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Omics Profiles of Non-transgenic Scion Grafted on Transgenic RdDM Rootstock 转基因RdDM砧木嫁接非转基因接穗组学分析
Pub Date : 2022-03-01 DOI: 10.14252/foodsafetyfscj.D-21-00012
H. Kodama, Yukiko Umeyama, Taira Miyahara, Taichi Oguchi, Takashi Tsujimoto, Y. Ozeki, Takumi Ogawa, Y. Yamaguchi, D. Ohta
Abstract Grafting of commercial varieties onto transgenic stress-tolerant rootstocks is attractive approach, because fruit from the non-transgenic plant body does not contain foreign genes. RNA silencing can modulate gene expression and protect host plants from viruses and insects, and small RNAs (sRNAs), key molecules of RNA silencing, can move systemically. Here, to evaluate the safety of foods obtained from sRNA-recipient plant bodies, we investigated the effects of rootstock-derived sRNAs involved in mediating RNA-directed DNA methylation (RdDM) on non-transgenic scions. We used tobacco rootstocks showing RdDM against the cauliflower mosaic virus (CaMV) 35S promoter. When scions harboring CaMV 35S promoter sequence were grafted onto RdDM-inducing rootstocks, we found that RdDM-inducing sRNAs were only weakly transported from the rootstocks to the scion, and we observed a low level of DNA methylation of the CaMV 35S promoter in the scion. Next, wild-type (WT) tobacco scions were grafted onto RdDM-inducing rootstocks (designated NT) or WT rootstocks (designated NN), and scion leaves were subjected to multi-omics analyses. Our transcriptomic analysis detected 55 differentially expressed genes between the NT and NN samples. A principal component analysis of proteome profiles showed no significant differences. In the positive and negative modes of LC-ESI-MS and GC-EI-MS analyses, we found a large overlap between the metabolomic clusters of the NT and NN samples. In contrast, the negative mode of a LC-ESI-MS analysis showed separation of clusters of NT and NN metabolites, and we detected 6 peak groups that significantly differed. In conclusion, we found that grafting onto RdDM-inducing rootstocks caused a low-level transmission of sRNAs, resulting in limited DNA methylation in the scion. However, the causal relationships between sRNA transmission and the very slight changes in the transcriptomic and metabolomic profiles of the scions remains unclear. The safety assessment points for grafting with RdDM rootstocks are discussed.
摘要将商业品种嫁接到转基因耐胁迫砧木上是一种有吸引力的方法,因为来自非转基因植物体的果实不含外源基因。RNA沉默可以调节基因表达,保护宿主植物免受病毒和昆虫的侵害,而RNA沉默的关键分子小RNA(sRNA)可以系统移动。在这里,为了评估从sRNA受体植物体获得的食物的安全性,我们研究了参与介导RNA定向DNA甲基化(RdDM)的砧木衍生的sRNA对非转基因接穗的影响。我们使用表现出针对花椰菜花叶病毒(CaMV)35S启动子的RdDM的烟草砧木。当携带CaMV 35S启动子序列的接穗嫁接到RdDM诱导的砧木上时,我们发现RdDM诱发的sRNA仅从砧木弱地转运到接穗,并且我们观察到接穗中CaMV 35S启动子的DNA甲基化水平较低。接下来,将野生型(WT)烟草接穗嫁接到RdDM诱导的砧木(指定为NT)或WT砧木(指定的NN)上,并对接穗叶片进行多组学分析。我们的转录组分析在NT和NN样本之间检测到55个差异表达的基因。蛋白质组图谱的主成分分析显示没有显著差异。在LC-ESI-MS和GC-EI-MS分析的阳性和阴性模式中,我们发现NT和NN样品的代谢组簇之间有很大的重叠。相反,LC-ESI-MS分析的阴性模式显示NT和NN代谢物簇的分离,我们检测到6个显著不同的峰组。总之,我们发现嫁接到RdDM诱导的砧木上会导致sRNA的低水平传递,导致接穗中的DNA甲基化有限。然而,sRNA传播与接穗转录组和代谢组谱的微小变化之间的因果关系尚不清楚。讨论了RdDM砧木嫁接的安全性评价要点。
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引用次数: 1
Radiocesium Concentration in Commercially-Available Foods Produced in Japan: 2017-2019 日本市售食品中的放射性铯浓度:2017-2019
Pub Date : 2022-03-01 DOI: 10.14252/foodsafetyfscj.D-21-00011
H. Nabeshi, Masataka Imamura, T. Tsutsumi, Tomomi Maeda, A. Hachisuka, H. Akiyama
Abstract We investigated the concentration of radioactive cesium (r-Cs: 134Cs and 137Cs) in commercially-available foods to confirm the effectiveness of pre-shipment radioactive material inspections mainly conducted by local governments. We focused on selected production areas and foods with high probability of r-Cs detection. To this end, we evaluated 715, 685, and 683 samples using scintillation spectrometer and high-purity germanium γ-spectrometer in fiscal years 2017, 2018, and 2019, respectively. The results accounted for 9 samples (1.3%), 10 samples (1.5%), and 5 samples (0.7%) for each fiscal year exceeded the standard limit of radioactive material (100 Bq/kg as r-Cs concentration for general foods). Although we selected and evaluated foods with high probability of r-Cs detection, percentage of samples exceeding the standard limit in each fiscal year was very low, less than 2% to be exact. This suggests that food management system, including pre-shipment inspections, were effectively functioning. In addition, samples exceeding the standard limit were bound to edible wild plants and wild mushrooms, and log-cultivated mushrooms. The former is consider to be difficult for cultivation/feeding control, and the latter was know to be parts of foods greatly affected by radioactive materials. This suggests that the concentration of r-Cs in these items remains at relatively high levels. In contrast, r-Cs was not detected in items with controalble cultivation/feeding. Based on these observations, it is better to be inspected on more difficult-to-cotrol cultivation/feeding items, in order to achieve further streamlining and improving of inspection efficiency. Our results indicate that r-Cs concentration in commercially-available foods of easy-to cultivation/feeding control, such as general vegetables, fruits, and meat, have been well-controlled in Japan, however, difficult-to-cultivation/feeding control items need to be more paid attention to r-Cs concentrations.
摘要:本研究对市售食品中放射性铯(r-Cs: 134Cs和137Cs)的浓度进行了调查,以证实主要由地方政府进行的装运前放射性物质检查的有效性。我们重点选择了r-Cs检测概率高的生产区域和食品。为此,我们分别在2017、2018和2019财政年度使用闪烁光谱仪和高纯锗γ光谱仪对715、685和683个样品进行了评估。各会计年度有9个样品(1.3%)、10个样品(1.5%)和5个样品(0.7%)的放射性物质超标(一般食品的r-Cs浓度为100 Bq/kg)。虽然我们选择和评估的食品r-Cs检测概率很高,但每个财政年度样品超过标准限值的百分比非常低,准确地说不到2%。这表明食品管理系统,包括装运前检查,有效运作。此外,超标的样品限定在食用野生植物和野生蘑菇以及原木栽培蘑菇中。前者被认为难以种植/饲养控制,而后者则是受放射性物质影响较大的食品的一部分。这表明,这些物品中的r-Cs浓度仍然处于相对较高的水平。相反,在控制栽培/饲养的项目中未检测到r-Cs。基于这些观察结果,最好对更难控制的栽培/饲养项目进行检查,以进一步简化和提高检查效率。研究结果表明,日本普通蔬菜、水果和肉类等易种植/饲养控制的商品食品中r-Cs浓度已得到较好的控制,但难以种植/饲养控制的商品中r-Cs浓度还需进一步关注。
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引用次数: 0
Allergen Labeling for Eggs as Ingredients of Pre-packaged Foods (Food-allergy). 鸡蛋作为预包装食品成分的过敏原标签(食物过敏)。
Pub Date : 2021-12-24 eCollection Date: 2021-12-01 DOI: 10.14252/foodsafetyfscj.D-21-00028

Basic Act on allergic diseases measures was enforced in 2015, in order to improve the living environment of Japanese population via enhancing food labeling of concerned allergic ingredients. Food Safety Commission of Japan (FSCJ), then, deemed it necessary to examine this Japanese allergen labeling system. Labeling is currently mandatory for 7 ingredients, and in addition, recommended for 21 distinct ingredients. FSCJ chose to conduct a self-tasking risk assessment on hen-eggs labeling, as hen-eggs show high prevalence of allergy cases out of those presented ingredients. Hen-eggs were specifically focused on this assessment due to the available amount of data. There have been no incidents of hen-egg protein-derived allergic reactions at levels below the "threshold concentration", which was set at 10 μg of allergenic protein per 1 g of food for the labeling purpose of Japan's system. On the practical aspect, allergen contamination in pre-packaged foods is continuously prevented through good hygiene practices. Introduction of mandatory HACCP-based approach in the food industry of Japan contributed to appropriate controls, including prevention of labeling errors among others. In conclusion, FSCJ judged the current allergen labeling system on hen-eggs in Japan to be generally appropriate based on the currently available evidences.

《变态反应性疾病对策基本法》于2015年实施,旨在通过加强相关变态反应性成分的食品标识,改善日本民众的生活环境。因此,日本食品安全委员会(FSCJ)认为有必要对日本的过敏原标签制度进行审查。目前,7种成分的标签是强制性的,另外,推荐21种不同的成分。FSCJ选择对母鸡鸡蛋标签进行自我任务风险评估,因为母鸡鸡蛋显示出对这些成分过敏的高患病率。由于现有的数据量,母鸡鸡蛋特别关注这一评估。在“阈值浓度”以下的水平下,没有发生鸡蛋蛋白衍生的过敏反应事件。日本的“阈值浓度”设定为每1克食品中含有10 μg的致敏蛋白。在实践方面,通过良好的卫生习惯,可以不断防止预包装食品中的过敏原污染。在日本食品工业中引入强制性的haccp方法有助于适当的控制,包括防止标签错误等。综上所述,根据现有证据,FSCJ认为日本现行的鸡蛋过敏原标识制度总体上是合适的。
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引用次数: 0
Acknowledgement for Reviewing. 审阅致谢。
Pub Date : 2021-12-24 eCollection Date: 2021-12-01 DOI: 10.14252/foodsafetyfscj.D-21-00027
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引用次数: 0
Current Status of Anisakiasis and Anisakis Larvae in Tokyo, Japan. 日本东京地区异尖线虫病及异尖线虫幼虫现状。
Pub Date : 2021-12-07 eCollection Date: 2021-12-01 DOI: 10.14252/foodsafetyfscj.D-21-00004
Jun Suzuki, Rie Murata, Yukihiro Kodo

Anisakiasis is a gastrointestinal disease caused by infection with anisakid nematodes. Anisakis larvae have been listed as distinct food poisoning agents in the manual of Food Poisoning Statistics, Japan since 2013. The reported numbers of food poisoning cases caused by Anisakis larvae are gradually increasing. A total of 94.0% of the causative larvae species were identified as Anisakis simplex sensu stricto (A. simplex), and 4.4% were identified as Anisakis pegreffii, among human-isolated anisakid nematodes examined in Tokyo Metropolitan Institute of Public Health, Japan from 2011 to 2018. Anisakis species infecting fishes in Japanese waters differ depending on their habitat and depth. A. simplex mainly infects fishes in the Pacific side of Japan, and A. pegreffii mainly infects fishes in the East China Sea and Sea of Japan sides. Regarding the causative foods of anisakiasis, cases by ingestion of mackerel (Scomber spp.) have been the most common in Japan, and cases caused by eating "marinated mackerel" accounted for 32.8% of the total in Tokyo from 2011 to 2017. However, the number of reports of food poisoning caused by skipjack tuna (Katsuwonus pelamis) was highest in May 2018 in Japan. A parasitological surveys of Anisakis third-stage larvae in skipjack tuna in Japanese waters were conducted in 2018 and 2019, and it was confirmed that more A. simplex infections of skipjack tuna may have occurred in 2018 than usual due to the meandering flow of the Black Current. Moreover, a portion of A. simplex larvae migrated from visceral organs to the ventral muscle in live skipjack tuna before capture, suggesting that an extensive cold chain after capture cannot prevent anisakiasis. In fish species that were reported to be high frequency of causative food of anisakiasis, it is necessary to freeze or at least remove the ventral muscle.

茴香线虫病是由茴香线虫感染引起的一种胃肠道疾病。自2013年起,日本《食物中毒统计手册》将异尖线虫幼虫列为不同的食物中毒物质。据报道,由异尖线虫幼虫引起的食物中毒病例正在逐渐增加。2011 - 2018年在日本东京都公共卫生研究所检测的人类分离的八角线虫中,94.0%的致病幼虫被鉴定为严格单形异尖线虫(A. simplex), 4.4%的致病幼虫被鉴定为pegreffii异尖线虫。感染日本水域鱼类的异尖线虫种类因其栖息地和深度而异。A. simplex主要感染日本太平洋一侧的鱼类,A. pegreffii主要感染东海和日本海一侧的鱼类。在致病食物方面,日本以食用鲭鱼(Scomber spp.)最为常见,2011 - 2017年东京因食用“腌制鲭鱼”引起的病例占总数的32.8%。然而,2018年5月在日本,由鲣鱼(Katsuwonus pelamis)引起的食物中毒报告数量最多。2018年和2019年在日本海域对鲣鱼第三期异尖线虫幼虫进行了寄生虫学调查,结果证实,由于黑流的蜿蜒流动,2018年鲣鱼的单形单尖线虫感染可能比平时更多。此外,在捕获活鲣鱼之前,部分单纯棘虫幼虫从内脏器官迁移到腹部肌肉,这表明捕获后的广泛冷链不能预防异丝蚴病。据报道,对易感染异烟线虫病的鱼类,有必要冷冻或至少切除其腹侧肌肉。
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引用次数: 9
Japanese Food Allergy-Labeling System and Comparison with the International Experience; Detection and Thresholds. 日本食品过敏标识制度及其与国际经验的比较检测和阈值。
Pub Date : 2021-12-07 eCollection Date: 2021-12-01 DOI: 10.14252/foodsafetyfscj.D-21-00008
Hiroshi Akiyama, Reiko Adachi

In the Japanese allergy-labeling system, food labeling is mandated for 7 specific ingredients (egg, cow's milk, wheat, buckwheat, peanut, shrimp, and crab) and recommended for 21 food ingredients in reference to case numbers of actual illness and the degree of seriousness. To monitor the validity of the labeling system, official methods for the detection of specific ingredient proteins in processed foods were developed. The official methods consist of ELISA methods for screening, and western blot methods for egg and milk, and PCR methods for wheat, buckwheat, peanut, shrimp/prawn, and crab as confirmation tests. The official methods consist of ELISA methods for screening, and western blot methods for egg and milk, and PCR methods for wheat, buckwheat, peanut, shrimp/prawn, and crab as confirmation tests. Threshold amounts (a few mg/kg) for labeling were set based on the approach of the analytical detections. Any foods containing protein allergens should be labeled if these contain allergens at greater than 10 ppm (mg/kg). Validation protocol criteria were established to standardize the Japanese official method. Food Safety Commission of Japan conducted a risk assessment of egg as a specific ingredient and judged that current labeling system for foods containing allergens is generally appropriate for "eggs". In the future, it is important to accumulate necessary scientific knowledge in order to carry out food health impact assessment including further refinement. The Japanese experience and knowledge of food allergy-labeling system would contribute to harmonize international labeling guidelines to protect allergic consumers globally.

在日本过敏标签制度中,对7种特定成分(鸡蛋、牛奶、小麦、荞麦、花生、虾、蟹)进行了强制性食品标签,并根据实际患病病例数和严重程度对21种食品成分进行了推荐。为了监测标签制度的有效性,制定了检测加工食品中特定成分蛋白质的官方方法。正式的方法包括用于筛选的ELISA方法和用于鸡蛋和牛奶的western blot方法,以及用于确认试验的小麦、荞麦、花生、虾/对虾和螃蟹的PCR方法。正式的方法包括用于筛选的ELISA方法和用于鸡蛋和牛奶的western blot方法,以及用于确认试验的小麦、荞麦、花生、虾/对虾和螃蟹的PCR方法。标签的阈值(几毫克/公斤)是根据分析检测的方法设定的。任何含有蛋白质过敏原的食物,如果其过敏原含量超过10ppm (mg/kg),都应贴上标签。建立验证方案标准,以规范日本官方方法。日本食品安全委员会对鸡蛋作为一种特定成分进行了风险评估,认为现行的含过敏原食品标签制度一般适用于“鸡蛋”。今后,为了开展食品健康影响评价,包括进一步的细化,需要积累必要的科学知识。日本在食品过敏标签制度方面的经验和知识将有助于协调国际标签准则,以保护全球过敏消费者。
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引用次数: 11
Recommended Practices to Eliminate Campylobacter from Live Birds and Chicken Meat in Japan. 在日本消除活禽和鸡肉中弯曲杆菌的建议做法。
Pub Date : 2021-09-24 eCollection Date: 2021-09-01 DOI: 10.14252/foodsafetyfscj.D-20-00021
Asano Kozu Clarke, Said Ajlouni

Campylobacter food poisoning is one of the major bacterial foodborne diseases resulting in numerous outbreaks worldwide. Particularly in Japan, one-fourth of the total food poisoning is caused by Campylobacter jejuni/coli. Raw and/or undercooked poultry meat and meat products are known as the main cause of campylobacteriosis. Consequently, effective and immediate actions are needed to eliminate or at least reduce campylobacteriosis. This study aimed at examining the Japanese food regulation system, comparing it with those in the USA and Australia, and making necessary recommendations for a better control of campylobacteriosis in Japan. The study was conducted by a thorough investigation of published literatures, governmental documents, statistical and epidemiological data and public information. The results led to recommendations that the Japanese food regulation authority should consider the following suggestions in order to control campylobacteriosis: 1) assess the Campylobacter safety at the end of processing stage of chicken supply chain based on risk assessment using quantitative/qualitative baseline data collected over Japan, 2) establish a national Campylobacter strategy, including specific campylobacteriosis reduction goals and criteria, and 3) provide the small food business operators with sufficient training and support to implement a Hazard Analysis Critical Control Points (HACCP) as an obligatory food safety requirement. It is acknowledged that it would be difficult to apply foreign regulations directly to Japanese food regulation system due to differences in food culture, regulation, industry structure, and data collection systems. Thus, flexible application is required. Finding and conducting effective Campylobacter control measures can decrease contaminated live birds and chicken meat in Japan, home to a unique food culture of eating raw and/or undercooked chicken meat called Torisashi such as sashimi, tataki and yubiki chicken. Consequently, potentially available research data may be instrumental in finding solutions for reducing campylobacteriosis. Eliminating Campylobacter food poisoning cases in Japan will be a significant achievement in ensuring Japanese and global food safety.

弯曲杆菌食物中毒是一种主要的细菌性食源性疾病,在世界范围内多次发生。特别是在日本,四分之一的食物中毒是由空肠弯曲杆菌/大肠杆菌引起的。生的和/或未煮熟的禽肉和肉制品被认为是弯曲杆菌病的主要原因。因此,需要立即采取有效的行动来消除或至少减少弯曲杆菌病。本研究旨在考察日本的食品监管体系,并与美国和澳大利亚的食品监管体系进行比较,为日本更好地控制弯曲杆菌病提出必要的建议。这项研究是通过对已发表的文献、政府文件、统计和流行病学数据以及公共信息进行全面调查进行的。研究结果建议日本食品监管机构应考虑以下建议,以控制弯曲杆菌病:1)基于在日本收集的定量/定性基线数据的风险评估,评估鸡肉供应链加工末端弯曲杆菌的安全性;2)建立国家弯曲杆菌战略,包括具体的弯曲杆菌减少目标和标准;3)为小型食品经营者提供足够的培训和支持,以实施危害分析关键控制点(HACCP)作为强制性食品安全要求。众所周知,由于饮食文化、法规、产业结构、数据收集系统等方面的差异,将外国法规直接适用于日本的食品监管体系存在一定的困难。因此,需要灵活的应用程序。在日本,发现并实施有效的弯曲杆菌控制措施可以减少受污染的活禽和鸡肉。日本有一种独特的饮食文化,即吃生的和/或未煮熟的鸡肉,如生鱼片、泷烧和鱼比鸡。因此,潜在可用的研究数据可能有助于找到减少弯曲杆菌病的解决方案。在日本消除弯曲杆菌食物中毒病例将是确保日本和全球食品安全的重大成就。
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引用次数: 2
Antimicrobial Drug-resistance Profile of Vibrio Parahaemolyticus isolated from Japanese Horse Mackerel (Trachurus Japonicus). 日本马鲛鱼副溶血性弧菌的耐药性分析。
Pub Date : 2021-09-24 eCollection Date: 2021-09-01 DOI: 10.14252/foodsafetyfscj.D-21-00001
Tasturo Nishino, Hideki Suzuki, Shiro Mizumoto, Hirotaka Morinushi, Hiromi Nagaoka, Keiichi Goto, Shigeki Yamamoto

This study aimed at investigating antimicrobial resistance (AMR) profile of Vibrio parahaemolyticus (V. parahaemolyticus). The bacteria were isolated from wild-caught and farmed Japanese horse mackerel (Trachurus japonicus), and examined for the antimicrobial drug resistance. Furthermore, the serotype, and the genes of thermostable direct hemolysin (tdh) and cholera toxin transcriptional activator (toxR) of the isolates were investigated by using a serotype testing kit and PCR method. Eighty-eight and 126 V. parahaemolyticus strains were isolated from wild-caught and farmed Japanese horse mackerel, respectively. Ten and 18 distinct serotypes were detected from wild-caught and farmed Japanese horse mackerel. All strains were negative for tdh genes but positive for toxR genes. Resistances to ampicillin (ABP) and to both ABP and fosfomycin (FOM) were observed in 54 and 23 strains from the wild-caught fish, while those resistant strains from farm fish were 112 and 7 strains. Multidrug-resistance to three or four drugs including ABP was observed in one or two strains from the wild-caught fish. These results strongly suggest that the environmental exposure of antimicrobial drugs results in the spread of resistant genes in Japanese horse mackerel. This study highlights the need for monitoring the spread of resistance genes to the human intestinal flora as well as to other bacteria in the environment.

本研究旨在研究副溶血性弧菌(V. parahaemolyticus)的抗微生物药物耐药性(AMR)。从野生和养殖的日本马鲛鱼(Trachurus japonicus)中分离出细菌,并进行了抗菌药物耐药性检测。此外,采用血清型检测试剂盒和PCR方法对分离株进行了血清型检测,并对热稳定型直接溶血素(tdh)和霍乱毒素转录激活因子(toxR)基因进行了检测。从野生马鲛鱼和养殖马鲛鱼中分别分离到88株和126株副溶血性弧菌。从野生和养殖的日本马鲛鱼中分别检测到10种和18种不同的血清型。所有菌株tdh基因均阴性,而toxR基因均阳性。对氨苄西林(ABP)和磷霉素(FOM)均耐药的野生鱼有54株和23株,对养殖鱼有112株和7株。在野生捕获的鱼类中发现1 ~ 2株对包括ABP在内的3 ~ 4种药物具有多重耐药。这些结果有力地表明,抗菌药的环境暴露导致了日本马鲛鱼耐药基因的传播。这项研究强调了监测耐药基因在人类肠道菌群以及环境中其他细菌中的传播的必要性。
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引用次数: 2
On the Campylobacter Papers in this Issue. 本期关于弯曲杆菌的论文。
Pub Date : 2021-09-24 eCollection Date: 2021-09-01 DOI: 10.14252/foodsafetyfscj.D-21-00014
Shigeki Yamamoto
Abstract
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引用次数: 0
Development and Evaluation of Fluorescence Immunochromatography for Rapid and Sensitive Detection of Thermophilic Campylobacter. 开发和评估用于快速灵敏检测嗜热弯曲杆菌的荧光免疫层析技术。
Pub Date : 2021-09-24 eCollection Date: 2021-09-01 DOI: 10.14252/foodsafetyfscj.D-21-00006
Hiroshi Asakura, Junko Sakata, Yoshimasa Sasaki, Kentaro Kawatsu

Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli) are leading causes of foodborne gastroenteritis in Japan. Epidemiological surveillance has provided evidence that poultry meat is one of the main reservoirs for human campylobacteriosis, and therefore, improvement in process hygiene at slaughter is required to reduce the number of human infections. This study thus aimed to develop fluorescent immunochromatography strips for rapid and sensitive detection of thermophilic Campylobacter on poultry carcasses at slaughter. To establish the required detection levels, we first determined the numbers of C. jejuni and C. coli on poultry carcasses at one large-scale poultry slaughterhouse in Japan, resulting in the detection of Campylobacter at 1.97 ± 0.24 log CFU/25 g of neck skin during the post-chilling process by using ISO 10272-2:2017. Our developed Campylobacter fluorescence immunochromatography (FIC) assay exhibited a 50% limit of detection of 3.51 log CFU or 4.34 log CFU for C. jejuni NCTC 11168 or C. coli JCM 2529, respectively. Inclusive and exclusive tests resulted in good agreement. The practical usefulness of this test toward poultry carcasses should be evaluated in future studies, perhaps concentration of the target microorganisms prior to the testing might be helpful to further enhance sensitivity. Nevertheless, our data suggest the potential of FIC for rapid and sensitive detection of thermophilic Campylobacter for monitoring the process hygiene of poultry carcasses at slaughter.

空肠弯曲菌(C. jejuni)和大肠弯曲菌(C. coli)是日本食源性肠胃炎的主要病因。流行病学监测表明,禽肉是人类弯曲杆菌病的主要病原之一,因此需要改善屠宰过程的卫生状况,以减少人类感染的数量。因此,本研究旨在开发荧光免疫层析条,用于快速灵敏地检测屠宰家禽胴体上的嗜热弯曲杆菌。为了确定所需的检测水平,我们首先测定了日本一家大型家禽屠宰场的家禽胴体上空肠弯曲菌和大肠弯曲菌的数量,结果通过 ISO 10272-2:2017,在后冷却过程中检测到弯曲菌的数量为 1.97 ± 0.24 log CFU/25 g 颈部皮肤。我们开发的弯曲菌荧光免疫层析(FIC)检测法对空肠弯曲菌 NCTC 11168 或大肠弯曲菌 JCM 2529 的 50% 检出限分别为 3.51 log CFU 或 4.34 log CFU。包容性和排他性测试结果一致。这种检测方法对家禽胴体的实用性应在今后的研究中进行评估,也许在检测前浓缩目标微生物会有助于进一步提高灵敏度。不过,我们的数据表明,FIC 具有快速、灵敏地检测嗜热弯曲菌的潜力,可用于监控屠宰家禽胴体的加工卫生。
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引用次数: 0
期刊
Food safety (Tokyo, Japan)
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