Pub Date : 2025-12-15DOI: 10.1038/s44172-025-00566-4
Hao Qiu, Matthew C Tang, Selene K Roberts, Guoli Li, Rong Su, Marisa L Martin-Fernandez, David T Clarke, Shugang Liu, Xiaojie Liu, Lin Wang
Single-molecule localization microscopy achieves nanometer-scale resolution but is compromised by sample drift during image acquisition. Here we present reinforced optical cage systems, a novel approach that eliminates drift at its mechanical source rather than correcting it through complex image post-processing or fiducial markers. Reinforced optical cage systems employ perforated optomechanical components interconnected by tungsten-steel rods in a design proven by mechanical stability simulations. Our bench-top microscope, built with reinforced optical cage systems, demonstrated exceptional three-dimensional stability, with mean cumulative lateral drift of approximately 5 nanometers over 2 h in widefield fluorescence microscopy and 11-16 nanometers over 15 min in single-molecule localization microscopy, free from measurable axial drift. This development allows super-resolution microscopy to reach its full resolution without the necessity of sample drift correction, offering a straightforward, cost-effective, low-maintenance, and readily accessible solution to high-performance super-resolution microscopy. By addressing the fundamental issue of mechanical instability, reinforced optical cage systems enable improved precision instrumentation for the broader scientific and engineering community.
{"title":"Reinforced optical cage systems enable drift-free single-molecule localization microscopy.","authors":"Hao Qiu, Matthew C Tang, Selene K Roberts, Guoli Li, Rong Su, Marisa L Martin-Fernandez, David T Clarke, Shugang Liu, Xiaojie Liu, Lin Wang","doi":"10.1038/s44172-025-00566-4","DOIUrl":"10.1038/s44172-025-00566-4","url":null,"abstract":"<p><p>Single-molecule localization microscopy achieves nanometer-scale resolution but is compromised by sample drift during image acquisition. Here we present reinforced optical cage systems, a novel approach that eliminates drift at its mechanical source rather than correcting it through complex image post-processing or fiducial markers. Reinforced optical cage systems employ perforated optomechanical components interconnected by tungsten-steel rods in a design proven by mechanical stability simulations. Our bench-top microscope, built with reinforced optical cage systems, demonstrated exceptional three-dimensional stability, with mean cumulative lateral drift of approximately 5 nanometers over 2 h in widefield fluorescence microscopy and 11-16 nanometers over 15 min in single-molecule localization microscopy, free from measurable axial drift. This development allows super-resolution microscopy to reach its full resolution without the necessity of sample drift correction, offering a straightforward, cost-effective, low-maintenance, and readily accessible solution to high-performance super-resolution microscopy. By addressing the fundamental issue of mechanical instability, reinforced optical cage systems enable improved precision instrumentation for the broader scientific and engineering community.</p>","PeriodicalId":72644,"journal":{"name":"Communications engineering","volume":" ","pages":"13"},"PeriodicalIF":0.0,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12820167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145764717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-13DOI: 10.1038/s44172-025-00570-8
Xiaoheng Zhu, Yucong Hua, Dengge Jin, Jordan R Raney
Buckling is a common failure mode in lattice structures, limiting their use in some applications. The tendency of a strut to buckle is related to the local nodal connectivity. In this work, we introduce a pneumatic actuation strategy to actively tune the mechanical behavior of lattice structures by locally reconfiguring their effective nodal connectivity. By selectively inflating pneumatic actuators embedded in the lattice into spatial patterns with varying levels of connectivity, we demonstrate a method to modulate mechanical properties, including stiffness and buckling response. The most reinforced pattern can lead to 121.6% improvement in buckling strength relative to the regular lattice itself. Additionally, the post-buckling behavior of pneumatically controlled lattices can be programmably tuned by varying the input air pressure signals. The pneumatically controlled lattices reduced the peak acceleration by 50.9%, demonstrating enhanced impact mitigation capability. These results show that pneumatic actuation provides a versatile approach to enhancing structural performance under both static and dynamic loading. Since this strategy does not rely on multi-material interfaces or specific cell topologies, it can be broadly applied to optimize a wide range of lattice architectures.
{"title":"Pneumatically controlled lattices with tunable mechanical behavior.","authors":"Xiaoheng Zhu, Yucong Hua, Dengge Jin, Jordan R Raney","doi":"10.1038/s44172-025-00570-8","DOIUrl":"10.1038/s44172-025-00570-8","url":null,"abstract":"<p><p>Buckling is a common failure mode in lattice structures, limiting their use in some applications. The tendency of a strut to buckle is related to the local nodal connectivity. In this work, we introduce a pneumatic actuation strategy to actively tune the mechanical behavior of lattice structures by locally reconfiguring their effective nodal connectivity. By selectively inflating pneumatic actuators embedded in the lattice into spatial patterns with varying levels of connectivity, we demonstrate a method to modulate mechanical properties, including stiffness and buckling response. The most reinforced pattern can lead to 121.6% improvement in buckling strength relative to the regular lattice itself. Additionally, the post-buckling behavior of pneumatically controlled lattices can be programmably tuned by varying the input air pressure signals. The pneumatically controlled lattices reduced the peak acceleration by 50.9%, demonstrating enhanced impact mitigation capability. These results show that pneumatic actuation provides a versatile approach to enhancing structural performance under both static and dynamic loading. Since this strategy does not rely on multi-material interfaces or specific cell topologies, it can be broadly applied to optimize a wide range of lattice architectures.</p>","PeriodicalId":72644,"journal":{"name":"Communications engineering","volume":" ","pages":"14"},"PeriodicalIF":0.0,"publicationDate":"2025-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12820119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145752336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-13DOI: 10.1038/s44172-025-00549-5
Tommaso Grossi, Marco Beghini, Matteo Benedetti
Stress concentrations at geometric irregularities such as reentrant corners make it challenging to efficiently simulate localized plastic deformation in engineering materials. Fully nonlinear models capture these effects accurately but are computationally costly, whereas simplified elastic analyses neglect important nonlinearities. Here, we present NeuberNet, a Multi-Task Nonlinear Manifold Decoder that learns mappings between far-field displacement boundary conditions from low-fidelity elastic simulations and the corresponding high-resolution stress and strain fields derived from elastic-plastic axisymmetric solid mechanics, under assumptions of small-scale plasticity and bilinear isotropic hardening. NeuberNet serves as a data-driven implementation of the substructuring principle, designed to model complex geometries by activating plastic behavior only near stress raisers where nonlinearities arise. We provide guidelines for mesh resolution in low-fidelity simulations, demonstrate NeuberNet's ability to identify violations of the small-scale plasticity assumption, and assess its robustness to nonlinear hardening laws. We also show that NeuberNet generalizes to 3D problems with axisymmetric geometries and non-symmetric boundary conditions. Overall, NeuberNet provides a reliable and computationally efficient framework for small-scale plasticity analysis.
{"title":"NeuberNet: a neural operator solving elastic-plastic partial differential equations at V-notches from low-fidelity elastic simulations.","authors":"Tommaso Grossi, Marco Beghini, Matteo Benedetti","doi":"10.1038/s44172-025-00549-5","DOIUrl":"10.1038/s44172-025-00549-5","url":null,"abstract":"<p><p>Stress concentrations at geometric irregularities such as reentrant corners make it challenging to efficiently simulate localized plastic deformation in engineering materials. Fully nonlinear models capture these effects accurately but are computationally costly, whereas simplified elastic analyses neglect important nonlinearities. Here, we present NeuberNet, a Multi-Task Nonlinear Manifold Decoder that learns mappings between far-field displacement boundary conditions from low-fidelity elastic simulations and the corresponding high-resolution stress and strain fields derived from elastic-plastic axisymmetric solid mechanics, under assumptions of small-scale plasticity and bilinear isotropic hardening. NeuberNet serves as a data-driven implementation of the substructuring principle, designed to model complex geometries by activating plastic behavior only near stress raisers where nonlinearities arise. We provide guidelines for mesh resolution in low-fidelity simulations, demonstrate NeuberNet's ability to identify violations of the small-scale plasticity assumption, and assess its robustness to nonlinear hardening laws. We also show that NeuberNet generalizes to 3D problems with axisymmetric geometries and non-symmetric boundary conditions. Overall, NeuberNet provides a reliable and computationally efficient framework for small-scale plasticity analysis.</p>","PeriodicalId":72644,"journal":{"name":"Communications engineering","volume":" ","pages":"218"},"PeriodicalIF":0.0,"publicationDate":"2025-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145752302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Extremely low frequency (ELF, 3-30 Hz) signals possess strong cross-medium communication capabilities, making them particularly well-suited for underground and underwater environments. However, traditional low-frequency (LF) transmission systems are large and inefficient, posing significant limitations in practical applications. In recent studies, mechanical antennas have been explored to generate LF signals, but current approaches rely on bulky equipment with limited range, making them unsuitable for personal use or integration into small unmanned devices. To address this challenge, this study introduces a flexible, magnet-based miniaturized LF mechanical antenna, fabricated using 3D printing. The antenna consists of a macro-fiber composite layer and a flexible permanent magnet film, and features an extremely compact volume (<6.8 cm³) and low weight (<50 g). It is also highly flexible, allowing for easy integration into diverse applications. Its transmitted signal can reach 60 m before the magnetic field strength attenuates to 1 pT. Mounted on an unmanned aerial vehicle (UAV), the antenna facilitates reliable communication between quadruped robots operating outside caves and aerial robots located deep within cave interiors, where high-frequency (HF) signals cannot penetrate. This study demonstrates robust LF cross-medium communication between UAV and ground robots in cave environments, paving the way for unmanned collaboration in scenarios inaccessible to HF wireless signals.
{"title":"A flexible, magnet-based miniaturized mechanical antenna enabling low-frequency cross-medium communication between unmanned systems.","authors":"Qingang Li, Zhi Cui, Xin Ma, Wei Yue, Ieng Hou U, Kangjie Zhou, Juntian Qu, Jianglei Chang, Yuping Huang, Chang Liu, Shuxiang Dong, Qinglei Hu, Yong Cui, Xining Zang","doi":"10.1038/s44172-025-00569-1","DOIUrl":"10.1038/s44172-025-00569-1","url":null,"abstract":"<p><p>Extremely low frequency (ELF, 3-30 Hz) signals possess strong cross-medium communication capabilities, making them particularly well-suited for underground and underwater environments. However, traditional low-frequency (LF) transmission systems are large and inefficient, posing significant limitations in practical applications. In recent studies, mechanical antennas have been explored to generate LF signals, but current approaches rely on bulky equipment with limited range, making them unsuitable for personal use or integration into small unmanned devices. To address this challenge, this study introduces a flexible, magnet-based miniaturized LF mechanical antenna, fabricated using 3D printing. The antenna consists of a macro-fiber composite layer and a flexible permanent magnet film, and features an extremely compact volume (<6.8 cm³) and low weight (<50 g). It is also highly flexible, allowing for easy integration into diverse applications. Its transmitted signal can reach 60 m before the magnetic field strength attenuates to 1 pT. Mounted on an unmanned aerial vehicle (UAV), the antenna facilitates reliable communication between quadruped robots operating outside caves and aerial robots located deep within cave interiors, where high-frequency (HF) signals cannot penetrate. This study demonstrates robust LF cross-medium communication between UAV and ground robots in cave environments, paving the way for unmanned collaboration in scenarios inaccessible to HF wireless signals.</p>","PeriodicalId":72644,"journal":{"name":"Communications engineering","volume":" ","pages":"15"},"PeriodicalIF":0.0,"publicationDate":"2025-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12820373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145752357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This work investigates the thermal stability of Cu-Cu bonding using a thin Ag passivation layer in applications targeting advanced packaging. Conventional Cu-Cu bonding often requires elevated temperatures (≥250 °C) that can exacerbate thermal stress and limit process flexibility, making multi-chip stacking more challenging. By introducing a 3 nm Ag passivation layer, we demonstrate reliable bonding at lower temperatures with improved durability against high-humidity and high-temperature environments, as confirmed by both Highly Accelerated Stress Tests (HAST) and burn-in measurements. In-situ transmission electron microscopy (TEM) and 4D-STEM strain mapping reveal that Ag diffusion along Cu grain boundaries not only retards abnormal grain growth but also reduces interfacial void formation at elevated temperatures. These enhancements collectively maintain a stable interface and superior mechanical strength relative to that for non-passivated Cu-Cu bonding. The results highlight the importance of metal passivation in enabling low-temperature Cu-Cu bonding technologies with robust thermal stability, providing the feasibility for next-generation advanced packaging platforms.
{"title":"Thermal stability enhancement of low temperature Cu-Cu bonding using metal passivation technology for advanced electronic packaging.","authors":"Mu-Ping Hsu, Tai-Yu Lin, Hua-Jing Huang, Chiao-Yen Wang, Tsai-Fu Chung, Wen-Wei Wu, Kuan-Neng Chen","doi":"10.1038/s44172-025-00551-x","DOIUrl":"10.1038/s44172-025-00551-x","url":null,"abstract":"<p><p>This work investigates the thermal stability of Cu-Cu bonding using a thin Ag passivation layer in applications targeting advanced packaging. Conventional Cu-Cu bonding often requires elevated temperatures (≥250 °C) that can exacerbate thermal stress and limit process flexibility, making multi-chip stacking more challenging. By introducing a 3 nm Ag passivation layer, we demonstrate reliable bonding at lower temperatures with improved durability against high-humidity and high-temperature environments, as confirmed by both Highly Accelerated Stress Tests (HAST) and burn-in measurements. In-situ transmission electron microscopy (TEM) and 4D-STEM strain mapping reveal that Ag diffusion along Cu grain boundaries not only retards abnormal grain growth but also reduces interfacial void formation at elevated temperatures. These enhancements collectively maintain a stable interface and superior mechanical strength relative to that for non-passivated Cu-Cu bonding. The results highlight the importance of metal passivation in enabling low-temperature Cu-Cu bonding technologies with robust thermal stability, providing the feasibility for next-generation advanced packaging platforms.</p>","PeriodicalId":72644,"journal":{"name":"Communications engineering","volume":" ","pages":"216"},"PeriodicalIF":0.0,"publicationDate":"2025-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12728164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145752313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-12DOI: 10.1038/s44172-025-00544-w
Yanjie Zhu, Yuchen Wang, Chenglin Gao, Wen Xiong
Dynamic weighing systems are crucial for bridge safety, yet current weighing systems are either too costly for widespread urban use or unable to track vehicles reliably in dense traffic. Here we present a video-strain inverse estimation system that combines computer vision with structural strain sensing to measure dynamic vehicle loads on bridges. The method employs a lightweight deep learning detector to recognize vehicle features, a multi-object tracking model to capture trajectories and lane positions, and a developed analytical algorithm to estimate vehicle weight from measured strains. We validated the system with field data from a heavily trafficked urban bridge. The system achieves vehicle recognition with near-baseline accuracy while using only 17% of the original model parameters and running 1.72 times faster, identifies lanes with complete accuracy with a missed detection rate of just 0.56%, and estimates total vehicle weights within 2% error. This low-cost and reliable approach advances intelligent bridge monitoring and supports digital twins of critical urban infrastructure.
{"title":"A computer vision and dynamic strain fusion approach for urban bridge weigh-in-motion.","authors":"Yanjie Zhu, Yuchen Wang, Chenglin Gao, Wen Xiong","doi":"10.1038/s44172-025-00544-w","DOIUrl":"10.1038/s44172-025-00544-w","url":null,"abstract":"<p><p>Dynamic weighing systems are crucial for bridge safety, yet current weighing systems are either too costly for widespread urban use or unable to track vehicles reliably in dense traffic. Here we present a video-strain inverse estimation system that combines computer vision with structural strain sensing to measure dynamic vehicle loads on bridges. The method employs a lightweight deep learning detector to recognize vehicle features, a multi-object tracking model to capture trajectories and lane positions, and a developed analytical algorithm to estimate vehicle weight from measured strains. We validated the system with field data from a heavily trafficked urban bridge. The system achieves vehicle recognition with near-baseline accuracy while using only 17% of the original model parameters and running 1.72 times faster, identifies lanes with complete accuracy with a missed detection rate of just 0.56%, and estimates total vehicle weights within 2% error. This low-cost and reliable approach advances intelligent bridge monitoring and supports digital twins of critical urban infrastructure.</p>","PeriodicalId":72644,"journal":{"name":"Communications engineering","volume":" ","pages":"212"},"PeriodicalIF":0.0,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12717240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145745854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-11DOI: 10.1038/s44172-025-00553-9
Jingyang Yan, Huarui Du, Xian Du
Roll-to-roll microcontact printing enables high-throughput production of flexible electronic devices by continuously transferring inks onto substrates via polydimethylsiloxane (PDMS) stamps. Traditional rectangular or cylindrical PDMS stamps yield uniform pattern sizes, limiting manufacturing versatility. This study introduces V-shaped PDMS stamps for variable pattern printing using a single stamp geometry. A physics-based deformation model was developed by combining finite element simulations and experiments to characterize the out-of-plane behavior of V-shaped PDMS under displacement. Leveraging this model, we implemented a neural network-based model predictive control system to precisely regulate vertical displacement and achieve desired pattern dimensions. Experimental results demonstrate that a single V-shaped PDMS stamp can reliably produce variable pattern sizes with high repeatability, improving the adaptability and process efficiency of roll-to-roll microcontact printing for flexible electronics manufacturing.
{"title":"Physics-informed displacement control for variable pattern printing with V-shaped PDMS stamps in roll-to-roll microcontact printing.","authors":"Jingyang Yan, Huarui Du, Xian Du","doi":"10.1038/s44172-025-00553-9","DOIUrl":"10.1038/s44172-025-00553-9","url":null,"abstract":"<p><p>Roll-to-roll microcontact printing enables high-throughput production of flexible electronic devices by continuously transferring inks onto substrates via polydimethylsiloxane (PDMS) stamps. Traditional rectangular or cylindrical PDMS stamps yield uniform pattern sizes, limiting manufacturing versatility. This study introduces V-shaped PDMS stamps for variable pattern printing using a single stamp geometry. A physics-based deformation model was developed by combining finite element simulations and experiments to characterize the out-of-plane behavior of V-shaped PDMS under displacement. Leveraging this model, we implemented a neural network-based model predictive control system to precisely regulate vertical displacement and achieve desired pattern dimensions. Experimental results demonstrate that a single V-shaped PDMS stamp can reliably produce variable pattern sizes with high repeatability, improving the adaptability and process efficiency of roll-to-roll microcontact printing for flexible electronics manufacturing.</p>","PeriodicalId":72644,"journal":{"name":"Communications engineering","volume":" ","pages":"214"},"PeriodicalIF":0.0,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145745907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-10DOI: 10.1038/s44172-025-00537-9
Seigan Hayashi, Chris Cameron, Stefanie Gutschmidt, Rua Murray, Bernd Krauskopf
Micro- and nano-electromechanical systems (M/NEMS) are in high demand for cutting-edge future technological solutions. Their strongly nonlinear nature is regarded as beneficial for optimising performance metrics for a wide range of engineering applications. As model-free experimentation remains limited to forward time observations, important dynamic features can be left uncharted, resulting in an incomplete dynamical landscape. Here we address this issue by employing experimental continuation - a model-free technique for constructing bifurcation diagrams by tracking steady-states and/or periodic responses directly in a physical experiment. This approach is unexplored for investigating micro-scale systems with fast timescales. Our state-of-the-art experiment investigates an active MEMS cantilever operated as a self-oscillator with a natural frequency of 100 kHz; the fastest timescales of a mechanical system probed with experimental continuation. We explore the cantilever's nonlinear responses to external, periodic excitation via a sequence of one-parameter response curves. By experimentally mapping out the MEMS cantilever's stable and unstable periodic orbits, we expose the dynamic landscape by rendering a multi-valued response surface across a range of forcing frequencies and amplitudes. An atypical, non-Duffing-like bifurcation structure is revealed.
{"title":"Experimentally characterising the dynamical landscape of an active MEMS cantilever.","authors":"Seigan Hayashi, Chris Cameron, Stefanie Gutschmidt, Rua Murray, Bernd Krauskopf","doi":"10.1038/s44172-025-00537-9","DOIUrl":"10.1038/s44172-025-00537-9","url":null,"abstract":"<p><p>Micro- and nano-electromechanical systems (M/NEMS) are in high demand for cutting-edge future technological solutions. Their strongly nonlinear nature is regarded as beneficial for optimising performance metrics for a wide range of engineering applications. As model-free experimentation remains limited to forward time observations, important dynamic features can be left uncharted, resulting in an incomplete dynamical landscape. Here we address this issue by employing experimental continuation - a model-free technique for constructing bifurcation diagrams by tracking steady-states and/or periodic responses directly in a physical experiment. This approach is unexplored for investigating micro-scale systems with fast timescales. Our state-of-the-art experiment investigates an active MEMS cantilever operated as a self-oscillator with a natural frequency of 100 kHz; the fastest timescales of a mechanical system probed with experimental continuation. We explore the cantilever's nonlinear responses to external, periodic excitation via a sequence of one-parameter response curves. By experimentally mapping out the MEMS cantilever's stable and unstable periodic orbits, we expose the dynamic landscape by rendering a multi-valued response surface across a range of forcing frequencies and amplitudes. An atypical, non-Duffing-like bifurcation structure is revealed.</p>","PeriodicalId":72644,"journal":{"name":"Communications engineering","volume":"4 1","pages":"211"},"PeriodicalIF":0.0,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12695876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145727395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-09DOI: 10.1038/s44172-025-00568-2
Yuqi Gong, Yangzhao Guo, Pingping Huang, Xiaocheng Guo, Yanbiao Liu, Yifan Ren, Tangfu Xiao, Lei Li, Fengzhi Jiang, Siping Ji
The treatment of dairy wastewater (DW), characterized by high organic load and lipid/protein content, remains challenging due to the energy-intensive nature of aerobic processes and instability of anaerobic methods. This study developed a self-regulating two-phase anaerobic digestion (TPAD) system integrating an anaerobic baffled reactor (ABR) with an up-flow anaerobic sludge blanket (UASB) reactor. Sequential phase separation in the ABR enables microbial self-organization for staged lipid adsorption, protein denaturation, and hydrolysis-acidification, ensuring stable UASB input. Laboratory-scale operation achieved exceptional chemical oxygen demand (COD) removal (97.06-99.01%). Full-scale implementations across three Chinese provinces demonstrated robust performance, with COD removal of 78.13-93.46%, high methane content (83.20-83.94%), sludge reduction >75.00%, and reductions in energy consumption (64.71-85.03%) and greenhouse gas emissions (88.01-97.09%) compared to conventional systems. Microbial analysis confirmed functional spatial divergence. The TPAD system presents a regionally-proven, versatile, and scalable solution to transform DW management from a disposal cost into a biogas-generating process.
{"title":"From waste to energy: a closed-loop two-phase anaerobic digestion system for sustainable dairy wastewater management.","authors":"Yuqi Gong, Yangzhao Guo, Pingping Huang, Xiaocheng Guo, Yanbiao Liu, Yifan Ren, Tangfu Xiao, Lei Li, Fengzhi Jiang, Siping Ji","doi":"10.1038/s44172-025-00568-2","DOIUrl":"10.1038/s44172-025-00568-2","url":null,"abstract":"<p><p>The treatment of dairy wastewater (DW), characterized by high organic load and lipid/protein content, remains challenging due to the energy-intensive nature of aerobic processes and instability of anaerobic methods. This study developed a self-regulating two-phase anaerobic digestion (TPAD) system integrating an anaerobic baffled reactor (ABR) with an up-flow anaerobic sludge blanket (UASB) reactor. Sequential phase separation in the ABR enables microbial self-organization for staged lipid adsorption, protein denaturation, and hydrolysis-acidification, ensuring stable UASB input. Laboratory-scale operation achieved exceptional chemical oxygen demand (COD) removal (97.06-99.01%). Full-scale implementations across three Chinese provinces demonstrated robust performance, with COD removal of 78.13-93.46%, high methane content (83.20-83.94%), sludge reduction >75.00%, and reductions in energy consumption (64.71-85.03%) and greenhouse gas emissions (88.01-97.09%) compared to conventional systems. Microbial analysis confirmed functional spatial divergence. The TPAD system presents a regionally-proven, versatile, and scalable solution to transform DW management from a disposal cost into a biogas-generating process.</p>","PeriodicalId":72644,"journal":{"name":"Communications engineering","volume":" ","pages":"12"},"PeriodicalIF":0.0,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12820218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145716823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-09DOI: 10.1038/s44172-025-00560-w
Jonathan E Thomas, Suneel Kumar, Gagana Karkada, Julia Sutter, Kristina Pattison, Jason Rainone, Dhruv Patel, Shashank Madhavan, Fred C Krebs, Francois Berthiaume, Vandana Miller, Katharina Stapelmann
Cold atmospheric pressure plasma (CAP) is emerging as a clinically relevant therapy for dermatological conditions such as actinic keratosis, warts, and chronic wounds. However, these therapies lack strategies to monitor CAP delivery in situ and to ensure delivery of an effective CAP dose without unwanted toxicity. CAP acts as a therapeutic agent in these biomedical applications primarily (but not solely) through reactive oxygen and nitrogen species (RONS) generated at transiently high local concentrations. Here we demonstrate the use of bio-electrochemical sensors capable of real-time measurements of key CAP RONS: hydrogen peroxide and oxidation-reduction-potential (ORP). In in vitro scratch assays and in vivo murine wound models, we used these sensors to establish dose-response relationships that link CAP exposure with wound (scratch) closure dynamics, cell proliferation, oxidative stress response, and scar reduction. Our results demonstrate that CAP treatment can be continuously monitored and actively controlled in situ, providing a framework for precision plasma medicine and safer, more effective clinical translation of CAP.
{"title":"Electrochemical sensors for in situ monitoring of reactive species during cold atmospheric plasma-based therapies.","authors":"Jonathan E Thomas, Suneel Kumar, Gagana Karkada, Julia Sutter, Kristina Pattison, Jason Rainone, Dhruv Patel, Shashank Madhavan, Fred C Krebs, Francois Berthiaume, Vandana Miller, Katharina Stapelmann","doi":"10.1038/s44172-025-00560-w","DOIUrl":"10.1038/s44172-025-00560-w","url":null,"abstract":"<p><p>Cold atmospheric pressure plasma (CAP) is emerging as a clinically relevant therapy for dermatological conditions such as actinic keratosis, warts, and chronic wounds. However, these therapies lack strategies to monitor CAP delivery in situ and to ensure delivery of an effective CAP dose without unwanted toxicity. CAP acts as a therapeutic agent in these biomedical applications primarily (but not solely) through reactive oxygen and nitrogen species (RONS) generated at transiently high local concentrations. Here we demonstrate the use of bio-electrochemical sensors capable of real-time measurements of key CAP RONS: hydrogen peroxide and oxidation-reduction-potential (ORP). In in vitro scratch assays and in vivo murine wound models, we used these sensors to establish dose-response relationships that link CAP exposure with wound (scratch) closure dynamics, cell proliferation, oxidative stress response, and scar reduction. Our results demonstrate that CAP treatment can be continuously monitored and actively controlled in situ, providing a framework for precision plasma medicine and safer, more effective clinical translation of CAP.</p>","PeriodicalId":72644,"journal":{"name":"Communications engineering","volume":" ","pages":"6"},"PeriodicalIF":0.0,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12770570/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145709485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}