Pub Date : 1977-01-01DOI: 10.1016/S0005-8165(77)80082-6
W. Roggendorf , U. Gross
In connection with various diseases, spindle-shaped or roundish 2-15 μm large corpuscles with a yellowish-green, partly brown color are found in phagocytic cells of lymph nodes of various localisation. There are three groups according to staining and ultrastructural characteristics.
1.
Strongly Fe+++ positive corpucles only,
2.
corpuscles with a positive PAS-reaction, Ziehl-Neelsen and Sudan black B stain and slight iron adsorption,
3.
particles with irregular stainability.
Ultrastructurally, the spindle bodies of Group 1 have a uniform membrane, double in some sections and a narrow, finely granular rim, in which 50 ä large iron micelles are stored. The electrondense center contains structures which may correspond to remainders of cell organelles. The similarly shaped particles of Group 2 have a homogeneous, electron-dense center and sometimes half-moon-shaped, homogeneous, less dense appositions. A dense round center with finely honey-combed translucencies and marginal vacuoles characterizes the irregularly shaped, in part spindly corpuscles of Group 3. The energy-dispersion X-ray analysis indicates only iron for the particles of Group 1. A relation of the corpuscles to specific diseases, especially to sarcoidosis and certain therapeutic measures, is not evident.
Bei verschiedenen Grundkrankheiten finden sich in phagozytierenden Zellen von Lymphknoten unterschiedlicher Lokalisation spindelförmige oder rundliche 10 × 2 μm groβe Körperchen mit gelblich-grüner, teilweise brauner Eigenfärbe. Nach färberischem und ultrastrukturellem Verhalten gibt es 3 Gruppen.
1.
Körperchen mit ausschlieβlich stark positivem Fe+++-Nachweis,
2.
Körperchen mit positiver PAS-Reaktion, Färbung nach Ziehl-Neelsen, Sudanschwarz B und geringer Eisenadsorption,
3.
Partikel mit uneinheitlicher Färbbarkeit.
Ultrastrukturell besitzen die Spindelkörper der 1. Gruppe eine abschnittsweise verdoppelte Einheitsmembran, einen schmalen feingranulären Randsaum, in dem 50 ä groβe Eisenmizellen eingelagert sind. Im elektronendichten, offenbar agglomerierten Zentrum sind vereinzelt Strukturen erkennbar, die Resten von Zellorganellen entsprechen können. Die ähnlich geformten Partikel der 2. Gruppe besitzen ein homogenes elektronendichtes Zentrum und gelegentlich halbmondförmige, weniger dichte, homogene Appositionen. Die unregelmäβig geformten, teilweise spindeligen Körperchen der 3. Gruppe kennzeichnet ein rundliches dichtes Zentrum mit feinwabigen Aufhellungen und randständigen Vakuolen. Die energiedispersive Röntgen-Mikroanalyse weist für die Partikel der 1. Gruppe ausschlieβlich Eisen nach. Eine bestimmte Beziehung der Körperchen zu Grundkrankheiten, insbesondere zu Sarkoid
在不同部位的淋巴结吞噬细胞中可见纺锤状或圆形的2-15 μm大的小体，呈黄绿色，部分呈褐色，与各种疾病有关。根据染色和超微结构特征可分为三组。只有强fe++ +正粒子，2。2 . pas反应阳性的小体，Ziehl-Neelsen和苏丹黑B染色和轻微的铁吸附。具有不规则污渍的颗粒。在超微结构上，第1组的纺锤体具有均匀的膜，某些部分为双层，边缘呈细粒状，其中储存着50个ä大的铁胶束。电子致密中心包含的结构可能与细胞器的残留物相对应。类似形状的第2族粒子有一个均匀的、电子密度大的中心，有时也有半月形的、均匀的、密度较小的对偶。密集的圆形中心有精细的蜂窝状半透明和边缘的液泡，这是不规则形状的特征，部分是第3组的细长小体。能量色散x射线分析表明，第1族粒子只有铁。小体与特定疾病的关系，特别是与结节病和某些治疗措施的关系并不明显。Bei verschiedenen Grundkrankheiten finden sich in phagozytierenden Zellen von Lymphknoten unterschiedlicher Lokalisation spindelförmige oder rundliche 10 × 2 μm groβe Körperchen mit gelblich- gr， teilweise bruner Eigenfärbe。Nach färberischem and ultrastrukturellem Verhalten gibt . 3 Gruppen.1。Körperchen mit ausschlieβlich stark positivem fe++ +-Nachweis,2。2 . Körperchen mit positive pas - reaction, Färbung nach Ziehl-Neelsen, Sudanschwarz B and geringer eisen吸附，Färbung粒子粒子会产生uneinheitlich Färbbarkeit。超冲击微球成形模具Spindelkörper der 1。Gruppe e . abschnittsweise verdoppelte . eisenheitsembran, einenschmalen feingranulären Randsaum, in dem 50 ä groβe . eisenmizeleningelagerne sind。1 .在电子技术方面，研究人员在电子技术方面取得了巨大的进步，在电子技术方面取得了巨大的进步。Die ähnlich geformten Partikel der 2。Gruppe besitzen ein homogenes elektronendichtes Zentrum and geelegentlich halbmondförmige, weniger dichte, homogene Appositionen。模具unregelmäβig geformten, teilwise spindeligen Körperchen der 3。kennzeichnet在rundliches方面的研究发现，该研究中心的研究对象是女性和女性。Die能量色散Röntgen-Mikroanalyse weist f r Die Partikel der 1。ausschlieβlich eisennach集团。我最喜欢的是金色金色Körperchen金色金色金色，最喜欢的是萨科奇金色金色，最喜欢的是治疗金色金色金色金色金色。
{"title":"Ultrastructural and histochemical investigations of spindle-shaped corpuscles in phagocytic cells of lymph nodes","authors":"W. Roggendorf , U. Gross","doi":"10.1016/S0005-8165(77)80082-6","DOIUrl":"10.1016/S0005-8165(77)80082-6","url":null,"abstract":"<div><p>In connection with various diseases, spindle-shaped or roundish 2-15 μm large corpuscles with a yellowish-green, partly brown color are found in phagocytic cells of lymph nodes of various localisation. There are three groups according to staining and ultrastructural characteristics.</p><ul><li><span>1.</span><span><p>Strongly Fe<sup>+++</sup> positive corpucles only,</p></span></li><li><span>2.</span><span><p>corpuscles with a positive PAS-reaction, Ziehl-Neelsen and Sudan black B stain and slight iron adsorption,</p></span></li><li><span>3.</span><span><p>particles with irregular stainability.</p></span></li></ul><p>Ultrastructurally, the spindle bodies of Group 1 have a uniform membrane, double in some sections and a narrow, finely granular rim, in which 50 ä large iron micelles are stored. The electrondense center contains structures which may correspond to remainders of cell organelles. The similarly shaped particles of Group 2 have a homogeneous, electron-dense center and sometimes half-moon-shaped, homogeneous, less dense appositions. A dense round center with finely honey-combed translucencies and marginal vacuoles characterizes the irregularly shaped, in part spindly corpuscles of Group 3. The energy-dispersion X-ray analysis indicates only iron for the particles of Group 1. A relation of the corpuscles to specific diseases, especially to sarcoidosis and certain therapeutic measures, is not evident.</p></div><div><p>Bei verschiedenen Grundkrankheiten finden sich in phagozytierenden Zellen von Lymphknoten unterschiedlicher Lokalisation spindelförmige oder rundliche 10 × 2 μm groβe Körperchen mit gelblich-grüner, teilweise brauner Eigenfärbe. Nach färberischem und ultrastrukturellem Verhalten gibt es 3 Gruppen.</p><ul><li><span>1.</span><span><p>Körperchen mit ausschlieβlich stark positivem Fe<sup>+++</sup>-Nachweis,</p></span></li><li><span>2.</span><span><p>Körperchen mit positiver PAS-Reaktion, Färbung nach Ziehl-Neelsen, Sudanschwarz B und geringer Eisenadsorption,</p></span></li><li><span>3.</span><span><p>Partikel mit uneinheitlicher Färbbarkeit.</p></span></li></ul><p>Ultrastrukturell besitzen die Spindelkörper der 1. Gruppe eine abschnittsweise verdoppelte Einheitsmembran, einen schmalen feingranulären Randsaum, in dem 50 ä groβe Eisenmizellen eingelagert sind. Im elektronendichten, offenbar agglomerierten Zentrum sind vereinzelt Strukturen erkennbar, die Resten von Zellorganellen entsprechen können. Die ähnlich geformten Partikel der 2. Gruppe besitzen ein homogenes elektronendichtes Zentrum und gelegentlich halbmondförmige, weniger dichte, homogene Appositionen. Die unregelmäβig geformten, teilweise spindeligen Körperchen der 3. Gruppe kennzeichnet ein rundliches dichtes Zentrum mit feinwabigen Aufhellungen und randständigen Vakuolen. Die energiedispersive Röntgen-Mikroanalyse weist für die Partikel der 1. Gruppe ausschlieβlich Eisen nach. Eine bestimmte Beziehung der Körperchen zu Grundkrankheiten, insbesondere zu Sarkoid","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"161 3","pages":"Pages 267-282"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-8165(77)80082-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11296924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1976-12-01DOI: 10.1016/S0005-8165(76)80031-5
G.E. Petrzilka , H.E. Schroeder
In the present study, stereologic procedures are employed to estimate quantitatively the composition of chronic connective tissue infiltrates characterizing various oral mucous membrane diseases. One case each of benign mucous membrane pemphigoid (BMMP) and reticular lichen planus (RLP) served as examples to demonstrate the applicability of this methodology. In both lesions which are described clinically, histologically and on the ultrastructural level and which display a markedly different infiltrate composition, fibroblasts were affected and either proliferated (BMMP) or became cytopathologically altered (RLP). The quantitative data presented, besides providing new information, argue in favour of the stereologic approach.
In der vorliegenden Arbeit wird versucht, stereologische Methodik erstmals dazu zu verwenden, quantitativ die Zusammensetzung von chronischen Bindegewebsinfiltraten zu schätzen, welche verschiedenste Mundschleimhauterkrankungen charakterisieren. Um die Verwendbarkeit dieser Methodik zu demonstrieren, wurden je 1 Fall eines gutartigen Schleimhautpemphigoids (BMMP) und eines Lichen planus reticularis (RLP) klinisch, histologisch, ultrastrukturell und quantitativ morphometrisch untersucht. In beiden Läsionen, die eine sehr verschiedene Infiltratzusammensetzung aufwiesen, waren die ortsständigen Fibroblasten entweder proliferierend (BMMP) oder zytopathologisch (RLP) vom Infiltrat betroffen. Die quantitativen Daten, welche für beide Läsionstypen neue Information erbrachten, attestieren die Brauchbarkeit und den Wert einer stereologischen Analyse von chronisch entzündlichen Bindegewebsinfiltraten.
在本研究中，采用立体学方法定量估计慢性结缔组织浸润的组成，表征各种口腔粘膜疾病。以良性粘膜类天疱疮(BMMP)和网状扁平苔藓(RLP)各1例为例，证明了该方法的适用性。这两种病变在临床、组织学和超微结构水平上都有描述，浸润成分明显不同，成纤维细胞受到影响，要么增殖(BMMP)，要么发生细胞病理学改变(RLP)。所提供的定量数据除了提供新的信息外，还支持立体学方法。在der vorligenden arbetwirsucht，立体学方法上，定量上，在时间上，在时间上，在时间上，在时间上，在时间上，在特征上。研究结果表明:1 .研究对象为类癣癣(BMMP)和网状扁平地衣(RLP)，进行了组织学、超微结构和定量形态学分析。在北京Läsionen, die eine sehr verschiedene浸润性浸润性浸润性浸润性浸润性浸润性浸润性浸润性增生(BMMP)和细胞病理学(RLP)浸润性浸润性浸润。Die quantitiven Daten, welche fgr beide Läsionstypen neue Information erbrachten, attestien Die Brauchbarkeit and den Wert einer stereologischen Analyse von chronisch entzndlichen Bindegewebsinfiltraten。
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Pub Date : 1976-12-01DOI: 10.1016/S0005-8165(76)80172-2
M. Balázs
Electron microscopic studies of a benign hepatoma in a 31-year-old women who was on contraceptive pills are presented. The electron microscopic picture showed highly differentiated liver cells with regularly developed bile canaliculi. The mitochondrial polymorphism, the formation of paracristalloids and the appearance of giant mitochondria were striking. The main change was the occurence of the numerous capillaries of varying caliber which were highly differentiated and formed an integral part of the tumor. On the basis of these finding the tumor is regarded as a benign mixed tumor. The well developed capillary system explaines the arteriographic characteristics and the frequent bleeding complications noted in the literature.
Elektronenmikroskopische Untersuchungen eines gutartigen Hepatoms einer 31jährigen, mit oralen Antikonzeptiva behandelten Patientin werden durchgeführt. Die elektronenmikroskopischen Bilder zeigen reife Leberzellen mit regelmäßig entwickelten Gallenkanälchen. Der Polymorphismus der Mitochondria ist auffällig. Hauptbefunde sind: zahlreiche verschieden große Kapillaren, die gut entwickelt sind und einen fundamentalen Teil des Tumors bilden. Aufgrund dessen wird die Geschwulst für einen gutartigen Mischtumor gehalten. Das reichliche Kapillarsystem erklärt die arteriographische Charakteristik und die Blutungsneigung des Turmorgewebes.
电镜研究良性肝癌在一个31岁的妇女谁是避孕药提出。电镜显示肝细胞高度分化，胆管发育规律。线粒体的多态性、拟蝗体的形成和巨大线粒体的出现是显著的。主要的变化是出现大量不同口径的毛细血管，这些毛细血管高度分化并形成肿瘤的组成部分。根据这些发现，认为该肿瘤为良性混合性肿瘤。发达的毛细血管系统解释了文献中提到的动脉造影特征和频繁出血并发症。电子微生物学研究与临床应用研究与临床应用研究与临床应用研究[j];Die elektronenmikroskopischen Bilder zeigen reife Leberzellen mit regelmäßig entwickelten Gallenkanälchen。Der Polymorphismus Der线粒体ist auffällig。haupthebefunde sind: zahlreiche versschieden große Kapillaren, die gut entwickelt sind and einen fundamentalen Teil des Tumors bilden。[au:] [au:] [au:] [au:]血管血管学:血管血管学与血管血管学。
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Pub Date : 1976-12-01DOI: 10.1016/S0005-8165(76)80168-0
H. Knecht
Introduction
Many recent publications have demonstrated that the cryptorchid testicle (and, to a lesser extent, the descended partner) are progressively injured from the second year of life onwards. Do these injuries occur in an organ which has been healthy up to this time or are they superimposed on a structurally abnormal testicle? In order to answer this, parts of cryptorchid testicles, of the descended partners, and of normal testicles were compared by histological examination of serial sections.
Material and Methods
Parts of four testes from children aged 4–7 months (2 specimens obtained by biopsy and 2 from autoptic material) and parts of four testes from children 1½ years old (2 obtained by biopsy and 2 from autoptic material) were examined. The biopsies were fixed in Stieve's fixative. Tissue samples from clinically healthy children who had died suddenly were fixed in 4% formalin. The tissue was embedded in paraffin and sectioned serially; 6 μn sections were stained with HE.
The spermatogonia in each cross-section and in each oblique section of a same tubule were counted and the counts of the latter were adjusted to a cross-section 50–60 μn in diameter. This counting technique did not alter the density of spermatogonia. The graphs present data on the density of spermatogonia through the lengths of the tubules examined and demonstrate tubular branching and blind ends.
In the first year of life the cryptorchid testis and its descended partner showed repeated long sections lacking spermatogonia in the same tubule, whereas in normal testes the spermatogonia were more evenly distributed. The cryptorchid testis showed increased tubule branching in the areas examined.
In the second year of life the tubules of the cryptorchid testis and its descended partner manifest areas free of germ cells, increased branching, and blind ends. The cryptorchid testis also had a tubule completely free of spermatogonia. The germ cell-free parts were always associated with a smaller tubule diameter than normal. The normal testes did not disclose increased branching or spermatogonium-free areas within similar lengths of tubules and showed an even distribution of spermatogonia.
Discussion
The different distribution of spermatogonia within the tubules and the increased branching of the tubules in cryptorchid testes indicate a previous disturbance of testis development.
Anhand ausgesuchter Fälle wurden die Spermatogonienverteilung innerhalb längerer Tubulusabschnitte und der räumliche Aufbau tubulärer Strukturen bei kryptorchen Hoden, ihren deszendierten Partnern sowie bei Normalhoden in Serienschnitten vergleichend untersucht. Im ersten Lebensjahr finden sich Unterschiede in der Art der Spermatogonienverteilung und im Auftreten von tubulären Verzweigungen. Längere spermatogonienfreie Abschnitte im gleichen Tubulus wiederholen sich sowohl beim kryptorchen Hoden als auch
许多最近的出版物表明，从生命的第二年开始，隐睾(以及较小程度上的后代伴侣)逐渐受到损伤。这些损伤是发生在一个一直健康的器官上还是叠加在一个结构异常的睾丸上?为了回答这个问题，我们通过连续切片的组织学检查比较了隐睾、降伴和正常睾丸的部分。材料与方法对4 ~ 7月龄儿童4例睾丸(活检2例，自噬材料2例)和1岁半儿童4例睾丸(活检2例，自噬材料2例)进行部分检测。活组织切片固定在steve的固定物中。将突然死亡的临床健康儿童的组织样本固定在4%福尔马林中。组织石蜡包埋，连续切片;HE染色6 μn切片。对同一小管各横截面和斜截面的精原细胞计数，斜截面的精原细胞计数调整为直径50 ~ 60 μn的横截面。这种计数技术没有改变精原细胞的密度。图中显示了精原细胞密度的数据，通过所检查的小管的长度显示了小管分支和盲端。在生命的第一年，隐睾及其后代伴侣在同一小管中反复出现缺乏精原细胞的长段，而在正常睾丸中，精原细胞分布更均匀。隐睾在检查区域显示小管分支增加。在生命的第二年，隐睾的小管和它的后代伴侣表现出没有生殖细胞的区域，分支增加，和盲端。隐睾也有一个完全没有精原细胞的小管。无生殖细胞部分的小管直径总是比正常的小。正常睾丸在相同长度的小管内未发现分支增加或无精原细胞区域，且精原细胞分布均匀。精原细胞在小管内的不同分布和隐睾小管分支的增加表明先前睾丸发育受到干扰。Anhand ausgesuchter Fälle wurden die spermatgoonienvereilung innerhalb längerer输卵管和输卵管在räumliche Aufbau tubulärer Strukturen bei kryptorchen Hoden, ihren deszendierten partner sowie bei Normalhoden in Serienschnitten vergleichend untersuht。在研究精子发育发育的过程中，研究精子发育发育的过程和研究精子发育的过程。Längere精原体自由Abschnitte im gleichen Tubulus wiederholen，因此，世界上最重要的是，kryptorchen Hoden也最重要的是，最重要的是，合作伙伴。正常输卵管发育与正常输卵管发育的关系Länge与正常输卵管发育的关系。北京kryptorchen Hoden finden sidem viele Verzweigungen augenem Raum。[1]〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕〔1〕[3][1][1][1][1][1][1][1][1][1]。Außerdem zeigen der cryptorche Hoden and sein Partner tubuläre Verzweigungen innerhalb von Distanzen, welche bem Normalhoden verzweigungsfrei sind。Die Unterschiede der Tubuli hinsichtlich spermatogoniverilung and räumlicher Anordnung - insbesonere zwischen kryptorchen Hoden and Normalhoden - innerhalb der ersten zwei lebenjahre lassen eine anlagemäßige Störung bei Kryptorchismus als wahrscheinlich annehmen。
{"title":"Tubulusstruktur und Keimzellverteilung in frühkindlichen kryptorchen und normalen Hoden","authors":"H. Knecht","doi":"10.1016/S0005-8165(76)80168-0","DOIUrl":"10.1016/S0005-8165(76)80168-0","url":null,"abstract":"<div><h3>Introduction</h3><p>Many recent publications have demonstrated that the cryptorchid testicle (and, to a lesser extent, the descended partner) are progressively injured from the second year of life onwards. Do these injuries occur in an organ which has been healthy up to this time or are they superimposed on a structurally abnormal testicle? In order to answer this, parts of cryptorchid testicles, of the descended partners, and of normal testicles were compared by histological examination of serial sections.</p></div><div><h3>Material and Methods</h3><p>Parts of four testes from children aged 4–7 months (2 specimens obtained by biopsy and 2 from autoptic material) and parts of four testes from children 1½ years old (2 obtained by biopsy and 2 from autoptic material) were examined. The biopsies were fixed in Stieve's fixative. Tissue samples from clinically healthy children who had died suddenly were fixed in 4% formalin. The tissue was embedded in paraffin and sectioned serially; 6 μn sections were stained with HE.</p><p>The spermatogonia in each cross-section and in each oblique section of a same tubule were counted and the counts of the latter were adjusted to a cross-section 50–60 μn in diameter. This counting technique did not alter the density of spermatogonia. The graphs present data on the density of spermatogonia through the lengths of the tubules examined and demonstrate tubular branching and blind ends.</p><p>In the first year of life the cryptorchid testis and its descended partner showed repeated long sections lacking spermatogonia in the same tubule, whereas in normal testes the spermatogonia were more evenly distributed. The cryptorchid testis showed increased tubule branching in the areas examined.</p><p>In the second year of life the tubules of the cryptorchid testis and its descended partner manifest areas free of germ cells, increased branching, and blind ends. The cryptorchid testis also had a tubule completely free of spermatogonia. The germ cell-free parts were always associated with a smaller tubule diameter than normal. The normal testes did not disclose increased branching or spermatogonium-free areas within similar lengths of tubules and showed an even distribution of spermatogonia.</p></div><div><h3>Discussion</h3><p>The different distribution of spermatogonia within the tubules and the increased branching of the tubules in cryptorchid testes indicate a previous disturbance of testis development.</p></div><div><p>Anhand ausgesuchter Fälle wurden die Spermatogonienverteilung innerhalb längerer Tubulusabschnitte und der räumliche Aufbau tubulärer Strukturen bei kryptorchen Hoden, ihren deszendierten Partnern sowie bei Normalhoden in Serienschnitten vergleichend untersucht. Im ersten Lebensjahr finden sich Unterschiede in der Art der Spermatogonienverteilung und im Auftreten von tubulären Verzweigungen. Längere spermatogonienfreie Abschnitte im gleichen Tubulus wiederholen sich sowohl beim kryptorchen Hoden als auch","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"159 3","pages":"Pages 249-270"},"PeriodicalIF":0.0,"publicationDate":"1976-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-8165(76)80168-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"55611883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Unlabelled: During the last 3 decades several authors have found in tumour-bearing animals an increase of synthesis and content of DNA in various organs which were free from neoplastic cells (Griffin, 1957;Kelly and Jones, 1950; Morgan and Cameron, 1973; Cerecedo et al., 1951; Lombardo et al., 1952). 5-iodo-2'-deoxyuridine (IUdR) is a thymidine analogue and specifically incorporated into DNA. When it is labelled with 125I or 131I it permits to reinvestigate these findings by measuring the rate of precursor incorporation into DNA and the rate of loss of labelled DNA in the living animal by means of counting the gamma emission from the incorporated iodine isotopes. In this paper, therefore, an attempt is made to analyse the DNA turnover in the whole body of liver tumour-bearing mice.
Material and methods: 5-iodo-2'-deoxyuridine (IUdR) labelled with 125iodine was used as DNA precursor. It is a thymidine analogue 5% of which is specifically incorporated into the DNA of those proliferating cells which are in the phase of DNA synthesis at the moment of tracer application. Non-incorporated IUdR (about 95% of the injected amount) is rapidly degraded and excreted within 24 hours. The tracer remains bound to the cellular DNA druing the life span of the labelled cells. After cell death only about 5% of IUdR from DNA breakdown is reutilized. 125I has a half live of 60 days and therefore allows, over periods of weeks, external measurements of the DNA turnover in the living animal without disturbing the physiological environment. The measured loss of DNA-bound 125I reflects almost exclusively the turnover of the labelled cells. Female albino NMRI mice, 2 months old, bearing sarcoma-180 implanted into the right hind leg were intraveneously injected with 2 muCi 125I-UdR. At the time of injection, the tumour had reached in one group of mice an average volume of about 25 mm3 and in another group an average volume of nearly 850 mm3. When implanted into subcutaneous tissue sarcoma-180 rarely produces metastases in parenchymal organs, never in the spleen and--within the first 30 days after implantation--only in ca. 10% of the animals a small metastasis in a single lymphnode (Deodhar and Crile, 1969; Franchi et al., 1968). Whole body measurements were carried out immediately after tracer injection and then daily during the first week and every second or third day in the following 2 weeks in a NaI well counter with a single channel pulse height analyser. The tumour activity was also determined in vivo by a special counting device.
Results: In the normal mouse 4 to 6% of injected 125I-UdR is retained in the whole body 24 hours after tracer injection. During the following five days the 125I activity rapidly declines to 0.8% of that of day O immediately after injection. Thereafter the rate of loss of activity greatly diminishes (Fig. I). The first component of the turnover curve reflects an average daily ce
未标记:在过去的30年里，几位作者发现，在没有肿瘤细胞的动物体内，各种器官中DNA的合成和含量都有所增加(Griffin, 1957;Kelly和Jones, 1950;摩根和卡梅隆，1973年;Cerecedo等，1951;Lombardo et al.， 1952)。5-碘-2'-脱氧尿嘧啶(IUdR)是一种胸腺嘧啶类似物，并特异性地结合到DNA中。当它被标记为125I或131I时，它允许通过测量前体并入DNA的速率和标记DNA在活动物中的损失率来重新研究这些发现，方法是计算并入碘同位素的伽马辐射。因此，本文试图分析肝癌小鼠全身的DNA转换。材料与方法:以5-碘-2'-脱氧尿苷(IUdR)为DNA前体，125碘标记。它是一种胸腺嘧啶类似物，5%的胸腺嘧啶在示踪剂应用时被特异性地结合到那些处于DNA合成阶段的增殖细胞的DNA中。未掺入的IUdR(约占注射量的95%)在24小时内迅速降解并排出体外。示踪剂在标记细胞的整个生命周期内保持与细胞DNA结合。细胞死亡后，DNA分解产生的IUdR只有约5%被重新利用。125I的半衰期为60天，因此可以在几周内对活体动物的DNA周转进行外部测量，而不会干扰生理环境。测量的dna结合125I的损失几乎完全反映了标记细胞的周转。选取2月龄右后腿植入180肉瘤的雌性白化NMRI小鼠，静脉注射2 muCi 125I-UdR。注射时，一组小鼠的肿瘤平均体积约为25毫米，另一组小鼠的肿瘤平均体积接近850毫米。当植入皮下组织时，肉瘤-180很少在实质器官发生转移，从未在脾脏发生转移，并且在植入后的前30天内，只有约10%的动物在单个淋巴结发生小转移(Deodhar和Crile, 1969;Franchi et al.， 1968)。在注射示踪剂后立即进行全身测量，然后在第一周内每天进行一次，在接下来的两周内每隔第二天或第三天进行一次，使用带有单通道脉冲高度分析仪的NaI井计数器。肿瘤活性也通过一种特殊的计数装置在体内测定。结果:示踪剂注射24小时后，正常小鼠体内仍有4% ~ 6%的125I-UdR残留。在接下来的5天内，125I活性迅速下降至注射后第0天的0.8%。此后，活性损失率大大降低(图1)。周转率曲线的第一个分量反映了平均每天约30%的细胞损失率，涉及约90%的合并活性……
{"title":"[DNA Turnover in the Whole Body of Tumour Bearing Mice (author's transl)].","authors":"P H Kronenberger, W Porschen, L E Feinendegen","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Unlabelled: </strong>During the last 3 decades several authors have found in tumour-bearing animals an increase of synthesis and content of DNA in various organs which were free from neoplastic cells (Griffin, 1957;Kelly and Jones, 1950; Morgan and Cameron, 1973; Cerecedo et al., 1951; Lombardo et al., 1952). 5-iodo-2'-deoxyuridine (IUdR) is a thymidine analogue and specifically incorporated into DNA. When it is labelled with 125I or 131I it permits to reinvestigate these findings by measuring the rate of precursor incorporation into DNA and the rate of loss of labelled DNA in the living animal by means of counting the gamma emission from the incorporated iodine isotopes. In this paper, therefore, an attempt is made to analyse the DNA turnover in the whole body of liver tumour-bearing mice.</p><p><strong>Material and methods: </strong>5-iodo-2'-deoxyuridine (IUdR) labelled with 125iodine was used as DNA precursor. It is a thymidine analogue 5% of which is specifically incorporated into the DNA of those proliferating cells which are in the phase of DNA synthesis at the moment of tracer application. Non-incorporated IUdR (about 95% of the injected amount) is rapidly degraded and excreted within 24 hours. The tracer remains bound to the cellular DNA druing the life span of the labelled cells. After cell death only about 5% of IUdR from DNA breakdown is reutilized. 125I has a half live of 60 days and therefore allows, over periods of weeks, external measurements of the DNA turnover in the living animal without disturbing the physiological environment. The measured loss of DNA-bound 125I reflects almost exclusively the turnover of the labelled cells. Female albino NMRI mice, 2 months old, bearing sarcoma-180 implanted into the right hind leg were intraveneously injected with 2 muCi 125I-UdR. At the time of injection, the tumour had reached in one group of mice an average volume of about 25 mm3 and in another group an average volume of nearly 850 mm3. When implanted into subcutaneous tissue sarcoma-180 rarely produces metastases in parenchymal organs, never in the spleen and--within the first 30 days after implantation--only in ca. 10% of the animals a small metastasis in a single lymphnode (Deodhar and Crile, 1969; Franchi et al., 1968). Whole body measurements were carried out immediately after tracer injection and then daily during the first week and every second or third day in the following 2 weeks in a NaI well counter with a single channel pulse height analyser. The tumour activity was also determined in vivo by a special counting device.</p><p><strong>Results: </strong>In the normal mouse 4 to 6% of injected 125I-UdR is retained in the whole body 24 hours after tracer injection. During the following five days the 125I activity rapidly declines to 0.8% of that of day O immediately after injection. Thereafter the rate of loss of activity greatly diminishes (Fig. I). The first component of the turnover curve reflects an average daily ce","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"159 4","pages":"398-412"},"PeriodicalIF":0.0,"publicationDate":"1976-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12202120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1976-12-01DOI: 10.1016/S0005-8165(76)80169-2
M. Laitio
The material consisted of two intestinal, one gastric body-type and 14 antral-type cases of mucosal metaplasia in the gallbladder.
Outside the mucosal areas mentioned, the epithelium of the gallbladder had undergone great changes. It usually consisted of goblet cells, enterochromaffin cells, superficial gastric-type epithelial islands and antral-type glands. Extensive areas contained non-sulphated acid mucin and neutral mucin. The areas of normal gallbladder epithelium were small.
Das Material bestand aus 17 Fällen von Schleimhautmetaplasien in der Gallenblase. In zwei Fällen war die Schleimhaut intestinalartig umgewandelt, in einem Fall wurden magenkörperartige Veränderungen beobachtet und 14 Fälle wiesen einen antralartigen Umbau der Schleimhaut auf.
Über die Änderungen in der Gallenblasenschleimhaut hinaus wurden auch im Gallenblasenepithel erhebliche Veränderungen beobachtet. Es handelte sich meistens um Becherzellen, enterochromaffine Zellen, Epithelinseln vom Typ des Oberflächenepithels im Magenkörper sowie Drüsen, die denen des Antralbereiches glichen. In umfangreichen Teilen der Gallenblase konnten nonsulphiertes saures Muzin sowie neutrales Muzin nachgewiesen werden. Intaktes Gallenblasenepithel war nur noch wenig erhalten.
{"title":"Intestinal, Gastric Body- and Antral-Type Mucosal Metaplasia in the Gallbladder","authors":"M. Laitio","doi":"10.1016/S0005-8165(76)80169-2","DOIUrl":"10.1016/S0005-8165(76)80169-2","url":null,"abstract":"<div><p>The material consisted of two intestinal, one gastric body-type and 14 antral-type cases of mucosal metaplasia in the gallbladder.</p><p>Outside the mucosal areas mentioned, the epithelium of the gallbladder had undergone great changes. It usually consisted of goblet cells, enterochromaffin cells, superficial gastric-type epithelial islands and antral-type glands. Extensive areas contained non-sulphated acid mucin and neutral mucin. The areas of normal gallbladder epithelium were small.</p></div><div><p>Das Material bestand aus 17 Fällen von Schleimhautmetaplasien in der Gallenblase. In zwei Fällen war die Schleimhaut intestinalartig umgewandelt, in einem Fall wurden magenkörperartige Veränderungen beobachtet und 14 Fälle wiesen einen antralartigen Umbau der Schleimhaut auf.</p><p>Über die Änderungen in der Gallenblasenschleimhaut hinaus wurden auch im Gallenblasenepithel erhebliche Veränderungen beobachtet. Es handelte sich meistens um Becherzellen, enterochromaffine Zellen, Epithelinseln vom Typ des Oberflächenepithels im Magenkörper sowie Drüsen, die denen des Antralbereiches glichen. In umfangreichen Teilen der Gallenblase konnten nonsulphiertes saures Muzin sowie neutrales Muzin nachgewiesen werden. Intaktes Gallenblasenepithel war nur noch wenig erhalten.</p></div>","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"159 3","pages":"Pages 271-279"},"PeriodicalIF":0.0,"publicationDate":"1976-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-8165(76)80169-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11357577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1976-12-01DOI: 10.1016/S0005-8165(76)80170-9
M. Ravid , R. Silman-Socher, Y. Ben Shaul, E. Sohar
A systematic microscopic examination of all elements of the capillary wall was performed on quadriceps muscle biopsies from 9 diabetic patients and 8 controls.
The capillary basement membrane (CBM) was markedly thicker in diabetics; it consisted of several lamellae and contained large vacuoles which were never observed in non-diabetic subjects. Large magnifications revealed fibrils in greater number and markedly larger in diameter in diabetics, these accounting for a considerable volume of the CBM and the adventitia and increased diameter and thickness of the capillary wall, without encroaching on the lumen. The intracellular fibrils in pericytes and endothelial cells were also larger and thicker in diabetic subjects.
The prevalence of fibrillar material in the vascular disease of diabetes mellitus suggests the importance of research into possible measures to arrest fibril formation.
Quadriceps-Muskel-Biopsien von 9 diabetischen und 8 nicht-diabetischen Individuen wurden elektronenmikroskopisch untersucht.
Die Basalmembran der Kapillaren bei Diabetikern war deutlich dicker und bestand aus einigen Lamellen. Sie enthielt große Vakuolen, welche bei den Nicht-Diabetikern fehlten.
Bei starker Vergrößerung wurden in der Basalmembran bei den Diabetikern zahlreiche dicke Fibrillen gefunden. Diese Fibrillen nahmen einen beträchtlichen Teil des Volumens der kapillaren Basalmembran und der Adventitia ein. Der Durchmesser und die Dicke der Kapillarenwand war vergrößert, ohne jedoch das Kapillarlumen einzuengen.
Die intrazellulären Fibrillen in den Perizyten und Endothelzellen der Diabetiker waren ebenfalls dicker.
对9例糖尿病患者和8例对照者的股四头肌活检进行了系统的毛细血管壁所有元素的显微检查。糖尿病患者毛细血管基底膜(CBM)明显增厚;它由几个片层组成，并含有在非糖尿病受试者中从未观察到的大液泡。大镜显示糖尿病患者的原纤维数量更多，直径明显变大，这些原纤维占CBM和外膜的相当大的体积，毛细血管壁的直径和厚度增加，但没有侵犯管腔。糖尿病患者的周细胞和内皮细胞的细胞内原纤维也更大、更厚。糖尿病血管疾病中纤维物质的普遍存在表明研究阻止纤维形成的可能措施的重要性。股四头肌-肌肉-生物扫描9例糖尿病患者和8例夜间糖尿病患者。与糖尿病相关的玄武岩膜研究。他说:“我的孩子，我的孩子，我的孩子，我的孩子。”[1] [0] [0] [0] [0] [0] [0] [0] [0] [0][Diese fibren nahmen einen beträchtlichen]对毛细管玄武岩膜和外膜体积的研究。Der Durchmesser and die Dicke Der Kapillarenwand war vergrößert, ohne jedoch das Kapillarlumen einzuengen。死亡intrazellulären糖尿病患者外周血纤维蛋白与内皮素的关系。
{"title":"Quantitative Electron Microscopic Study of Capillaries in Diabetes mellitus","authors":"M. Ravid , R. Silman-Socher, Y. Ben Shaul, E. Sohar","doi":"10.1016/S0005-8165(76)80170-9","DOIUrl":"10.1016/S0005-8165(76)80170-9","url":null,"abstract":"<div><p>A systematic microscopic examination of all elements of the capillary wall was performed on quadriceps muscle biopsies from 9 diabetic patients and 8 controls.</p><p>The capillary basement membrane (CBM) was markedly thicker in diabetics; it consisted of several lamellae and contained large vacuoles which were never observed in non-diabetic subjects. Large magnifications revealed fibrils in greater number and markedly larger in diameter in diabetics, these accounting for a considerable volume of the CBM and the adventitia and increased diameter and thickness of the capillary wall, without encroaching on the lumen. The intracellular fibrils in pericytes and endothelial cells were also larger and thicker in diabetic subjects.</p><p>The prevalence of fibrillar material in the vascular disease of diabetes mellitus suggests the importance of research into possible measures to arrest fibril formation.</p></div><div><p>Quadriceps-Muskel-Biopsien von 9 diabetischen und 8 nicht-diabetischen Individuen wurden elektronenmikroskopisch untersucht.</p><p>Die Basalmembran der Kapillaren bei Diabetikern war deutlich dicker und bestand aus einigen Lamellen. Sie enthielt große Vakuolen, welche bei den Nicht-Diabetikern fehlten.</p><p>Bei starker Vergrößerung wurden in der Basalmembran bei den Diabetikern zahlreiche dicke Fibrillen gefunden. Diese Fibrillen nahmen einen beträchtlichen Teil des Volumens der kapillaren Basalmembran und der Adventitia ein. Der Durchmesser und die Dicke der Kapillarenwand war vergrößert, ohne jedoch das Kapillarlumen einzuengen.</p><p>Die intrazellulären Fibrillen in den Perizyten und Endothelzellen der Diabetiker waren ebenfalls dicker.</p></div>","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"159 3","pages":"Pages 280-291"},"PeriodicalIF":0.0,"publicationDate":"1976-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-8165(76)80170-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12195679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Postmortem investigation of esophageal varices and the portal system is difficult, because veins are collapsed at autopsy. The demonstration of varices is possible by illumination of the isolated mucous membrane or by puncturing esophageal varices and filing them with different materials.
Material and methods: We use a 15% aqueous gelatine solution (if needed with additional barium sulfate for x-ray examination) which is supplemented by 40% formaldehyde (40 ml in 1 1 gelatine solution). The superior mesenteric vein is catheterized and filled by a clyster-pump.
Results: The mixture of gelatine and formaldehyde hardens within a few minutes. The autopsy is delayed only about half an hour. Within this time the portal system is well outlined. This method can show exactly the drainage of the portal system into the inferior vena cava. In cases of portocaval shunt or of esophageal transsection the result of the operation can be verified. The localization of the bleeding source of esophageal varices can be demonstrated by escape of the filling mass.
{"title":"[Postmortem demonstration of portal vein collateral circulation (author's transl)].","authors":"G Brandt, M Stolte","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Introduction: </strong>Postmortem investigation of esophageal varices and the portal system is difficult, because veins are collapsed at autopsy. The demonstration of varices is possible by illumination of the isolated mucous membrane or by puncturing esophageal varices and filing them with different materials.</p><p><strong>Material and methods: </strong>We use a 15% aqueous gelatine solution (if needed with additional barium sulfate for x-ray examination) which is supplemented by 40% formaldehyde (40 ml in 1 1 gelatine solution). The superior mesenteric vein is catheterized and filled by a clyster-pump.</p><p><strong>Results: </strong>The mixture of gelatine and formaldehyde hardens within a few minutes. The autopsy is delayed only about half an hour. Within this time the portal system is well outlined. This method can show exactly the drainage of the portal system into the inferior vena cava. In cases of portocaval shunt or of esophageal transsection the result of the operation can be verified. The localization of the bleeding source of esophageal varices can be demonstrated by escape of the filling mass.</p>","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"159 3","pages":"307-13"},"PeriodicalIF":0.0,"publicationDate":"1976-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12195683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1976-12-01DOI: 10.1016/S0005-8165(76)80167-9
E.D. Wachsmuth , J.P. Stoye
Sections of hypernephroid carcinoma from 20 cases were investigated for aldolase isozymes A and B by a mixed aggregation immuno-cytochemical technique, and for the brush border membrane enzymes aminopeptidase and alkaline phosphatase by conventional histochemical techniques. It was found that the cases could be grouped into four types: type 1 (1 case) contained all 4 enzymes; type 2 (7 cases) contained all enzymes except aldolase-B; type 3 (7 cases) possessed aldolase-A and one brush border membrane enzyme; type 4 (5 cases) contained only aldolase-A. The aldolase-A concentration in all tumor cells was higher than that in proximal tubule cells, whereas the concentration of the two brush border enzymes was lower. In cases type 2 and 3, but not in type 4 occasional patches of cells were found to contain some aldolase-B and/or higher amounts of the brush border enzymes than the surrounding cells. No correlation was observed between clear cell and granular cell hypernephroid carcinomas or the invasiveness or the nuclear polymorphism of the tumors on the one hand with their enzyme type on the other.
These histological enzyme analyses suggest that most, if not all, hypernephroid carcinomas are derived from kidney proximal tubule cells and that the tumor cells then progressively lose aldolase-B, and subsequently the brush border enzymes, but at the same time producing more aldolase-A. The presence of the enzyme-rich patches suggest different patterns of proliferation and differentiation among the tumor cell population.
Three tumors other than hypernephroid carcinoma were also examined in this way. The results suggest that histoenzymological analyses are of general applicability in studies of tumor progression. They should also be useful for biopsy and aspiration cytology.
Hypernephroide Karzinome von 20 Patienten wurden nach operativer Exstirpation auf ihren Gehalt an Aldolase-A, Aldolase-B und den Bürstensaumenzymen, Aminopeptidase und alkalische Phosphatase, in Gewebehomogenaten und am Gewebeschnitt untersucht. Eine immunozytochemische Methode, beruhend auf dem immunochemischen Prinzip einer gemischten Aggregation, diente dem Nachweis und der Lokalisation der Aldolasen, übliche histochemische Methoden dem Nachweis der beiden Bürstensaummembranenzyme Aminopeptidase und alkalische Phosphatase. Die Ergebnisse von Homogenatanalysen waren für eine Klassifizierung der Progredienz des Karzinoms nicht brauchbar im Gegensatz zur Gewebsschnittanalyse. Letztere erlaubte eine Einteilung der Karzinome in 4 Typen: Typ 1 (1 Fall) besaß alle 4 Enzyme, Typ 2 (7 Fälle) enthielt alle Enzyme außer Aldolase-B, Typ 3 (7 Fälle) besaß Aldolase-A und eines der Bürstensaummembranenzyme, Typ 4 (5 Fälle) enthielt nur Aldolase-A, keines der anderen 3 Enzyme war nachweisbar. Die Konzentrationen an Aldolase-A waren in allen Tumorzellen höher, die der Bürstensaummembranenzyme und der Aldolase-B niedriger als in den Hauptstücken des Nephrons. In de
采用混合聚集免疫-细胞化学技术检测20例甲状腺癌组织中醛缩酶同工酶A和B的表达，并用常规组织化学技术检测刷状缘膜酶氨基肽酶和碱性磷酸酶的表达。结果发现，病例可分为4种类型:1型(1例)4种酶均含有;2型(7例)除醛缩酶b外，其余酶均含有;3型(7例)含有醛缩酶- a和1个刷状膜酶;4型(5例)仅含有醛缩酶a。所有肿瘤细胞的醛缩酶- a浓度均高于近端小管细胞，而两种刷边酶的浓度较低。在2型和3型病例中，偶尔发现细胞斑块含有一些醛缩酶- b和/或比周围细胞含量更高的刷状边缘酶，但在4型病例中没有。透明细胞型和颗粒细胞型肾样肥大癌，肿瘤的侵袭性和细胞核多态性与其酶类型没有相关性。这些组织学酶分析表明，大多数(如果不是全部的话)肾性高肾癌来源于肾近端小管细胞，肿瘤细胞随后逐渐失去醛缩酶- b，随后失去刷状边界酶，但同时产生更多的醛缩酶- a。富酶斑块的存在表明肿瘤细胞群中存在不同的增殖和分化模式。除肾高性癌外，另外三种肿瘤也用这种方法进行了检查。结果表明，组织酶学分析在肿瘤进展研究中具有普遍适用性。它们也可用于活检和细胞学抽吸。高肾素Karzinome(20例患者肾小球手术)的提取:在胃匀浆和胃匀浆中提取:Aldolase-A、Aldolase-B、den bstensaumase、氨基肽酶和碱性磷酸酶。Eine免疫酶化法，beruhend免疫酶化法，beruhend免疫酶化法，diente dem Nachweis和der lokalization der Aldolasen, bliche组织化学方法dem Nachweis和der beiden bstensaum膜酶-氨基肽酶和碱性磷酸酶。同质性分析的研究进展[j]， [j]， [j]， [j]。Letztere erlaubte eine Einteilung der Karzinome in 4 Typen: type1 (1 Fall) besasß alle 4酶，type2 (7 Fälle) enthielt alle酶außer Aldolase-B, type3 (7 Fälle) besasß Aldolase-A和eines der bstenssaum膜酶，type4 (5 Fälle) enthielt nur Aldolase-A, keines der anderen3酶war nachweisbar。肿瘤组织中Aldolase-A和Konzentrationen与Aldolase-A的关系höher, hauptstencen与Nephrons组织中Aldolase-B的关系。In den Typen 2和3,aber nicht在Typen 4, kamen gelegentlich Zellhaufen vor, die geringe Mengen和Aldolase-B und/oder höhere Konzentrationen和brstensaum酶besitzen也die sie umgebenden brigen Tumorzellen。Keine相关性:best zwischen diesen酶促性与肾形态的关系;best zwischen diesen酶促性与肾形态的关系;best zwischen diesen酶促性与肾形态的关系;4酶在肿瘤细胞内的定位与降解Fälle kann angenommen werden，高肾细胞内的Karzinome von von hauptsten，肾细胞内的Nephrons abstammen和肿瘤细胞内的Aldolase-B和dann - stenssaum膜酶verlieren, aber bereits von Anfang和mehr Aldolase-A prodezien。《肿瘤生物学》erwähnten Zellhaufen läßt recht terschiedliche prolifationen and Differenzierungen in einem scheinbar einheitlichen tumor band vermuten。Ähnliche Ergebnisse wurden and einer Anzahl anderdertumorengewomnen。Somit erscheint es möglich, durch酶-和insbesonere durch同工酶-在zellenf细胞活组织检查，der die抽吸，zytologie差异分级和Homogenität eines肿瘤的研究。
{"title":"A Classification of Tumor Development Based on an Analysis of Enzymes in Tissue Sections of Hypernephroid Carcinoma in Man","authors":"E.D. Wachsmuth , J.P. Stoye","doi":"10.1016/S0005-8165(76)80167-9","DOIUrl":"10.1016/S0005-8165(76)80167-9","url":null,"abstract":"<div><p>Sections of hypernephroid carcinoma from 20 cases were investigated for aldolase isozymes A and B by a mixed aggregation immuno-cytochemical technique, and for the brush border membrane enzymes aminopeptidase and alkaline phosphatase by conventional histochemical techniques. It was found that the cases could be grouped into four types: type 1 (1 case) contained all 4 enzymes; type 2 (7 cases) contained all enzymes except aldolase-B; type 3 (7 cases) possessed aldolase-A and one brush border membrane enzyme; type 4 (5 cases) contained only aldolase-A. The aldolase-A concentration in all tumor cells was higher than that in proximal tubule cells, whereas the concentration of the two brush border enzymes was lower. In cases type 2 and 3, but not in type 4 occasional patches of cells were found to contain some aldolase-B and/or higher amounts of the brush border enzymes than the surrounding cells. No correlation was observed between clear cell and granular cell hypernephroid carcinomas or the invasiveness or the nuclear polymorphism of the tumors on the one hand with their enzyme type on the other.</p><p>These histological enzyme analyses suggest that most, if not all, hypernephroid carcinomas are derived from kidney proximal tubule cells and that the tumor cells then progressively lose aldolase-B, and subsequently the brush border enzymes, but at the same time producing more aldolase-A. The presence of the enzyme-rich patches suggest different patterns of proliferation and differentiation among the tumor cell population.</p><p>Three tumors other than hypernephroid carcinoma were also examined in this way. The results suggest that histoenzymological analyses are of general applicability in studies of tumor progression. They should also be useful for biopsy and aspiration cytology.</p></div><div><p>Hypernephroide Karzinome von 20 Patienten wurden nach operativer Exstirpation auf ihren Gehalt an Aldolase-A, Aldolase-B und den Bürstensaumenzymen, Aminopeptidase und alkalische Phosphatase, in Gewebehomogenaten und am Gewebeschnitt untersucht. Eine immunozytochemische Methode, beruhend auf dem immunochemischen Prinzip einer gemischten Aggregation, diente dem Nachweis und der Lokalisation der Aldolasen, übliche histochemische Methoden dem Nachweis der beiden Bürstensaummembranenzyme Aminopeptidase und alkalische Phosphatase. Die Ergebnisse von Homogenatanalysen waren für eine Klassifizierung der Progredienz des Karzinoms nicht brauchbar im Gegensatz zur Gewebsschnittanalyse. Letztere erlaubte eine Einteilung der Karzinome in 4 Typen: Typ 1 (1 Fall) besaß alle 4 Enzyme, Typ 2 (7 Fälle) enthielt alle Enzyme außer Aldolase-B, Typ 3 (7 Fälle) besaß Aldolase-A und eines der Bürstensaummembranenzyme, Typ 4 (5 Fälle) enthielt nur Aldolase-A, keines der anderen 3 Enzyme war nachweisbar. Die Konzentrationen an Aldolase-A waren in allen Tumorzellen höher, die der Bürstensaummembranenzyme und der Aldolase-B niedriger als in den Hauptstücken des Nephrons. In de","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"159 3","pages":"Pages 229-248"},"PeriodicalIF":0.0,"publicationDate":"1976-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-8165(76)80167-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12197098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1976-12-01DOI: 10.1016/S0005-8165(76)80030-3
H. Buerki , B. Massner , H. Cottier , A.D. Chanana , D.D. Joel , E.P. Cronkite
Thoracic duct-rumen fistulae (TDRF) and low or high doses of Prednisolone were employed to induce depletion of blood and lymph node lymphocytes in calves. Elimination of the easily mobilizable pool of lymphocytes was almost complete within 6 days after commencing TDRF. Blood lymphocyte counts were reduced to approximately ⅓ of pre-TDRF levels. Prednisolone in low doses did not decrease the number of blood lymphocytes. High doses of Prednisolone induced a moderate, progressive blood lymphocytopenia. Both TDRF and low and high dose Prednisolone schedules induced a significant reduction of lymphocytes in lymph nodes. The loss of small lymphocytes from lymph nodes was almost entirely restricted to the diffuse cortical (paracortical) zone. The lymphocyte counts in the follicular cortex and in the medulla were not significantly affected. Almost complete disappearance of germinal centers was observed in lymph nodes of calves subjected to TDRF or high doses of Prednisolone.
Im Zuge vergleichender Untersuchungen über experimentelle Lymphopenie wurden Kälbern entweder eine Fistel zwischen Ductus thoracicus und Magen (TDRF, thoracic duct-rumen fistula) angelegt oder Prednisolon in niedriger und hoher Dosierung verabreicht. Innerhalb der ersten 6 Tage nach Herstellung der TDRF war fast der ganze leicht mobilisierbare Teil der Lymphozyten ausgeschaltet, und die Blutlymphozytenwerte fielen auf ungefähr ein Drittel der Ausgangswerte. Prednisolon in niedriger Dosierung beeinflußte die Zahl der Blutlymphozyten kaum, bewirkte indessen in hoher Dosis eine mäßige progressive Lymphozytopenie. In den Lymphknoten wurde die Gesamtzahl der Lymphozyten sowohl durch TDRF als auch durch niedrige und hohe Dosen von Prednisolon signifikant vermindert. Dieser Lymphozytenverlust in den Lymphknoten beschränkte sich fast ausschließlich auf die tiefe kortikale (parakortikale) Zone, die überwiegend T-Zellen enthält. Die von mehr oder weniger seßhaften B-Lymphozyten besiedelten Primärfollikel, die lymphozytäre Mantelzone der Sekundärfollikel und die Medulla blieben fast unverändert, während die Keimzentren durch Anlegen einer TDRF oder durch hohe Dosen von Prednisolon weitgehend zum Verschwinden gebracht wurden.
采用胸椎管-瘤胃瘘管(TDRF)和低剂量或高剂量强的松龙诱导犊牛血液和淋巴结淋巴细胞耗竭。在开始TDRF后的6天内，易于调动的淋巴细胞池几乎完全消除。血液淋巴细胞计数减少到tdrf前水平的约1 / 3。低剂量强的松龙不降低血液淋巴细胞的数量。大剂量强的松龙引起中度进行性血淋巴细胞减少。TDRF和低剂量和高剂量强的松龙方案均诱导淋巴结淋巴细胞显著减少。淋巴结小淋巴细胞的丢失几乎完全局限于弥漫性皮质(皮质旁)区。滤泡皮层和髓质的淋巴细胞计数无明显变化。在接受TDRF或高剂量强的松龙治疗的小牛淋巴结中，观察到生发中心几乎完全消失。在此基础上，建立了胸管-胃瘘(TDRF，胸管-胃瘘)角与强的松松在腹腔和腹腔内的应用。内halb der ersten 6 age nach Herstellung der TDRF war fast der ganze leicht mobilisierbare Teil der Lymphozyten ausgeschaltet, and die Blutlymphozytenwerte fielen auf ungefähr ein Drittel der Ausgangswerte。强的松龙对老年痴呆患者的影响与老年痴呆患者的影响与老年痴呆患者的影响与老年痴呆患者的影响与老年痴呆患者的影响与老年痴呆患者的影响与老年痴呆患者的影响与老年痴呆患者的影响与老年痴呆患者的影响之间的关系。In den lymphoknoten wurde die Gesamtzahl der Lymphozyten sowhh . TDRF是一种具有显著性的恶性肿瘤。dieer Lymphozytenverlust in den lymphoknoten beschränkte sich fast ausschließlich auf die tiefe kortikale (parakortikale) Zone, die berwiegend T-Zellen enthält。Die von mehr oder weniger ß ßhaften B-Lymphozyten beiedelten Primärfollikel, Die lymphozytäre Mantelzone der Sekundärfollikel and Die Medulla blieben fast unverändert, während Die Keimzentren durch Anlegen einer TDRF der durch hohe Dosen von Prednisolon weitgehend zum Verschwinden gebracht wurden。
{"title":"Effects of Lymph Diversion Versus Corticosteroids on Blood and Lymphocyte Pools","authors":"H. Buerki , B. Massner , H. Cottier , A.D. Chanana , D.D. Joel , E.P. Cronkite","doi":"10.1016/S0005-8165(76)80030-3","DOIUrl":"10.1016/S0005-8165(76)80030-3","url":null,"abstract":"<div><p>Thoracic duct-rumen fistulae (TDRF) and low or high doses of Prednisolone were employed to induce depletion of blood and lymph node lymphocytes in calves. Elimination of the easily mobilizable pool of lymphocytes was almost complete within 6 days after commencing TDRF. Blood lymphocyte counts were reduced to approximately ⅓ of pre-TDRF levels. Prednisolone in low doses did not decrease the number of blood lymphocytes. High doses of Prednisolone induced a moderate, progressive blood lymphocytopenia. Both TDRF and low and high dose Prednisolone schedules induced a significant reduction of lymphocytes in lymph nodes. The loss of small lymphocytes from lymph nodes was almost entirely restricted to the diffuse cortical (paracortical) zone. The lymphocyte counts in the follicular cortex and in the medulla were not significantly affected. Almost complete disappearance of germinal centers was observed in lymph nodes of calves subjected to TDRF or high doses of Prednisolone.</p></div><div><p>Im Zuge vergleichender Untersuchungen über experimentelle Lymphopenie wurden Kälbern entweder eine Fistel zwischen Ductus thoracicus und Magen (TDRF, thoracic duct-rumen fistula) angelegt oder Prednisolon in niedriger und hoher Dosierung verabreicht. Innerhalb der ersten 6 Tage nach Herstellung der TDRF war fast der ganze leicht mobilisierbare Teil der Lymphozyten ausgeschaltet, und die Blutlymphozytenwerte fielen auf ungefähr ein Drittel der Ausgangswerte. Prednisolon in niedriger Dosierung beeinflußte die Zahl der Blutlymphozyten kaum, bewirkte indessen in hoher Dosis eine mäßige progressive Lymphozytopenie. In den Lymphknoten wurde die Gesamtzahl der Lymphozyten sowohl durch TDRF als auch durch niedrige und hohe Dosen von Prednisolon signifikant vermindert. Dieser Lymphozytenverlust in den Lymphknoten beschränkte sich fast ausschließlich auf die tiefe kortikale (parakortikale) Zone, die überwiegend T-Zellen enthält. Die von mehr oder weniger seßhaften B-Lymphozyten besiedelten Primärfollikel, die lymphozytäre Mantelzone der Sekundärfollikel und die Medulla blieben fast unverändert, während die Keimzentren durch Anlegen einer TDRF oder durch hohe Dosen von Prednisolon weitgehend zum Verschwinden gebracht wurden.</p></div>","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"159 4","pages":"Pages 343-350"},"PeriodicalIF":0.0,"publicationDate":"1976-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-8165(76)80030-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12202119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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