F. Guo, Jie Zhou, Chuandong Qi, Jiaping Fu, Jinping Wu
In the springs of 2020 and 2021, with a temperature of 15°C, root rot on garlic were widespread in Enshi, Hubei Province, China. Based on micro-morphological and cultural characteristics, the pathogen was identified as a Fusarium sp. Further, based on multilocus (ITS, EF-1α) phylogenic data, the strains were identified as Fusarium oxysporum. Koch’s postulates were thus fulfilled by pathogenicity tests on garlic seedlings cultured in vitro.
{"title":"First report of Fusarium oxysporum causing root rot of garlic in China","authors":"F. Guo, Jie Zhou, Chuandong Qi, Jiaping Fu, Jinping Wu","doi":"10.5897/ajmr2021.9586","DOIUrl":"https://doi.org/10.5897/ajmr2021.9586","url":null,"abstract":"In the springs of 2020 and 2021, with a temperature of 15°C, root rot on garlic were widespread in Enshi, Hubei Province, China. Based on micro-morphological and cultural characteristics, the pathogen was identified as a Fusarium sp. Further, based on multilocus (ITS, EF-1α) phylogenic data, the strains were identified as Fusarium oxysporum. Koch’s postulates were thus fulfilled by pathogenicity tests on garlic seedlings cultured in vitro.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45456097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Itaype Ibrahim Bashir Isam-Eldeen, Yousri Muatsim Hamid AlaaEldin, A. I. Mohamed, H. A. Eltayib
{"title":"Isolation of potentially pathogenic bacteria from Musca domestica captured in hospitals and slaughterhouses, Khartoum state, Sudan","authors":"Itaype Ibrahim Bashir Isam-Eldeen, Yousri Muatsim Hamid AlaaEldin, A. I. Mohamed, H. A. Eltayib","doi":"10.5897/ajmr2021.9580","DOIUrl":"https://doi.org/10.5897/ajmr2021.9580","url":null,"abstract":"","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44772199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bi Rosin Voko Don-Rodrigue, C. K. Kouassi, Athanase Kouassi Kra, Konaté Ibrahim
Ivorian forest has been experiencing degradation for several decades despite reforestation efforts. The main cause of the failure of reforestation policies is an important mortality at the time of planting (transplanting stress). To remedy this problem, mycorrhization technology based on the beneficial effects of arbuscular mycorrhizal fungi (AMF) could provide a sustainable solution. The objective of this study was to evaluate the effects of AMF inocula (local and commercial inoculum) on the juvenile growth of a Côte d'Ivoire forest species ( Guibourtia ehie ). Vegetative growth parameters and mineral nutrition (N, P, K, and Ca) were evaluated. After 150 days of cultivation, the plants treated with the local inoculum had the highest mycorrhization frequencies (75%) and intensities (21.23%). Also for growth parameters (height, number of leaves, leaf area, and crown diameter) and for nitrogen and potassium contents, the plants treated with the local polyspecific inoculum had the highest values compared to the plants treated with the commercial inoculum and the control plants. Mycorrhization improved mineral nutrition as well as vegetative growth of G. ehie seedlings. The integration of mycorrhizal inoculation from local strains in reforestation policies could be a sustainable solution for the recolonization of degraded forests by endangered species.
{"title":"Impact of mycorrhization on transplanting stress and the juvenile growth of an Ivorian forest species Guibourtia ehie (Fabaceae, (A. Chev.) J. Leonard)","authors":"Bi Rosin Voko Don-Rodrigue, C. K. Kouassi, Athanase Kouassi Kra, Konaté Ibrahim","doi":"10.5897/ajmr2021.9542","DOIUrl":"https://doi.org/10.5897/ajmr2021.9542","url":null,"abstract":"Ivorian forest has been experiencing degradation for several decades despite reforestation efforts. The main cause of the failure of reforestation policies is an important mortality at the time of planting (transplanting stress). To remedy this problem, mycorrhization technology based on the beneficial effects of arbuscular mycorrhizal fungi (AMF) could provide a sustainable solution. The objective of this study was to evaluate the effects of AMF inocula (local and commercial inoculum) on the juvenile growth of a Côte d'Ivoire forest species ( Guibourtia ehie ). Vegetative growth parameters and mineral nutrition (N, P, K, and Ca) were evaluated. After 150 days of cultivation, the plants treated with the local inoculum had the highest mycorrhization frequencies (75%) and intensities (21.23%). Also for growth parameters (height, number of leaves, leaf area, and crown diameter) and for nitrogen and potassium contents, the plants treated with the local polyspecific inoculum had the highest values compared to the plants treated with the commercial inoculum and the control plants. Mycorrhization improved mineral nutrition as well as vegetative growth of G. ehie seedlings. The integration of mycorrhizal inoculation from local strains in reforestation policies could be a sustainable solution for the recolonization of degraded forests by endangered species.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43560938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Wanjala, L. Odokuma, I. Etela, R. Ramkat, B. Odogwu
1 Department of Microbiology, Faculty of Science, University of Port Harcourt, Nigeria. 2 Department of Animal Science, Faculty of Agriculture, University of Port Harcourt, Nigeria. 3 Center of Excellence in Phytochemicals, Textiles and Renewable Energy, Faculty of Science, Moi University, Eldoret, Kenya. 4 Department of Plant Science and Biochemistry, Faculty of Science, University of Port Harcourt, Nigeria. 5 Department of Physical and Biological Sciences, Faculty of Science, Bomet University College, Kenya.
{"title":"Characterization of culturable microbial community in oil contaminated soils in Greater Port Harcourt Area, Nigeria","authors":"M. Wanjala, L. Odokuma, I. Etela, R. Ramkat, B. Odogwu","doi":"10.5897/ajmr2020.9398","DOIUrl":"https://doi.org/10.5897/ajmr2020.9398","url":null,"abstract":"1 Department of Microbiology, Faculty of Science, University of Port Harcourt, Nigeria. 2 Department of Animal Science, Faculty of Agriculture, University of Port Harcourt, Nigeria. 3 Center of Excellence in Phytochemicals, Textiles and Renewable Energy, Faculty of Science, Moi University, Eldoret, Kenya. 4 Department of Plant Science and Biochemistry, Faculty of Science, University of Port Harcourt, Nigeria. 5 Department of Physical and Biological Sciences, Faculty of Science, Bomet University College, Kenya.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46034006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Olumuyiwa Balogun Olasinbo, C. Sylvanus, O. Peters
High global prevalence of multidrug-resistant bacteria due to antibiotics misuse has prompted the need for novel antibacterial compounds to replace the failing antibiotics. This study investigated some natural habitats in Abuja, Nigeria, for antibiotics-producing bacteria. Thirty-six soil samples from termite mounds, river banks and rhizospheres of Anacardium occidentale L. (cashew tree), Gmelina arborea Roxb. ex Sm. (beechwood), Ageratum conyzoides L. (goat weed) including Cymbopogon citratus (DC) Stapf. (lemon grass) were cultured on nutrient media. Twelve potential antibiotic-producing isolates were identified by crowded plates method and characterized using Bergey’s manual. The antimicrobial activities of the filtrates from the isolates against some pathogenic strains namely Streptococcus pneumonia, Salmonella typhi, Escherichia coli (ATCC 25922), Proteus mirabilis, Staphylococcus aureus (ATCC 25923), Candida albicans, Bacillus subtilis and Pseudomonas aeruginosa (ATCC 27853) were determined by agar-well diffusion method. Only 5 eventually inhibited at least 1 test microorganism; 4 showed activities against both Gram-positive and Gram-negative bacteria (broad spectrum) and 2 among the 4 also inhibited Candida albicans , while the remaining 1 inhibited only 1 Gram-positive bacterium (narrow spectrum). The 5 potent antibiotics-producers were Bacillus spp. In conclusion, some natural habitats in the FCT are important sources of antibiotic-producing bacteria. Their antimicrobial lead compounds could be extracted and developed locally for pharmaceutical applications.
{"title":"Antibiotic-producing bacteria isolated from some natural habitats in the Federal Capital Territory (FCT), Nigeria","authors":"Olumuyiwa Balogun Olasinbo, C. Sylvanus, O. Peters","doi":"10.5897/ajmr2021.9587","DOIUrl":"https://doi.org/10.5897/ajmr2021.9587","url":null,"abstract":"High global prevalence of multidrug-resistant bacteria due to antibiotics misuse has prompted the need for novel antibacterial compounds to replace the failing antibiotics. This study investigated some natural habitats in Abuja, Nigeria, for antibiotics-producing bacteria. Thirty-six soil samples from termite mounds, river banks and rhizospheres of Anacardium occidentale L. (cashew tree), Gmelina arborea Roxb. ex Sm. (beechwood), Ageratum conyzoides L. (goat weed) including Cymbopogon citratus (DC) Stapf. (lemon grass) were cultured on nutrient media. Twelve potential antibiotic-producing isolates were identified by crowded plates method and characterized using Bergey’s manual. The antimicrobial activities of the filtrates from the isolates against some pathogenic strains namely Streptococcus pneumonia, Salmonella typhi, Escherichia coli (ATCC 25922), Proteus mirabilis, Staphylococcus aureus (ATCC 25923), Candida albicans, Bacillus subtilis and Pseudomonas aeruginosa (ATCC 27853) were determined by agar-well diffusion method. Only 5 eventually inhibited at least 1 test microorganism; 4 showed activities against both Gram-positive and Gram-negative bacteria (broad spectrum) and 2 among the 4 also inhibited Candida albicans , while the remaining 1 inhibited only 1 Gram-positive bacterium (narrow spectrum). The 5 potent antibiotics-producers were Bacillus spp. In conclusion, some natural habitats in the FCT are important sources of antibiotic-producing bacteria. Their antimicrobial lead compounds could be extracted and developed locally for pharmaceutical applications.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47690456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Over the years, snacking has become a regular habit for majority of the population with increasing need for food manufacturers to meet consumers’ demand through product development. In this study, snack bars were prepared using maize and Bambara nuts mixed in the following ratios: A, 100% maize; B, 100% Bambara nuts; C, 50% maize: 50% Bambara nuts; D, 75% maize: 25% Bambara nuts; E, 75% Bambara nuts: 25% maize. The total heterotrophic bacterial count of samples A to E was within the limit stipulated by International Commission on Microbiological Specification for Food. The frequency of occurrence of bacterial isolates from the samples include Bacillus species (24%), Staphylococcus species (24%), Lactobacillus species (24%), Escherichia coli (19%) and Serratia species (9%), while the fungal isolates include Aspergillus (33%), Penicillium (27%), Rhizopus (20%) and Saccharomyces species (20%). The moisture, ash, carbohydrate, crude protein, fat and fiber content of the samples were within the range of 11.47±0.99-17.45±1.01, 1.09±0.07-2.00±0.15, 56.05±0.65-70.37±0.71, 6.32±0.36-15.00±0.22, 4.60±0.50-7.00±0.30 and 2.60±0.25-3.10±0.31%, respectively. There was a significant difference (p<0.05) in the proximate composition among the samples except for crude fiber. The calorie value range between 347.20-367.69 kcal and acceptability of the samples compared favourably with a commercialized snack bar.
{"title":"Microbiological, nutritional and sensory evaluation of snack bars developed using Bambara groundnut (Vigna subterranean L.) and maize (Zea mays)","authors":"Ahaotu Ihuoma, Okechukwu Ichendu Marvellous, Maduka Ndukwe","doi":"10.5897/ajmr2021.9583","DOIUrl":"https://doi.org/10.5897/ajmr2021.9583","url":null,"abstract":"Over the years, snacking has become a regular habit for majority of the population with increasing need for food manufacturers to meet consumers’ demand through product development. In this study, snack bars were prepared using maize and Bambara nuts mixed in the following ratios: A, 100% maize; B, 100% Bambara nuts; C, 50% maize: 50% Bambara nuts; D, 75% maize: 25% Bambara nuts; E, 75% Bambara nuts: 25% maize. The total heterotrophic bacterial count of samples A to E was within the limit stipulated by International Commission on Microbiological Specification for Food. The frequency of occurrence of bacterial isolates from the samples include Bacillus species (24%), Staphylococcus species (24%), Lactobacillus species (24%), Escherichia coli (19%) and Serratia species (9%), while the fungal isolates include Aspergillus (33%), Penicillium (27%), Rhizopus (20%) and Saccharomyces species (20%). The moisture, ash, carbohydrate, crude protein, fat and fiber content of the samples were within the range of 11.47±0.99-17.45±1.01, 1.09±0.07-2.00±0.15, 56.05±0.65-70.37±0.71, 6.32±0.36-15.00±0.22, 4.60±0.50-7.00±0.30 and 2.60±0.25-3.10±0.31%, respectively. There was a significant difference (p<0.05) in the proximate composition among the samples except for crude fiber. The calorie value range between 347.20-367.69 kcal and acceptability of the samples compared favourably with a commercialized snack bar.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49235211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Wei, Xiulian Miao, Yue Tian, Jing Du, Meng Wang
Springtails are considered as an important candidate bioindicator to assess soil quality spiked with trace metals, but little is known of their endophytic bacteria. In this study, a kind of Tomoceridae springtail was used, and a total of 45 effective sequences were obtained through the process of endophytic bacteria isolation, culture, polymerase chain reaction (PCR) amplification and sequencing. After NCBI-BLAST, the results showed that there were 20 bacterial colonies belonging to the genus Staphylococcus, 12 belonging to the genus Bacillus, 7 belonging to the genus Paenibacillus, 1 belonging to the genus Exiguobacterium and 5 belonging to Acinetobacter lwoffii. Furthermore, five bacterial strains from these five genera (named TomoRZH14, TomoRZH26, TomoRZH30, TomoRZH37, TomoRZH40) were selected for cellulose degradation analysis. The results showed that TomoRZH26 (Bacillus sp.) seemed to have a stronger cellulose degradation ability than those of the other four strains, while the three main components cellulase endo-β-glucanase, exo-β-glucanase and βglucosidase in TomoRZH26 showed significantly higher enzymatic activity than in the other strains. Viscosity analysis also showed that the TomoRZH26 bacterium degraded relatively quickly in cellulase fermentation medium. In general, in this study, we preliminarily revealed several endophytic bacteria of Tomoceridae springtails and found that they had potentially strong cellulose degradation activity, which may be one of the important reasons behind springtail adaptation to this kind of soil ecological environment.
{"title":"Isolation and identification of cellulose-degrading endophytic bacteria from Tomoceridae (springtails)","authors":"D. Wei, Xiulian Miao, Yue Tian, Jing Du, Meng Wang","doi":"10.5897/ajmr2021.9582","DOIUrl":"https://doi.org/10.5897/ajmr2021.9582","url":null,"abstract":"Springtails are considered as an important candidate bioindicator to assess soil quality spiked with trace metals, but little is known of their endophytic bacteria. In this study, a kind of Tomoceridae springtail was used, and a total of 45 effective sequences were obtained through the process of endophytic bacteria isolation, culture, polymerase chain reaction (PCR) amplification and sequencing. After NCBI-BLAST, the results showed that there were 20 bacterial colonies belonging to the genus Staphylococcus, 12 belonging to the genus Bacillus, 7 belonging to the genus Paenibacillus, 1 belonging to the genus Exiguobacterium and 5 belonging to Acinetobacter lwoffii. Furthermore, five bacterial strains from these five genera (named TomoRZH14, TomoRZH26, TomoRZH30, TomoRZH37, TomoRZH40) were selected for cellulose degradation analysis. The results showed that TomoRZH26 (Bacillus sp.) seemed to have a stronger cellulose degradation ability than those of the other four strains, while the three main components cellulase endo-β-glucanase, exo-β-glucanase and βglucosidase in TomoRZH26 showed significantly higher enzymatic activity than in the other strains. Viscosity analysis also showed that the TomoRZH26 bacterium degraded relatively quickly in cellulase fermentation medium. In general, in this study, we preliminarily revealed several endophytic bacteria of Tomoceridae springtails and found that they had potentially strong cellulose degradation activity, which may be one of the important reasons behind springtail adaptation to this kind of soil ecological environment.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42350397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Tanni, M. Nesa, R. Kabir, F. Habib, R. Zaman, N. Tania, A. Haque, A. Chowdhury, N. Sharmin, K. Halder, M. Chowdhury, M. Rahman, S. Shahid, S. Nahar, S. Shamsuzzaman
Oxford AstraZeneca (Covishield) vaccine is the 1st vaccine administered in Bangladesh to prevent the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The antibody response after 1st and 2nd doses of this vaccine was assessed in health care workers of Dhaka Medical College Hospital, Bangladesh. Blood sample was collected from healthcare workers (teachers, clinicians and medical staff) after 28 days of 1st vaccination and 14 days after 2nd vaccination. Quantitative post-vaccination antibody responses were measured using the chemiluminescent immunoassay, ADVIA Centaur (Siemens, Munich, Germany) SARS-CoV-2 IgG (COV2G) assay (output index was 1.00). Vaccine related antibody was found in 126 (41%) participants after 1st dose of AstraZeneca vaccine. After 2nd dose of vaccine, reactive level of antibody was found in 172 (93%) participants. Antibody responses were significantly higher in previously infected participants compared to participants who had no history of previous COVID-19 after 1st dose (51.92+or-50.85 vs 23.67+or-41.07, p=0.001) as well as 2nd dose of vaccine (64.12+or-97.76 vs 35.04+or-64.84, p=0.001). No difference in antibody response was observed among participants with or without comorbidities. Oxford AstraZeneca Covishield vaccine induces a strong immune response after two doses of vaccination.
牛津阿斯利康(Covishield)疫苗是孟加拉国为防止严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)传播而接种的第一种疫苗。在孟加拉国达卡医学院医院的卫生保健工作者中评估了该疫苗第一剂和第二剂后的抗体反应。卫生保健工作者(教师、临床医生和医务人员)在第一次接种疫苗28天后和第二次接种疫苗14天后采集血样。采用化学发光免疫分析法，ADVIA Centaur (Siemens, Munich, Germany) SARS-CoV-2 IgG (COV2G)测定法(输出指数为1.00)，定量测定疫苗接种后抗体应答。126名(41%)参与者在第一次接种阿斯利康疫苗后发现疫苗相关抗体。在第2次接种后，172例(93%)参与者的抗体出现反应性水平。在第一次接种疫苗(51.92+or-50.85 vs 23.67+or-41.07, p=0.001)和第二次接种疫苗(64.12+or-97.76 vs 35.04+or-64.84, p=0.001)后，先前感染的参与者的抗体反应明显高于之前没有COVID-19病史的参与者。在有或没有合并症的参与者中，没有观察到抗体反应的差异。牛津阿斯利康Covishield疫苗诱导强烈的免疫反应后，两剂疫苗接种。
{"title":"Antibody responses after Oxford AstraZeneca (Covishield) vaccine among healthcare workers in Dhaka Medical College, Dhaka, Bangladesh","authors":"N. Tanni, M. Nesa, R. Kabir, F. Habib, R. Zaman, N. Tania, A. Haque, A. Chowdhury, N. Sharmin, K. Halder, M. Chowdhury, M. Rahman, S. Shahid, S. Nahar, S. Shamsuzzaman","doi":"10.5897/AJMR2021.9590","DOIUrl":"https://doi.org/10.5897/AJMR2021.9590","url":null,"abstract":"Oxford AstraZeneca (Covishield) vaccine is the 1st vaccine administered in Bangladesh to prevent the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The antibody response after 1st and 2nd doses of this vaccine was assessed in health care workers of Dhaka Medical College Hospital, Bangladesh. Blood sample was collected from healthcare workers (teachers, clinicians and medical staff) after 28 days of 1st vaccination and 14 days after 2nd vaccination. Quantitative post-vaccination antibody responses were measured using the chemiluminescent immunoassay, ADVIA Centaur (Siemens, Munich, Germany) SARS-CoV-2 IgG (COV2G) assay (output index was 1.00). Vaccine related antibody was found in 126 (41%) participants after 1st dose of AstraZeneca vaccine. After 2nd dose of vaccine, reactive level of antibody was found in 172 (93%) participants. Antibody responses were significantly higher in previously infected participants compared to participants who had no history of previous COVID-19 after 1st dose (51.92+or-50.85 vs 23.67+or-41.07, p=0.001) as well as 2nd dose of vaccine (64.12+or-97.76 vs 35.04+or-64.84, p=0.001). No difference in antibody response was observed among participants with or without comorbidities. Oxford AstraZeneca Covishield vaccine induces a strong immune response after two doses of vaccination.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71092669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sina Haziz, N. Christine, D. Durand, Gnama-Tchao Gnimdou, B. Bawa, Socohou Akim, Ramanou Alao Sanni Abdou, Baba-Moussa Farid, A. Adolphe, B. Lamine
The aim of this study is to determine the distribution of the genotypic pathogenicity traits of vaginal high-risk infectious bacteria (HRIB) collected in the CHU-MEL of Cotonou (Benin). To achieve this, a recto-vaginal swab of 42 pregnant women in the third trimester of pregnancy was collected. Species identification was carried out by specific biochemical tests. Antimicrobial susceptibility was tested according to the microbiology standard recommendation. Macrolide resistance genes in Gram-positive bacteria and virulence genes in Escherichia coli were investigated by polymerase chain reaction (PCR). E. coli is the most isolated species (14.7%) followed by Klebsiella pneumoniae (11.8%). Mono-microbial carriage was 55.9%. Gram-negative antibiotic susceptibility shows strong resistance to beta lactam. While Gram-positive bacteria showed strong resistance to beta-lactamine, tetracycline and macrolides with cMLS B (70.4%), iMLS B (3.7%) and M (25.9%) phenotypes. ErmB and ermTR were not detected in Gram-positive bacteria but mef(A/E) was detected at a high. Virulence genes in E. coli were detected and fimA was the most common (52.2%) followed by sfa/foc (30.4%) and cnf1 (13.0%). NeuC and ibeA have not been detected. The hvgA virulence gene was detected in S. agalactiae at a rate of 61.54%. These results demonstrate the importance of introducing antenatal screening for HRIB to improve obstetric and neonatal management in Benin.
{"title":"Molecular characterization of high-risk infection vaginal bacteria isolated from pregnant women in CHU-MEL of Cotonou (Benin)","authors":"Sina Haziz, N. Christine, D. Durand, Gnama-Tchao Gnimdou, B. Bawa, Socohou Akim, Ramanou Alao Sanni Abdou, Baba-Moussa Farid, A. Adolphe, B. Lamine","doi":"10.5897/ajmr2021.9589","DOIUrl":"https://doi.org/10.5897/ajmr2021.9589","url":null,"abstract":"The aim of this study is to determine the distribution of the genotypic pathogenicity traits of vaginal high-risk infectious bacteria (HRIB) collected in the CHU-MEL of Cotonou (Benin). To achieve this, a recto-vaginal swab of 42 pregnant women in the third trimester of pregnancy was collected. Species identification was carried out by specific biochemical tests. Antimicrobial susceptibility was tested according to the microbiology standard recommendation. Macrolide resistance genes in Gram-positive bacteria and virulence genes in Escherichia coli were investigated by polymerase chain reaction (PCR). E. coli is the most isolated species (14.7%) followed by Klebsiella pneumoniae (11.8%). Mono-microbial carriage was 55.9%. Gram-negative antibiotic susceptibility shows strong resistance to beta lactam. While Gram-positive bacteria showed strong resistance to beta-lactamine, tetracycline and macrolides with cMLS B (70.4%), iMLS B (3.7%) and M (25.9%) phenotypes. ErmB and ermTR were not detected in Gram-positive bacteria but mef(A/E) was detected at a high. Virulence genes in E. coli were detected and fimA was the most common (52.2%) followed by sfa/foc (30.4%) and cnf1 (13.0%). NeuC and ibeA have not been detected. The hvgA virulence gene was detected in S. agalactiae at a rate of 61.54%. These results demonstrate the importance of introducing antenatal screening for HRIB to improve obstetric and neonatal management in Benin.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46225959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Milton Coutinho Salomão Maira, Santana Soares e Barros Ludmilla
Although in 2020 the country's Gross Domestic Product (GDP) declined 4.1% compared to the previous year, the participation of livestock in the same period increased its representation from 8.4 to 10%. It is estimated that Brazil's contribution to the world food market has grown from 20.6 billion to 100 billion and the prospects are for greater increases for the coming years. In terms of people fed, the numbers were 800 million people. Thus, it is important to avoid the occurrence of illnesses, especially those transmitted by food. Included in this group are zoonosis, defined as diseases transmitted by animals to humans, which can be bacterial, viral or parasitic. According to the World Organization for Animal Health, 60% of human infectious diseases are zoonotic and 75% of emerging infectious disease agents in man is of animal origin. Among the main ones are the taeniasis-cysticercosis complex and tuberculosis. They are a global public health problem and prevent the efficient production of food. This work is a literature review that aimed to discuss the causative agents of these diseases, as well as the characteristics, contamination in food and possible measures for its control.
{"title":"Taeniasis-cysticercosis complex and tuberculosis in food","authors":"Milton Coutinho Salomão Maira, Santana Soares e Barros Ludmilla","doi":"10.5897/ajmr2021.9578","DOIUrl":"https://doi.org/10.5897/ajmr2021.9578","url":null,"abstract":"Although in 2020 the country's Gross Domestic Product (GDP) declined 4.1% compared to the previous year, the participation of livestock in the same period increased its representation from 8.4 to 10%. It is estimated that Brazil's contribution to the world food market has grown from 20.6 billion to 100 billion and the prospects are for greater increases for the coming years. In terms of people fed, the numbers were 800 million people. Thus, it is important to avoid the occurrence of illnesses, especially those transmitted by food. Included in this group are zoonosis, defined as diseases transmitted by animals to humans, which can be bacterial, viral or parasitic. According to the World Organization for Animal Health, 60% of human infectious diseases are zoonotic and 75% of emerging infectious disease agents in man is of animal origin. Among the main ones are the taeniasis-cysticercosis complex and tuberculosis. They are a global public health problem and prevent the efficient production of food. This work is a literature review that aimed to discuss the causative agents of these diseases, as well as the characteristics, contamination in food and possible measures for its control.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46952446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：