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Zeitschrift fur experimentelle Chirurgie, Transplantation, und kunstliche Organe : Organ der Sektion Experimentelle Chirurgie der Gesellschaft fur Chirurgie der DDR最新文献

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[A technique for pancreatic juice collection in the rat]. [收集大鼠胰液的技术]。
A Königsrainer, C Habringer, R Krausler, G Klima, R Margreiter

Monitoring of exocrine graft function and pancreatic juice cytology have proved to be a reliable method of detection of pancreas allograft rejection. In order to confirm these clinical findings and to define further parameters, experimental work is still needed. The rat was chosen for these experiments and a technique of pancreatic juice procurement developed. In five animals a fully implantable closed reservoir-catheter system was used. For the prevention of bacterial contamination it was necessary to install a mixture of antibiotics and culture medium into the reservoir. Pancreatic juice was procured daily under general anaesthesia. During the observation time of 14 days no further complications were encountered with the exception of one catheter dislocation. The mean amount of juice of 1 cc per day has proved to be sufficient for various enzyme estimations as well as for juice cytology.

监测外分泌功能和胰液细胞学已被证明是检测胰腺异体移植排斥反应的可靠方法。为了证实这些临床发现并确定进一步的参数,仍需要进行实验工作。选用大鼠进行实验,并建立了胰液获取技术。在5只动物中,使用了完全植入式封闭储层导管系统。为了防止细菌污染,有必要在水库中安装抗生素和培养基的混合物。在全身麻醉下每日抽取胰液。在14天的观察中,除1例导管脱位外,无其他并发症发生。每天1毫升果汁的平均量已被证明足以用于各种酶的估计以及果汁细胞学。
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引用次数: 0
[The effect of the oxygen content of the air breathed in on the passive electrical properties of muscle tissue]. [吸入空气的含氧量对肌肉组织被动电特性的影响]。
F Pliquett, V Lap, V Sinz, U Reuter

Due to a want of oxygen in the breathing air there appears a change of the volume of muscle cells, of ion concentrations inside and outside the cells as well as disturbances the structure and membrane potential. These changes are connected with changes of the passive electrical properties of the muscle tissue. Measuring the electrical impedance of the muscle it is possible to diagnose alterations as shock and disorder of microcirculation in an easy way continuously. Investigations with rats show that a decrease of the oxygen content in the inspiration air effects characteristic changes in muscle impedance. These changes are reversible in normoxia.

由于呼吸空气中缺乏氧气,肌肉细胞的体积、细胞内外的离子浓度以及结构和膜电位都会发生变化。这些变化与肌肉组织被动电特性的变化有关。通过测量肌肉的电阻抗,可以方便地连续诊断休克和微循环紊乱等变化。对大鼠的研究表明,吸入空气中氧含量的降低会影响肌肉阻抗的特征变化。这些变化在常氧条件下是可逆的。
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引用次数: 0
[The question of the transmissibility of the results of subcutaneous tests of biomaterials from animals to humans]. [生物材料皮下试验结果从动物向人类传播的问题]。
H Schreiber, F Keller, H P Kinzl, H Hunger, W Knöfler, U Rübling, W Merten

The histologic estimation of the subcutaneous connective-tissue reaction at implants forms an important part of the biocompatibility test. The connective-tissue capsulae around titanium implants in man, guinea-pig, and Wistar-rat were analysed by a special test system because of the question concerning the transmission of results of animal experiments on the man. The subsidence process of the thickness of the connective-tissue capsule and of the cell count per measuring fields were represented in dependence on the time. The findings showed the fundamental similar course of wound healing in man, guinea-pig, and Wistar-rat by the influence of an implant.

植入物皮下结缔组织反应的组织学评估是生物相容性试验的重要组成部分。针对人体动物实验结果的传播问题,对人、豚鼠和wistar大鼠钛植入物周围的结缔组织包膜进行了专门的测试系统分析。以时间为依托,描述了结缔组织囊层厚度和每个测量场细胞数的沉降过程。研究结果显示,受植入物影响,人类、豚鼠和wistar大鼠的伤口愈合过程基本相似。
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引用次数: 0
[Experimental animal studies of the stability of colon anastomoses after supplementary fibrin glue sealing]. [补充纤维蛋白胶密封后结肠吻合口稳定性的实验动物研究]。
R Elfeldt, D Leuze, A Thiede, J Seifert

Sutures of the colon can be insufficient or leaking. This leads in some cases to a peritonitis or sepsis sometimes with lethal outcome. Therefore experiments in animals were performed to investigate the effect of additional applied biogenic glue. Especially in the beginning of the wound healing, at the 4th postoperative day, the firmness could be improved by fibrin glue. The bursting pressure of fibrin glue sealed colon sutures was 94 mmHg, whereas only 66 mmHg was observed in the control group. This additional firmness remains over the whole observation time of 3 weeks. An intensified proliferation of the connective tissue is responsible for this observation which could be substantiated by histological investigations and by measuring the thickness of the scar. If biogenic glue is used in animals it can have the consequence that the recipient reacts with the production of antibodies, since the components of the glue are proteins from different species. Investigations of the serum of animals which had been treated with fibrin glue revealed in a part of them precipitating antibodies against fibrinogen. With regard to this observation a second application of biogenic glue must be done with the necessary precaution.

结肠的缝合线可能不足或漏缝。这在某些情况下会导致腹膜炎或败血症,有时会导致致命的后果。因此,我们进行了动物实验来研究外加生物源胶的效果。特别是在创面愈合初期,术后第4天,纤维蛋白胶可提高创面的硬度。纤维蛋白胶密封结肠缝合线破裂压力为94 mmHg,而对照组仅为66 mmHg。这种额外的坚固性在整个3周的观察时间内保持不变。结缔组织的增殖加剧是造成这一观察结果的原因,这可以通过组织学调查和测量疤痕的厚度来证实。如果在动物身上使用生物胶,可能会导致受体产生抗体,因为这种胶的成分是来自不同物种的蛋白质。对经纤维蛋白胶处理的动物血清的调查显示,部分动物血清中沉淀出抗纤维蛋白原的抗体。关于这一观察,必须采取必要的预防措施进行生物胶的第二次应用。
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引用次数: 0
[The technique of experimental procedures on the floor of the fourth cerebral ventricle of rabbits]. [兔第四脑室底实验程序技术]。
I Sulla, M Ondrejková, M Santa, S Piatniková

The technique of experimental operative procedures in the distal area of the brain stem in the reach of the fourth brain ventricle is described in rabbits. The experiments were carried out in 25 rabbits (breed great-chinchilla, body-weight 4.5 to 5.25 kg) of both sexes. All manipulations were performed in general anesthesia. The best methods for endotracheal intubation of the rabbit and for the surgical technique in the floor of the fourth ventricle were searched.

本文介绍了在兔脑干远端第四脑室处进行实验性手术的技术。实验对象为25只雌雄兔(大栗鼠品种,体重4.5 ~ 5.25 kg)。所有操作均在全麻下进行。探讨了兔气管插管的最佳方法和第四脑室底的手术技术。
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引用次数: 0
[Biocompatibility of various surgical drainage materials in the cytotoxicity and implantation test]. 【各种外科引流材料在细胞毒性和植入试验中的生物相容性】。
J Martin, W Nowak, K D Thiel, P Wutzler, U Kraft

The biocompatibility of 10 materials used for surgical drainage was evaluated in a cytotoxicity test and in rat subcutaneous tissue implantation test. All rubber materials and silikolatex were found to be cytotoxic. There was no correspondence of the results of the cytotoxicity test with those of the implantation test. Therefore various procedures for biocompatibility-testing should be used.

通过细胞毒性试验和大鼠皮下组织植入试验对10种外科引流材料的生物相容性进行了评价。所有的橡胶材料和硅橡胶都被发现有细胞毒性。细胞毒性试验结果与植入试验结果不一致。因此,应使用各种生物相容性试验程序。
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引用次数: 0
[Animal experiments for testing the behavior of compacted bone sections using compression osteosynthesis after hyperthermic denaturation]. [用动物实验测试热变性后压缩成骨的行为]。
R Bocher, R Graul, B Langanke, W Schmidt

In animal experiments on domestic pigs the behaviour of hyperthermically denatured autogenic compact bone grafts after orthotopic replantation was investigated histologically. It was shown that the inductive osteogenetic celldifferentiation of the lair tissue caused by the autogenetic osteoimplantation depends on the location relationship of the replanted bone grafts to the lair bone tissue. This differentiation had progressed after 14 and 21 days further in the upper indifference area than in the contact and fissure healing area.

在动物实验中,对家猪热变性的自体致密骨移植物在原位再植后的行为进行了组织学研究。结果表明,自体骨植入诱导的成骨细胞分化取决于移植物与老窝骨组织的位置关系。这种分化在14天和21天后在上无差别区比在接触区和裂隙愈合区进展得更快。
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引用次数: 0
[Immunoglobulin therapy of postoperative sepsis]. 术后败血症的免疫球蛋白治疗。
C Wesoly, N Kipping, R Grundmann

35 patients with septic postoperative complications entered a prospectively randomized study. The clinical state of these patients was daily determined using the sepsis score described by Elebute and Stoner. Endotoxin and antithrombin III were measured in the plasma using the limulus-amoebocyte-lysat test for endotoxin determination. The septic patients were treated with an immunoglobulin preparation (Pentaglobin) administered by the intravenous route. This preparation is enriched in IgM and IgA. Due to the immunoglobulin therapy the endotoxin titres decreased; simultaneously a reduction of mortality and shortening of time of hospitalization and of mechanical ventilation were observed.

35例脓毒性术后并发症患者进入前瞻性随机研究。每日使用Elebute和Stoner描述的脓毒症评分来确定这些患者的临床状态。采用鲎试剂-溶阿米巴细胞法测定血浆内毒素和抗凝血酶III。脓毒症患者经静脉注射免疫球蛋白制剂(五血红蛋白)治疗。该制剂富含IgM和IgA。免疫球蛋白治疗使内毒素滴度降低;同时观察到死亡率降低,住院时间和机械通气时间缩短。
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引用次数: 0
[Effect of various fixation agents on ultrasound damping in normal and pathological testicular tissues]. [不同固定剂对正常和病理睾丸组织超声阻尼的影响]。
H Heynemann, K P Richter, J Haerting, H J Holzhausen, J Baumann, K Jenderka, R Millner, B Langkopf

In normal and pathological testicular tissue the influence of various fixing agents (Formalin solution, Bouins solution, Hellys solution, Carnoys solution) concerning the acoustical properties of testicular tissue was examined. By means of an ultrasound spectroscopic measurement technique the ultrasound damping was registered by calculation of the ultrasound damping coefficient alpha. The results show that the most lower alpha values were obtained by fixation with formalin solution as well in normal as in altered testicular tissue. The results of fixation according to Helly, Carnoy and Bouin did show markedly differences. Therefore, the fixation of testicular tissue using formalin solution is the best way for experimental examinations of the ultrasound damping behaviour.

在正常和病理睾丸组织中,研究了各种固定剂(福尔马林溶液、博因溶液、赫利斯溶液、卡诺伊斯溶液)对睾丸组织声学特性的影响。利用超声光谱测量技术,通过计算超声阻尼系数来记录超声阻尼。结果表明,在正常和改变的睾丸组织中,福尔马林溶液固定得到的α值最低。根据Helly, Carnoy和Bouin的固定结果确实显示出显着差异。因此,用福尔马林溶液固定睾丸组织是超声阻尼性能实验检查的最佳方法。
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引用次数: 0
[New organ-preserving technics for third-grade splenic trauma. An experimental study]. 三级脾损伤保器官新技术。一项实验研究]。
S Uranüs, L Kronberger, A Beham, K Neumayer, W Kröll, D Aktuna

We studied two new techniques for orthotopic preservation of the spleen in an animal model with respect to their technical feasibility and their hemostatic efficacy. This study was performed in two stages. In the first, the treatment of central bursting ruptures by splenorrhaphy using a resorbable compressive mesh was tested. 20 sheep have been used for this part of the study. They were divided into four groups (A: control, B: sham operation, C: splenectomy, D: mesh splenorrhaphy). Following completion of this stage, two animals from group A and two from group B were used to test a second experimental setting. Experimental trauma corresponding to isolated third degree polar rupture of the spleen was induced. This was then treated by a polar resection using stapling instruments. All animals from each stage were subjected to spleen scanning three months postoperatively, followed by splenectomy. The spleens were then examined histologically. Both macroscopically and microscopically normal splenic tissue was found. No difference between the different groups could be detected by laboratory investigations. Gamma-globulins and total serum proteins were within normal ranges after five weeks. Splenic scan revealed an excellent function of the pulp. Both techniques are distinguished by their facility and rapidity, as well as reliable hemostasis and minimal technical prerequisites.

我们在动物模型上研究了两种原位保存脾脏的新技术的技术可行性和止血效果。本研究分两个阶段进行。首先,通过使用可吸收压缩网进行脾脏修补治疗中央破裂进行了测试。这部分研究使用了20只羊。患者分为4组(A:对照组、B:假手术组、C:脾切除术组、D:网状脾切除术组)。在这一阶段完成后,A组和B组的两只动物被用于第二个实验环境的测试。实验创伤对应于离体脾三度极性破裂。然后用吻合器进行极性切除治疗。每个阶段的所有动物术后3个月进行脾脏扫描,然后进行脾切除术。然后对脾脏进行组织学检查。肉眼和显微镜下均见脾脏组织正常。实验室调查未发现不同组之间的差异。5周后γ -球蛋白和血清总蛋白均在正常范围内。脾扫描显示牙髓功能良好。这两种技术的特点是它们的便利和快速,以及可靠的止血和最低的技术要求。
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Zeitschrift fur experimentelle Chirurgie, Transplantation, und kunstliche Organe : Organ der Sektion Experimentelle Chirurgie der Gesellschaft fur Chirurgie der DDR
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