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Larvicidal properties of Eugenia uniflora leaves 单叶Eugenia uniflora叶片的杀幼虫特性
Pub Date : 2012-10-01 DOI: 10.5251/abjna.2012.3.10.400.405
F. Famuyiwa, A. C. Adebajo
Eugenia uniflora is used ethnomedicinally in Nigeria for diseases including malaria and in Brazil the leaves are spread on the floor for its insect-repellent activity. Search for plants useful in the control of vector of Dengue fever, necessitated investigation of the activity of E. uniflora leaf against fourth instars larvae of Aedes aegyptii, using bioactivity guided fractionation. Larvicidal activity (LC50 2.74 and 2.18 mg/ml at 24 and 48 h) of the leaf methanolic extract, comparable (p > 0.05) to Endosulphan (LC50 0.93 and 0.90 mg/ml at 24 and 48 h), was concentrated in the nhexane partition fraction (LC50 2.58 and 1.87 mg/ml at 24 and 48 h). Vacuum liquid chromatography of n-hexane fraction gave fifth and sixth bulked fractions with significantly (p 0.05) to that of Endosulphan and better than their mother fractions. The results established the larvicidal activity of E. uniflora leaf and encouraged further development of these fractions as alternative larvicide in the control of Dengue fever, especially among the rural populations of Africa.
uniflora Eugenia uniflora在尼日利亚的民族医学上用于治疗包括疟疾在内的疾病,在巴西,其叶子被铺在地板上,因为它具有驱虫活性。为了寻找控制登革热媒介的有效植物,有必要采用生物活性引导分馏法研究单叶伊蚊叶片对埃及伊蚊4龄幼虫的活性。叶甲醇提取物的杀虫活性(24和48 h时LC50分别为2.74和2.18 mg/ml)与内生磺胺(24和48 h时LC50分别为0.93和0.90 mg/ml)相当(p >.05),正己烷部分(24和48 h时LC50分别为2.58和1.87 mg/ml),正己烷部分的第5和第6部分与内生磺胺有显著性差异(p 0.05),且优于其母部分。结果表明,单叶叶具有一定的杀幼虫活性,并鼓励进一步开发单叶叶作为登革热防治的替代杀幼虫剂,特别是在非洲农村人群中。
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引用次数: 10
Development of immunological and molecular test systems for identification and typification of equine influenza virus 马流感病毒鉴定和分型的免疫学和分子检测系统的发展
Pub Date : 2012-10-01 DOI: 10.5251/ABJNA.2012.3.10.406.412
Matveyeva Mikhailovna, K. Kaukarbayevich
A competitive version of enzyme-linked immunosorbent assay (ELISA) based on usage of antibodies and conjugates to ribonucleoprotein (RNP) for screening of antibodies to certain subtypes of equine influenza virus has been developed as well as reverse transcription polymerase chain reaction (RT-PCR) for identification and typification of equine influenza virus, subtype H3, in biological materials.
基于抗体和核糖核蛋白偶联物(RNP)筛选马流感病毒某些亚型抗体的酶联免疫吸附测定(ELISA)的竞争性版本已经开发出来,以及用于鉴定和分型马流感病毒H3亚型生物材料的逆转录聚合酶链反应(RT-PCR)。
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引用次数: 0
Effect of storage conditions and duration on the potency of Neem (Azadirachta indica A. Juss) seeds as a home-made Insecticide 贮存条件和贮存时间对印楝种子自制杀虫剂效力的影响
Pub Date : 2012-10-01 DOI: 10.5251/ABJNA.2012.3.10.385.390
H. E. Shafie, A. Almahy
This work was carried out to investigate the effects of grinding, storage conditions and duration on the bio-efficacy of neem seeds as a home-made insecticide against the red flour beetle Tribolium castaneum. Samples of neem seeds (intact and ground) were kept in small jute sacks and stored in the sun, refrigerator (4oC), and room temperature (28 ± 2oC) for two, four and six weeks in each condition. After the respective storage period, a water extract (5%) was prepared and sprayed topically on the 3 rd instar larvae of the beetle as well as on the pupal stage. A significant mortality of 62.83% resulted from the neem seeds stored in the refrigerator on the 7 th day of exposure. The seeds stored in the sun caused a mortality of 48.49%. The water extract from the seeds which were stored at room temperature, resulted in significantly high cumulative larval mortality of 50.30%. The efficacy of neem seeds kept in the sun was negatively affected and correlated with the length of storage period. However, the efficacy of the neem seeds stored in the refrigerator was increased with storage time. The pupal mortality due to spraying with water extract from neem seeds stored in the sun, refrigerator and room temperature was 49.67, 68.12 and 69.43% respectively. The mortality in the control (water treatment) was 7.33%. The results indicated that grinding of neem seeds before storage seems to have no effects on their efficacy.
本文研究了国产印楝种子在粉碎、贮存条件和贮存时间等条件下对红粉甲虫生物药效的影响。将完整磨碎的印楝籽样品保存在小黄麻袋中,分别在阳光下、冰箱(4℃)和室温(28±20℃)下保存2周、4周和6周。在相应的贮藏期后,制备5%的水提取物,局部喷洒在3龄幼虫和蛹期。印楝种子暴露第7天的死亡率为62.83%。在日光下贮藏的种子死亡率为48.49%。种子水提液室温保存后,累积幼虫死亡率高达50.30%。印楝种子在日光下的保鲜效果与贮藏时间呈负相关。然而,印楝种子在冰箱中储存的功效随着储存时间的增加而增加。喷施印楝种子水提液对日晒、冰箱和室温贮藏的蛹死亡率分别为49.67%、68.12%和69.43%。对照组(水处理)死亡率为7.33%。结果表明,在储存前研磨印楝种子似乎对其功效没有影响。
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引用次数: 11
Contribution to the flora of Um Rimmitta area, White Nile State, Sudan 对苏丹白尼罗州Um Rimmitta地区植物群的贡献
Pub Date : 2012-10-01 DOI: 10.5251/ABJNA.2012.3.10.427.434
A. Elsafori
The study deals with the ecology and taxonomy of the vegetation of Um Rimmitta area, Central Sudan. Taxonomic studies included collection, preparation, identification and classification of plant species in the study area. A total of 85 plant species were identified belonging o 30 families, represent the flora of the study area. From the identified species of the present study, (42) species were found to have medicinal values, and these represented (49.4%), of the total plant species of the study area. Moreover, a check-list of endangered, extinct and famine food plants has been provided. The study shown that during the drought spells in 1975, 1980, some of the plant species at the study area were lost. Also the study found that some plant species supported that locals during the period s of drought and bridged the gap in food supplies. The present study revealed that the soil moisture content was relatively low at the study area. The chemical analysis of the soil shown that there were no significant differences between sites in the study area with respect to soil elements. However, there were significant different between the sites in their pH values.
研究了苏丹中部Um Rimmitta地区植被的生态学和分类学。分类学研究包括研究区植物种类的收集、制备、鉴定和分类。共鉴定出植物85种,隶属于30科,代表了研究区区系。在本研究鉴定的植物种类中,有药用价值的有42种,占研究区植物种类总数的49.4%。此外,还提供了一份濒危、灭绝和饥荒粮食植物的核对清单。研究表明,在1975年和1980年的干旱期间,研究区域的一些植物物种消失了。研究还发现,一些植物物种在干旱期间支持当地人,弥补了食物供应的缺口。研究表明,研究区土壤含水量相对较低。土壤化学分析表明,研究区各立地土壤元素含量无显著差异。但不同位点的pH值存在显著差异。
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引用次数: 4
llness pattern and the relationship between the prevalence of malaria and other infections in Niger Delta University 疾病模式和疟疾流行率与尼日尔三角洲大学其他感染之间的关系
Pub Date : 2012-10-01 DOI: 10.5251/ABJNA.2012.3.10.413.426
C. Madukosiri, D. Bawo
The illness pattern and the relationship between malaria and other infections were examined in the present work – a base-line information prior to a meaningful healthcare policy formulation. A period of 5years (2007 – 2011) illness pattern was retrospectively obtained from the University Medical Centre, while questionnaires addressing the various health-related activities were administered to 500 volunteer subjects - students and staff of the institution. Biochemical parameters in the various disease conditions were determined using standard tests, while the means of frequencies of disease occurrence were compared using statistical package for social sciences (SPSS). Results of mean comparison showed that the mean of frequencies of malaria infection was significantly increasing (p 0.05). Malaria infection was the most prevalent illness with the highest frequency occurring around the month of April, accounting for 63.83 – 67.10% of the illnesses compared. This was followed by upper respiratory tract infection (URTI), peptic ulcer, and candidiasis, in that order. The relationship between the illness types showed that a positive correlation existed between malaria and typhoid, and malaria and URTI, (p<0.05). From the results of questionnaire analysis, 70.89% of the male students’ group admitted having a frequency of one to twelve attacks of malaria infection per annum with 43.67% of them who claimed to have suffered previous typhoid infections. As a result, 25.32% were absent from lectures for a period of 1- 30 days per annum. These results showed that malaria infection was on the increase and apart from the likelihood of impacting negatively on students’ performance, could represent a huge source of financial drain on a developing economy.
在本工作中审查了疾病模式和疟疾与其他感染之间的关系,这是制定有意义的保健政策之前的基线信息。从大学医疗中心回顾性地获得了5年(2007 - 2011年)的疾病模式,同时对500名志愿者(该机构的学生和工作人员)进行了关于各种健康相关活动的问卷调查。不同疾病条件下的生化参数采用标准检验确定,疾病发生频率均值采用SPSS统计软件包进行比较。平均比较结果显示,疟疾感染频次的平均值显著增加(p < 0.05)。疟疾感染是最常见的疾病,4月前后发病频率最高,占发病总数的63.83 ~ 67.10%。其次依次为上呼吸道感染(URTI)、消化性溃疡和念珠菌病。疟疾与伤寒、疟疾与尿路感染呈显著正相关(p<0.05)。从问卷分析的结果来看,70.89%的男生组承认每年有1 - 12次疟疾感染,其中43.67%的男生声称曾经感染过伤寒。结果,每年有25.32%的学生旷课1- 30天。这些结果表明,疟疾感染正在增加,除了可能对学生的成绩产生负面影响外,还可能成为发展中经济体的一个巨大的资金流失来源。
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引用次数: 4
The effects of four strains of mycorrhizal fungi and goat manure on fodder production by Moringa oleifera under rain-fed conditions in the Gambia 冈比亚雨养条件下4株菌根真菌和羊粪对辣木饲料生产的影响
Pub Date : 2012-09-01 DOI: 10.5251/ABJNA.2012.3.10.391.399
V. Asaolu, S. Odeyinka
Four myccorhizal fungi strains {Glomus facultative, Glomus mossae, Glomus agregatum and Glomus intraradices } and goat manure were assessed as options to NPK fertilization on fodder production by Moringa oleifera over a 60-day growing period in a randomized complete design. Germination percentages, average plant heights, canopy formation, biomass yields and fodder:stem ratios were monitored. Experimental soil samples' and resulting moringa fodders' contents of N, P and Ca were assessed. No fertilization (P>0.05) effects were observed on germination percentage and plant height at germination. The myccorhizal fungi (except Glomus facultative), NPK and goat manure produced comparable moringa biomass yields, with each treatment producing above 4 tons DM/ha. Fertilization effects (P<0.05) were however observed on plant height at harvest, final canopy formation and the resulting fodder:stem ratios. No significant (P>0.05) effects were observed on the mineral contents of the moringa fodders, while a significant (P<0.05) depletion in available soil P was observed. Two of the investigated mycorrhizal fungi; Glomus agregatum and Glomus mossae, and goat manure applications could be practical alternatives to chemical fertilization in the cultivation of Moringa oleifera for fodder production, with goat manure having the edge in terms of relative availability, cost and nutrient recycling.
采用随机完全设计,评价了4种菌根真菌(兼性Glomus、mossae Glomus、Glomus agregatum和Glomus intraradices)和羊粪作为氮磷钾施肥对辣木饲料生产的影响。监测发芽率、平均株高、冠层形成、生物量产量和料茎比。对试验土壤样品及所得辣木饲料的氮、磷、钙含量进行了评价。不施肥对发芽率和萌发时株高无显著影响(P>0.05)。菌根真菌(兼性球囊菌除外)、氮磷钾和羊粪处理的辣木生物量产量相当,每个处理的DM均在4吨/公顷以上。施肥对辣木饲粮矿质元素含量有显著影响(P<0.05),对土壤速效磷有显著的耗损(P<0.05)。两种被调查的菌根真菌;在辣木饲料种植中,施用聚合球囊菌(Glomus agregatum)、苔藓球囊菌(Glomus mossae)和羊粪可以替代化学施肥,而羊粪在相对可得性、成本和养分循环方面具有优势。
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引用次数: 6
Comparative study of growth and laying performance of indigenous layer guinea fowls (Numida meleagris) from Upper East, Upper West and Northern Regions of Ghana 加纳上东部、上西部和北部地区产蛋珍珠鸡生长和产蛋性能的比较研究
Pub Date : 2012-09-01 DOI: 10.5251/ABJNA.2012.3.9.354.359
A. Agbolosu, G. Teye
An eleven-week experiment to compare the performance of 45 weeks old local layer guinea fowls from Upper East (UER), Upper West (UWR) and Northern (NR) regions of Ghana was conducted at the Animal Science Department farm of the University for Development Studies, Tamale. 243 birds, 86 from UER, 79 from UWR and 78 from NR were used. Birds from each region were divided into four groups and put into four cages each. The diet was made up of 25% layer concentrate, 55% maize and 20% wheat bran. Water was provided ad libitum. Parameters measured were feed intake (FI), body weight, and weight gain, feed conversion efficiency (FCE), mortality, rate of egg laying and fertility. There were no significant differences (P>0.05) in FI and FCE of birds from the three regions. UWR birds were more efficient in feed conversion than birds from UER and NR. Mortality was generally low. Birds from UWR laid more eggs (631) while UER birds recorded the lowest (352 eggs). Egg fertility was high (74%) with NR birds but low in UER (53.6%) and UWR birds (43%). UWR birds exhibited high potential for egg production and FCE than those from the UER and NR. NR birds performed fairly well in egg production and body weight gain. UER birds laid fewer eggs though they had large body size indicating they possess meat production tendencies.
为了比较加纳上东部(UER)、上西部(UWR)和北部(NR)地区45周龄当地蛋鸡的生产性能,在Tamale发展研究大学动物科学系农场进行了为期11周的试验,共243只鸡,其中UER地区86只,UWR地区79只,北部地区78只。每个地区的鸟被分成四组,每组放四个笼子里。饲粮由25%蛋鸡精料、55%玉米和20%麦麸组成。水是免费提供的。测定的参数包括采食量、体重、增重、饲料转化率、死亡率、产蛋率和受精率。3个地区鸟类的FI和FCE差异无统计学意义(P>0.05)。低海拔地区的鸟类比低海拔地区和低海拔地区的鸟类饲料转化率更高,死亡率普遍较低。UWR产蛋最多(631枚),UER产蛋最少(352枚)。NR组产蛋率高(74%),UER组和UWR组产蛋率分别为53.6%和43%。与UER和NR相比,UWR鸟类在产蛋量和FCE方面表现出更高的潜力,而NR鸟类在产蛋量和增重方面表现良好。乌尔鸟虽然体型较大,但下蛋较少,这表明它们具有产肉倾向。
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引用次数: 8
Risks and uncertainties in food production and their implications for extension work in Nigeria 粮食生产中的风险和不确定性及其对尼日利亚推广工作的影响
Pub Date : 2012-09-01 DOI: 10.5251/ABJNA.2012.3.9.345.353
A. Donye, A. Ani
This paper examines the risks and uncertainties in food production and their implications for extension work in Nigeria. It also discusses some of the factors constituting uncertainties that limit agricultural production and productivity. These factors include, inter alia, land, labour, farm implements and inputs, market systems and government policies. On the other hand, weather risks, biological risks, technical risks and economic and social risks were discussed as other circumstances which greatly reduce the values of agricultural produce and products in terms of quality and quantity. Some risk mitigation and coping strategies which were noted as important ways of reducing crop losses include wiping out the sources of infection, use of disease-resistant plants for crop production and seed propagation, practicing good crop rotation and plant association, use of chemical products and growing crops in mixture. Some of the implications noted for extension work require that extension workers should move beyond simply providing farmers with information on food production and standardized technological packages. They should also provide advice and guidance that facilitate communication and coordination among many different stakeholders in natural resource management and market supply chains. Agricultural extension workers should be able to assist farmers in developing a range of agricultural production options. Extension workers and government should strengthen the existing village-level marketing options. They should analyze the marketing seasons, interact with the people involved in marketing and the provision of transportation facilities and thus, advise farmers accordingly. The recommendations made include, among others, the need for the Government of Nigeria to create and maintain the infrastructure required for agricultural development, ensure that research and extension services, input supply and credit arrangements, marketing structures and price system as well as communication and transport networks are all properly put in place.
本文探讨了尼日利亚粮食生产中的风险和不确定性及其对推广工作的影响。它还讨论了构成限制农业生产和生产力的不确定因素的一些因素。这些因素除其他外包括土地、劳动力、农具和投入、市场制度和政府政策。另一方面,天气风险、生物风险、技术风险以及经济和社会风险被视为在质量和数量方面大大降低农产品和产品价值的其他情况。被认为是减少作物损失的重要途径的一些风险缓解和应对战略包括消除感染源、在作物生产和种子繁殖中使用抗病植物、实行良好的作物轮作和植物结合、使用化学产品和混合种植作物。对推广工作所指出的一些影响要求推广工作者不应仅仅向农民提供有关粮食生产的信息和标准化的成套技术。它们还应提供咨询和指导,促进自然资源管理和市场供应链中许多不同利益攸关方之间的沟通和协调。农业推广工作者应当能够协助农民制定一系列农业生产选择。推广工作者和政府应加强现有的村级营销选择。他们应该分析销售季节,与参与营销和提供运输设施的人员进行互动,从而向农民提供相应的建议。提出的建议包括,除其他外,尼日利亚政府需要建立和维持农业发展所需的基础设施,确保研究和推广服务、投入供应和信贷安排、销售结构和价格制度以及通信和运输网络都适当到位。
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引用次数: 4
Field evaluation of three biopesticides for integrated management of major pests of tomato, Solanum lycopersicum L. in Sudan 三种生物农药在苏丹番茄主要害虫综合治理中的田间评价
Pub Date : 2012-09-01 DOI: 10.5251/ABJNA.2012.3.9.340.344
H. El-Shafie, B. Abdelraheem
Two field experiments were conducted at two different locations in Khartoum State, Sudan during the cropping season 2007/2008 to evaluate the efficacy of three biopesticides on the major insects of tomato, (Solanum lycopersicum L.). Treatments applied were NeemAzal ® -T/S (2L/ha), XenTari ® (1kg/ha), Spinosad ® (2L/ha). Sumicidin ® 20% EC was applied at a rate of 0.7L/ha for comparison. Major insect pests studied were whitefly, Bemisia tabaci; aphids, Aphis gossypii and African bollworm (ABW), Helicoverpa armigera. All treatments significantly reduced the populations of the test insect pests. NeemAzal proved to be nearly as effective as Sumicidin. Higher numbers of the green lacewing, Chrysoperla carnea (Steph.) were found in biopesticidestreated and control plots compared with fewer numbers in Sumicidintreated plots. The per cent increase in yield was 32%, 31.2%, 25.3% and 20% in plots treated with NeemAzal, Sumicidin, Xen Tari and Spionsad respectively.
2007/2008年种植季,在苏丹喀土穆州的两个不同地点进行了两项田间试验,以评价三种生物农药对番茄主要昆虫(茄类)的药效。采用NeemAzal®-T/S (2L/ha)、XenTari®(1kg/ha)、Spinosad®(2L/ha)处理。以0.7L/ha的剂量施用20% EC的Sumicidin®进行比较。研究的主要害虫有粉虱、烟粉虱;棉蚜、非洲棉铃虫、棉铃虫。所有处理均显著减少了试验害虫的数量。NeemAzal被证明几乎和Sumicidin一样有效。生物农药处理区和对照区绿草蛉的数量较多,而杀虫剂处理区数量较少。施用NeemAzal、Sumicidin、Xen Tari和Spionsad的地块增产幅度分别为32%、31.2%、25.3%和20%。
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引用次数: 11
Microbiological quality of smoke-dried mangrove oysters (Crassostrea gasar) sold in Port Harcourt, Nigeria 尼日利亚哈科特港出售的烟熏干红树林生蚝(Crassostrea gasar)的微生物质量
Pub Date : 2012-09-01 DOI: 10.5251/ABJNA.2012.3.9.360.364
N. Odu, H. Njoku
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引用次数: 9
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Agriculture and Biology Journal of North America
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