Washington State's suicide rate is 11% higher than the national average, making it one of the top 10 states with the highest suicide rates in the U.S.. Although efforts have been made to address behavioral factors that contribute to suicide risk, little attention has been given to the impact of socioeconomic factors on the mental health of Washington residents. The purpose of the research is to investigate the relationship between socioeconomic status and suicide rates in Washington State. The results of the study can help guide policymakers and healthcare professionals in developing intervention strategies to reduce suicide risk. The suicide rate among Washington residents from 2011-2020 was provided by the 57 vital statistics jurisdictions participating in the Vital Statistics Cooperative Program. Three indicators, including poverty rate, lower than high school graduate rate, and unemployment rate, were chosen to explore the relationship between socioeconomic factors and suicide rate. The data from 57 jurisdictions were analyzed using R, with F tests and multiple linear regressions employed. The multiple R-squared value was 0.7648, indicating that 76.48% of the variation can be explained by the independent variables. These results suggest that poverty and employment rates have a significant positive relationship with suicide rate. However, the relationship between lower educational attainment and suicide rate was not statistically significant. Addressing the issue of rising suicide rates requires a collaborative effort between healthcare officials and policymakers. Policymakers should prioritize promoting job opportunities and providing educational resources to alleviate financial stress and increase access to mental healthcare.
{"title":"Socioeconomic Factors and their Influence on Suicide rate: a Multiple Regression case Study in Washington","authors":"Zixuan Wu, Yueqian Zhang","doi":"10.26502/fjhs.140","DOIUrl":"https://doi.org/10.26502/fjhs.140","url":null,"abstract":"Washington State's suicide rate is 11% higher than the national average, making it one of the top 10 states with the highest suicide rates in the U.S.. Although efforts have been made to address behavioral factors that contribute to suicide risk, little attention has been given to the impact of socioeconomic factors on the mental health of Washington residents. The purpose of the research is to investigate the relationship between socioeconomic status and suicide rates in Washington State. The results of the study can help guide policymakers and healthcare professionals in developing intervention strategies to reduce suicide risk. The suicide rate among Washington residents from 2011-2020 was provided by the 57 vital statistics jurisdictions participating in the Vital Statistics Cooperative Program. Three indicators, including poverty rate, lower than high school graduate rate, and unemployment rate, were chosen to explore the relationship between socioeconomic factors and suicide rate. The data from 57 jurisdictions were analyzed using R, with F tests and multiple linear regressions employed. The multiple R-squared value was 0.7648, indicating that 76.48% of the variation can be explained by the independent variables. These results suggest that poverty and employment rates have a significant positive relationship with suicide rate. However, the relationship between lower educational attainment and suicide rate was not statistically significant. Addressing the issue of rising suicide rates requires a collaborative effort between healthcare officials and policymakers. Policymakers should prioritize promoting job opportunities and providing educational resources to alleviate financial stress and increase access to mental healthcare.","PeriodicalId":73052,"journal":{"name":"Fortune journal of health sciences","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135211789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Invasive fungal infections (IFI) are common nosocomial infections in immunosuppressed individuals. Rapid diagnosis of IFIs is important to support the growing number of at-risk patients and standardize the treatment guidelines. The study aimed to assess the role of serum 1,3-β-D-Glucan (BDG) and galactomannan (GM) biomarkers and real-time PCR in the rapid diagnosis of IFIs.
侵袭性真菌感染(IFI)是免疫抑制个体中常见的医院感染。ifi的快速诊断对于支持越来越多的高危患者和使治疗指南标准化具有重要意义。本研究旨在评估血清1,3-β- d -葡聚糖(BDG)和半乳甘露聚糖(GM)生物标志物和实时PCR在IFIs快速诊断中的作用。
{"title":"Rapid Diagnosis of Invasive Fungal Infections Caused by Candida and Aspergillus Species in Patients Admitted to Intensive Care Unit of a Tertiary Care Hospital","authors":"Shaila Akhtar, Shaheda Anwar, Ahmed Abu Saleh","doi":"10.26502/fjhs.138","DOIUrl":"https://doi.org/10.26502/fjhs.138","url":null,"abstract":"Introduction: Invasive fungal infections (IFI) are common nosocomial infections in immunosuppressed individuals. Rapid diagnosis of IFIs is important to support the growing number of at-risk patients and standardize the treatment guidelines. The study aimed to assess the role of serum 1,3-β-D-Glucan (BDG) and galactomannan (GM) biomarkers and real-time PCR in the rapid diagnosis of IFIs.","PeriodicalId":73052,"journal":{"name":"Fortune journal of health sciences","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135101358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01Epub Date: 2023-09-27DOI: 10.26502/fjhs.134
Jia-Hua Qu, Kirill V Tarasov, Yelena S Tarasova, Khalid Chakir, Edward G Lakatta
Advancing age is the most important risk factor for cardiovascular diseases (CVDs). Two types of cells, within the heart pacemaker, sinoatrial node (SAN), and within the left ventricle (LV), control two crucial characteristics of heart function, heart beat rate and contraction strength. As age advances, the heart's structure becomes remodeled, and SAN and LV cell functions deteriorate, thus increasing the risk for CVDs. However, the different molecular features of age-associated changes in SAN and LV cells have never been compared in omics scale in the context of aging. We applied deep RNA sequencing to four groups of samples, young LV, old LV, young SAN and old SAN, followed by numerous bioinformatic analyses. In addition to profiling the differences in gene expression patterns between the two heart chambers (LV vs. SAN), we also identified the chamber-specific concordant or discordant age-associated changes in: (1) genes linked to energy production related to cardiomyocyte contraction, (2) genes related to post-transcriptional processing, (3) genes involved in KEGG longevity regulating pathway, (4) prolongevity and antilongevity genes recorded and curated in the GenAge database, and (5) CVD marker genes. Our bioinformatic analysis also predicted the regulation activities and mapped the expression of upstream regulators including transcription regulators and post-transcriptional regulator miRNAs. This comprehensive analysis promotes our understanding of regulation of heart functions and will enable discovery of gene-specific therapeutic targets of CVDs in advanced age.
{"title":"Transcriptome of Left Ventricle and Sinoatrial Node in Young and Old C57 Mice.","authors":"Jia-Hua Qu, Kirill V Tarasov, Yelena S Tarasova, Khalid Chakir, Edward G Lakatta","doi":"10.26502/fjhs.134","DOIUrl":"10.26502/fjhs.134","url":null,"abstract":"<p><p>Advancing age is the most important risk factor for cardiovascular diseases (CVDs). Two types of cells, within the heart pacemaker, sinoatrial node (SAN), and within the left ventricle (LV), control two crucial characteristics of heart function, heart beat rate and contraction strength. As age advances, the heart's structure becomes remodeled, and SAN and LV cell functions deteriorate, thus increasing the risk for CVDs. However, the different molecular features of age-associated changes in SAN and LV cells have never been compared in omics scale in the context of aging. We applied deep RNA sequencing to four groups of samples, young LV, old LV, young SAN and old SAN, followed by numerous bioinformatic analyses. In addition to profiling the differences in gene expression patterns between the two heart chambers (LV vs. SAN), we also identified the chamber-specific concordant or discordant age-associated changes in: (1) genes linked to energy production related to cardiomyocyte contraction, (2) genes related to post-transcriptional processing, (3) genes involved in KEGG longevity regulating pathway, (4) prolongevity and antilongevity genes recorded and curated in the GenAge database, and (5) CVD marker genes. Our bioinformatic analysis also predicted the regulation activities and mapped the expression of upstream regulators including transcription regulators and post-transcriptional regulator miRNAs. This comprehensive analysis promotes our understanding of regulation of heart functions and will enable discovery of gene-specific therapeutic targets of CVDs in advanced age.</p>","PeriodicalId":73052,"journal":{"name":"Fortune journal of health sciences","volume":"6 3","pages":"332-356"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10621664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71429765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jasreen Kaur Sandhu, Gurkaranvir Singh, S. Batth, Navjot Kaur
Granulomatosis with polyangiitis (GPA), a type of vasculitis, is characterized by necrotizing inflammation of small-medium blood vessels and is associated with anti-neutrophil cytoplasmic antibodies (ANCA). While GPA commonly affects the upper respiratory tract, lungs, and kidneys, ocular manifestations can also occur as a part of systemic GPA or isolated GPA involving eyes, the diagnosis of which can be challenging. Ocular involvement in GPA is variable and can present as scleritis, keratitis, conjunctivitis, uveitis, or orbital involvement. This review article briefly explores the etiology of GPA with a focus on the ophthalmic manifestations of GPA, including their clinical features, diagnosis, differentials, and treatment options. Understanding the ocular manifestations can help in early diagnosis, preventing irreversible eye damage and vision loss. Conclusion: The small blood vessels in almost any organ or tissue can be involved in GPA, the involvement of the eye being one such organ which can present with non-specific signs and symptoms in both generalized and isolated forms. Knowledge of the ocular manifestations of GPA can help catch the disease in its initial stage and prevent irreversible eye damage. Despite being recognized in over half of patients with GPA, ophthalmic signs go misdiagnosed in most patients. The most efficacious strategy for preventing disease mortality and morbidity is having a high level of clinical suspicion, early diagnosis, and commencing immunosuppressive medication early in the disease course.
{"title":"Ophthalmic Manifestations in Granulomatosis with Polyangiitis: A Narrative Review","authors":"Jasreen Kaur Sandhu, Gurkaranvir Singh, S. Batth, Navjot Kaur","doi":"10.26502/fjhs.130","DOIUrl":"https://doi.org/10.26502/fjhs.130","url":null,"abstract":"Granulomatosis with polyangiitis (GPA), a type of vasculitis, is characterized by necrotizing inflammation of small-medium blood vessels and is associated with anti-neutrophil cytoplasmic antibodies (ANCA). While GPA commonly affects the upper respiratory tract, lungs, and kidneys, ocular manifestations can also occur as a part of systemic GPA or isolated GPA involving eyes, the diagnosis of which can be challenging. Ocular involvement in GPA is variable and can present as scleritis, keratitis, conjunctivitis, uveitis, or orbital involvement. This review article briefly explores the etiology of GPA with a focus on the ophthalmic manifestations of GPA, including their clinical features, diagnosis, differentials, and treatment options. Understanding the ocular manifestations can help in early diagnosis, preventing irreversible eye damage and vision loss. Conclusion: The small blood vessels in almost any organ or tissue can be involved in GPA, the involvement of the eye being one such organ which can present with non-specific signs and symptoms in both generalized and isolated forms. Knowledge of the ocular manifestations of GPA can help catch the disease in its initial stage and prevent irreversible eye damage. Despite being recognized in over half of patients with GPA, ophthalmic signs go misdiagnosed in most patients. The most efficacious strategy for preventing disease mortality and morbidity is having a high level of clinical suspicion, early diagnosis, and commencing immunosuppressive medication early in the disease course.","PeriodicalId":73052,"journal":{"name":"Fortune journal of health sciences","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69347699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Bronchial asthma (BA) and thyroid nodules (TNs) are growing global health challenges. The present study thus aimed to investigate the prevalence of BA and its associated risk factors among adults with TNs in the eastern region of the Kingdom of Saudi Arabia (KSA).
{"title":"Bronchial Asthma and Its Associated Risk Factors Among Adults with Thyroid Nodules","authors":"Imad R Musa","doi":"10.26502/fjhs.144","DOIUrl":"https://doi.org/10.26502/fjhs.144","url":null,"abstract":"Background: Bronchial asthma (BA) and thyroid nodules (TNs) are growing global health challenges. The present study thus aimed to investigate the prevalence of BA and its associated risk factors among adults with TNs in the eastern region of the Kingdom of Saudi Arabia (KSA).","PeriodicalId":73052,"journal":{"name":"Fortune journal of health sciences","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135507265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SARS-CoV-2 that caused the COVID-19 pandemic is one exceptional virus with its high transmissivity and delayed onset of symptoms. This article describes the efforts put forth by the biotech industry and universities in Singapore to develop diagnostic tests that aid the detection of positive cases. Direct tests such as RT-PCR and antigen rapid test profile the virus nucleic acid and viral proteins, respectively. But, of equal importance in case detection and treatment are serological tests that measure the relative abundance of IgM and IgG which is indicative of infection phase and quality of immune response in positive cases. Other tests such as isothermal amplification, CRISPR-based diagnostics and breath tests are also in development or in emergency use and would undoubtedly provide valuable usage experience important for the development of molecular assays to detect the next pathogen of global concern.
{"title":"Technological Innovations in COVID-19 Diagnostics in Singapore","authors":"Wenfa Ng","doi":"10.26502/fjhs.127","DOIUrl":"https://doi.org/10.26502/fjhs.127","url":null,"abstract":"SARS-CoV-2 that caused the COVID-19 pandemic is one exceptional virus with its high transmissivity and delayed onset of symptoms. This article describes the efforts put forth by the biotech industry and universities in Singapore to develop diagnostic tests that aid the detection of positive cases. Direct tests such as RT-PCR and antigen rapid test profile the virus nucleic acid and viral proteins, respectively. But, of equal importance in case detection and treatment are serological tests that measure the relative abundance of IgM and IgG which is indicative of infection phase and quality of immune response in positive cases. Other tests such as isothermal amplification, CRISPR-based diagnostics and breath tests are also in development or in emergency use and would undoubtedly provide valuable usage experience important for the development of molecular assays to detect the next pathogen of global concern.","PeriodicalId":73052,"journal":{"name":"Fortune journal of health sciences","volume":"255 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136160125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01Epub Date: 2023-04-19DOI: 10.26502/fjhs.112
Sophiya Karki, Weijing Sun, Rashna Madan, Kamal Lamsal, Sarah Schmitt, Andrew K Godwin, Anup Kasi
Colorectal cancer (CRC) has tremendous molecular and genetic heterogeneity, making it a difficult cancer to treat. Two of the key prognostic indicators of CRC include microsatellite instability (MSI) and BRAF V600E mutation. Here, we performed a retrospective survival analysis on 145 stage II and III CRC patients treated at the University of Kansas Cancer Center between 2009 and 2020. Of the 145 patients, BRAF V600E was observed in 15% patients and MSI in 28% patients. Median survival was not reached for stage II. For stage III, patients with BRAF V600E showed poor overall survival, which worsened with concurrent presence of MSI [χ2=6.4, p=0.01]. Eighty-five percent of this group was found to have right-sided CRC. For stage III, overall survival (OS) was 27 months, 37 months, 87 months and not reached for MSI-H/BRAF V600E, MSS/BRAF V600E, MSS/BRAF WT and MSI-H/BRAF WT, respectively. Although associated with poor prognosis, presence of MSI in BRAF V600E patients was associated with delayed disease presentation (mean age 77) compared to those with stable microsatellite (mean age 63) [p=0.01]. Although median survival between the groups could not be assessed for stage II due to very few deaths and/or inadequate length of study, comparison of survival trend suggests that BRAF V600E, rather than MSI, is what drives prognosis in stage II CRC. Our findings suggest that prognostic value of MSI is more relevant for stage III than stage II CRC. Patients with MSI-H and BRAF V600E have advantage of late presentation, although at the cost of poor overall prognosis.
{"title":"Microsatellite Instability with BRAF V600E Associated with Delayed Presentation but Poor Survival in Stage III Colorectal Cancer.","authors":"Sophiya Karki, Weijing Sun, Rashna Madan, Kamal Lamsal, Sarah Schmitt, Andrew K Godwin, Anup Kasi","doi":"10.26502/fjhs.112","DOIUrl":"https://doi.org/10.26502/fjhs.112","url":null,"abstract":"Colorectal cancer (CRC) has tremendous molecular and genetic heterogeneity, making it a difficult cancer to treat. Two of the key prognostic indicators of CRC include microsatellite instability (MSI) and BRAF V600E mutation. Here, we performed a retrospective survival analysis on 145 stage II and III CRC patients treated at the University of Kansas Cancer Center between 2009 and 2020. Of the 145 patients, BRAF V600E was observed in 15% patients and MSI in 28% patients. Median survival was not reached for stage II. For stage III, patients with BRAF V600E showed poor overall survival, which worsened with concurrent presence of MSI [χ2=6.4, p=0.01]. Eighty-five percent of this group was found to have right-sided CRC. For stage III, overall survival (OS) was 27 months, 37 months, 87 months and not reached for MSI-H/BRAF V600E, MSS/BRAF V600E, MSS/BRAF WT and MSI-H/BRAF WT, respectively. Although associated with poor prognosis, presence of MSI in BRAF V600E patients was associated with delayed disease presentation (mean age 77) compared to those with stable microsatellite (mean age 63) [p=0.01]. Although median survival between the groups could not be assessed for stage II due to very few deaths and/or inadequate length of study, comparison of survival trend suggests that BRAF V600E, rather than MSI, is what drives prognosis in stage II CRC. Our findings suggest that prognostic value of MSI is more relevant for stage III than stage II CRC. Patients with MSI-H and BRAF V600E have advantage of late presentation, although at the cost of poor overall prognosis.","PeriodicalId":73052,"journal":{"name":"Fortune journal of health sciences","volume":"6 2","pages":"167-173"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/36/92/nihms-1896281.PMC10512748.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41166930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A New Format for the Italian National Health System: from Hospital to Patient-Centric Care","authors":"Matteo Maria Cati","doi":"10.26502/fjhs.124","DOIUrl":"https://doi.org/10.26502/fjhs.124","url":null,"abstract":"","PeriodicalId":73052,"journal":{"name":"Fortune journal of health sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69347599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Lagaré, Zaliha A. Lahama, Fatima Hassane, Wilfried Hounkanrin, H. Yayé
{"title":"Prevalence and risk of Propagation of Covid-19 Infection among Prisoners in Niamey-Niger","authors":"A. Lagaré, Zaliha A. Lahama, Fatima Hassane, Wilfried Hounkanrin, H. Yayé","doi":"10.26502/fjhs.125","DOIUrl":"https://doi.org/10.26502/fjhs.125","url":null,"abstract":"","PeriodicalId":73052,"journal":{"name":"Fortune journal of health sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69347646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Albert Oueremi, A. Ouattara, A. M. Dabiré, Rahimatou Yasmine Wendkuni Tiemtoré, Serge Sougué, Jacques Simpor
Background: I nfections caused by biofilm-producing microbes are associated with common human illnesses that are difficult to treat due to antibiotic resistance, especially when the bacteria also produce beta-lactamases. The aim of the present study was to evaluate the ability of clinical strains of Escherichia coli and Pseudomonas spp that produce beta-lactamases to produce biofilm. Methods: The study involved two clinical strains of Escherichia coli and Pseudomonas spp that produce beta-lactamases and were isolated from pus samples at the Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle of Ouagadougou, Burkina Faso. Biofilm production was assessed using the microtiter plate-based crystal violet assay, with the PA01 WT strain used as a positive control for biofilm production. Biofilm was quantified by measuring optical densities with a spectrophotometer. Results: The E. coli strain was resistant to cefotaxime, ceftriaxone, and ceftazidime, while in addition to these antibiotics, Pseudomonas spp was resistant to imipenem. Both strains were beta-lactamase producers confirmed by the detection of bla NDM and bla IMP genes in E. coli and bla NDM , bla CTX and bla SHV in Pseudomonas spp . Optical density measurements after crystal violet staining showed that both strains were moderate biofilm producers. Conclusions: This study highlights that clinical isolates of E. coli and Pseudomonas spp , which are responsible for human infections and produce beta-lactamases, are also moderate biofilm producers. This is a real public health concern requiring surveillance efforts and investigations to prevent and effectively combat this form of resistance.
{"title":"Moderate Production of Biofilm by Clinical isolates of E. coli and Pseudomonas spp in Burkina Faso","authors":"Albert Oueremi, A. Ouattara, A. M. Dabiré, Rahimatou Yasmine Wendkuni Tiemtoré, Serge Sougué, Jacques Simpor","doi":"10.26502/fjhs.129","DOIUrl":"https://doi.org/10.26502/fjhs.129","url":null,"abstract":"Background: I nfections caused by biofilm-producing microbes are associated with common human illnesses that are difficult to treat due to antibiotic resistance, especially when the bacteria also produce beta-lactamases. The aim of the present study was to evaluate the ability of clinical strains of Escherichia coli and Pseudomonas spp that produce beta-lactamases to produce biofilm. Methods: The study involved two clinical strains of Escherichia coli and Pseudomonas spp that produce beta-lactamases and were isolated from pus samples at the Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle of Ouagadougou, Burkina Faso. Biofilm production was assessed using the microtiter plate-based crystal violet assay, with the PA01 WT strain used as a positive control for biofilm production. Biofilm was quantified by measuring optical densities with a spectrophotometer. Results: The E. coli strain was resistant to cefotaxime, ceftriaxone, and ceftazidime, while in addition to these antibiotics, Pseudomonas spp was resistant to imipenem. Both strains were beta-lactamase producers confirmed by the detection of bla NDM and bla IMP genes in E. coli and bla NDM , bla CTX and bla SHV in Pseudomonas spp . Optical density measurements after crystal violet staining showed that both strains were moderate biofilm producers. Conclusions: This study highlights that clinical isolates of E. coli and Pseudomonas spp , which are responsible for human infections and produce beta-lactamases, are also moderate biofilm producers. This is a real public health concern requiring surveillance efforts and investigations to prevent and effectively combat this form of resistance.","PeriodicalId":73052,"journal":{"name":"Fortune journal of health sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69347664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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