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Reinforced optical cage systems enable drift-free single-molecule localization microscopy. 增强光学笼系统使无漂移的单分子定位显微镜。
Pub Date : 2025-12-15 DOI: 10.1038/s44172-025-00566-4
Hao Qiu, Matthew C Tang, Selene K Roberts, Guoli Li, Rong Su, Marisa L Martin-Fernandez, David T Clarke, Shugang Liu, Xiaojie Liu, Lin Wang

Single-molecule localization microscopy achieves nanometer-scale resolution but is compromised by sample drift during image acquisition. Here we present reinforced optical cage systems, a novel approach that eliminates drift at its mechanical source rather than correcting it through complex image post-processing or fiducial markers. Reinforced optical cage systems employ perforated optomechanical components interconnected by tungsten-steel rods in a design proven by mechanical stability simulations. Our bench-top microscope, built with reinforced optical cage systems, demonstrated exceptional three-dimensional stability, with mean cumulative lateral drift of approximately 5 nanometers over 2 h in widefield fluorescence microscopy and 11-16 nanometers over 15 min in single-molecule localization microscopy, free from measurable axial drift. This development allows super-resolution microscopy to reach its full resolution without the necessity of sample drift correction, offering a straightforward, cost-effective, low-maintenance, and readily accessible solution to high-performance super-resolution microscopy. By addressing the fundamental issue of mechanical instability, reinforced optical cage systems enable improved precision instrumentation for the broader scientific and engineering community.

单分子定位显微镜达到纳米级分辨率,但在图像采集过程中受到样品漂移的影响。在这里,我们提出了增强光学笼系统,这是一种新的方法,可以消除其机械源的漂移,而不是通过复杂的图像后处理或基准标记来纠正它。增强型光学笼系统采用由钨钢棒连接的穿孔光学机械部件,其设计经过机械稳定性模拟验证。我们的台式显微镜,采用强化光学笼系统,显示出卓越的三维稳定性,在宽视场荧光显微镜下,平均累积横向漂移约为5纳米/ 2小时,在单分子定位显微镜下,平均累积横向漂移约为11-16纳米/ 15分钟,没有可测量的轴向漂移。这一发展允许超分辨率显微镜达到其全分辨率,而无需样品漂移校正,为高性能超分辨率显微镜提供直接,经济高效,低维护和易于访问的解决方案。通过解决机械不稳定的基本问题,增强光学笼系统可以为更广泛的科学和工程界提供改进的精密仪器。
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引用次数: 0
Pneumatically controlled lattices with tunable mechanical behavior. 具有可调机械行为的气动控制格子。
Pub Date : 2025-12-13 DOI: 10.1038/s44172-025-00570-8
Xiaoheng Zhu, Yucong Hua, Dengge Jin, Jordan R Raney

Buckling is a common failure mode in lattice structures, limiting their use in some applications. The tendency of a strut to buckle is related to the local nodal connectivity. In this work, we introduce a pneumatic actuation strategy to actively tune the mechanical behavior of lattice structures by locally reconfiguring their effective nodal connectivity. By selectively inflating pneumatic actuators embedded in the lattice into spatial patterns with varying levels of connectivity, we demonstrate a method to modulate mechanical properties, including stiffness and buckling response. The most reinforced pattern can lead to 121.6% improvement in buckling strength relative to the regular lattice itself. Additionally, the post-buckling behavior of pneumatically controlled lattices can be programmably tuned by varying the input air pressure signals. The pneumatically controlled lattices reduced the peak acceleration by 50.9%, demonstrating enhanced impact mitigation capability. These results show that pneumatic actuation provides a versatile approach to enhancing structural performance under both static and dynamic loading. Since this strategy does not rely on multi-material interfaces or specific cell topologies, it can be broadly applied to optimize a wide range of lattice architectures.

屈曲是晶格结构中常见的失效模式,限制了其在某些应用中的应用。支撑的屈曲倾向与局部节点的连通性有关。在这项工作中,我们引入了一种气动驱动策略,通过局部重新配置其有效节点连接来主动调整晶格结构的力学行为。通过选择性地将嵌入晶格中的气动执行器充气成具有不同连接水平的空间模式,我们展示了一种调节机械性能的方法,包括刚度和屈曲响应。与常规晶格相比,强化程度最高的晶格屈曲强度提高了121.6%。此外,气动控制晶格的后屈曲行为可以通过改变输入的气压信号进行可编程调整。气动控制栅格将峰值加速度降低了50.9%,显示出增强的冲击缓解能力。这些结果表明,气动驱动提供了一种通用的方法来提高结构在静、动载荷下的性能。由于该策略不依赖于多材料界面或特定的细胞拓扑结构,因此可以广泛应用于优化各种晶格结构。
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引用次数: 0
NeuberNet: a neural operator solving elastic-plastic partial differential equations at V-notches from low-fidelity elastic simulations. NeuberNet:一个从低保真弹性模拟中求解v型缺口弹塑性偏微分方程的神经算子。
Pub Date : 2025-12-13 DOI: 10.1038/s44172-025-00549-5
Tommaso Grossi, Marco Beghini, Matteo Benedetti

Stress concentrations at geometric irregularities such as reentrant corners make it challenging to efficiently simulate localized plastic deformation in engineering materials. Fully nonlinear models capture these effects accurately but are computationally costly, whereas simplified elastic analyses neglect important nonlinearities. Here, we present NeuberNet, a Multi-Task Nonlinear Manifold Decoder that learns mappings between far-field displacement boundary conditions from low-fidelity elastic simulations and the corresponding high-resolution stress and strain fields derived from elastic-plastic axisymmetric solid mechanics, under assumptions of small-scale plasticity and bilinear isotropic hardening. NeuberNet serves as a data-driven implementation of the substructuring principle, designed to model complex geometries by activating plastic behavior only near stress raisers where nonlinearities arise. We provide guidelines for mesh resolution in low-fidelity simulations, demonstrate NeuberNet's ability to identify violations of the small-scale plasticity assumption, and assess its robustness to nonlinear hardening laws. We also show that NeuberNet generalizes to 3D problems with axisymmetric geometries and non-symmetric boundary conditions. Overall, NeuberNet provides a reliable and computationally efficient framework for small-scale plasticity analysis.

应力集中在几何不规则处,如可入角处,这对有效模拟工程材料的局部塑性变形具有挑战性。完全非线性模型准确地捕获了这些影响,但计算成本很高,而简化的弹性分析忽略了重要的非线性。在这里,我们提出了NeuberNet,一个多任务非线性流形解码器,它在小尺度塑性和双线性各向同性硬化的假设下,学习来自低保真弹性模拟的远场位移边界条件与来自弹塑性轴对称固体力学的相应高分辨率应力和应变场之间的映射。NeuberNet作为子结构原理的数据驱动实现,旨在通过仅在非线性应力升高处附近激活塑性行为来模拟复杂的几何形状。我们为低保真仿真中的网格分辨率提供了指导方针,展示了NeuberNet识别违反小尺度塑性假设的能力,并评估了其对非线性硬化定律的鲁棒性。我们还证明了NeuberNet可以推广到具有轴对称几何和非对称边界条件的三维问题。总的来说,NeuberNet为小规模塑性分析提供了一个可靠且计算效率高的框架。
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引用次数: 0
A flexible, magnet-based miniaturized mechanical antenna enabling low-frequency cross-medium communication between unmanned systems. 一种灵活的、基于磁性的小型化机械天线,能够在无人系统之间进行低频跨介质通信。
Pub Date : 2025-12-13 DOI: 10.1038/s44172-025-00569-1
Qingang Li, Zhi Cui, Xin Ma, Wei Yue, Ieng Hou U, Kangjie Zhou, Juntian Qu, Jianglei Chang, Yuping Huang, Chang Liu, Shuxiang Dong, Qinglei Hu, Yong Cui, Xining Zang

Extremely low frequency (ELF, 3-30 Hz) signals possess strong cross-medium communication capabilities, making them particularly well-suited for underground and underwater environments. However, traditional low-frequency (LF) transmission systems are large and inefficient, posing significant limitations in practical applications. In recent studies, mechanical antennas have been explored to generate LF signals, but current approaches rely on bulky equipment with limited range, making them unsuitable for personal use or integration into small unmanned devices. To address this challenge, this study introduces a flexible, magnet-based miniaturized LF mechanical antenna, fabricated using 3D printing. The antenna consists of a macro-fiber composite layer and a flexible permanent magnet film, and features an extremely compact volume (<6.8 cm³) and low weight (<50 g). It is also highly flexible, allowing for easy integration into diverse applications. Its transmitted signal can reach 60 m before the magnetic field strength attenuates to 1 pT. Mounted on an unmanned aerial vehicle (UAV), the antenna facilitates reliable communication between quadruped robots operating outside caves and aerial robots located deep within cave interiors, where high-frequency (HF) signals cannot penetrate. This study demonstrates robust LF cross-medium communication between UAV and ground robots in cave environments, paving the way for unmanned collaboration in scenarios inaccessible to HF wireless signals.

极低频(ELF, 3-30 Hz)信号具有很强的跨介质通信能力,特别适合于地下和水下环境。然而,传统的低频传输系统体积大,效率低,在实际应用中受到很大的限制。在最近的研究中,已经探索了机械天线来产生低频信号,但目前的方法依赖于体积庞大、范围有限的设备,这使得它们不适合个人使用或集成到小型无人设备中。为了应对这一挑战,本研究引入了一种采用3D打印制造的柔性、基于磁铁的小型化LF机械天线。该天线由巨纤维复合材料层和柔性永磁体膜组成,体积极小(
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引用次数: 0
Thermal stability enhancement of low temperature Cu-Cu bonding using metal passivation technology for advanced electronic packaging. 利用金属钝化技术增强低温Cu-Cu键合的热稳定性。
Pub Date : 2025-12-13 DOI: 10.1038/s44172-025-00551-x
Mu-Ping Hsu, Tai-Yu Lin, Hua-Jing Huang, Chiao-Yen Wang, Tsai-Fu Chung, Wen-Wei Wu, Kuan-Neng Chen

This work investigates the thermal stability of Cu-Cu bonding using a thin Ag passivation layer in applications targeting advanced packaging. Conventional Cu-Cu bonding often requires elevated temperatures (≥250 °C) that can exacerbate thermal stress and limit process flexibility, making multi-chip stacking more challenging. By introducing a 3 nm Ag passivation layer, we demonstrate reliable bonding at lower temperatures with improved durability against high-humidity and high-temperature environments, as confirmed by both Highly Accelerated Stress Tests (HAST) and burn-in measurements. In-situ transmission electron microscopy (TEM) and 4D-STEM strain mapping reveal that Ag diffusion along Cu grain boundaries not only retards abnormal grain growth but also reduces interfacial void formation at elevated temperatures. These enhancements collectively maintain a stable interface and superior mechanical strength relative to that for non-passivated Cu-Cu bonding. The results highlight the importance of metal passivation in enabling low-temperature Cu-Cu bonding technologies with robust thermal stability, providing the feasibility for next-generation advanced packaging platforms.

这项工作研究了在先进封装应用中使用薄Ag钝化层的Cu-Cu键合的热稳定性。传统的Cu-Cu键合通常需要更高的温度(≥250°C),这会加剧热应力并限制工艺灵活性,使多芯片堆叠更具挑战性。通过引入3nm Ag钝化层,我们证明了在较低温度下的可靠粘合,并提高了在高湿和高温环境下的耐久性,这一点得到了高加速应力测试(HAST)和老化测试的证实。原位透射电镜(TEM)和4D-STEM应变图显示,Ag沿Cu晶界的扩散不仅延缓了晶粒的异常生长,而且减少了高温下界面空洞的形成。与未钝化的Cu-Cu键合相比,这些增强共同保持了稳定的界面和优越的机械强度。研究结果强调了金属钝化在低温Cu-Cu键合技术中的重要性,该技术具有强大的热稳定性,为下一代先进封装平台提供了可行性。
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引用次数: 0
A computer vision and dynamic strain fusion approach for urban bridge weigh-in-motion. 城市桥梁动态称重的计算机视觉与动态应变融合方法。
Pub Date : 2025-12-12 DOI: 10.1038/s44172-025-00544-w
Yanjie Zhu, Yuchen Wang, Chenglin Gao, Wen Xiong

Dynamic weighing systems are crucial for bridge safety, yet current weighing systems are either too costly for widespread urban use or unable to track vehicles reliably in dense traffic. Here we present a video-strain inverse estimation system that combines computer vision with structural strain sensing to measure dynamic vehicle loads on bridges. The method employs a lightweight deep learning detector to recognize vehicle features, a multi-object tracking model to capture trajectories and lane positions, and a developed analytical algorithm to estimate vehicle weight from measured strains. We validated the system with field data from a heavily trafficked urban bridge. The system achieves vehicle recognition with near-baseline accuracy while using only 17% of the original model parameters and running 1.72 times faster, identifies lanes with complete accuracy with a missed detection rate of just 0.56%, and estimates total vehicle weights within 2% error. This low-cost and reliable approach advances intelligent bridge monitoring and supports digital twins of critical urban infrastructure.

动态称重系统对桥梁安全至关重要,但目前的称重系统要么成本过高,无法在城市广泛使用,要么无法在密集的交通中可靠地跟踪车辆。本文提出了一种将计算机视觉与结构应变传感相结合的视频应变反估计系统,用于测量桥梁上车辆的动态载荷。该方法采用轻量级深度学习检测器来识别车辆特征,采用多目标跟踪模型来捕获轨迹和车道位置,并开发了一种分析算法来根据测量的应变估计车辆重量。我们用一座交通繁忙的城市桥梁的现场数据验证了该系统。该系统仅使用17%的原始模型参数,以接近基线的精度实现车辆识别,运行速度提高1.72倍,完全准确地识别车道,漏检率仅为0.56%,估计车辆总重量误差在2%以内。这种低成本和可靠的方法推进了智能桥梁监控,并支持关键城市基础设施的数字孪生。
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引用次数: 0
Physics-informed displacement control for variable pattern printing with V-shaped PDMS stamps in roll-to-roll microcontact printing. 在卷对卷微接触印刷中的v形PDMS邮票可变图案印刷的物理通知位移控制。
Pub Date : 2025-12-11 DOI: 10.1038/s44172-025-00553-9
Jingyang Yan, Huarui Du, Xian Du

Roll-to-roll microcontact printing enables high-throughput production of flexible electronic devices by continuously transferring inks onto substrates via polydimethylsiloxane (PDMS) stamps. Traditional rectangular or cylindrical PDMS stamps yield uniform pattern sizes, limiting manufacturing versatility. This study introduces V-shaped PDMS stamps for variable pattern printing using a single stamp geometry. A physics-based deformation model was developed by combining finite element simulations and experiments to characterize the out-of-plane behavior of V-shaped PDMS under displacement. Leveraging this model, we implemented a neural network-based model predictive control system to precisely regulate vertical displacement and achieve desired pattern dimensions. Experimental results demonstrate that a single V-shaped PDMS stamp can reliably produce variable pattern sizes with high repeatability, improving the adaptability and process efficiency of roll-to-roll microcontact printing for flexible electronics manufacturing.

卷对卷微接触印刷通过聚二甲基硅氧烷(PDMS)印章连续地将油墨转移到基材上,从而实现柔性电子设备的高通量生产。传统的矩形或圆柱形PDMS邮票产生统一的图案尺寸,限制了制造的多功能性。本研究介绍了v形PDMS邮票的可变图案印刷使用单一的邮票几何形状。通过有限元模拟和实验相结合,建立了基于物理的变形模型来表征v形PDMS在位移作用下的面外行为。利用该模型,我们实现了基于神经网络的模型预测控制系统,以精确调节垂直位移并获得所需的模式尺寸。实验结果表明,单个v形PDMS印模可以可靠地产生可变尺寸的图案,重复性高,提高了柔性电子制造中卷对卷微接触印刷的适应性和工艺效率。
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引用次数: 0
Experimentally characterising the dynamical landscape of an active MEMS cantilever. 实验表征有源MEMS悬臂梁的动态景观。
Pub Date : 2025-12-10 DOI: 10.1038/s44172-025-00537-9
Seigan Hayashi, Chris Cameron, Stefanie Gutschmidt, Rua Murray, Bernd Krauskopf

Micro- and nano-electromechanical systems (M/NEMS) are in high demand for cutting-edge future technological solutions. Their strongly nonlinear nature is regarded as beneficial for optimising performance metrics for a wide range of engineering applications. As model-free experimentation remains limited to forward time observations, important dynamic features can be left uncharted, resulting in an incomplete dynamical landscape. Here we address this issue by employing experimental continuation - a model-free technique for constructing bifurcation diagrams by tracking steady-states and/or periodic responses directly in a physical experiment. This approach is unexplored for investigating micro-scale systems with fast timescales. Our state-of-the-art experiment investigates an active MEMS cantilever operated as a self-oscillator with a natural frequency of 100 kHz; the fastest timescales of a mechanical system probed with experimental continuation. We explore the cantilever's nonlinear responses to external, periodic excitation via a sequence of one-parameter response curves. By experimentally mapping out the MEMS cantilever's stable and unstable periodic orbits, we expose the dynamic landscape by rendering a multi-valued response surface across a range of forcing frequencies and amplitudes. An atypical, non-Duffing-like bifurcation structure is revealed.

微型和纳米机电系统(M/NEMS)对尖端的未来技术解决方案有很高的需求。它们的强非线性特性被认为有利于优化广泛工程应用的性能指标。由于无模型实验仍然局限于前向时间观测,重要的动态特征可能未被绘制,从而导致不完整的动态景观。在这里,我们通过使用实验延拓来解决这个问题,这是一种无模型技术,通过直接在物理实验中跟踪稳态和/或周期响应来构建分岔图。这种方法尚未被用于研究具有快速时间尺度的微尺度系统。我们最先进的实验研究了作为自振荡器的有源MEMS悬臂梁,其固有频率为100 kHz;用实验延拓探索机械系统的最快时间尺度。我们通过一系列单参数响应曲线来探索悬臂梁对外部周期性激励的非线性响应。通过实验绘制出MEMS悬臂梁的稳定和不稳定周期轨道,我们通过在一系列强迫频率和振幅范围内绘制多值响应面来揭示动态景观。显示非典型、非duffing样分叉结构。
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引用次数: 0
From waste to energy: a closed-loop two-phase anaerobic digestion system for sustainable dairy wastewater management. 从废物到能源:闭环两相厌氧消化系统的可持续乳品废水管理。
Pub Date : 2025-12-09 DOI: 10.1038/s44172-025-00568-2
Yuqi Gong, Yangzhao Guo, Pingping Huang, Xiaocheng Guo, Yanbiao Liu, Yifan Ren, Tangfu Xiao, Lei Li, Fengzhi Jiang, Siping Ji

The treatment of dairy wastewater (DW), characterized by high organic load and lipid/protein content, remains challenging due to the energy-intensive nature of aerobic processes and instability of anaerobic methods. This study developed a self-regulating two-phase anaerobic digestion (TPAD) system integrating an anaerobic baffled reactor (ABR) with an up-flow anaerobic sludge blanket (UASB) reactor. Sequential phase separation in the ABR enables microbial self-organization for staged lipid adsorption, protein denaturation, and hydrolysis-acidification, ensuring stable UASB input. Laboratory-scale operation achieved exceptional chemical oxygen demand (COD) removal (97.06-99.01%). Full-scale implementations across three Chinese provinces demonstrated robust performance, with COD removal of 78.13-93.46%, high methane content (83.20-83.94%), sludge reduction >75.00%, and reductions in energy consumption (64.71-85.03%) and greenhouse gas emissions (88.01-97.09%) compared to conventional systems. Microbial analysis confirmed functional spatial divergence. The TPAD system presents a regionally-proven, versatile, and scalable solution to transform DW management from a disposal cost into a biogas-generating process.

乳品废水(DW)具有高有机负荷和高脂/蛋白质含量的特点,由于好氧工艺的高能量性质和厌氧方法的不稳定性,其处理仍然具有挑战性。本研究开发了一种结合厌氧折流板反应器(ABR)和上流式厌氧污泥毯反应器(UASB)的自调节两相厌氧消化(TPAD)系统。ABR的顺序相分离使微生物能够自组织进行分阶段的脂质吸附、蛋白质变性和水解酸化,确保稳定的UASB输入。实验室规模操作取得了优异的化学需氧量(COD)去除率(97.06-99.01%)。在中国三个省份的全面实施显示出强劲的性能,与传统系统相比,COD去除率为78.13-93.46%,甲烷含量高(83.20-83.94%),污泥减量>75.00%,能耗降低(64.71-85.03%),温室气体排放降低(88.01-97.09%)。微生物分析证实了功能空间差异。TPAD系统提供了一种经过地区验证的、通用的、可扩展的解决方案,将废水管理从处理成本转变为沼气生成过程。
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引用次数: 0
Electrochemical sensors for in situ monitoring of reactive species during cold atmospheric plasma-based therapies. 用于低温大气等离子体治疗过程中反应物质现场监测的电化学传感器。
Pub Date : 2025-12-09 DOI: 10.1038/s44172-025-00560-w
Jonathan E Thomas, Suneel Kumar, Gagana Karkada, Julia Sutter, Kristina Pattison, Jason Rainone, Dhruv Patel, Shashank Madhavan, Fred C Krebs, Francois Berthiaume, Vandana Miller, Katharina Stapelmann

Cold atmospheric pressure plasma (CAP) is emerging as a clinically relevant therapy for dermatological conditions such as actinic keratosis, warts, and chronic wounds. However, these therapies lack strategies to monitor CAP delivery in situ and to ensure delivery of an effective CAP dose without unwanted toxicity. CAP acts as a therapeutic agent in these biomedical applications primarily (but not solely) through reactive oxygen and nitrogen species (RONS) generated at transiently high local concentrations. Here we demonstrate the use of bio-electrochemical sensors capable of real-time measurements of key CAP RONS: hydrogen peroxide and oxidation-reduction-potential (ORP). In in vitro scratch assays and in vivo murine wound models, we used these sensors to establish dose-response relationships that link CAP exposure with wound (scratch) closure dynamics, cell proliferation, oxidative stress response, and scar reduction. Our results demonstrate that CAP treatment can be continuously monitored and actively controlled in situ, providing a framework for precision plasma medicine and safer, more effective clinical translation of CAP.

冷大气压等离子体(CAP)正在成为一种临床相关的皮肤病治疗方法,如光化性角化病、疣和慢性伤口。然而,这些疗法缺乏监测原位CAP递送的策略,并确保有效CAP剂量的递送没有不必要的毒性。在这些生物医学应用中,CAP主要(但不仅是)通过局部瞬时高浓度产生的活性氧和活性氮(RONS)发挥治疗剂的作用。在这里,我们展示了能够实时测量关键CAP RONS的生物电化学传感器的使用:过氧化氢和氧化还原电位(ORP)。在体外划伤试验和体内小鼠伤口模型中,我们使用这些传感器建立了CAP暴露与伤口(划伤)闭合动力学、细胞增殖、氧化应激反应和疤痕减少之间的剂量-反应关系。我们的研究结果表明,CAP治疗可以持续监测和主动就地控制,为精准血浆医学和更安全、更有效的CAP临床翻译提供了框架。
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引用次数: 0
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Communications engineering
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