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In vitro Callus induction of Fenugreek (Trigonellafoenum-graecum L.) Using Different Media with Different Auxins Concentrations 胡芦巴离体愈伤组织诱导的研究使用不同培养基和不同生长素浓度
Pub Date : 2013-05-01 DOI: 10.5251/ABJNA.2013.4.3.243.251
Mawahib E. M. ElNour, Bader EldinA
A protocol of callus induction in fenugreek (Trigonellafoenum-graecumL.) from 8 20 days old in vitro grown cotyledons node explants cultured on Murashige and Skoog (MS) and B5((Gamborget al., 1958) media supplemented with different types and concentrations of growth regulators were tested in order to obtain the best callus formation. Two auxin types, αnaphthalene acetic acid (NAA), and 2,4-dichloro-phenoxyacetic acid (2,4-D) at four concentrations(0.5, 0.1, 1.5, and 2.0 mgL-1) with (0,0) as control in the two media were used in this study .The objective of this study was to determine the effect of different concentration of two types of auxins 2,4-D (2,4Dichlorophenoxy acetic acid) and NAA (Naphthalene acetic acid), on callus induction using cotyledons and hypocotyls explants of T.foenum –graecum. 100 percentage of primary callus induction rate and 2.5 callusing index were achieved on MS medium containing 2mg/l ,2,4-D hormone hypocotyls explants .The maximum value of callusing index (2.8) was obtained from MS medium containing 1.5 mg/l ,2,4D using hypocotyls and cotyledons explants. The maximum callus formation observed in the MS media containing 2.0mg/l NAA was 3.9±o.o8 in hypocotyls segment. The callus was compact in cotyledons and variable in hypocotyls segments and the colour is creamy.
在Murashige和Skoog (MS)和B5(Gamborget al., 1958)培养基上添加不同类型和浓度的生长调节剂,对8 ~ 20日龄胡巴(Trigonellafoenum-graecumL.)离体子叶节外植体进行愈伤组织诱导试验,以获得最佳愈伤组织形成。以α萘乙酸(NAA)和2,4-二氯-苯氧乙酸(2,4- d)为生长素,在0.5、0.1、1.5和2.0 mg / l的浓度下,以(0,0)为对照,研究了两种生长素2,4-d(2,4-二氯-苯氧乙酸)和NAA(萘乙酸)不同浓度对黄芪子叶和下胚轴外植体愈伤组织诱导的影响。在含2mg/l 2,4- d激素下胚轴外植体的MS培养基上,初生愈伤组织诱导率为100%,愈伤组织指数为2.5,在含1.5 mg/l 2,4- d激素下胚轴和子叶外植体的MS培养基上,愈伤组织指数最高,为2.8。在含有2.0mg/l NAA的MS培养基中,最大愈伤组织形成率为3.9±0。下胚轴节O8。愈伤组织在子叶上致密,在下胚轴裂片上变异,颜色呈乳白色。
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引用次数: 14
Sperm quality and testicular morphometry of rabbits fed dietary levels of water spinach (Ipomoea aquatica) leaf meal 饲粮水平菠菜叶粕对家兔精子质量和睾丸形态的影响
Pub Date : 2013-05-01 DOI: 10.5251/ABJNA.2013.4.3.352.357
T. Ahemen, A. Abu
A total of twenty four rabbits of mixed breeds aged 10 12 weeks and weighed between 900 – 1000g, were used in an experiment to determine the effect of feeding varying dietary levels of water spinach (Ipomoea aquatica) leaf meal on reproductive parameters and organ weights of male rabbits. The rabbits were randomly allocated into four dietary treatments, T1, T2 T3 and T4 containing 0%, 5%, 10% and 15% of water spinach leaf meal respectively. Feed and water were served ad libitum throughout the 8 weeks experimental period. At the end of the feeding trial, 3 rabbits per treatment were slaughtered and their reproductive organs were carefully dissected out and separated into different components. The testicular morphometry and cauda epididymal sperm characteristics were evaluated. The result showed no significant (P> 0.05) effect of diet on testicular morphometry and sperm characteristics. No significant (P>0.05) effect of diet was also observed on the internal organ weights of the male rabbits. This result shows that water spinach meal can be included up to 15% in rabbit diets without adverse effect on testicular morphometry and sperm quality of male rabbits.
本试验选用24只10 ~ 12周龄、体重900 ~ 1000g的杂交品种家兔,研究饲粮中添加不同水平的水菠菜叶粕对雄性家兔生殖参数和器官重量的影响。将家兔随机分为添加0%、5%、10%和15%水菠菜叶粉的T1、T2、T3和T4 4个饲粮处理。在8周的试验期内,饲料和水可随意供应。饲喂试验结束时,每组屠宰3只家兔,仔细解剖其生殖器官,并将其分成不同的部分。对睾丸形态和附睾尾部精子特征进行了评价。结果表明,饲粮对睾丸形态和精子特征无显著影响(P < 0.05)。饲粮对公兔内脏重量无显著影响(P < 0.05)。由此可见,在家兔饲粮中添加15%的水菠菜粉对雄性家兔睾丸形态和精子质量无不良影响。
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引用次数: 5
Sero-epidemiologic survey of the equine arteritis in the region of Khenchela (Algeria) 阿尔及利亚肯切拉地区马动脉炎血清流行病学调查
Pub Date : 2013-03-01 DOI: 10.5251/ABJNA.2013.4.2.132.136
E. Bererhi, R. Kabouia
The aim of this study is to evaluate the prevalence of respiratory infections of viral origin in equines .In this perspective we have researched ant viruses antibodies in equine’s arteritis in 95 horses of different breeds, Arab-Barb and Barb, all stationed in the region of Khenchela (Kais),located in the north east of Constantine. The animals are prealably recognized, upon a clinical exam, in a good health. They have received a diet containing barley and hay. They are of different sexes and their age is varying between 2 and 10 years old. The equines play an important socioeconomic role. In several rural areas, equines are regularly used as a tensile force for the animal traction cultivation and transport of persons and goods. They thus contribute widely to the increase of agricultural production and the improvement of socio economic conditions of rural populations. The results demonstrate that among the 95 serums tested, 25 were positive (33, 33 percent).to the reaction serum seroneutralization. These results are discussed in relation whith those observed by other authors. Key-words: Horse, arterits virus, serum neutralization, epidemiology.
本研究的目的是评估病毒源性呼吸道感染在马中的流行程度,从这个角度出发,我们研究了95匹不同品种的马(阿拉伯-Barb和Barb)的马动脉炎中的蚂蚁病毒抗体,这些马都驻扎在康斯坦丁东北部的Khenchela地区。根据临床检查,这些动物通常是健康的。他们得到了含有大麦和干草的食物。他们的性别不同,年龄在2岁到10岁之间。马扮演着重要的社会经济角色。在一些农村地区,马经常被用作动物牵引、耕作和运输人员和货物的拉力。因此,它们对增加农业生产和改善农村人口的社会经济条件作出了广泛的贡献。结果显示,在95份检测血清中,25份呈阳性(33.33%)。血清中和反应。这些结果与其他作者的观察结果相联系进行了讨论。关键词:马,动脉病毒,血清中和,流行病学。
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引用次数: 0
Impact of host's diet and parasite intensity on Morphology of Moniezia expansa 寄主食性和寄主强度对扩张念珠虫形态的影响
Pub Date : 2013-03-01 DOI: 10.5251/ABJNA.2013.4.2.122.125
J. A. Kuchai, F. Ahmad
The present study is a part of the helminthological study conducted on ruminants of Ladakh for the time period of one year. In the present study impact of diet of the host and intensity of the parasite on Morphology of a Cestode (Moniezia expansa) was taken into consideration for which postmortem examination of slaughtered host animals belonging to different age groups and of either sex feeding on different diets were carried out and the parasite/s recovered were processed and were subjected for the study of morphology and morphometry. It was observed that diet and intensity was having a relation with morphology and morphometry of the parasite. In case of higher intensity the size of parasite was comparatively small as compared to the specimens with low intensity, so was the case with respect to diet of the host it was observed that the parasites recovered from the host animals which feed on highly nutritive food were larger in size as compared to those specimens recovered from the hosts which feed on average or below average food. The parasite being reported for the first time from this region has been briefly redescribed with some intraspecific variations.
本研究是对拉达克反刍动物进行为期一年的蠕虫学研究的一部分。在本研究中,考虑了寄主的饮食和寄生虫的强度对一种腹腹绦虫(Moniezia expansa)形态的影响,并对屠宰的不同年龄组和不同性别的寄主动物进行了尸检,对回收的寄生虫进行了处理,并进行了形态学和形态计量学研究。结果表明,摄食和强度与寄生蜂的形态和形态测定有一定的关系。在高强度的情况下,与低强度的标本相比,寄生虫的大小相对较小,在宿主的饮食方面也是如此。我们观察到,从以高营养食物为食的宿主动物身上恢复的寄生虫比从以平均或低于平均食物为食的宿主动物身上恢复的寄生虫体积更大。本文对该地区首次报道的寄生虫进行了简要的重新描述,并发现了一些种内变异。
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引用次数: 2
Study on major causes of kid mortality in Adami Tulu Jido Kombolcha District of Oromia, Ethiopia 埃塞俄比亚奥罗米亚Adami Tulu Jido Kombolcha地区儿童死亡主要原因研究
Pub Date : 2013-03-01 DOI: 10.5251/ABJNA.2013.4.2.110.115
G. Debele, M. Duguma, Feyisa Hundessa, Firhowet Messele, M. Negash
A cross sectional investigation was conducted on the major causes of kids’ mortality in Adami Tulu Jiddo Kombolcha district from May 2009 to June 2010 to determine the mortality rate and to identify the potential causes of kids’ mortality in Arsi- Bale kids. A combination of multistage and purposive sampling techniques was used to select the study areas while simple random sampling was adopted to select experimental goats. The result revealed that from a total of 536 kids born over 12 months period about 22.4% (n=120) kids were died. Internal parasite assessment was conducted to see the infection level of parasites infection both on adult and kids of Arsi- Bale goats. Accordingly fecal sample was directly collected from the rectum of the experimental animal and its result showed that from a total of 39 adults and 158 kids fecal sample collected 59.6% ( n=118) was positive and 40.4% (n=80) was negative. The types of internal parasites mostly observed on goats of the study sites were identified as Strongly (90.5%), Monesia (23.3%), Coccidian (8.6%), Fasciola (1.7%) and Ascaris (0.9%). From the positive fecal samples, 51.5% (n=61) is highly infected, 19.5. %( n=23) moderately infected and 28.8 % (n= 34) had low infestation. Diarrhea, diseases and/or internal parasites were responsible for death of kids. Therefore, systematic control of internal parasites and diseases and periodic vaccination of goats against the identified diseases should be in place to improve productivity of goats for economic return.
2009年5月至2010年6月,对阿达米图鲁吉多孔博尔查区儿童死亡的主要原因进行了横断面调查,以确定Arsi- Bale儿童的死亡率并确定儿童死亡的潜在原因。研究区选择采用多阶段抽样和目的抽样相结合的方法,试验山羊选择采用简单随机抽样。结果显示,在12个月内出生的536名儿童中,约有22.4% (n=120)的儿童死亡。对阿西-贝尔山羊进行了体内寄生虫感染评估,以了解阿西-贝尔山羊成年山羊和幼山羊的寄生虫感染水平。因此,直接从实验动物的直肠采集粪便样本,结果表明,在39例成人和158例儿童粪便样本中,59.6% (n= 118)为阳性,40.4% (n=80)为阴性。研究点山羊体内寄生虫类型主要为强寄生虫(90.5%)、念珠虫(23.3%)、球虫(8.6%)、片形吸虫(1.7%)和蛔虫(0.9%)。在阳性粪便样本中,51.5% (n=61)为高度感染,19.5%。中度侵染占%(n=23),轻度侵染占28.8% (n= 34)。腹泻、疾病和/或体内寄生虫是造成儿童死亡的原因。因此,应系统地控制山羊体内寄生虫和疾病,并定期为山羊接种预防已确定疾病的疫苗,以提高山羊的生产力,获得经济回报。
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引用次数: 9
Effect of Teak (Tectona grandis) and Rubber (Elaeis guineensis) Plantations on Soil Physico-Chemical Properties of Alfisol and Ultisol in Parts of Nigeria 尼日利亚部分地区柚木和橡胶人工林对Alfisol和Ultisol土壤理化性质的影响
Pub Date : 2013-03-01 DOI: 10.5251/ABJNA.2013.4.2.137.144
A. Iwara, F. Ogundele
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引用次数: 0
Decommissioned dates: chemical composition and fermentation substrate for the production of extracellular catalase by an Aspergillus phoenicis mutant 退役日期:由凤凰曲霉突变体生产胞外过氧化氢酶的化学成分和发酵底物
Pub Date : 2013-01-01 DOI: 10.5251/ABJNA.2013.4.1.41.47
K. N., D. L, M. z, Destain J, Kahlat K, Thonart Ph
The recovery of dates downgraded as a fermentation medium for the production of extracellular catalase by Aspergillus phoenicis K30 was studied. Analysis of the chemical composition of pulp and kernel flour of dates showed that the pulp had a considerably greater carbohydrate content compared to the kernel (84 vs 2.93% respectively). However, the kernel flour was richer in nitrogen (0.68% vs 0.34), mineral elements (3.63 vs 1.28%) and in essential fatty acids C18: 2 vs C18: 3 than the pulp flour. The soluble extract of the date flour showed that sugars solubilised at 90% consisted of sucrose, fructose and glucose. Therefore, this extract, being an important source of carbon and energy, was used in the current study as a fermentation medium (after supplementation with 20% of corn steep) for the production of extracellular catalase by A. Phoenicis K30. During the course of this fermentation, the biomass was estimated at 18.6 g / L after 72 h of culture, while the maximum concentration of extracellular catalase (47.5 U / ml) was reached at 96 h of fermentation. The mycelium obtained in pellet form is suitable for industrial exploitation of this process.
研究了凤凰曲霉K30生产胞外过氧化氢酶的发酵培养基中枣渣的回收。对大枣果肉和果仁粉的化学成分分析表明,果肉的碳水化合物含量明显高于果仁(分别为84%和2.93%)。籽粒粉的氮含量(0.68% vs 0.34)、矿质元素(3.63 vs 1.28%)和必需脂肪酸(C18: 2 vs C18: 3)均高于果肉粉。枣粉的可溶性提取物表明,90%的可溶性糖由蔗糖、果糖和葡萄糖组成。因此,作为碳和能量的重要来源,本研究将该提取物作为a . Phoenicis K30生产胞外过氧化氢酶的发酵培养基(添加20%的玉米浸泡液后)。在发酵过程中,72h后的生物量估计为18.6 g / L,而胞外过氧化氢酶在96h时达到最大浓度(47.5 U / ml)。以球团形式得到的菌丝体适合于该工艺的工业化开发。
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引用次数: 8
Study of antagonistic yeasts isolated from some natural sources of West Bengal 西孟加拉邦一些天然来源拮抗酵母的研究
Pub Date : 2013-01-01 DOI: 10.5251/ABJNA.2013.4.1.33.40
Swapan Kr, Tumpa Santra, Amit Chakravarty
An intensive survey was conducted and 196 samples were collected from different zones of 12 districts of West Bengal. The collected samples were various fruits ( apple , green grapes . brown grapes , date fruits , jamun and fig ) ,juices ( date juice , toddy , cane juice ) and milk. From these samples 588 yeast colonies were isolated .These yeasts were identified by conventional morphological, microscopical and biochemical testing methods. These colonies comprise of 23 genera and 38 species of yeasts. Out of 38 isolated & identified species, 26 are ascomycetous while 12 are basidiomycetous yeasts .Maximum number of species were found in Candida (5) and followed by Pichia(4), Rhodotorula(3) and Saccharomyces (2).The percent of occurrence , Berger – Parker dominance index of each species were calculated. All yeast species isolated were screened by dual culture plate method for their antagonistic property against Penicillium digitatum, causal pathogen of Penicillium rot of Citrus . Out of 38 species of yeasts,15 species were antagonistic to Penicillium digitatum. Candida famata gave maximum percent of radial inhibition ( 70.24 PIRG)followed by Pichia membranifaciens (68.21PIRG) and Rhodotorula mucilaginosa (60.56 PIRG).Therefore , these natural sources of West Bengal are good habitats of various yeast species including antagonistic yeasts and the antagonistic yeasts can be applied as biological control agents against post harvest Penicillium rot disease of Citrus
进行了密集调查,从西孟加拉邦12个县的不同地区收集了196个样本。收集的样品是各种水果(苹果、青葡萄)。黑葡萄、枣、果酱和无花果、果汁(枣汁、棕榈酒、甘蔗汁)和牛奶。从这些样品中分离出588个酵母菌菌落,并对这些酵母菌进行了常规形态学、显微和生化鉴定。这些菌落包括23属和38种酵母。分离鉴定的38种酵母中,子囊菌26种,担子菌12种,种类最多的是念珠菌(5种),其次是毕赤酵母(4种)、红酵母(3种)和酵母菌(2种),并计算了每种酵母的Berger - Parker优势度指数。采用双培养平板法对分离得到的酵母菌对柑橘霉病病原菌指状青霉的拮抗性能进行了筛选。38种酵母菌中有15种对指状青霉具有拮抗作用。念珠菌的径向抑制率最高(70.24 PIRG),其次是膜性毕赤酵母(68.21PIRG)和粘液红酵母(60.56 PIRG)。因此,西孟加拉邦的这些天然资源是各种酵母菌的良好栖息地,包括拮抗酵母菌,拮抗酵母菌可以作为柑橘采后青霉腐病的生物防治剂
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引用次数: 10
Dried egg powder utilization, a new frontier in bakery products 干蛋粉的利用——烘焙产品的新前沿
Pub Date : 2012-12-01 DOI: 10.5251/ABJNA.2012.3.12.493.505
A. Asghar, M. Abbas
Eggs are very nutritious and healthy, both egg white and egg yolk have high biological value. Egg albumen, also called egg white contains a viscous 10 % solution which consists of minerals, peptides and proteins in water. These proteins have high nutritional value for humans due to their specific amino acids composition. In addition, the egg white has specific functional properties in food processing like foaming and stability properties. Major functional properties of whole egg are stability, emulsification, foaming and gelling ability. Whole egg is also applied as colorants. These nutritional properties of egg are applicable in bakery foods, mayonnaise products, salad dressings, confections, ice cream, pastas and many convenience foods. Powdered egg can be used in several preparations during processing of food in place of using liquid egg. Egg powder from spray drying has a better structure as compared to the powder from foam mat drying. Hence, powder from spray drying process can be utilized as an essential ingredient in preparations of cakes, custards, noodles and some of the confectionery products.
鸡蛋很有营养和健康,蛋清和蛋黄都有很高的生物价值。蛋清,也叫蛋清,含有10%的粘性溶液,由水中的矿物质、多肽和蛋白质组成。由于其特定的氨基酸组成,这些蛋白质对人体具有很高的营养价值。此外,蛋清在食品加工中具有特定的功能特性,如发泡性和稳定性。全蛋的主要功能特性是稳定性、乳化性、起泡性和胶凝性。整个鸡蛋也可用作着色剂。鸡蛋的这些营养特性适用于烘焙食品、蛋黄酱产品、沙拉酱、糖果、冰淇淋、意大利面和许多方便食品。在食品加工过程中,鸡蛋粉可以用来代替液体鸡蛋。喷雾干燥的蛋粉比泡沫垫干燥的蛋粉具有更好的结构。因此,喷雾干燥过程中的粉末可以用作蛋糕、蛋奶冻、面条和一些糖果产品的基本成分。
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引用次数: 14
Effect of extracts from leaves, bark and root of Azadiratcha indica L on the vegetative growth of Colletrichum gloeosporoides :field soft rot pathogen of soursop (Annona muricata L ) fruit 印楝叶、树皮和根提取物对番荔枝果实软腐病病原菌——gloeosporoides营养生长的影响
Pub Date : 2012-12-01 DOI: 10.5251/ABJNA.2012.3.12.481.485
C. Nweke, O. Ibiam
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引用次数: 3
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Agriculture and Biology Journal of North America
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