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A chromatin accessibility landscape during early adipogenesis of human adipose-derived stem cells 人类脂肪干细胞早期脂肪生成过程中的染色质可及性景观
IF 3.3 4区 生物学 Q2 Medicine Pub Date : 2022-04-17 DOI: 10.1080/21623945.2022.2063015
Sen Li, Xiaolin Zong, Liheng Zhang, Luya Li, Jianxin Wu
ABSTRACT Obesity has become a serious global public health problem; a deeper understanding of systemic change of chromatin accessibility during human adipogenesis contributes to conquering obesity and its related diseases. Here, we applied the ATAC-seq method to depict a high-quality genome‐wide time-resolved accessible chromatin atlas during adipogenesis of human adipose-derived stem cells (hASCs). Our data indicated that the chromatin accessibility drastic dynamically reformed during the adipogenesis of hASCs and 8 h may be the critical transition node of adipogenesis chromatin states from commitment phase to determination phase. Moreover, upon adipogenesis, we also found that the chromatin accessibility of regions related to anti-apoptotic, angiogenic and immunoregulatory gradually increased, which is beneficial to maintaining the health of adipose tissue (AT). Finally, the chromatin accessibility changed significantly in intronic regions of peroxisome proliferator‐activated receptor γ during adipogenesis, and these regions were rich in transcription factors binding motifs that were exposed for further regulation. Overall, we systematically analysed the complex change of chromatin accessibility occurring in the early stage of adipogenesis and deepened our understanding of human adipogenesis. Furthermore, we also provided a good reference data resource of genome‐wide chromatin accessibility for future studies on human adipogenesis.
肥胖已成为严重的全球性公共卫生问题;深入了解人类脂肪形成过程中染色质可及性的系统性变化有助于战胜肥胖及其相关疾病。在这里,我们应用ATAC-seq方法来描绘人类脂肪来源干细胞(hASCs)脂肪形成过程中高质量的全基因组时间分辨率可访问的染色质图谱。我们的数据表明,在hASCs的脂肪形成过程中,染色质可及性发生了剧烈的动态改变,8 h可能是脂肪形成染色质状态从承诺期到决定期的关键过渡节点。此外,在脂肪形成过程中,我们还发现抗凋亡、血管生成和免疫调节相关区域的染色质可及性逐渐增加,这有利于维持脂肪组织(AT)的健康。最后,在脂肪形成过程中,过氧化物酶体增殖物激活受体γ内含子区域的染色质可及性发生了显著变化,这些区域富含转录因子结合基序,可以进一步调控。总的来说,我们系统地分析了脂肪形成早期染色质可及性的复杂变化,加深了我们对人类脂肪形成的认识。此外,我们还为未来人类脂肪形成的全基因组染色质可及性研究提供了良好的参考数据资源。
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引用次数: 3
Dysfunction of insulin-AKT-UCP1 signalling inhibits transdifferentiation of human and mouse white preadipocytes into brown-like adipocytes 胰岛素-AKT-UCP1信号传导的功能障碍抑制人和小鼠白色前脂肪细胞向棕色样脂肪细胞的转分化
IF 3.3 4区 生物学 Q2 Medicine Pub Date : 2022-04-13 DOI: 10.1080/21623945.2022.2062852
Jie Pan, S. Kothan, Aye Thidar Moe Moe, Kun Huang
ABSTRACT The mechanism of insulin signaling on browning of white preadipocytes remains unclear. Human and mouse primary subcutaneous white preadipocytes (hsASCs and WT lean and obese msASCs, respectively) were induced to transdifferentiate into beige adipocytes under conditions of intact or blocked insulin signaling, respectively. Level of phosphoinositide-3-kinase (PI3K) after induction of beige adipocytes under conditions of normal insulin signaling, phosphorylated protein kinase B (pAKT), peroxisome proliferator-activated receptor γ coactivator-1 alpha (PGC-1α), zinc-fifinger transcriptional factor PRD1-BF1-RIZ1 homologous domain-containing protein 16 (PRDM16), uncoupling protein 1 (UCP1), peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer binding protein beta (C/EBPβ) were significantly increased. Conversely, when insulin signaling is incompletely inhibited, the expression of the thermogenic and adipogenic factors is significantly reduced, with obvious impairment of adipogenesis. However, phosphorylation level of adenosine 5’-monophosphate (AMP)-activated protein kinase (AMPK) and expression level of sirtuin type 1 (SIRT1) had increased. These white preadipocytes from different donors showed similar dynamic change in morphology and molecular levels during the browning. The present data indicate that insulin signaling plays a important role in regulation of browning of hsASCs and msASCs through PI3K-AKT-UCP1 signaling pathway. The insulin-AMPK-SIRT1 pathway was also involved in the adipocytes browning, while its effect is limited.
胰岛素信号在白色前脂肪细胞褐变中的作用机制尚不清楚。在胰岛素信号完整或阻断的条件下,分别诱导人和小鼠原代皮下白色前脂肪细胞(分别为hsASCs和WT瘦型和肥胖型msASCs)转分化为米色脂肪细胞。在正常胰岛素信号传导、磷酸化蛋白激酶B(pAKT)、过氧化物酶体增殖物激活受体γ共激活因子-1α(PGC-1α)、含锌转录因子PRD1-BF1-RIZ1同源结构域蛋白16(PRDM16)、解偶联蛋白1(UCP1)、,过氧化物酶体增殖物激活受体γ(PPARγ)和CCAAT/增强子结合蛋白β(C/EBPβ)显著增加。相反,当胰岛素信号传导被不完全抑制时,产热和脂肪因子的表达显著降低,脂肪生成明显受损。然而,5’-单磷酸腺苷(AMP)激活蛋白激酶(AMPK)的磷酸化水平和1型SIRT1的表达水平增加。这些来自不同供体的白色前脂肪细胞在褐变过程中表现出相似的形态和分子水平的动态变化。目前的数据表明,胰岛素信号通过PI3K-AKT-UCP1信号通路在调节hsASCs和msASCs的褐变中起着重要作用。胰岛素-AMPK-SIRT1通路也参与了脂肪细胞的褐变,但其作用有限。
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引用次数: 3
Integrative analyses of hub genes and their association with immune infiltration in adipose tissue, liver tissue and skeletal muscle of obese patients after bariatric surgery 肥胖患者减肥手术后脂肪组织、肝组织和骨骼肌中枢基因及其与免疫浸润的关联分析
IF 3.3 4区 生物学 Q2 Medicine Pub Date : 2022-04-12 DOI: 10.1080/21623945.2022.2060059
Kemin Yan, Pengyuan Zhang, Jiewen Jin, Xin Chen, H. Guan, Yanbing Li, Hai Li
ABSTRACT Bariatric surgery (BS) is an effective treatment for obesity. Adipose tissue, liver tissue and skeletal muscle are important metabolic tissues. This study investigated hub genes and their association with immune infiltration in these metabolic tissues of obese patients after BS by bioinformatic analysis with Gene Expression Omnibus datasets. Differentially expressed genes (DEGs) were identified, and a protein–protein interaction network was constructed to identify hub genes. As a result, 121 common DEGs were identified and mainly enriched in cytokine–cytokine receptor interactions, chemokine signaling pathway, neutrophil activation and immune responses. Immune cell infiltration analysis showed that the abundance of M1 macrophages was significantly lower in adipose and liver tissue after BS (p<0.05). Ten hub genes (TYROBP, TLR8, FGR, NCF2, HCK, CCL2, LAPTM5, MNDA and S100A9) that were all downregulated after BS were also associated with immune cells. Consistently, results in the validated dataset showed that the expression levels of these hub genes were increased in obese patients and mice, and decreased after BS. In conclusion, this study analysed the potential immune and inflammatory mechanisms of BS in three key metabolic tissues of obese patients, and revealed hub genes associated with immune cell infiltration, thus providing potential targets for obesity treatment.
减肥手术(BS)是治疗肥胖的有效方法。脂肪组织、肝组织和骨骼肌是重要的代谢组织。本研究利用Gene Expression Omnibus数据集,通过生物信息学分析肥胖症患者BS后这些代谢组织中的枢纽基因及其与免疫浸润的关系。鉴定差异表达基因(DEGs),构建蛋白互作网络鉴定枢纽基因。结果鉴定出121个常见的DEGs,主要富集于细胞因子-细胞因子受体相互作用、趋化因子信号通路、中性粒细胞活化和免疫应答。免疫细胞浸润分析显示,BS后脂肪组织和肝脏组织中M1巨噬细胞丰度显著降低(p<0.05)。10个中心基因(TYROBP、TLR8、FGR、NCF2、HCK、CCL2、LAPTM5、MNDA和S100A9)在BS后均下调,也与免疫细胞相关。验证数据集中的结果一致显示,肥胖患者和小鼠中这些枢纽基因的表达水平升高,BS后表达水平下降。综上所述,本研究分析了肥胖患者三个关键代谢组织中BS的潜在免疫和炎症机制,揭示了与免疫细胞浸润相关的枢纽基因,为肥胖治疗提供了潜在靶点。
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引用次数: 2
Transcriptome analysis of adipocytokines and their-related LncRNAs in lung adenocarcinoma revealing the association with prognosis, immune infiltration, and metabolic characteristics 肺腺癌中脂肪细胞因子及其相关lncrna的转录组分析揭示了与预后、免疫浸润和代谢特征的关联
IF 3.3 4区 生物学 Q2 Medicine Pub Date : 2022-04-11 DOI: 10.1080/21623945.2022.2064956
Jie Ren, Hui Zhang, Jinna Wang, Yi-yi Xu, Lei Zhao, Q. Yuan
ABSTRACT Lung adenocarcinoma (LUAD) is amongst the major contributors to cancer-related deaths on a global scale. Adipocytokines and long non-coding RNAs (lncRNAs) are indispensable participants in cancer. We performed a pan-cancer analysis of the mRNA expression, single nucleotide variation, copy number variation, and prognostic value of adipocytokines. LUAD samples were obtained from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases. Simultaneously, train, internal and external cohorts were grouped. After a stepwise screening of optimized genes through least absolute shrinkage and selection operator regression analysis, random forest algorithm,, and Cox regression analysis, an adipocytokine-related prognostic signature (ARPS) with superior performance compared with four additional well-established signatures for survival prediction was constructed. After determination of risk levels, the discrepancy of immune microenvironment, immune checkpoint gene expression, immune subtypes, and immune response in low- and high-risk cohorts were explored through multiple bioinformatics methods. Abnormal pathways underlying high- and low-risk subgroups were identified through gene set enrichment analysis (GSEA). Immune-and metabolism-related pathways that were correlated with risk score were selected through single sample GSEA. Finally, a nomogram with satisfied predictive survival probability was plotted. In summary, this study offers meaningful information for clinical treatment and scientific investigation.
肺腺癌(LUAD)是全球范围内导致癌症相关死亡的主要原因之一。脂肪细胞因子和长链非编码rna (lncRNAs)是癌症发生过程中不可或缺的参与者。我们对脂肪细胞因子的mRNA表达、单核苷酸变异、拷贝数变异和预后价值进行了泛癌分析。LUAD样本来自Gene Expression Omnibus (GEO)和the Cancer Genome Atlas (TCGA)数据库。同时,对培训、内部和外部队列进行分组。通过最小绝对收缩和选择算子回归分析、随机森林算法和Cox回归分析逐步筛选优化基因后,构建了一个与其他四个已建立的生存预测特征相比性能优越的脂肪细胞因子相关预后特征(ARPS)。在确定风险水平后,通过多种生物信息学方法探讨低、高危人群免疫微环境、免疫检查点基因表达、免疫亚型和免疫应答的差异。通过基因集富集分析(GSEA)确定了高风险和低风险亚组的异常途径。通过单样本GSEA选择与风险评分相关的免疫和代谢相关途径。最后,绘制具有满意预测生存概率的nomogram。本研究为临床治疗和科学研究提供了有意义的信息。
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引用次数: 20
The combination of nuclear receptor NR1D1 and ULK1 promotes mitophagy in adipocytes to ameliorate obesity 核受体NR1D1和ULK1的组合促进脂肪细胞的线粒体自噬以改善肥胖
IF 3.3 4区 生物学 Q2 Medicine Pub Date : 2022-04-11 DOI: 10.1080/21623945.2022.2060719
Bo Yu, Jindun Pan, F. Yu
ABSTRACT Obesity is a severe disease worldwide. Mitochondrial autophagy (mitophagy) may be related to metabolic abnormalities in obese individuals, but the mechanism is still unclear. We aimed to investigate whether nuclear receptors NR1D1 and ULK1 influence obesity by affecting mitophagy. In vitro model was established by inducing 3T3-L1 cells differentiation. MTT was detected cell viability. ELISA was tested triglyceride (TG). Oil red O staining was performed to detect lipid droplets. Flow cytometry was measured mtROS. ChIP and Dual-luciferase reporter assay were verified NR1D1 bind to ULK1. LC3 level was detected by IF. After differentiation medium treatment, cell viability was decreased, TG content and lipid droplets were increased Moreover, NR1D1 expression was reduced in Model group. NR1D1 overexpression was increased cell viability, reduced TG content and lipid droplets. Subsequently, NR1D1 inhibited TOM20 and mtROS, whereas, Parkin and PINK1 were accelerated. NR1D1 overexpression facilitated LC3 expression, whereas ULK1 knockdown was reversed the effect of NR1D1 overexpression. Liensinine also reversed the effect of NR1D1 overexpression, that is, cell viability was reduced, mtROS, TG content and lipid droplets were increased. The combination of nuclear receptor NR1D1 and ULK1 promoted mitophagy in adipocytes to alleviate obesity, which provided new target and strategy for obesity treatment. Abbreviations: Mitochondrial autophagy (mitophagy), triglyceride (TG), Uncoordinated-51 like autophagy activating kinase 1 (ULK1), Nuclear receptor subfamily 1 group D member 1 (NR1D1), American Type Culture Collection (ATCC), fetal bovine serum (FBS), 3-isobutyl-1-methylxanthine (IBMX), dexamethasone (DEX), short hairpin RNA ULK1 (sh-ULK1), wild-type (WT), mutant (MUT), Enzyme-linked immunosorbent assay (ELISA), mitochondrial reactive oxygen species (mtROS), Chromatin immunoprecipitation (ChIP), Quantitative real-time PCR (qRT-PCR), Immunofluorescence (IF), standard deviation (SD).
摘要肥胖是一种全球性的严重疾病。线粒体自噬可能与肥胖个体的代谢异常有关,但其机制尚不清楚。我们的目的是研究核受体NR1D1和ULK1是否通过影响线粒体自噬来影响肥胖。通过诱导3T3-L1细胞分化建立体外模型。MTT法检测细胞活力。ELISA法检测甘油三酯(TG)。进行油红O染色以检测脂滴。流式细胞术检测线粒体活性氧。ChIP和双荧光素酶报告基因测定证实NR1D1与ULK1结合。通过IF检测LC3水平。分化培养基处理后,模型组细胞活力下降,TG含量和脂滴增加,NR1D1表达降低。NR1D1过表达增加了细胞活力,降低了TG含量和脂滴。随后,NR1D1抑制TOM20和mtROS,而Parkin和PINK1加速。NR1D1过表达促进了LC3的表达,而ULK1敲低则逆转了NR1D1过度表达的作用。Liensinine还逆转了NR1D1过表达的作用,即细胞活力降低,mtROS、TG含量和脂滴增加。核受体NR1D1和ULK1的结合促进脂肪细胞的线粒体自噬以减轻肥胖,为肥胖治疗提供了新的靶点和策略。缩写:线粒体自噬(线粒体自噬)、甘油三酯(TG)、非配位-51样自噬激活激酶1(ULK1)、核受体亚家族1组D成员1(NR1D1)、美国典型培养物保藏中心(ATCC)、胎牛血清(FBS)、3-异丁基-1-甲基黄嘌呤(IBMX)、地塞米松(DEX)、短发夹RNA ULK1(sh-ULK1)、野生型(WT)、突变体(MUT),酶联免疫吸附试验(ELISA)、线粒体活性氧(mtROS)、染色质免疫沉淀(ChIP)、实时定量PCR(qRT-PCR)、免疫荧光(IF)、标准差(SD)。
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引用次数: 2
An organoid model derived from human adipose stem/progenitor cells to study adipose tissue physiology 从人脂肪干细胞/祖细胞衍生的类器官模型用于研究脂肪组织生理学
IF 3.3 4区 生物学 Q2 Medicine Pub Date : 2022-03-17 DOI: 10.1080/21623945.2022.2044601
M. Mandl, H. Viertler, Florian M Hatzmann, Camille Brucker, Sonja Großmann, Petra Waldegger, T. Rauchenwald, M. Mattesich, M. Zwierzina, G. Pierer, W. Zwerschke
ABSTRACT We established a functional adipose organoid model system for human adipose stem/progenitor cells (ASCs) isolated from white adipose tissue (WAT). ASCs were forced to self-aggregate by a hanging-drop technique. Afterwards, spheroids were transferred into agar-coated cell culture dishes to avoid plastic-adherence and dis-aggregation. Adipocyte differentiation was induced by an adipogenic hormone cocktail. Morphometric analysis revealed a significant increase in organoid size in the course of adipogenesis until d 18. Whole mount staining of organoids using specific lipophilic dyes showed large multi- and unilocular fat deposits in differentiated cells indicating highly efficient differentiation of ASCs into mature adipocytes. Moreover, we found a strong induction of the expression of key adipogenesis and adipocyte markers (CCAAT/enhancer-binding protein (C/EBP) β, peroxisome proliferator-activated receptor (PPAR) γ, fatty acid-binding protein 4 (FABP4), adiponectin) during adipose organoid formation. Secreted adiponectin was detected in the cell culture supernatant, underscoring the physiological relevance of mature adipocytes in the organoid model. Moreover, colony formation assays of collagenase-digested organoids revealed the maintenance of a significant fraction of ASCs within newly formed organoids. In conclusion, we provide a reliable and highly efficient WAT organoid model, which enables accurate analysis of cellular and molecular markers of adipogenic differentiation and adipocyte physiology.
摘要:我们建立了从白色脂肪组织(WAT)中分离的人脂肪干细胞/祖细胞(ASCs)的功能性脂肪类器官模型系统。通过悬滴技术迫使ASCs自聚集。之后,将球体转移到琼脂包被的细胞培养皿中,以避免塑料粘附和解聚。脂肪细胞分化是由脂肪生成激素鸡尾酒诱导的。形态计量学分析显示,在脂肪形成过程中,直到18 d,类器官的大小显著增加。用特异性亲脂性染料对类器官进行全载染色,发现分化细胞内有大量的多室和单室脂肪沉积,表明ASCs向成熟脂肪细胞的高效分化。此外,我们还发现,在脂肪类器官形成过程中,关键脂肪生成和脂肪细胞标志物(CCAAT/增强子结合蛋白(C/EBP) β、过氧化物酶体增殖物激活受体(PPAR) γ、脂肪酸结合蛋白4 (FABP4)、脂联素)的表达受到强烈诱导。在细胞培养上清中检测到分泌的脂联素,强调了成熟脂肪细胞在类器官模型中的生理相关性。此外,胶原酶消化的类器官的集落形成试验显示,在新形成的类器官中维持了相当一部分ASCs。总之,我们提供了一个可靠和高效的WAT类器官模型,可以准确分析脂肪形成分化和脂肪细胞生理学的细胞和分子标记。
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引用次数: 9
Adipose tissue aging partially accounts for fat alterations in HIV lipodystrophy 脂肪组织老化部分解释了HIV脂肪营养不良中脂肪的改变
IF 3.3 4区 生物学 Q2 Medicine Pub Date : 2022-03-17 DOI: 10.1080/21623945.2022.2042962
P. Domingo, M. Giralt, A. Gavaldà-Navarro, A. Blasco-Roset, Alejandro Delgado-Anglés, J. M. Gallego-Escuredo, M. Gutiérrez, M. Mateo, R. Cereijo, J. Domingo, F. Villarroya, J. Villarroya
ABSTRACT Lipodystrophy is a major disturbance in people living with HIV-1 (PLWH). Several systemic alterations in PLWH are reminiscent of those that occur in ageing. It is unknown whether the lipodystrophy in PLWH is the consequence of accelerated ageing in adipose tissue. We compared systemic and adipose tissue disturbances in PLWH with those in healthy elderly individuals (~80 y old). We observed similarly enhanced expression of inflammation-related genes and decreased autophagy in adipose tissues from elderly individuals and PLWH. Indications of repressed adipogenesis and mitochondrial dysfunction were found specifically in PLWH, whereas reduced telomere length and signs of senesce were specific to elderly individuals. We conclude that ageing of adipose tissue accounts only partially for the alterations in adipose tissues of PLWH.
脂肪营养不良是HIV-1 (PLWH)患者的主要障碍。PLWH的一些系统性改变与衰老过程中发生的变化相似。目前尚不清楚PLWH中的脂肪营养不良是否是脂肪组织加速老化的结果。我们比较了PLWH患者与健康老年人(~80岁)的全身和脂肪组织紊乱。我们在老年人和PLWH的脂肪组织中观察到类似的炎症相关基因表达增强和自噬减少。脂肪生成抑制和线粒体功能障碍的迹象是PLWH特有的,而端粒长度减少和衰老的迹象是老年人特有的。我们得出结论,脂肪组织的老化仅部分解释了PLWH脂肪组织的改变。
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引用次数: 1
Favorable fatty acid composition in adipose tissue in healthy Iraqi- compared to Swedish-born men — a pilot study using MRI assessment 健康伊拉克男性与瑞典出生男性脂肪组织中有利的脂肪酸成分——一项使用MRI评估的初步研究
IF 3.3 4区 生物学 Q2 Medicine Pub Date : 2022-03-16 DOI: 10.1080/21623945.2022.2042963
L. Trinh, Karin G. Stenkula, L. Olsson, J. Svensson, P. Peterson, L. Bennet, S. Månsson
ABSTRACT Middle Eastern immigrants are at high-risk for insulin resistance. Fatty acid composition (FAC) plays an important role in the development of insulin resistance but has not been investigated in people of Middle Eastern ancestry. Here, the aim was to assess the FAC in visceral and subcutaneous adipose tissue (VAT and SAT) in healthy Iraqi- and Swedish-born men using a magnetic resonance imaging (MRI) method.This case-control study included 23 Iraqi- and 15 Swedish-born middle-aged men, without cardiometabolic disease. Using multi-echo MRI of the abdomen, the fractions of saturated, monounsaturated, and polyunsaturated fatty acids (fSFA, fMUFA, and fPUFA) were estimated in VAT and SAT. SAT was further analyzed in deep and superficial compartments (dSAT and sSAT). In all depots, fPUFA was significantly higher and fSFA significantly lower in Iraqi men, independently of age and BMI. In both Iraqi- and Swedish-born men, higher fPUFA and lower fMUFA were found in sSAT vs. dSAT. Among Iraqi men only, higher fPUFA and lower fMUFA were found in SAT vs. VAT.Iraqi-born men presented a more favorable abdominal FAC compared to Swedish-born men. This MRI method also revealed different FACs in different abdominal depots. Our results may reflect a beneficial FAC in Middle Eastern immigrants.
中东移民是胰岛素抵抗的高危人群。脂肪酸组成(FAC)在胰岛素抵抗的发展中起重要作用,但尚未在中东血统人群中进行调查。在这里,目的是使用磁共振成像(MRI)方法评估伊拉克和瑞典出生的健康男性内脏和皮下脂肪组织(VAT和SAT)中的FAC。这项病例对照研究包括23名伊拉克和15名瑞典出生的中年男性,没有心脏代谢疾病。通过腹部多回波MRI,估计VAT和SAT中饱和、单不饱和和多不饱和脂肪酸(fSFA、fMUFA和fPUFA)的含量。进一步分析深层和浅表隔室(dSAT和sSAT)的SAT。在所有仓库中,伊拉克男性的fPUFA显著较高,fSFA显著较低,与年龄和BMI无关。在伊拉克和瑞典出生的男性中,sSAT与dSAT相比,fPUFA较高,fMUFA较低。仅在伊拉克男性中,在SAT和VAT中发现较高的fPUFA和较低的fMUFA。伊拉克出生的男性比瑞典出生的男性表现出更有利的腹部FAC。该MRI方法在腹部不同部位显示不同的FACs。我们的结果可能反映了中东移民中有益的FAC。
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引用次数: 1
T-stage-specific abdominal visceral fat, haematological nutrition indicators and inflammation as prognostic factors in patients with clear renal cell carcinoma 明确肾细胞癌患者t期特异性腹部内脏脂肪、血液学营养指标和炎症作为预后因素
IF 3.3 4区 生物学 Q2 Medicine Pub Date : 2022-03-12 DOI: 10.1080/21623945.2022.2048546
Hao Guo, Yumei Zhang, Heng Ma, Peiyou Gong, Yinghong Shi, Wenlei Zhao, Ai-yih Wang, Ming Liu, Zehua Sun, Fang Wang, Qing Wang, Xinru Ba
ABSTRACT Clear cell renal carcinoma (ccRCC) is the most common histological type of renal cancer and has the highest mortality. Several studies have been conducted on the relationship between adipose tissue and ccRCC prognosis, however, the results have been inconsistent to date. The current study aimed at establishing a link between abdominal fat composition and short-term prognosis in patients with ccRCC after T-stage stratification. We retrospectively analysed 250 patients with pathologically confirmed ccRCC (173 low T-stage and 77 high T-stage) in our hospital. The computed tomography (CT) images were evaluated using ImageJ. Then, subcutaneous and visceral fat areas (SFA and VFA), total fat areas (TFA) and the relative VFA (rVFA) were measured and computed. Meanwhile, biochemical indices of blood serum were analysed. The results showed that rVFA in low T-stage cohort who had a history of short-term postoperative complications were significantly lower than those who did not. No such association was observed in the high T-stage cohort. Further investigation revealed that the correlations between biochemical indexes and fat area-related variables varied across T-stage groups. As a result, rVFA is a reliable independent predictor of short-term prognosis in patients with low T-stage ccRCC but not in patients with high T-stage ccRCC.
透明细胞肾癌(ccRCC)是肾癌中最常见的组织学类型,死亡率最高。一些关于脂肪组织与ccRCC预后关系的研究已经开展,然而,迄今为止,结果并不一致。目前的研究旨在建立腹部脂肪组成与t期分层后ccRCC患者短期预后之间的联系。我们回顾性分析了我院250例经病理证实的ccRCC患者(173例低t期,77例高t期)。使用ImageJ对CT图像进行评价。然后测量并计算皮下和内脏脂肪面积(SFA和VFA)、总脂肪面积(TFA)和相对脂肪面积(rVFA)。同时对血清生化指标进行分析。结果显示有术后短期并发症史的低t期队列rVFA明显低于无术后短期并发症史的低t期队列rVFA。在高t期队列中没有观察到这种关联。进一步的研究发现,生化指标与脂肪面积相关变量之间的相关性在不同的t期组中有所不同。因此,rVFA是低t期ccRCC患者短期预后的可靠独立预测因子,而不是高t期ccRCC患者的预测因子。
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引用次数: 1
Fat grafting and platelet-rich plasma in wound healing: a review of histology from animal studies. 脂肪移植和富血小板血浆在伤口愈合中的应用:动物研究的组织学综述。
IF 3.3 4区 生物学 Q2 Medicine Pub Date : 2021-12-01 DOI: 10.1080/21623945.2021.1876374
Grant S Nolan, Oliver J Smith, Gavin Jell, Afshin Mosahebi

Stem cells could form the basis of a novel, autologous treatment for chronic wounds like diabetic foot ulcers. Fat grafts contain adipose-derived stem cells (ADSC) but low survival of cells within the grafts is a major limitation. Platelet-rich plasma (PRP) may increase graft survival. This review examines the histology from animal studies on fat grafting, ADSC and PRP in wound healing. A literature review of major electronic databases was undertaken, and narrative synthesis performed. Data from 30 animal studies were included. ADSC increase angiogenesis over 14 days and often clinically accelerated wound healing. ADSC had a greater effect in animals with impaired wound healing (e.g. diabetes). Activated PRP increased viability of fat grafts. Despite the high number of studies, the quality is variable which weakens the evidence. It does suggest there is a benefit of ADSC, particularly in impaired wound healing. High-quality evidence in humans is required, to establish its clinical usefulness.

干细胞可能成为一种新型的自体治疗慢性伤口的基础,比如糖尿病足溃疡。脂肪移植物含有脂肪源性干细胞(ADSC),但移植物内细胞存活率低是一个主要限制。富血小板血浆(PRP)可能增加移植物存活。本文综述了脂肪移植、ADSC和PRP在伤口愈合中的动物组织学研究。对主要电子数据库进行了文献回顾,并进行了叙述综合。数据来自30项动物研究。ADSC增加血管生成超过14天,通常在临床上加速伤口愈合。ADSC对伤口愈合受损的动物(如糖尿病)有更大的作用。活化的PRP增加了脂肪移植物的生存能力。尽管有大量的研究,但质量是可变的,这削弱了证据。它确实表明ADSC有好处,特别是在受损的伤口愈合方面。需要高质量的人体证据来确定其临床有效性。
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引用次数: 15
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Adipocyte
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