Animal Reproduction Science最新文献_第9页

Effects of GnRH administration on pregnancy rate and litter size in ewes subjected to a fixed time insemination program during the breeding season GnRH给药对妊娠率和产仔数的影响，母羊在繁殖季节接受固定时间的授精程序。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-09-30 DOI: 10.1016/j.anireprosci.2025.108010

Baoyue Cui , Aibibaimu Hapaer , Xinglong Wu , Yiyong Liu , Xiangyun Li

The objective of this study was to compare the effects of GnRH administration at sponge insertion, insemination, and day 7 post-insemination on pregnancy rate and litter size in ewes subjected to a fixed time insemination program during the breeding season. Estrus was synchronized in 2740 adult ewes at four experimental sites. An intravaginal sponge impregnated with flurogestone acetate was left in the vagina for 12 days followed by an injection of 330 IU of eCG at sponge removal. Each ewe was in seminated twice at 52 h and 64 h after sponge removal. In the Insertion group, the Insemination group, and the Post-insemination group, ewes were given an intramuscular administration of 25 μg of the GnRH agonist triptorelin at sponge insertion, insemination, and day 7 post-insemination, respectively. No GnRH administration was given in the Control group. Pregnancy rates and litter size were calculated after all deliveries were completed. The results showed that the pregnancy rates in the Insertion group were significantly higher compared to those in the Control group, the Insemination group, and the Post-insemination group. The litter size in the Insertion group and the Insemination group were not significantly different and were significantly higher compared to those in the Control group and the Post-insemination group. In conclusion, the fixed time artificial insemination program, in combination with GnRH administration at sponge insertion, is a simple and potentially effective strategy for increasing fertility in estrus-synchronized ewes during the breeding season.

本研究的目的是比较GnRH在海绵植入、授精和授精后第7天对母羊妊娠率和产仔数的影响，母羊在繁殖季节接受固定时间授精。在四个试验点对2740只母羊进行了发情同步。将经醋酸氟孕酮浸渍的阴道内海绵留在阴道内12天，然后在海绵取出时注射330 IU eCG。每只母羊在去除海绵后分别于52 h和64 h进行两次授精。在插入组、授精组和授精后组，母羊分别在海绵插入、授精和授精后第7天肌肉注射GnRH激动剂triptorelin 25 μg。对照组未给予GnRH治疗。在所有分娩完成后计算怀孕率和产仔数。结果显示，插入组的妊娠率明显高于对照组、人工授精组和人工授精后组。插入组和授精组产仔数差异不显著，显著高于对照组和授精后组。综上所述，固定时间人工授精，结合海绵植入时给药GnRH，是一种在繁殖季节提高发情同步母羊生育力的简单而有效的策略。

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引用次数: 0

Broodstock age and diet as determinants of gamete quality and early development in hatchery-reared grayling (Thymallus thymallus) 种鱼龄和日粮对育成灰鲑配子质量和早期发育的影响

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-09-29 DOI: 10.1016/j.anireprosci.2025.108008

Tjaša Kodela , Alenka Levart , Ákos Horváth , Ida Djurdjevič , Simona Sušnik Bajec

Grayling (Thymallus thymallus) populations have declined significantly in European waters over past few decades. One of key conservation strategies involves stocking rivers with hatchery-reared fish. To improve the grayling rearing processes in hatcheries, this two-year study investigates the effects of broodstock age (3+ and 4+) and different commercial feeds on gamete quality, measured through egg biochemical composition, fertile egg proportion, sperm concentration and mobility, and early embryonic development in grayling. Broodstock age emerged as the dominant factor influencing reproductive success, with older females demonstrating higher fecundity and larger eggs. In addition, eggs of first-time spawners exhibited higher DHA/EPA ratios, which may be linked to early mortality syndromes; however, no differences in embryo survival were observed between age groups. Age also affected sperm quality, with higher progressive motility and velocity, but lower concentration in older males. Feed had a moderate effect on gamete quality, influencing the biochemical composition of eggs, including fatty acid profiles, and sperm concentration. Combined with reproductive performance, it was concluded that none of the tested feeds fully met the species' nutritional requirements for optimal reproduction. The average fertile egg proportion was 70 %, survival to the eyed egg stage was 25 %, and the hatching rate of larvae was below 10 %. Growth differences were observed, with males responding positively to carbohydrate-rich diets. Eggs from females fed high-carbohydrate diets exhibited altered n-3/n-6 PUFA ratios and reduced essential fatty acid levels. The findings underscore the necessity for tailored broodstock feed for grayling, which supports both growth and reproduction.

在过去的几十年里，欧洲水域的灰鱼（胸腺）数量显著下降。关键的保护策略之一是在河流中放养孵化场饲养的鱼。为了改进孵化场对灰鲑的饲养工艺，本研究通过对灰鲑的卵生化组成、受精卵比例、精子浓度和流动性以及早期胚胎发育进行测量，研究了3+龄和4+龄和不同商业饲料对配子质量的影响。母鱼年龄成为影响繁殖成功的主要因素，年龄较大的母鱼表现出更高的繁殖力和更大的卵。此外，首次产卵的卵表现出较高的DHA/EPA比率，这可能与早期死亡综合征有关；然而，胚胎存活率在不同年龄组之间没有差异。年龄也会影响精子质量，老年男性的精子活力和速度较高，但浓度较低。饲料对配子质量有中等影响，影响卵子的生化组成，包括脂肪酸谱和精子浓度。结合繁殖性能，试验饲料均不能完全满足该物种最佳繁殖所需的营养需求。平均受精卵比例为70 %，至眼卵期成活率为25 %，幼虫孵化率低于10 %。观察到生长差异，雄性对富含碳水化合物的饮食反应积极。饲喂高碳水化合物饲料的雌性卵显示出n-3/n-6 PUFA比率的改变和必需脂肪酸水平的降低。研究结果强调了为灰鲑量身定制饵料的必要性，这既支持了灰鲑的生长，又支持了灰鲑的繁殖。

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引用次数: 0

Seminal extracellular vesicles influence porcine spermatozoa physiology by modulating key functional parameters 精子细胞外囊泡通过调节关键功能参数影响猪精子生理。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-09-27 DOI: 10.1016/j.anireprosci.2025.108009

Ana Parra , Francisco E. Martín-Cano , Pablo Martínez-Díaz , Patricia Panales , Xiomara Lucas , Jordi Roca , Fernando J. Peña , Isabel Barranco

Seminal plasma (SP) contains a heterogeneous population of extracellular vesicles (EVs) recognized as key modulators of sperm function. However, the specific functional roles of each seminal EV (sEV) subset remain poorly understood. This study aimed to evaluate the interaction of two sized sEV subsets (small [S-sEVs] and large [L-sEVs]) with pig liquid-stored spermatozoa under different pH conditions and their effect on specific sperm functional parameters. Seminal EV subsets were isolated from SP samples using size exclusion chromatography and characterized following the MISEV2023 guidelines. Semen samples were incubated with each sEV subset or without sEVs (control) for 6 h at 37 ºC, 100 % humidity and 5 % CO₂ under different pH conditions (6.5, 7.0, or 7.5). Sperm functional parameters were assessed by flow cytometry (Cytoflex®S and LX, Beckman Coulter), under capacitating and non-capacitating conditions. Confocal microscopy revealed that both sEV subsets bound to and were internalized by spermatozoa as early as 30 min after incubation, regardless of pH. Flow cytometry revealed that both sEVs decreased reactive oxygen species production (P ≤ 0.0001), mitochondrial membrane potential (P ≤ 0.0001) and mitochondrial O₂^•⁻ levels (P ≤ 0.01) and increased apoptosis (active caspase-3) in viable spermatozoa (P ≤ 0.0001). However, the influence of sEV on acrosome integrity in viable sperm was time- and condition-dependent (P ≤ 0.05). This study showed that both S- and L-sEVs interact with porcine spermatozoa across a range of physiological pH conditions. This interaction is reflected by decreased oxidative stress and mitochondrial activity, as well as increased apoptosis in spermatozoa.

精浆（SP）含有异质性的细胞外囊泡（ev），被认为是精子功能的关键调节剂。然而，每个种子EV （sEV）子集的具体功能角色仍然知之甚少。本研究旨在评估两种大小的sEV亚群（小[s -sEV]和大[l -sEV]）在不同pH条件下与猪液体储存精子的相互作用及其对精子特定功能参数的影响。使用大小排除色谱法从SP样品中分离出种子EV亚群，并按照MISEV2023指南进行表征。精液样本在37ºC， 100 %湿度和5 % CO₂条件下（6.5,7.0或7.5）与每个sEV子集或不含sEV（对照）孵育6 h。通过流式细胞术（Cytoflex®S和LX， Beckman Coulter）评估精子在获能和非获能条件下的功能参数。共焦显微镜显示绑定到子集签订和内化的精子早在孵化后30 min,无论博士流式细胞术显示,股票减少活性氧生产(P ≤0.0001 ),线粒体膜电位(P ≤0.0001 )和线粒体O₂•⁻水平(P ≤0.01 )和增加细胞凋亡(活跃caspase-3)可行的精子(P ≤ 0.0001)。sEV对活精子顶体完整性的影响具有时间和条件依赖性（P ≤ 0.05）。这项研究表明，S- sev和l - sev在一系列生理pH条件下与猪精子相互作用。这种相互作用反映在氧化应激和线粒体活性降低，以及精子细胞凋亡增加。

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引用次数: 0

Spermidine supplementation protects porcine oocytes against triclosan-induced defects during maturation in vitro 补充亚精胺可保护猪卵母细胞在体外成熟过程中免受三氯生诱导的缺陷

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-09-22 DOI: 10.1016/j.anireprosci.2025.107999

Yang Gao , Dandan Zhang , Kaixiang Tan , Mengting Wu , Qixiang Tai , Guilan Zhu , Jinwu Chen , Changyin Zhou , Yong Zhu , Yunhai Zhang , Mianqun Zhang

Oocytes are highly susceptible to environmental pollutants, with triclosan (TCS)-a pervasive antimicrobial-known to bioaccumulate and impair reproductive function. Yet, the mechanisms of TCS-induced oocyte degeneration and effective protective strategies remain unclear. This study explored the protective role of spermidine (SPD) against TCS-induced meiotic disruption and fertilization defects in porcine oocytes, focusing on its antioxidant and anti-apoptotic mechanisms. We found that TCS exposure disrupted meiotic progression by inducing spindle defects, chromosome misalignment, mitochondrial dysfunction, oxidative stress, DNA damage, and apoptosis, ultimately impairing maturation and fertilization. SPD supplementation significantly alleviated these effects by stabilizing the cytoskeleton, reducing oxidative stress, and suppressing apoptosis, thereby restoring oocyte quality and developmental competence. These findings highlight SPD as a promising intervention to counteract pollutant-induced oocyte damage and safeguard female fertility.

卵母细胞对环境污染物非常敏感，众所周知，三氯生（TCS）是一种普遍存在的抗菌剂，会生物积累并损害生殖功能。然而，tcs诱导卵母细胞变性的机制和有效的保护策略尚不清楚。本研究探讨了亚精胺（SPD）对tcs诱导的猪卵母细胞减数分裂破坏和受精缺陷的保护作用，重点探讨了其抗氧化和抗凋亡机制。我们发现TCS暴露通过诱导纺锤体缺陷、染色体错位、线粒体功能障碍、氧化应激、DNA损伤和细胞凋亡来破坏减数分裂进程，最终损害成熟和受精。补充SPD可通过稳定细胞骨架、减少氧化应激和抑制细胞凋亡，从而恢复卵母细胞的质量和发育能力，显著缓解这些影响。这些发现强调SPD是一种有希望的干预措施，可以抵消污染物引起的卵母细胞损伤，保护女性生育能力。

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引用次数: 0

The cryoprotective effect of virgin coconut oil on boar sperm: Enhancing sperm quality and reproductive performance 初椰油对猪精子的冷冻保护作用：提高精子质量和生殖性能

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-09-22 DOI: 10.1016/j.anireprosci.2025.107998

Shu Ma , QianXi Liang , Zhili Li , Feng He , Ye Wang , Zhanwei Zhuang , Jian Zhou , Yunxiang Zhao , Fumei Chen

This study evaluated virgin coconut oil (VCO) as a natural cryoprotectant for boar sperm preservation. Duroc boar semen was cryopreserved with varying VCO concentrations (0.00 %, 0.01 %, 0.05 %, 0.10 %, 0.50 %, and 1.00 %). Comprehensive analysis revealed 0.1 % VCO optimally maintained post-thaw sperm quality, significantly improving motility, membrane integrity, acrosome integrity, and mitochondrial potential compared to controls. It also enhanced the activity of antioxidant enzymes, glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD). The optimal concentration was then used in artificial insemination (AI) trials to assess in vivo fertility. Metabolomic analysis identified significant alterations in lipid and energy metabolism pathways, with particular changes in N-acetylneuraminic acid and lysophosphatidylcholines that correlated with sperm quality improvements. These findings demonstrate VCO's effectiveness in protecting boar sperm during cryopreservation through multiple mechanisms including membrane stabilization, oxidative protection, and metabolic regulation. The 0.1 % VCO supplementation presents a practical solution for swine artificial insemination programs, offering both cryoprotective benefits and potential cost advantages over conventional additives.

本研究评价了初椰油（VCO）作为天然冷冻保护剂保存野猪精子的效果。杜洛克猪精液以不同的VCO浓度（0.00 %、0.01 %、0.05 %、0.10 %、0.50 %和1.00 %）冷冻保存。综合分析显示，与对照组相比，0.1% % VCO可最佳地维持解冻后精子质量，显著提高精子活力、膜完整性、顶体完整性和线粒体潜能。提高了抗氧化酶、谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）的活性。然后将最佳浓度用于人工授精（AI）试验，以评估体内生育能力。代谢组学分析发现了脂质和能量代谢途径的显著改变，特别是与精子质量改善相关的n -乙酰神经氨酸和溶血磷脂酰胆碱的变化。这些研究结果表明，VCO通过多种机制，包括膜稳定、氧化保护和代谢调节，在低温保存过程中保护猪精子的有效性。添加0.1% % VCO为猪人工授精计划提供了一个实用的解决方案，与传统添加剂相比，它既具有冷冻保护效果，又具有潜在的成本优势。

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引用次数: 0

Bovine β-defensin 129 (BBD129) polymorphisms associated with differential posttranslational O-glycosylation and phosphorylation modifications perturb the sperm functions and their fertilizing potential 牛β-防御素129 （BBD129）多态性与翻译后o糖基化和磷酸化修饰的差异有关，扰乱了精子功能和受精潜力

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-09-19 DOI: 10.1016/j.anireprosci.2025.107996

Subhash Solanki , Abhishek Kumar, Poonam Kashyap, Rakesh Kumar, Tirtha Kumar Datta

In our earlier finding BBD129 polymorphisms were differentially distributed in the distinct fertility cattle bulls and associated with altered post-translational modifications (PTMs), and sperm function. This study investigated the relationship between in-silico analysis of BBD129 polymorphism and previously used high-fertility (HF) and low-fertility (LF) cattle bulls’ sperm. Using CFDA-PI staining, it depicted similar frozen viable, moribund, and non-viable sperm population profiles between the HF and LF cattle bulls. Phosphorylation quantification via microscopy and flow cytometry revealed significantly higher serine and threonine phosphorylation in LF sperm, particularly in the tail and head regions, aligning with in-silico predictions and suggesting a link to LF bulls. Glycan localization was assessed using a panel of seven lectins, revealing distinct surface glycan patterns between HF and LF sperm. Lectins targeting O-linked glycans (MAL-II, Jacalin, SNA) showed significantly higher binding on HF sperm surface, whereas LCA and PNA were more abundant on LF sperm. These findings were consistently confirmed by both microscopy and flow cytometry. Notably, HF sperm exhibited a greater abundance of surface glycans, correlating with enhanced cervical mucus penetration ability in the cervical mucus penetration test (CMPT); HF sperm traveled an average of 52.77 mm versus 42.30 mm for LF sperm. This study demonstrates that BBD129 polymorphism influences critical PTMs, particularly glycosylation and phosphorylation, which in turn affect sperm membrane integrity, surface glycan composition, and functional competence in traversing the female reproductive tract. These molecular markers may serve as valuable indicators of bull fertility, providing insights for improving reproductive performance in cattle breeding programs.

在我们早期的研究中，BBD129多态性在不同生育能力的公牛中分布不同，并与翻译后修饰（PTMs）改变和精子功能相关。本研究探讨了BBD129多态性的计算机分析与先前使用的高育性（HF）和低育性（LF）牛精子的关系。使用CFDA-PI染色，它描绘了HF和LF牛之间相似的冷冻活精子、死精子和无活精子的种群特征。通过显微镜和流式细胞术进行的磷酸化量化显示，LF精子中丝氨酸和苏氨酸的磷酸化水平显著提高，尤其是在尾部和头部区域，这与计算机预测一致，表明与LF公牛有关。使用7种凝集素评估了聚糖定位，揭示了HF和LF精子之间不同的表面聚糖模式。针对o链聚糖的凝集素（MAL-II、Jacalin、SNA）在HF精子表面的结合率较高，而LCA和PNA在LF精子表面的结合率较高。显微镜和流式细胞术一致证实了这些发现。值得注意的是，在宫颈黏液渗透试验（CMPT）中，HF精子表现出更高的表面聚糖丰度，与增强的宫颈黏液渗透能力相关；HF精子平均移动52.77 mm，而LF精子平均移动42.30 mm。本研究表明，BBD129多态性影响关键的PTMs，特别是糖基化和磷酸化，进而影响精子膜的完整性、表面聚糖组成和穿越女性生殖道的功能能力。这些分子标记可以作为公牛生育能力的有价值的指标，为提高牛的繁殖性能提供见解。

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引用次数: 0

Automated color Doppler ultrasound analysis of bull reproductive tissues using a machine learning-based image processing algorithm 使用基于机器学习的图像处理算法对公牛生殖组织进行自动彩色多普勒超声分析

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-09-17 DOI: 10.1016/j.anireprosci.2025.107997

Joedson Dantas Gonçalves , Edilson Guimarães , Rubens Paes Arruda , Maria Emilia Franco Oliveira , Leonardo Machestropa Arikawa , Alexandre Rossetto Garcia

Color Doppler ultrasound is effective for studying tissue perfusion of various organs, but current analysis methods are subjective and time-consuming. This study aims to develop and validate an algorithm for analyzing color Doppler images of the bull's testis and pampiniform plexus. For the study, we selected 2304 color Doppler images (1152 for both the testicular parenchyma and the pampiniform plexus) that were analyzed by a conventional method (CON Group), by pixel separation and counting using Adobe Fireworks® and ImageJ®, or by an algorithm developed in Python version 3.10 (ALGO Group) that can be set to analyze up to 35 variables simultaneously. The processing speed for the ALGO Group was 270 images/0.14 sec. The coefficients of determination (R²) for the variables analyzed by the conventional method and the algorithm were found to be considerably high (0.84–0.97, p < 0.001 for testicular parenchyma images; 0.97–0.99, p < 0.001 for pampiniform plexus). The high correlations indicate that the algorithm produces results consistent with the conventional method, demonstrating its reliability. The Pearson correlation coefficients between the conventional analyses and the algorithm were significant (0.92–0.98, p < 0.001 for testicular parenchyma images; 0.98–0.99, p < 0.001 for pampiniform plexus). In addition, Bland-Altman concordance analyses showed that most points fell within the 95 % confidence interval for both techniques in the organs evaluated. Given the strong correlations demonstrated, the reduced processing time, and the reliability of the results, it can be concluded that this algorithmic approach can effectively replace conventional methods for assessing vascular function.

彩色多普勒超声是研究各器官组织灌注的有效方法，但目前的分析方法主观且耗时。本研究旨在发展并验证一种分析公牛睾丸和大网膜丛彩色多普勒图像的算法。在这项研究中，我们选择了2304张彩色多普勒图像（1152张用于睾丸软组织和旁膝神经丛），这些图像通过传统方法（CON组）、使用Adobe Fireworks®和ImageJ®进行像素分离和计数，或使用Python 3.10版本（ALGO组）开发的算法进行分析，该算法可以设置为同时分析多达35个变量。ALGO组的处理速度为270张/0.14 秒。常规方法和算法分析的变量的决定系数（R²）相当高（睾丸实质图像0.84-0.97,p <； 0.001；pampiniform丛0.97-0.99,p <； 0.001）。高相关性表明该算法得到的结果与传统方法一致，证明了该算法的可靠性。常规分析与算法之间的Pearson相关系数显著（睾丸实质图像0.92-0.98,p <； 0.001；旁膝神经丛0.98-0.99,p <； 0.001）。此外，Bland-Altman一致性分析显示，在评估的器官中，两种技术的大多数点都落在95% %的置信区间内。鉴于所证明的强相关性，减少的处理时间和结果的可靠性，可以得出结论，该算法方法可以有效地取代评估血管功能的传统方法。

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引用次数: 0

Effects of Zinc oxide nanoparticles on llama sperm cryopreservation 氧化锌纳米颗粒对羊驼精子冷冻保存的影响。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-09-15 DOI: 10.1016/j.anireprosci.2025.107994

Andrea Romina di Fonzo , Mariana Lucía Bertuzzi , María Victoria Amusquibar , María Ignacia Carretero

Zinc oxide nanoparticles (ZnO-NPs) have been reported to enhance sperm cryopreservation in several species, but their effect on frozen llama semen remains unexplored. This study aims to evaluate the effect of ZnO-NPs on sperm parameters in frozen/thawed llama semen. Fourteen ejaculates, obtained from seven male llamas, were each divided into three equal aliquots. Each aliquot was diluted with 1. AndroMed® with 20 % egg yolk and no ZnO-NPs (AM-EY0, Control); 2. AM-EY supplemented with 50 μg/ml of ZnO-NPs (AM-EY50) and 3. AM-EY supplemented with 100 μg/ml of ZnO-NPs (AM-EY100). Freezing was performed using an automatic machine. Evaluations were carried out on raw semen, immediately after dilution (0 h), and following the freezing/thawing process. Additionally, frozen/thawed samples were incubated at 37 °C and assessed at 15, 90, and 180 min. Data were analysed using Friedman tests, conventional ANOVA, or split-plot design models. The results showed no significant differences in sperm motility patterns, live sperm with intact acrosomes, membrane function, lipid peroxidation, sperm morphology, or DNA integrity among frozen/thawed groups (P > 0.05). A trend toward higher sperm vigour was detected in frozen/thawed samples cryopreserved with 50 and 100 μg/ml of ZnO-NPs (P = 0.08). Moreover, ZnO-NPs did not enhance sperm survival during post-thaw incubation at 37 °C for up to 180 min. In conclusion, supplementation of the AM-EY extender with 50 or 100 μg/ml ZnO-NPs did not provide broad protection against cryodamage in llama sperm. Further studies testing a wider range of concentrations are needed to assess their potential benefits for sperm cryopreservation in this species.

据报道，氧化锌纳米颗粒（ZnO-NPs）可以增强几个物种的精子冷冻保存，但它们对冷冻美洲驼精液的影响尚不清楚。本研究旨在评价ZnO-NPs对冷冻/解冻骆驼精液中精子参数的影响。从七只雄性大羊驼身上获得的十四次射精，每一次被分成三等份。每个等价物用1稀释。AndroMed®含20 %蛋黄，无ZnO-NPs （AM-EY0，对照）；2. 添加50 μg/ml ZnO-NPs (AM-EY50)；AM-EY中添加100 μg/ml的ZnO-NPs （AM-EY100）。冷冻是用自动机器进行的。在稀释后（0 h）和冷冻/解冻过程后立即对生精液进行评估。此外，冷冻/解冻的样品在37°C下孵育，并在15、90和180 min下进行评估。数据分析采用Friedman检验、传统方差分析或裂图设计模型。结果显示，冷冻/解冻组在精子运动模式、顶体完好活精子、膜功能、脂质过氧化、精子形态或DNA完整性方面均无显著差异（P > 0.05）。用50和100 μg/ml的ZnO-NPs冷冻/解冻后的精子活力有提高的趋势（P = 0.08）。此外，ZnO-NPs在37°C解冻后孵育长达180 min期间并没有提高精子存活率。综上所述，添加50或100 μg/ml ZnO-NPs的AM-EY扩展剂对骆驼精子的低温损伤没有广泛的保护作用。需要进一步的研究测试更大范围的浓度，以评估它们对该物种精子冷冻保存的潜在益处。

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引用次数: 0

Recombinant bovine somatotropin alters embryonic gene expression in superovulated ewes 重组牛促生长激素改变超排卵母羊胚胎基因表达。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-09-15 DOI: 10.1016/j.anireprosci.2025.107995

Ana Paula Pereira Schmidt , Pedro Henrique Nicolau Pinto , Juan Pedro Bottino González , Pedro Henrique de Mello Cotta Maia , Maria Paula da Costa Plaza , Brenda Barbosa Martins , Ribrio Ivan Tavares Pereira Batista , Joanna Maria G. Souza-Fabjan , Mário Felipe Alvarez Balaro , Jeferson Ferreira da Fonseca , Rodolfo Ungerfeld , Felipe Zandonadi Brandão

The aim of this study was to determine if recombinant bovine somatotropin (rbST) administration before a superovulatory protocol increases the ovulation rate and embryo quality, including gene expression, in sheep. Forty multiparous Santa Inês ewes were superovulated using 133 IU of FSH in six decreasing doses. Ewes from the treatment group (GST; n = 20), received a subcutaneous injection of 50 mg rbST 3 days before beginning the superovulatory treatment, while the control group (GCON; n = 20) remained as controls receiving saline solution. The ovaries were scanned with ultrasound to evaluate the follicular population, ovulation rate, and corpora lutea. Blood samples were collected to determine insulin-like growth factor 1 (IGF-1) concentrations. Embryos were recovered and analyzed for gene expression. The administration of rbST significantly increased serum IGF-1 levels while downregulating the expression of BCL2 and PRDX1 in embryos. Additionally, the expression of SIRT2 and CDH1 was upregulated in embryos collected from GST ewes, suggesting enhanced metabolic regulation and cell adhesion processes within embryonic cells. However, no significant differences were observed between GST and GCON in corpora lutea count, viable embryos. The treatment did not modify the follicular populations, the incidence of estrus, or the ovulation rate. In summary, administering a single dose of rbST before beginning the superovulation (SOV) treatment modulated the expression of genes related to embryo development. These findings provide novel insights into the non-ovarian effects of rbST in sheep and suggest a potential strategy to improve embryo developmental competence in assisted reproduction protocols.

本研究的目的是确定在超排卵方案之前给药重组牛生长激素（rbST）是否会增加绵羊的排卵率和胚胎质量，包括基因表达。40只多产Santa Inês母羊使用133 IU FSH分6次递减剂量超排卵。治疗组（GST; n = 20）的母羊在开始超排卵治疗前3天皮下注射50 mg rbST，而对照组（GCON; n = 20）仍然作为对照组接受生理盐水溶液。用超声扫描卵巢以评估卵泡数量、排卵率和黄体。采集血样测定胰岛素样生长因子1 （IGF-1）浓度。回收胚胎进行基因表达分析。rbST可显著提高胚胎血清IGF-1水平，同时下调BCL2和PRDX1的表达。此外，从GST母羊收集的胚胎中，SIRT2和CDH1的表达上调，表明胚胎细胞内的代谢调节和细胞粘附过程增强。然而，GST和GCON在黄体数量、活胚数量等方面无显著差异。治疗没有改变卵泡群、发情发生率或排卵率。总之，在开始超排卵（SOV）治疗前给予单剂量rbST可调节与胚胎发育相关的基因表达。这些发现为rbST对绵羊的非卵巢影响提供了新的见解，并提出了在辅助生殖方案中提高胚胎发育能力的潜在策略。

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引用次数: 0

Herbicide exposure impairs the morphofunctional parameters of bovine sperm 除草剂暴露会损害牛精子的形态功能参数。

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science

Pub Date : 2025-09-13 DOI: 10.1016/j.anireprosci.2025.107993

Diogo Ferreira Bicca , Rafaela Dalmolin Menezes , Luiza Gazeta Passos , Clara de Carvalho Silva , Mariana Gimenez dos Santos , Laura Rohde Brondani , Rogério Ferreira , Fábio Gallas Leivas , Daniela dos Santos Brum , Francielli Weber Santos Cibin

Glyphosate (GLP), 2,4-dichlorophenoxyacetic acid (2,4-D), and atrazine (ATZ) are the most commercialized herbicides in Brazil. Despite the damage to male fertility caused by pesticides, information on cattle remains limited. We evaluated the effects of exposing bovine sperm to concentrations of GLP, 2, 4-D, and ATZ. A semen pool from four bulls was incubated at 37°C for 1 and 3 h in TALP-Fert medium, and treated as follows: Control group (vehicle dimethyl sulfoxide); GLP at 5 (G5), 36 (G36), and 50 (G50) µg/mL; 2,4-D, at 0.5 (D05), 1 (D1), and 5 (D5) µM; and ATZ, at 0.05 (A005), 0.1 (A01), and 1 (A1) µM. Herbicide groups affected various kinematic parameters. Total motility was reduced by ATZ, while progressive motility decreased in all treatments compared to the control. Velocity-related kinematic parameters were significantly impaired by GLP treatment, and hyperactivity was negatively influenced by all three herbicide groups. Sperm morphology was altered in the G50, D5, and ATZ groups, with a higher incidence of major defects compared to the control. The hypo-osmotic swelling test revealed that plasma membrane integrity was compromised only in the A005 and A01 treatment groups. Regarding oxidative stress markers, although no differences were observed in reactive oxygen species generation or lipid peroxidation, total antioxidant capacity was significantly reduced by the 2,4-D treatment. Moreover, fertilization rates declined in the D05 group, which corresponded to the lowest 2,4-D concentration. These findings demonstrate that herbicide concentrations can adversely affect bovine spermatozoa by impairing critical quality parameters, ultimately compromising sperm function.

草甘膦（GLP）、2,4-二氯苯氧乙酸（2,4- d）和阿特拉津（ATZ）是巴西商业化程度最高的除草剂。尽管农药对男性生育能力造成了损害，但关于牛的信息仍然有限。我们评估了牛精子暴露于GLP、2,4 - d和ATZ浓度的影响。选取4头公牛的精液池，在TALP-Fert培养基中于37°C孵育1和3 h，处理如下：对照组（二甲基亚砜）；GLP浓度为5 （G5）、36 （G36）、50 （G50）µg/mL；2,4- d, 0.5 (D05), 1 (D1), 5 （D5）µM；和ATZ分别为0.05 （A005）、0.1 （A01）和1 （A1）µM。除草剂基团对各运动参数有影响。与对照组相比，ATZ降低了总运动性，而所有治疗的进行性运动性均下降。GLP处理显著损害了与速度相关的运动学参数，三种除草剂组均对多动性产生负面影响。G50、D5和ATZ组的精子形态发生了改变，与对照组相比，主要缺陷的发生率更高。低渗透肿胀试验显示，只有A005和A01处理组的质膜完整性受到损害。在氧化应激标志物方面，尽管在活性氧生成和脂质过氧化方面没有观察到差异，但2,4- d处理显著降低了总抗氧化能力。D05组受精率下降，对应于最低的2,4- d浓度。这些发现表明，除草剂浓度可以通过损害关键质量参数对牛精子产生不利影响，最终损害精子功能。

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引用次数: 0