M. Yassine, B. Malika, Messafeur Abdelkrim, Matmour Derouicha, Belmokhtar Zoubir, Derrar Hichem, Djaroud Samira, Zohra Benaissa Fatima
our study is the identification of the fungal species colonizing surfaces of dental appliances, the cross-sectional study was carried out on 60 patients wearing dental appliances, during 5 months, and sterile swabs were used and inoculated into Sabouraud’s dextrose agar tubes. Yeast identification has been based on germ tube test, chlamydoconidia production and biochimical tests (Auxacolor, Api 20 C). The overall prevalence of fungal contamination of dental orthodontic appliance was 35%. The prevalence rate was 40% among patients who had dental appliances for 1 to 2 months, and 45,5% fort patients brushing their teeth at least twice daily, but no statistical relation with mycosis was found. Pain, burning and discomfort were correlated to fungal carriage p=0,038; OR=4,867 IC 95% (1.09-21.74). The fungal species most recovered were Candida parapsilosis 21.47%, Candida Zeylanoides 21.47%, Candida albicans 17.38%, and Cryptococcus terreus 13.04%. Mycological monitoring of dental prosthesis is crucial to prevent possible fungal’s adverse health effects. the
我们的研究是鉴定定植在牙科器具表面的真菌种类，对60名佩戴牙科器具的患者进行了横断面研究，为期5个月，使用无菌拭子接种到Sabouraud 's葡萄糖琼脂管中。根据试管试验、衣原体生产和生化试验(Auxacolor, Api 20 C)对酵母菌进行鉴定，正畸矫治器真菌污染的总体发生率为35%。使用牙具1 ~ 2个月的患者患病率为40%，每天至少刷牙两次的患者患病率为45.5%，但与真菌病无统计学关系。疼痛、灼烧和不适与真菌携带相关p= 0.038;Or =4,867 IC 95%(1.09-21.74)。检出最多的真菌种类依次为假丝酵母菌21.47%、zeylanides假丝酵母菌21.47%、白色假丝酵母菌17.38%和地隐球菌13.04%。口腔修复体的真菌学监测对于预防真菌对健康的不良影响至关重要。的
{"title":"Fungal contamination of dental appliances: A cross- sectional study","authors":"M. Yassine, B. Malika, Messafeur Abdelkrim, Matmour Derouicha, Belmokhtar Zoubir, Derrar Hichem, Djaroud Samira, Zohra Benaissa Fatima","doi":"10.5897/ajmr2021.9569","DOIUrl":"https://doi.org/10.5897/ajmr2021.9569","url":null,"abstract":"our study is the identification of the fungal species colonizing surfaces of dental appliances, the cross-sectional study was carried out on 60 patients wearing dental appliances, during 5 months, and sterile swabs were used and inoculated into Sabouraud’s dextrose agar tubes. Yeast identification has been based on germ tube test, chlamydoconidia production and biochimical tests (Auxacolor, Api 20 C). The overall prevalence of fungal contamination of dental orthodontic appliance was 35%. The prevalence rate was 40% among patients who had dental appliances for 1 to 2 months, and 45,5% fort patients brushing their teeth at least twice daily, but no statistical relation with mycosis was found. Pain, burning and discomfort were correlated to fungal carriage p=0,038; OR=4,867 IC 95% (1.09-21.74). The fungal species most recovered were Candida parapsilosis 21.47%, Candida Zeylanoides 21.47%, Candida albicans 17.38%, and Cryptococcus terreus 13.04%. Mycological monitoring of dental prosthesis is crucial to prevent possible fungal’s adverse health effects. the","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48892936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study determined the prevalence and drug resistant patterns of bacteria isolated from untreated hospital wastewaters collected from selected hospitals in Offa Local Government Area of Kwara State, Nigeria. A total of 42 composite samples were aseptically collected, transported and analyzed for enumeration of microorganisms, bacteriological identification and susceptibility testing following standard procedures. The Global Positioning System (GPS) coordinates of each site location was equally taken and data obtained were analyzed using SPSS version 20. The means bacterial count population of wet season samples ranged between 7±4.00 × 10 5 and 150±43.59 × (10 5 cfu/ml), while that of dry season samples ranged between 10±2.00 × 10 5 and 225±67.27 × 10 5 cfu/ml. Among the total samples, 50 bacterial isolates were detected, of which 26(52%) were from wet season samples and 24(48%) were from dry season samples. The most frequently isolated bacteria from wet season samples was Alcaligenes faecalis 17(65.4%) followed by Alcaligenes aquatilis 5(19.2%) and Staphylococcus saprophyticus 4(15.4%). Findings from antibiotic resistance pattern of the isolates indicated that ofloxacin (OFL) demonstrated highest antimicrobial potency against the test isolates, with Zone inhibition diameters (mm) (resistant ≤12, intermediate 13-15 and susceptible ≥ 16). Thus, hospital wastewater should be treated before discharge to prevent infectious diseases.
{"title":"Antibiotic resistant pattern of bacteria in untreated hospital wastewaters from Offa Local Government Area, Kwara State, Nigeria","authors":"K. M. Usman, D. Arotupin, F. Ekundayo","doi":"10.5897/ajmr2021.9584","DOIUrl":"https://doi.org/10.5897/ajmr2021.9584","url":null,"abstract":"This study determined the prevalence and drug resistant patterns of bacteria isolated from untreated hospital wastewaters collected from selected hospitals in Offa Local Government Area of Kwara State, Nigeria. A total of 42 composite samples were aseptically collected, transported and analyzed for enumeration of microorganisms, bacteriological identification and susceptibility testing following standard procedures. The Global Positioning System (GPS) coordinates of each site location was equally taken and data obtained were analyzed using SPSS version 20. The means bacterial count population of wet season samples ranged between 7±4.00 × 10 5 and 150±43.59 × (10 5 cfu/ml), while that of dry season samples ranged between 10±2.00 × 10 5 and 225±67.27 × 10 5 cfu/ml. Among the total samples, 50 bacterial isolates were detected, of which 26(52%) were from wet season samples and 24(48%) were from dry season samples. The most frequently isolated bacteria from wet season samples was Alcaligenes faecalis 17(65.4%) followed by Alcaligenes aquatilis 5(19.2%) and Staphylococcus saprophyticus 4(15.4%). Findings from antibiotic resistance pattern of the isolates indicated that ofloxacin (OFL) demonstrated highest antimicrobial potency against the test isolates, with Zone inhibition diameters (mm) (resistant ≤12, intermediate 13-15 and susceptible ≥ 16). Thus, hospital wastewater should be treated before discharge to prevent infectious diseases.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43045467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
1 Laboratoire National de Biologie Clinique et de Santé Publique, Bangui, Central African Republic. 2 Faculté des Sciences de la Santé, Université de Bangui, Bangui, Central African Republic. 3 Ecole doctorale des Sciences et Techniques, Faculté des Sciences, Université de Bangui, Bangui, Central African Republic. 4 Laboratoire de Bactériologie-Virologie du Centre Hospitalier Universitaire de Brazzaville, Republic of Congo. 5 Faculté des Sciences de la Santé de l’Université Marien NGOUABI de Brazzaville, Republic of Congo.
{"title":"Diagnostic value of two HIV rapid diagnostic tests 16 years after their adoption in national strategies in the Central African Republic (CAR)","authors":"Diamant Mossoro-Kpinde Christian, Mbesse Thierry, Bobossi Christelle, Bokia-Baguida Coretha, Arthur Sombot Simplice, Esther Ontsira Ngoyi Nina, Dahlia Mossoro-Kpinde Hermione, Feissona Rosine, G. Gérard","doi":"10.5897/ajmr2021.9557","DOIUrl":"https://doi.org/10.5897/ajmr2021.9557","url":null,"abstract":"1 Laboratoire National de Biologie Clinique et de Santé Publique, Bangui, Central African Republic. 2 Faculté des Sciences de la Santé, Université de Bangui, Bangui, Central African Republic. 3 Ecole doctorale des Sciences et Techniques, Faculté des Sciences, Université de Bangui, Bangui, Central African Republic. 4 Laboratoire de Bactériologie-Virologie du Centre Hospitalier Universitaire de Brazzaville, Republic of Congo. 5 Faculté des Sciences de la Santé de l’Université Marien NGOUABI de Brazzaville, Republic of Congo.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45578455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Meningitis is a public health concern. It is caused by several etiologic agents that vary by age group and geographical area. This study aims to highlight the etiological and predictive factors of acute meningitis in hospitalized febrile patients in Mansoura Fever Hospital, Egypt. This study includes cases admitted with suspected meningitis. The study is conducted in the period between April 2019 and March 2020. Lumbar puncture, CSF examination and blood culture and sensitivity were done. Brain Magnetic Resonance Imaging (MRI) was performed before lumbar puncture in some patients. Detailed analysis of epidemiologic characteristics, clinical data, laboratory findings, the causative organisms and predictors of patients with Bacterial Meningitis (BM) were studied. This study included 110 patients had BM with CSF leukocytosis > 100 cells/mm3. Out of 110 CSF samples, 95 cases (86.4%) pathogens were detected by direct Gram-stained smear. Gram positive cocci were the commonest microorganism isolated. 66.4% of patients had blood culture growth of the same organism as the CSF culture. Reagent strip CSF examinations showed a positive correlation compared with laboratory tests. BM had 22.7% mortality rate. Predictive factors of poor outcomes include CSF/serum glucose ratio >0.6, CSF protein >80 mg/dl and Tonsillitis. Prognostic factors that are associated with poor outcome include old age, late presentation, delayed antibiotics treatment, neurologic complications and Glasgo Coma Scale (GCS). BM remains a leading cause of morbidity and mortality,so early diagnosis and treatment decrease both. Predictors of poor outcome of BM are CSF/serum glucose ratio >0.6, CSF protein >80 mg/dl and Tonsillitis.
{"title":"Etiological and predictive factors of Acute Meningitis in Mansoura fever hospital, Egypt","authors":"M. Sameh, A. Hassan, M. Aya, A. Naglaa, L. Ahmed","doi":"10.5897/ajmr2021.9576","DOIUrl":"https://doi.org/10.5897/ajmr2021.9576","url":null,"abstract":"Meningitis is a public health concern. It is caused by several etiologic agents that vary by age group and geographical area. This study aims to highlight the etiological and predictive factors of acute meningitis in hospitalized febrile patients in Mansoura Fever Hospital, Egypt. This study includes cases admitted with suspected meningitis. The study is conducted in the period between April 2019 and March 2020. Lumbar puncture, CSF examination and blood culture and sensitivity were done. Brain Magnetic Resonance Imaging (MRI) was performed before lumbar puncture in some patients. Detailed analysis of epidemiologic characteristics, clinical data, laboratory findings, the causative organisms and predictors of patients with Bacterial Meningitis (BM) were studied. This study included 110 patients had BM with CSF leukocytosis > 100 cells/mm3. Out of 110 CSF samples, 95 cases (86.4%) pathogens were detected by direct Gram-stained smear. Gram positive cocci were the commonest microorganism isolated. 66.4% of patients had blood culture growth of the same organism as the CSF culture. Reagent strip CSF examinations showed a positive correlation compared with laboratory tests. BM had 22.7% mortality rate. Predictive factors of poor outcomes include CSF/serum glucose ratio >0.6, CSF protein >80 mg/dl and Tonsillitis. Prognostic factors that are associated with poor outcome include old age, late presentation, delayed antibiotics treatment, neurologic complications and Glasgo Coma Scale (GCS). BM remains a leading cause of morbidity and mortality,so early diagnosis and treatment decrease both. Predictors of poor outcome of BM are CSF/serum glucose ratio >0.6, CSF protein >80 mg/dl and Tonsillitis.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47540813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
1 Unit of Epidemic-Prone Diseases, Emerging Diseases and Zoonosis (UMEMEZ), National Reference Laboratory for Influenza (LNR-G), Department of Biomedical and Public Health, Health Science Research Institute (IRSS), National Centre for Scientific and Technological Research (CNRST), 03 BP 7192 Ouagadougou 03, Burkina Faso. 2 Department Animal Health, National School of Livestock and Animal Health (ENESA), 03 BP 7026 Ouagadougou 03, Burkina Faso.
1 .易流行疾病、新发疾病和人畜共患病国家流感参考实验室，生物医学和公共卫生系，卫生科学研究所，国家科学和技术研究中心，瓦加杜古03 BP 7192，布基纳法索。2国家牲畜和动物卫生学院动物卫生系，瓦加杜古03 BP 7026，布基纳法索。
{"title":"Seroprevalence and behaviour at risk of zoonotic transmission of bovine brucellosis in Namentenga Province, Burkina Faso","authors":"Tialla Dieudonné","doi":"10.5897/ajmr2021.9579","DOIUrl":"https://doi.org/10.5897/ajmr2021.9579","url":null,"abstract":"1 Unit of Epidemic-Prone Diseases, Emerging Diseases and Zoonosis (UMEMEZ), National Reference Laboratory for Influenza (LNR-G), Department of Biomedical and Public Health, Health Science Research Institute (IRSS), National Centre for Scientific and Technological Research (CNRST), 03 BP 7192 Ouagadougou 03, Burkina Faso. 2 Department Animal Health, National School of Livestock and Animal Health (ENESA), 03 BP 7026 Ouagadougou 03, Burkina Faso.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46066233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thyme is one of the most important medicinal plants in wild rangeland in Iran that has lots of benefits. Pseudomonas flourescence is one of the effective plant growth promoting bacteria (PGPR) as a bioinoculant for medicinal plants. This study aims to survey three inoculation techniques with PGPR on growth and oil content in Thymus kotschyanus for organic cultivation. For this research, an experiment was conducted in Randomized Complete Block Design at Research Institute Forest and Rangelands, Tehran, Iran, with four treatments and three replications. In the first method, thyme seed was treated in liquid bacterial suspension for 3 h, in the second method bacterial suspension injected around the root before cultivation in farm and in the third method both seed and root have been treated by the bacterial suspension. In all three methods of inoculation of P. fluorescence, an increase in growth and the amount of essential oil was determined. The highest amount of root volume (30 mL) compared to the control (15 mL) was significant at the level of P=0.05 and the highest amount of essential oil (1.74%) was obtained by using the third inoculation method. It could be concluded that the microbial inoculation method has a great influence on the yield of T. kotschyanus and bacterial inoculation of seeds and roots had a greater effect, rather than inoculated separately for organic cultivation.
{"title":"Survey on efficiency of inoculation methods of Pseudomonas fluorescens on growth and yield of Thymus kotschyanus","authors":"S. Ali, M. Mohammad","doi":"10.5897/ajmr2021.9574","DOIUrl":"https://doi.org/10.5897/ajmr2021.9574","url":null,"abstract":"Thyme is one of the most important medicinal plants in wild rangeland in Iran that has lots of benefits. Pseudomonas flourescence is one of the effective plant growth promoting bacteria (PGPR) as a bioinoculant for medicinal plants. This study aims to survey three inoculation techniques with PGPR on growth and oil content in Thymus kotschyanus for organic cultivation. For this research, an experiment was conducted in Randomized Complete Block Design at Research Institute Forest and Rangelands, Tehran, Iran, with four treatments and three replications. In the first method, thyme seed was treated in liquid bacterial suspension for 3 h, in the second method bacterial suspension injected around the root before cultivation in farm and in the third method both seed and root have been treated by the bacterial suspension. In all three methods of inoculation of P. fluorescence, an increase in growth and the amount of essential oil was determined. The highest amount of root volume (30 mL) compared to the control (15 mL) was significant at the level of P=0.05 and the highest amount of essential oil (1.74%) was obtained by using the third inoculation method. It could be concluded that the microbial inoculation method has a great influence on the yield of T. kotschyanus and bacterial inoculation of seeds and roots had a greater effect, rather than inoculated separately for organic cultivation.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46554588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Samson Musonerimana, Bez Cristina, G. Habarugira, J. Bigirimana, Venturi Vittorio
{"title":"Characterization of bacterial strains from bacterial culture collection of rice sheath in Burundi highlights an Alcaligenes species strain with antibacterial activity against Pseudomonas fuscovaginae rice pathogen","authors":"Samson Musonerimana, Bez Cristina, G. Habarugira, J. Bigirimana, Venturi Vittorio","doi":"10.5897/ajmr2021.9513","DOIUrl":"https://doi.org/10.5897/ajmr2021.9513","url":null,"abstract":"","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43890993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Audrey, K. Solange, E. A. Eric, A. Emmanuella, M’Bourou Ndombi Fred, Aoussi Serge, Dosso Mireille
{"title":"RAPD-based evaluation revealed genetically diverse populations of Pseudomonas aeruginosa and Staphylococcus aureus lytic bacteriophages isolated in urban sewage and Ebrie Lagoon, Cte dIvoire","authors":"A. Audrey, K. Solange, E. A. Eric, A. Emmanuella, M’Bourou Ndombi Fred, Aoussi Serge, Dosso Mireille","doi":"10.5897/ajmr2021.9547","DOIUrl":"https://doi.org/10.5897/ajmr2021.9547","url":null,"abstract":"","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71092537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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