Abnormal uterine bleeding (AUB) is the most common gynecological problem and is often accompanied with uterine fibroid. Very limited options are available in biomedicine for the AUB. In this reported case, AUB associated with stasis dermatitis was managed on the line of treatment of Vataja Yonivyapada with satisfactory results. This is a case of AUB in uterine fibroid associated with stasis dermatitis in the left lower limb of a 39-year-old female. Gokshuradi Guggulu 750 mg, Shatavari Churna - 2 g, Gairika - 500 mg, and Mukta Shukti Pishti - 500 mg were given twice a day for 18 months, Pushyanuga Churna - 2 g, Chandraprabha Vati - 250 mg, and Arsha Kuthara Rasa 250 mg twice a day for 8 months, and Gokshura churna - 2 g twice a day for 14 months were given to her as oral medication. Jatyadi taila was applied locally twice a day on affected area for 17 months. Four sets of Anuvasana Basti (medicated oil enema), one set of Raktamokshana (therapeutic bloodletting) with Alabu, and three sets of Raktamokshana with Shringa were also adopted in 14 months of treatment duration. After 14 months of treatment, the size of the bulky uterus was reduced from 108 mm × 76 mm × 70 mm in May 2018 to 102 mm × 64 mm × 55 mm in May 2019 and from 7.0 to 6.6 mm in endometrium thickness. The fibroid was resolved, and abnormal bleeding was arrested. This case demonstrates that Ayurveda oral medication and Panchakarma based on the management of Vataja Yonivyapada may be useful in AUB associated with stasis dermatitis.
{"title":"Ayurvedic Management of Abnormal Uterine Bleeding in Uterine Fibroid","authors":"Kshipra Rajoria, S. Singh","doi":"10.4103/asl.asl_72_22","DOIUrl":"https://doi.org/10.4103/asl.asl_72_22","url":null,"abstract":"\u0000 Abnormal uterine bleeding (AUB) is the most common gynecological problem and is often accompanied with uterine fibroid. Very limited options are available in biomedicine for the AUB. In this reported case, AUB associated with stasis dermatitis was managed on the line of treatment of Vataja Yonivyapada with satisfactory results. This is a case of AUB in uterine fibroid associated with stasis dermatitis in the left lower limb of a 39-year-old female. Gokshuradi Guggulu 750 mg, Shatavari Churna - 2 g, Gairika - 500 mg, and Mukta Shukti Pishti - 500 mg were given twice a day for 18 months, Pushyanuga Churna - 2 g, Chandraprabha Vati - 250 mg, and Arsha Kuthara Rasa 250 mg twice a day for 8 months, and Gokshura churna - 2 g twice a day for 14 months were given to her as oral medication. Jatyadi taila was applied locally twice a day on affected area for 17 months. Four sets of Anuvasana Basti (medicated oil enema), one set of Raktamokshana (therapeutic bloodletting) with Alabu, and three sets of Raktamokshana with Shringa were also adopted in 14 months of treatment duration. After 14 months of treatment, the size of the bulky uterus was reduced from 108 mm × 76 mm × 70 mm in May 2018 to 102 mm × 64 mm × 55 mm in May 2019 and from 7.0 to 6.6 mm in endometrium thickness. The fibroid was resolved, and abnormal bleeding was arrested. This case demonstrates that Ayurveda oral medication and Panchakarma based on the management of Vataja Yonivyapada may be useful in AUB associated with stasis dermatitis.","PeriodicalId":7805,"journal":{"name":"Ancient Science of Life","volume":"120 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139383238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Coconut shell is an inexpensive, easily available, and usually discarded material from the plant source Cocos nucifera Linn. belonging to the Arecaceae family. The internal administration of water boiled with endocarp of coconut fruit (coconut shell) is used as a folklore remedy in Kerala for treating metabolic disorders. This folklore claim has to be scientifically validated by comprehensive chemical profiling of coconut (Cocos nucifera Linn.) shell. The present study was designed to conduct a systematic screening of coconut shell for identification of its bioactive secondary metabolites and validation of its analogous pharmacotherapeutic potentials. The present research work is focused on the screening of dried ripe coconut (Cocos nucifera Linn.) shell for its bioactive secondary metabolites based on microscopic, histochemical, and phytochemical studies. Characteristic presence of thin-walled fibers and fibrosclereids were observed in the powder microscopy of coconut shell. Histochemical studies were suggestive of the presence of lignified cells and tannins in the test drug. Further phytochemical studies of Cocos nucifera Linn. ascertained that the endocarp is abounded with diverse bioactive phytoconstituents like flavonoids, alkaloids, phenols, steroids, tannins, and saponins which possess varied antioxidant, anti-inflammatory, anti-obesity, and antidiabetic activities. The study outcome suggests coconut shell as a novel natural source of various bioactive secondary metabolites and these findings can be integrated for further researches exploring the comprehensive pharmacotherapeutic prospective of the test drug.
{"title":"Screening of Coconut (Cocos nucifera Linn.) Shell for its Bioactive Secondary Metabolites and Analogous Pharmacotherapeutic Prospective","authors":"V. Sreekala, Kamal Nayan Dwivedi, R. Rajesh","doi":"10.4103/asl.asl_119_21","DOIUrl":"https://doi.org/10.4103/asl.asl_119_21","url":null,"abstract":"\u0000 \u0000 \u0000 Coconut shell is an inexpensive, easily available, and usually discarded material from the plant source Cocos nucifera Linn. belonging to the Arecaceae family. The internal administration of water boiled with endocarp of coconut fruit (coconut shell) is used as a folklore remedy in Kerala for treating metabolic disorders. This folklore claim has to be scientifically validated by comprehensive chemical profiling of coconut (Cocos nucifera Linn.) shell.\u0000 \u0000 \u0000 \u0000 The present study was designed to conduct a systematic screening of coconut shell for identification of its bioactive secondary metabolites and validation of its analogous pharmacotherapeutic potentials.\u0000 \u0000 \u0000 \u0000 The present research work is focused on the screening of dried ripe coconut (Cocos nucifera Linn.) shell for its bioactive secondary metabolites based on microscopic, histochemical, and phytochemical studies.\u0000 \u0000 \u0000 \u0000 Characteristic presence of thin-walled fibers and fibrosclereids were observed in the powder microscopy of coconut shell. Histochemical studies were suggestive of the presence of lignified cells and tannins in the test drug. Further phytochemical studies of Cocos nucifera Linn. ascertained that the endocarp is abounded with diverse bioactive phytoconstituents like flavonoids, alkaloids, phenols, steroids, tannins, and saponins which possess varied antioxidant, anti-inflammatory, anti-obesity, and antidiabetic activities.\u0000 \u0000 \u0000 \u0000 The study outcome suggests coconut shell as a novel natural source of various bioactive secondary metabolites and these findings can be integrated for further researches exploring the comprehensive pharmacotherapeutic prospective of the test drug.\u0000","PeriodicalId":7805,"journal":{"name":"Ancient Science of Life","volume":"24 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139383345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Kumudhaveni, Ramalingam Radha, Margesan Thirumal, Rajkishore Vijayabharathi
Plants are extremely useful to humankind as medicine, and hence the properties of medicinal plants have to be scientifically evaluated. The aim of this study is to establish pharmacognostical and phytochemical parameters for stem bark of Ximenia americana Linn. (Olacaceae). Macroscopical, microscopical evaluation, powder microscopical studies, quantitative microscopy, physicochemical evaluation, fluorescence analysis, analysis of inorganic elements, and determination of heavy metal contamination were performed as per the World Health Organization guidelines and standard methods. To evaluate various phytoconstituents, phytochemical investigations such as preliminary phytochemical screening, high-performance thin-layer chromatography, and high-performance liquid chromatography were performed. Microscopical studies showed the presence of thick cortex, periderm cells, phloem parenchyma, phloem fibers, sieve elements, prismatic and granular calcium oxalate crystals, and resinous cells. The length and width of fibers, stone cells, and diameter of starch grains were measured. Physicochemical evaluation like ash values has shown total ash – 8.01% ± 0.02% w/w, acid-insoluble ash – 1.53% ± 0.02% w/w, water-soluble ash – 1.54% ± 0.01% w/w, sulfated ash – 12.02% ± 0.07% w/w, water-soluble hot extraction – 26.48 ± 0.02% w/w, cold maceration – 11.76% ± 0.04% w/w, alcohol soluble – 15.69% ± 0.03%w/w, ether soluble – 2.06% ± 0.03% w/w, and loss on drying – 4.88% ± 0.01% w/w. Pharmacognostical study on the stem bark of Ximenia americana may be reported as first. The results of pharmacognostical and phytochemical studies will be helpful for authentication and provide quality control parameters for the stem bark of this plant.
{"title":"Evaluation of Pharmacognostical and Phytochemical Studies for the Development of Quality Control Parameters for Stem Bark of Ximenia americana Linn. (Olacaceae)","authors":"B. Kumudhaveni, Ramalingam Radha, Margesan Thirumal, Rajkishore Vijayabharathi","doi":"10.4103/asl.asl_7_22","DOIUrl":"https://doi.org/10.4103/asl.asl_7_22","url":null,"abstract":"\u0000 \u0000 \u0000 Plants are extremely useful to humankind as medicine, and hence the properties of medicinal plants have to be scientifically evaluated.\u0000 \u0000 \u0000 \u0000 The aim of this study is to establish pharmacognostical and phytochemical parameters for stem bark of Ximenia americana Linn. (Olacaceae).\u0000 \u0000 \u0000 \u0000 Macroscopical, microscopical evaluation, powder microscopical studies, quantitative microscopy, physicochemical evaluation, fluorescence analysis, analysis of inorganic elements, and determination of heavy metal contamination were performed as per the World Health Organization guidelines and standard methods. To evaluate various phytoconstituents, phytochemical investigations such as preliminary phytochemical screening, high-performance thin-layer chromatography, and high-performance liquid chromatography were performed.\u0000 \u0000 \u0000 \u0000 Microscopical studies showed the presence of thick cortex, periderm cells, phloem parenchyma, phloem fibers, sieve elements, prismatic and granular calcium oxalate crystals, and resinous cells. The length and width of fibers, stone cells, and diameter of starch grains were measured. Physicochemical evaluation like ash values has shown total ash – 8.01% ± 0.02% w/w, acid-insoluble ash – 1.53% ± 0.02% w/w, water-soluble ash – 1.54% ± 0.01% w/w, sulfated ash – 12.02% ± 0.07% w/w, water-soluble hot extraction – 26.48 ± 0.02% w/w, cold maceration – 11.76% ± 0.04% w/w, alcohol soluble – 15.69% ± 0.03%w/w, ether soluble – 2.06% ± 0.03% w/w, and loss on drying – 4.88% ± 0.01% w/w.\u0000 \u0000 \u0000 \u0000 Pharmacognostical study on the stem bark of Ximenia americana may be reported as first. The results of pharmacognostical and phytochemical studies will be helpful for authentication and provide quality control parameters for the stem bark of this plant.\u0000","PeriodicalId":7805,"journal":{"name":"Ancient Science of Life","volume":"28 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139383676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Medicinal plants have been used in traditional medical practices since prehistoric times. It is popularly known that roots, rhizomes, and leaves of some Smilax species are widely used for treating various diseases such as toothache, muscular pain, urinary diseases, and jaundice. This review aims to document the information regarding major phytochemicals and pharmacological activity of selected Smilax species in India. Details of different species of Smilax species in India were collected from online databases such as https://wcsp.science.kew.org/(2022), https://powo.science.kew.org (2022), and https://www.ipni.org (2022). All the available information related to phytochemistry and pharmacological activities was collected from electronic databases such as Academic Journals, Springer, PubMed, Google Scholar, ScienceDirect, MEDLINE, Scopus, Web of Science, and other primary and secondary sources. Literature survey revealed that members of the genus Smilax possess large quantities of steroids and phenolic compounds and possess various bioactive properties such as antibacterial, antifungal, antioxidant, and analgesic activities. This review concludes that more research on the genus Smilax is required to identify and evaluate the presence of potential bioactive constituents, and further investigations may be led to the development of new drugs.
{"title":"Phytochemistry and Pharmacology of Selected Species of Smilax L. (Smilacaceae) in India","authors":"Neethu Uthaman, Valukattil Ponnachan Thomas, BinoyT Thomas.","doi":"10.4103/asl.asl_76_22","DOIUrl":"https://doi.org/10.4103/asl.asl_76_22","url":null,"abstract":"\u0000 \u0000 \u0000 Medicinal plants have been used in traditional medical practices since prehistoric times. It is popularly known that roots, rhizomes, and leaves of some Smilax species are widely used for treating various diseases such as toothache, muscular pain, urinary diseases, and jaundice.\u0000 \u0000 \u0000 \u0000 This review aims to document the information regarding major phytochemicals and pharmacological activity of selected Smilax species in India. Details of different species of Smilax species in India were collected from online databases such as https://wcsp.science.kew.org/(2022), https://powo.science.kew.org (2022), and https://www.ipni.org (2022). All the available information related to phytochemistry and pharmacological activities was collected from electronic databases such as Academic Journals, Springer, PubMed, Google Scholar, ScienceDirect, MEDLINE, Scopus, Web of Science, and other primary and secondary sources.\u0000 \u0000 \u0000 \u0000 Literature survey revealed that members of the genus Smilax possess large quantities of steroids and phenolic compounds and possess various bioactive properties such as antibacterial, antifungal, antioxidant, and analgesic activities.\u0000 \u0000 \u0000 \u0000 This review concludes that more research on the genus Smilax is required to identify and evaluate the presence of potential bioactive constituents, and further investigations may be led to the development of new drugs.\u0000","PeriodicalId":7805,"journal":{"name":"Ancient Science of Life","volume":"6 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139381165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Kaur, G. Ruknuddin, M. Nariya, B. Patgiri, P. Bedarkar, P. Prajapati
Background: Steroidal and non-steroidal anti-inflammatory agents have several limitations, due to which people are moving towards use of traditional medicines. Considering this, the current attempt is aimed to evaluate in vivo anti-inflammatory effect of a poly-herbal formulation Śirīṣāvaleha prepared in presence of water and Kanji (sour gruel). Kanji is anticipated to bring extraction of more principles from the raw drugs and may improve therapeutic attributes of the drug. Considering this, Kanji was used as a liquid medium for preparation of Śirīṣāvaleha and was evaluated for anti-inflammatory activity in comparison to Śirīṣāvaleha prepared with water. Materials and Methods: The activity was screened in carrageenan induced rat paw oedema model in albino rats. The raw materials were collected; authenticated and trial formulations were prepared by following standard classical guidelines. Randomly selected animals were divided into four groups of six animals each. The test drugs were administered orally at a dose of 1.8 g/kg for five days. Phenylbutazone was used as standard anti-inflammatory drug for comparison. Results: Results showed the presence of pronounced anti-inflammatory activity in Śirīṣāvaleha prepared with water (SW) followed by Śirīṣāvaleha prepared with Kanji (SK). Conclusion: Study shows that both samples of Śirīṣāvaleha have anti-inflammatory effect.
{"title":"Anti-inflammatory effect of Śirīṣāvaleha prepared by two liquid media on carrageenan induced rat paw oedema model","authors":"H. Kaur, G. Ruknuddin, M. Nariya, B. Patgiri, P. Bedarkar, P. Prajapati","doi":"10.4103/asl.ASL_11_18","DOIUrl":"https://doi.org/10.4103/asl.ASL_11_18","url":null,"abstract":"Background: Steroidal and non-steroidal anti-inflammatory agents have several limitations, due to which people are moving towards use of traditional medicines. Considering this, the current attempt is aimed to evaluate in vivo anti-inflammatory effect of a poly-herbal formulation Śirīṣāvaleha prepared in presence of water and Kanji (sour gruel). Kanji is anticipated to bring extraction of more principles from the raw drugs and may improve therapeutic attributes of the drug. Considering this, Kanji was used as a liquid medium for preparation of Śirīṣāvaleha and was evaluated for anti-inflammatory activity in comparison to Śirīṣāvaleha prepared with water. Materials and Methods: The activity was screened in carrageenan induced rat paw oedema model in albino rats. The raw materials were collected; authenticated and trial formulations were prepared by following standard classical guidelines. Randomly selected animals were divided into four groups of six animals each. The test drugs were administered orally at a dose of 1.8 g/kg for five days. Phenylbutazone was used as standard anti-inflammatory drug for comparison. Results: Results showed the presence of pronounced anti-inflammatory activity in Śirīṣāvaleha prepared with water (SW) followed by Śirīṣāvaleha prepared with Kanji (SK). Conclusion: Study shows that both samples of Śirīṣāvaleha have anti-inflammatory effect.","PeriodicalId":7805,"journal":{"name":"Ancient Science of Life","volume":"37 1","pages":"81 - 85"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70684459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Bhatt, A. Dey, Satyajyoti Kanjilal, H. Srikanth, R. Biswas, P. Chakraborty, D. Gandhi, A. Narwaria, C. Katiyar
Background: In Ayurveda, use of multiple herbs in a single formulation is popular and polyherbal Ayurvedic formulations are widely used as growth promoters, gastrointestinal and hepatic regulators, hepatic tonics and so on. Despite the widespread use, there is a lack of scientific evidence on their efficacy and safety. Aim: This study was undertaken to validate the efficacy by evaluating the hepatoprotective activity of one such polyherbal blend (LIVT) on HepG2 cell line. Materials and Methods: Four doses of test formulation (62.5, 125, 250 and 500 μg/ml) were tested on D-galactosamine induced HepG2 cell toxicity model. MTT assay was performed to determine the dose range for the hepatoprotective study. Results: The test formulation exhibited significant (P < 0.05) cytoprotective activity with a maximum protection of 37% at dose 62.5 μg/ml, and the activity was comparable with that of the standard, silymarin. Conclusion: The results of the present study indicate that the polyherbal blend demonstrated a significant hepatoprotective activity and could be used as an active herbal alternative for the treatment of liver ailments.
{"title":"In vitro hepatoprotective activity of a polyherbal formulation on HepG2 cell line","authors":"B. Bhatt, A. Dey, Satyajyoti Kanjilal, H. Srikanth, R. Biswas, P. Chakraborty, D. Gandhi, A. Narwaria, C. Katiyar","doi":"10.4103/asl.ASL_57_18","DOIUrl":"https://doi.org/10.4103/asl.ASL_57_18","url":null,"abstract":"Background: In Ayurveda, use of multiple herbs in a single formulation is popular and polyherbal Ayurvedic formulations are widely used as growth promoters, gastrointestinal and hepatic regulators, hepatic tonics and so on. Despite the widespread use, there is a lack of scientific evidence on their efficacy and safety. Aim: This study was undertaken to validate the efficacy by evaluating the hepatoprotective activity of one such polyherbal blend (LIVT) on HepG2 cell line. Materials and Methods: Four doses of test formulation (62.5, 125, 250 and 500 μg/ml) were tested on D-galactosamine induced HepG2 cell toxicity model. MTT assay was performed to determine the dose range for the hepatoprotective study. Results: The test formulation exhibited significant (P < 0.05) cytoprotective activity with a maximum protection of 37% at dose 62.5 μg/ml, and the activity was comparable with that of the standard, silymarin. Conclusion: The results of the present study indicate that the polyherbal blend demonstrated a significant hepatoprotective activity and could be used as an active herbal alternative for the treatment of liver ailments.","PeriodicalId":7805,"journal":{"name":"Ancient Science of Life","volume":"35 1","pages":"99 - 101"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70685012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Srivastava, Vikas Sharma, S. Verma, R. Singh, A. Jadhav
Background: Medasakha (Litsea chinensis) is a medium sized tree, heartwood of which is extensively used in Ayurveda for the treatment of various diseases. Count of this species is declining in the wild and in future this species may be difficult to obtain for use in Ayurveda and other traditional systems of medicine. It is exploited mainly for its medicinal heartwood. Hence the use of alternative parts of this plant in place of its heartwood would be beneficial for its survival. Objective: Present study is carried out on L. chinensis to phytochemically evaluate the possibilities of using its small branches as a substitute to its heartwood. Materials and Methods: Physicochemical parameters and preliminary phytochemical screening were carried out using standard methods. Total phenolic and total flavonoid contents were estimated spectrophotometrically using Folin-ciocalteu and aluminum chloride method, respectively. CAMAG HPTLC system equipped with semi-automatic applicator was used for HPTLC profiling. n-Hexane, ethyl acetate and ethanol extracts of heartwood and small braches were developed in suitable mobile phase using standard procedures and visualized in UV 254, 366 nm and in white light after derivatization with anisaldehyde-sulphuric acid reagent. Results: Phytochemical analysis and HPTLC profile of different extracts of heartwood and small branches showed the presence of almost similar phytochemicals in both the parts of this plant which suggests that small branches may be used in place of heartwood and vice-versa after comparison and confirmation of same for pharmacological activities. Conclusion: This study provides the base for further study to use small branches of L. chinensis as a substitute to its heartwood.
{"title":"Plant part substitution in Litsea Chinensis for medicinal use: A comparative phytochemical approach","authors":"B. Srivastava, Vikas Sharma, S. Verma, R. Singh, A. Jadhav","doi":"10.4103/asl.ASL_168_16","DOIUrl":"https://doi.org/10.4103/asl.ASL_168_16","url":null,"abstract":"Background: Medasakha (Litsea chinensis) is a medium sized tree, heartwood of which is extensively used in Ayurveda for the treatment of various diseases. Count of this species is declining in the wild and in future this species may be difficult to obtain for use in Ayurveda and other traditional systems of medicine. It is exploited mainly for its medicinal heartwood. Hence the use of alternative parts of this plant in place of its heartwood would be beneficial for its survival. Objective: Present study is carried out on L. chinensis to phytochemically evaluate the possibilities of using its small branches as a substitute to its heartwood. Materials and Methods: Physicochemical parameters and preliminary phytochemical screening were carried out using standard methods. Total phenolic and total flavonoid contents were estimated spectrophotometrically using Folin-ciocalteu and aluminum chloride method, respectively. CAMAG HPTLC system equipped with semi-automatic applicator was used for HPTLC profiling. n-Hexane, ethyl acetate and ethanol extracts of heartwood and small braches were developed in suitable mobile phase using standard procedures and visualized in UV 254, 366 nm and in white light after derivatization with anisaldehyde-sulphuric acid reagent. Results: Phytochemical analysis and HPTLC profile of different extracts of heartwood and small branches showed the presence of almost similar phytochemicals in both the parts of this plant which suggests that small branches may be used in place of heartwood and vice-versa after comparison and confirmation of same for pharmacological activities. Conclusion: This study provides the base for further study to use small branches of L. chinensis as a substitute to its heartwood.","PeriodicalId":7805,"journal":{"name":"Ancient Science of Life","volume":"37 1","pages":"57 - 62"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70684291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Kṣāra Kalpanā (Ayurvedic alkali formulation) mainly deals with extraction of alkaline material from herb, animals and minerals. Kṣāra preparation mainly has variation in the concentration of Water used for extraction. Objective: To assess the effect of variation in concentration of water and plant part used on the physicochemical parameters of Yavakṣāra (YK). Materials and Methods: YK was prepared by using whole plant with seeds and without seeds. Further these batches were divided depend upon of 4, 6 and 8 times of concentration of water used for extraction. YK was analyzed by using the XRD, AAS-ICP and FTIR. Results: All samples contain Halite crystals of NaCl and Sylvine crystals of KCl. Halite and Sylvine crystals generally formed due to evaporation which is main step in the preparation of Kṣāra Kalpanā. Crystal size of NaCl and KCl is near to nanoparticles (in the range between 90 to 135 nm). YK contains other elements like Fe, Mg and Ca in small amount with slight variance in quantity in different batches. FTIR shows presence of same functional group (C-H of (Alkyl) amines) in all samples of YK. Absences (Pb, As) or traces (Hg) of heavy metal indicate care taken during harvesting and manufacturing. Conclusion: Slight variation is seen in the elements, their concentration and functional group in different batches of YK. Variation in the concentration of water alters the frequency of filtration and affect the period of sedimentation. A batch prepared with less concentration of water (4 times) and without seeds seems to be cost effective.
{"title":"Effect of variation in concentration of water and plant part used on physicochemical properties of Yavakṣāra","authors":"Akash Kawasthe, P. Gandhi","doi":"10.4103/asl.ASL_58_18","DOIUrl":"https://doi.org/10.4103/asl.ASL_58_18","url":null,"abstract":"Introduction: Kṣāra Kalpanā (Ayurvedic alkali formulation) mainly deals with extraction of alkaline material from herb, animals and minerals. Kṣāra preparation mainly has variation in the concentration of Water used for extraction. Objective: To assess the effect of variation in concentration of water and plant part used on the physicochemical parameters of Yavakṣāra (YK). Materials and Methods: YK was prepared by using whole plant with seeds and without seeds. Further these batches were divided depend upon of 4, 6 and 8 times of concentration of water used for extraction. YK was analyzed by using the XRD, AAS-ICP and FTIR. Results: All samples contain Halite crystals of NaCl and Sylvine crystals of KCl. Halite and Sylvine crystals generally formed due to evaporation which is main step in the preparation of Kṣāra Kalpanā. Crystal size of NaCl and KCl is near to nanoparticles (in the range between 90 to 135 nm). YK contains other elements like Fe, Mg and Ca in small amount with slight variance in quantity in different batches. FTIR shows presence of same functional group (C-H of (Alkyl) amines) in all samples of YK. Absences (Pb, As) or traces (Hg) of heavy metal indicate care taken during harvesting and manufacturing. Conclusion: Slight variation is seen in the elements, their concentration and functional group in different batches of YK. Variation in the concentration of water alters the frequency of filtration and affect the period of sedimentation. A batch prepared with less concentration of water (4 times) and without seeds seems to be cost effective.","PeriodicalId":7805,"journal":{"name":"Ancient Science of Life","volume":"370 1","pages":"94 - 98"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70685025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Book review: An introduction to ayurveda","authors":"A. Shukla, A. Shukla","doi":"10.4103/ASL.ASL_166_17","DOIUrl":"https://doi.org/10.4103/ASL.ASL_166_17","url":null,"abstract":"","PeriodicalId":7805,"journal":{"name":"Ancient Science of Life","volume":"37 1","pages":"108 - 110"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70684194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: The present study was undertaken to evaluate the potential role of ethanol extract of seeds of Lens culinaris in attenuation of Cisplatin-induced nephrotoxicity in male Albino rats. Materials and Methods: Seeds of Lens culinaris were powdered, defatted and ethanol extract was prepared by hot extraction method. The ethanol extract so prepared was subjected to preliminary phytochemical evaluation and fingerprint analysis using high performance thin layer chromatography. Nephroprotective potential of extract was evaluated at 200 and 400 mg/kg b.w. in both curative and prophylactic regimens in male Albino rats. Nephrotoxicity was induced by single intra peritoneal injection of Cisplatin at a dose of 5 mg/kg b.w. Nephroprotective activity was assessed by determining serum markers, urinary parameters, lipid peroxidation and antioxidant levels in renal tissue. Results: Cisplatin had induced marked acute renal toxicity as manifested by a significant increase in serum marker levels, urinary total protein, lipid peroxidation and decrease in urinary creatinine, catalase (CAT), superoxide dismutase (SOD), and reduced glutathione (GSH). The administration of extract significantly attenuated the Cisplatin-induced nephrotoxicity remarkably by restoring the biochemical and oxidative stress markers in both curative and prophylactic regimens in a dose dependent manner. Histological and immunohistochemical studies also substantiated the biochemical studies. Conclusion: The findings of the present study provides corroborative scientific evidence for the attenuation of Cisplatin-induced nephrotoxicity by seeds of Lens culinaris and validates the ethno-medicinal use of this seeds as renoprotective agent.
{"title":"Attenuation of cisplatin-induced nephrotoxicity by ethanol extract of seeds of Lens culinaris medik","authors":"A. Sreedevi, Kaveripakam Saisruthi","doi":"10.4103/asl.ASL_173_17","DOIUrl":"https://doi.org/10.4103/asl.ASL_173_17","url":null,"abstract":"Aim: The present study was undertaken to evaluate the potential role of ethanol extract of seeds of Lens culinaris in attenuation of Cisplatin-induced nephrotoxicity in male Albino rats. Materials and Methods: Seeds of Lens culinaris were powdered, defatted and ethanol extract was prepared by hot extraction method. The ethanol extract so prepared was subjected to preliminary phytochemical evaluation and fingerprint analysis using high performance thin layer chromatography. Nephroprotective potential of extract was evaluated at 200 and 400 mg/kg b.w. in both curative and prophylactic regimens in male Albino rats. Nephrotoxicity was induced by single intra peritoneal injection of Cisplatin at a dose of 5 mg/kg b.w. Nephroprotective activity was assessed by determining serum markers, urinary parameters, lipid peroxidation and antioxidant levels in renal tissue. Results: Cisplatin had induced marked acute renal toxicity as manifested by a significant increase in serum marker levels, urinary total protein, lipid peroxidation and decrease in urinary creatinine, catalase (CAT), superoxide dismutase (SOD), and reduced glutathione (GSH). The administration of extract significantly attenuated the Cisplatin-induced nephrotoxicity remarkably by restoring the biochemical and oxidative stress markers in both curative and prophylactic regimens in a dose dependent manner. Histological and immunohistochemical studies also substantiated the biochemical studies. Conclusion: The findings of the present study provides corroborative scientific evidence for the attenuation of Cisplatin-induced nephrotoxicity by seeds of Lens culinaris and validates the ethno-medicinal use of this seeds as renoprotective agent.","PeriodicalId":7805,"journal":{"name":"Ancient Science of Life","volume":"37 1","pages":"74 - 80"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70684350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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