Zeitschrift fur experimentelle Chirurgie最新文献

Unlabelled: Islets of Langerhans were isolated from the adult wistar rat by means of the collagenase method and cultivated in an incubator at a temperature of 37 degree Celsius and with a mixture of 95% air and 5% CO2. With that each two of the islets were put into every drill hole of multi-dish trays. 1 ml Eagle medium modified by Dulbecco with admixtures of 20% foetal calf serum, 100 microgram/ml Streptomycin and 100 u/ml Penicillin G was the nutrient medium. This medium was exchanged every third day and the released insulin quantity was measured by a radio immunologic test (Phadebas insulin test).

Results: 1. The insulin quantity in the nutrient medium runs to 1097 to 1434 microu/ml two islets if a change took place every third day during an 80 days duration of the culture. 2. The cultivated islets had a demonstrable reactivity of the insulin secretion to glucose and glucagon. 3. Diabetic rats with transplants of nearly 400 cultivated islets recover from diabetic state within one week after transplantation and keep healthy for 8 weeks.

A pancreatic necrosis was produced in 7 piglets by means of a sodiumdesoxycholacid trypsin mixture. 4 animals served as control. All animals get a basis-infusion therapy. Hyperbaric oxygen therapy (HBO) was applied additionally in 3 animals. All control animals survived. The three animals with HBO deceased in average after 22 days, those without HBO deceased already after 4.25 days. Laboratory and circulatory measures show no significant differences. The autopsy statements were different from the age of necrosis. The conclusion by the extension of the average survival time in experimental pancreatic necrosis by means of HBO is that the high lethality of the pancreatic necrosis can be reduced by means of HBO.

The postoperative absorption of the small intestine after selective proximal vagotomy was found out by means of the modified D-xylose absorption test in 23 patients on operating day, and on 1st, 5th, 8th, 9th and, 10th postoperative day. The constant of the adsorption speed was reduced on the operating day and decreased further to the 4th postoperative day. Afterwards the preoperative value was obtained approximately. The temporal state of the relative maximum of the curve of serum concentration (the moment tm) occurred in the same time interval. The tm-value obtained the preoperative value approximately. The dimensions of the absorption in the small intestine was reduced by 36% on the operating day, by 20,5% on the 1st postoperative day, and by 10% on the 4th postoperative day on the arithmetical average. The influence of the gastric emptying after SPV and of gastro-intestinal hormones on the process of absorption was discussed.

Mortality and morbidity of the acute myocardial infarction show an increasing tendency. The therapy, on the other hand, is not much satisfying yet. A myocardial ischemia was produced in 48 minipigs by ligature of the ramus interventricularis anterior of the left coronary artery. Its satellite vein was arterialised by means of plastic shunt. The majority of the animals of the control group without arterialisation did not survive. Electron microscopical controls showed only minimal damages of the ultrastructures in animals after arterialisation of the coronary veins.

Experimental lung transplantations are burdened with a very high rate of complications even is there is a strict consideration of sterile precautions. These complications occur predominantly directly in connection with organ rejection. Extrathoracic complications are rare and predominantly attributed to arterial embolism. All untreated receivers of lung had thoracic complications in autopsy. Only 5% of immune suppressively treated receivers were free of complications. Most frequent complications are pleuropulmonary infections. Weighty complications are haemorrhagic infarcts of the transplant and insufficiency of the bronchus. Only without complications can survived about years the chronic rejection.

The enteric transport kinetics of peroral applied D-xylose was comparatively analysed with help of a modified D-xylose absorption test in man and in experimental pig. The animal experimental investigations were carried out in 10 female hybrid pigs at the age of 3 to 8 months. The absorption quickness and the dimension of the effective small intestine absorption in dependence on the time does not show any statistically significant differences between both biological systems. On the other hand the experimental animals show a statistically secured higher elimination quickness of D-xylose in contrast to the comparison group of healthy adults.

The arterialisation of the coronary veins leads in the acute phase of myocardial ischemia to an effective circulation in the telethermographic picture. The oxygen tension in the tissue increases to 46% of the normal tissue tension in this way. The sinusoids have a nutritive function likely. Although the greatest amount of the blood supplied was drained before becoming metabolised a myocardial necrosis was prevented.

Lesions of the epiphyseal cartilage of the growing bone lead to disturbances of growth. Experiments in the proximal epiphyseal cartilage of the rabbit tibia was carried out as an instruction about the disturbances of growth after Küntscher-nailing. The injured plane of the epiphyseal cartilage amounted to 2%. The disturbances of growth were analysed in dependence on the produced diaphysis fracture and the placement period of the osteosynthetic material. Functionally significant disturbances of growth are not caused after the lesion of the epiphyseal cartilage less than 2% of its plane and removal of the metal after the consolidation of the fracture.

Porcine livers were preserved for 6 hours by simple hypothermic storage using plasma-protein-fraction, albumin, Collins C2, Ringer solution, and Ring-trickle-perfusion. Following glycolytic metabolites were examined: G6P, F6P, FDP, GAP, 2PG, PEP, pyruvate, and lactate. An alteration of glycolytic metabolite-profile by the different preservation methods was not seen. There is a limitation of glycolysis by the pyruvate-kinase-reaction. Glycolysis decreases depending from preservation time. An artificial increasing of glycogen or rate of glycolysis before hepatectomy seems to be without any effect.