少,CD68+巨噬细胞和mcp -1阳性区域增加。相反,高糖饮食诱导大鼠胸主动脉PVAT (tPVAT)中大尺寸脂滴数量增加,CD68+巨噬细胞和mcp -1阳性区域增加,UCP-1阳性区域减少。高糖饮食导致胸主动脉胶原纤维阳性区域减少,CD68+巨噬细胞和mcp -1阳性区域增加。这些结果可能归因于tPVAT和aPVAT中不同的脂肪细胞群。我们的结果提供了病理证据,以提高我们对饮食与血管疾病之间关系的理解。
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引用次数: 6
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