Acta tropica最新文献

{"title":"Evaluation of the immunogenicity of elephant endotheliotropic herpesvirus glycoprotein B (EEHV-gB) subunit vaccines in a mouse model","authors":"Tidaratt Sittisak ,&nbsp;Thunyamas Guntawang ,&nbsp;Saralee Srivorakul ,&nbsp;Kornravee Photichai ,&nbsp;Anucha Muenthaisong ,&nbsp;Amarin Rittipornlertrak ,&nbsp;Varankpicha Kochagul ,&nbsp;Naricha Khamluang ,&nbsp;Nattawooti Sthitmatee ,&nbsp;Phongsakorn Chuammitri ,&nbsp;Chatchote Thitaram ,&nbsp;Wei-Li Hsu ,&nbsp;Kidsadagon Pringproa","doi":"10.1016/j.actatropica.2025.107571","DOIUrl":"10.1016/j.actatropica.2025.107571","url":null,"abstract":"<div><div>Elephant endotheliotropic herpesvirus (EEHV), a persistent threat, has caused significant mortality among young Asian elephants (<em>Elephas maximus</em>), raising serious concerns for the conservation of this endangered species. Given the urgent need for protective measures, research into EEHV vaccine development has become increasingly critical. This study evaluated the immune response in mice following immunization with an EEHV1A-glycoprotein B (gB) subunit vaccine. The vaccine incorporated gBF1 and gBF2, corresponding to segments of the gB ectodomains I and IV, respectively, along with emulsion adjuvants Montanide™ ISA 206 VG and incomplete Freund's adjuvant (IFA). The findings revealed that both gBF1 and gBF2, when paired with these adjuvants, were capable of inducing strong humoral immune responses against EEHV-gB, as demonstrated by the ability of sera from immunized mice to detect EEHV-gB <em>ex vivo</em>. Additionally, in terms of cellular immunity, the vaccine formulations predominantly activated CD4+ T cells, including both Th1 (IFN-γ+) and Th2 (IL-4+) cells, with no significant activation of CD8+ T cells. If also applicable in elephants, gB-based vaccines would be a significant step forward in the fight against EEHV.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107571"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The proteome of human adult whipworm Trichuris trichiura: A source of potential immunomodulatory molecules","authors":"Leonardo F. Santiago ,&nbsp;Eduardo S. da Silva ,&nbsp;Priscila S. dos Santos ,&nbsp;Luis F. Salazar- Garcés ,&nbsp;Sara P.O. Santos ,&nbsp;Antônio M.S. Fernandes ,&nbsp;Raphael C. Silva ,&nbsp;Vitor S. Alves ,&nbsp;Peter Briza ,&nbsp;Fatima Ferreira ,&nbsp;Luis G.C. Pacheco ,&nbsp;Neuza M. Alcantara-Neves ,&nbsp;Carina S. Pinheiro","doi":"10.1016/j.actatropica.2025.107566","DOIUrl":"10.1016/j.actatropica.2025.107566","url":null,"abstract":"<div><div>Soil-transmitted helminths (STHs), including <em>Trichuris trichiura</em>, pose a major global health challenge, particularly in children, causing significant morbidity. However, <em>T. trichiura</em>'s ability to modulate host immune responses offers a unique opportunity to discover biomolecules with therapeutic potential for inflammatory, allergic, and autoimmune disorders. This study conducted a proteomic analysis of adult male and female <em>T. trichiura</em> using liquid chromatography-tandem mass spectrometry (LC-MS/MS), identifying 810 parasite proteins. Of these, 177 were exclusive to females, 277 to males, and 356 shared.</div><div>Gene ontology analysis showed similar cellular component profiles in males and females, mostly involving intracellular structures. However, female-exclusive proteins exhibited more diverse components. Molecular function analysis highlighted hydrolytic and catalytic activities, suggesting enzymatic strategies for nutrition and immune modulation. Notably, immunomodulatory proteins were identified in both sexes, showing therapeutic potential, including a Kunitz protease inhibitor and glutamate dehydrogenase.</div><div>To evaluate immunomodulatory properties, one identified protein (rc4299) was tested on cultures of peripheral blood mononuclear cells (PBMCs) from allergic individuals. The recombinantly produced rc4299 increased IL-10 secretion, indicating potential for treating autoimmune and allergic diseases. This study uncovers the <em>T. trichiura</em> proteome and highlights promising therapeutic targets, emphasizing the parasite's complex interactions with the host immune system.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107566"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential impact of climate change on Opisthorchis viverrini and Opisthorchis felineus transmission in Eurasia","authors":"Banchob Sripa ,&nbsp;Natalia Yurlova ,&nbsp;Apiporn T. Suwannatrai ,&nbsp;Elena Serbina ,&nbsp;Sirikachorn Tangkawattana ,&nbsp;Somphou Sayasone ,&nbsp;Pariwate Varnakovida","doi":"10.1016/j.actatropica.2025.107574","DOIUrl":"10.1016/j.actatropica.2025.107574","url":null,"abstract":"<div><div>Human liver flukes of the family Opisthorchiidae, particularly <em>Opisthorchis viverrini</em> and <em>Opisthorchis felineus</em>, are major foodborne trematode parasites endemic to the Lower Mekong River Basin (Southeast Asia) and the Ob-Irtysh Basins (southern Western Siberia, Russian Federation), respectively. Together, these parasites infect over 14 million people, with an estimated 300 million at risk. Their transmission cycles involve <em>Bithynia</em> snails and Cyprinidae fish as first and second intermediate hosts, respectively, with humans and other fish-eating mammals serving as definitive hosts. The geographical distribution of these flukes is shaped by specific <em>Bithynia</em> species: <em>B. siamensis, B.</em> s<em>. goniomphalos</em>, and <em>B. funiculata</em> for <em>O. viverrini</em>, and <em>B. troschelii, B. leachi</em>, and <em>B. inflata</em> for <em>O. felineus</em>. Climate change directly influences liver fluke transmission by affecting parasite survival, host biology, and environmental conditions. <em>Bithynia</em> snails are highly temperature-sensitive, and <em>O. viverrini</em> transmission is notably temperature-dependent, with a 1 °C increase raising infection odds by 5.4 %. Temperatures exceeding 30 °C reduce cercarial survival and infectivity. In Western Siberia, favorable water temperatures for O. felineus transmission start at +15 °C, with higher temperatures leading to an increased infection rate in snails while permafrost regions lack <em>Bithynia</em> snails entirely. Projections from the Intergovernmental Panel on Climate Change (IPCC) indicate that warming will exceed the global average in northern Asia (affecting <em>O. felineus</em>) and approach the global average in Southeast Asia (impacting <em>O. viverrini</em>). These trends suggest that climate change may have a more pronounced impact on <em>O. felineus</em> transmission in Siberia than on <em>O. viverrini</em> transmission in Southeast Asia. This review provides an in-depth discussion of <em>Bithynia</em> biology and the effects of temperature on snail growth, cercarial release, survival, infection, and aestivation, emphasizing how global warming could influence the transmission dynamics of these liver flukes.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107574"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Evaluation of Wolbachia infection in Aedes aegypti suggests low prevalence and highly heterogeneous distribution in Medellín, Colombia” [Acta Tropica, Volume 260, December 2024, 107423]","authors":"Arley Calle-Tobón ,&nbsp;Raúl Rojo-Ospina ,&nbsp;Sara Zuluaga ,&nbsp;Juan F. Giraldo-Muñoz ,&nbsp;Jorge Mario Cadavid","doi":"10.1016/j.actatropica.2025.107572","DOIUrl":"10.1016/j.actatropica.2025.107572","url":null,"abstract":"","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107572"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic toxoplasmosis induces depression-like behaviors and neuroinflammatory responses in mice","authors":"Wen-Qian Shi ,&nbsp;Shao-Yuan Bai ,&nbsp;Ming Pan ,&nbsp;Qi-Wang Jin ,&nbsp;Zuohua Liu ,&nbsp;Xinwen Bo ,&nbsp;Si-Yang Huang","doi":"10.1016/j.actatropica.2025.107568","DOIUrl":"10.1016/j.actatropica.2025.107568","url":null,"abstract":"<div><div>Approximately one-third of the global population is infected with <em>Toxoplasma gondii</em>, which can lead to chronic infection and induce a persistent inflammatory response. This inflammation is known to increase the risk of depression-like behaviors. To investigate the impact of chronic toxoplasmosis on depression, a model of chronic infection was established, and behavioral changes were evaluated using a series of tests, including the Tail Suspension Test (TST), Sucrose Preference Test (SPT), Olfactory Sensitivity Test (OST), and Tail Immersion Test (TIT). The results showed that chronically infected mice exhibited significant depression-like behaviors and impaired olfactory sensitivity, while their pain perception remained unaffected. Additionally, the levels of astrocytes and neuroinflammation in the mouse brain were further assessed. It was observed that the expression of GFAP and proinflammatory cytokines (TNF-α, IL-1β, IL-6, IDO) was upregulated. These findings suggest that chronic toxoplasmosis induces neuroinflammation and astrocyte activation, which may contribute to the manifestation of depression-like behaviors.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"264 ","pages":"Article 107568"},"PeriodicalIF":2.1,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of meteorological and socio-economic factors on malaria in mainland China (2006–2015): A study using spatiotemporal analysis and Bayesian model","authors":"Lili Liu ,&nbsp;Zhenyi Wang ,&nbsp;Yanni Tian ,&nbsp;Yiwen Tao ,&nbsp;Yazhi Li","doi":"10.1016/j.actatropica.2025.107554","DOIUrl":"10.1016/j.actatropica.2025.107554","url":null,"abstract":"<div><div>Nowadays malaria still is a significant and prevalent infectious disease in mainland China. How meteorological and socio-economic factors affect the distribution of malaria in mainland China? It is worthy to explore their impact on malaria to guide other serious regions. This study selects the total number of malaria cases included in both local and imported cases of malaria in mainland China from 2006 to 2015. According to integrating 6 meteorological and socio-economic factors, we use the spatiotemporal analysis and Bayesian model to quantify the impact of multi-factors on malaria. The results show that malaria is distributed in a northeast southwest direction, with hotspots mainly located in Shandong, Anhui, Jiangsu etc. Precipitation has a significant impact on the incidence of malaria, while socio-economic factors have no significant impact on the incidence of malaria. In addition, malaria in mainland China exhibits significant spatial heterogeneity, with significant differences in <span><math><mrow><mi>R</mi><mi>R</mi></mrow></math></span>(Relative Risk) of different regions. The related results aim to provide the theoretical guidance for formulating the prevention and control policy.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107554"},"PeriodicalIF":2.1,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA barcoding and host blood meal identification of Culicoides Latreille (Diptera, Ceratopogonidae) from Malaysia","authors":"Zubaidah Ya'cob ,&nbsp;Ronnalit Mintara ,&nbsp;Daicus M. Belabut ,&nbsp;Muhammed Rasul Abdullah Halim ,&nbsp;Pairot Pramual","doi":"10.1016/j.actatropica.2025.107564","DOIUrl":"10.1016/j.actatropica.2025.107564","url":null,"abstract":"<div><div>Mitochondrial cytochrome oxidase I (COI) sequences were used to determine the genetic diversity and species identification efficacy of <em>Culicoides</em> from Malaysia. In total, 100 COI sequences were obtained from 13 morphologically identified species. Intraspecific genetic divergence varied from 0.48 % in <em>C. parahumeralis</em> to 14.88 % in <em>C. palpifer</em>. However, most (8 of 13) had low (&lt;3 %) intraspecific genetic divergence. Identification of these species based on best match (BM) and best close match (BCM) methods revealed a high efficiency of the COI sequences with 100 % and 99 % success for BM and BCM, respectively. Identification in the BOLD database revealed that 10 species were successfully determined and agreed with morphological identifications. There remained three taxa which were ambiguous (<em>C. jacobsoni</em>) or had no species level identity match (<em>C. palpifer</em> and <em>C. flavescens</em>). Phylogenetic analyses found that all Malaysian specimens were clustered with conspecifics except <em>C. palpifer</em>. Specimens of this taxon separated into two divergent clades, one with members of a BIN (BOLD:ADT9601) of <em>C. palpifer</em> whereas the other formed a novel genetic lineage. Molecular species delimitation identified the morphologically identified species of the Malaysian <em>Culicoides</em> in the respective species. The exceptions were two divergent lineages found in <em>C. palpifer</em> because they were treated as two different taxa. Molecular identification of the host blood meal source revealed that all were from water buffalo (<em>Bubalus bubalis</em>).</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107564"},"PeriodicalIF":2.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology of bluetongue virus infection among goat populations in central and western Thailand: Prevalence, risk factors, and serotype analysis","authors":"Thanyaporn Homat ,&nbsp;Yuki Fujisawa ,&nbsp;Chowalit Nakthong ,&nbsp;Phirom Prompiram ,&nbsp;Rassmeepen Phonaknguen ,&nbsp;Tapanut Songkasupa ,&nbsp;Pratana Satitvipawee ,&nbsp;Kridsada Chaichoun ,&nbsp;Kripitch Sutummaporn ,&nbsp;Sudsaijai Kornmatitsuk ,&nbsp;Bunlue Kornmatitsuk","doi":"10.1016/j.actatropica.2025.107558","DOIUrl":"10.1016/j.actatropica.2025.107558","url":null,"abstract":"<div><div>The current status of bluetongue (BT) in Thailand remains unclear, and the serotypes of BT virus (BTV) have not been determined. This study aimed to 1) investigate the epidemiology of BTV infection among goat populations in central and western Thailand; 2) evaluate the test performance of conventional RT-PCR for BTV detection; and 3) identify the BTV serotypes presently circulating in the study area. The herd seroprevalence was 94.6 % (53/56, 95 % CI = 85.3–98.1), while the individual seroprevalence was 74.4 % (434/583, 95 % CI = 70.5–77.6). The odds of BTV infection were higher in goats in the western region (aOR = 10.16, 95 % CI = 5.02–20.55), with the presence of nearby ruminant farms (aOR = 2.21, 95 % CI = 1.23–4.00), and aged ≥3 years (aOR = 14.02, 95 % CI = 6.32–31.08) but lower in goats with an intensive rearing system (aOR = 0.18, 95 % CI = 0.09–0.39). For BTV detection, conventional RT-PCR presented 84.5 % sensitivity and 97.4 % specificity with an AUC of 0.910 (95 % CI = 0.852–0.967). Seven BTV serotypes (2, 3, 4, 7, 12, 16, and 21) were identified. Among these, BTV-2, 12, and 21 were the most prevalent, and BTV-4 was first detected in Southeast Asia. These results illustrated that BT is endemic and spreading widely among goat populations in the study area. Conventional RT-PCR developed in this study proved to be a reliable method for BTV detection. This research provides the first report of BTV serotype distribution in Thailand.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107558"},"PeriodicalIF":2.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melioidosis in people living with diabetes; clinical presentation, clinical course and implications for patient management","authors":"Laura Prideaux ,&nbsp;Megan Sandeman ,&nbsp;Hayley Stratton ,&nbsp;Anthony D Kelleher ,&nbsp;Simon Smith ,&nbsp;Josh Hanson","doi":"10.1016/j.actatropica.2025.107559","DOIUrl":"10.1016/j.actatropica.2025.107559","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;Despite the well-established link between diabetes mellitus and melioidosis, the precise impact of diabetes, its complications, and its therapy on the presentation and clinical course of melioidosis is incompletely defined. The influence of glycaemic control on the diverse clinical manifestations and the clinical course of melioidosis in patients with diabetes has also not been examined in detail.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;We examined all cases of culture-confirmed melioidosis in Far North Queensland, Australia between October 1, 2016, and April 30, 2024. We hoped to define the impact of diabetes, its control and its therapy on the patients’ presentation and their clinical course.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;There were 321 cases of culture-confirmed melioidosis during the study period; the patients’ median (interquartile range (IQR)) age was 57 (46–69) years, 212/321 (66 %) were male, 130/321 (41 %) identified as First Nations Australians. Diabetes was the most common risk factor for melioidosis in the cohort (163/321, 51 %); in 19/163 (12 %) this was a new diagnosis. The median (IQR)) glycosylated haemoglobin prior to presentation was 9.1 % (7.2–11.5) and 96/162 (59 %) with complete data had established macrovascular or microvascular complications. People with diabetes were more likely – than people without diabetes – to have involvement of the liver (odds ratio (OR) 95 % confidence interval (CI): 9.68 (2.21–42.46), &lt;em&gt;p&lt;/em&gt; = 0.003), the spleen (OR (95 % CI): 7.32 (1.64–32.80), &lt;em&gt;p&lt;/em&gt; = 0.009) or to have disseminated disease (OR (95 % CI): 2.93 (1.26–6.78), &lt;em&gt;p&lt;/em&gt; = 0.01). However, people with diabetes were no more likely than people without diabetes to require intensive care unit admission (OR (95 % CI): 0.82 (0.47–1.42), &lt;em&gt;p&lt;/em&gt; = 0.48) or to die before hospital discharge (12/163 (7 %) versus 19/158 (12 %), OR (95 % CI): 0.58 (0.27–1.24), &lt;em&gt;p&lt;/em&gt; = 0.16). Only 58/163 (36 %) with diabetes had specialist endocrinology review during their hospitalisation and only 22/72 (31 %) with accessible data had good glycaemic control (glycosylated haemoglobin ≤7 %) in the 12 months after discharge, increasing their risk of subsequent diabetic complications. Of the 151 people with diabetes surviving their hospitalisation, 26 (17 %) died, at a median (IQR) of 1.0 (0.40–4.1) years after discharge. Of the individuals with diabetes who had completed five years of follow up, 21/60 (35 %) had died at a median (IQR) age of 67 (51–84) years.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;div&gt;Individuals with diabetes and melioidosis are more likely to have liver and spleen abscesses and disseminated disease than individuals without diabetes, manifestations that appear to be linked directly to glycaemic control. In Australia's well-resourced health system &lt;10 % of patients with diabetes and melioidosis will die from their infection. However, five-year all-cause mortality in individuals with diabetes who survi","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107559"},"PeriodicalIF":2.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Wolbachia on mitochondrial DNA variation in Aedes albopictus (Diptera: Culicidae)","authors":"Zu Ma ,&nbsp;Jian Gao ,&nbsp;Ge Wang ,&nbsp;Minghui Zhao ,&nbsp;Dan Xing ,&nbsp;Tongyan Zhao ,&nbsp;Hengduan Zhang","doi":"10.1016/j.actatropica.2025.107561","DOIUrl":"10.1016/j.actatropica.2025.107561","url":null,"abstract":"<div><div><em>Wolbachia</em> species are symbiotic bacteria that are commonly found in arthropods and nematodes and live inside their cells. In nature, endosymbiont–host interactions and dynamics are complex, often depending on environmental conditions and evolutionary history. Both <em>Wolbachia</em> and mitochondrial DNA are maternally inherited in cells, and after a long period of coexistence, the presence of <em>Wolbachia</em> may have an impact on mitochondrial sequence diversity, thereby confounding mtDNA-based host phylogeny. The universal and typing primers for the <em>wsp</em> gene were used for PCR amplification, the number of positive samples was counted, and the infection pattern was analysed. The mitochondrial DNA diversity of four groups (<em>Wolbachia</em>-infected and uninfected samples, as well as between singly and double infected samples.) was analysed. PACo and ParaFitGlobal tests were used to explore evolutionary associations. The overall prevalence of <em>Wolbachia</em> in the 22 natural populations was 94.2 %, with Type A, Type B and <em>A</em> × <em>B</em> mixed infections detected in <em>Aedes albopictus</em> and coinfection between <em>w</em>AlbA and <em>w</em>AlbB prevalent. The mitochondrial DNA haplotype associated with <em>Wolbachia</em> (Hap1) became the dominant haplotype and was the most abundant and widely distributed in the population. The linkage map showed the predominant haplotype, Hap1, was more closely associated with <em>w</em>AlbA than with <em>w</em>AlbB. Neutral evolution deviated significantly from zero. The diversity of mtDNA COI genes associated with <em>Wolbachia</em> infection was reduced. <em>Wolbachia</em> infection may lead to the selective sweep of mitochondrial DNA in <em>Ae. albopictus</em>.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"263 ","pages":"Article 107561"},"PeriodicalIF":2.1,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}