Murashige and Skoog (MS) medium supplemented with plant growth regulators composed of different concentrations were used in vitro rapid and mass multiplication of nodal explants obtained from shoots of black mulberry (Morus nigra L.) seedlings grown in a fully controlled greenhouse. Eighteen different concentration of 6-benzylaminopurin, thidiazuron (TDZ), kinetin (KN) GA3 and naphthalene acetic acid (NAA) as plant growth regulators were used. In contrast to expectation, combinations of (TDZ 1.0 mg L–1 + GA3 0.25 mg L–1) and (TDZ 2.0 mg L–1 + GA3 0.25 mg L–1) were found to give positive results in shoot proliferation and plant formation at the end of the study. In the rooting study (NAA 0.5 mg L–1 + KN 0.1 mg L–1) and (NAA 0.5 mg L–1 + KN 0.2 mg L–1) treatment showed positive results.
{"title":"Establishing an effective protocol for micropropagation of mullberry (Morus nigra L.)","authors":"Esra Bulunuz Palaz, R. Uğur","doi":"10.24326/asphc.2022.4.1","DOIUrl":"https://doi.org/10.24326/asphc.2022.4.1","url":null,"abstract":"Murashige and Skoog (MS) medium supplemented with plant growth regulators composed of different concentrations were used in vitro rapid and mass multiplication of nodal explants obtained from shoots of black mulberry (Morus nigra L.) seedlings grown in a fully controlled greenhouse. Eighteen different concentration of 6-benzylaminopurin, thidiazuron (TDZ), kinetin (KN) GA3 and naphthalene acetic acid (NAA) as plant growth regulators were used. In contrast to expectation, combinations of (TDZ 1.0 mg L–1 + GA3 0.25 mg L–1) and (TDZ 2.0 mg L–1 + GA3 0.25 mg L–1) were found to give positive results in shoot proliferation and plant formation at the end of the study. In the rooting study (NAA 0.5 mg L–1 + KN 0.1 mg L–1) and (NAA 0.5 mg L–1 + KN 0.2 mg L–1) treatment showed positive results.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44632250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Radomir, R. Stan, Mariana Letiția Pandelea, D. Vizitiu
The aim of this study was to elaborate an efficient in vitro multiplication protocol for Mentha piperita L. (peppermint) and to perform a comparative evaluation of some biochemical compounds in plants regenerated by micropropagation and conventional method. The use of a plain Murashige and Skoog (MS) basal medium favored the induction of regenerative processes, the percentage of explants that started to grow four weeks after inoculation being 92%. The highest multiplication rate (7.12 shoots/explant) and the highest average shoot length (8.11 cm) were obtained on the MS medium supplemented with 1 mg/L benzylaminopurine, when nodal fragments were used as explants. The rooting phase was not necessary, the shoots developing roots on the multiplication medium. The acclimatization rate of in vitro regenerated plants to ex vitro conditions was 96%. Although biochemical investigations revealed some differences between in vitro regenerated plants and those obtained by conventional methods, the results obtained show that micropropagation can be used successfully to obtain high-quality peppermint biological material, a potential source of bioactive compounds with therapeutic effect.
{"title":"In vitro multiplication of Mentha piperita L. and comparative evaluation of some biochemical compounds in plants regenerated by micropropagation and conventional method","authors":"A. Radomir, R. Stan, Mariana Letiția Pandelea, D. Vizitiu","doi":"10.24326/asphc.2022.4.5","DOIUrl":"https://doi.org/10.24326/asphc.2022.4.5","url":null,"abstract":"The aim of this study was to elaborate an efficient in vitro multiplication protocol for Mentha piperita L. (peppermint) and to perform a comparative evaluation of some biochemical compounds in plants regenerated by micropropagation and conventional method. The use of a plain Murashige and Skoog (MS) basal medium favored the induction of regenerative processes, the percentage of explants that started to grow four weeks after inoculation being 92%. The highest multiplication rate (7.12 shoots/explant) and the highest average shoot length (8.11 cm) were obtained on the MS medium supplemented with 1 mg/L benzylaminopurine, when nodal fragments were used as explants. The rooting phase was not necessary, the shoots developing roots on the multiplication medium. The acclimatization rate of in vitro regenerated plants to ex vitro conditions was 96%. Although biochemical investigations revealed some differences between in vitro regenerated plants and those obtained by conventional methods, the results obtained show that micropropagation can be used successfully to obtain high-quality peppermint biological material, a potential source of bioactive compounds with therapeutic effect.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45846397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the study was to evaluate the impact of silicon (Si) and calcium (Ca) added to the substrate (perliteor its mixture with peat) used in soilless strawberry cultivation on the number of different groups of microorganisms in the substrate. Research was conducted on a farm located in southern Poland in which soilless cultivation of strawberries in gutters, under covers, with an irrigation system was carried out. The microbiological analyzes were performed by serial dilution method. The analyzes included determination of the total number of bacteria, actinobacteria, fungi and aerobic atmospheric nitrogen assimilators of the Azotobacter genus. In this work, we showed that the concentration of microorganisms associated with the cultivation substrate may be influenced by the presence of silicon and calcium added to the composition of the substrate. Correlation analysis showed that the addition of Si + Ca to the substrate affects increase in the total number of bacteria in the substrate. The obtained results confirm that the cultivation substrate can be modified in such a way that it is more conducive to the multiplication and survival of bacteria associated with the substrate.
{"title":"The effect of silicon and calcium additives on the growth of selected groups of microorganisms in substrate used in soilless cultivation of strawberries","authors":"K. Frączek, Karol Bulski, T. Zaleski","doi":"10.24326/asphc.2022.4.6","DOIUrl":"https://doi.org/10.24326/asphc.2022.4.6","url":null,"abstract":"The aim of the study was to evaluate the impact of silicon (Si) and calcium (Ca) added to the substrate (perliteor its mixture with peat) used in soilless strawberry cultivation on the number of different groups of microorganisms in the substrate. Research was conducted on a farm located in southern Poland in which soilless cultivation of strawberries in gutters, under covers, with an irrigation system was carried out. The microbiological analyzes were performed by serial dilution method. The analyzes included determination of the total number of bacteria, actinobacteria, fungi and aerobic atmospheric nitrogen assimilators of the Azotobacter genus. In this work, we showed that the concentration of microorganisms associated with the cultivation substrate may be influenced by the presence of silicon and calcium added to the composition of the substrate. Correlation analysis showed that the addition of Si + Ca to the substrate affects increase in the total number of bacteria in the substrate. The obtained results confirm that the cultivation substrate can be modified in such a way that it is more conducive to the multiplication and survival of bacteria associated with the substrate.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42302070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The essential oil yield was obtained from the rosemary plant at the rate of 0.93 mL/100 g, and 1,8-cineol, camphor, isoborneol, α-pinene were identified as the highest component. While the total phenolic content in the essential oil of the rosemary plant was 13.87 mg GAE/g (DW) and the IC50 value was 15.02 μg extract mL–1, the DPPH antioxidant activity value was obtained as 38.43%. For the investigation of herbicidal effect of the essential oils on seed germination , different doses (0.5, 1.0, 2.0, 4.0, 8.0 and 16.0 μL Petri–1) were used on 2 crop plants (pepper and wheat) and 4 weeds: (Amaranthus palmeri S. Wats.), (Amaranthus albus L.), (Avena fatua L.) and (Sinapis arvensis L.). It was determined that as the applied amount of essential oil increased, the germination of the seeds was more suppressed. The highest effect of essential oil was detected in 16 μl Petri–1 dose application in all treated seeds.
{"title":"Chemical composition phenolic, antioxidant, and bio-herbicidal properties of the essential oil of rosemary (Rosmarinus officinalis L.)","authors":"Incilay Gokbulut, Yucel Karaman, A. Tursun","doi":"10.24326/asphc.2022.4.3","DOIUrl":"https://doi.org/10.24326/asphc.2022.4.3","url":null,"abstract":"The essential oil yield was obtained from the rosemary plant at the rate of 0.93 mL/100 g, and 1,8-cineol, camphor, isoborneol, α-pinene were identified as the highest component. While the total phenolic content in the essential oil of the rosemary plant was 13.87 mg GAE/g (DW) and the IC50 value was 15.02 μg extract mL–1, the DPPH antioxidant activity value was obtained as 38.43%. For the investigation of herbicidal effect of the essential oils on seed germination , different doses (0.5, 1.0, 2.0, 4.0, 8.0 and 16.0 μL Petri–1) were used on 2 crop plants (pepper and wheat) and 4 weeds: (Amaranthus palmeri S. Wats.), (Amaranthus albus L.), (Avena fatua L.) and (Sinapis arvensis L.). It was determined that as the applied amount of essential oil increased, the germination of the seeds was more suppressed. The highest effect of essential oil was detected in 16 μl Petri–1 dose application in all treated seeds.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45764966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-31DOI: 10.24326/asphc.2022.4.10
Łukasz Seliga, S. Pluta, A. Masny
The study was conducted in 2012–2014 at the Experimental Orchard of the National Institute of Horticultural Research in Skierniewice, central Poland. The aim of the research was to assess the breeding value, based on the general combining abilities (GCA), specific combining abilities (SCA) and reciprocal (RE) effects, of six dessert parental forms of blackcurrant for plant growth vigour, plant habit and resistance to fungal diseases: Podosphaera mors-uvae (causal agent of powdery mildew of currant), Drepanopeziza ribis (leaf anthracnose) and Cronartium ribicola (white pine blister rust). The plant material consisted of seedlings of F1 generation obtained by crossing of six blackcurrant genotypes: ‘Bona’, ‘Ceres’, clone D13B/11, ‘Sofiivska’, ‘Vernisazh’ and ‘Big Ben’ in a diallel design according to Griffing’s Method III.It was shown that the cultivars ‘Big Ben’, ‘Sofiivska’ and ‘Vernisazh’ had significantly positive GCA effects for growth vigour and resistance of plants to powdery mildew, whereas ‘Ceres’ – for plant habit, which indicated their high breeding value for these traits. The significantly positive SCA values were estimated for the hybrid family: ‘Big Ben’ × ‘Ceres’ for low susceptibility of plants to powdery mildew and anthracnose. Statistically significant effects for reciprocal crosses (RE) were obtained only for few hybrid families.
{"title":"Estimation of breeding value of blackcurrant genotypes for growth vigour, plant habit and their susceptibility to fungal diseases.","authors":"Łukasz Seliga, S. Pluta, A. Masny","doi":"10.24326/asphc.2022.4.10","DOIUrl":"https://doi.org/10.24326/asphc.2022.4.10","url":null,"abstract":"The study was conducted in 2012–2014 at the Experimental Orchard of the National Institute of Horticultural Research in Skierniewice, central Poland. The aim of the research was to assess the breeding value, based on the general combining abilities (GCA), specific combining abilities (SCA) and reciprocal (RE) effects, of six dessert parental forms of blackcurrant for plant growth vigour, plant habit and resistance to fungal diseases: Podosphaera mors-uvae (causal agent of powdery mildew of currant), Drepanopeziza ribis (leaf anthracnose) and Cronartium ribicola (white pine blister rust). The plant material consisted of seedlings of F1 generation obtained by crossing of six blackcurrant genotypes: ‘Bona’, ‘Ceres’, clone D13B/11, ‘Sofiivska’, ‘Vernisazh’ and ‘Big Ben’ in a diallel design according to Griffing’s Method III.It was shown that the cultivars ‘Big Ben’, ‘Sofiivska’ and ‘Vernisazh’ had significantly positive GCA effects for growth vigour and resistance of plants to powdery mildew, whereas ‘Ceres’ – for plant habit, which indicated their high breeding value for these traits. The significantly positive SCA values were estimated for the hybrid family: ‘Big Ben’ × ‘Ceres’ for low susceptibility of plants to powdery mildew and anthracnose. Statistically significant effects for reciprocal crosses (RE) were obtained only for few hybrid families.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43747056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.24326/asphc.2022.3.11
Anna Heród, M. Malik
Performance and appearance of vertical gardens are affected by plant quality, which is why creating conditions for their proper growth is crucial. Insufficient amount of light inside buildings significantly limits plant development, especially in the autumn and winter in the northern hemisphere. The objective of this study was to investigate physiological and morphological quality of plant species, Asplenium, Chlorophytum and Philodendron, in an indoor vertical garden exposed to two levels of daily light integral (DLI). Higher DLI level (1.1-1.7 mol m−2 day‑1) improved plant diameter, height, leaf length and width, leaf area, total area of leaf blades, dry weight, and carotenoid content, however did not affected leaf fresh weight and leaf number. Chlorophytum and Asplenium were particularly responsive to natural light supplementation and were distinguished by the best growth habit and compactness.
{"title":"EFFECT OF DAILY LIGHT INTEGRAL ON PHYSIOLOGICAL AND MORPHOLOGICAL QUALITY OF INDOOR PLANTS IN VERTICAL GARDEN","authors":"Anna Heród, M. Malik","doi":"10.24326/asphc.2022.3.11","DOIUrl":"https://doi.org/10.24326/asphc.2022.3.11","url":null,"abstract":"Performance and appearance of vertical gardens are affected by plant quality, which is why creating conditions for their proper growth is crucial. Insufficient amount of light inside buildings significantly limits plant development, especially in the autumn and winter in the northern hemisphere. The objective of this study was to investigate physiological and morphological quality of plant species, Asplenium, Chlorophytum and Philodendron, in an indoor vertical garden exposed to two levels of daily light integral (DLI). Higher DLI level (1.1-1.7 mol m−2 day‑1) improved plant diameter, height, leaf length and width, leaf area, total area of leaf blades, dry weight, and carotenoid content, however did not affected leaf fresh weight and leaf number. Chlorophytum and Asplenium were particularly responsive to natural light supplementation and were distinguished by the best growth habit and compactness.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44763071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Salinity is one of the most important abiotic stresses that affect plant cell metabolism and reduce plant productivity. In the study, some growth and biochemical characteristics of two different types of dwarf and lantern beans were investigated. The study was carried out in an aeroponic environment in a fully controlled climate room with 6 repetitions according to a completely randomized experimental design. In the experiment where two bean types (dwarf and pole types) were used as material, four different salt doses (0, 25, 50, 100 mM NaCl) were used for the salt stress conditions desired to be created. In the study, root length, seedling length, seedling fresh and dry weight, root fresh and dry weights, and leaf area were measured as some growth parameters, while MDA, APX, CAT, SOD activities, and proline and chlorophyll content were observed as biochemical parameters.For both genotypes, salinity induced a marked reduction in growth parameters. In parallel with the aggravation of salinity stress conditions, an increase in MDA, SOD, and proline content occurred. In the other investigated biochemical enzyme activities (APX and CAT), there was an increase up to a certain dose compared to the control and a decrease in the subsequent doses. Additionally, it has been determined that chlorophyll content is stable until the third dose but a significant decrease started after the fourth salt application. These results show that there has been a significant increase in enzymatic defense systems to reduce the effects of salt-induced stress in beans, and it has been observed that dwarf and pole bean types show close responses to salt stress.
盐度是影响植物细胞代谢和降低植物生产力的重要非生物胁迫之一。研究了矮豆和灯笼豆两种不同品种的生长和生化特性。本研究采用完全随机试验设计，在完全可控气候室内的气培环境中进行6次重复试验。试验以矮豆和扁豆两种类型为材料，采用4种不同盐剂量(0、25、50、100 mM NaCl)建立所需的盐胁迫条件。以根长、苗长、幼苗鲜干重、根鲜干重、叶面积为生长参数，以MDA、APX、CAT、SOD活性、脯氨酸和叶绿素含量为生化参数。对于两种基因型，盐度诱导生长参数显著降低。随着盐胁迫条件的加重，丙二醛、超氧化物歧化酶和脯氨酸含量增加。在其他研究的生化酶活性(APX和CAT)中，与对照相比，在一定剂量前有增加，随后剂量下降。此外，叶绿素含量在第三次施用前保持稳定，但在第四次施用后开始显著下降。这些结果表明，大豆酶防御系统显著增加，以减少盐胁迫的影响，并且已经观察到矮豆和极豆类型对盐胁迫表现出密切的响应。
{"title":"EFFECTS OF SALT STRESS ON SOME GROWTH PARAMETERS AND BIOCHEMICAL CHANGES IN BEAN (Phaseolus vulgaris L.)","authors":"Mustafa Çirka, R. Tunçtürk, H. Kulaz, M. Tunçtürk","doi":"10.24326/asphc.2022.3.5","DOIUrl":"https://doi.org/10.24326/asphc.2022.3.5","url":null,"abstract":"Salinity is one of the most important abiotic stresses that affect plant cell metabolism and reduce plant productivity. In the study, some growth and biochemical characteristics of two different types of dwarf and lantern beans were investigated. The study was carried out in an aeroponic environment in a fully controlled climate room with 6 repetitions according to a completely randomized experimental design. In the experiment where two bean types (dwarf and pole types) were used as material, four different salt doses (0, 25, 50, 100 mM NaCl) were used for the salt stress conditions desired to be created. In the study, root length, seedling length, seedling fresh and dry weight, root fresh and dry weights, and leaf area were measured as some growth parameters, while MDA, APX, CAT, SOD activities, and proline and chlorophyll content were observed as biochemical parameters.For both genotypes, salinity induced a marked reduction in growth parameters. In parallel with the aggravation of salinity stress conditions, an increase in MDA, SOD, and proline content occurred. In the other investigated biochemical enzyme activities (APX and CAT), there was an increase up to a certain dose compared to the control and a decrease in the subsequent doses. Additionally, it has been determined that chlorophyll content is stable until the third dose but a significant decrease started after the fourth salt application. These results show that there has been a significant increase in enzymatic defense systems to reduce the effects of salt-induced stress in beans, and it has been observed that dwarf and pole bean types show close responses to salt stress.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43077432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Feiying Huang, Li Tang, Xiaoming Wang, Neng Cai, Zhongquan Qiao
Different media had different effects on the induction of rooting of Lagerstroemia indica ‘Zijingling’. The aims of this study were to identify the best rooting medium, determine the changes in endogenous hormone content in aseptic seedlings of ‘Zijingling’, and then analyze its role in the rooting process, to improve the rooting rate. Using a test tube seedling of ‘Zijingling’ as the experimental material, the tissue cells were observed via paraffin sectioning, and the changes in endogenous hormone content during the rooting process were determined using high-performance liquid chromatography. The results showed that 1/2 MS Medium had the most significant effect on rooting in the basic medium. The promoting effects of different auxins on rooting decreased in magnitude in the order 3-Indolebutyric acid (IBA) > Naphthalene acetic acid (NAA)> Indole-3-aceticacid (IAA). The optimal rooting medium was 1/2 MS + 0.6 mg L-1 IBA+ 15 g L-1 sucrose + 5 g L-1 agar + 200 mg L-1 activated carbon , and the highest induction rate of adventitious roots was 92.5%. The rooting of ‘Zijingling’ is classified as a primordial type of induced rooting. Exogenous IBA content promoted an increase in endogenous IAA and Gibberellic acid (GA3) contents. High contents of IAA, GA3, and Abscisic acid (ABA) and low content of Zeatin riboside (ZR) promoted the growth of adventitious roots, whereas high contents of IAA and ZR, and low contents of GA3 and ABA were required for the induction of root primordia. High levels of IAA/ZR and low levels of IAA/ABA promoted the differentiation of root primordia. However, low levels of IAA/ZR and high levels of IAA/ABA promoted adventitious root elongation.
{"title":"STUDY ON in vitro INDUCTION OF ROOTING AND CHANGES IN ENDOGENOUS HORMONE CONTENT OF Lagerstroemia indica ‘ZIJINGLING’","authors":"Feiying Huang, Li Tang, Xiaoming Wang, Neng Cai, Zhongquan Qiao","doi":"10.24326/asphc.2022.3.4","DOIUrl":"https://doi.org/10.24326/asphc.2022.3.4","url":null,"abstract":"Different media had different effects on the induction of rooting of Lagerstroemia indica ‘Zijingling’. The aims of this study were to identify the best rooting medium, determine the changes in endogenous hormone content in aseptic seedlings of ‘Zijingling’, and then analyze its role in the rooting process, to improve the rooting rate. Using a test tube seedling of ‘Zijingling’ as the experimental material, the tissue cells were observed via paraffin sectioning, and the changes in endogenous hormone content during the rooting process were determined using high-performance liquid chromatography. The results showed that 1/2 MS Medium had the most significant effect on rooting in the basic medium. The promoting effects of different auxins on rooting decreased in magnitude in the order 3-Indolebutyric acid (IBA) > Naphthalene acetic acid (NAA)> Indole-3-aceticacid (IAA). The optimal rooting medium was 1/2 MS + 0.6 mg L-1 IBA+ 15 g L-1 sucrose + 5 g L-1 agar + 200 mg L-1 activated carbon , and the highest induction rate of adventitious roots was 92.5%. The rooting of ‘Zijingling’ is classified as a primordial type of induced rooting. Exogenous IBA content promoted an increase in endogenous IAA and Gibberellic acid (GA3) contents. High contents of IAA, GA3, and Abscisic acid (ABA) and low content of Zeatin riboside (ZR) promoted the growth of adventitious roots, whereas high contents of IAA and ZR, and low contents of GA3 and ABA were required for the induction of root primordia. High levels of IAA/ZR and low levels of IAA/ABA promoted the differentiation of root primordia. However, low levels of IAA/ZR and high levels of IAA/ABA promoted adventitious root elongation.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43783167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.24326/10.24326/asphc.2022.3.2
E. Kafkas, H. Gubbuk, H. Pınar, S. Selli, E. Gunes
Bananas have been grown in both open-field and protected cultivation in the subtropics. There are a very limited number of publications focusing on the impact of cultivation systems on the physico-chemical characteristics of bananas. For this reason, we assessed these fruit characteristics including sugars, malic acid, L-ascorbic acid, macro and micro nutrients well as fat and fatty acids of green and ripe bananas (Musa spp. AAA) in both cultivation systems. Experimental results have showed that many parameters affected the fruit ripening stage more than the cultivation system. Sucrose was the most abundant sugar followed by fructose and glucose in both the unripe and the ripe fruit stages. Sugar content, malic acid and L-ascorbic acid were higher in the ripe stage compared to the green stage. The cultivation system affected only glucose content, however, malic acid and L-ascorbic acid were not affected by the cultivation system. The most abundant macro and micro elements found were potassium and iron respectively. Cultivation system affected only potassium, zinc and manganese contents. Nitrogen and phosphorous, were found to be higher in the ripe stage. However, calcium, zinc, manganese and cupper were higher in the unripe stage. The fatty acid showed low value in the unripe stage for both cultivation systems. The concentration of MUFAs were found to be higher in the unripe stage for open-field condition while SFAs and PUFAs percentage were found to be higher in both cultivation systems. Experimental results clearly showed that physico-chemical characteristics of bananas were mainly affected by the ripening stage while the cultivation systems only affected a few characteristics.
{"title":"THE IMPACT OF OPEN-FIELD AND PROTECTED CULTIVATION ON BIOCHEMICAL CHARACTERISTICS OF BANANAS (Musa spp. AAA)","authors":"E. Kafkas, H. Gubbuk, H. Pınar, S. Selli, E. Gunes","doi":"10.24326/10.24326/asphc.2022.3.2","DOIUrl":"https://doi.org/10.24326/10.24326/asphc.2022.3.2","url":null,"abstract":"Bananas have been grown in both open-field and protected cultivation in the subtropics. There are a very limited number of publications focusing on the impact of cultivation systems on the physico-chemical characteristics of bananas. For this reason, we assessed these fruit characteristics including sugars, malic acid, L-ascorbic acid, macro and micro nutrients well as fat and fatty acids of green and ripe bananas (Musa spp. AAA) in both cultivation systems. Experimental results have showed that many parameters affected the fruit ripening stage more than the cultivation system. Sucrose was the most abundant sugar followed by fructose and glucose in both the unripe and the ripe fruit stages. Sugar content, malic acid and L-ascorbic acid were higher in the ripe stage compared to the green stage. The cultivation system affected only glucose content, however, malic acid and L-ascorbic acid were not affected by the cultivation system. The most abundant macro and micro elements found were potassium and iron respectively. Cultivation system affected only potassium, zinc and manganese contents. Nitrogen and phosphorous, were found to be higher in the ripe stage. However, calcium, zinc, manganese and cupper were higher in the unripe stage. The fatty acid showed low value in the unripe stage for both cultivation systems. The concentration of MUFAs were found to be higher in the unripe stage for open-field condition while SFAs and PUFAs percentage were found to be higher in both cultivation systems. Experimental results clearly showed that physico-chemical characteristics of bananas were mainly affected by the ripening stage while the cultivation systems only affected a few characteristics.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42238562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.24326/asphc.2022.3.12
Sadegh Azizifar, V. Abdossi, R. Gholami, M. Ghavami, A. Torkashvand
In this study, effect of salicylic acid (SA) and kaolin (KL) separately and simultaneously on yield, physiological traits and fatty acids composition of two olive cultivars (i.e. ‘Zard’ and ‘Roghani’) was investigated under three irrigation regimes. Results showed that deficit irrigation, especially 50% crop evapotranspiration (ETc), increased accumulation of MDA, proline and activity of antioxidant enzymes, but decreased chlorophyll content and yield in both the olive cultivars, as compared to full irrigation (100% ETc). Applying SA and KL alleviated the harmful effect of water deficit on plants through enhancing their chlorophyll content and antioxidant activity, and accordingly improved their yield, as compared to the controls. On the contrary, low irrigation mounted some fatty acids such as palmitoleic acid, stearic acid, arachidic acid, while reduced unsaturated/saturated fatty acids. Moreover, applying SA and KL simultaneously, as compared to the controls, increased unsaturated/saturated fatty acids and the quality of extracted olive oil. In general, the results showed that a simultaneous effect of SA and KL, in comparison to their separate application, had a better effect on yield and quality of olive under water deficit.
{"title":"EFFECT OF SALICYLIC ACID AND KAOLIN ON YIELD, PHYSIOLOGICAL TRAITS, AND FATTY ACID COMPOSITION IN OLIVE CULTIVARS UNDER REGULATED DEFICIT IRRIGATION","authors":"Sadegh Azizifar, V. Abdossi, R. Gholami, M. Ghavami, A. Torkashvand","doi":"10.24326/asphc.2022.3.12","DOIUrl":"https://doi.org/10.24326/asphc.2022.3.12","url":null,"abstract":"In this study, effect of salicylic acid (SA) and kaolin (KL) separately and simultaneously on yield, physiological traits and fatty acids composition of two olive cultivars (i.e. ‘Zard’ and ‘Roghani’) was investigated under three irrigation regimes. Results showed that deficit irrigation, especially 50% crop evapotranspiration (ETc), increased accumulation of MDA, proline and activity of antioxidant enzymes, but decreased chlorophyll content and yield in both the olive cultivars, as compared to full irrigation (100% ETc). Applying SA and KL alleviated the harmful effect of water deficit on plants through enhancing their chlorophyll content and antioxidant activity, and accordingly improved their yield, as compared to the controls. On the contrary, low irrigation mounted some fatty acids such as palmitoleic acid, stearic acid, arachidic acid, while reduced unsaturated/saturated fatty acids. Moreover, applying SA and KL simultaneously, as compared to the controls, increased unsaturated/saturated fatty acids and the quality of extracted olive oil. In general, the results showed that a simultaneous effect of SA and KL, in comparison to their separate application, had a better effect on yield and quality of olive under water deficit.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44710874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：