The assessment of antibiotic resistance and related genes of foodborne Acinetobacter spp. and the analysis of whether they are genetically related to clinical infection-agent strains are crucial in terms of sustainability of food safety. The study at hand investigated antibiotic resistance, aminoglycoside-modifying enzyme (AME), and colistin resistance (PmrA) genes, clonal relationships while evaluating a possible correlation between antibiotic resistance and related genes between 27 foodborne and 50 clinical Acinetobacter spp. in Turkey. Antimicrobial susceptibilities, (AME), PmrA genes, and clonal relatedness of the strains were performed by disc diffusion, PCR, and Pulsed Field gel Electrophoresis (PFGE) methods, respectively. The aph-AI, aph-6, anth (3’’)-I, aadA1, aadB and PmrA genes were found as (24, 48%), (11, 22%), (7, 14%), (1, 2%), (2, 4%), and (46, 92%) respectively in clinical strains. This rate was found as (14, 51.9%), (16, 59.3%), (19, 70.4%), (2, 7.4%), (0, 0%), and (27, 100%) respectively in foodborne isolates. A positive correlation existed between the number of aph-AI gene positivity and trimethoprim-sulfamethoxazole and gentamycin resistance; anth (3’’)-I gene positivity, and colistin resistance; PmrA gene positivity and piperacillin-tazobactam, ceftazidime, meropenem, amikacin, and imipenem resistance in clinical strains (P
{"title":"Molecular characterization of antibiotic resistance, aminoglycoside and PmrA genes among foodborne and clinical Acinetobacter spp.","authors":"Mevhibe Terkuran, Z. Erginkaya, F. Köksal","doi":"10.33988/auvfd.1113432","DOIUrl":"https://doi.org/10.33988/auvfd.1113432","url":null,"abstract":"The assessment of antibiotic resistance and related genes of foodborne Acinetobacter spp. and the analysis of whether they are genetically related to clinical infection-agent strains are crucial in terms of sustainability of food safety. The study at hand investigated antibiotic resistance, aminoglycoside-modifying enzyme (AME), and colistin resistance (PmrA) genes, clonal relationships while evaluating a possible correlation between antibiotic resistance and related genes between 27 foodborne and 50 clinical Acinetobacter spp. in Turkey. Antimicrobial susceptibilities, (AME), PmrA genes, and clonal relatedness of the strains were performed by disc diffusion, PCR, and Pulsed Field gel Electrophoresis (PFGE) methods, respectively. The aph-AI, aph-6, anth (3’’)-I, aadA1, aadB and PmrA genes were found as (24, 48%), (11, 22%), (7, 14%), (1, 2%), (2, 4%), and (46, 92%) respectively in clinical strains. This rate was found as (14, 51.9%), (16, 59.3%), (19, 70.4%), (2, 7.4%), (0, 0%), and (27, 100%) respectively in foodborne isolates. A positive correlation existed between the number of aph-AI gene positivity and trimethoprim-sulfamethoxazole and gentamycin resistance; anth (3’’)-I gene positivity, and colistin resistance; PmrA gene positivity and piperacillin-tazobactam, ceftazidime, meropenem, amikacin, and imipenem resistance in clinical strains (P","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69596507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mastitis is one of the most important problems of the dairy industry because of the economic losses due to decreases in milk yield and in milk quality. Subclinical mastitis is a form of mastitis that has no macroscopic inflammation in the mammary gland and visible change in milk, and approximately 70-80% of milk losses caused by mastitis are due to subclinical mastitis. Many previous studies, screening the mastitis and the isolation and identification of microorganisms in collected milk samples, have been conducted in dairy cattle at the regional level in Turkey. Since many studies have been performed to predict the prevalence of subclinical mastitis and to determine the factors affecting the disease, systematic review or meta-analysis studies on the subject have been required. In this study, it was aimed to determine the prevalence of subclinical mastitis obtained from 38 studies conducted in Turkey between 1988 and 2019 by meta-analysis method and to calculate common prevalence. The estimated prevalence of subclinical mastitis in the studies were evaluated separately as cow-based (11182 cows in 37 studies) and udder quarter-based (48990 udder quarters in 33 studies). According to the results, the common prevalence of subclinical mastitis was calculated as 0.49 (95% CI: 0.41-0.56) in cow-based studies and 0.32 (95% CI: 0.26-0.37) in quarter based- based studies. As a result of this study, the results of individual studies on the prevalence of subclinical mastitis in dairy cows in Turkey were combined with the meta-analysis method and a more precise estimate of the prevalence was obtained.
{"title":"Meta-analysis and meta-regression of subclinical mastitis prevalences of dairy cattle in Turkey","authors":"E. Çelik, A. Akçay","doi":"10.33988/auvfd.1155601","DOIUrl":"https://doi.org/10.33988/auvfd.1155601","url":null,"abstract":"Mastitis is one of the most important problems of the dairy industry because of the economic losses due to decreases in milk yield and in milk quality. Subclinical mastitis is a form of mastitis that has no macroscopic inflammation in the mammary gland and visible change in milk, and approximately 70-80% of milk losses caused by mastitis are due to subclinical mastitis. Many previous studies, screening the mastitis and the isolation and identification of microorganisms in collected milk samples, have been conducted in dairy cattle at the regional level in Turkey. Since many studies have been performed to predict the prevalence of subclinical mastitis and to determine the factors affecting the disease, systematic review or meta-analysis studies on the subject have been required. \u0000In this study, it was aimed to determine the prevalence of subclinical mastitis obtained from 38 studies conducted in Turkey between 1988 and 2019 by meta-analysis method and to calculate common prevalence. The estimated prevalence of subclinical mastitis in the studies were evaluated separately as cow-based (11182 cows in 37 studies) and udder quarter-based (48990 udder quarters in 33 studies). According to the results, the common prevalence of subclinical mastitis was calculated as 0.49 (95% CI: 0.41-0.56) in cow-based studies and 0.32 (95% CI: 0.26-0.37) in quarter based- \u0000based studies. As a result of this study, the results of individual studies on the prevalence of subclinical mastitis in dairy cows in Turkey were combined with the meta-analysis method and a more precise estimate of the prevalence was obtained.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69597594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to evaluate and compare the varroacidal efficacy and mite mortality dynamic during autumn treatment of honey bee colonies situated at two exper-imental areas, treated with three available authorized veterinary medicinal substances. In the autumn of 2017 clinical studies were conducted on the efficiency of the three acari-cidal combinations (AC) - one, based on 3. 6 mg flumethrin/strip (AC-1) and two contents essential oils (first one with composition: 5 g thymol and 2 g peppermint oil/plate (AC-2), and second one with composition: 4 g thymol and 2 g peppermint oil/plаte (AC-3). For control treatment we used the product containing coumaphos and additive with oxalic acid. After 35 days of flumethrin-strips exposure, an efficiency of 94.5% and 87.82% were achieved in the apiaries in Boychinovtsi and Zlatiya, respectively. The efficiencies of the other tested combinations - 5 g thymol/ and 2 g peppermint oil plate and 4 g thymol and 2 g peppermint oil /plate for 45 days were also high in the apiary in Zlatiya (AC-2 - 97% and for AC-3 - 95%) and in Boychinovtsi were 91% for AC-2 and 80% for AC-3, respectively. The results of the experiments showed the absence of resistance to the tested substances.
{"title":"Clinical trial of the efficiency of three different compositions of acaricidal substances against varoosis in honey bee colonies","authors":"","doi":"10.33988/auvfd.1033097","DOIUrl":"https://doi.org/10.33988/auvfd.1033097","url":null,"abstract":"The aim of this study was to evaluate and compare the varroacidal efficacy and mite mortality dynamic during autumn treatment of honey bee colonies situated at two exper-imental areas, treated with three available authorized veterinary medicinal substances. In the autumn of 2017 clinical studies were conducted on the efficiency of the three acari-cidal combinations (AC) - one, based on 3. 6 mg flumethrin/strip (AC-1) and two contents essential oils (first one with composition: 5 g thymol and 2 g peppermint oil/plate (AC-2), and second one with composition: 4 g thymol and 2 g peppermint oil/plаte (AC-3). For control treatment we used the product containing coumaphos and additive with oxalic acid. After 35 days of flumethrin-strips exposure, an efficiency of 94.5% and 87.82% were achieved in the apiaries in Boychinovtsi and Zlatiya, respectively. The efficiencies of the other tested combinations - 5 g thymol/ and 2 g peppermint oil plate and 4 g thymol and 2 g peppermint oil /plate for 45 days were also high in the apiary in Zlatiya (AC-2 - 97% and for AC-3 - 95%) and in Boychinovtsi were 91% for AC-2 and 80% for AC-3, respectively. The results of the experiments showed the absence of resistance to the tested substances.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69592615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sencer Uygur, A. Doğan, Fati Qoti, Merve Yildiz, A. Bumi̇n
The study material consisted of an 8 year old mixed breed female cat brought with lameness. As a result of clinical and radiographic evaluations, luxation in the left elbow joint and proximal diaphyseal transversal fracture in the radius were detected. The Reduction of the elbow joint was achieved with the cerclage transarticular external fixation (STEF) method. Fixation of the radius fracture was performed using the paraosseous clamp cerclage method. Radiographs of the relevant extremity were taken immediately post-operative, on the 10th day and the 1st year, and the degree of range of motion was determined. This method is thought to be an alternative method that can be used in the treatment of elbow luxation.
{"title":"Treatment of Elbow Luxation and Radius Fracture with Cerclage Transarticular External Fixation and Paraosseous Clamp Cerclage in a Cat","authors":"Sencer Uygur, A. Doğan, Fati Qoti, Merve Yildiz, A. Bumi̇n","doi":"10.33988/auvfd.1158729","DOIUrl":"https://doi.org/10.33988/auvfd.1158729","url":null,"abstract":"The study material consisted of an 8 year old mixed breed female cat brought with lameness. As a result of clinical and radiographic evaluations, luxation in the left elbow joint and proximal diaphyseal transversal fracture in the radius were detected. The Reduction of the elbow joint was achieved with the cerclage transarticular external fixation (STEF) method. Fixation of the radius fracture was performed using the paraosseous clamp cerclage method. Radiographs of the relevant extremity were taken immediately post-operative, on the 10th day and the 1st year, and the degree of range of motion was determined. This method is thought to be an alternative method that can be used in the treatment of elbow luxation.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47889060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Salmonella Enteritidis and Salmonella Typhimurium are the most common serovars observed in human salmonellosis while contaminated poultry products are the major source of Salmonella transmission to humans. Therefore, high pathogenicity of poultry-originated S. Enteritidis and S. Typhimurium strains poses a serious risk to human health. This study analyzed the virulence genes of broiler chicken-originated S. Enteritidis and S. Typhimurium strains. SipA, sipD, sopB, sopD, sopE, sopE2, sitC, sifA, ssaR, spvC and pefA genes were investigated in a total of 137 strains consisting of 105 S. Enteritidis and 32 S. Typhimurium. Nine strains (6.6%) had all genes. No negative strain was detected for all genes. SopE was found in all strains (100%). SitC (89.1%), ssaR (83.9%), sipA (70.1%), sipD (73.0%), sopE2 (68.6%), spvC (68.6%) and pefA (73.0%) were also highly prevalent. Noticeable differences were observed between serovars in terms of sopE2, spvC and pefA prevalence: 77.1%, 80% and 82.9%, respectively, of S. Enteritidis strains were sopE2, spvC and pefA positive while 40.6%, 31.3% and 40.6% of S. Typhimurium strains were positive. This finding indicates that S. Enteritidis is more frequent than S. Typhimurium in poultry populations thanks to higher virulence. Based on virulence gene distribution, the strains were divided into 44 different virulence genotypes, with the major genotype 4 (15.3%) carrying 8 of the 11 genes. The majority of strains (75.9%) were positive for at least 6 genes. S. Enteritidis and S. Typhimurium strains were highly virulent and pose a threat as a zoonotic infection.
{"title":"Molecular characterization of virulence genes in poultry-originated Salmonella Enteritidis and Salmonella Typhimurium","authors":"Seyyide SARIÇAM İNCE, M. Akan","doi":"10.33988/auvfd.1157022","DOIUrl":"https://doi.org/10.33988/auvfd.1157022","url":null,"abstract":"Salmonella Enteritidis and Salmonella Typhimurium are the most common serovars observed in human salmonellosis while contaminated poultry products are the major source of Salmonella transmission to humans. Therefore, high pathogenicity of poultry-originated S. Enteritidis and S. Typhimurium strains poses a serious risk to human health. This study analyzed the virulence genes of broiler chicken-originated S. Enteritidis and S. Typhimurium strains. SipA, sipD, sopB, sopD, sopE, sopE2, sitC, sifA, ssaR, spvC and pefA genes were investigated in a total of 137 strains consisting of 105 S. Enteritidis and 32 S. Typhimurium. Nine strains (6.6%) had all genes. No negative strain was detected for all genes. SopE was found in all strains (100%). SitC (89.1%), ssaR (83.9%), sipA (70.1%), sipD (73.0%), sopE2 (68.6%), spvC (68.6%) and pefA (73.0%) were also highly prevalent. Noticeable differences were observed between serovars in terms of sopE2, spvC and pefA prevalence: 77.1%, 80% and 82.9%, respectively, of S. Enteritidis strains were sopE2, spvC and pefA positive while 40.6%, 31.3% and 40.6% of S. Typhimurium strains were positive. This finding indicates that S. Enteritidis is more frequent than S. Typhimurium in poultry populations thanks to higher virulence. Based on virulence gene distribution, the strains were divided into 44 different virulence genotypes, with the major genotype 4 (15.3%) carrying 8 of the 11 genes. The majority of strains (75.9%) were positive for at least 6 genes. S. Enteritidis and S. Typhimurium strains were highly virulent and pose a threat as a zoonotic infection.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69597637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammad Hassanpour, R. Partovi, Mohammad Hassan Shahavi
This study investigated the effects of Trachyspermum copticum essential oil nanoemulsion (TCEO-NE) coating on chemical, microbial and sensory changes of Oncorhynchus aguabonita fillets during storage at 4 °C. The components of T. copticum EO were identified using gas chromatography-mass spectrometry. TCEO-NE was prepared by ultrasonic method and its properties were identified. Fresh O. aguabonita fillets were immersed in TCEO-NE and stored at 4 °C. Chemical (pH, TVB-N, peroxide and TBARS), microbiological (total aerobic mesophilic bacteria, psychrotrophs, enterobacteriaceae, lactic acid bacteria and Staphylococcus aureus) and sensory analyses (color, odor and taste) of fish fillets were evaluated on days 0, 2, 4, 6, 8 and 11. Thymol, limonene and alpha-terpinene were the major compounds in T. copticum EO. The droplet size of TCEO-NE was 580.30 nm and PDI was 0.46. The control group exceeded the peroxide limit on day 6, while TCEO-NE 500.00 and 666.66 did so on day 8. The TBARS value in was 0.57 mg MA/kg on day 0 which reached 4.76 mg MA/kg in the control group and 2.90 mg MA/kg in TCEO-NE 666.66 after 11 days at 4 °C. Aerobic mesophilic count in the control group exceeded the permissible level on day 6 and, in TCEO-NE 500.00 and 666.66, on day 8, therefore the shelf life of fish fillets was improved by two days. On the grounds of the favorable properties of TCEO-NE and its positive effects on chemical, microbial and sensory changes in fish fillets, it can be used as a natural food additive.
{"title":"Shelf life extension of Oncorhynchus aguabonita fillets based on Trachyspermum copticum essential oil nanoemulsion coating during storage at 4 °C","authors":"Mohammad Hassanpour, R. Partovi, Mohammad Hassan Shahavi","doi":"10.33988/auvfd.1014845","DOIUrl":"https://doi.org/10.33988/auvfd.1014845","url":null,"abstract":"This study investigated the effects of Trachyspermum copticum essential oil nanoemulsion (TCEO-NE) coating on chemical, microbial and sensory changes of Oncorhynchus aguabonita fillets during storage at 4 °C. The components of T. copticum EO were identified using gas chromatography-mass spectrometry. TCEO-NE was prepared by ultrasonic method and its properties were identified. Fresh O. aguabonita fillets were immersed in TCEO-NE and stored at 4 °C. Chemical (pH, TVB-N, peroxide and TBARS), microbiological (total aerobic mesophilic bacteria, psychrotrophs, enterobacteriaceae, lactic acid bacteria and Staphylococcus aureus) and sensory analyses (color, odor and taste) of fish fillets were evaluated on days 0, 2, 4, 6, 8 and 11. Thymol, limonene and alpha-terpinene were the major compounds in T. copticum EO. The droplet size of TCEO-NE was 580.30 nm and PDI was 0.46. The control group exceeded the peroxide limit on day 6, while TCEO-NE 500.00 and 666.66 did so on day 8. The TBARS value in was 0.57 mg MA/kg on day 0 which reached 4.76 mg MA/kg in the control group and 2.90 mg MA/kg in TCEO-NE 666.66 after 11 days at 4 °C. Aerobic mesophilic count in the control group exceeded the permissible level on day 6 and, in TCEO-NE 500.00 and 666.66, on day 8, therefore the shelf life of fish fillets was improved by two days. On the grounds of the favorable properties of TCEO-NE and its positive effects on chemical, microbial and sensory changes in fish fillets, it can be used as a natural food additive.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69591994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Ahlat, Özgür Özöner, Kürşat Fi̇li̇kci̇, S. ATALAY VURAL
In this case, mycobacteriosis in a Nile crocodile’s tissues, which were sent from a private zoo, was described pathomorphologically and immunohistochemically. Macroscopically; multifocal, greyish-white areas were observed in the sent lung, liver and spleen. Histologically, a large number of well-demarcated necrotic areas were seen. These areas included nuclei debris locally. Moderate inflammatory cells and a couple of multinucleated giant cells surrounded the necrotic cores. Numerous acid-fast bacilli were detected by Ziehl-Neelsen staining method. Immunolabelling for both Mycobacterium bovis and anti-BCG antibodies was positive in each tissue.
{"title":"Mycobacteriosis in a Nile crocodile (Crocodylus niloticus) from Turkey","authors":"O. Ahlat, Özgür Özöner, Kürşat Fi̇li̇kci̇, S. ATALAY VURAL","doi":"10.33988/auvfd.1139830","DOIUrl":"https://doi.org/10.33988/auvfd.1139830","url":null,"abstract":"In this case, mycobacteriosis in a Nile crocodile’s tissues, which were sent from a private zoo, was described pathomorphologically and immunohistochemically. Macroscopically; multifocal, greyish-white areas were observed in the sent lung, liver and spleen. Histologically, a large number of well-demarcated necrotic areas were seen. These areas included nuclei debris locally. Moderate inflammatory cells and a couple of multinucleated giant cells surrounded the necrotic cores. Numerous acid-fast bacilli were detected by Ziehl-Neelsen staining method. Immunolabelling for both Mycobacterium bovis and anti-BCG antibodies was positive in each tissue.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69597214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gülay Çi̇ftci̇, Alper Çi̇ftci̇, Metin Çenesi̇z, Burcu Onuk, S. Çenesi̇z, T. Gülhan
Hypercholesterolemia is a very common health problem in the world. In this study, it was aimed to investigate the effects of administering atorvastatin and Lactobacillus acidophilus probiotic on cholesterol metabolism, and formation of neurosterides and myelin. Five groups were formed in the study. Group-1 was fed with standard rat chow as a control group. Group-2 was designated as hypercholosterolemi group and fed with cholesterol added rat chow. Group-3 was fed with cholesterol and atorvastatin. L.acidophilus probiotic was given in the last four weeks of the experiment to Group-4. L.acidophilus and atorvastatin were given together for the last four weeks to the Group-5. At the end of the trial, some biochemical parameters were determined by autoanalyzer device and ELISA. LDL receptor, HMG-CoA reductase, GAPDH genes were determined by RT-PCR. In the case of adding cholesterol to the diet, total cholesterol in the serum, LDL-cholesterol level increased, HDL-cholesterol level decreased, liver enzyme activity increased, Ox-LDL level increased significantly in the brain, testosterone, progesterone, MBP level, nNOS activity were significantly increased. GAPDH gene gave bands at the same intensities in brain and liver of in all groups. When compared with GAPDH, band intensities of the LDL receptor, and HMG-CoA reductase genes were decreased. It was determined that the hypocholesteric effect of the combination of statin and probiotic is better and neurosterides have a positive contribution to the level of serotonin hormone. In conclusion, L. acidophilus probiotic can be recommended as an alternative treatment with atorvastatin in the treatment of hypercholesterolemia.
{"title":"Deneysel hiperkolesterolemide Atorvastatin ve Lactobacillus acidophilus'un kolesterol metabolizmasına etkisi","authors":"Gülay Çi̇ftci̇, Alper Çi̇ftci̇, Metin Çenesi̇z, Burcu Onuk, S. Çenesi̇z, T. Gülhan","doi":"10.33988/auvfd.1133218","DOIUrl":"https://doi.org/10.33988/auvfd.1133218","url":null,"abstract":"Hypercholesterolemia is a very common health problem in the world. In this study, it was aimed to investigate the effects of administering atorvastatin and Lactobacillus acidophilus probiotic on cholesterol metabolism, and formation of neurosterides and myelin. Five groups were formed in the study. Group-1 was fed with standard rat chow as a control group. Group-2 was designated as hypercholosterolemi group and fed with cholesterol added rat chow. Group-3 was fed with cholesterol and atorvastatin. L.acidophilus probiotic was given in the last four weeks of the experiment to Group-4. L.acidophilus and atorvastatin were given together for the last four weeks to the Group-5. At the end of the trial, some biochemical parameters were determined by autoanalyzer device and ELISA. LDL receptor, HMG-CoA reductase, GAPDH genes were determined by RT-PCR. In the case of adding cholesterol to the diet, total cholesterol in the serum, LDL-cholesterol level increased, HDL-cholesterol level decreased, liver enzyme activity increased, Ox-LDL level increased significantly in the brain, testosterone, progesterone, MBP level, nNOS activity were significantly increased. GAPDH gene gave bands at the same intensities in brain and liver of in all groups. When compared with GAPDH, band intensities of the LDL receptor, and HMG-CoA reductase genes were decreased. It was determined that the hypocholesteric effect of the combination of statin and probiotic is better and neurosterides have a positive contribution to the level of serotonin hormone. In conclusion, L. acidophilus probiotic can be recommended as an alternative treatment with atorvastatin in the treatment of hypercholesterolemia.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69597031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aims to describe a novel design of an acrylic external skeletal fixator system for stabilizing epiphyseal/metaphyseal fractures with or without articular involvement and evaluate its efficiency in 13 cases. Client-owned five cats and eight dogs with epiphyseal/metaphyseal fractures were included in this study. Cross pins with or without a transcortical pin were included in "J" shaped acrylic ESF, and this novel technique was called the Tie-cross ESF. The functional use of the extremity was evaluated, and the joint range of motion was assessed and compared with the contralateral side. Radiographs were evaluated for bone healing and possible complications. The time of the first use of the extremities changed from the day of surgery to the second day. Fracture healing occurred in 35-69 days, and ESFs were removed. No persistent lameness was observed, and total functional recovery was provided in all cases. Fixation of epiphyseal and metaphyseal fractures can be challenging, especially when it involves the articular surface. Including cross-pins in an acrylic ESF (Tie-cross ESF) is useful and can be considered an alternative technique for the stabilization of these fractures. This technique encourages the patient to use the extremity during the fracture healing and enables joint functions; therefore, additional physical therapy will not be necessary.
{"title":"Tie-Cross Acrylic External Skeletal Fixator: Technique and 13 cases","authors":"G. Yeşi̇lovali̇, M. A. Çeti̇nkaya","doi":"10.33988/auvfd.1173948","DOIUrl":"https://doi.org/10.33988/auvfd.1173948","url":null,"abstract":"This study aims to describe a novel design of an acrylic external skeletal fixator system for stabilizing epiphyseal/metaphyseal fractures with or without articular involvement and evaluate its efficiency in 13 cases. Client-owned five cats and eight dogs with epiphyseal/metaphyseal fractures were included in this study. Cross pins with or without a transcortical pin were included in \"J\" shaped acrylic ESF, and this novel technique was called the Tie-cross ESF. The functional use of the extremity was evaluated, and the joint range of motion was assessed and compared with the contralateral side. Radiographs were evaluated for bone healing and possible complications. The time of the first use of the extremities changed from the day of surgery to the second day. Fracture healing occurred in 35-69 days, and ESFs were removed. No persistent lameness was observed, and total functional recovery was provided in all cases. Fixation of epiphyseal and metaphyseal fractures can be challenging, especially when it involves the articular surface. Including cross-pins in an acrylic ESF (Tie-cross ESF) is useful and can be considered an alternative technique for the stabilization of these fractures. This technique encourages the patient to use the extremity during the fracture healing and enables joint functions; therefore, additional physical therapy will not be necessary.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69597306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mesenchymal stem cells are adult stem cells that have the ability to differentiate into osteogenic, chondrogenic, adipogenic and myogenic lineages. In the field of orthopedics and traumatology, mesenchymal stem cells in combination with biomaterials are used especially for the treatment of bone fractures and diseases in both humans and animals. The purpose of this study is to promote growth, proliferation and osteogenic differentiation of mesenchymal stem cells that were isolated from the adipose tissue of canines on B-TCP (Beta-tricalcium phosphate) and B-TCP/Collagen biomaterials. MTT analysis was performed to test the cell adhesion and proliferation on B-TCP and B-TCP/Collagen biomaterials that were used to mimic the extracellular matrix of three-dimensional bone tissue. Scanning electron microscope analysis was performed to show general surface characters of B-TCP and B-TCP /Collagen biomaterials. The osteoinductive capacities of the B-TCP and B-TCP/Collagen biomaterials were determined by alkaline phosphatase and Von Kossa stainings, and RT-PCR analysis. The ALP activity of the B-TCP/Col containing material was significantly higher than the B-TCP on the first days. In terms of gene expression, there were no significant differences except 14th-day SPARC gene expression. The results of Von Kossa staining indicate that B-TCP/Col has above the desired level degradation capacity. As a result of this research, although it is advantageous in terms of alkaline phosphatase activity and osteogenic gene expression compared to B-TCP material, it is thought that B-TCP/Collagen biomaterial should be developed for use in bone tissue engineering due to its high degradation property.
{"title":"Osteogenic Differentiation of Canine Adipose Derived Mesenchymal Stem Cells on B-TCP and B-TCP/Collagen Biomaterials","authors":"Özge Özgenç, A. Özen","doi":"10.33988/auvfd.1130705","DOIUrl":"https://doi.org/10.33988/auvfd.1130705","url":null,"abstract":"Mesenchymal stem cells are adult stem cells that have the ability to differentiate into osteogenic, chondrogenic, adipogenic and myogenic lineages. In the field of orthopedics and traumatology, mesenchymal stem cells in combination with biomaterials are used especially for the treatment of bone fractures and diseases in both humans and animals. The purpose of this study is to promote growth, proliferation and osteogenic differentiation of mesenchymal stem cells that were isolated from the adipose tissue of canines on B-TCP (Beta-tricalcium phosphate) and B-TCP/Collagen biomaterials. MTT analysis was performed to test the cell adhesion and proliferation on B-TCP and B-TCP/Collagen biomaterials that were used to mimic the extracellular matrix of three-dimensional bone tissue. Scanning electron microscope analysis was performed to show general surface characters of B-TCP and B-TCP /Collagen biomaterials. The osteoinductive capacities of the B-TCP and B-TCP/Collagen biomaterials were determined by alkaline phosphatase and Von Kossa stainings, and RT-PCR analysis. The ALP activity of the B-TCP/Col containing material was significantly higher than the B-TCP on the first days. In terms of gene expression, there were no significant differences except 14th-day SPARC gene expression. The results of Von Kossa staining indicate that B-TCP/Col has above the desired level degradation capacity. As a result of this research, although it is advantageous in terms of alkaline phosphatase activity and osteogenic gene expression compared to B-TCP material, it is thought that B-TCP/Collagen biomaterial should be developed for use in bone tissue engineering due to its high degradation property.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2022-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69596293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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