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Investigation of chemical constituents, antioxidant, anti-inflammatory and nutritional properties of oil of Persea americana (Avocado) seeds 牛油果种子油的化学成分、抗氧化、抗炎和营养特性研究
Pub Date : 2024-07-06 DOI: 10.1016/j.focha.2024.100770
Uchechi Bliss Onyedikachi , Chukwuebuka Callistus Nkwocha , Emmanuel Ejiofor , Chukwu Charles Nnanna

This study investigated the phytochemical composition, in vitro antioxidant, and anti-inflammatory properties of the oil of Persea americana seeds (OPAS). The flavonoid and total phenol compositions were quantified using standard colorimetric methods; the antioxidant potentials of the seed were determined using 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays, while the anti-inflammatory potentials were investigated using membrane stabilization (hypotonicity) and heat-induced hemolysis of human red blood cells (HRBC) assays. In addition, gas chromatography-mass spectrometry (GC–MS) analysis was employed to characterize the phytochemicals in the oil. The nutritional indices (atherogenic index, thrombogenic index, and polyene index) were also calculated. Results indicated high total phenolic and flavonoid contents (25.78 ± 0.68 mg gallic acid equivalent per gram (GAE/g) and 72.69 ± 1.11 mg quercetin equivalent per 100 mg (QE/100 mg), respectively). The oil of P. americana seeds showed dose-dependent (25–400 μg/mL) antioxidant and anti-inflammatory activities compared to the standard agents used (olive oil and ascorbic acid). The GC–MS analysis showed the presence of fatty acids including tetradecanoic acid (33.33 %), 9, 12-octadecadienoic acid, methyl ester (20.12 %), n-hexadecenoic acid (7.07 %), and oleic acid (11.60 %). Nutritional indices showed that OPAS had a very low polyunsaturated/saturated (PUFA/SFA) ratio (0.45), hypocholesterolemic/hypercholesterolemic (0.26), but high atherogenicity index (IA) (4.13) and thrombogenicity index IT (9.47) values. The beneficial potentials of OPAS revealed in this study suggest that it can be harnessed as a potent antioxidant and anti-inflammatory agent in phytomedicine. However, the nutritional quality and culinary suitability of OPAS with different oils require further study.

本研究调查了美洲鲈鱼籽油(OPAS)的植物化学成分、体外抗氧化和抗炎特性。采用标准比色法对黄酮类化合物和总酚成分进行了定量;采用 2,2-二苯基-1-苦基肼(DPPH)和铁还原抗氧化力(FRAP)测定法确定了种子的抗氧化潜力;采用膜稳定(低渗)和热诱导人红细胞溶血(HRBC)测定法研究了抗炎潜力。此外,还采用气相色谱-质谱(GC-MS)分析法对油中的植物化学物质进行了表征。还计算了营养指数(致动脉粥样硬化指数、致血栓指数和多烯指数)。结果表明,总酚和类黄酮含量较高(分别为每克 25.78 ± 0.68 毫克没食子酸当量(GAE/g)和每 100 毫克 72.69 ± 1.11 毫克槲皮素当量(QE/100 毫克))。与所使用的标准制剂(橄榄油和抗坏血酸)相比,美洲金雀花种子油显示出剂量依赖性(25-400 μg/mL)抗氧化和抗炎活性。气相色谱-质谱(GC-MS)分析表明,橄榄油中含有脂肪酸,包括十四烷酸(33.33%)、9, 12-十八碳二烯酸甲酯(20.12%)、正十六烯酸(7.07%)和油酸(11.60%)。营养指数显示,OPAS 的多不饱和/饱和(PUFA/SFA)比值(0.45)、低胆固醇/高胆固醇比值(0.26)很低,但动脉粥样硬化指数(IA)(4.13)和血栓形成指数 IT(9.47)很高。本研究揭示的 OPAS 的有益潜力表明,它可以作为一种有效的抗氧化剂和抗炎剂用于植物药。不过,还需要进一步研究 OPAS 与不同油类的营养质量和烹饪适宜性。
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引用次数: 0
Talinum triangulare (Jacq.) Willd: A review of its traditional uses, phytochemistry, and pharmacology along with network pharmacology analysis of its components and targets Talinum triangulare (Jacq.) Willd:对其传统用途、植物化学和药理学的综述,以及对其成分和靶点的网络药理学分析
Pub Date : 2024-07-05 DOI: 10.1016/j.focha.2024.100768
Dipankar Barman , K Nusalu Puro , Jyoti Lakshmi Hati Boruah , Deepak Kumar , Kalyani Medhi , Bhaskar Mazumder , Rinku Baishya

Talinum triangulare (water leaf) is an important herbaceous vegetable with medicinal importance. It has been used in traditional medicine for the management of diabetes, cancer, stroke, obesity, and measles. Popularly considered a weed, it is an unconventional food plant (UFP) with many nutritional and socio-economic potentials that needs to be rescued for invasive farming. The plant is a reservoir of various phytoconstituents belonging to flavonoids and lignans, alkaloids, and benzoic acid derivatives, which are known for their wide range of pharmacological effects. There is tremendous therapeutic potential in this cosmopolitan leafy vegetable plant, which has antidiabetic, antioxidative, hepatoprotective, and anticancer activity, along with a plethora of other pharmacological activities. This article reviews the traditional uses, phytochemistry, and pharmacology aspects of this underutilized food plant based on an extensive literature search which was available from 1976 to 2024 using a variety of electronic search engines. A network pharmacology-based approach was used for validation of the traditional claims as well as for exploring its therapeutic potential against diabetes for future research directions. This review intends to sensitize the scientific community to further research on this promising plant to bring it to commercial utilization as well as broaden the scope of this underutilized food plant.

Talinum triangulare(水叶)是一种重要的药用草本植物。在传统医学中,它被用于治疗糖尿病、癌症、中风、肥胖症和麻疹。人们普遍认为它是一种杂草,但它是一种非常规食用植物(UFP),具有许多营养和社会经济潜力,需要抢救以用于入侵性耕作。该植物蕴藏着各种植物成分,包括黄酮类、木脂素、生物碱和苯甲酸衍生物,具有广泛的药理作用。这种世界性的叶菜植物具有巨大的治疗潜力,具有抗糖尿病、抗氧化、保肝和抗癌活性,以及大量其他药理活性。本文通过使用各种电子搜索引擎对 1976 年至 2024 年期间的大量文献进行了检索,在此基础上对这种未被充分利用的食用植物的传统用途、植物化学和药理学方面进行了综述。本综述采用了基于网络药理学的方法来验证传统说法,并探索其对糖尿病的治疗潜力,以确定未来的研究方向。本综述旨在促使科学界进一步研究这种前景广阔的植物,使其得到商业利用,并扩大这种未得到充分利用的食用植物的范围。
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引用次数: 0
Biosynthesis, antimicrobial and in vitro antiproliferative activities of silver/silver chloride nanoparticles from mixed fruit extracts of Capsicum frutescens and Tamarindus indica 辣椒和罗望子混合果实提取物中银/氯化银纳米粒子的生物合成、抗菌和体外抗增殖活性
Pub Date : 2024-07-05 DOI: 10.1016/j.focha.2024.100773
Ramim Hasan Naim , Md. Belal Uddin , Syed Rashel Kabir , A. K. M. Asaduzzaman , Ranajit Kumar Shaha , Imtiaj Hasan

Silver/silver chloride nanoparticles were formed by treating mixed extracts of Capsicum frutescens and Tamarindus indica (CT) with 5 mM AgNO3 in sunlight for 120 min, which was confirmed by a UV–visible spectrophotometer as a comparatively sharp peak was observed at 465 nm. Crystalline properties of Ag/AgCl-NPs were examined through X-ray diffraction method. The nearly spherical shape of Ag/AgCl-NPs was confirmed by scanning electron microscopy and the average size of particle was approximately 72.2 nm. The existence of elemental Ag was assessed with an energy-dispersive spectrometer whereas two major functional groups as capping agents were obtained using Fourier transform infrared spectroscopy. The CT extract-mediated Ag/AgCl-NPs showed moderate toxicity determined against brine shrimp nauplii with an LD50 value of 177.9 μg/mL. The nanoparticles showed 92.6 % growth-suppressive activity against S. aureus, 81.1 % against S. boydii, 60.3 % against S. dysenteriae, and 50 % against E. coli at the concentration of 50 µg/mL in disc diffusion assay. The recorded free radical scavenging activities of Ag/AgCl-NPs were 55.79 % at 60 µg/mL for DPPH and 64.93 % at 100 µg/mL for ABTS. MTT colorimetric assay confirmed the antiproliferative activity of Ag/AgCl nanoparticles against EAC and MCF-7 cells (42.7 % and 47.4 %) at concentrations of 50 µg/mL and 128 µg/mL, respectively.

将辣椒和罗望子(CT)的混合提取物用 5 mM AgNO3 在阳光下处理 120 分钟后,形成了银/氯化银纳米粒子,经紫外可见分光光度计确认,在 465 纳米处观察到一个比较尖锐的峰。通过 X 射线衍射法检测了 Ag/AgCl-NPs 的晶体性质。扫描电子显微镜证实,Ag/AgCl-NPs 几乎呈球形,颗粒的平均尺寸约为 72.2 nm。利用能量色散光谱仪评估了银元素的存在,而利用傅里叶变换红外光谱则获得了作为封端剂的两个主要官能团。CT 提取物介导的 Ag/AgCl-NPs 对盐水虾稚虫具有中等毒性,半数致死剂量为 177.9 μg/mL。在盘扩散试验中,当浓度为 50 微克/毫升时,纳米粒子对金黄色葡萄球菌的生长抑制活性为 92.6%,对痢疾杆菌的生长抑制活性为 81.1%,对大肠杆菌的生长抑制活性为 60.3%,对大肠杆菌的生长抑制活性为 50%。在 60 微克/毫升的 DPPH 浓度下,Ag/AgCl-NPs 的自由基清除活性为 55.79%;在 100 微克/毫升的 ABTS 浓度下,Ag/AgCl-NPs 的自由基清除活性为 64.93%。MTT 比色法证实了 Ag/AgCl 纳米粒子在浓度为 50 µg/mL 和 128 µg/mL 时对 EAC 和 MCF-7 细胞的抗增殖活性(分别为 42.7% 和 47.4%)。
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引用次数: 0
Characterization of collagen and its hydrolysate from southern bluefin tuna skin and their potencies as DPP-IV inhibitors 南方蓝鳍金枪鱼表皮胶原蛋白及其水解物的特征及其作为 DPP-IV 抑制剂的功效
Pub Date : 2024-07-04 DOI: 10.1016/j.focha.2024.100774
Erwina Safitri , Olivia H. Kuziel , Takeshi Nagai , Masataka Saito

Collagen-derived peptides have been gaining interest due to their potential to act as DPP-IV inhibitors, which are considered to have antidiabetic effects. This study focused on extracting type I collagen from discarded southern bluefin tuna skin, confirming its structure and properties, and then measuring the ability of its hydrolysates to act as DPP-IV inhibitors. First, type I collagen was purified from southern bluefin tuna skin, and the constituent subunit was found to contain the α3(I) chain. Next, enzymatic hydrolysates were prepared, and their DPP-IV inhibitory activity measurements were examined and compared. Collagenase hydrolysate showed the highest DPP-IV inhibitory activity (71.8 ± 0.3 %), and considering digestive degradation, collagenase-pepsin-trypsin hydrolysate was prepared and separated into fractions via anion exchange chromatography, cartridge column, and HPLC. Notably, the 70 % methanol eluted fraction separated via cartridge column was found to have an IC50 of 0.26 ± 0.01 mg/ml. Subsequently, a novel peptide GPSGGGYDV with the highest DPP-IV inhibitory activity (IC50 value 82±0.0 µM) was purified from this fraction and found to be a competitive inhibitor of DPP-IV. This is the first study to investigate the DPP-IV inhibitory potential of type I collagen hydrolysate from southern bluefin tuna.

胶原蛋白肽具有作为 DPP-IV 抑制剂的潜力,被认为具有抗糖尿病作用,因此越来越受到人们的关注。本研究的重点是从废弃的南方蓝鳍金枪鱼皮中提取 I 型胶原蛋白,确认其结构和特性,然后测定其水解物作为 DPP-IV 抑制剂的能力。首先,从南方蓝鳍金枪鱼皮中纯化出 I 型胶原蛋白,发现其组成亚基包含 α3(I)链。接着,制备了酶水解物,并对其 DPP-IV 抑制活性进行了检测和比较。胶原酶水解物显示出最高的 DPP-IV 抑制活性(71.8 ± 0.3 %),考虑到消化降解,制备了胶原酶-胃蛋白酶-胰蛋白酶水解物,并通过阴离子交换色谱、滤芯柱和高效液相色谱进行了馏分分离。值得注意的是,通过滤芯柱分离的 70% 甲醇洗脱馏分的 IC50 值为 0.26 ± 0.01 mg/ml。随后,从该馏分中纯化出一种具有最高 DPP-IV 抑制活性(IC50 值为 82±0.0 µM)的新型多肽 GPSGGGYDV,并发现它是一种 DPP-IV 竞争性抑制剂。这是首次研究南部蓝鳍金枪鱼 I 型胶原蛋白水解物的 DPP-IV 抑制潜力。
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引用次数: 0
The effect of microencapsulation of microbial oil containing CLA by the complex coacervation on the physicochemical and sensory characteristics of buttermilk 复合共凝胶法微胶囊化含CLA的微生物油对酪乳理化和感官特性的影响
Pub Date : 2024-07-04 DOI: 10.1016/j.focha.2024.100757
Samin Rafi Azari , Mohammad Hojjatoleslamy , Zeinab E. Mousavi , Hossein Kiani , Sayed Mohammad Ali Jalali

Conjugated Linoleic Acid (CLA) is known for their positive impact on reducing various diseases, including cardiovascular diseases and blood lipid levels. This study aims to produce enriched buttermilk with stable physicochemical properties via complex coacervation. To address the inherent instability of oils in liquid systems, microcapsules were prepared using sodium alginate and xanthan gum, with optimization achieved through the response surface method (RSM). The buttermilk, enriched at optimal levels of 0 %, 0.04 %, 0.07 %, and 0.1 %, was then incubated at 48 °C for 42 days. Under the optimized conditions, the choice and quantity of gums used for enrichment significantly influenced the properties of the enriched buttermilk. A decrease in pH, along with an increase in acidity, led to enhanced viscosity in the buttermilk. The flow behavior of the buttermilk exhibited pseudoplastic characteristics. Enrichment caused an increase in particle size, with only minimal changes in odor, taste, and color parameters, while the buttermilk remained free from protein agglutinates. Chromatography results showed an increase in unsaturated fatty acids after enrichment. In summary, this study demonstrates the feasibility of producing nutritionally enriched buttermilk with high stability and desirable sensory characteristics. The recommended concentrations for enrichment are 0.04 % and 0.07 %.

众所周知,共轭亚油酸(CLA)对减少各种疾病(包括心血管疾病和血脂水平)有积极影响。本研究旨在通过复合共凝固法生产理化性质稳定的浓缩酪乳。为了解决液体体系中油固有的不稳定性,研究人员使用海藻酸钠和黄原胶制备了微胶囊,并通过响应面法(RSM)进行了优化。酪乳的最佳富集水平分别为 0%、0.04%、0.07% 和 0.1%,然后在 48 °C 下培养 42 天。在优化条件下,用于富集的胶质的选择和数量对富集酪乳的特性有显著影响。pH 值的降低和酸度的增加会导致酪乳粘度的增加。酪乳的流动性表现出假塑性特征。富集会导致粒度增大,但气味、味道和颜色参数的变化很小,同时酪乳中仍然没有蛋白质凝集物。色谱结果显示,不饱和脂肪酸在富集后有所增加。总之,这项研究证明了生产具有高稳定性和理想感官特性的营养强化酪乳的可行性。建议的富集浓度为 0.04 % 和 0.07 %。
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引用次数: 0
Exploring the significance of protein concentrate: A review on sources, extraction methods, and applications 探索浓缩蛋白质的意义:来源、提取方法和应用综述
Pub Date : 2024-06-28 DOI: 10.1016/j.focha.2024.100771
Adiba Fatima , Poornima Singh , Vinay Kumar Pandey , Rahul Singh , Sarvesh Rustagi

Proteins are usually composed of carbon, nitrogen, hydrogen, oxygen, and sulfur, resulting in too many amino acid molecules with interlinked peptide bonds. A careful analysis of other protein-rich choices is required because of the depletion of conventional protein resources and the need to meet the dietary demands of the increasing global population. The prime motive for creating concentrates and isolates from a protein source is to increase the concentration of proteins by removing non-protein constituents, allowing the use of a lower amount of protein for food formulation to provide specific nutritional and functional properties. Protein concentrates are a possible alternative that come from a range of sources, including dairy products, plants, and insects. Their extraction strategies, which range from conventional solvent extraction to cutting-edge approaches, such as membrane separation and enzyme-assisted procedures, provide opportunities to maximize yield, maintain nutritional value, and reduce environmental impact. These concentrates are used in the food sector to improve dietary profiles, formulate functional foods, and meet growing demand for plant-based and alternative protein sources. This review advances our understanding of this crucial element in the search for nutrient-dense and sustainable protein sources by clarifying their origins, extraction techniques, and uses.

蛋白质通常由碳、氮、氢、氧和硫组成,因此氨基酸分子过多,肽键相互连接。由于传统蛋白质资源的枯竭,以及需要满足日益增长的全球人口的饮食需求,因此需要对其他富含蛋白质的选择进行仔细分析。从蛋白质来源中提取浓缩物和分离物的主要动机是通过去除非蛋白质成分来提高蛋白质的浓度,从而在食品配方中使用较少的蛋白质来提供特定的营养和功能特性。蛋白质浓缩物是一种可能的替代品,其来源多种多样,包括乳制品、植物和昆虫。它们的提取策略从传统的溶剂萃取到膜分离和酶辅助程序等尖端方法,为最大限度地提高产量、保持营养价值和减少对环境的影响提供了机会。这些浓缩物被用于食品行业,以改善膳食结构、配制功能性食品,并满足人们对植物蛋白和替代蛋白来源日益增长的需求。本综述通过阐明浓缩物的来源、提取技术和用途,加深了我们对这一寻找营养丰富和可持续蛋白质来源的关键要素的了解。
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引用次数: 0
Sustainable gelatin-based packaging with nanoemulsified chilli seed oil for enhancing poultry meat preservation: An eco-friendly approach 使用纳米乳化辣椒籽油的可持续明胶基包装可提高禽肉保鲜效果:生态友好型方法
Pub Date : 2024-06-27 DOI: 10.1016/j.focha.2024.100761
Jahangir A Rather , Gurkirat Kaur , Irshad A Shah , Darakshan Majid , Hilal A Makroo , B.N. Dar

Utilizing poultry feet, a typically underutilized by-product, we extracted gelatin to fabricate environmentally friendly packaging pouches for the shelf life enhancement of fresh chicken breast cubes. The incorporation of chilli seed oil nanoemulsions, characterized by their particle sizes (61-127 nm) and zeta potential values (-18 to -28 mV), not only contributed to the pouches' structural integrity but also imparted antimicrobial properties. These pouches demonstrated reduced UV light transmittance and controlled color changes in packaged chicken breast cubes, compared to the control samples. Significantly, the pouch-packaged samples exhibited slower pH increase, reduced weight loss (16.2-20.4%), and lower TBARS values, indicative of delayed lipid oxidation and spoilage. Microbiological assessments revealed that the packaged samples effectively restrained microbial growth, staying within safety limits for E. coli and total plate counts, unlike the control samples which exceeded these thresholds. Textural analysis further showed better preservation of the meat's consistency within the pouches. This investigation not only highlights the valorization of food industry by-products in developing sustainable packaging solutions but also emphasizes the efficiency of developed packaging pouches in enhancing shelf-life of fresh poultry meat cubes, thus resonating with the contemporary needs of food processing technologies and consumer acceptance.

家禽脚是一种通常未得到充分利用的副产品,我们利用这种副产品提取明胶来制作环保型包装袋,以延长新鲜鸡胸肉块的保质期。辣椒籽油纳米乳液的粒径(61-127 nm)和 ZETA 电位(-18 至 -28 mV)不仅增强了包装袋的结构完整性,还赋予了其抗菌特性。与对照样品相比,这些小袋降低了紫外线透射率,并控制了包装鸡胸肉块的颜色变化。值得注意的是,小袋包装样品的 pH 值升高较慢,重量损失减少(16.2-20.4%),TBARS 值降低,表明脂质氧化和变质的时间推迟。微生物学评估显示,包装样品有效地抑制了微生物的生长,大肠杆菌和菌落总数均未超过安全限值,而对照样品则超过了这些限值。纹理分析进一步表明,小袋包装能更好地保持肉的稠度。这项调查不仅突出了食品工业副产品在开发可持续包装解决方案中的价值,还强调了所开发的包装袋在提高新鲜禽肉块保质期方面的效率,从而与当代食品加工技术的需求和消费者的接受程度产生了共鸣。
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引用次数: 0
Designing Plant-Based Smart Food Packaging Solutions for Prolonging Consumable Life of Perishable Foods 设计基于植物的智能食品包装解决方案,延长易腐食品的食用期限
Pub Date : 2024-06-27 DOI: 10.1016/j.focha.2024.100769
Marzieh Baneshi , Alberta N.A. Aryee , Marcia English , Martin Mkandawire

Concerns about the environmental and health impacts of plastics have driven the search for alternatives to petroleum-based food packaging materials. Simultaneously, the food packaging industry is making rapid progress in developing smart packaging technologies that extend the shelf life and freshness of perishable foods. Among the alternatives, plant-based packaging materials are gaining prominence because of their high environmental, health, and economical properties. However, the advancement in smart packing technologies has mainly involved petroleum-based plastics. Consequently, it is imminent that these smart technologies should be geared toward emerging plant-based packing materials. Hence, this perspective article explores the potential, opportunities, and challenges in designing smart packing materials using plant-based materials like protein, cellophane, and other bio-polymeric plastics. Specifically, we explore how the plant-based platform for smart food packing for prolonging the consumable life of perishable food products can be achieved without losing the desirable properties exhibited by petroleum-based plastic platforms.

对塑料对环境和健康影响的担忧促使人们寻找石油食品包装材料的替代品。与此同时,食品包装行业在开发延长易腐食品保质期和新鲜度的智能包装技术方面也取得了快速进展。在这些替代品中,植物基包装材料因其环保、健康和经济的特性而日益受到重视。然而,智能包装技术的发展主要涉及石油基塑料。因此,将这些智能技术应用于新兴的植物基包装材料迫在眉睫。因此,本视角文章探讨了使用蛋白质、玻璃纸和其他生物聚合塑料等植物基材料设计智能包装材料的潜力、机遇和挑战。具体而言,我们将探讨如何在不失去石油基塑料平台所表现出的理想特性的情况下,利用植物基平台设计智能食品包装,以延长易腐食品的食用寿命。
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引用次数: 0
Effect of heating and pH on hydroxymethylfurfural content, diastase and invertase activity of Dalbergia honey using response surface methodology 利用响应面法研究加热和 pH 值对达尔贝格蜂蜜羟甲基糠醛含量、双酶和转化酶活性的影响
Pub Date : 2024-06-25 DOI: 10.1016/j.focha.2024.100766
Rajni Kamboj , Rubrinder Singh Sandhu , Vikas Nanda

The current study explored the botanical origin of Dalbergia (Dalbergia sissoo) honey source using melissopalynology. The 20 runs in triplicate were evaluated using the composite central rotatable design (CCRD) for the analysis of effect of heating (45–55 ºC), pH (4.8–5.8) and time (5–15 min) on attributes features like hydroxymethylfurfural (HMF) content, diastase & invertase activity of Dalbergia honey with Response Surface Method (RSM). A significant effect of process variables on three responses has btabeen observed from the statistical data. The amount of HMF content increased at higher temperature and pH. However, the diastase activity can be well monitored in the increased temperature. The invertase activity of honey was significantly (P < 0.0001) reduced for every pH value from 4.8 to 5.8. The results attained when heating beyond 49 ºC as well as a higher pH with prolonged time could alter HMF content, diastase and invertase activity, which may deteriorate the grade of honey.

本研究采用蜜源学方法探讨了达尔贝格(Dalbergia sissoo)蜜源的植物学来源。采用复合中央可旋转设计(CCRD)对一式三份的 20 次试验进行了评估,以分析加热(45-55 ºC)、pH 值(4.8-5.8)和时间(5-15 分钟)对 Dalbergia 蜂蜜的羟甲基糠醛(HMF)含量、双酶& 和转化酶活性等属性特征的影响。从统计数据中可以看出,工艺变量对三种反应有明显的影响。温度和 pH 值越高,HMF 含量越高。然而,在温度升高的情况下,双酶活性可以得到很好的监测。从 4.8 到 5.8,pH 值越高,蜂蜜的转化酶活性越低(P< 0.0001)。结果表明,加热温度超过 49 ºC 和 pH 值升高并持续较长时间会改变 HMF 含量、双酶和转化酶活性,从而可能降低蜂蜜的等级。
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引用次数: 0
Protective effect and antioxidative efficacy of Moringa stenopetala leaf extract in BV-2 microglial cell 辣木叶提取物对 BV-2 小胶质细胞的保护作用和抗氧化功效
Pub Date : 2024-06-24 DOI: 10.1016/j.focha.2024.100767
Stephen Adeniyi Adefegha , Vitor Mostardeiro , Vera Maria Morsch , Ademir F. Morel , Ivana Beatrice Manica Da Cruz , Sabrina Somacal , Ganiyu Oboh , Maria Rosa Chitolina Schetinger

Moringa stenopetala is often consumed as food and used in folkloric medicine for the management of several diseases including neurodegenerative diseases. This study investigated the protective effect and antioxidant efficacy of aqueous extract of Moringa stenopetala in BV-2 microglial cells. Aqueous extracts of M. stenopetala were prepared, lyophilized and reconstituted in 0.5% dimethylsulphoxide (DMSO). Cells were treated with M. stenopetala extracts (0.1–100 µg/ml) for cell viability assay and nitric oxide (NO) production tests. However, M. stenopetala extract (50 µg/ml) was used in the treatment of cells for the determination of protein carbonyl content and reactive oxygen species (ROS) level. Incubation of BV-2 microglia cell with M. stenopetala extract maintained cell viability, diminished NO and ROS levels, and reduced protein carbonyl contents. Chlorogenic acid, rutin, kaempferol and quercetin derivatives were the main phenolic compounds identified in M. stenopetala leaf extract. In conclusion, this study suggests that M. stenopetala extract is rich in antioxidant and contains bioactive neuroprotective agents.

Moringa stenopetala 常被作为食物食用,并在民间医药中用于治疗包括神经退行性疾病在内的多种疾病。本研究调查了辣木水提取物对 BV-2 小神经胶质细胞的保护作用和抗氧化功效。研究人员制备了M. stenopetala的水提取物,将其冻干并在0.5%的二甲基亚砜(DMSO)中重构。用石蒜提取物(0.1-100 µg/ml)处理细胞,进行细胞活力测定和一氧化氮(NO)生成测试。然而,在测定蛋白质羰基含量和活性氧(ROS)水平时,则使用石蒜提取物(50 µg/ml)处理细胞。用石杉碱甲提取物培养 BV-2 小胶质细胞可维持细胞活力,降低 NO 和 ROS 水平,并减少蛋白质羰基含量。绿原酸、芦丁、山柰酚和槲皮素衍生物是石蒜叶提取物中的主要酚类化合物。总之,本研究表明石蒜叶提取物富含抗氧化剂和生物活性神经保护剂。
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Food chemistry advances
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