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Physicochemical characterization and antidiabetic potential of the food formulation from Momordica balsamina leaf and Vigna subterranea seed in dexamethasone-induced insulin resistance rats 地塞米松诱导胰岛素抵抗大鼠的苦瓜叶和苦瓜籽食品配方理化特性及降糖作用
Pub Date : 2025-11-22 DOI: 10.1016/j.focha.2025.101173
Daniel Maiwé , Aboubakar , David Miaffo
Momordica balsamina is a plant widely distributed in Africa and is used in traditional medicine to treat diabetes. Vigna subterranea is a legume, widely cultivated in sub-Saharan Africa and used for its nutritional and therapeutic properties. The present study aims to evaluate the effects of the food formulations from M. balsamina leaves and V. subterranea seeds (FF) in insulin-resistant rats. Insulin resistance was induced in male rats by subcutaneous injection of dexamethasone (1 mg/kg) one hour after pretreatment with metformin (Glucophage) and FF1: 40/60(%), FF2: 60/40(%), and FF3: 50/50(%). Body weight, glycemia, insulinemia, food intake, water consumption, glycogen, hepatic enzymes, lipid and oxidative profile, and hepatorenal markers were assessed. FF3 and metformin (Glucophage) significantly increased body weight and decreased food and water intake. Anti-atherogenic and HOMA-IR indices, ALT, AST, glucose-6-phosphatase and glucose-6-phosphate dehydrogenase activities, and glucose, insulin, cholesterol, LDL-c, triglycerides, nitric oxide, and malondialdehyde levels were significantly reduced in insulin-resistant rats fed with various FF. There was also a significant increase in glucokinase, CAT and SOD activities, as well as glycogen, HDL-c, reduced glutathione, creatinine, urea, and uric acid levels. FF exerts an antidiabetic effect by reducing hyperglycemia and hyperlipidemia, and by protecting the kidneys, liver, and heart from injury.
苦瓜(Momordica balsamina)是一种广泛分布在非洲的植物,在传统医学中用于治疗糖尿病。地下维格纳是一种豆科植物,在撒哈拉以南非洲广泛种植,并因其营养和治疗特性而被使用。本研究旨在评价香草叶和地下香草籽(FF)食物配方对胰岛素抵抗大鼠的影响。二甲双胍(Glucophage)与FF1∶40/60(%)、FF2∶60/40(%)、FF3∶50/50(%)预处理1 h后皮下注射地塞米松(1 mg/kg)诱导雄性大鼠胰岛素抵抗。评估体重、血糖、胰岛素血症、食物摄入、水消耗、糖原、肝酶、脂质和氧化谱以及肝肾标志物。FF3和二甲双胍(Glucophage)显著增加体重,减少食物和水的摄入量。饲喂不同种类FF后,胰岛素抵抗大鼠的抗动脉粥样硬化和HOMA-IR指标、ALT、AST、葡萄糖-6-磷酸酶和葡萄糖-6-磷酸脱氢酶活性以及葡萄糖、胰岛素、胆固醇、LDL-c、甘油三酯、一氧化氮和丙二醛水平均显著降低。葡萄糖激酶、CAT和SOD活性以及糖原、HDL-c、还原性谷胱甘肽、肌酐、尿素和尿酸水平也显著增加。FF通过降低高血糖和高脂血症,保护肾脏、肝脏和心脏免受损伤,发挥抗糖尿病作用。
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引用次数: 0
High pressure processing of sweet corn milk yogurt: A plant-based probiotic carrier with enhanced stability 玉米甜乳酸奶高压加工:一种稳定性增强的植物性益生菌载体
Pub Date : 2025-11-21 DOI: 10.1016/j.focha.2025.101174
Pittaya Chaikham , Suttipong Yutsapremanon , Sasitorn Baipong , Young Hoon Jung , Wanli Zhang , Sarisa Suriyarak , Saroat Rawdkuen , Samart Sai-Ut , Sani Jirasatid
Sweet corn milk yogurt represents a promising plant-based matrix for probiotic delivery, but maintaining microbial viability and product quality remains a challenge. High-pressure processing (HPP) offers a non-thermal approach to improve stability. This study examined the effects of HPP at 100–300 MPa for 10 min on probiotic survival, physicochemical quality, and storage stability of sweet corn milk yogurt fortified with Lacticaseibacillus casei 01, Streptococcus thermophilus, and Lactobacillus delbrueckii subsp. bulgaricus. Probiotic counts were monitored during simulated gastrointestinal (GI) digestion and 6 weeks of refrigerated storage (4 °C). All strains declined significantly at ≥200 MPa, with the greatest reduction at 300 MPa (p ≤ 0.05). Among the tested microbes, L. casei 01 exhibited higher tolerance to GI conditions, although survival decreased with increasing pressure. Yogurt processed at 100 MPa maintained viable probiotic levels above 7 log CFU/g, with only slight changes in pH, titratable acidity, sugar content, and browning index. Moderate syneresis and viscosity reduction were also observed. In contrast, higher pressures negatively affected microbial viability and texture. Overall, HPP at 100 MPa was most effective in preserving probiotic viability and yogurt quality, supporting its application as a viable method to extend shelf life and enhance functionality of plant-based probiotic products.
甜玉米乳酸奶代表了一种很有前途的植物基益生菌基质,但保持微生物活力和产品质量仍然是一个挑战。高压处理(HPP)提供了一种非热的方法来提高稳定性。本研究考察了HPP在100-300 MPa下发酵10 min对添加干酪乳杆菌01、嗜热链球菌和德尔布鲁氏乳杆菌亚种的甜玉米乳酸奶益生菌存活、理化品质和储存稳定性的影响。发酵剂。保加利亚在模拟胃肠道(GI)消化和6周冷藏(4°C)期间监测益生菌计数。所有菌株在≥200 MPa时均显著下降,其中300 MPa时下降幅度最大(p≤0.05)。在所测试的微生物中,干酪乳杆菌01对胃肠道条件表现出更高的耐受性,尽管存活率随着压力的增加而下降。在100 MPa下加工的酸奶中,益生菌的活性水平维持在7 log CFU/g以上,pH、可滴定酸度、糖含量和褐变指数仅发生轻微变化。还观察到适度的增效作用和粘度降低。相反,较高的压力对微生物活力和结构产生负面影响。总体而言,100 MPa下的HPP在保持益生菌活力和酸奶质量方面最有效,支持其作为延长植物性益生菌产品保质期和增强功能的可行方法的应用。
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引用次数: 0
Relative modulatory potential of quercetin, catechin, silymarin and genistein on cellular antioxidant system and liver histology during gentamicin-induced acute toxicity in rats 槲皮素、儿茶素、水飞蓟素和染料木素对庆大霉素急性毒性大鼠细胞抗氧化系统和肝脏组织的相对调节作用
Pub Date : 2025-11-19 DOI: 10.1016/j.focha.2025.101169
Pawan K Verma , Bhawani Singh , Priyanka Sharma , Sheen Tukra , Shilpa Sood , Biswajit Brahma , Gholamreza Abdi , Zuhaib F. Bhat
The study aimed to determine the modulatory potential of different phytochemicals (quercetin, catechin, silymarin and genistein) on the hepatic antioxidant system and histology during acute toxicity induced by gentamicin (GEN) in rats. Adult Wistar rats (n = 66) were randomly allocated to eleven groups. Administration of GEN increased (p < 0.05) plasma activities of AST, ALT, GGT and ALP in a dose-dependent manner, indicating acute hepatic dysfunctions in rats. It also increased (p < 0.05) levels of malondialdehyde (MDA) and advanced oxidation protein product (AOPP) in hepatic tissues and reduced total antioxidant status (TAS), total thiols (TTH), reduced glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) activities, indicating intensity of hepatic damage. These observations accompanied various histopathological changes such as infiltration of inflammatory cells in the vicinity of severely atrophied hepatocytes, degenerative changes, vacuolar degeneration or complete loss of parenchyma in the liver in a dose-dependent manner. The concurrent exposure of phytochemicals along with GEN significantly (p < 0.05) reduced hepatic tissue damage as indicated by reduced levels of MDA and AOPP in hepatic tissue and improved hepatic antioxidant system and hepatocellular architecture. Overall, silymarin and quercetin showed better effects than catechin and genistein in reducing hepatic dysfunctions induced by GEN in rats.
本研究旨在探讨槲皮素、儿茶素、水飞蓟素和染料木素等不同植物化学物质对庆大霉素(GEN)急性中毒大鼠肝脏抗氧化系统和组织学的调节作用。成年Wistar大鼠66只,随机分为11组。给药后血浆AST、ALT、GGT和ALP活性呈剂量依赖性升高(p < 0.05),提示大鼠出现急性肝功能障碍。肝组织中丙二醛(MDA)和高级氧化蛋白产物(AOPP)水平升高(p < 0.05),总抗氧化状态(TAS)、总硫醇(TTH)、还原性谷胱甘肽(GSH)、谷胱甘肽还原酶(GR)、谷胱甘肽过氧化物酶(GPx)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性降低(p < 0.05),表明肝损伤的强度。这些观察结果伴随着各种组织病理学变化,如严重萎缩肝细胞附近的炎症细胞浸润,退行性改变,空泡变性或肝脏实质完全丧失,以剂量依赖的方式。同时暴露于植物化学物质和GEN显著(p < 0.05)减轻肝组织损伤,肝组织中MDA和AOPP水平降低,肝抗氧化系统和肝细胞结构改善。综上所述,水飞蓟素和槲皮素在减轻GEN致大鼠肝功能障碍方面的作用优于儿茶素和染料木素。
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引用次数: 0
Evaluating the antidiabetic properties of microencapsulated phenolic compounds from Peganum harmala in alloxan-induced diabetic Balb/c mice 四氧嘧啶诱导的糖尿病小鼠Balb/c的抗糖尿病作用
Pub Date : 2025-11-18 DOI: 10.1016/j.focha.2025.101166
Zahra Kadkhoda Mezerji , Maryam Nezami , Afagh sadeghizadeh , Seyedeh Negin Sadat Madarshahi , Seyedeh Arghavan Amini , Mahsa Rastegar Moghaddam Poorbagher , Negin Shafaei , Ehsan Karimi , Ehsan Oskoueian
Diabetes mellitus is a complex hormonal disorder marked by high blood sugar, resulting from inadequate insulin secretion, pancreatic β cell damage, or insulin resistance. With over 400 million affected globally, it has reached epidemic proportions. This study explores the microencapsulation of phenolic-rich fractions of Peganum harmala (PRF-PH) and their impact on diabetic mice induced with alloxan, highlighting potential improvement in treatment efficacy. Analysis showed that synthesized microcapsules have a spherical shape with an average diameter of 375 nm and a polydispersity index of 0.37. Administering PRF-PH at 100 mg/kg significantly improved weight gain and feed intake in diabetic mice (p < 0.05). PRF-PH treatment also normalized blood glucose levels, lipid profiles, and liver enzyme activities (p < 0.05). There was an increase in catalase (CAT) expression and a decrease in inducible nitric oxide synthase (iNOS) expression, along with increased glucose transporters GLUT-2 and GLUT-4 (p ≤ 0.05). These findings suggest that PRF-PH may alleviate symptoms of alloxan-induced diabetes and could serve as a potential dietary supplement for diabetes management.
糖尿病是一种以高血糖为特征的复杂激素紊乱,由胰岛素分泌不足、胰腺β细胞损伤或胰岛素抵抗引起。全球有4亿多人受到影响,已达到流行病的程度。本研究探讨了富酚组分槟榔(Peganum harmala, PRF-PH)的微胶囊化及其对四氧嘧啶诱导的糖尿病小鼠的影响,强调了其治疗效果的潜在改善。分析表明,合成的微胶囊呈球形,平均直径为375 nm,多分散性指数为0.37。给药100 mg/kg的PRF-PH显著提高了糖尿病小鼠的增重和采食量(p < 0.05)。PRF-PH治疗也使血糖水平、血脂和肝酶活性正常化(p < 0.05)。过氧化氢酶(CAT)表达升高,诱导型一氧化氮合酶(iNOS)表达降低,葡萄糖转运体GLUT-2和GLUT-4表达升高(p≤0.05)。这些发现表明,PRF-PH可能减轻四氧嘧啶诱导的糖尿病症状,并可能作为糖尿病管理的潜在膳食补充剂。
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引用次数: 0
Integration of ultrasound-assisted extraction and deep eutectic solvent for enhanced recovery of bioactive compounds: Innovative green approach 超声辅助萃取和深度共熔溶剂的结合,提高生物活性化合物的回收率:创新的绿色方法
Pub Date : 2025-11-17 DOI: 10.1016/j.focha.2025.101153
Monika Roy , Piyush Kashyap , Kshirod Kumar Dash , Rafeeya Shams , G.V.S. BhagyaRaj , Shaikh Ayaz Mukarram , Béla Kovács
The use of Deep Eutectic Solvents (DESs) in conjunction with advanced extraction techniques, such as ultrasound-assisted extraction (UAE), can significantly enhance the effectiveness, specificity, and environmental friendliness of extraction procedures. Ultrasound can induce cavitation and microstreaming in the DES, which facilitates the solvent's penetration of the matrix and the liberation of target compound from solid substrates. This augmented mass transfer can result in increased extraction yields and shorter extraction times compared to traditional approaches. DESs are environmentally friendly and sustainable alternatives to traditional solvents due to their ability to degrade naturally, low toxicity levels, and renewable constituents. This review analyzes the process of extracting bioactive compounds from agro-industrial food waste using eco-friendly solvents and the integration of UAE and DESs for their enhanced recovery. Additionally, it encompassed the utilization of UAE and DESs to enhance the beneficial properties of bioactive compounds, such as antioxidants, antimicrobials, and antibacterial agents, as well as the potential viability of using these emerging solvents with UAE for the extraction process. Therefore, by leveraging the distinctive characteristics of DESs and the mechanical impact of ultrasound, this combined method can transform the extraction process, enabling the creation of environmentally friendly and effective extraction methods for food processing.
深层共晶溶剂(DESs)与超声辅助提取(UAE)等先进提取技术相结合,可以显著提高提取过程的有效性、特异性和环境友好性。超声波可以在DES中诱导空化和微流,这有利于溶剂穿透基体,使目标化合物从固体基质中解放出来。与传统方法相比,这种增强的传质可以提高提取收率,缩短提取时间。由于其自然降解能力、低毒性水平和可再生成分,DESs是传统溶剂的环保和可持续替代品。本文综述了利用环保型溶剂从农业工业食品垃圾中提取生物活性化合物的工艺,以及阿联酋和DESs的结合提高其回收率的方法。此外,它还包括利用UAE和DESs来增强生物活性化合物的有益特性,如抗氧化剂、抗菌剂和抗菌剂,以及将这些新兴溶剂与UAE一起用于提取过程的潜在可行性。因此,通过利用DESs的独特特性和超声波的机械影响,这种组合方法可以改变提取过程,从而为食品加工创造环保有效的提取方法。
{"title":"Integration of ultrasound-assisted extraction and deep eutectic solvent for enhanced recovery of bioactive compounds: Innovative green approach","authors":"Monika Roy ,&nbsp;Piyush Kashyap ,&nbsp;Kshirod Kumar Dash ,&nbsp;Rafeeya Shams ,&nbsp;G.V.S. BhagyaRaj ,&nbsp;Shaikh Ayaz Mukarram ,&nbsp;Béla Kovács","doi":"10.1016/j.focha.2025.101153","DOIUrl":"10.1016/j.focha.2025.101153","url":null,"abstract":"<div><div>The use of Deep Eutectic Solvents (DESs) in conjunction with advanced extraction techniques, such as ultrasound-assisted extraction (UAE), can significantly enhance the effectiveness, specificity, and environmental friendliness of extraction procedures. Ultrasound can induce cavitation and microstreaming in the DES, which facilitates the solvent's penetration of the matrix and the liberation of target compound from solid substrates. This augmented mass transfer can result in increased extraction yields and shorter extraction times compared to traditional approaches. DESs are environmentally friendly and sustainable alternatives to traditional solvents due to their ability to degrade naturally, low toxicity levels, and renewable constituents. This review analyzes the process of extracting bioactive compounds from agro-industrial food waste using eco-friendly solvents and the integration of UAE and DESs for their enhanced recovery. Additionally, it encompassed the utilization of UAE and DESs to enhance the beneficial properties of bioactive compounds, such as antioxidants, antimicrobials, and antibacterial agents, as well as the potential viability of using these emerging solvents with UAE for the extraction process. Therefore, by leveraging the distinctive characteristics of DESs and the mechanical impact of ultrasound, this combined method can transform the extraction process, enabling the creation of environmentally friendly and effective extraction methods for food processing.</div></div>","PeriodicalId":73040,"journal":{"name":"Food chemistry advances","volume":"9 ","pages":"Article 101153"},"PeriodicalIF":0.0,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145579292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A review of the most important microRNAs involved in fruit postharvest properties 参与水果采后特性的最重要的microrna综述
Pub Date : 2025-11-15 DOI: 10.1016/j.focha.2025.101168
Seyed Mohammad Samadi , Keyvan Asefpour Vakilian , Seyed Mohamad Javidan
Although extensive studies have been conducted on fruit microRNAs, they have mostly focused on microRNA identification and target gene prediction and validation. This review aims to find connections between microRNAs and postharvest properties of fruits based on publications over the last two decades. The postharvest characteristics investigated in this work include fruit ripening and aging, fruit color and browning, fruit sugars and organic acids, and fruit texture. Although an in-depth analysis of downstream signaling pathways is not provided in this study, it is an effort to identify which fruits and which microRNA families have received the most research. This review reveals that miR156, miR164, miR172, miR319, miR396, miR397, miR399, and miR828, with target genes of SPL, MYB, NAC, BGA, LAC, and ACO, have been investigated the most in previous studies. Moreover, it suggests that although microRNAs have essential effects on fruit quality regulation, current research has focused on a limited number of fruit models. Ongoing research on microRNAs in various fruit types will expand our knowledge of their regulatory functions, providing valuable insights into their role in fruit growth and ripening processes.
虽然对水果microRNA进行了大量的研究,但主要集中在microRNA的鉴定和靶基因的预测和验证。本综述旨在根据近二十年来的出版物,寻找microrna与水果采后特性之间的联系。本研究研究的采后特征包括果实的成熟和老化、果实的颜色和褐变、果实的糖和有机酸以及果实的质地。虽然本研究没有提供下游信号通路的深入分析,但它是一项努力,以确定哪些水果和哪些microRNA家族得到了最多的研究。综述发现,在以往的研究中,以SPL、MYB、NAC、BGA、LAC和ACO为靶基因的miR156、miR164、miR172、miR319、miR396、miR397、miR399和miR828研究最多。此外,这表明尽管microRNAs在水果质量调控中具有重要作用,但目前的研究主要集中在有限的水果模型上。对各种水果中microrna的持续研究将扩大我们对其调控功能的认识,为它们在水果生长和成熟过程中的作用提供有价值的见解。
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引用次数: 0
Untangling the biological efficacy and chemical composition of different solvent extractives from Brownlowia elata Roxb. leaves 褐花不同溶剂提取物的生物药效及化学成分研究。叶子
Pub Date : 2025-11-14 DOI: 10.1016/j.focha.2025.101160
Md. Helal Uddin Chowdhury, Shaikh Bokhtear Uddin, Mohammad Omar Faruque
Traditionally, Brownlowia elata Roxb. is employed to treat syphilis and diarrhea; henceforth bioactive potential of this remains unexplored. Its effectiveness in treating helminthic and bacterial-mediated diarrhea alongside antioxidant and cytotoxic potential unfolds for the first time. Among solvent extractives, BuOH BEL contains a broad spectrum of total bioactive contents. These same extracts had paramount total antioxidant capacity and exhibited an IC50 of 26.3µg/ml in the DPPH assay, as well as a lower cytotoxicity of 38.9µg/ml LC50 value. These bioefficiencies are positively correlated with the total bioactive contents present in this plant, as evidenced by the Pearson correlation and PCA analysis. Similarly, 16.5 and 15 mm furthest inhibitory zones were noted with the same BuOH BEL extract, along with taking 0.823 and 1.64 min lower time for paralysis and death, respectively, comparable to levamisole in an anthelmintic assay. GC-MS analysis of this extract uncovered six fatty acyl, prenol lipids, benzene, and steroid derivative compounds. Among them, dodecyl-phthalate and (25S)-3beta-Hydroxycholest-5-en-26-oic acid methyl ester showed prominent binding efficacy against each of the disease-linked receptors, aiming for potent drug-likeness attributes. To this extent, these findings corroborated the plant’s significant anthelmintic and antibacterial activity, which connects to alleviating diarrhea alongside having antioxidative potential.
传统上,褐花是一种植物。用于治疗梅毒和腹泻;因此,其生物活性潜力仍未开发。它在治疗蠕虫和细菌介导的腹泻以及抗氧化和细胞毒性方面的有效性首次展现。在溶剂萃取物中,BuOH BEL含有广谱的总生物活性成分。这些提取物具有最高的总抗氧化能力,在DPPH实验中显示出26.3µg/ml的IC50,以及38.9µg/ml的低细胞毒性LC50值。这些生物效率与该植物中存在的总生物活性含量呈正相关,Pearson相关和PCA分析证明了这一点。同样,使用相同的BuOH BEL提取物可观察到16.5和15 mm的最远抑制区,同时在驱虫试验中,麻痹和死亡时间分别缩短0.823和1.64分钟,与左旋咪唑相当。该提取物的GC-MS分析揭示了六种脂肪酰基,prenol脂质,苯和类固醇衍生物化合物。其中,邻苯二甲酸十二烷基酯和(25S)-3 β -羟基胆碱-5-烯-26-酸甲酯对每种疾病相关受体的结合效果突出,旨在获得有效的药物相似属性。在这种程度上,这些发现证实了该植物显著的驱虫药和抗菌活性,这与减轻腹泻以及具有抗氧化潜力有关。
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引用次数: 0
Demonstrating the sustainable approach in valorizing the Citrus limetta peel 示范可持续发展的方法,使柠檬皮增值
Pub Date : 2025-11-13 DOI: 10.1016/j.focha.2025.101171
Bawin Ram , Harsh B Jadhav , Sandhya R Shewale , Uday S Annapure
The present study details using sweet lime (Citrus limetta) peel waste in a sequential extraction process to obtain essential oil, hesperidin, and pectin to align with sustainable practices and promote a circular economy. The flavedo portion of the peel was shredded carefully and used for extraction of essential oil using steam and hydro distillation method, while albedo was used for extraction of pectin and hesperidin. The residue obtained after each extraction was used as raw material for the extraction of pectin in a sequential approach. Method of essential oil extraction significantly affected the oil’s composition, d-limonene was the major compound, comprising 67.85% of hydro-distilled oil and 85.46% of steam-distilled oil. Hesperidin extraction involved three different solvents and two distinct extraction temperatures, with the highest yield of ∼0.97% achieved using green solvent ethanol in hot extraction. While high-performance liquid chromatography identified 9.07 µg/mg of hesperidin in extracts obtained through room-temperature ethanol extraction. Pectin extraction compared four acids, with hydrochloric acid yielding the highest 25.58% pectin. Extraction of pectin from different residues resulted in the highest extraction yield of 25.1% from albedo and 21.8% and 8.71% pectin yield from the distillation and hesperidin residues.
本研究详细介绍了在顺序提取过程中使用甜石灰(Citrus limetta)果皮废料来获得精油、橙皮苷和果胶,以符合可持续实践并促进循环经济。将果皮中的黄酮类部分仔细切碎,用蒸汽蒸馏法提取精油,用反照率法提取果胶和橙皮苷。每次提取后得到的残渣依次作为提取果胶的原料。精油提取方法对油的成分有显著影响,d-柠檬烯为主要化合物,在水馏油中占67.85%,在蒸汽蒸馏油中占85.46%。橙皮苷的提取涉及三种不同的溶剂和两种不同的提取温度,采用绿色溶剂乙醇热提取的橙皮苷得率最高,为0.97%。高效液相色谱法鉴定室温乙醇提取液中橙皮苷含量为9.07µg/mg。果胶提取比较了四种酸,盐酸的果胶收率最高,为25.58%。从不同残留物中提取果胶,反照率提取得率最高,为25.1%,蒸馏和橙皮苷提取得率分别为21.8%和8.71%。
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引用次数: 0
Identifying marker compounds for elevated storage temperature in orange juice using untargeted LC-Orbitrap analysis 用非靶向LC-Orbitrap分析鉴定橙汁中贮藏温度升高的标记化合物
Pub Date : 2025-11-09 DOI: 10.1016/j.focha.2025.101163
Patrick Casey, Ciarán Beirne, Damian McNeely, Finbarr Murphy
In this study Untargeted LCOrbitrap analysis is used to identify marker compounds which can be used to identify when orange juice beverages have been stored at elevated temperature. Orange juice (OJ) products, with varying levels of Concentrate % and Pulp %, were held at 40 °C for one week to replicate the effects of improper storage. Using untargeted analysis, it was found that this temperature results in a significant increase of flavonoids tangeretin and nobiletin in all sample types measured. Further analysis shows that the increase in flavonoid concentration is closely correlated to the % of orange juice concentrate in the beverage. Further discussion details how these results support and enhance previous studies which have proposed a mechanism for the release of phenolic compounds during heating. It is also shown that the flavonoid compounds hesperidin and naringin did not exhibit repeatable changes following 40 °C storage, in comparison to control samples stored at 4 °C. The contrasting behaviour of hesperidin and naringin compared to tangeretin and nobiletin is also discussed. Potential benefits for using tangeretin and nobiletin as analytical markers for elevated storage temperature, along with the effectiveness of LCOrbitrap for untargeted analysis are also discussed.
在这项研究中,非靶向LCOrbitrap分析用于识别标记化合物,这些化合物可用于识别橙汁饮料在高温下储存的时间。橙汁(OJ)产品,具有不同水平的浓缩%和果肉%,在40°C下保存一周,以复制不适当储存的效果。使用非靶向分析,发现该温度导致所有样品类型中黄酮类橘子皮素和诺比利素的显著增加。进一步分析表明,黄酮类化合物浓度的增加与饮料中浓缩橙汁的百分比密切相关。进一步的讨论详细说明了这些结果如何支持和加强了先前的研究,这些研究提出了加热过程中酚类化合物释放的机制。研究还表明,与保存在4°C的对照样品相比,在40°C储存后,类黄酮化合物橙皮苷和柚皮苷没有出现可重复的变化。还讨论了橙皮苷和柚皮苷与橘皮素和白皮素的对比行为。本文还讨论了橘皮素和白皮素作为贮藏温度升高的分析标记物的潜在好处,以及LCOrbitrap在非靶向分析中的有效性。
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引用次数: 0
Integrated chemical profiling of Camellia sinensis teas marketed in Brazil: Physicochemical, mineral, and volatile compound characterization 在巴西销售的茶树茶的综合化学分析:物理化学、矿物和挥发性化合物表征
Pub Date : 2025-11-06 DOI: 10.1016/j.focha.2025.101154
Natalie da Silva Aragão , Midori Nakamura Marques , Bruna Ribeiro de Lima , Ariane de Aguiar Queiros , Esther Belo de Souza , Raimundo Carlos Pereira Júnior , Wallice Paxiúba Duncan , Jaime Paiva Lopes Aguiar , Vanessa de Souza Marinho , Francisca das Chagas do Amaral Souza
Tea derived from Camellia sinensis is among the most widely consumed beverages worldwide, valued for its sensory diversity and health-promoting properties. This study aimed to perform a comprehensive chemical characterization of fifteen commercially available teas marketed in Brazil, focusing on their proximate composition, mineral content, and volatile profile. Analytical determinations included physicochemical parameters, macronutrient quantification, and headspace solid-phase microextraction followed by gas chromatography–mass spectrometry (HS-SPME/GC–MS). A total of 298 volatile and semi-volatile compounds were identified, mainly consisting of aldehydes, alcohols, esters, ketones, and terpenes. Principal component analysis (PCA) demonstrated clear clustering of teas according to processing type, particularly differentiating roasted and oxidized samples. The integration of nutritional and volatile data revealed that both processing intensity and geographical origin strongly influence compositional patterns. These findings provide a basis for authenticity assessment, product standardization, and quality control of Brazilian teas, contributing to the development of value-added functional beverages.
从茶树中提取的茶是世界上消费最广泛的饮料之一,因其感官多样性和促进健康的特性而受到重视。本研究旨在对在巴西销售的15种市售茶进行全面的化学表征,重点关注它们的近似成分、矿物质含量和挥发性特征。分析测定包括理化参数、常量营养素定量和顶空固相微萃取-气相色谱-质谱(HS-SPME/ GC-MS)。共鉴定出298种挥发性和半挥发性化合物,主要由醛类、醇类、酯类、酮类和萜类组成。主成分分析(PCA)表明,根据加工类型,茶叶明显聚类,特别是区分烘烤和氧化样品。营养和挥发性数据的综合显示,加工强度和地理来源都强烈影响成分模式。这些研究结果为巴西茶的真实性评估、产品标准化和质量控制提供了依据,有助于开发增值功能饮料。
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Food chemistry advances
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