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Zeitschrift fur experimentelle Chirurgie, Transplantation, und kunstliche Organe : Organ der Sektion Experimentelle Chirurgie der Gesellschaft fur Chirurgie der DDR最新文献

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[Diagnosis of rejection after pancreatic transplantation in the minipig model]. [微型猪胰腺移植后排斥反应的诊断]。
P Friess, H Lippert, P Müller, F Noack, A Modrejewski, T Benhidjeb, U Hanack, H J Schulz, W D Kranz, M Schöpke

An allogenic transplantation of the total-pancreas with duodenum was carried out in the minipig model. The oral duodenum was closed blindly and the aboral part was led out as a stoma. The portal vein of the transplant was anastomosed end-to-side in the inferior vena cava and the celiac artery of the transplant in the distal aorta. Investigations for early diagnosis of rejection were carried out in this model parallelly respectively one by one. CT/MRT, selective angiography, laboratory chemical parameters were unsuitable for early detection. The pancreatic succus cytology and nuclear medical investigations show reproducible changes caused by rejection before the blood-sugar level was increased.

在小型猪模型上进行了全胰十二指肠同种异体移植。盲闭口十二指肠,将口部引出造口。移植门静脉端侧吻合于下腔静脉,移植腹腔动脉端侧吻合于远端主动脉。排斥反应早期诊断的调查在该模型中依次并行进行。CT/MRT、选择性血管造影、实验室化学参数不适合早期发现。胰浆液细胞学和核医学检查显示,在血糖水平升高之前,排斥反应引起了可重复的变化。
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引用次数: 0
[Effect of the stable prostacyclin analog Iloprost on enzyme release during extracorporeal kidney perfusion]. [稳定的前列环素类似物伊洛前列素对体外肾灌注过程中酶释放的影响]。
J Schabel, H J Hütter, H D Pauer, U Rebmann, H J Forth, H Heynemann, G Mehlhorn

The influence of the stable prostacyclin-analogue Iloprost (Fa. Schering, Berlin) in the enzyme release from extracorporalic perfused kidneys was tested in an experimental investigation. The mechanic hypothermic perfusion was carried out on four different conditions. The estimation of specially cytosolic, lysosomal, and membrane-associated enzymes in the perfusat of the kidneys showed less activities in the Iloprost-protected kidneys. The results after the replantation of the kidneys support the interpretation of the enzyme determinations.

稳定的前列环素类似物依洛前列素(Fa。Schering, Berlin)在体外灌注肾脏的酶释放中进行了实验研究。在四种不同条件下进行机械低温灌注。对肾灌注液中特定的胞质酶、溶酶体酶和膜相关酶的估计显示,在iloprot保护的肾脏中活性较低。肾脏再植后的结果支持酶测定的解释。
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引用次数: 0
[Changes in the motility of the small intestine after autologous and allogenic transplantation in the dog]. [狗自体和异体移植后小肠运动的变化]。
B Luther, M Grzelkowski, C Lehmann

The early and the late motility of the donor organ was measured after 13 allogeneic and 4 autologous small bowel transplantations in mongrel dogs. The postoperative motility indices were increased in contrast to the preoperative values. The activity of allogeneic transplants were greater than these of autologous intestinal segments. The basal tonus of the intestinum obtains a higher level because inhibiting central nervous influences are omitted after the denervation of the mesenteric radix. The contraction frequency remained constant. Disturbances of the motility model with irregular contractions and decrease of the frequency become evident in case of ischemia and rejection of the transplant still before the clinical signs.

测定了13例同种异体和4例自体杂种狗小肠移植后供体器官的早期和晚期运动性。术后的运动指数较术前有所增加。同种异体肠段的移植物活性明显高于自体肠段。肠系膜根去神经支配后,抑制中枢神经的影响被忽略,因此肠基底张力得到更高的水平。收缩频率保持不变。移植物缺血和排斥反应时,运动模型的紊乱表现为不规则收缩和频率降低,比临床症状更早出现。
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引用次数: 0
[Experimental studies of extracellular matrix constituents for coating artificial prostheses before endothelialization]. 人工假体内皮化前涂层细胞外基质成分的实验研究
M Kadletz, K Wolfrum, M Grimm, M Müller, E Wolner

Endothelialization seems to be a possibility to reduce thrombogenicity of artificial vascular prostheses. We have been occupied to find an ideal coating for the cells. Substances of extracellular matrix (ECM) were used: human fibronectin (HFN), laminin, collagen type I + III + IV. We have compared the influence of these materials with regard to adherence, sheer stress resistance and growth of cultivated human vena saphena endothelial cells (SEC). There was not any statistically significant difference according to cell proliferation. During a cultivation period of 7 days SEC increased by population doubling number 5 corresponding to a population doubling time of 76 hours. PTFE prostheses were coated with the materials, seeded with SEC and exposed to sheer stress in a physiological perfusion unit. Adherent SEC were counted after 24 hours of perfusion. Counts were correlated to the number of seeded cells. After perfusion only 23 +/- 4%, resp. 25 +/- 6% of cells were counted on collagen I + IV + HFN and on collagen I + IV + laminin. We found better results on collagen I + III and on collagen I + III + HFN; there were adherent 55 +/- 5%, resp. 53 +/- 7% after perfusion. These results document that there is no difference for SEC cultivation on different native components of basal membrane, under sheer stress conditions there seems to be differences in cell adherence.

内皮化似乎有可能降低人工血管假体的血栓形成性。我们一直在忙着为电池寻找一种理想的涂层。使用细胞外基质(ECM)物质:人纤维连接蛋白(HFN)、层粘连蛋白、I + III + IV型胶原。我们比较了这些材料对培养的人隐静脉内皮细胞(SEC)的粘附性、绝对抗逆性和生长的影响。细胞增殖差异无统计学意义。在7天的培养期间,SEC增加了种群倍增数5,对应于种群倍增时间为76小时。聚四氟乙烯假体涂上材料,用SEC播种,并在生理灌注单元中暴露于绝对应力下。灌注24h后,计数粘附体SEC。计数与种子细胞的数量相关。灌注后仅23 +/- 4%。胶原I + IV + HFN和胶原I + IV +层粘连蛋白占25 +/- 6%。我们发现胶原I + III和胶原I + III + HFN效果更好;贴壁55±5%;53 +/- 7%灌注后。这些结果表明,在不同的天然基膜组分上,SEC培养没有差异,在完全胁迫条件下,细胞粘附似乎存在差异。
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引用次数: 0
[Allogenic cartilage transplantation--immunohistochemical investigations on the antigenicity of the perichondrium]. 同种异体软骨移植——软骨膜抗原性的免疫组织化学研究。
J Bujia, E Wilmer, C Hammer, E Kastenbauer

Perichondrium of human nasal, tracheal, costal and conchal cartilage was analysed by an immunohistological staining technique using monoclonal antibodies against different class II antigens. Our morphological study shows clearly the presence of class II antigens on the perichondrium of nasal, tracheal and conchal cartilage. Therefore the transplantation of cartilage allograft free of perichondrium may be preferred.

用抗不同II类抗原的单克隆抗体对人鼻、气管、肋和耳甲软骨软骨膜进行免疫组织染色。我们的形态学研究清楚地表明II类抗原存在于鼻、气管和耳甲软骨软骨膜上。因此,不含软骨膜的同种异体软骨移植可能是首选。
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引用次数: 0
[Quality assurance in clinical studies: a necessity]. [临床研究的质量保证:必要性]。
M Boll, J Binder, A Siegel, R Grundmann

Good Laboratory Practice (GLP), Good Manufacturing Practice (GMP), and Good Clinical Practice (GCP) are the constituents of a new concept for quality assurance in pharmaceutical research. They are to establish standards for preclinical and experimental research in animals (GLP), for the manufacturing of drugs (GMP), and for trials on medical products in human beings (GCP), respectively. Each of the 3 entities is characterized by the same principles of control; these involve written instruction describing the general operations to be performed, the so called Standard Operating Procedures (SOP), and Quality Assurance Units being responsible for surveying the operations in accordance with the SOP, and ensuring that the generated data will satisfy the requirements for quality. As far as clinical trials are concerned, the SOP is represented by the study protocol and a Monitor provides for the surveying of the trial. GCP is further characterized by ethical principles, which aim primarily at protecting personal integrity and welfare of the trial subjects. The Declaration of Helsinki is the accepted basis for clinical trial ethics. Following the Declaration of Helsinki during the planning, conducting, surveying and evaluating of clinical trials provides for a degree of seriousness being the real hallmark of Good Clinical Practice.

良好实验室规范(GLP)、良好生产规范(GMP)和良好临床规范(GCP)是药物研究质量保证新概念的组成部分。它们将分别为动物临床前和实验研究(GLP)、药物生产(GMP)和医疗产品人体试验(GCP)制定标准。这三个实体都具有相同的控制原则;这些包括描述一般操作的书面说明,所谓的标准操作程序(SOP),以及质量保证单位负责根据SOP调查操作,并确保生成的数据满足质量要求。就临床试验而言,SOP由研究方案代表,监测人员负责对试验进行调查。GCP进一步以伦理原则为特征,其主要目的是保护试验对象的个人诚信和福利。《赫尔辛基宣言》是临床试验伦理的公认基础。根据《赫尔辛基宣言》,在临床试验的规划、实施、调查和评价过程中规定了一定程度的严肃性,这是良好临床实践的真正标志。
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引用次数: 0
[Model and experimental animal studies of the use of hand-controlled catheter-jet-ventilation (CJV) by plastic replacement of the tracheobronchial tree]. [手控导管喷射通气(CJV)通过气管支气管树塑料置换的模型和实验动物研究]。
L Hanf, H Neef, E Csernay, I Trojan, B Vecsei

Pressure and volume flow measurements were performed on a simple model of the tracheobronchial tree using a catheter-jet-ventilation apparatus. This type of respiration is particularly favourable in tracheo-bronchial surgery, i.e. resections and anastomosing, due to the experience of model as well as animal experiments. Under consideration of the particularities of catheter-jet-ventilation, this type of respiration provides excellent conditions for the surgeon. This is valid for the overview of the site of operation and possible manipulation within this region. This method of respiration is suggested as a reliable tool in the hands of less skilled anaesthetists, too, for its easy handling.

使用导管-喷射通气装置对气管-支气管树的简单模型进行压力和体积流量测量。由于模型和动物实验的经验,这种呼吸方式在气管-支气管手术,即切除和吻合中特别有利。考虑到导管喷射通气的特殊性,这种呼吸方式为外科医生提供了良好的条件。这对于手术部位的概述和该区域内可能的操作是有效的。这种呼吸法也被认为是不太熟练的麻醉师手中的可靠工具,因为它易于操作。
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引用次数: 0
[Strengthening of cryodestruction by ultrasonic energy]. [超声能量强化低温破坏]。
W Klug, C Faber, S Busse

The ultrasonic treatment of vital tissue with therapeutically qualified ultrasonic intensity immediate before the cryosurgery leads to a significant enlargement of the plane and the depth of the cryodestruction. This knowledge should be used in the cryosurgical treatment of inoperable carcinomas with extensive tumor mass.

在冷冻手术之前,用治疗合格的超声强度对重要组织进行超声治疗会导致平面和冷冻破坏深度的显着扩大。这方面的知识应用于冷冻手术治疗不能手术的癌与广泛的肿瘤肿块。
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引用次数: 0
[A skin button to anchor and lead out catheters or cable links from the skin]. [从皮肤上固定和引出导管或电缆连接的皮肤按钮]。
C Fritzsch

The manufacture of a skin button for use in chronic animal experiments is described, which is made of silicone rubber and intended to anchor the exit site of intravascular catheters. The button holds the catheter permanently in the skin and can also be used to anchor connections for occlusion cuffs and cables leading to implanted measuring elements.

本文描述了一种用于慢性动物实验的皮肤钮扣的制造,该钮扣由硅橡胶制成,用于锚定血管内导管的出口部位。该按钮将导管永久固定在皮肤中,也可用于固定连接,以连接闭塞袖口和电缆,从而植入测量元件。
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引用次数: 0
[Distance holder for implanted carotid catheters]. [植入式颈动脉导管的距离保持器]。
C Fritzsch
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Zeitschrift fur experimentelle Chirurgie, Transplantation, und kunstliche Organe : Organ der Sektion Experimentelle Chirurgie der Gesellschaft fur Chirurgie der DDR
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