Aquaculture Reports最新文献_第5页

Biofloc-based bacterial community forms an ecological barrier for resisting Vibrio parahaemolyticus infection in shrimp 以生物絮团为基础的细菌群落形成了对虾抗副溶血性弧菌感染的生态屏障

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2026-01-06 DOI: 10.1016/j.aqrep.2025.103329

Jing Wang , Fan Gao , Jiayun Yao , Lei Huang , Xuezhi Fu , Jianfeng Tang , Demin Zhang , Haipeng Guo

Biofloc system (BFS) is a practical method to improve shrimp health via manipulating microbial community. However, the contribution of bacterial communities regulated by BFS in enhancing disease resistance is currently unclear. Here, a cross-combination experiment was conducted using rearing water and shrimp from control and BFS to elucidate the roles of bacterial communities in resisting Vibrio parahaemolyticus infection. The results showed that shrimp cultured in BFS were more resistant to V. parahaemolyticus, and the combinations of shrimp from control (CS) with rearing water from BFS (BW), and shrimp from BFS (BS) with rearing water from control (CW) could all significantly increase the shrimp resistance to V. parahaemolyticus, compared to CW+CS. Following infection, the gut bacterial community in BW+CS and BW+BS groups exhibited less variation in similarity, and higher relative abundances of taxa from Rhodobacteraceae. SourceTracker analysis suggested that shrimp cultured with BW had a reduced contribution of Vibrionaceae from rearing water to the gut. Strains corresponding to four key differential OTUs were isolated from BFS to establish two synthetic communities, which offered a protection to shrimp against V. parahaemolyticus infection. Our study showed that bacterial communities in BFS, especially in rearing water, were important for shrimp to resist pathogen.

生物絮团系统（Biofloc system， BFS）是一种通过控制微生物群落来改善对虾健康的实用方法。然而，受BFS调控的细菌群落在增强抗病性方面的作用目前尚不清楚。本研究采用对照和BFS养殖水和对虾进行交叉组合试验，探讨细菌群落在抗副溶血性弧菌感染中的作用。结果表明：BFS养殖对虾对副溶血性弧菌的抗性较强，对照对虾（CS）与BFS养殖水（BW）、BFS养殖水（BS）与对照养殖水（CW）配用均能显著提高对虾对副溶血性弧菌的抗性。感染后，BW+CS组和BW+BS组肠道细菌群落相似性变化较小，红杆菌科类群相对丰度较高。SourceTracker分析表明，添加了BW的养殖对虾减少了养殖水中弧菌科对肠道的贡献。从BFS中分离出4个关键差异otu对应的菌株，建立了2个合成菌群，对对虾抗副溶血性弧菌感染具有保护作用。本研究表明，饵料中特别是养殖水体中的细菌群落对对虾抵抗病原菌具有重要作用。

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引用次数: 0

Effects of dietary n-3HUFA levels on growth, antioxidant capacity, and ovarian development in Litopenaeus vannamei broodstock shrimp 饲料n-3HUFA水平对凡纳滨对虾生长、抗氧化能力和卵巢发育的影响

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2026-01-06 DOI: 10.1016/j.aqrep.2025.103317

Weibin Huang , Biao Li , Beiping Tan , Digen Qin , Zhiyue Li , Xintong Su , Yansheng Liu , Shuang Zhang , Shiwei Xie , Xiaohui Dong

The present study investigated the effects of dietary n-3 highly unsaturated fatty acids (n-3 HUFA) on the growth, antioxidant capacity, and ovarian development of female Litopenaeus vannamei broodstock. Five experimental diets with varying levels of n-3 HUFA (2.00 %, 2.50 %, 3.50 %, 5.00 %, and 7.00 %) were formulated and fed to female shrimp over a 10-week period. The growth performance, biochemical indices in the hepatopancreas, ovary, and serum, as well as antioxidant enzyme activities, were measured. The results indicated that moderate n-3 HUFA supplementation (3.50–5.00 %) significantly enhanced weight gain (WG), specific growth rate (SGR), and gonadosomatic index (GSI), while higher or lower levels of n-3 HUFA did not show significant improvement in these parameters. Antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), showed optimal activities in shrimp fed diets with 3.50–5.00 % n-3 HUFA. Furthermore, serum and ovarian biochemical indicators, such as total cholesterol (TC), triglycerides (TG), and glucose (Glu), were positively influenced by dietary n-3 HUFA. Hormonal analysis revealed that estradiol (E2) and vitellogenin (VTG) levels in the serum and ovaries were significantly higher in the 5.00 % n-3 HUFA group, supporting enhanced reproductive performance. We recommend an n-3 HUFA level of 5.28–5.48 % in feed, based on the SGR and GSI fitting curves.

本试验研究了饲料中添加n-3高不饱和脂肪酸（n-3 HUFA）对凡纳滨对虾（Litopenaeus vannamei）雌亲鱼生长、抗氧化能力和卵巢发育的影响。配制5种n-3 HUFA水平分别为2.00 %、2.50 %、3.50 %、5.00 %和7.00 %的试验饲料，饲喂10周。测定其生长性能、肝胰腺、卵巢、血清生化指标及抗氧化酶活性。结果表明，适量添加n-3 HUFA（3.50-5.00 %）可显著提高增重（WG）、特定生长率（SGR）和促性腺指数（GSI），而较高或较低水平的n-3 HUFA对这些参数没有显著改善。抗氧化酶，包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px），在饲粮中添加3.50-5.00 % n-3 HUFA时表现出最佳活性。此外，饲粮n-3 HUFA对血清和卵巢生化指标，如总胆固醇（TC）、甘油三酯（TG）和葡萄糖（Glu）均有积极影响。激素分析显示，血清和卵巢中的雌二醇（E2）和卵黄蛋白原（VTG）水平在5.00 % n-3 HUFA组显著升高，支持增强的生殖性能。根据SGR和GSI拟合曲线，推荐饲料中n-3 HUFA水平为5.28 ~ 5.48 %。

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引用次数: 0

Integrated biochemical profiling, comparative transcriptome and weighted gene co-expression network analysis to explore the response mechanism of global warming and ocean acidification to the stress of Sepia esculenta larvae 综合生化分析、比较转录组和加权基因共表达网络分析，探讨全球变暖和海洋酸化对褐皮鱼（Sepia esculenta）幼虫应激的响应机制

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2026-01-06 DOI: 10.1016/j.aqrep.2025.103347

Xueyu Zhu , Yanchun Wang , Yuwei Zhang , Xiaokai Bao , Deyuan Chang , Weijun Wang , Mengshuo Zhang , Xiumei Liu , Zan Li , Jianmin Yang

The Sepia esculenta has high economic value and nutritional value, and accounts for a high proportion of the catch of cephalopods in China 's coastal waters. Global warming and ocean acidification, as major environmental problems currently facing the world, have a serious negative influence on the survival and breeding of S. esculenta. Therefore, in the research, transcriptome sequencing and biochemical quantitative analysis were performed on the larvae of S. esculenta after high temperature, low pH and combined stress at different time points, and the differential expressed genes (DEGs) and response mechanisms were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that these DEGs were mainly involved in a large number of immune-related biological processes and signaling pathways, including Immune response、Phagocytosis、Regulation of DNA-templated transcription and Positive regulation of DNA-templated transcription. Then, we further explored the functional relationship between these DEGs by constructing weighted gene co-expression network and protein-protein interaction networks. We identified ten hub genes including HSP90AA1, ALDH1L1, VPS13A, MAPK8IP1 and KDM6A. These hub genes may play an important role in the face of high temperature, low pH and their combined stress at different times. Our findings not only elucidate the molecular response mechanisms of S. esculenta to environmental stress and delineate the key regulatory pathways underlying its adaptation, but also provide a theoretical foundation for advancing pond cultivation.

褐皮鱼具有很高的经济价值和营养价值，在中国沿海水域的头足类捕捞中占有很高的比例。全球变暖和海洋酸化是当今世界面临的主要环境问题，对海参的生存和繁殖产生了严重的负面影响。因此，本研究通过对不同时间点的高温、低pH和联合胁迫下的棘球蚴幼虫进行转录组测序和生化定量分析，鉴定差异表达基因（DEGs）及其应答机制。基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析表明，这些deg主要参与大量免疫相关的生物学过程和信号通路，包括免疫应答、吞噬作用、dna模板转录调控和dna模板转录阳性调控。然后，我们通过构建加权基因共表达网络和蛋白-蛋白相互作用网络，进一步探索这些deg之间的功能关系。共鉴定出HSP90AA1、ALDH1L1、VPS13A、MAPK8IP1和KDM6A等10个枢纽基因。这些枢纽基因可能在不同时期面对高温、低pH及其联合胁迫时发挥重要作用。本研究结果不仅阐明了棘豆对环境胁迫的分子响应机制，揭示了棘豆适应环境胁迫的关键调控途径，而且为推进池塘养殖提供了理论基础。

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引用次数: 0

Temporal variability in greenhouse gas emissions from shrimp aquaculture ponds in China: A complete farming cycle assessment 中国对虾养殖池塘温室气体排放的时间变异性：一个完整的养殖周期评估

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2026-01-06 DOI: 10.1016/j.aqrep.2025.103356

Bowen Li , Chunxiu Chen , Xiaoqing Zhou , Huiru Liu , Zhixiong Zhou , Xiaoyu Wang , Jian Liang , Yongjun Guo , Shuang Liang

Extensive shrimp aquaculture can release a significant amount of greenhouse gas (GHG). Its rapid expansion in China has raised environmental concerns. Most studies focus on GHG emissions during the farming stage. They often overlook emissions that occur during non-farming seasons. This oversight has led to a limited understanding of the temporal variations in emissions throughout the complete farming cycles. This study analyzed CO₂, CH₄, and N₂O fluxes across different farming stages and identified key environmental drivers through a meta-analysis of 564 sets of data from 26 studies spanning 2012–2024. During the farming period, the fluxes of CO₂, CH₄ and N₂O from shrimp ponds were 8.05 mg m⁻² h⁻¹, 27.33 mg m⁻² h⁻¹ and 16.65 μg m⁻² h⁻¹. The non-farming period with average emissions of 14.53 mg m⁻² h⁻¹ for CO₂, 2.86 mg m⁻² h⁻¹ for CH₄, and 80.26 μg m⁻² h⁻¹ for N₂O. Water physicochemical parameters (like water temperature, pH, dissolved oxygen) primarily drove emissions during farming periods. In contrast, sediment characteristics (like water depth, sediment pH, sediment total carbon, sediment total nitrogen) became dominant during non-farming periods. Notably, excluding the non-farming period from assessments overestimated CH₄ emissions and underestimated CO₂ and N₂O emissions. These findings emphasize the necessity of year-round monitoring for accurate GHG emission assessments from shrimp aquaculture. They also provide crucial insights for developing effective climate mitigation strategies.

大规模的对虾养殖会释放大量的温室气体。该公司在中国的迅速扩张引发了对环境的担忧。大多数研究集中在农业阶段的温室气体排放。他们经常忽视非农业季节的排放。这种疏忽导致对整个农业周期中排放的时间变化的理解有限。本研究通过对2012-2024年间26项研究的564组数据的荟萃分析，分析了不同农业阶段的CO₂、CH₄和N₂O通量，并确定了关键的环境驱动因素。养殖期间，对虾池CO2、CH4和N2O通量分别为8.05 mg m−2 h−1、27.33 mg m−2 h−1和16.65 μ m−2 h−1。非农期CO2平均排放量为14.53 mg m−2 h−1，CH4平均排放量为2.86 mg m−2 h−1，N2O平均排放量为80.26 μ m−2 h−1。水的物理化学参数（如水温、pH值、溶解氧）主要驱动农业时期的排放。相反，沉积物特征（如水深、沉积物pH、沉积物总碳、沉积物总氮）在非农业时期占主导地位。值得注意的是，将非农业时期排除在评估之外，高估了CH4排放量，低估了CO2和N2O排放量。这些发现强调了全年监测对虾养殖温室气体排放准确评估的必要性。它们还为制定有效的减缓气候变化战略提供了至关重要的见解。

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引用次数: 0

Hepatic transcriptomic and lipidomic analyses reveal the responding mechanism of mandarin fish (Siniperca chuatsi) adapting to artificial feed in the early domestication periods 肝脏转录组学和脂质组学分析揭示了鳜鱼在驯化早期对人工饲料的适应机制

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2026-01-06 DOI: 10.1016/j.aqrep.2025.103316

Lele Fu , Zhimin Gu , Dayan Hu , Jinpeng Zhang , Cui Liu , Shu Wang , Tiantian Ye , Junjun Yan , Jilun Meng , Yutong Zheng , Rongfeng Wu , Xiaoming Zhu

This study aimed to elucidate the molecular mechanisms underlying hepatic adaptation in mandarin fish during early domestication by integrating transcriptomic and lipidomic analyses. Fish were fed either live prey (C) or artificial diets (F), and liver samples were collected on days 7 and 20 of the feeding trial to assess dynamic transcriptional and metabolic responses. No significant differences in body weight were observed between groups. Transcriptomic analysis revealed 861 differentially expressed genes on day 7 and 2922 on day 20, while lipidomic profiling identified 218 and 421 differentially abundant metabolites, respectively. Integrated multi-omics analysis revealed that steroid biosynthesis (particularly cholesterol synthesis), glycerophospholipid metabolism and sphingolipid signaling pathway were central to hepatic adaptation during domestication. Moreover, immune-related pathways, particularly the IL-17 signaling pathway and its downstream effectors (nfκb, tnfα, il-1β), were increasingly activated over time. In general, this study reveals continuous transcriptional and metabolic reprogramming in the liver during early domestication, providing insights into the molecular basis of dietary adaptation in mandarin fish. These findings lay a foundation for developing nutritionally optimized formulated feeds that support sustainable commercial production of mandarin fish.

本研究旨在通过整合转录组学和脂质组学分析，阐明鳜鱼在早期驯化过程中肝脏适应的分子机制。分别饲喂活饵料(C)和人工饵料(F)，并在饲喂试验的第7天和第20天采集肝脏样本，以评估动态转录和代谢反应。各组间体重无显著差异。转录组学分析显示，在第7天和第20天分别有861个和2922个差异表达基因，而脂质组学分析分别鉴定出218个和421个差异丰富的代谢物。综合多组学分析显示，在驯化过程中，类固醇生物合成（尤其是胆固醇合成）、甘油磷脂代谢和鞘脂信号通路是肝脏适应的核心。此外，随着时间的推移，免疫相关通路，特别是IL-17信号通路及其下游效应器（nfκb、tnfα、il-1β）被越来越多地激活。总的来说，本研究揭示了鳜鱼在早期驯化过程中肝脏中持续的转录和代谢重编程，为鳜鱼饮食适应的分子基础提供了新的见解。这些发现为开发营养优化的配方饲料，支持鳜鱼的可持续商业生产奠定了基础。

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引用次数: 0

Effects of replacing fish meal with a combination of corn protein concentrate and meat meal on growth performance, biochemical profile, digestive enzyme activity, antioxidant activity, immune response, and economical efficiency in juvenile black rockfish (Sebastes schlegelii) 玉米蛋白精与肉粉组合替代鱼粉对黑岩鱼幼鱼生长性能、生化指标、消化酶活性、抗氧化活性、免疫反应和经济效益的影响

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2026-01-06 DOI: 10.1016/j.aqrep.2025.103312

Tae Hoon Lee , Ki-Tae Kim , Hwa Yong Oh , Da Ye Kang , Young Wook Kim , Do Hyun Kwon , Bo Seong Gu , Hee Sung Kim

This study evaluated the effects of substituting fish meal (FM) with a 1:2 blend of corn protein concentrate and meat meal (CCM) on growth performance, digestive enzyme activity, biochemical composition, antioxidant and immune responses, and economic efficiency in juvenile black rockfish (Sebastes schlegelii). Seven experimental diets were prepared: a control diet containing 65 % FM and six test diets with 10 %, 20 %, 30 %, 40 %, 50 %, and 60 % of FM from the control diet progressively replaced with CCM. Juvenile rockfish (initial body weight: 3.6 ± 0.01 g) were randomly assigned to triplicate groups of 30 each and fed the experimental diet for 8 weeks. Growth parameters, including final body weight, weight gain, specific growth rate, feed efficiency, and protein efficiency ratio, significantly declined when FM replacement exceeded 40 %. Trypsin and lipase activity showed a linear decline with increasing CCM levels. Whole-body moisture content significantly increased, whereas crude protein content significantly decreased at 60 % FM replacement with CCM. Antioxidant enzyme activity and immune response markers declined at ≥ 50 % FM replacement. Although feed costs decreased with increasing CCM levels, economic conversion and profit indices were maximized at 40 % FM replacement. Broken-line regression analysis estimated the optimal FM-replacement level with CCM at 34.07 %. Overall, these findings suggest that up to 40 % of FM can be replaced with CCM in juvenile S. schlegelii diets without impairing growth, nutrient utilization, antioxidant function, or immune response, making it a cost-effective and sustainable alternative for aquafeeds.

本试验研究了玉米蛋白精与肉粉（CCM）以1:2的混合饲料替代鱼粉（FM）对黑岩鱼幼鱼生长性能、消化酶活性、生化组成、抗氧化和免疫反应以及经济效益的影响。配制了7种试验饲粮：一种是含有65% %鱼粉的对照饲粮，另一种是含有10% %、20% %、30 %、40 %、50 %和60 %鱼粉的试验饲粮，分别由对照饲粮逐步替换为CCM。选取初始体重为3.6 ± 0.01 g的岩鱼幼鱼，随机分为3个重复组，每组30只，饲喂试验饲料8周。当鱼粉替代量超过40 %时，末重、增重、特定生长率、饲料效率、蛋白质效率等生长参数显著下降。胰蛋白酶和脂肪酶活性随CCM水平的升高呈线性下降。用CCM替代60% %鱼粉显著提高了全鱼水分含量，而粗蛋白质含量显著降低。抗氧化酶活性和免疫反应指标在≥ 50 % FM替代时下降。饲料成本随饲料添加量的增加而降低，但在饲料替代量为40% %时，经济转化率和利润指标达到最大。折线回归分析估计CCM的最佳fm替代水平为34.07 %。总的来说，这些发现表明，高达40% %的鱼粉可以被石氏梭菌幼鱼饲料中的CCM替代，而不会影响其生长、营养利用、抗氧化功能或免疫反应，使其成为一种经济、可持续的水产饲料替代品。

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引用次数: 0

Ecophysiological evidence linking temperature variability and lion´s paw scallop Nodipecten subnodosus growth yields in the field 野外温度变化与狮子爪扇贝Nodipecten subnodosus生长产量相关的生态生理学证据

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2026-01-06 DOI: 10.1016/j.aqrep.2025.103330

Ilie S. Racotta, Natalia G. Joachin-Mejia, M. Teresa Sicard, Salvador E. Lluch-Cota

The present work examines the fitness of lion's paw scallops at several locations in connection to historical temperature variability, in order to inform the site selection for the cultivation of Nodipecten subnodosus. Temperature-dependent tolerance limits and energy budget were analyzed in laboratory and compared to environmental thermal variability at five sites along the Baja California peninsula, two on the Pacific coast (PC) and three on the Gulf of California coast (GC), and to an updated analysis of published growth information from culture experiences in these same sites. Scope for growth determinations indicate that the optimum temperature for N. subnodosus is 22ºC, whereas its higher temperature limit ranged between 28 and 31ºC, depending on acclimation temperature. Little differences between sites were observed for the proportion of time for which the temperature in the field coincides with the laboratory-derived optimal, whereas a clear distinction can be observed for the tolerance range, where high temperatures are only slightly attained at the PC sites (0 and 4 %), but ranges between 19 % and 30 % for the GC sites. The analysis of growth data from previous studies grossly coincides with higher growth rates at PC than at GC sites. Based on our observations we pose that thermal variability conditions along the PC should be preferred over the GC for the culture of this species, although site selection must also consider other environmental variables and dimensions, such as food availability, environment quality, culture methods, and socioeconomic aspects.

本研究考察了狮子爪扇贝在几个地点与历史温度变化的关系，以便为Nodipecten subnodosus的种植选址提供信息。在实验室中分析了温度依赖性耐受极限和能量收支，并将其与下加利福尼亚半岛5个地点的环境热变率进行了比较，其中2个地点位于太平洋沿岸（PC）， 3个地点位于加利福尼亚湾沿岸（GC），并对这些地点公布的生长信息进行了更新分析。生长范围测定表明，根据驯化温度的不同，nodosus的最适温度为22ºC，而最高温度限制在28 - 31ºC之间。现场温度与实验室推导的最佳温度相吻合的时间比例在不同地点之间差异不大，而在公差范围上可以观察到明显的区别，在PC地点只有轻微的高温（0和4 %），但在GC地点的范围在19 %和30 %之间。对以往研究中生长数据的分析大致一致表明，PC的生长速率高于GC。根据我们的观察，我们提出，对于该物种的培养，沿PC的热变异性条件应该优于GC，尽管选址还必须考虑其他环境变量和维度，如食物供应、环境质量、培养方法和社会经济方面。

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引用次数: 0

A moderate dietary potassium diformate (KDF) is favorable for the growth, health status of intestine of juvenile rice field eel, Monopterus albus 饲粮中添加适量的二甲酸钾（KDF）有利于黄鳝幼鱼的生长和肠道健康

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2026-01-06 DOI: 10.1016/j.aqrep.2026.103364

Jinping Wu , Lan Zhou , Jiali Jin, Huamei Yue, Jing Zhang, Huan Ye, Chuangju Li, Rui Ruan

To assess the effect of dietary potassium diformate (KDF) on intestine health of juvenile rice field eel, the biochemistry, histomorphology and microbiota properties of intestine were analyzed after an 8-week trial by feeding diets containing different levels of KDF (0.0 %, 0.3 %, 0.6 %, 0.9 %, 1.2 % and 1.5 % in the diets). The results indicated that specific growth rate (SGR) was not affected by the dietary KDF levels (P > 0.05). Fish fed diet with KDF level at 0.9 % presented a significant improvement on digestive enzymes activity of the foregut and stomach compared with control group (0 % KDF) (P < 0.05). The activities of intestine SOD, CAT, T-AOC and GSH-PX were significantly improved by the dietary KDF levels (P < 0.05). Intestine MDA content in the 1.2 % KDF group was significantly lower than that in the 0.3 %, 0.6 %, and 0.9 % treatments (P < 0.05). The significant elevation of AKP and ACP activities in intestine were observed in KDF supplementation groups (P < 0.05). The fish in 0.9 % KDF group showed significantly more foregut intestinal folds than that of the other treatments (P < 0.05). Inclusion of KDF in diets significantly increased the number of goblet cells in both the foregut and hindgut, and achieved the highest value at 0.9 % KDF group (P < 0.05). No significant effect was found on the α-diversity indices of gut (P > 0.05). At the phylum level, the intestinal microbiota was predominantly comprised of Proteobacteria, Firmicutes, Verrucomicrobiota, Fusobacteriota, Actinobacteriota, and Bacteroidota. The study revealed that an appropriate supplementation of KDF in diet is beneficial for the growth and intestinal health status of rice field eel by improving digestive enzymes activity, intestinal morphology and antioxidant capacity. The optimum supplementation level of KDF in juvenile rice field eel diet is determined at 0.88 g/kg based on SGR.

为评价饲粮中添加二甲酸钾（KDF）对稻田鳗鲡幼鱼肠道健康的影响，通过饲喂不同水平的KDF（0.0 %、0.3 %、0.6 %、0.9 %、1.2 %和1.5 %）的试验，分析了8周后稻田鳗鲡幼鱼肠道的生物化学、组织形态学和微生物群特性。结果表明，特定生长率（SGR）不受饲粮KDF水平的影响（P >； 0.05）。饲粮中KDF水平为0.9 %时，前肠和胃消化酶活性显著高于对照组（0 % KDF）（P <； 0.05）。饲粮KDF水平显著提高了肠道SOD、CAT、T-AOC和GSH-PX活性（P <； 0.05）。1.2 % KDF组肠道MDA含量显著低于0.3 %、0.6 %和0.9 %处理（P <； 0.05）。KDF添加组肠内AKP和ACP活性显著升高（P <； 0.05）。0.9% % KDF组鱼的前肠肠褶皱显著多于其他处理（P <； 0.05）。饲粮中添加KDF显著提高了前肠和后肠杯状细胞的数量，并在KDF含量为0.9 %时达到最高值（P <； 0.05）。对肠道α-多样性指标无显著影响（P >； 0.05）。在门水平上，肠道微生物群主要由变形菌门、厚壁菌门、疣菌门、梭菌门、放线菌门和拟杆菌门组成。由此可见，饲粮中适量添加KDF可改善稻田鳗鲡消化酶活性、肠道形态和抗氧化能力，有利于稻田鳗鲡生长和肠道健康。以SGR为基础，确定稻田鳗鲡幼鱼饲粮中KDF的适宜添加水平为0.88 g/kg。

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引用次数: 0

The role of gender in quantifying the influence of online marketing on the performance of fish marketers in Southwest, Nigeria 性别在量化在线营销对尼日利亚西南部鱼类营销人员绩效的影响中的作用

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2026-01-06 DOI: 10.1016/j.aqrep.2025.103326

Adewale Isaac Olutumise

The rapid development of online markets, especially amid the global COVID-19 pandemic, has signified a fundamental change in the dynamics of markets and economies. This holds implications for various sectors, including fisheries and aquaculture. Despite the growing body of literature acknowledging the significance of online marketing, there exists a notable gap in understanding how online marketing influences the performance of marketers from the perspective of gender. Therefore, this research seeks to bridge this gap by conducting an empirical study on the role of gender in quantifying the influence of online market participation on the performance of fish marketers. Cross-sectional data were collected through a well-designed questionnaire. A multistage sampling technique was used to select 240 respondents. A budgetary technique was employed in measuring the performance. The empirical framework of the study considered potential endogeneity between online marketing and performance by using an endogenous treatment effects model with a linear outcome. The findings revealed that, even though fish marketing is profitable in the area, females make more profit than their male counterparts. It was also recorded that ignoring potential endogeneity may affect the statistical significance of the online market participation estimate and the direction of the influence of online marketing on the performance of fish marketers. The study revealed that online marketing had a direct impact on the performance of female and male fish marketers, with marginal effects of 3.65 and 1.98, respectively. Variables such as education, access to smartphones, digital literacy, market size, and internet availability are significant determinants of online market participation in the area. Therefore, our findings support the literature that online marketing significantly increases performance. Internet infrastructure should be improved and made affordable to allow more users, and this could be designed according to the characteristics of different groups to promote the marketers’ livelihoods.

在线市场的快速发展，特别是在2019冠状病毒病全球大流行期间，标志着市场和经济动态的根本变化。这对包括渔业和水产养殖在内的各个部门都有影响。尽管越来越多的文献承认网络营销的重要性，但从性别的角度来理解网络营销如何影响营销人员的绩效，存在着显著的差距。因此，本研究试图通过对性别在量化在线市场参与对鱼类营销人员绩效的影响方面的作用进行实证研究来弥合这一差距。通过精心设计的问卷收集横截面数据。采用多阶段抽样技术，选取240名受访者。在衡量业绩时采用了预算技术。本研究的经验框架通过使用具有线性结果的内源性治疗效果模型考虑了在线营销与绩效之间的潜在内生性。调查结果显示，尽管该地区的鱼类营销是有利可图的，但雌性比雄性赚得更多。还记录到，忽略潜在的内生性可能会影响在线市场参与估计的统计显著性以及网络营销对鱼类营销人员绩效的影响方向。研究发现，网络营销对女性和男性鱼类营销人员的绩效有直接影响，边际效应分别为3.65和1.98。教育、智能手机普及率、数字素养、市场规模和互联网可用性等变量是该地区在线市场参与的重要决定因素。因此，我们的研究结果支持了网络营销显著提高绩效的文献。完善互联网基础设施，让更多的用户负担得起，这可以根据不同群体的特点进行设计，促进营销人员的生计。

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引用次数: 0

Experimental protocol for bath infection of Atlantic salmon with Tenacibaculum dicentrarchi: Aiming for target mortalities 大西洋大马哈鱼浴池感染双叉腱杆菌的实验方案：以目标死亡率为目标

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2026-01-05 DOI: 10.1016/j.aqrep.2025.103345

Almendra Benavides , Ruth Montero , Roque Scarpa , Marcos Mancilla , Margareth Øverland , Mónica Imarai

Developing experimental protocols to establish infection models in fish is essential for identifying new and effective preventive tools to improve fish health and welfare. Tenacibaculosis, caused by Tenacibaculum species, is a significant concern, particularly for Atlantic salmon (Salmo salar). However, replicable infection models for studying this disease remain limited. In this study, we developed a reliable bath infection method for T. dicentrarchi, enabling targeted, controlled mortality rates. First, using flow cytometry, we accurately quantified viable bacteria that were subsequently used for bath infection. A consistent mortality of ∼24 % was achieved across experiments, even when scaling up bath infection volumes. Infected fish displayed characteristic symptoms, including ulcers, oral erosions, and hemorrhages, and PCR confirmed bacterial presence. This protocol improved reproducibility in experimental infections with T. dicentrarchi, supporting research on new prophylactic methods and treatments against Tenacibaculosis.

制定实验方案以建立鱼类感染模型对于确定新的和有效的预防工具以改善鱼类健康和福利至关重要。由腱杆菌种类引起的腱杆菌病是一个重大问题，特别是对大西洋鲑鱼（Salmo salar）。然而，用于研究这种疾病的可复制感染模型仍然有限。在本研究中，我们开发了一种可靠的双氏弓形虫浴感染方法，实现了有针对性的、可控的死亡率。首先，使用流式细胞术，我们准确地定量了随后用于浴池感染的活菌。在所有实验中，即使扩大浴池感染量，死亡率也一致为~ 24 %。受感染的鱼表现出特有的症状，包括溃疡、口腔糜烂和出血，PCR证实了细菌的存在。该方案提高了双棘单胞杆菌实验感染的可重复性，支持了针对棘单胞杆菌病的新预防方法和治疗方法的研究。

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引用次数: 0