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Nitrate uptake and nitrite efflux in wild type and Li + -R and Na + -R strains of Spirulina platensis under different nitrogen concentration and light- dark condition. 不同氮浓度和明暗条件下野生型和Li + - r、Na + - r螺旋藻对硝酸盐的吸收和亚硝酸盐的外排。
Pub Date : 2012-02-01 DOI: 10.5251/ABJNA.2012.3.2.77.81
N. Sharma, Surendra Singh, Rekha Patel
In the present study we have investigated that 4.0gL -1 NaNO3 concentration is toxic to the growth of wild type Spirulina platensis and its Li + R and Na + R mutant strains. Highest level of extracellular nitrite release was shown by Na + R strain, when grown in 4.0gL -1 NaNO3 concentration followed by Li + R and wild type strain of S. platensis whereas Li + R strain uptake nitrate more rapidly followed by Na + R and wild type strain of S. platensis, when estimated after 8d of incubation. Cultures assimilate nitrate more rapidly in light than in darkness which suggests that nitrate uptake process depend on photosynthetically generated light energy. The nitrate uptake pattern consisted of two distinct phase : initial rapid phase followed by the slower second phase. There is no lag in the initial uptake of nitrate in either of the culture suggesting that rapid phase is not dependent on reduction of nitrate whereas slower phase seemed to be dependent on metabolism. Among these three strains of S. platensis Li + R strain assimilate nitrate more rapidly and fix it into ammonia by sequential action of nitrate reductase and nitrite reductase.
本研究研究了4.0gL -1 NaNO3浓度对野生型螺旋藻及其Li + R和Na + R突变株生长的毒性作用。在4.0gL -1 NaNO3浓度下生长时,Na + R菌株的细胞外亚硝酸盐释放量最高,其次是Li + R和野生型菌株,而在培养8d后,Li + R菌株对硝酸盐的吸收速度最快,其次是Na + R和野生型菌株。培养物在光照条件下比在黑暗条件下吸收硝酸盐的速度更快,这表明硝酸盐的吸收过程依赖于光合作用产生的光能。硝酸盐的吸收模式分为两个不同的阶段:最初的快速阶段和随后的缓慢的第二阶段。在任何一种培养中,硝酸盐的初始吸收都没有滞后,这表明快速阶段不依赖于硝酸盐的还原,而缓慢阶段似乎依赖于代谢。其中,铂氏葡萄球菌Li + R菌株通过硝酸还原酶和亚硝酸盐还原酶的顺序作用,更快地吸收硝酸盐并将其固定到氨中。
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引用次数: 0
Production of specific antiserum against aflatoxin g1 and determination of its efficiency to detect the toxin in wheat and barley grains 黄曲霉毒素g1特异性抗血清的制备及其对小麦和大麦中黄曲霉毒素检测效果的测定
Pub Date : 2012-02-01 DOI: 10.5251/ABJNA.2012.3.2.39.42
Sarah Al Nabwany, A. Alawami
The present study was carried out to produce antiserum for aflatoxin G1 (AFG1) and to use indirect ELISA to detect Aflatoxin in grain samples. Antiserum of AFG1 was produced in New Zealand white rabbits. The results of indirect ELISA showed that the second collection (after 7 days) was the best that contained antibodies and gave the highest values of absorbance. Positive ELISA values were obtained up to dilution of 1:204800 with antiserum of the second collection. On the other hand, the indirect ELISA used to detect aflatoxin G1 in grain showed that 2 wheat samples and 7 barley samples gave a positive reaction with produced antiserum which possessed absorbency values were double that of the normal serum.
本研究旨在制备黄曲霉毒素G1 (AFG1)抗血清,并采用间接ELISA法检测粮食样品中的黄曲霉毒素。用新西兰大白兔制备AFG1抗血清。间接ELISA结果显示,第二次采集(7 d后)抗体含量最高,吸光度最高。第二次采集的抗血清稀释至1:204800时,ELISA结果为阳性。另一方面,用间接ELISA法检测粮食中黄曲霉毒素G1, 2份小麦样品和7份大麦样品的抗血清反应阳性,其吸光度值是正常血清的两倍。
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引用次数: 0
Effect of four different tillage practices on maize performance under rainfed conditions 四种不同耕作方式对旱作条件下玉米生产性能的影响
Pub Date : 2012-01-01 DOI: 10.5251/ABJNA.2012.3.1.25.30
S. Aikins, O. Owusu-Akuoko
An experiment was conducted on Ferric Acrisol under rainfed conditions during the 2010 minor crop growing season in Kumasi, Ghana to determine the effect of different tillage practices on Akposoe maize (Zea mays .L) variety performance. The experiment was arranged in a randomised complete block design with four treatments namely disc ploughing only, disc ploughing followed by disc harrowing, disc harrowing only and No Tillage. Each treatment was replicated three times. At ten weeks after planting, the disc harrowing only plots produced the tallest plant, biggest stem girth, highest number of leaves per plant, and highest leaf area index. On the other hand the No Tillage plots presented the shortest plant, smallest stem girth, lowest number of leaves per plant, and smallest leaf area index. At harvest, the longest root, highest dry matter yield and highest fresh cob weight were obtained from the disc harrowing only plots while the shortest root, lowest dry matter yield, lowest fresh cob weight, lowest dry cob weight, and smallest 1000-seed weight were located in the No Tillage plots. The disc ploughing followed by disc harrowing treatment produced the highest dry cob weight and highest 1000-seed weight. There was no significant difference in Akposoe maize plant growth, dry matter yield and yield components between the disc harrowing only and the disc ploughing followed by disc harrowing treatments. Thus, considering the soil and weather conditions of the experiment, the optimum tillage practice for producing Akposoe maize variety is disc harrowing only.
为了确定不同耕作方式对Akposoe玉米(Zea mays . l)品种生产性能的影响,在2010年加纳库马西(Kumasi)小作物生长季进行了旱作条件下的阿克里索(Ferric Acrisol)试验。试验采用完全随机区组设计,设4个处理,分别为单盘翻耕、单盘翻耕、单盘翻耕和免耕。每个治疗重复三次。在种植后10周,单盘耙地植株最高,茎周长最大,单株叶数最多,叶面积指数最高。免耕样地植株最短,茎周长最小,单株叶数最少,叶面积指数最小。收获时,单盘耙地根系最长、干物质产量最高、鲜穗轴重最高,免耕地根系最短、干物质产量最低、鲜穗轴重最低、干穗轴重最低、千粒重最小。盘耕后盘耙处理的干重和千粒重最高。单纯盘耙与盘耙加盘耙处理对阿克泊索玉米植株生长、干物质产量和产量构成均无显著影响。因此,考虑到试验土壤和气候条件,阿克泊索玉米品种的最佳耕作方式为单盘耙耕。
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引用次数: 50
Preparation and physicochemical characterization of icacina starch citrate – a potential pharmaceutical / industrial starch 一种潜在的药用/工业淀粉——枸橼酸伊卡那淀粉的制备及其理化性质
Pub Date : 2012-01-01 DOI: 10.5251/ABJNA.2012.3.1.11.16
M. Omojola, O. Orishadipe
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引用次数: 11
Effect of four different tillage practices on soil physical properties under cowpea 四种耕作方式对豇豆土壤物理性质的影响
Pub Date : 2012-01-01 DOI: 10.5251/ABJNA.2012.3.1.17.24
S. Aikins, J. Afuakwa
Cowpea (Vigna unguiculata [L.] Walp) is grown by resource poor farmers in Ghana using different tillage practices. Most of these farmers perform tillage operations without being aware of the effect of these operations on soil physical properties and crop responses. A field study was conducted during the 2009 and 2010 major crop growing seasons under rainfed conditions on sandy loam soil (Ferric Acrisol) to compare the effect of different tillage practices on some selected soil physical properties under Asontem cowpea variety. The experiment was arranged in a randomized complete block design with three replications. The treatments consisted of disc ploughing only, disc ploughing followed by disc harrowing, disc harrowing only and no tillage. Compared with the other treatments, the disc ploughing followed by disc harrowing treatment gave the most favourable soil conditions (i.e. lowest soil penetration resistance, lowest dry bulk density, highest soil moisture content, and highest total porosity). The no tillage plots produced the most unfavourable soil conditions (i.e. highest soil penetration resistance, highest dry bulk density, lowest soil moisture content, and lowest total porosity). Therefore, under the soil and weather conditions of the experiment, the best tillage practice identified for Asontem cowpea production is disc ploughing followed by disc harrowing.
豇豆[L];Walp)是由加纳资源贫乏的农民使用不同的耕作方法种植的。这些农民中的大多数在进行耕作作业时没有意识到这些作业对土壤物理性质和作物反应的影响。在2009年和2010年主要作物生长季节,在旱作沙壤土(亚硝酸铁)上进行了田间试验,比较了不同耕作方式对Asontem豇豆品种土壤部分物理性状的影响。试验采用完全随机区组设计,每组3个重复。处理为单盘耕、单盘耕后单盘耙、单盘耙和免耕。与其他处理相比,盘犁后盘耙处理的土壤条件最有利(土壤抗渗透能力最低、干容重最低、土壤含水量最高、总孔隙度最高)。免耕地块的土壤条件最不利(即土壤抗渗透能力最强、干容重最强、土壤含水量最低、总孔隙度最低)。因此,在本试验土壤和气候条件下,确定的最佳耕作方式为盘犁后盘耙。
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引用次数: 56
Further physicochemical characterization of anchomanes difformis starch 异黄酮淀粉的进一步理化性质研究
Pub Date : 2012-01-01 DOI: 10.5251/ABJNA.2012.3.1.31.38
M. Afolayan, M. Omojola
Anchomanes difformis (Blume) Engl. [family ARACEAE] is an herbaceous plant with prickly stem having huge divided leaf and spathe that arise from a horizontal tuber occurring in the forest of West Africa and presently untilized. The tuber of the plant was examined for its starch composition and physicochemical properties. The starch was isolated using 1 % w/v sodium metabisulphite and it gave a yield of about 21 % which is slightly off white in colour. The starch percentage solubility at 85 O C was 17.32 with a swelling power of 9.66 and gelatinization temperature of 72 O C. It has a browning temperature of 257.0 – 268.2 O C, charring temperature of 281.4 – 291.6 O C, water absorption capacity of 71 %, pH of 5.6, foam and emulsion capacities of 3.6 % and 9.17 % respectively. Phytochemical screening of the starch revealed the presence of carbohydrates, terpenoids, saponins and cardiac glycosides while the proximate analysis (in %) was found to be: fat – 1.0, ash – 0.75, crude fibre – 1.6, protein – 0.66, moisture – 6.0, and carbohydrates – 88.9.The paste clarity was determined at 580 nm as a function of the starch concentration. The photomicrograph indicates that the starch granule is generally small sized, not distinct but clustered with size ranging between 1 – 1.5 μm which is a reflection of the parent source. Generally, the values obtained from the physicochemical characterization of anchomanes difformis starch show that it has high potential for industrial applications especially in the food, textile and pharmaceutical industries.
【英】异角鳗[family ARACEAE]是一种草本植物,茎多刺,有巨大的分叶和芽,由一种水平块茎长出,生长在西非的森林中,目前尚未利用。对该植物的块茎进行了淀粉组成和理化性质的检测。用1% w/v的焦亚硫酸钠分离淀粉,得到的淀粉收率约为21%,颜色略偏白。淀粉在85℃下的溶解度为17.32,膨胀力为9.66,糊化温度为72℃,褐变温度为257.0 ~ 268.2℃,炭化温度为281.4 ~ 291.6℃,吸水率为71%,pH值为5.6,泡沫率为3.6%,乳化率为9.17%。淀粉的植物化学筛选显示存在碳水化合物、萜类、皂苷和心糖苷,而近似分析(以%计)发现:脂肪- 1.0,灰分- 0.75,粗纤维- 1.6,蛋白质- 0.66,水分- 6.0,碳水化合物- 88.9。糊状物的清晰度在580 nm处测定,作为淀粉浓度的函数。显微观察结果表明,淀粉颗粒粒径较小,不明显,呈簇状分布,粒径范围在1 ~ 1.5 μm之间,是母源的反映。总的来说,从异绵竹淀粉的理化性质得到的值表明,它具有很高的工业应用潜力,特别是在食品、纺织和制药工业。
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引用次数: 23
Microbial degradation potential of some Ghanaian soils contaminated with diesel oil 受柴油污染的加纳土壤的微生物降解潜力
Pub Date : 2012-01-01 DOI: 10.5251/ABJNA.2012.3.1.1.5
I. Lawson, E. Nartey
The experiment was conducted in the laboratory to investigate the degradation of diesel oil in four soils obtained from semi-deciduous forest (Bekwai and Kokofu series) and savanna (Toje and Nyankpala series) zones with the aim to assess the degrading potential of these soils. The soils were contaminated with diesel oil at 10 g oil /kg soil. The contaminated soils were incubated under room temperature and sampled for total aerobic heterotrophic (TAH) bacterial counts, hydrocarbon utilizing bacterial (HUB) populations, and quantity of diesel oil degraded. The TAH bacteria and HUB counts in all the soils increased in response to diesel oil contamination. The TAH bacteria counts in the forest soils took a longer time to reach their peaks than the savanna soils. The cumulative diesel oil degraded gave an upward trend during the period of study. Between 15 and 40 days after incubation the forest soils (Bekwai and Kokofu) degraded more oil than the savanna soils (Toje and Nyankpala). This study revealed that the main factors responsible for differences in degrading abilities of these soils are presence of large populations of hydrocarbon utilizing bacteria, availability of organic carbon, nitrogen and phosphorus. The study also therefore indicated that the Ghanaian soils used in the present investigation have the potential of degrading diesel oil.
本试验在实验室进行,研究了取自半落叶森林(Bekwai和Kokofu系列)和稀树草原(Toje和Nyankpala系列)的四种土壤对柴油的降解,目的是评估这些土壤的降解潜力。以10 g /kg的柴油污染土壤。对污染土壤进行室温培养,取样检测好氧异养细菌总数(TAH)、烃类利用细菌数量(HUB)和柴油降解量。所有土壤的TAH细菌和HUB数量均随柴油污染而增加。森林土壤中TAH细菌数量达到峰值所需的时间比稀树草原土壤长。研究期间柴油累计降解量呈上升趋势。在孵化后15至40天,森林土壤(Bekwai和Kokofu)比稀树草原土壤(Toje和Nyankpala)降解更多的油。研究表明,影响土壤降解能力差异的主要因素是大量烃类利用菌群的存在以及有机碳、氮、磷的有效性。因此,这项研究还表明,在本研究中使用的加纳土壤具有降解柴油的潜力。
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引用次数: 12
Identification of suitable parents and temperatures for breeding Potato virus Y (PVY) and Potato virus X (PVX) resistant potatoes 马铃薯Y病毒(PVY)和X病毒(PVX)抗性马铃薯适宜亲本和适宜温度的鉴定
Pub Date : 2011-12-01 DOI: 10.5251/ABJNA.2011.2.12.1409.1415
K. Njoroge, J. Onditi, I. S. Shibairo
Mixed infection of Potato virus Y (PVY) and Potato virus X (PVX) together with other potato viruses have the potential of causing yield losses of up to 80 % in the major varieties grown in Kenya. In search for suitable resistant parents and a favourable temperature range for cross breeding, seven virus resistant potato genotypes from International Potato Centre (CIP), Lima Peru were test crossed (progeny tested) with one local PVY and PVX susceptible cultivar (Tigoni) under low (11-18 ◦ C), medium (18-27 ◦ C) and high temperatures (28-34 ◦ C) at the Kenya Agricultural Research Institute (KARI), Tigoni. Low temperature range of (11-18 ◦ C) with the highest percentage (43%) of successful crosses was identified as the most favourable for cross breeding. Among the seven CIP clones tested, CIP395196.4 gave the highest percentage (98 %) of resistant progenies and was found to have multiplex Ry Ry Ry ry / Ry Ry Ry Ry genes for PVY and PVX resistance. This type of parent was the most suitable for cross breeding because it produced significantly (P=0.05) 100% resistant progenies when crossed with a susceptible cultivar hence eliminating the need (cost and labour) for preliminary seedling screening.
马铃薯Y病毒(PVY)和马铃薯X病毒(PVX)与其他马铃薯病毒的混合感染有可能导致肯尼亚种植的主要品种的产量损失高达80%。为了寻找合适的抗性亲本和适宜的杂交育种温度范围,在Tigoni肯尼亚农业研究所(KARI),将来自秘鲁利马国际马铃薯中心(CIP)的7个病毒抗性马铃薯基因型与一个当地PVY和PVX易感品种(Tigoni)在低温(11-18℃)、中温(18-27℃)和高温(28-34℃)下进行杂交(后代测试)。在低温(11-18℃)范围内杂交成功率最高(43%),最有利于杂交育种。在7个CIP无性系中,CIP395196.4的抗性子代比例最高(98%),具有多重的Ry Ry Ry Ry Ry / Ry Ry Ry基因对PVY和PVX的抗性。这种亲本最适合杂交育种,因为它与感病品种杂交时产生100%抗性的后代显著(P=0.05),从而消除了初步筛选幼苗的需要(成本和劳动力)。
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引用次数: 1
Effect of egg size and strain on growth performance of cockerel 蛋大小和品系对公鸡生长性能的影响
Pub Date : 2011-12-01 DOI: 10.5251/ABJNA.2011.2.12.1445.1453
L. T. Egbeyale, S. Abiola
This study was conducted to determine the effect of egg size and strain on growth performance of cockerel chicks obtained from Dominant Black (DB) and Yaffa Brown (YB) strains of pullet. A total number of two hundred and seventy day-old cockerels hatched from three different egg sizes (i.e 45 chicks from small, medium and large egg sizes for each of DB and YB) were used for this study. Data obtained were subjected to analysis of variance in a 2 x 3 factorial experimental layout. The initial weight of the chicks was significantly (P<0.05) influenced by the egg size. Final weight and average daily weight gain were the only growth parameters affected (P<0.05) by egg size till the end of starter phase. The effect of strain on all the growth parameters measured were not significantly (P>0.05) different. At the growing phase, feed intake decreased significantly (P<0.05) with increase in egg size. It was concluded that setting of medium and large egg sizes could only be beneficial if the target was to sell chicks of the two strains at the end of chick phase.
本试验旨在研究蛋大小和品系对优势黑(DB)和雅法布朗(YB)两种雏鸡生长性能的影响。本研究共选用三种不同蛋尺寸(即DB和YB各45只小、中、大蛋尺寸的雏鸡)孵化的270日龄公鸡。获得的数据在2 × 3的析因实验布局中进行方差分析。雏鸡初始体重差异显著(P0.05)。生长期采食量随蛋大小的增加而显著降低(P<0.05)。综上所述,如果目标是在鸡龄后期销售这两个品系的鸡,则设置中、大鸡蛋尺寸是有益的。
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引用次数: 9
Distribution of injected pesticides in date palm trees 注射农药在枣椰树中的分布
Pub Date : 2011-12-01 DOI: 10.5251/ABJNA.2011.2.12.1416.1426
Adnan Al Samarrie, A. Akela
The insecticides Rogodial (dimethoate + phenthoate), Actellic (primiphos-methyl), Salut {(chlorpyrifos-ethyl) + dimethoate}, Reldan (chlorpyrifos-methyl) and Karate (lambda-cyhalothrin) translocation and movement in date palm trees were evaluated. It found that the systemic and non-systemic insecticides moved out of injection pores. The injected pesticides detected 10 Days post Injection (DPI) at one meter above the injection point in the same and opposite side of injection pore. The five individual injected pesticides distributed downward at concentrations of 0.01, 0.011, 0.009, 0.011 and 0.009 mg/kg of actellic, salut, karate, reldan and rogodial respectively. At 20 DPI all studied pesticides were detected in the pith at 2m above the injection side either individually or mixture injected pesticides, Similarly, the pesticides were found to be distributed downward at concentrations of 0.042, 0.036, 0.005, 0.019 and 0.025 mg/kg of actellic, salut, karate, reldan and rogodial respectively. The residues of four insecticides detected in dates 100 DPI, the insecticide residue levels of karate in the dates were 0.0034 & 0.019 of mixed and individual injected pesticides respectively which were the highest levels at 100DPI.
测定了杀虫剂Rogodial(乐果+苯多酸盐)、Actellic(原生态磷-甲基)、Salut(毒死蜱-乙基)+乐果}、Reldan(毒死蜱-甲基)和Karate(氯氟氰菊酯)在枣椰树上的迁移和移动情况。研究发现,全身性和非全身性杀虫剂从注射孔中移出。注射后10 d,在注射点上方1 m处注射孔同侧和对侧检测到注射后10 d的农药。5种农药分别以0.01、0.011、0.009、0.011和0.009 mg/kg的浓度向下分布。在20 DPI时,在注射侧上方2m的髓内均检测到所研究的农药,分别为单独或混合注射农药,分别以0.042、0.036、0.005、0.019和0.025 mg/kg的浓度向下分布。在100DPI的枣子中检测到4种杀虫剂的残留量,其中空手道在混合和单独注射时的残留量分别为0.0034和0.019,在100DPI时残留量最高。
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引用次数: 13
期刊
Agriculture and Biology Journal of North America
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