Pub Date : 2017-12-29DOI: 10.21010/AJTCAM.VI15.1.9
H. E. Gbekley, S. Karou, G. Katawa, T. Tchacondo, K. Batawila, Y. Ameyapoh, J. Simporé
Background: Hypertension is one of the most common chronic diseases in modern societies and there is evidence that its incidence and severity are increasing. This survey was conducted to investigate the plants used by the Togolese traditional healers to treat the disease. Method: From January to June 2016, an ethnobotanical survey was conducted using a semi-structured questionnaire with traditional healers (TH) in the southern region of Togo. The importance of the plants was assessed by the calculation of the use value (UV). Results: In Total, 128 TH male and female were interviewed and 116 plants species belonging to 46 families were identified as treating hypertension. The most represented families were: Fabaceae with 16 species followed by Euphorbiaceae and Rutaceae contributing with 8 and 6 species respectively. Based on the calculated use values the most important species were Byrsocarpus coccineus Schum. Et thonn. (UV = 0.47); Crateva religiosa G.Forst. (UV = 0.47), Boerhavia diffusa Engelm. &A.Gray L. (UV = 0.47), Xylopia aethiopica A. Rich. (UV = 0.42), Mangnifera indica L. (UV = 0.38). The leaves and the roots were the parts of plant predominantly used to prepare the recipes, mainly decoctions administrated by oral route. Clinical manifestations such as dizzy spells, swarming, loss of consciousness, severe headache, severe anxiety and shortness of breath, nosebleed, and fear of heights were used by TH to diagnose the disease. Conclusion: This study showed initial evidence of the use of plant materials by Togolese TH to treat hypertension. These results constitute a database for pharmacological screenings with the aim of developing new therapies.
{"title":"ETHNOBOTANICAL SURVEY OF MEDICINAL PLANTS USED IN THE MANAGEMENT OF HYPERTENSION IN THE MARITIME REGION OF TOGO","authors":"H. E. Gbekley, S. Karou, G. Katawa, T. Tchacondo, K. Batawila, Y. Ameyapoh, J. Simporé","doi":"10.21010/AJTCAM.VI15.1.9","DOIUrl":"https://doi.org/10.21010/AJTCAM.VI15.1.9","url":null,"abstract":"Background: Hypertension is one of the most common chronic diseases in modern societies and there is evidence that its incidence and severity are increasing. This survey was conducted to investigate the plants used by the Togolese traditional healers to treat the disease. \u0000Method: From January to June 2016, an ethnobotanical survey was conducted using a semi-structured questionnaire with traditional healers (TH) in the southern region of Togo. The importance of the plants was assessed by the calculation of the use value (UV). \u0000Results: In Total, 128 TH male and female were interviewed and 116 plants species belonging to 46 families were identified as treating hypertension. The most represented families were: Fabaceae with 16 species followed by Euphorbiaceae and Rutaceae contributing with 8 and 6 species respectively. Based on the calculated use values the most important species were Byrsocarpus coccineus Schum. Et thonn. (UV = 0.47); Crateva religiosa G.Forst. (UV = 0.47), Boerhavia diffusa Engelm. &A.Gray L. (UV = 0.47), Xylopia aethiopica A. Rich. (UV = 0.42), Mangnifera indica L. (UV = 0.38). The leaves and the roots were the parts of plant predominantly used to prepare the recipes, mainly decoctions administrated by oral route. Clinical manifestations such as dizzy spells, swarming, loss of consciousness, severe headache, severe anxiety and shortness of breath, nosebleed, and fear of heights were used by TH to diagnose the disease. \u0000Conclusion: This study showed initial evidence of the use of plant materials by Togolese TH to treat hypertension. These results constitute a database for pharmacological screenings with the aim of developing new therapies.","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"66 1","pages":"85-97"},"PeriodicalIF":0.0,"publicationDate":"2017-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89015239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-29DOI: 10.21010/AJTCAM.VI15.1.7
Gabriel O. Anyanwua, C. E. Onyeneke, P. Ofoha, K. Rauf, Usunomena Usunobun, Nisar ur-Rehman
Background : Anthocleista vogelii Planch is a medicinal plant used by traditional healers in the treatment of Malaria, hypertension, ulcer, obesity, diabetes, and as a diuretic and purgative in Africa. Therefore, the present study sought to evaluate the diuretic, antidiuretic and laxative activities of the root bark of A. vogelii extracts and fractions in male Sprague-Dawley rats. Materials and methods : Sixty rats were placed in 10 groups which included: control (normal saline), furosemide/sodium picosulfate (25 mg/kg) as standard drugs, methanol extracts (250 mg/kg and 500 mg/kg), and 125 mg/kg and 250 mg/kg of saponin, flavonoid and neutral alkaloid fractions of A. vogelii . The drugs/extracts/fractions were administered orally using normal saline as the vehicle. Results : The 500 mg/kg methanol extracts (ME) significantly increased (p<0.05) the urine volume and electrolytes (Na + , K + and Cl - ) excreted, while 125 mg/kg and 250 mg/kg flavonoid fraction (FF) decreased significantly (p<0.05) urine volume and electrolytes (Na + , K + and Cl - ) excreted, but doses of saponin fraction (SF) and neutral alkaloid fraction (NAF) had no significant difference (p<0.05) compared to the control after 5 hrs. ME, SF, NAF significantly increased (p<0.05) the fecal output of the animals when compared to the control, while FF showed no significant difference (p<0.05) after 8 hrs of administration. Conclusions : This study determined that ME revealed diuretic activity, although not remarkable to furosemide, while FF showed antidiuretic activity, and potent laxative activities were discovered in ME and SF of A. vogelii . Keywords : Anthocleista vogelii , Diuretic, Anti-Diuretic, Laxative, Traditional healers
{"title":"DIURETIC, ANTIDIURETIC AND LAXATIVE ACTIVITIES OF ANTHOCLEISTA VOGELII EXTRACTS IN RATS","authors":"Gabriel O. Anyanwua, C. E. Onyeneke, P. Ofoha, K. Rauf, Usunomena Usunobun, Nisar ur-Rehman","doi":"10.21010/AJTCAM.VI15.1.7","DOIUrl":"https://doi.org/10.21010/AJTCAM.VI15.1.7","url":null,"abstract":"Background : Anthocleista vogelii Planch is a medicinal plant used by traditional healers in the treatment of Malaria, hypertension, ulcer, obesity, diabetes, and as a diuretic and purgative in Africa. Therefore, the present study sought to evaluate the diuretic, antidiuretic and laxative activities of the root bark of A. vogelii extracts and fractions in male Sprague-Dawley rats. Materials and methods : Sixty rats were placed in 10 groups which included: control (normal saline), furosemide/sodium picosulfate (25 mg/kg) as standard drugs, methanol extracts (250 mg/kg and 500 mg/kg), and 125 mg/kg and 250 mg/kg of saponin, flavonoid and neutral alkaloid fractions of A. vogelii . The drugs/extracts/fractions were administered orally using normal saline as the vehicle. Results : The 500 mg/kg methanol extracts (ME) significantly increased (p<0.05) the urine volume and electrolytes (Na + , K + and Cl - ) excreted, while 125 mg/kg and 250 mg/kg flavonoid fraction (FF) decreased significantly (p<0.05) urine volume and electrolytes (Na + , K + and Cl - ) excreted, but doses of saponin fraction (SF) and neutral alkaloid fraction (NAF) had no significant difference (p<0.05) compared to the control after 5 hrs. ME, SF, NAF significantly increased (p<0.05) the fecal output of the animals when compared to the control, while FF showed no significant difference (p<0.05) after 8 hrs of administration. Conclusions : This study determined that ME revealed diuretic activity, although not remarkable to furosemide, while FF showed antidiuretic activity, and potent laxative activities were discovered in ME and SF of A. vogelii . Keywords : Anthocleista vogelii , Diuretic, Anti-Diuretic, Laxative, Traditional healers","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"27 1","pages":"66-73"},"PeriodicalIF":0.0,"publicationDate":"2017-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82839364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-29DOI: 10.21010/AJTCAM.VI15.1.10
Huanlin Huang, Zheng Zhong, Xinyi Zhang, Jizhou Wang, Yujie Li, O. B. Fasmer, G. Wik, Yong Huang, Shaohui Huang
Background: Fear is one of the most widely studied emotions and is closely associated with the autonomic nervous system (ANS). Previous studies have proven that acupuncture directly impacts the ANS, influences the heart rate (HR) and the heart rate variability (HRV) and exerts other effects. The aim of this study was to explore the effect of Jianshi (PC5) and Neiguan (PC6) electro-acupuncture on HRV during fear-invoking auditory stimulation using an Actiheart ECG recorder. Materials and Methods: Two hundred healthy subjects were recruited. Using a random number table, subjects were grouped for exposure to fear-invoking auditory stimulation (n=40) or neutral auditory stimulation (n=40). After determining that our fear-invoking auditory stimulation produced the fear emotion, the other 120 subjects were similarly divided into an electro-acupuncture (EA group) and a control group that received PC5 and PC6 electro-acupuncture or no intervention. Results: The fear score of the fear-invoking auditory group was significantly higher than that of the neutral auditory group. The EA group showed higher SD, RMSSD, and high frequency (HF) components of HRV than those of the control group. Conclusion: The primary result suggests that PC5 and PC6 electro-acupuncture affects cardiac autonomic neural regulation, mainly via the parasympathetic system, in subjects exposed to fear-invoking auditory stimulation.
{"title":"Electro-acupuncture at Jianshi (PC5) and Neiguan (PC6) alters heart rate variability (HRV) in frightened volunteers","authors":"Huanlin Huang, Zheng Zhong, Xinyi Zhang, Jizhou Wang, Yujie Li, O. B. Fasmer, G. Wik, Yong Huang, Shaohui Huang","doi":"10.21010/AJTCAM.VI15.1.10","DOIUrl":"https://doi.org/10.21010/AJTCAM.VI15.1.10","url":null,"abstract":"Background: Fear is one of the most widely studied emotions and is closely associated with the autonomic nervous system (ANS). Previous studies have proven that acupuncture directly impacts the ANS, influences the heart rate (HR) and the heart rate variability (HRV) and exerts other effects. The aim of this study was to explore the effect of Jianshi (PC5) and Neiguan (PC6) electro-acupuncture on HRV during fear-invoking auditory stimulation using an Actiheart ECG recorder. \u0000Materials and Methods: Two hundred healthy subjects were recruited. Using a random number table, subjects were grouped for exposure to fear-invoking auditory stimulation (n=40) or neutral auditory stimulation (n=40). After determining that our fear-invoking auditory stimulation produced the fear emotion, the other 120 subjects were similarly divided into an electro-acupuncture (EA group) and a control group that received PC5 and PC6 electro-acupuncture or no intervention. \u0000Results: The fear score of the fear-invoking auditory group was significantly higher than that of the neutral auditory group. The EA group showed higher SD, RMSSD, and high frequency (HF) components of HRV than those of the control group. \u0000Conclusion: The primary result suggests that PC5 and PC6 electro-acupuncture affects cardiac autonomic neural regulation, mainly via the parasympathetic system, in subjects exposed to fear-invoking auditory stimulation.","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"16 1","pages":"98-109"},"PeriodicalIF":0.0,"publicationDate":"2017-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82896643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-29DOI: 10.21010/AJTCAM.VI15.1.2
P. Kempegowda, F. Zameer, Chethan Kumar Narasimashetty, S. Kollur, S. K. Murari
Background: Pharmacologic treatments for type 2 diabetes are based upon increasing insulin availability and improving sensitivity to insulin. Nowadays, glucagon like peptide-1 (GLP-1) based therapies aims at glucose control through DPP-4 inhibitors. DPP-4 is a transmembrane glycoprotein belongs to prolyl oligopeptidase family, with the specificity of removing X-Pro or X-Ala dipeptides from the N-terminus of polypeptides. GLP-1 effect by stimulating glucose-dependent insulin release from the pancreatic islets, inhibit inappropriate post-meal glucagon release and slow gastric emptying promoting leaky gut. The current study investigated DPP-4 inhibitory activity of catechin, isolated from Withania somnifera (WS), for ethnopharmacological treatment of type 2 diabetes and aimed to increase availability of GLP-1and sensitivity to insulin. Materials and Methods: Young and matured fresh roots, leaves, and fruits of WS plant extract were considered and were systematically evaluated for DPP-4 inhibitory activity using in vitro method, enzyme kinetics, phytochemical analysis, RP-HPLC, LCMS and 1H and 13C NMR method and structure-activity relationship (SAR) studies. Results: In this study, methanol (100% and 80%) extracts of WS matured root exhibited maximum DPP-4 inhibitory activity when compared to other extracts. The maximum DPP-4 inhibitory activity was found in 100% methanol extract of matured root. Phytobioactive was purified by RP-HPLC. The compound purified was found to be flavonoid and was characterized (LCMS, 1H and 13C NMR studies), identified as catechin. Auxiliary, molecular docking was performed using Ligand Fit method using PatchDock package. The study revealed the binding affinity of catechin with DPP-4 to be -6.601 kcal/mol with 13 hydrogen interactions with the receptor and was very similar to the standard potent blockers withaferin A and others (cuscohygrine, scopoletin, sitoindoside IV, tropine), further confirming its hyperglycemic potency. Conclusion: The study reveals that, 100% methanol extract of WS matured roots contains the compound- catechin, which exhibits DPP-4 inhibitory activity resulting in increased level of bioactive GLP-1 and GIP. In this background, we concluded that the WS will be a better source for further development as new antidiabetic drugs.
{"title":"INHIBITORY POTENCY OF WITHANIA SOMNIFERA EXTRACTS AGAINST DPP-4: AN IN VITRO EVALUATION","authors":"P. Kempegowda, F. Zameer, Chethan Kumar Narasimashetty, S. Kollur, S. K. Murari","doi":"10.21010/AJTCAM.VI15.1.2","DOIUrl":"https://doi.org/10.21010/AJTCAM.VI15.1.2","url":null,"abstract":"Background: Pharmacologic treatments for type 2 diabetes are based upon increasing insulin availability and improving sensitivity to insulin. Nowadays, glucagon like peptide-1 (GLP-1) based therapies aims at glucose control through DPP-4 inhibitors. DPP-4 is a transmembrane glycoprotein belongs to prolyl oligopeptidase family, with the specificity of removing X-Pro or X-Ala dipeptides from the N-terminus of polypeptides. GLP-1 effect by stimulating glucose-dependent insulin release from the pancreatic islets, inhibit inappropriate post-meal glucagon release and slow gastric emptying promoting leaky gut. The current study investigated DPP-4 inhibitory activity of catechin, isolated from Withania somnifera (WS), for ethnopharmacological treatment of type 2 diabetes and aimed to increase availability of GLP-1and sensitivity to insulin. \u0000Materials and Methods: Young and matured fresh roots, leaves, and fruits of WS plant extract were considered and were systematically evaluated for DPP-4 inhibitory activity using in vitro method, enzyme kinetics, phytochemical analysis, RP-HPLC, LCMS and 1H and 13C NMR method and structure-activity relationship (SAR) studies. \u0000Results: In this study, methanol (100% and 80%) extracts of WS matured root exhibited maximum DPP-4 inhibitory activity when compared to other extracts. The maximum DPP-4 inhibitory activity was found in 100% methanol extract of matured root. Phytobioactive was purified by RP-HPLC. The compound purified was found to be flavonoid and was characterized (LCMS, 1H and 13C NMR studies), identified as catechin. Auxiliary, molecular docking was performed using Ligand Fit method using PatchDock package. The study revealed the binding affinity of catechin with DPP-4 to be -6.601 kcal/mol with 13 hydrogen interactions with the receptor and was very similar to the standard potent blockers withaferin A and others (cuscohygrine, scopoletin, sitoindoside IV, tropine), further confirming its hyperglycemic potency. \u0000Conclusion: The study reveals that, 100% methanol extract of WS matured roots contains the compound- catechin, which exhibits DPP-4 inhibitory activity resulting in increased level of bioactive GLP-1 and GIP. In this background, we concluded that the WS will be a better source for further development as new antidiabetic drugs.","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"105 1","pages":"11-25"},"PeriodicalIF":0.0,"publicationDate":"2017-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82225566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-29DOI: 10.21010/AJTCAM.VI15.1.11
Karla Marina Báez-Parra, L. Alcaraz-Melendez, Apolinar Santamaría-Miranda, J. B. Heredia, J. León‐Félix, M. Muy-Rangel, M. Angulo-Escalante
Background: Mexican damiana (Turnera diffusa Willd. Ex. Schult), specifically damiana of California has a high demand in the international market for its popularity as aphrodisiac, but its marketing has been affected by the adulteration of its products due to a lack of authentication mechanisms and limited information on the differences between the two currently known varieties. The aim of this study was to establish a leaf standard monograph with scientific bases of the varieties aphrodisiaca and diffusa of Turnera diffusa Willd. Ex. Schult, cultivated at the same agronomic and environmental conditions. Material and Methods: Damiana leaves were collected from a cultivar located in the municipality of Culiacan, Sinaloa, Mexico in 2016. The pharmacognostic studies were carried out in terms of macroscopic and both optical and electronic microscopic characteristics of leaves of the two varieties for their distinction. Results: The leaves of diffusa variety were twice as large as those of the aphrodisiaca variety, whereas papillose glandular trichomes were observed with greater density in the aphrodisiaca variety than those on diffusa leaves where unicellular trichomes were the ones observed in greater density. The leaves of both varieties are dorsiventral and hypostomatic with paracytic stomata. Conclusion: The obtained qualitative and quantitative leaf standards provide reference information for the proper identification and monograph preparation of the aphrodisiaca and diffusa varieties of Turnera diffusa. Pharmacognostic characteristics such as the type of stomata, trichomes, and leaf identify the species, whereas characteristics as leaf size and trichome density differ between varieties.
{"title":"LEAF MORPHOLOGY AND ANATOMY OF VARIETIES OF TURNERA DIFFUSA VAR. DIFFUSA AND TURNERA DIFFUSA VAR. APHRODISIACA (WARD) URB","authors":"Karla Marina Báez-Parra, L. Alcaraz-Melendez, Apolinar Santamaría-Miranda, J. B. Heredia, J. León‐Félix, M. Muy-Rangel, M. Angulo-Escalante","doi":"10.21010/AJTCAM.VI15.1.11","DOIUrl":"https://doi.org/10.21010/AJTCAM.VI15.1.11","url":null,"abstract":"Background: Mexican damiana (Turnera diffusa Willd. Ex. Schult), specifically damiana of California has a high demand in the international market for its popularity as aphrodisiac, but its marketing has been affected by the adulteration of its products due to a lack of authentication mechanisms and limited information on the differences between the two currently known varieties. The aim of this study was to establish a leaf standard monograph with scientific bases of the varieties aphrodisiaca and diffusa of Turnera diffusa Willd. Ex. Schult, cultivated at the same agronomic and environmental conditions. \u0000Material and Methods: Damiana leaves were collected from a cultivar located in the municipality of Culiacan, Sinaloa, Mexico in 2016. The pharmacognostic studies were carried out in terms of macroscopic and both optical and electronic microscopic characteristics of leaves of the two varieties for their distinction. \u0000Results: The leaves of diffusa variety were twice as large as those of the aphrodisiaca variety, whereas papillose glandular trichomes were observed with greater density in the aphrodisiaca variety than those on diffusa leaves where unicellular trichomes were the ones observed in greater density. The leaves of both varieties are dorsiventral and hypostomatic with paracytic stomata. \u0000Conclusion: The obtained qualitative and quantitative leaf standards provide reference information for the proper identification and monograph preparation of the aphrodisiaca and diffusa varieties of Turnera diffusa. Pharmacognostic characteristics such as the type of stomata, trichomes, and leaf identify the species, whereas characteristics as leaf size and trichome density differ between varieties.","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"120 1","pages":"110-116"},"PeriodicalIF":0.0,"publicationDate":"2017-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88755373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-29DOI: 10.21010/ajtcam.vi15.1.19
J. Nam, H. Jung, W. K. Kim, H. Bae
Background: The cast-off shells of Cryptotympana pustulata (Periostracum Cicadae, PC) and the bark of Betula platyphylla (Betulae Cortex, BC) are used as traditional medicines for the treatment of skin diseases. This study was conducted to investigate the regulatory effects of PC and BC extracts on the activation of the ion channels, calcium release-activated calcium channel protein 1 (ORAI1) and transient receptor potential cation channel subfamily V member 3 (TRPV3). Materials and Methods: Human HEK293T cells, co-overexpressing ORAI1/stromal interaction molecule 1 (STIM1) or overexpressing TRPV3, were treated with PC or BC extracts at 0.1 mg/mL. The changes in ORAI1 and TRPV3 activities were measured using a conventional whole-cell patch-clamp technique. Results: PC and BC extracts significantly decreased ORAI1 activation in ORAI1-STIM1 co-overexpressing HEK293T cells and significantly increased TRPV3 activation in TRPV3 overexpressing cells, compared to that of 2- aminoethoxydiphenyl borate (2-APB, 100 μM), a known agonist of TRPV3. Conclusion: Our results suggest that PC and BC extracts have therapeutic potential to improve skin barrier abnormalities in atopic dermatitis via modulation of ORAI1 and TRPV3 activation.
{"title":"MODULATORY EFFECT OF PERIOSTRACUM CICADAE AND BETULAE CORTEX EXTRACTS ON THE ACTIVATION OF ATOPIC DERMATITIS-RELATED ION CHANNELS ORAI1 AND TRPV3","authors":"J. Nam, H. Jung, W. K. Kim, H. Bae","doi":"10.21010/ajtcam.vi15.1.19","DOIUrl":"https://doi.org/10.21010/ajtcam.vi15.1.19","url":null,"abstract":"Background: The cast-off shells of Cryptotympana pustulata (Periostracum Cicadae, PC) and the bark of Betula \u0000platyphylla (Betulae Cortex, BC) are used as traditional medicines for the treatment of skin diseases. This study was \u0000conducted to investigate the regulatory effects of PC and BC extracts on the activation of the ion channels, calcium \u0000release-activated calcium channel protein 1 (ORAI1) and transient receptor potential cation channel subfamily V \u0000member 3 (TRPV3). \u0000Materials and Methods: Human HEK293T cells, co-overexpressing ORAI1/stromal interaction molecule 1 (STIM1) \u0000or overexpressing TRPV3, were treated with PC or BC extracts at 0.1 mg/mL. The changes in ORAI1 and TRPV3 \u0000activities were measured using a conventional whole-cell patch-clamp technique. \u0000Results: PC and BC extracts significantly decreased ORAI1 activation in ORAI1-STIM1 co-overexpressing HEK293T \u0000cells and significantly increased TRPV3 activation in TRPV3 overexpressing cells, compared to that of 2- \u0000aminoethoxydiphenyl borate (2-APB, 100 μM), a known agonist of TRPV3. \u0000Conclusion: Our results suggest that PC and BC extracts have therapeutic potential to improve skin barrier \u0000abnormalities in atopic dermatitis via modulation of ORAI1 and TRPV3 activation.","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"19 1","pages":"183-187"},"PeriodicalIF":0.0,"publicationDate":"2017-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75587430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-29DOI: 10.21010/AJTCAM.VI15.1.16
Ming Wu, Q. Zhang, Jingyuan Song, Xiwen Li, C. Xie, Zhigang Hu
Background: As a rare and endangered medicinal plant growing in plateaus, Fritillaria cirrhosa D. Don is a scant resource in terms of quantity and planting regions. However, there is limited knowledge on predicting the potential ecological suitability of regions for the species with the climate factors. This paper evaluates the ecological suitability of F. cirrhosa D. Don on a global scale using Maxent. Materials and Methods: The ecologically suitable regions for F. cirrhosa D. Don around the world were evaluated using the maximum entropy model (Maxent), based on 127 known occurrence records and specific environmental predictor variables. Results: The Maxent model was highly accurate, with a statistically significant Area Under the Receiving Operator Curve (AUC) value of 0.993, and the most suitable areas and the suitable areas for F. cirrhosa D. Don were approximately 450,000 and 700,000 sq. km., respectively, including China, Pakistan, Nepal, and Bhutan. A quantitative study of the climatic characteristics of F. cirrhosa D. Don indicated that the period from May to October was critical for plant growth and development. Thus, the stable precipitation-temperature ratios (0.59 to 2.42) during this period could serve as a feature indicator for the geographical distribution of the plant. Conclusion: This work should be beneficial for the introduction and resource protection of F. cirrhosa D. Don, meanwhile, the analytical method could be expanded to predict the potential distribution of other medicinal plants.
背景:川贝母是一种生长在高原的珍稀濒危药用植物，在数量和种植区域上资源匮乏。然而，利用气候因子预测该物种的潜在生态适宜性的知识有限。本文利用Maxent在全球范围内评估了肝硬化F. D. Don的生态适宜性。材料和方法:基于127个已知的发生记录和特定的环境预测变量，使用最大熵模型(Maxent)对全球范围内肝硬化d.d Don的生态适宜区域进行评估。结果:Maxent模型准确率较高，接收算子曲线下面积(Area Under The Receiving Operator Curve, AUC)值为0.993，具有统计学意义，肝硬化F. D. Don的最适宜面积和适宜面积分别约为45万平方和70万平方。公里。包括中国、巴基斯坦、尼泊尔和不丹。一项定量研究表明，5月至10月是植物生长发育的关键时期。因此，稳定的降水-温度比值(0.59 ~ 2.42)可作为该植物地理分布的特征指标。结论:本研究为肝硬化金盏花的引种和资源保护提供了有益的依据，同时也可将该分析方法扩展到其他药用植物的潜在分布预测。
{"title":"ECOLOGICAL CHARACTERISTICS AND SUITABILITY EVALUATION OF FRITILLARIA CIRRHOSA D. DON BASED ON MAXENT MODEL","authors":"Ming Wu, Q. Zhang, Jingyuan Song, Xiwen Li, C. Xie, Zhigang Hu","doi":"10.21010/AJTCAM.VI15.1.16","DOIUrl":"https://doi.org/10.21010/AJTCAM.VI15.1.16","url":null,"abstract":"Background: As a rare and endangered medicinal plant growing in plateaus, Fritillaria cirrhosa D. Don is a scant resource in terms of quantity and planting regions. However, there is limited knowledge on predicting the potential ecological suitability of regions for the species with the climate factors. This paper evaluates the ecological suitability of F. cirrhosa D. Don on a global scale using Maxent. \u0000Materials and Methods: The ecologically suitable regions for F. cirrhosa D. Don around the world were evaluated using the maximum entropy model (Maxent), based on 127 known occurrence records and specific environmental predictor variables. \u0000Results: The Maxent model was highly accurate, with a statistically significant Area Under the Receiving Operator Curve (AUC) value of 0.993, and the most suitable areas and the suitable areas for F. cirrhosa D. Don were approximately 450,000 and 700,000 sq. km., respectively, including China, Pakistan, Nepal, and Bhutan. A quantitative study of the climatic characteristics of F. cirrhosa D. Don indicated that the period from May to October was critical for plant growth and development. Thus, the stable precipitation-temperature ratios (0.59 to 2.42) during this period could serve as a feature indicator for the geographical distribution of the plant. \u0000Conclusion: This work should be beneficial for the introduction and resource protection of F. cirrhosa D. Don, meanwhile, the analytical method could be expanded to predict the potential distribution of other medicinal plants.","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"9 1","pages":"158-167"},"PeriodicalIF":0.0,"publicationDate":"2017-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89941692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-29DOI: 10.21010/AJTCAM.VI15.1.12
Solomon Oladele Oladeji, E. A. Agbelusi
Background: Understanding the intricate link between humans and the plants use is needed to address some of to-day’s socio-economic and health challenges. Materials and Methods: The study focused on plants that have high cultural and medicinal values for the host communities of Old Oyo National Park, Nigeria. Local Knowledge was captured through focus group discussions with herbal vendors, interviews with herbalists as key informants and through field observations. Snowball sampling technique was used in selecting the key informants. Participants selected for the Focus Group Discussion varies with the number of herbal vendors encountered in each commercial market. Results: In total, 78 medicinal plant species belonging to 39 families including 47 trees, 8 shrubs and 23 herbaceous plants were documented. Forty ailments categorized into thirty one treatments and prevention methods were discovered. Kigellia africana, Cassia spp and Alstonia boonei were used for treating common ailments like malaria, dysentery and gonorrhea. Sources of collecting the medicinal plant species and their mode of administration were different. Conclusion: The research outcome will contribute to advancement of traditional medicine, resource efficiency and development of new enterprise among the locals. Comprehensive quantitative study of social- economic importance of the medicinal plants is recommended.
{"title":"Capturing indigenous knowledge on medicinal plants use: Case study of selected communities in old Oyo National Park, Nigeria","authors":"Solomon Oladele Oladeji, E. A. Agbelusi","doi":"10.21010/AJTCAM.VI15.1.12","DOIUrl":"https://doi.org/10.21010/AJTCAM.VI15.1.12","url":null,"abstract":"Background: Understanding the intricate link between humans and the plants use is needed to address some of to-day’s socio-economic and health challenges. \u0000Materials and Methods: The study focused on plants that have high cultural and medicinal values for the host communities of Old Oyo National Park, Nigeria. Local Knowledge was captured through focus group discussions with herbal vendors, interviews with herbalists as key informants and through field observations. Snowball sampling technique was used in selecting the key informants. Participants selected for the Focus Group Discussion varies with the number of herbal vendors encountered in each commercial market. \u0000Results: In total, 78 medicinal plant species belonging to 39 families including 47 trees, 8 shrubs and 23 herbaceous plants were documented. Forty ailments categorized into thirty one treatments and prevention methods were discovered. Kigellia africana, Cassia spp and Alstonia boonei were used for treating common ailments like malaria, dysentery and gonorrhea. Sources of collecting the medicinal plant species and their mode of administration were different. \u0000Conclusion: The research outcome will contribute to advancement of traditional medicine, resource efficiency and development of new enterprise among the locals. Comprehensive quantitative study of social- economic importance of the medicinal plants is recommended.","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"14 1","pages":"117-136"},"PeriodicalIF":0.0,"publicationDate":"2017-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77023614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-29DOI: 10.21010/AJTCAM.VI15.1.1
Yunxia Guo, Junxiu Zhang, Shaodan Li, Yin Zhang, Yi Liu, Ming-Hui Yang
Background: Parkinson’s disease is a neurodegenerative disease, while its mechanism is still unclear. Long-term levodopa-based treatment leads to decreased response or loss of response, as well as severe side effects. Our previous study has proved that Bushenhuoxue Granules have effects on Parkinson’s disease, but the underlying mechanism is still need to be explored. Our research is to investigate the mechanisms of Bushenhuoxue Granules on Parkinson’s disease (PD) by examining changes in the expression of the adenosine A 2A receptor、vesicular monoamine transporter 2 (VMAT2)、divalent metal transporter 1（DMT1） and nuclear factor E2 related (Nrf2) in a rat model of Parkinson’s disease (PD) . Materials and Methods : Changes in the apomorphine (APO)-induced rotational behavior of rats were observed after treatment. Immunofluorescence and immunohistochemistry were performed to investigate changes in adenosine A2A receptor 、VMAT2、DMT1 and Nrf2 expression in the rat striatum and substantia nigra. Results: Rotations after treatment were199.11 ± 27.16, which significantly decreased compared with that before treatment ( 273.0 ± 44.61, p < 0.01). Adenosine A 2A receptor expression in the striatum was 3.10 ± 0.34 significantly increased in the model group and decreased in the normal control group, whereas the expression level in the Bushenhuoxue group was 1.13 ± 0.23,p < 0.05 between the two control groups. No adenosine A 2A receptor expression was observed in the substantia nigra. VMAT2 expression in the rat striatum was 23.20 ± 2.68 and substantia nigra was 15.98 ± 0.70 increased in the normal control group. They were 8.99 ± 0.48 in the rat striatum and 8.45 ± 0.59 substantia nigra significantly decreased in the model control group, whereas the expression level in the Bushenhuoxue group was 15.36 ± 0.89 in the rat striatum and 11.69 ± 1.17 in the rat substantia nigra (p < 0.05), also between the two control groups. DMT1 expression in the rat striatum was 3.30 ± 0.30 and substantia nigra was 6.56 ± 0.64 decreased in the normal control group. They were 7.92 ± 0.52 in the rat striatum and 12.76 ± 0.86 substantia nigra significantly increased in the model control group, whereas the expression level in the Bushenhuoxue group was 6.17 ± 0.27 in the rat striatum and 9.13 ± 0.44 in the rat substantia nigra (p < 0.05), also between the two control groups. Nrf2 expression in the rat striatum was 7.90 ± 0.29 and substantia nigra was 15.22 ± 1.22 increased in the normal control group. They were 3.09 ± 0.43 in the rat striatum and 8.57 ± 0.54 substantia nigra significantly decreased in the model control group, whereas the expression level in the Bushenhuoxue group was 5.00 ± 0.34 in the rat striatum and 12.46 ± 0.62 in the rat substantia nigra (p< 0.05), also between the two control groups. Conclusion : Bushenhuoxue Granules significantly improved the rotational behavior of PD’s rats, decreased adenosine A 2A receptor expression, and increased VMAT2 expression; decreas
{"title":"METABOLIC CHANGES INDUCED BY BUSHENHUOXUE GRANULES ON STRIATUM AND SUBSTANTIA NIGRA IN A RAT MODEL OF PARKINSON’S DISEASE","authors":"Yunxia Guo, Junxiu Zhang, Shaodan Li, Yin Zhang, Yi Liu, Ming-Hui Yang","doi":"10.21010/AJTCAM.VI15.1.1","DOIUrl":"https://doi.org/10.21010/AJTCAM.VI15.1.1","url":null,"abstract":"Background: Parkinson’s disease is a neurodegenerative disease, while its mechanism is still unclear. Long-term levodopa-based treatment leads to decreased response or loss of response, as well as severe side effects. Our previous study has proved that Bushenhuoxue Granules have effects on Parkinson’s disease, but the underlying mechanism is still need to be explored. Our research is to investigate the mechanisms of Bushenhuoxue Granules on Parkinson’s disease (PD) by examining changes in the expression of the adenosine A 2A receptor、vesicular monoamine transporter 2 (VMAT2)、divalent metal transporter 1（DMT1） and nuclear factor E2 related (Nrf2) in a rat model of Parkinson’s disease (PD) . Materials and Methods : Changes in the apomorphine (APO)-induced rotational behavior of rats were observed after treatment. Immunofluorescence and immunohistochemistry were performed to investigate changes in adenosine A2A receptor 、VMAT2、DMT1 and Nrf2 expression in the rat striatum and substantia nigra. Results: Rotations after treatment were199.11 ± 27.16, which significantly decreased compared with that before treatment ( 273.0 ± 44.61, p < 0.01). Adenosine A 2A receptor expression in the striatum was 3.10 ± 0.34 significantly increased in the model group and decreased in the normal control group, whereas the expression level in the Bushenhuoxue group was 1.13 ± 0.23,p < 0.05 between the two control groups. No adenosine A 2A receptor expression was observed in the substantia nigra. VMAT2 expression in the rat striatum was 23.20 ± 2.68 and substantia nigra was 15.98 ± 0.70 increased in the normal control group. They were 8.99 ± 0.48 in the rat striatum and 8.45 ± 0.59 substantia nigra significantly decreased in the model control group, whereas the expression level in the Bushenhuoxue group was 15.36 ± 0.89 in the rat striatum and 11.69 ± 1.17 in the rat substantia nigra (p < 0.05), also between the two control groups. DMT1 expression in the rat striatum was 3.30 ± 0.30 and substantia nigra was 6.56 ± 0.64 decreased in the normal control group. They were 7.92 ± 0.52 in the rat striatum and 12.76 ± 0.86 substantia nigra significantly increased in the model control group, whereas the expression level in the Bushenhuoxue group was 6.17 ± 0.27 in the rat striatum and 9.13 ± 0.44 in the rat substantia nigra (p < 0.05), also between the two control groups. Nrf2 expression in the rat striatum was 7.90 ± 0.29 and substantia nigra was 15.22 ± 1.22 increased in the normal control group. They were 3.09 ± 0.43 in the rat striatum and 8.57 ± 0.54 substantia nigra significantly decreased in the model control group, whereas the expression level in the Bushenhuoxue group was 5.00 ± 0.34 in the rat striatum and 12.46 ± 0.62 in the rat substantia nigra (p< 0.05), also between the two control groups. Conclusion : Bushenhuoxue Granules significantly improved the rotational behavior of PD’s rats, decreased adenosine A 2A receptor expression, and increased VMAT2 expression; decreas","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"57 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2017-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74941035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-29DOI: 10.21010/AJTCAM.VI15.1.5
Gérard A. Toudji, Emmanuel A. M. Thiombiano, S. Karou, K. Anani, Y. Adjrah, H. E. Gbekley, M. Kiendrebeogo, Y. Ameyapoh, J. Simporé
Background: Pithecellobium dulce (Roxb.) Benth., Securidaca longepedunculata Fresn and Cryptolepis sanguinolenta (Lindl.) Schlt are three plants widely used in the Togolese traditional medicine to treat microbial infections. Some studies reported their antibacterial activity alone but until know there no data concerning their possible interaction with conventional antibiotics. The main objective of this study was to investigate the antibacterial activity of the association of the crude extracts of the three plants with some conventional antibiotics. We further evaluate the antioxidant and the anti-inflammatory activities of the extracts on rat’s model. Materials and methods:The antimicrobial activity was evaluated by the broth microdilution assay and the Fractional inhibitory concentrations (FIC) determined by the checkerboard method. The anti-inflammatory activity was evaluated using the Carrageenan- induced rat paw edema model. The antioxidant activities and the phenol contents were determined by spectrophotometry. Results: The MICs of hydroethanolic extract of plants ranged from 3.125 to 100 mg/mL on Klebsiella pneumoniae strains. Synergistic action was observed only with the combination of Imipenem/P. dulce, imipenem/C. sanguinolenta, amikacin/P. dulce and amikacin/C. sanguinolenta against the ESBL negative Klebsiella pneumoniae strain. Of the 21 associations, 15 were antagonistic on the ESBL-producing strains. The indifference effect was observed with the combination of the extract of Securidaca longepedunculata and the following antibiotics imipenem, amikacin, tetracyclin, ciprofloxacin, Cefotaxim; and Sulfametoxazol+Trimethoprim. The in vitro anti-inflammatory with Lipoxygenase inhibition activity was best with C. sanguinolenta extract while the in vivo paw edema model revealed that S. longepedunculata was the highest reducer of paw edema. In addition white blood cells count and biochemical parameters such as total proteins and immunoglobulins were significantly affected by the administration of plant extracts. Conclusion: This study revealed that the three plants although they may inhibit the bacterial growth by themselves, but there is also a possible synergistic action with the commercial antibiotics. Further investigations are needed to identify the active compounds and their mechanism of action.
{"title":"Antibacterial and anti-inflammatory activities of crude extracts of three Togolese medicinal plants against ESBL Klebsiella pneumoniae strains","authors":"Gérard A. Toudji, Emmanuel A. M. Thiombiano, S. Karou, K. Anani, Y. Adjrah, H. E. Gbekley, M. Kiendrebeogo, Y. Ameyapoh, J. Simporé","doi":"10.21010/AJTCAM.VI15.1.5","DOIUrl":"https://doi.org/10.21010/AJTCAM.VI15.1.5","url":null,"abstract":"Background: Pithecellobium dulce (Roxb.) Benth., Securidaca longepedunculata Fresn and Cryptolepis sanguinolenta (Lindl.) Schlt are three plants widely used in the Togolese traditional medicine to treat microbial infections. Some studies reported their antibacterial activity alone but until know there no data concerning their possible interaction with conventional antibiotics. The main objective of this study was to investigate the antibacterial activity of the association of the crude extracts of the three plants with some conventional antibiotics. We further evaluate the antioxidant and the anti-inflammatory activities of the extracts on rat’s model. \u0000Materials and methods:The antimicrobial activity was evaluated by the broth microdilution assay and the Fractional inhibitory concentrations (FIC) determined by the checkerboard method. The anti-inflammatory activity was evaluated using the Carrageenan- induced rat paw edema model. The antioxidant activities and the phenol contents were determined by spectrophotometry. \u0000Results: The MICs of hydroethanolic extract of plants ranged from 3.125 to 100 mg/mL on Klebsiella pneumoniae strains. Synergistic action was observed only with the combination of Imipenem/P. dulce, imipenem/C. sanguinolenta, amikacin/P. dulce and amikacin/C. sanguinolenta against the ESBL negative Klebsiella pneumoniae strain. Of the 21 associations, 15 were antagonistic on the ESBL-producing strains. The indifference effect was observed with the combination of the extract of Securidaca longepedunculata and the following antibiotics imipenem, amikacin, tetracyclin, ciprofloxacin, Cefotaxim; and Sulfametoxazol+Trimethoprim. The in vitro anti-inflammatory with Lipoxygenase inhibition activity was best with C. sanguinolenta extract while the in vivo paw edema model revealed that S. longepedunculata was the highest reducer of paw edema. In addition white blood cells count and biochemical parameters such as total proteins and immunoglobulins were significantly affected by the administration of plant extracts. \u0000Conclusion: This study revealed that the three plants although they may inhibit the bacterial growth by themselves, but there is also a possible synergistic action with the commercial antibiotics. Further investigations are needed to identify the active compounds and their mechanism of action.","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"139 1","pages":"42-58"},"PeriodicalIF":0.0,"publicationDate":"2017-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81762689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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