Applied Soil Ecology最新文献_第2页

Ammonia-oxidizing microorganisms overwhelm denitrifiers in determining the efficacy of DMPP in upland soils

IF 4.8 2区农林科学 Q1 SOIL SCIENCE

Applied Soil Ecology

Pub Date : 2025-02-16 DOI: 10.1016/j.apsoil.2025.105955

Jilin Lei , Donghan Yang , Yingying Sun , Saihong Wang , Qianyi Fan , Junhui Yin , Rui Liu , Qing Chen

The use of nitrification inhibitors (NIs) like 3,4-dimethylpyrazole phosphate (DMPP) in agricultural systems can effectively reduce nitrous oxide (N₂O) emissions and nitrate (NO₃⁻) leaching, but their effectiveness varies across different soils. This microcosm experiment evaluated the efficacy of DMPP and its impact on soil microbial communities in four upland soils in Northern China (Tianshui: TS, Shihezi: SHZ, Heze: HZ and Daxing: DX). Results indicated that DMPP exhibits varying inhibitory effects on N₂O emissions across different soils. The key microbes mediating N₂O emissions, particularly ammonia-oxidizing bacteria (AOB), are the primary contributors to this variability. Specifically, DMPP led to a substantial reduction in N₂O emissions in TS soil, inhibiting 81.3 % of emissions by suppressing both ammonia-oxidizing bacteria (AOB) and archaea (AOA). In SHZ soil, a 56.5 % reduction was observed, primarily attributed to decreased AOB amoA abundance. DX soil exhibited a 48.6 % reduction, linked to decreased AOA amoA abundance and an increase in nosZ-N₂O reducers. Conversely, HZ soil showed the lowest reduction at 27.7 %, where DMPP stimulated the abundance of nirS-type denitrifiers while inhibiting unclassified Nitrosomonadales, the dominant AOB genus, which correlated positively with the net nitrification rate. Additionally, DMPP positively influenced norank Crenarchaeota-AOA in TS soil, and Bradyrhizobium-nosZ and Saccharothrix-narG in HZ soil, all negatively associated with N₂O emissions. Soil properties such as total nitrogen, organic matter (SOM), ammonium (NH₄⁺), pH, and available phosphorus (AP) levels significantly shaped microbial responses to DMPP. These findings underscore the importance of soil-specific characteristics in optimizing DMPP application strategies for reducing N₂O emissions in upland soils.

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引用次数: 0

Potassium organomineral fertilizer alters the microbiome of a sandy loam tropical soil

IF 4.8 2区农林科学 Q1 SOIL SCIENCE

Applied Soil Ecology

Pub Date : 2025-02-16 DOI: 10.1016/j.apsoil.2025.105960

Caroline Figueiredo Oliveira , Lucas William Mendes , Luís Reynaldo Ferracciú Alleoni

Soil fertility depends on a series of physical, chemical, and biological factors that interact to create an environment favorable to plant growth. In conditions of low fertility, mineral and organic fertilizers are commonly used in agricultural systems. However, studies on the impact of these fertilizers on the soil microbiota are limited, particularly those focusing on fertilizers containing potassium, a crucial plant macronutrient. In this study, we evaluated how potassium organomineral fertilizer (OMF) influences soil bacterial and fungal communities compared to potassium chloride (KCl), a conventional agricultural source in the humid tropics. Both the conventional particle size and the ground form used to produce OMF were examined. Samples of a sandy loam Typic Hapludox were incubated with the fertilizers, and the bacterial and fungal communities were assessed through sequencing of the 16S rRNA and ITS regions, respectively. OMF reduced the richness and diversity of bacterial and fungal communities, and this effect was attributed to the nutrient composition of OMF, rich in calcium and magnesium, which were absent in other treatments. Different fertilizers selected specific bacterial and fungal phyla, demonstrating their ability to influence community structure. Notably, OMF favored Proteobacteria and Bacteroidota, while KCl increased the abundance of Actinobacteriota and Firmicutes. The granulometry of KCl also influenced the soil microbial community, with smaller granules having greater soil contact, thus affecting chemical conditions and microbial composition. The OMF treatment enriched several bacterial genera, including Microvirga, Phenylobacterium, and Azospirillum, while increasing only the fungal genus Ascobolus. While OMF application reduced microbial richness and diversity, it favored specific microbial groups beneficial for agriculture, such as those involved in organic compound degradation and nitrogen cycling. These compositional changes may have significant implications for nutrient cycling and soil organic matter decomposition, highlighting the need for further studies to understand the underlying mechanisms and broader ecological impacts.

{"title":"Potassium organomineral fertilizer alters the microbiome of a sandy loam tropical soil","authors":"Caroline Figueiredo Oliveira , Lucas William Mendes , Luís Reynaldo Ferracciú Alleoni","doi":"10.1016/j.apsoil.2025.105960","DOIUrl":"10.1016/j.apsoil.2025.105960","url":null,"abstract":"<div><div>Soil fertility depends on a series of physical, chemical, and biological factors that interact to create an environment favorable to plant growth. In conditions of low fertility, mineral and organic fertilizers are commonly used in agricultural systems. However, studies on the impact of these fertilizers on the soil microbiota are limited, particularly those focusing on fertilizers containing potassium, a crucial plant macronutrient. In this study, we evaluated how potassium organomineral fertilizer (OMF) influences soil bacterial and fungal communities compared to potassium chloride (KCl), a conventional agricultural source in the humid tropics. Both the conventional particle size and the ground form used to produce OMF were examined. Samples of a sandy loam Typic Hapludox were incubated with the fertilizers, and the bacterial and fungal communities were assessed through sequencing of the 16S rRNA and ITS regions, respectively. OMF reduced the richness and diversity of bacterial and fungal communities, and this effect was attributed to the nutrient composition of OMF, rich in calcium and magnesium, which were absent in other treatments. Different fertilizers selected specific bacterial and fungal phyla, demonstrating their ability to influence community structure. Notably, OMF favored Proteobacteria and Bacteroidota, while KCl increased the abundance of Actinobacteriota and Firmicutes. The granulometry of KCl also influenced the soil microbial community, with smaller granules having greater soil contact, thus affecting chemical conditions and microbial composition. The OMF treatment enriched several bacterial genera, including <em>Microvirga</em>, <em>Phenylobacterium</em>, and <em>Azospirillum</em>, while increasing only the fungal genus <em>Ascobolus</em>. While OMF application reduced microbial richness and diversity, it favored specific microbial groups beneficial for agriculture, such as those involved in organic compound degradation and nitrogen cycling. These compositional changes may have significant implications for nutrient cycling and soil organic matter decomposition, highlighting the need for further studies to understand the underlying mechanisms and broader ecological impacts.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"207 ","pages":"Article 105960"},"PeriodicalIF":4.8,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impacts of shrub branch shelter and nitrogen addition on soil microbial activity and plant litter decomposition in a desert steppe

IF 4.8 2区农林科学 Q1 SOIL SCIENCE

Applied Soil Ecology

Pub Date : 2025-02-16 DOI: 10.1016/j.apsoil.2025.105956

Xiongkui Lin , Rebecca L. Schneider , Stephen J. Morreale , Hongmei Wang , Jianping Li , Zhigang Li

Shrub encroachment and nitrogen (N) deposition have become two critical factors that cause global grassland ecosystems degradation. However, the combined effects of shrub branch shelter and N deposition on soil microbial activity and litter decomposition in grasslands remain obscure. This study adopted four branch shelter levels of 0 % (no shelter), 30 %, 50 % and 70 % as the main plots, and four N additions including 0 g m⁻² a⁻¹, 10 g m⁻² a⁻¹, 20 g m⁻² a⁻¹ and 40 g m⁻² a⁻¹ as subplots in a Northwest China desert steppe. Subsequently, soil temperature, soil moisture, pH, N content, activities of C and N cycling-related enzymes, and microbial community structure between 0 and 5 cm were determined post 2 years, and litter decomposition from dominant plants was also determined. The results demonstrated that N additions increased the soil inorganic N content, but decreased soil pH value. However, branch shelters enhanced soil moisture, yet had no effect on soil pH value and N content. As results, N additions inhibited soil N cycling-related enzyme activities, but branch shelters accelerated the activities of the most tested extracellular enzymes. Branch shelters also presented opposite potentials to N additions in affecting soil microbial community structure. Overall, shrub shelters counteracted the negative effects of nitrogen deposition on soil enzyme activity and microbial community structure. Consequently, both branch shelter and N addition promoted litter decomposition. Therefore, branch shelter could be used as feasible measures to restore degraded grasslands caused by shrub encroachment and N deposition in arid and semiarid areas.

{"title":"The impacts of shrub branch shelter and nitrogen addition on soil microbial activity and plant litter decomposition in a desert steppe","authors":"Xiongkui Lin , Rebecca L. Schneider , Stephen J. Morreale , Hongmei Wang , Jianping Li , Zhigang Li","doi":"10.1016/j.apsoil.2025.105956","DOIUrl":"10.1016/j.apsoil.2025.105956","url":null,"abstract":"<div><div>Shrub encroachment and nitrogen (N) deposition have become two critical factors that cause global grassland ecosystems degradation. However, the combined effects of shrub branch shelter and N deposition on soil microbial activity and litter decomposition in grasslands remain obscure. This study adopted four branch shelter levels of 0 % (no shelter), 30 %, 50 % and 70 % as the main plots, and four N additions including 0 g m<sup>−2</sup> a<sup>−1</sup>, 10 g m<sup>−2</sup> a<sup>−1</sup>, 20 g m<sup>−2</sup> a<sup>−1</sup> and 40 g m<sup>−2</sup> a<sup>−1</sup> as subplots in a Northwest China desert steppe. Subsequently, soil temperature, soil moisture, pH, N content, activities of C and N cycling-related enzymes, and microbial community structure between 0 and 5 cm were determined post 2 years, and litter decomposition from dominant plants was also determined. The results demonstrated that N additions increased the soil inorganic N content, but decreased soil pH value. However, branch shelters enhanced soil moisture, yet had no effect on soil pH value and N content. As results, N additions inhibited soil N cycling-related enzyme activities, but branch shelters accelerated the activities of the most tested extracellular enzymes. Branch shelters also presented opposite potentials to N additions in affecting soil microbial community structure. Overall, shrub shelters counteracted the negative effects of nitrogen deposition on soil enzyme activity and microbial community structure. Consequently, both branch shelter and N addition promoted litter decomposition. Therefore, branch shelter could be used as feasible measures to restore degraded grasslands caused by shrub encroachment and N deposition in arid and semiarid areas.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"207 ","pages":"Article 105956"},"PeriodicalIF":4.8,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Landscape fragmentation impacts springtail community assembly in subtropical agroecosystems

IF 4.8 2区农林科学 Q1 SOIL SCIENCE

Applied Soil Ecology

Pub Date : 2025-02-16 DOI: 10.1016/j.apsoil.2025.105961

Douglas Alexandre , Luís Carlos Iuñes de Oliveira Filho , Dennis Goss-Souza , Ana Carolina Lovatel , Pâmela Niederauer Pompeo , Marcos Benedito Shimalski , Dilmar Baretta , Osmar Klauberg-Filho

This study investigated the impact of landscape fragmentation on springtail (Collembola) communities in subtropical regions. We collected soil core samples across landscapes with Low, Medium, and High fragmentation levels. Springtail morphotypes were identified and characterized using the Eco-Morphological Index (EMI), incorporating physical, chemical, and microbiological properties of the soil and litter. Our findings reveal a negative impact of fragmentation on springtail diversity. Landscapes with lower fragmentation exhibited higher beta diversity, indicating a more diverse and distinct assemblage of morphotypes. This pattern persisted across two sampling periods. Additionally, these landscapes shared a greater proportion of morphotypes with those of intermediate fragmentation compared to highly fragmented areas, highlighting the importance of habitat connectivity. Specialist morphotypes, which occur in less than one-third of the samples within a specific habitat type, were more prevalent in less fragmented areas, suggesting their vulnerability to habitat disturbances associated with fragmentation. Notably, specialist morphotypes (Ed1 and Ed8) were found exclusively in less fragmented landscapes during the first sampling. In addition, our findings reveal a positive correlation between geographic distance and community dissimilarity at the regional scale, with the highest beta diversity observed in the least fragmented landscape. Overall, this study provides compelling evidence that landscape fragmentation negatively impacts springtail communities, affecting their diversity, structure, and niche occupancy. These findings emphasize the importance of considering fragmentation in conservation practices to protect springtail biodiversity and maintain healthy ecosystems.

{"title":"Landscape fragmentation impacts springtail community assembly in subtropical agroecosystems","authors":"Douglas Alexandre , Luís Carlos Iuñes de Oliveira Filho , Dennis Goss-Souza , Ana Carolina Lovatel , Pâmela Niederauer Pompeo , Marcos Benedito Shimalski , Dilmar Baretta , Osmar Klauberg-Filho","doi":"10.1016/j.apsoil.2025.105961","DOIUrl":"10.1016/j.apsoil.2025.105961","url":null,"abstract":"<div><div>This study investigated the impact of landscape fragmentation on springtail (Collembola) communities in subtropical regions. We collected soil core samples across landscapes with Low, Medium, and High fragmentation levels. Springtail morphotypes were identified and characterized using the Eco-Morphological Index (EMI), incorporating physical, chemical, and microbiological properties of the soil and litter. Our findings reveal a negative impact of fragmentation on springtail diversity. Landscapes with lower fragmentation exhibited higher beta diversity, indicating a more diverse and distinct assemblage of morphotypes. This pattern persisted across two sampling periods. Additionally, these landscapes shared a greater proportion of morphotypes with those of intermediate fragmentation compared to highly fragmented areas, highlighting the importance of habitat connectivity. Specialist morphotypes, which occur in less than one-third of the samples within a specific habitat type, were more prevalent in less fragmented areas, suggesting their vulnerability to habitat disturbances associated with fragmentation. Notably, specialist morphotypes (Ed1 and Ed8) were found exclusively in less fragmented landscapes during the first sampling. In addition, our findings reveal a positive correlation between geographic distance and community dissimilarity at the regional scale, with the highest beta diversity observed in the least fragmented landscape. Overall, this study provides compelling evidence that landscape fragmentation negatively impacts springtail communities, affecting their diversity, structure, and niche occupancy. These findings emphasize the importance of considering fragmentation in conservation practices to protect springtail biodiversity and maintain healthy ecosystems.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"207 ","pages":"Article 105961"},"PeriodicalIF":4.8,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comammox Nitrospira and ammonia-oxidizing bacteria are metabolically active in a subtropical estuarine wetland

IF 4.8 2区农林科学 Q1 SOIL SCIENCE

Applied Soil Ecology

Pub Date : 2025-02-14 DOI: 10.1016/j.apsoil.2025.105964

Mengmeng Feng , Yongxin Lin , Guiping Ye , Zi-Yang He , Dong Zhu , Hang-Wei Hu , Yuheng Cheng , Fengyi Han , Ping Yang , Ji-Zheng He

Plant species profoundly influence soil microorganisms, yet their impact on active ammonia-oxidizing microorganisms remains largely unclear in subtropical estuarine wetlands. Here, we employed DNA stable isotope probing (DNA-SIP) technique to identify the active ammonia oxidizers under two typical plant species, Phragmites australis and Spartina alterniflora, as well as on a bare tidal flat in the Min River estuary. Our results revealed that comammox Nitrospira and ammonia-oxidizing bacteria (AOB) rather than archaea (AOA) were metabolically active in the P. australis and bare tidal flat soils. However, in S. alterniflora soils, the activity of ammonia oxidizers was inhibited, highlighting the critical role of plant species in shaping their community. The active ammonia oxidizers were primarily dominated by Nitrosomonas for AOB and clade A.1 for comammox Nitrospira. In comparison, bare tidal flat soils had a lower proportion of Nitrosospira and a higher relative abundance of comammox Nitrospira clade A.2 than those associated with P. australis. Taken together, our findings emphasize the importance of AOB and comammox Nitrospira, rather than AOA, in the nitrification processes within coastal wetlands, and underscore the critical role of plant species as a mediator.

{"title":"Comammox Nitrospira and ammonia-oxidizing bacteria are metabolically active in a subtropical estuarine wetland","authors":"Mengmeng Feng , Yongxin Lin , Guiping Ye , Zi-Yang He , Dong Zhu , Hang-Wei Hu , Yuheng Cheng , Fengyi Han , Ping Yang , Ji-Zheng He","doi":"10.1016/j.apsoil.2025.105964","DOIUrl":"10.1016/j.apsoil.2025.105964","url":null,"abstract":"<div><div>Plant species profoundly influence soil microorganisms, yet their impact on active ammonia-oxidizing microorganisms remains largely unclear in subtropical estuarine wetlands. Here, we employed DNA stable isotope probing (DNA-SIP) technique to identify the active ammonia oxidizers under two typical plant species, <em>Phragmites australis</em> and <em>Spartina alterniflora</em>, as well as on a bare tidal flat in the Min River estuary. Our results revealed that comammox <em>Nitrospira</em> and ammonia-oxidizing bacteria (AOB) rather than archaea (AOA) were metabolically active in the <em>P. australis</em> and bare tidal flat soils. However, in <em>S. alterniflora</em> soils, the activity of ammonia oxidizers was inhibited, highlighting the critical role of plant species in shaping their community. The active ammonia oxidizers were primarily dominated by <em>Nitrosomonas</em> for AOB and clade A.1 for comammox <em>Nitrospira</em>. In comparison, bare tidal flat soils had a lower proportion of <em>Nitrosospira</em> and a higher relative abundance of comammox <em>Nitrospira</em> clade A.2 than those associated with <em>P. australis</em>. Taken together, our findings emphasize the importance of AOB and comammox <em>Nitrospira</em>, rather than AOA, in the nitrification processes within coastal wetlands, and underscore the critical role of plant species as a mediator.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"207 ","pages":"Article 105964"},"PeriodicalIF":4.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the plant growth promotion and the antifungal potency against maize pathogen Fusarium verticillioides by desert-derived endophytes Bacillus subtilis RA15 and Bacillus tequilensis FC6 and their lipopeptides

IF 4.8 2区农林科学 Q1 SOIL SCIENCE

Applied Soil Ecology

Pub Date : 2025-02-14 DOI: 10.1016/j.apsoil.2025.105949

Vanessa Nya Dinango , Hanen Dhouib , Lobna Jlail , Slim Tounsi , Fabrice Fekam Boyom , Louise Nana Wakam , Olfa Frikha-Gargouri

This study explores the potential of two novel bacteria Bacillus subtilis RA15 and Bacillus tequilensis FC6 screened in vivo for their ability to protect maize plants against Fusarium verticillioides and to enhance plant growth. Both bacterial strains demonstrated significant growth inhibition of F. verticillioides in vitro, as evidenced by inhibition zones and mycelial morphology changes. Additionally, cyclic lipopeptides, including iturin and fengycin, known for their antimicrobial properties, were detected in purified extracts from cell-free supernatants of strain cultures and from treated maize plants. Interestingly, in vivo experiments on maize seeds using lipopeptide extracts at the lower concentration tested (10 mg/L), especially from FC6 not only provided significant protection against the disease comparable to that of the chemical control (55 % and 51 % for strain FC6 and the control, respectively), but also significantly improved maize shoot and root lengths (660 % and 191 %) and weights (371 % and 205 %, respectively). These findings suggest a direct relationship between the produced lipopeptides and the biocontrol and growth promotion capacity of RA15 and FC6 strains. Overall, B. subtilis RA15, B. tequilensis FC6, and their antifungal compounds hold great promise as biocontrol agents for managing Fusarium ear and root rot disease.

{"title":"Unveiling the plant growth promotion and the antifungal potency against maize pathogen Fusarium verticillioides by desert-derived endophytes Bacillus subtilis RA15 and Bacillus tequilensis FC6 and their lipopeptides","authors":"Vanessa Nya Dinango , Hanen Dhouib , Lobna Jlail , Slim Tounsi , Fabrice Fekam Boyom , Louise Nana Wakam , Olfa Frikha-Gargouri","doi":"10.1016/j.apsoil.2025.105949","DOIUrl":"10.1016/j.apsoil.2025.105949","url":null,"abstract":"<div><div>This study explores the potential of two novel bacteria <em>Bacillus subtilis</em> RA15 and <em>Bacillus tequilensis</em> FC6 screened <em>in vivo</em> for their ability to protect maize plants against <em>Fusarium verticillioides</em> and to enhance plant growth. Both bacterial strains demonstrated significant growth inhibition of <em>F. verticillioides in vitro,</em> as evidenced by inhibition zones and mycelial morphology changes. Additionally, cyclic lipopeptides, including iturin and fengycin, known for their antimicrobial properties, were detected in purified extracts from cell-free supernatants of strain cultures and from treated maize plants. Interestingly, <em>in vivo</em> experiments on maize seeds using lipopeptide extracts at the lower concentration tested (10 mg/L), especially from FC6 not only provided significant protection against the disease comparable to that of the chemical control (55 % and 51 % for strain FC6 and the control, respectively), but also significantly improved maize shoot and root lengths (660 % and 191 %) and weights (371 % and 205 %, respectively). These findings suggest a direct relationship between the produced lipopeptides and the biocontrol and growth promotion capacity of RA15 and FC6 strains. Overall, <em>B. subtilis</em> RA15, <em>B. tequilensis</em> FC6, and their antifungal compounds hold great promise as biocontrol agents for managing Fusarium ear and root rot disease.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"207 ","pages":"Article 105949"},"PeriodicalIF":4.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soil nematode diversity depends on understory plant species richness in a subalpine forest

IF 4.8 2区农林科学 Q1 SOIL SCIENCE

Applied Soil Ecology

Pub Date : 2025-02-13 DOI: 10.1016/j.apsoil.2025.105957

Suhui Ma , Yangang Li , Fan Fan , Wenjing Fang , Qiong Cai , Xinyu Xiong , Zhiming Zhang , Jiangling Zhu , Chengjun Ji

As model soil invertebrates, nematodes are the most numerous and functionally diverse multicellular animals in terrestrial ecosystems, having significant effects on plant productivity, soil food web, and organic matter decomposition. While abiotic influences on soil nematode diversity are well known, the effects of biological factors and their interactions remain unclear. Based on combined field surveys with high-throughput sequencing, this study evaluated the impacts of biotic and abiotic factors on soil nematode species richness, Shannon diversity and trophic diversity in a subalpine coniferous forest in Sichuan, China. The results showed that plant diversity and richness exerted a greater influence on the species richness (ranging from 12 to 61) and Shannon index (ranging from 1.0 to 3.5) of soil nematode than abiotic factors (elevation and soil properties). Compared with trees, the species richness and density of understory shrubs and herbs exhibited significant negative correlations with soil nematode diversity. Interestingly, the relative abundances of lower trophic groups were mediated by both plants and higher trophic groups, whereas a bottom-up effect was observed on predators. These findings highlight the vital role of understory shrubs and herbs in shaping the trophic structure and diversity of soil nematode communities in coniferous forests, suggesting that understory diversity should be incorporated into models of soil nematode food webs in forest ecosystems.

{"title":"Soil nematode diversity depends on understory plant species richness in a subalpine forest","authors":"Suhui Ma , Yangang Li , Fan Fan , Wenjing Fang , Qiong Cai , Xinyu Xiong , Zhiming Zhang , Jiangling Zhu , Chengjun Ji","doi":"10.1016/j.apsoil.2025.105957","DOIUrl":"10.1016/j.apsoil.2025.105957","url":null,"abstract":"<div><div>As model soil invertebrates, nematodes are the most numerous and functionally diverse multicellular animals in terrestrial ecosystems, having significant effects on plant productivity, soil food web, and organic matter decomposition. While abiotic influences on soil nematode diversity are well known, the effects of biological factors and their interactions remain unclear. Based on combined field surveys with high-throughput sequencing, this study evaluated the impacts of biotic and abiotic factors on soil nematode species richness, Shannon diversity and trophic diversity in a subalpine coniferous forest in Sichuan, China. The results showed that plant diversity and richness exerted a greater influence on the species richness (ranging from 12 to 61) and Shannon index (ranging from 1.0 to 3.5) of soil nematode than abiotic factors (elevation and soil properties). Compared with trees, the species richness and density of understory shrubs and herbs exhibited significant negative correlations with soil nematode diversity. Interestingly, the relative abundances of lower trophic groups were mediated by both plants and higher trophic groups, whereas a bottom-up effect was observed on predators. These findings highlight the vital role of understory shrubs and herbs in shaping the trophic structure and diversity of soil nematode communities in coniferous forests, suggesting that understory diversity should be incorporated into models of soil nematode food webs in forest ecosystems.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"207 ","pages":"Article 105957"},"PeriodicalIF":4.8,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of rice production and soil carbon sequestration utilizing nitrogen-fixing cyanobacteria

IF 4.8 2区农林科学 Q1 SOIL SCIENCE

Applied Soil Ecology

Pub Date : 2025-02-13 DOI: 10.1016/j.apsoil.2025.105940

Sen Li , Weigen Huang , Chengrong Peng , Xiaoyan Jing , Jixian Ding , Tong Chen , Ruilin Huang , Han Hu , Jizhong Zhou , Jiabao Zhang , Yuting Liang

Farmland soils are currently experiencing severe degradation, with a significant decline in soil organic carbon (SOC) content. Nitrogen-fixing cyanobacteria, known for their efficient green manure properties, have considerable potential to improve soil quality. However, the underlying mechanisms driving their effects remain unclear. In this study, we utilized a nitrogen-fixing cyanobacterial strain (Anabaena azotica SJ-1), isolated from local Mollisol soil, to assess its impact on rice plant growth and to elucidate the associated mechanisms. The results indicated that Anabaena azotica SJ-1 significantly enhanced rice plant growth, particularly in low-yielding soils (dry weight of rice spikes increased by 38–74 % in high-yielding soils and 107–157 % in low-yielding soils). Soil pH, available nitrogen content, and activities of soil acid phosphatase and N-acetyl-β-glucosaminidase were all increased with the application of Anabaena azotica SJ-1. Additionally, SOC content increased, characterized by an increase in alkyl C and a decrease in amid/carbonyl C. Moreover, the metabolic activity of live microbes in the soil was enhanced. Genome sequencing revealed that Anabaena azotica SJ-1 has a genome consisting of 6,115,153 bp nucleotides, eight plasmids, and 5367 protein-coding genes. Carbohydrate metabolism was identified as the primary metabolic pathway, while energy metabolism relied primarily on oxidative phosphorylation. This study underscores the significant potential of nitrogen-fixing cyanobacteria to improve the quality and efficiency of degraded Mollisol soils.

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引用次数: 0

Promoting soil microbial community development in early primary succession on waste rock by mulching with ramial chipped wood, in a boreal context

IF 4.8 2区农林科学 Q1 SOIL SCIENCE

Applied Soil Ecology

Pub Date : 2025-02-13 DOI: 10.1016/j.apsoil.2025.105958

Simon Taurines , Armand Séguin , Marie Guittonny

The ecological restoration of mine waste rock corresponds to a context of primary succession, characterized by mineral substrates poor in organic matter and nutrients. The addition of organic matter, specifically in the form of ramial chipped wood (RCW) mulch, could facilitate the development of soil microbial communities crucial to ecosystem recovery on mineral substrates. This study examined the interaction between pioneer boreal tree species and soil microorganisms, exploring how a RCW mulch influences the development of microbial and plant communities in primary succession on different mineral substrates: waste rock and sand. The methodology of this research used an experimental design of four complete randomized blocks on an area composed of waste rock at the Lapa Mine, Quebec. Treatments (n = 4) included two mineral substrates (scarified waste rock or sand) with or without the addition of RCW. The study focused on seedlings of two tree species: Pinus banksiana and Betula papyrifera. Microbial community development was analyzed by metabarcoding, focusing on the rhizosphere of tree seedlings and bulk soil, five years after tree seedling establishment. After five years, RCW mulch boosted bacterial species richness and diversity, particularly around Pinus banksiana and on waste rock, although its effect on fungal diversity was less marked. RCW also favored the development of bacterial and fungal functional groups useful for plant growth. Microbial diversity was more influenced by the physicochemical properties of mineral substrates than by tree species, indicating a preponderant influence of the mineral substrate physicochemical properties during the very early microbial succession. By promoting beneficial bacterial diversity in pioneer trees, RCW appears to be a promising strategy for supporting ecological restoration in disturbed boreal environments.

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引用次数: 0

Soil depth and fertilizer shape fungal community composition in a long-term fertilizer agricultural field

IF 4.8 2区农林科学 Q1 SOIL SCIENCE

Applied Soil Ecology

Pub Date : 2025-02-12 DOI: 10.1016/j.apsoil.2025.105943

Yaqin Guo , Julien Guigue , Sara L. Bauke , Stefan Hempel , Matthias C. Rillig

Soil fungal communities are vital in agro-ecosystems, driving organic matter decomposition and nutrient cycling, yet their distribution across soil depths remain underexplored. This study utilized high-throughput sequencing of fungal ITS2 amplicons to investigate fungal richness, diversity, community composition, and potential functions along a depth gradient (0–100 cm) under various fertilizer treatments in the field (control, NK, NP, PK, NPK). Results revealed that fungal richness and diversity peaked in topsoil (0–30 cm) and markedly declined in subsoil layers (30–100 cm), with distinct fungal taxa present in each layer. The C to N ratio (C/N) (12.9 %) was the most important predictor for ASV observed richness, while Depth (12.8 %) and C/N (11.1 %) were the top predictors for Shannon diversity. Soil depth explained 17.0 % of the variation in community composition, while fertilizer treatments accounted for 8.4 %. Fertilization significantly altered fungal community composition in subsoil layers but had a minimal impact on topsoil communities, with unique biomarkers associated with each treatment. Soil properties, including total organic carbon, pH, electrical conductivity, C to N ratio, clay content, and bulk density, were significant factors driving fungal composition variation across depths. These findings underscore the importance of considering soil depth in studying the impact of fertilization on soil microbiota, providing valuable insights into the complex dynamics of soil microbial communities in response to long-term chemical fertilizer treatment. In the long term, greater insights into fungal dynamics across soil profiles can inform new strategies to help safeguard sustainability of soil, a critical resource for food security.

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引用次数: 0