Aquaculture Reports最新文献_第5页

Phenotypic responses of the giant mussel Choromytilus chorus to prolonged upwelling conditions

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-02-16 DOI: 10.1016/j.aqrep.2025.102680

Sebastián I. Martel , Nicolás J. Leppes , Nelson A. Lagos , Cristian A. Vargas , Marco A. Lardies

Marine bivalve aquaculture is increasingly recognized as a sustainable alternative to mitigate potential animal protein shortages for human consumption. However, coastal aquaculture systems are susceptible to consequences of global change, including the projected increased duration of upwelling events within Eastern Boundary Upwelling Systems. These events can cause rapid environmental fluctuations that affect the biology of marine species including cultivable ones. This study investigates the phenotypic responses of the giant mussel Choromytilus chorus, an underutilized but promising shellfish species cultivated off the southern coast of Chile, to four laboratory-controlled combinations of temperature and pH conditions simulating the upwelling system they experience in farms. Our results show a remarkable capacity of this species to thrive in otherwise stressful environments. Notably, even under unprecedented warm and acidified conditions, juvenile individuals can effectively adjust their physiological state by elevating their rate of energy expenditure and increasing their feeding capacity. This plastic response allowed mussels to reduce the potentially negative consequences reported at an organismal level in other species, maintaining calcification and growth rates, and adjusting their periostracum's organic composition to an increased protein content under acidified conditions, thereby aiding shell production in a corrosive environment. Overall, our results underscore the potential for using suitably adapted cultivable species for food production in future changing scenarios.

{"title":"Phenotypic responses of the giant mussel Choromytilus chorus to prolonged upwelling conditions","authors":"Sebastián I. Martel , Nicolás J. Leppes , Nelson A. Lagos , Cristian A. Vargas , Marco A. Lardies","doi":"10.1016/j.aqrep.2025.102680","DOIUrl":"10.1016/j.aqrep.2025.102680","url":null,"abstract":"<div><div>Marine bivalve aquaculture is increasingly recognized as a sustainable alternative to mitigate potential animal protein shortages for human consumption. However, coastal aquaculture systems are susceptible to consequences of global change, including the projected increased duration of upwelling events within Eastern Boundary Upwelling Systems. These events can cause rapid environmental fluctuations that affect the biology of marine species including cultivable ones. This study investigates the phenotypic responses of the giant mussel <em>Choromytilus chorus</em>, an underutilized but promising shellfish species cultivated off the southern coast of Chile, to four laboratory-controlled combinations of temperature and pH conditions simulating the upwelling system they experience in farms. Our results show a remarkable capacity of this species to thrive in otherwise stressful environments. Notably, even under unprecedented warm and acidified conditions, juvenile individuals can effectively adjust their physiological state by elevating their rate of energy expenditure and increasing their feeding capacity. This plastic response allowed mussels to reduce the potentially negative consequences reported at an organismal level in other species, maintaining calcification and growth rates, and adjusting their periostracum's organic composition to an increased protein content under acidified conditions, thereby aiding shell production in a corrosive environment. Overall, our results underscore the potential for using suitably adapted cultivable species for food production in future changing scenarios.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102680"},"PeriodicalIF":3.2,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intensive cultivation of whiteleg shrimp (Litopenaeus vannamei) under biofloc condition in land-based ponds

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-02-15 DOI: 10.1016/j.aqrep.2025.102690

Lu Jing , Huan Ren , Wujie Xu , Haochang Su , Xiaojuan Hu , Guoliang Wen , Yu Xu , Lingjie Tu , Yucheng Cao

Land-based pond systems, as an emerging intensive aquaculture model, have encountered developmental constraints due to toxic nitrogen levels. This study aimed to develop and evaluate a biofloc-mediated aquaculture system in land-based ponds, focusing on water quality, bacterial composition of bioflocs, shrimp growth performance, and nonspecific immunity. Juveniles of Litopenaeus vannamei were stocked in three identical land-based barrel tanks at a density of 600 individuals·m⁻² for a 90-day cultivation period, with no water exchange. The results indicated that total ammonia nitrogen (TAN) concentrations were maintained between 0 and 4 mg·L⁻¹, and nitrite (NO₂^--N) levels were kept between 0 and 1.0 mg·L⁻¹. Bacterial community analysis revealed that the richness and Shannon index of bioflocs at 90 days were significantly higher, yet the dominant bacteria throughout the trial remained consistent, comprising Proteobacteria, Actinobacteria, Planctomycetes, Bacteroidetes, and Chloroflexi. Redundancy Analysis (RDA) demonstrated that Bacteroidetes were significantly positively correlated with TAN and NO₂^--N at 30 and 60 days, while Chloroflexi showed a positive correlation with nitrate (NO₃^--N) and phosphate (PO₄^3-) at 90 days. Growth performance metrics indicated a survival rate (SR) of L. vannamei at (78.36 ± 1.81)%, yield of (8.63 ± 0.33) kg·m⁻³, specific growth rate (SGR) of (2.05 ± 0.04)%·d⁻¹, feed conversion rate (FCR) of 1.53 ± 0.25, and water usage of (192.29 ± 9.01) L·kg⁻¹ (shrimp). The relative expression levels of immune-related genes, including SOD, CAT, LZM, proPO, TLR2, and VEGF1L, exhibited an increasing trend over the course of the aquaculture process. Collectively, the findings suggest that bioflocs effectively regulated water quality and enhanced shrimp growth, production, and immune status.

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引用次数: 0

Carbon fluxes and their drivers in integrated shrimp-razor clam culture systems

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-02-15 DOI: 10.1016/j.aqrep.2025.102677

Chunpu Zhao, Shuonan Ma, Zhanfeng Liang, Ying Zeng, Cheng Luo, Jilin Xu

Enhancing carbon sink function in marine aquaculture is among the most promising carbon capture approaches currently available. Ponds for cultivating shrimp and bivalves are the most prevalent aquaculture systems in the coastal areas of China, varying in culture modes with potentially different effects on carbon transformation and transport in these systems. However, quantification of the carbon budget, particularly greenhouse gas carbon emissions from these ponds, is limited, and the underlying driving factors remain unclear. This study aimed to investigate the carbon budget in three shrimp-razor clam culture systems. The findings indicated that feed (shrimp feed and bait-microalgae) and inflow water constituted the primary heterotopic carbon sources in aquaculture systems, comprising 70.4 %–82.4 %. Outflow water was the major component of carbon output, accounting for 35.6 %–53.3 %. Moreover, the tandem culture mode exhibited the most promising CO₂ sink function culture mode, as evidenced by the net CO₂ flux (–10.33 mg m^–2 d^–1) at the water-air interface (WAI). Furthermore, Spearman’s rank correlation and linear mixed models revealed that trophic status was the primary driver of CO₂ fluxes at both the WAI and sediment-water interfaces. Salinity was presumably a contributing factor. Our findings suggest that tandem culture of shrimp and bivalves can be expected to enhance carbon fixation. Optimized culture management practices (feeding management and water exchange) and environmental regulation (e.g., trophic levels and salinity) further contribute to mitigating greenhouse gas emissions.

增强海水养殖的碳汇功能是目前最有前景的碳捕获方法之一。养殖对虾和双壳贝类的池塘是中国沿海地区最普遍的水产养殖系统，不同的养殖模式可能对这些系统中的碳转化和传输产生不同的影响。然而，对这些池塘的碳预算，尤其是温室气体碳排放的量化研究十分有限，其潜在的驱动因素仍不清楚。本研究旨在调查三个对虾-罗盘蛤养殖系统的碳预算。研究结果表明，饲料（虾饲料和饵料-微藻）和流入水构成了水产养殖系统的主要异位碳源，占 70.4%-82.4% 。流出水是碳输出的主要组成部分，占 35.6 %-53.3 %。此外，从水气界面（WAI）的二氧化碳净通量（-10.33 mg m-2 d-1）可以看出，串联养殖模式是最有前途的二氧化碳汇功能养殖模式。此外，斯皮尔曼秩相关和线性混合模型显示，营养状态是 WAI 和沉积物-水界面二氧化碳通量的主要驱动因素。盐度可能也是一个影响因素。我们的研究结果表明，对虾和双壳贝类的串联养殖有望提高碳固定。优化养殖管理方法（饲养管理和水交换）和环境调节（如营养级和盐度）可进一步减少温室气体排放。

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引用次数: 0

Integrating photovoltaic with sea cucumber aquaculture: Environmental impacts and holothurian digestion and aestivation

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-02-15 DOI: 10.1016/j.aqrep.2025.102686

Shuang Guo , Zhenglin Yu , Chaowei Hou , Han Wang , Tongtong Jiang , Lei Pang , Xiao Cong , Qing Wang , Xiutang Yuan

Aquavoltaics, which integrates solar power with aquaculture, offers a promising solution to land-use conflicts between food and energy production while aligning with sustainable development goals. Despite the burgeoning of aquavoltaics, the environmental impacts of photovoltaic (PV) in aquaculture remain poorly understood. We investigated annually the effects of PV on coastal pond environments and cultivated animals. Our results indicated that compared with non-PV pond (nPVP), light intensity in shaded areas of the PV pond (PVP) was significantly reduced by 80.5 ± 12.0 %, thereby water temperature decreased by 1.20 ± 1.25 °C. Plankton species composition showed no significant differences between the two groups. However, Pyrrophyta abundance increased significantly during spring, and zooplankton abundance was significantly reduced in PVP. Sea cucumbers digestive enzyme activities remained unaffected throughout the year. However, aestivation durations were shortened by 12 days in sub-adults and by 1 day in adults in PVP. Piecewise structural equation modeling revealed that light intensity negatively affected temperature, phytoplankton and organic matter (OM), while temperature and phytoplankton negatively affected zooplankton in PVP. Our study demonstrated that PV can effectively reduce water temperatures, enhance sea cucumbers growth by shortening aestivation durations, and have no impact on plankton compositions and sea cucumbers digestive physiology. However, the increased abundance of Pyrrophyta warrant further attention. These findings provide valuable insights into the sustainable development of integrating PV with sea cucumber aquaculture in coastal ponds.

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引用次数: 0

Development and characteristics of artificial interspecific hybridization between Tridacna maxima and T. crocea

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-02-15 DOI: 10.1016/j.aqrep.2025.102694

Jun Li , Haitao Ma , Yanping Qin , Jinkuan Wei , Wanjie Liu , Zhen Zhao , Na Mi , Jiangwei Li , Chao Yue , Qinggan Xing , Ziniu Yu , Yuehuan Zhang

Interspecific hybridization has demonstrated its effectiveness in genetic modification, with the goal of developing novel species that inherits beneficial characteristics from each parent. In the present study, a 2 × 2 factorial hybridization was conducted for the first time, creating four groups: T. maxima♀ × T. maxima♂ (MM), T. crocea♀ × T. crocea♂ (CC), T. maxima♀ × T. crocea♂(MC), and T. crocea♀ × T. maxima♂ (CM). The fertilization and hatching rates of the MC and CM hybrids were similar to those of the intraspecific crosses, achieving satisfactory levels. Compared to CC, MC and CM hybrids also displayed notable growth advantages in shell length and survival rate during juvenile period. Notably, the MC and CM hybrids exhibited substantially faster growth rates compared to the parental giant clams after larval stage, with the highest survival rate (24.47 ± 1.51 %) in these groups at one year old. Moreover, the shell length of the hybrid juveniles significantly exceeded that of the progeny of the two parental species from day 30–360. The shell shape of hybrid offspring was mainly influenced by the maternal parent, whereas the mantle color in MC offspring was influenced by both parents. Like the progenies of pure parents, both hybrids developed normal gonads and were capable of gamete production. All these results indicated that hybrid offspring displayed higher survival rates, faster growth rates, and enhanced mantle colors compared to their parental giant clams. As a result, these hybrids offer great promise for the aquarium trade and aquaculture sectors.

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引用次数: 0

Oral immunization of grouper with recombinant Chlamydomonas to prevent viral nervous necrosis and the effect of which on intestinal microbiota

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-02-15 DOI: 10.1016/j.aqrep.2025.102685

Xiaodong Deng , Dequan Guo , Xiansong Cheng , Peng Hong , Zhijie Li , Sihang Liu , Xiaowen Fei

Although recombinant subunit vaccines are among the safest and most effective currently available, their rapid progress has been hampered by their high cost and need for sophisticated infrastructure. When used as a vehicle for vaccine production, unicellular microalgae are inexpensive, easy to grow and store, and have been shown to fold a variety of vaccine antigens appropriately, allowing for oral delivery of the vaccines possible. The current study investigated the possibility of expressing the grouper nervous necrosis virus (NNV) coat protein in Chlamydomonas reinhardtii as an oral vaccine against viral nervous necrosis(VNN). The identified recombinant algal strains were mixed with commercial feeds at a 1:1 ratio and fed to grouper larva as an immunization feed. The results of the challenge experiments showed that the survival rate of grouper with oral vaccine was increased by 26.7 %-36.7 % compared with the control group, and the amount of brain cavitation was lower in the orally vaccinated grouper. In addition, immune-related genes, including immunoglobulin M(IgM), major histocompatibility complex class I (MHC-Iα), tumour necrosis factor α (TNF-α), interleukin 16 (IL-16), and interleukin 1 beta (IL-1β) were upregulated after oral vaccination. High-throughput 16S rRNA sequencing of grouper gut microbes revealed that immunofeeding altered the structure of the grouper intestinal microbiota, increasing the abundance of antimicrobial and nutrient metabolism-associated microorganisms while decreasing the abundance of pathogenesis-associated harmful microorganisms. The current research shows that recombinant C. reinhardtii producing the NNV coat protein is a promising candidate for oral vaccination against NNV disease in groupers.

{"title":"Oral immunization of grouper with recombinant Chlamydomonas to prevent viral nervous necrosis and the effect of which on intestinal microbiota","authors":"Xiaodong Deng , Dequan Guo , Xiansong Cheng , Peng Hong , Zhijie Li , Sihang Liu , Xiaowen Fei","doi":"10.1016/j.aqrep.2025.102685","DOIUrl":"10.1016/j.aqrep.2025.102685","url":null,"abstract":"<div><div>Although recombinant subunit vaccines are among the safest and most effective currently available, their rapid progress has been hampered by their high cost and need for sophisticated infrastructure. When used as a vehicle for vaccine production, unicellular microalgae are inexpensive, easy to grow and store, and have been shown to fold a variety of vaccine antigens appropriately, allowing for oral delivery of the vaccines possible. The current study investigated the possibility of expressing the grouper nervous necrosis virus (NNV) coat protein in <em>Chlamydomonas reinhardtii</em> as an oral vaccine against viral nervous necrosis(VNN). The identified recombinant algal strains were mixed with commercial feeds at a 1:1 ratio and fed to grouper larva as an immunization feed. The results of the challenge experiments showed that the survival rate of grouper with oral vaccine was increased by 26.7 %-36.7 % compared with the control group, and the amount of brain cavitation was lower in the orally vaccinated grouper. In addition, immune-related genes, including immunoglobulin M(IgM), major histocompatibility complex class I (MHC-Iα), tumour necrosis factor α (TNF-α), interleukin 16 (IL-16), and interleukin 1 beta (IL-1β) were upregulated after oral vaccination. High-throughput 16S rRNA sequencing of grouper gut microbes revealed that immunofeeding altered the structure of the grouper intestinal microbiota, increasing the abundance of antimicrobial and nutrient metabolism-associated microorganisms while decreasing the abundance of pathogenesis-associated harmful microorganisms. The current research shows that recombinant <em>C. reinhardtii</em> producing the NNV coat protein is a promising candidate for oral vaccination against NNV disease in groupers.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102685"},"PeriodicalIF":3.2,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation analysis between carotenoid lyase BCMO1 and the formation of red shell-color in hard clam Meretrix meretrix

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-02-15 DOI: 10.1016/j.aqrep.2025.102674

Heming Shi , Baoyue Cui , Liyuan Lv , Yinghui Dong , Zhihua Lin , Jilin Xu , Hanhan Yao

Carotenoids, the most widespread biological pigments, are responsible for the coloration of animal tissues and surfaces. Yet, little is known about the mechanism of how carotenoids affect the color of clam shells. The hard clam, Meretrix meretrix, exhibits genetic polymorphism leading to diverse shell colors, making it an ideal model for studying carotenoid-based coloring. Cultured strains of M. meretrix with distinct shell colors (red and white) were selected to explore the relationship between carotenoids and shell color. Raman spectroscopy and pigment content analysis revealed that carotenoids primarily contributed to the red shell. The β-carotene-15,15'-monooxygenase 1 (BCMO1) in M. meretrix was cloned, and a phylogenetic analysis was performed. Expression studies showed that Mm-BCMO1 mRNA was consistently expressed during early development, with a notable increase in umbo larvae. High levels of Mm-BCMO1 expression were observed in the hepatopancreas and mantles, particularly in the red strain compared to the white strain. In addition, protein analysis via western blotting (WB) and immunofluorescence (IF) confirmed that Mm-BCMO1 protein levels corresponded to mRNA levels, mainly localized in epithelial cells of the mantle. Moreover, 12 significant single nucleotide polymorphisms (SNPs) were identified in the promoter and coding sequence (CDS) regions of Mm-BCMO1, potentially explaining the color variation between the two shell color strains. These findings indicate that carotenoids, influenced by Mm-BCMO1, play a crucial role in the pigmentation of red shell color. The study provides insights into carotenoid metabolism in M. meretrix and offers valuable information for molecular marker-assisted breeding in clam aquaculture.

{"title":"Correlation analysis between carotenoid lyase BCMO1 and the formation of red shell-color in hard clam Meretrix meretrix","authors":"Heming Shi , Baoyue Cui , Liyuan Lv , Yinghui Dong , Zhihua Lin , Jilin Xu , Hanhan Yao","doi":"10.1016/j.aqrep.2025.102674","DOIUrl":"10.1016/j.aqrep.2025.102674","url":null,"abstract":"<div><div>Carotenoids, the most widespread biological pigments, are responsible for the coloration of animal tissues and surfaces. Yet, little is known about the mechanism of how carotenoids affect the color of clam shells. The hard clam, <em>Meretrix meretrix</em>, exhibits genetic polymorphism leading to diverse shell colors, making it an ideal model for studying carotenoid-based coloring. Cultured strains of <em>M. meretrix</em> with distinct shell colors (red and white) were selected to explore the relationship between carotenoids and shell color. Raman spectroscopy and pigment content analysis revealed that carotenoids primarily contributed to the red shell. The β-carotene-15,15'-monooxygenase 1 (<em>BCMO1</em>) in <em>M. meretrix</em> was cloned, and a phylogenetic analysis was performed. Expression studies showed that <em>Mm-BCMO1</em> mRNA was consistently expressed during early development, with a notable increase in umbo larvae. High levels of <em>Mm-BCMO1</em> expression were observed in the hepatopancreas and mantles, particularly in the red strain compared to the white strain<em>.</em> In addition, protein analysis via western blotting (WB) and immunofluorescence (IF) confirmed that Mm-BCMO1 protein levels corresponded to mRNA levels, mainly localized in epithelial cells of the mantle. Moreover, 12 significant single nucleotide polymorphisms (SNPs) were identified in the promoter and coding sequence (CDS) regions of <em>Mm-BCMO1</em>, potentially explaining the color variation between the two shell color strains. These findings indicate that carotenoids, influenced by Mm-BCMO1, play a crucial role in the pigmentation of red shell color. The study provides insights into carotenoid metabolism in <em>M. meretrix</em> and offers valuable information for molecular marker-assisted breeding in clam aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102674"},"PeriodicalIF":3.2,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precision fish farming: A sensor-based study on Dicentrarchus labrax in a sea cage environment

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-02-14 DOI: 10.1016/j.aqrep.2025.102691

Lola Toomey , Pierluigi Carbonara , Elena Mente , Eva Troianou , Dimitris Troianou , Maria Teresa Spedicato , Giuseppe Lembo , Sébastien Alfonso

The remote real-time monitoring of farmed fish health and welfare without disrupting daily management practices is crucial for the aquaculture industry. This study evaluated the effectiveness of wireless environmental and biological sensors in monitoring fish welfare and gathering insights into potential predictors of mortality and growth of European sea bass (Dicentrarchus labrax). Environmental sensors were deployed within a net pen and eight fish were implanted with pressure and accelerometer sensors to compute acceleration and depth of individual fish. All sensors were connected to a central Hub, enabling real-time visibility for fish farmers, and monitoring was carried out over 68 days. Significant positive correlations were observed between growth and temperature, as well as between growth and dissolved oxygen levels. However, no significant correlations were found between mortality data and any of the parameters considered (environmental, feed load or fish-related traits). Our findings also revealed that fish depth and acceleration, which serves as a proxy for energy expenditure, were sensitive to environmental fluctuations. Fish exhibited a circadian rhythm in both depth and swimming activity, with higher activity in deeper waters during the daytime and lower swimming activity in shallower waters during the night. Temperature, dissolved oxygen, and the amount of feed distributed also influenced fish swimming activity and depth. This study demonstrates that acoustic telemetry, by leveraging biological and environmental data collected via wireless sensors, is a suitable tool to monitor fish directly in sea cages, as well as environmental variations. We also provide initial insights into the potential of predicting the growth performance of European sea bass. Analyses presented here are preliminary and require further refinement, including longer-term observations spanning at least an entire year and the incorporation of additional parameters (e.g. turbidity, water currents). Additionally, a replicability check of the study is necessary, incorporating more cages and other fish farms.

{"title":"Precision fish farming: A sensor-based study on Dicentrarchus labrax in a sea cage environment","authors":"Lola Toomey , Pierluigi Carbonara , Elena Mente , Eva Troianou , Dimitris Troianou , Maria Teresa Spedicato , Giuseppe Lembo , Sébastien Alfonso","doi":"10.1016/j.aqrep.2025.102691","DOIUrl":"10.1016/j.aqrep.2025.102691","url":null,"abstract":"<div><div>The remote real-time monitoring of farmed fish health and welfare without disrupting daily management practices is crucial for the aquaculture industry. This study evaluated the effectiveness of wireless environmental and biological sensors in monitoring fish welfare and gathering insights into potential predictors of mortality and growth of European sea bass (<em>Dicentrarchus labrax</em>). Environmental sensors were deployed within a net pen and eight fish were implanted with pressure and accelerometer sensors to compute acceleration and depth of individual fish. All sensors were connected to a central Hub, enabling real-time visibility for fish farmers, and monitoring was carried out over 68 days. Significant positive correlations were observed between growth and temperature, as well as between growth and dissolved oxygen levels. However, no significant correlations were found between mortality data and any of the parameters considered (environmental, feed load or fish-related traits). Our findings also revealed that fish depth and acceleration, which serves as a proxy for energy expenditure, were sensitive to environmental fluctuations. Fish exhibited a circadian rhythm in both depth and swimming activity, with higher activity in deeper waters during the daytime and lower swimming activity in shallower waters during the night. Temperature, dissolved oxygen, and the amount of feed distributed also influenced fish swimming activity and depth. This study demonstrates that acoustic telemetry, by leveraging biological and environmental data collected via wireless sensors, is a suitable tool to monitor fish directly in sea cages, as well as environmental variations. We also provide initial insights into the potential of predicting the growth performance of European sea bass. Analyses presented here are preliminary and require further refinement, including longer-term observations spanning at least an entire year and the incorporation of additional parameters (e.g. turbidity, water currents). Additionally, a replicability check of the study is necessary, incorporating more cages and other fish farms.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102691"},"PeriodicalIF":3.2,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic comparison between wild-caught and domesticated black tiger shrimp (Penaeus monodon) in early and late-vitellogenic broodstock females

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-02-13 DOI: 10.1016/j.aqrep.2025.102675

Thanh Minh Nguyen , Nhut Minh Tran , Yen Thi Hai Tran , Tham Thi Hong Le , Long Minh Tran , Phuc Nguyen Thien Le , Abigail Elizur , Tomer Ventura , Thu Thi Minh Vo , Tuan Viet Nguyen

Domesticated Penaeus monodon broodstock females (DF) are currently dominating the shrimp industry in Vietnam and globally. However, the downgraded quality of DF compromises the sustainability of P. monodon production. Compared with DF, wild-caught broodstock females (WF) exhibit superior survival rate, offspring quality, and anti-pathogen capability, attracting interest from the seed production sector. To understand their reproductive biology and maturation at a molecular level, we conducted a comprehensive transcriptomic analysis of P. monodon broodstock females from domestication and wild sources. We performed a comparative analysis for ovaries, hepatopancreas, and eyestalks at previtellogenesis (stage I) and late cortical rod stage (stage IV). The analysis generated 13,801 differentially expressed genes (DEGs), of which the number of downregulated genes exceeded that of upregulated genes at stage I. Similarly, the number of downregulated genes surpassed the number of upregulated genes in stage IV, apart from hepatopancreas, where the number of upregulated genes increased compared to the downregulated ones. This expression pattern suggests that the number of DEGs depends on the vitellogenic stages and tissues where they are expressed. A comparative DEG profiling study of WF and DF highlights the key genes that are likely to enhance the reproductive traits, classified into signaling and transporting in stage I, anti-pathogens, molting and reproduction genes in stage IV, deepening our understanding of the molecular events during vitellogenic stages. Our study provides candidate genes for genetic selection and/or gene manipulation to improve the reproductive traits in the domesticated P. monodon broodstock females.

{"title":"Transcriptomic comparison between wild-caught and domesticated black tiger shrimp (Penaeus monodon) in early and late-vitellogenic broodstock females","authors":"Thanh Minh Nguyen , Nhut Minh Tran , Yen Thi Hai Tran , Tham Thi Hong Le , Long Minh Tran , Phuc Nguyen Thien Le , Abigail Elizur , Tomer Ventura , Thu Thi Minh Vo , Tuan Viet Nguyen","doi":"10.1016/j.aqrep.2025.102675","DOIUrl":"10.1016/j.aqrep.2025.102675","url":null,"abstract":"<div><div>Domesticated <em>Penaeus monodon</em> broodstock females (DF) are currently dominating the shrimp industry in Vietnam and globally. However, the downgraded quality of DF compromises the sustainability of <em>P. monodon</em> production. Compared with DF, wild-caught broodstock females (WF) exhibit superior survival rate, offspring quality, and anti-pathogen capability, attracting interest from the seed production sector. To understand their reproductive biology and maturation at a molecular level, we conducted a comprehensive transcriptomic analysis of <em>P. monodon</em> broodstock females from domestication and wild sources. We performed a comparative analysis for ovaries, hepatopancreas, and eyestalks at previtellogenesis (stage I) and late cortical rod stage (stage IV). The analysis generated 13,801 differentially expressed genes (DEGs), of which the number of downregulated genes exceeded that of upregulated genes at stage I. Similarly, the number of downregulated genes surpassed the number of upregulated genes in stage IV, apart from hepatopancreas, where the number of upregulated genes increased compared to the downregulated ones. This expression pattern suggests that the number of DEGs depends on the vitellogenic stages and tissues where they are expressed. A comparative DEG profiling study of WF and DF highlights the key genes that are likely to enhance the reproductive traits, classified into signaling and transporting in stage I, anti-pathogens, molting and reproduction genes in stage IV, deepening our understanding of the molecular events during vitellogenic stages. Our study provides candidate genes for genetic selection and/or gene manipulation to improve the reproductive traits in the domesticated <em>P. monodon</em> broodstock females.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102675"},"PeriodicalIF":3.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acetylation inhibition of key lysine residue enhances antibacterial activity of rhamnose-binding lectin in pearl oyster, Pinctada fucata martensii

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports

Pub Date : 2025-02-13 DOI: 10.1016/j.aqrep.2025.102683

Jie Chen , Zhijie Guo , Haiying Liang , Qiyuan Zhang , Yifan Wu

Pearl oyster, Pinctada fucata martensii (Pm) is a marine bivalve that plays a key role in pearl cultivation in the South China Sea. Rhamnose-binding lectin (RBL) is an essential component of its immune system. Recently, the regulatory role of acetylation in the innate immune response has gained significant attention. In this study, we investigated the impact of acetylation on PmRBL by constructing recombinant (r) PmRBL proteins with acetylation and deacetylation modifications at lysine residues K91 and K159 (rPmRBL: rPmRBL-K91R, rPmRBL-K91Q, rPmRBL-K159Q, and rPmRBL-K159R), and performing antibacterial and agglutination assays. The antibacterial assays showed that rPmRBL-K159R exhibited enhanced inhibition against specific bacteria. Additionally, the agglutination assays revealed that rPmRBL-K159R displayed stronger aggregation effects on Aeromonas hydrophila and Escherichia coli compared to rPmRBL-Wild. These findings suggest that acetylation at K91 and K159 influences the immune function of PmRBL, with decreased acetylation at K159 potentially enhancing its antibacterial activity and altering its bacterial targets, making K159 a key regulatory site. This study provides new insights into the immune function of PmRBL from the perspective of acetylation modification, offering a deeper understanding of the regulatory mechanisms involving K91 and K159, and presenting new directions for exploring the immune regulation of P. f. martensii.

{"title":"Acetylation inhibition of key lysine residue enhances antibacterial activity of rhamnose-binding lectin in pearl oyster, Pinctada fucata martensii","authors":"Jie Chen , Zhijie Guo , Haiying Liang , Qiyuan Zhang , Yifan Wu","doi":"10.1016/j.aqrep.2025.102683","DOIUrl":"10.1016/j.aqrep.2025.102683","url":null,"abstract":"<div><div>Pearl oyster, <em>Pinctada fucata martensii</em> (Pm) is a marine bivalve that plays a key role in pearl cultivation in the South China Sea. Rhamnose-binding lectin (RBL) is an essential component of its immune system. Recently, the regulatory role of acetylation in the innate immune response has gained significant attention. In this study, we investigated the impact of acetylation on PmRBL by constructing recombinant (r) PmRBL proteins with acetylation and deacetylation modifications at lysine residues K91 and K159 (rPmRBL: rPmRBL-K91R, rPmRBL-K91Q, rPmRBL-K159Q, and rPmRBL-K159R), and performing antibacterial and agglutination assays. The antibacterial assays showed that rPmRBL-K159R exhibited enhanced inhibition against specific bacteria. Additionally, the agglutination assays revealed that rPmRBL-K159R displayed stronger aggregation effects on <em>Aeromonas hydrophila</em> and <em>Escherichia coli</em> compared to rPmRBL-Wild. These findings suggest that acetylation at K91 and K159 influences the immune function of PmRBL, with decreased acetylation at K159 potentially enhancing its antibacterial activity and altering its bacterial targets, making K159 a key regulatory site. This study provides new insights into the immune function of PmRBL from the perspective of acetylation modification, offering a deeper understanding of the regulatory mechanisms involving K91 and K159, and presenting new directions for exploring the immune regulation of <em>P. f. martensii</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"41 ","pages":"Article 102683"},"PeriodicalIF":3.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0