Edegbo Emmanuel, Oseni Okolo Martin-Luther, Sunday Adegoke Adetunji, Arome Omatola Cornelius, Mudiwa Benjamin, Oyiguh Abraham Joseph, Eneojo Akor Monday, Adeiza Zakari David, Zainab A. Adebayo, Omale Sunday, Y. Abdulrazaq, Musa Qasim, M. Danjuma
.
{"title":"Phytochemical screening and antifungal activity of Cassia alata (Linn.) crude leaf extracts","authors":"Edegbo Emmanuel, Oseni Okolo Martin-Luther, Sunday Adegoke Adetunji, Arome Omatola Cornelius, Mudiwa Benjamin, Oyiguh Abraham Joseph, Eneojo Akor Monday, Adeiza Zakari David, Zainab A. Adebayo, Omale Sunday, Y. Abdulrazaq, Musa Qasim, M. Danjuma","doi":"10.5897/ajmr2023.9711","DOIUrl":"https://doi.org/10.5897/ajmr2023.9711","url":null,"abstract":".","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49447198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alejandra Istúriz-Zapata María, Bautista-Baños Silvia, Luis Acosta-Rodríguez José, Hernández-López Mónica, Leticia Barrera-Necha Laura
{"title":"Morphological, pathogenic and molecular characterization of fungal species associated with mango fruits in Mexico","authors":"Alejandra Istúriz-Zapata María, Bautista-Baños Silvia, Luis Acosta-Rodríguez José, Hernández-López Mónica, Leticia Barrera-Necha Laura","doi":"10.5897/ajmr2023.9707","DOIUrl":"https://doi.org/10.5897/ajmr2023.9707","url":null,"abstract":"","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49211467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Stephan Nascente Adriano, Zainab Temitope Ishola, C. Marta, Ricardo Cabral Cruz Dennis
Multifunctional microorganisms can significantly affect root and shoot development of upland rice seedling that could provide an increase in the crop grain yield. The objective of this study was to determine the effect of single and combined microorganism on the root and shoot development of upland rice seedlings. The experiment was arranged in a completely randomized design with treatments consisting of the upland rice seeds treated with single and combined multifunctional microorganisms (M01 ( Serratia marcescens ) , M02 ( Bacillus toyonensis ), M03 ( Phanerochaete australis) , M04 ( Trichoderma koningiopsis ), M05 ( Azospirillum brasilense ), M06 ( Azospirillum species), M07 ( Bacillus species), M08 to M28 (combination of these microorganisms) and M29 (control – no microorganism). S. marcescens with B. toyonensis led to the greatest increase (296%) in root length relative to the control. B. toyonensis with A. brasilense greatly increased root surface area by 209% in comparison to the control. An increased root diameter by 36% was recorded for upland rice inoculated with A. brasilense with Bacillus spp. in relation to the control. P. australis with Bacillus spp. greatly increased root volume (47%) in comparison to the control. It can be concluded that multifunctional microorganisms enhanced root length, root surface area, root diameter and volume, and provided better root development
{"title":"Beneficial microorganisms as affecting root development of upland rice","authors":"Stephan Nascente Adriano, Zainab Temitope Ishola, C. Marta, Ricardo Cabral Cruz Dennis","doi":"10.5897/ajmr2023.9695","DOIUrl":"https://doi.org/10.5897/ajmr2023.9695","url":null,"abstract":"Multifunctional microorganisms can significantly affect root and shoot development of upland rice seedling that could provide an increase in the crop grain yield. The objective of this study was to determine the effect of single and combined microorganism on the root and shoot development of upland rice seedlings. The experiment was arranged in a completely randomized design with treatments consisting of the upland rice seeds treated with single and combined multifunctional microorganisms (M01 ( Serratia marcescens ) , M02 ( Bacillus toyonensis ), M03 ( Phanerochaete australis) , M04 ( Trichoderma koningiopsis ), M05 ( Azospirillum brasilense ), M06 ( Azospirillum species), M07 ( Bacillus species), M08 to M28 (combination of these microorganisms) and M29 (control – no microorganism). S. marcescens with B. toyonensis led to the greatest increase (296%) in root length relative to the control. B. toyonensis with A. brasilense greatly increased root surface area by 209% in comparison to the control. An increased root diameter by 36% was recorded for upland rice inoculated with A. brasilense with Bacillus spp. in relation to the control. P. australis with Bacillus spp. greatly increased root volume (47%) in comparison to the control. It can be concluded that multifunctional microorganisms enhanced root length, root surface area, root diameter and volume, and provided better root development","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41657964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muigai Monicah, Odiwuor Odhiambo Nixon, Kiilu Muli Joshua, Mugweru Julius, M. Romano
Fusarium oxysporum f.sp Lycopersici (Fusarium wilt) is a serious plant pathogen that can cause yield losses >80% in tomatoes. The disease is controlled mainly by use of synthetic pesticides, which may be harmful to both humans and the environment. In this study, the potential use of antagonistic bacteria was reported for the control of fusarium wilt. First, we isolated and characterized fungi from tomato plants with wilt symptoms. Analysis of the ITS gene indicated fungi from the genus Fusarium, Alternaria, Plectosphaerella , Aspergillus , Gibellulopsis , Trichoderma , Papiliotrema , Rhodotorula , Mucor , Ustilago , Sporothrix and Cumuliphoma . 20 bacterial isolates were then screened from soils collected around the tomato plants for antagonistic activity against F.oxysporum using soft agar overlay method. Bacterial isolates showing inhibition zone diameters >3mm and consistent in reducing mycelial growth of the pathogen were further characterized and subsequently used for field experiments which were conducted during the short rain season in 2021 and 2022. The isolates grew at an optimum salt concentration of 5 - 10 % (w/v), pH range of 5 - 8 and a temperature of 30°C. Phylogenetically, they were affiliated to Bacillus subtilis , Paenibacillus polymyxa , Brevibacillus laterosporus , Bacillus velezensis , Bacillus amyloliquefaciens and Paenibacillus poriae whereas one of the isolates was identified as Myroides odoratimimus . Results from the field experiments showed that all selected strains significantly reduced average disease incidence (mean ± SE) for both years on day 14 (14.4% ± 3.49), day 28 (15.9% ± 3.44) and day 42 (15.2% ± 2.825) respectively. Therefore, these isolates are good candidates for the development of effective biological control programs against F. oxysporum f.sp Lycopersici affecting tomatoes.
{"title":"Use of bacterial biocontrol agents for the control of Fusarium oxysporum f.sp. Lycopersici (Fusarium wilt) in tomatoes","authors":"Muigai Monicah, Odiwuor Odhiambo Nixon, Kiilu Muli Joshua, Mugweru Julius, M. Romano","doi":"10.5897/ajmr2023.9700","DOIUrl":"https://doi.org/10.5897/ajmr2023.9700","url":null,"abstract":"Fusarium oxysporum f.sp Lycopersici (Fusarium wilt) is a serious plant pathogen that can cause yield losses >80% in tomatoes. The disease is controlled mainly by use of synthetic pesticides, which may be harmful to both humans and the environment. In this study, the potential use of antagonistic bacteria was reported for the control of fusarium wilt. First, we isolated and characterized fungi from tomato plants with wilt symptoms. Analysis of the ITS gene indicated fungi from the genus Fusarium, Alternaria, Plectosphaerella , Aspergillus , Gibellulopsis , Trichoderma , Papiliotrema , Rhodotorula , Mucor , Ustilago , Sporothrix and Cumuliphoma . 20 bacterial isolates were then screened from soils collected around the tomato plants for antagonistic activity against F.oxysporum using soft agar overlay method. Bacterial isolates showing inhibition zone diameters >3mm and consistent in reducing mycelial growth of the pathogen were further characterized and subsequently used for field experiments which were conducted during the short rain season in 2021 and 2022. The isolates grew at an optimum salt concentration of 5 - 10 % (w/v), pH range of 5 - 8 and a temperature of 30°C. Phylogenetically, they were affiliated to Bacillus subtilis , Paenibacillus polymyxa , Brevibacillus laterosporus , Bacillus velezensis , Bacillus amyloliquefaciens and Paenibacillus poriae whereas one of the isolates was identified as Myroides odoratimimus . Results from the field experiments showed that all selected strains significantly reduced average disease incidence (mean ± SE) for both years on day 14 (14.4% ± 3.49), day 28 (15.9% ± 3.44) and day 42 (15.2% ± 2.825) respectively. Therefore, these isolates are good candidates for the development of effective biological control programs against F. oxysporum f.sp Lycopersici affecting tomatoes.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49244127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. M. Ginethon, S. Abdul, W. M. Apia, H. Rhodes, S. Robert, V. G. K. Erick, L. Ladslaus
{"title":"Prevalence of Bartonella spp. in rodent and shrew species trapped in Kigoma and Morogoro Regions, Tanzania: A public health concern","authors":"G. M. Ginethon, S. Abdul, W. M. Apia, H. Rhodes, S. Robert, V. G. K. Erick, L. Ladslaus","doi":"10.5897/ajmr2023.9697","DOIUrl":"https://doi.org/10.5897/ajmr2023.9697","url":null,"abstract":"","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43713947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cross-site comparison of coastal bacterial occurrences and diversity at selected sites along the North Atlantic Ocean in Monrovia, Liberia","authors":"A. O. Ola","doi":"10.5897/ajmr2023.9690","DOIUrl":"https://doi.org/10.5897/ajmr2023.9690","url":null,"abstract":"","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49612723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The use of essentials oils (EOs) as alternative of nitrites and nitrates in sausages will be proposed in this study. For this purpose, the minimal inhibitory concentration (MIC) of white Piper nigrum of Penja , Thymus vulgaris and Syzigiuim aromaticum EOs were determined in vitro against Escherichia coli ATCC25922, Salmonella enteritidis 155A and Staphylococcus aureus NCTC10652. It follows from this study that, T. vulgaris EO was active on E. coli ATCC25922, S. aureus NCTC10652 and S. enteritidis 155A with MICs of 312.5, 625 and 1250 ppm, respectively. As for S. aromaticum EO, it reduced the same germs with MICs of 1250, 2500 and 5000 ppm, respectively. The combination of MICs of 1250 ( T. vulgaris ) and 5000 ppm ( S. aromaticum ) was selected (CMICR) for its bactericidal effect on 03 pathogens. The CMICR totally reduced E. coli ATCC25922 and S. enteritidis 155A after 3 days of storage. The sensory analysis shows that SAUS 324 and SAUS 589 produced with white P. nigrum of Penja were appreciated by the panelists. The combination of CMICR and white P. nigrum of Penja can be considered as an alternative to chemical preservatives to limit the growth of bacteria and improve the sensory characteristics of sausages.
本研究将提出在香肠中使用精油作为亚硝酸盐和硝酸盐的替代品。为此，测定了荷花白胡椒、麝香草和香仙子对大肠杆菌ATCC25922、肠炎沙门氏菌155A和金黄色葡萄球菌NCTC10652的最小抑菌浓度(MIC)。由本研究可知，T. vulgaris EO对大肠杆菌ATCC25922、金黄色葡萄球菌NCTC10652和肠炎沙门氏菌155A的mic值分别为312.5、625和1250 ppm。对于S. aromaticum EO，在mic分别为1250、2500和5000ppm时，其对相同病菌的抑制效果较好。选择1250 (T. vulgaris)和5000 ppm (S. aromaticum)的mic组合(CMICR)对03种病原菌的杀菌效果进行了研究。CMICR在储存3天后完全减少大肠杆菌ATCC25922和肠炎沙门氏菌155A。感官分析表明，以Penja白色黑孢菌为原料生产的saus324和saus589得到了与会嘉宾的好评。CMICR与Penja白色黑孢杆菌的组合可以被认为是化学防腐剂的替代品，以限制细菌的生长，改善香肠的感官特性。
{"title":"Effect of Piper nigrum, Thymus vulgaris and Syzigiuim aromaticum essential oils on the microbiological and sensory quality of Pork sausages during preservation","authors":"R. Tchikoua, Foka Desoeuvres Fogang, Kamdem Sylvain Leroy Sado, Ngang Jean-Justin Essia","doi":"10.5897/ajmr2023.9687","DOIUrl":"https://doi.org/10.5897/ajmr2023.9687","url":null,"abstract":"The use of essentials oils (EOs) as alternative of nitrites and nitrates in sausages will be proposed in this study. For this purpose, the minimal inhibitory concentration (MIC) of white Piper nigrum of Penja , Thymus vulgaris and Syzigiuim aromaticum EOs were determined in vitro against Escherichia coli ATCC25922, Salmonella enteritidis 155A and Staphylococcus aureus NCTC10652. It follows from this study that, T. vulgaris EO was active on E. coli ATCC25922, S. aureus NCTC10652 and S. enteritidis 155A with MICs of 312.5, 625 and 1250 ppm, respectively. As for S. aromaticum EO, it reduced the same germs with MICs of 1250, 2500 and 5000 ppm, respectively. The combination of MICs of 1250 ( T. vulgaris ) and 5000 ppm ( S. aromaticum ) was selected (CMICR) for its bactericidal effect on 03 pathogens. The CMICR totally reduced E. coli ATCC25922 and S. enteritidis 155A after 3 days of storage. The sensory analysis shows that SAUS 324 and SAUS 589 produced with white P. nigrum of Penja were appreciated by the panelists. The combination of CMICR and white P. nigrum of Penja can be considered as an alternative to chemical preservatives to limit the growth of bacteria and improve the sensory characteristics of sausages.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46554657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tora Dkawlma, Hentschel Ute, Lips Stefan, Schmitt-Jansen Mechthild, Borchert Erik
{"title":"16S rRNA gene sequence analysis of the microbial community on microplastic samples from the North Atlantic and Great Pacific Garbage Patches","authors":"Tora Dkawlma, Hentschel Ute, Lips Stefan, Schmitt-Jansen Mechthild, Borchert Erik","doi":"10.5897/ajmr2022.9682","DOIUrl":"https://doi.org/10.5897/ajmr2022.9682","url":null,"abstract":"","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48043512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Prevalence of rotavirus group A and associated genotypes detected in calves in Southern Highlands and Eastern Tanzania","authors":"Jaili Malakalinga Joseph, Misinzo Gerald, Mutani Msalya George, John Shayo Mariana, Reuben Kazwala Rudovick","doi":"10.5897/ajmr2022.9667","DOIUrl":"https://doi.org/10.5897/ajmr2022.9667","url":null,"abstract":"","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48081201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Molecular characterization of heavy metal resistant Proteus species","authors":"Adebo A., U. E., O. i","doi":"10.5897/ajmr2022.9671","DOIUrl":"https://doi.org/10.5897/ajmr2022.9671","url":null,"abstract":"","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48677606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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