Aquaculture Reports最新文献

{"title":"Cadmium accumulation characteristics and dietary risks in three aquatic animals with different habitat characteristics in a rice-fish co-culture system","authors":"Wei Luo ,&nbsp;Yibo Zhang ,&nbsp;Shoudong Zhang ,&nbsp;Kunpu Sun ,&nbsp;Ke Li ,&nbsp;Feifei He ,&nbsp;Jixian Huang ,&nbsp;Shiyong Yang ,&nbsp;Zongjun Du","doi":"10.1016/j.aqrep.2024.102417","DOIUrl":"10.1016/j.aqrep.2024.102417","url":null,"abstract":"<div><div>The safety of the rice-fish co-culture system (RFCS) is threatened by severe soil cadmium (Cd) pollution. However, the characteristics of Cd accumulation in aquatic animals within this system remain poorly understood. To investigate the Cd accumulation characteristics and dietary risks in aquatic animals with varying habitat characteristics, three species — crayfish <em>Procambarus clarkii</em>, loach <em>Paramisgumus dabryanus</em>, and crucian carp <em>Carassius auratus</em> — were cultured in RFCS with soil Cd pollution ranging from 0.2 to 32.0 mg/kg. The results demonstrated that the survival of the three aquatic animals did not differ significantly at medium to low Cd concentrations (0.2–4 mg/kg) but decreased markedly at high concentrations (16–32 mg/kg). The WGR of crayfish decreased as the Cd treatment concentration increased. The Cd accumulation in various tissues showed an increasing trend with increasing Cd exposure. At the same concentration, the accumulation of Cd was in the order of liver (or hepatopancreas) ≈ intestine &gt; gill &gt; muscle. At the same Cd treatment concentration, the Cd accumulation in different animal species generally showed a trend of crayfish &gt; loach &gt; crucian carp, indicating that accumulation of Cd in the aquatic animals within the Cd-contaminated RFCS is closely related to their habitat characteristics. The soil pollution thresholds for crayfish, loach and crucian carp were determined to be 11.7, 38.8 and 90.5 mg/kg, respectively, which can serve as early warnings for safe production. Within the tested concentration range, the aquatic animals did not pose non-carcinogenic risks; however, they posed carcinogenic risks when these animals are exposed to high soil Cd concentrations.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102417"},"PeriodicalIF":3.2,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Balancing act: How cholesterol and phospholipids influence juvenile mud crab Scylla paramamosain growth and lipid metabolism","authors":"Teng Liu ,&nbsp;Hanying Xu ,&nbsp;Wenping Feng ,&nbsp;Jiale He ,&nbsp;Tao Han ,&nbsp;Jiteng Wang ,&nbsp;Qingyang Wu ,&nbsp;Chunlin Wang","doi":"10.1016/j.aqrep.2024.102453","DOIUrl":"10.1016/j.aqrep.2024.102453","url":null,"abstract":"<div><div>A 2 × 3 two-factor experiment was designed to assess the effects of dietary cholesterol (CHO) and phospholipids (PLs) on growth and lipid metabolism in early juvenile mud crabs (0.01 g crab⁻¹). The experimental diets were designed with two CHO levels: 0.40 % (LCH) and 0.80 % (HCH), and three PLs levels: 1.80 % (LPL), 2.50 % (MPL), and 3.20 % (HPL). The 58-day aquaculture trial demonstrated that the LCH-HPL group achieved the best growth performance in mud crabs, characterized by the highest final body weight, weight gain, and specific growth rate. Regarding whole-body composition, dietary PLs increased total cholesterol (T-CHO), PLs, triglyceride (TG), and crude lipid content in the LCH groups. Mud crabs in the HCH groups had a higher proportion of saturated fatty acids, a lower proportion of monounsaturated fatty acids, and increased gene expression of sterol regulatory element binding protein 1c (<em>srebp-1c</em>) and fatty acid synthase (<em>fas</em>) compared to those in the LCH groups. As the dietary PLs increased, mud crabs in the LCH groups exhibited up-regulation in the expression of genes, including <em>srebp-1c</em>, <em>fas</em>, elongation of very long-chain fatty acid protein 4 (<em>elovl4</em>), <em>elovl6</em>, and Δ9-fatty acid desaturase (<em>Δ9-fad</em>). In the HCH groups, elevated dietary PLs resulted in the down-regulation of fatty acid binding protein (<em>fabp</em>) gene expression. In addition, high levels of dietary CHO and PLs (HCH-HPL) inhibited the catalase activity of mud crabs, resulting in a significant increase in the malondialdehyde content. Correlation analysis revealed that dietary CHO was positively correlated with the expression of long-chain polyunsaturated fatty acids synthesizing genes, and dietary PLs were positively correlated with the whole-body lipid content of mud crabs. Additionally, a positive correlation was found between the growth performance and whole-body content of crude protein, crude lipid, and antioxidant capacity in mud crabs.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102453"},"PeriodicalIF":3.2,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant leptins affect lipid metabolism in hepatocytes of Cynoglossus semilaevis","authors":"Xin Cai ,&nbsp;Yuting Li ,&nbsp;Zhaojun Meng ,&nbsp;Aijun Cui ,&nbsp;Yan Jiang ,&nbsp;Yongjiang Xu","doi":"10.1016/j.aqrep.2024.102423","DOIUrl":"10.1016/j.aqrep.2024.102423","url":null,"abstract":"<div><div>Leptin is a peptide hormone primarily produced by adipose tissue, which plays a crucial role in regulating energy balance by controlling appetite and energy expenditure. To better understand the intricate physiological functions of leptin in hepatocytes in tongue sole (<em>Cynoglossus semilaevis</em>), this study presents an integrated transcriptomic and metabolomic analysis of tongue sole hepatocytes following stimulation with lepA and lepB. Comparative analysis identified 2971 and 2900 upregulated differentially expressed genes (DEGs) and 1895 and 1821 downregulated DEGs in response to lepA and lepB stimulation, respectively. Notably, 5706 genes were commonly regulated by both stimulations, suggesting overlapping functional roles of these two leptin proteins. GO and KEGG enrichment analysis indicated significant enrichment in immune response (JAK-STAT signaling pathway, NF-κB signaling pathway, etc.) and lipid metabolism pathways, such as fatty acid synthesis, with similar findings for lepB. Metabolomic analysis demonstrated clear separation between treatment and control groups, with 34 medium- to long-chain fatty acids and numerous differentially expressed metabolites (DEMs) identified. KEGG analysis of the metabolome paralleled the transcriptomic findings, with shared pathways in lipid metabolism and immune function. Finally, joint analysis highlighted co-enrichment in linoleic acid metabolism and leishmaniasis pathways. Detailed mechanistic insights revealed the activation of JAK-STAT and NF-κB signaling pathways, modulation of arachidonic acid metabolism, and the influence on the MAPK signaling pathway. Additionally, lepA uniquely co-enriched in the fatty acid synthesis pathway, with specific gene regulation affecting the synthesis of various fatty acids. The study concluded that lepA and lepB exerted significant regulatory effects on lipid metabolism and immune responses in tongue sole hepatocytes through complex molecular networks. To our current knowledge, this represents the first direct evidence of leptin's role in immune function within teleost fish and offers valuable insights into the molecular mechanisms of lipid metabolism underlying the actions of lepA and lepB.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102423"},"PeriodicalIF":3.2,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bovine bone meal combined with dephenolized cottonseed protein is an effective fishmeal substitution in Trachinotus ovatus feed: A comprehensive evaluation of growth, muscle quality, and intestinal digestion physiology","authors":"Zeliang Su ,&nbsp;Fang Chen ,&nbsp;Junfeng Guan ,&nbsp;Yongcai Ma ,&nbsp;Chao Xu ,&nbsp;Yuanyou Li ,&nbsp;Dizhi Xie","doi":"10.1016/j.aqrep.2024.102444","DOIUrl":"10.1016/j.aqrep.2024.102444","url":null,"abstract":"<div><div>To investigate the feasibility of substituting fishmeal (FM) with a combination of bovine bone meal (BBM) and dephenolized cottonseed protein (DCSP), a mixture (consisting of 60 % BBM and 40 % DCSP) was used to replace varying FM levels in Golden pompano (<em>Trachinotus ovatus</em>) feed. Five isoproteic (47 %) and isolipidic (12 %) experimental diets were formulated, with FM levels set at 30 %, 24 %, 18 %, 12 %, and 6 % (namely Control, FM24, FM18, FM12, and FM6). To compensate for the reduced FM content, BBM and DCSP were used as substitutes, and DL-methionine and L-lysine were added to maintain the dietary amino acid balance. Following a 63-day trial, results showed that the growth of FM24, FM18 and FM12 groups did not exhibit significant differences compared to the Control group, while those of FM6 group considerably decreased. The biochemical composition of whole fish did not display significant differences among the five groups, while the muscle protein contents of the FM12 group were higher than the Control group. Most of the muscle texture properties were positively modified in the FM 12 and FM 6 groups. With the substitution ratio increase, the contents of serum total protein, albumin, total amino acids, and cholecystokinin were elevated, while serum peptide YY contents decreased. In addition, intestinal trypsin, NaK-ATPase, and alkaline phosphatase activities in FM18, FM12, and FM6 groups decreased, and lipase activities exhibited the opposite trend. The transcription levels of genes related to amino acid-sensing receptors (<em>asct2</em> and <em>snat2</em>) were down-regulated with increasing dietary FM substitutions, while the expression levels of appetite regulation factors (<em>pept1, cck,</em> and <em>ghrl)</em> were up-regulated. In conclusion, the FM content in the diet of <em>T. ovatus</em> can be reduced to 12 % through the utilization of a combination of BBM and DCSP, with no adverse effects observed on growth performance, biochemical composition, or muscle texture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102444"},"PeriodicalIF":3.2,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of temperature on the digestibility of non-starch polysaccharide-rich ingredients in Nile tilapia (Oreochromis niloticus)","authors":"B.K. Dey ,&nbsp;M.C.J. Verdegem ,&nbsp;M.A.J. Nederlof ,&nbsp;J.M. Beddow ,&nbsp;K. Masagounder ,&nbsp;J. Mas-Muñoz ,&nbsp;J.W. Schrama","doi":"10.1016/j.aqrep.2024.102426","DOIUrl":"10.1016/j.aqrep.2024.102426","url":null,"abstract":"<div><div>This study aimed to investigate the interaction effect of NSP-rich ingredient and rearing temperature on nutrient digestibility of the ingredients in Nile tilapia. Seven NSP-rich ingredients were tested: insect meal, wheat bran, palm kernel meal, rapeseed meal, sunflower meal, dried distillers grains with solubles (DDGS) from corn, and rice bran. A balanced control diet was formulated followed by the formulation of seven test diets by mixing 30 % of each test ingredient with 70 % of the control diet and fed to fish reared at 24 or 32 °C. At each temperature, 35 all male Nile tilapia with an initial mean weight of 45.0 g at 24 °C and 43.8 g at 32 °C were housed in each of 24 glass-tanks, each containing 60 L of water. The diets were administered to the fish groups in triplicate over 42 d in a recirculating aquaculture system. Fecal samples were collected and analyzed alongside the diets to determine nutrient digestibility. Digestibility of all nutrients differed between ingredients (<em>P</em> &lt; 0.001) at both temperatures. Wheat bran had the lowest NSP digestibility at both 24 (1.9 %) and 32 °C (9.7 %). At 24 °C, DDGS showed the highest NSP and protein digestibility (41.4 and 90.3 %), while at 32 °C insect meal showed the highest NSP and fat digestibility (59.8 and 97.5 %). Insect meal showed the highest fat digestibility (97.4 %) also at 24 °C. An interaction effect between ingredient and temperature was observed for the digestibility of NSP, carbohydrate, protein, amino acids, magnesium, and dry matter (<em>P</em> ≤ 0.035). Some nutrients showed reduced digestibility for some ingredients at the higher temperature, but in most of the cases temperature increased nutrient digestibility, with insect meal exhibiting a 20 % increase in NSP digestibility. In conclusion, water temperature affect nutrient digestibility, but the extent of the temperature effect differs between ingredients.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102426"},"PeriodicalIF":3.2,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary fish meal replacement by Periplaneta americana meal on growth, metabolism, intestinal health and resistance against Aeromonas hydrophila of Micropterus salmoides","authors":"Yuanyi Liu,&nbsp;Changchang Pu,&nbsp;Zihui Wei,&nbsp;Yang Wang,&nbsp;Weichuan Zhang,&nbsp;Hongyu Chen,&nbsp;Yong Huang","doi":"10.1016/j.aqrep.2024.102445","DOIUrl":"10.1016/j.aqrep.2024.102445","url":null,"abstract":"<div><div>An 8-week culture experiment was conducted to investigate the effects of partial replacement of fish meal (FM) with <em>Periplaneta americana</em> meal (PAM) on growth performance, body composition, serum biochemical parameters, intestinal health and resistance against <em>Aeromonas hydrophila</em> of largemouth bass, <em>Micropterus salmoides</em>. A total of 450 healthy largemouth bass with an initial body weight (IBW) of 14.55±0.09 g were randomly divided into five groups. Five experimental diets were prepared by replacing 0 % (P0), 15 % (P15), 30 % (P30), 45 % (P45) and 60 % (P60) of the FM in the basal diet with PAM, respectively. The results showed that final body weight (FBW), weight gain rate (WGR), and specific growth rate (SGR) were no significant difference in P15 and P30 compared with the control group (<em>P</em> &gt; 0.05). Crude protein of whole fish body was significantly increased and crude lipid was significantly decreased in all PAM groups (<em>P</em> &lt; 0.05). In the P30, the antioxidant enzyme activities were significantly higher than those in the control and other experimental groups, and malondialdehyde (MDA) was significantly decreased (<em>P</em> &lt; 0.05). The activities of α-amylase and pepsin increased significantly in P15 and P30 and decreased significantly in P45 and P60 (<em>P</em> &lt; 0.05). Lipase was significantly increased in all groups except P15 than in the control group (<em>P</em> &lt; 0.05). There were no significant differences in villus height (VH), crypt depth (CD), and muscle thickness (MT) among all groups (<em>P</em> &gt; 0.05), but villus width (VW) was significantly decreased in P45 and P60 (<em>P</em> &lt; 0.05). The relative expression of <em>IL-10</em> and <em>TGF-β1</em> were first up-regulated and then down-regulated, while <em>IL-8</em> was opposite (<em>P</em> &lt; 0.05). The relative expression of <em>Casp3</em>, <em>Casp9</em> and <em>IFN-γ</em> were significantly up-regulated in P45 and P60 (<em>P</em> &lt; 0.05). After injecting <em>A. hydrophila</em>, all groups of largemouth bass experienced mortality, but cumulative mortality was lowest in the P30. In conclusion, PAM can partially replace FM in the diet of juvenile largemouth bass. The replacement of no more than 30 % is unlikely to adversely affect growth performance, and can increase the crude protein content of the whole fish body, and is beneficial to intestinal health.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102445"},"PeriodicalIF":3.2,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of freshness of poultry by-product meal on the growth performance, immune response, and hepatopancreatic health of Macrobrachium rosenbergii","authors":"Jie Xu ,&nbsp;Kangyu Deng ,&nbsp;Enhui Chang ,&nbsp;Xin Zhang ,&nbsp;Yu Fu ,&nbsp;Haoyue Guo ,&nbsp;Yuheng Wu ,&nbsp;Anran Wang ,&nbsp;Deng Deng ,&nbsp;Shuyan Miao","doi":"10.1016/j.aqrep.2024.102443","DOIUrl":"10.1016/j.aqrep.2024.102443","url":null,"abstract":"<div><div>A 73-day feeding trial was conducted to determine the effects of the freshness of poultry by-product meal on the growth performance, immune response and hepatopancreatic health of giant freshwater prawn, <em>Macrobrachium rosenbergii.</em> Three semi-purified diets were formulated containing 2.5 % poultry by-product meal with different TVB-N value, designated as L group (untreated poultry by-product meal, TVB-N 10 mg/100 g, i.e., dietary TVB-N 55 mg/100 g), M group (TVB-N 300 mg/100 g, i.e., dietary TVB-N 60 mg/100 g), and H group (TVB-N 450 mg/100 g, i.e., dietary TVB-N 68 mg/100 g), and fed to three groups of prawns (initial weight: 0.82 ± 0.01 g), respectively. Results showed that the weight gain rate (WGR) and specific growth rate (SGR) of prawns significantly decreased with the TVB-N value increased (<em>P</em> &lt; 0.05). The survival rate (SR) and hepatosomatic index (HSI) of prawns in group M and group H were significantly decreased (<em>P</em> &lt; 0.05), while the feed conversion ratio (FCR) was significantly increased (<em>P</em> &lt; 0.05). In addition, the lipase (LPS) and α-amylase (α-AMS) activity of prawns in group L was significantly higher than that in group H (<em>P</em> &lt; 0.05). With the poultry by-product meal TVB-N value increased, the activity of total superoxide dismutase (T-SOD) and glutathione S-transferase (GST) was significantly decreased. RT-qPCR results showed that the expression of <em>bok</em> in group H was significantly higher than that in group L and group M (<em>P</em> &lt; 0.05), the expressions of <em>caspase3</em>, <em>caspase9</em>, <em>bax</em>, and <em>nf-κb</em> in group H were significantly higher than that in group L (<em>P</em> &lt; 0.05). High temperature stress and ammonia nitrogen stress significantly decreased the cumulative survival rate of prawns in group M and group H. Conclusively, it is necessary to guarantee the freshness of the ingredients in prawn formulated diets, and the TVB-N value of diets is recommended not to exceed 60 mg/100 g.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102443"},"PeriodicalIF":3.2,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enriched calanoid copepods Acartia tonsa (Dana, 1849) enhances growth, survival, biochemical composition and morphological development during larval first feeding of the orchid dottyback Pseudochromis fridmani (Klausewitz, 1968)","authors":"Andrea Martino ,&nbsp;Daniel Montero ,&nbsp;Javier Roo ,&nbsp;Pedro Castro ,&nbsp;Silvia Lavorano ,&nbsp;Francisco Otero-Ferrer","doi":"10.1016/j.aqrep.2024.102437","DOIUrl":"10.1016/j.aqrep.2024.102437","url":null,"abstract":"<div><div>In a time of unprecedented coral reef decline, improved aquaculture protocols and high-quality live feeds may contribute to the sustainability of the ornamentals industry producing new valuable and stronger specimens bred under controlled conditions, mitigating the pressure on wild natural stocks. In the present study, the effect of microalgae enriched live feed diets (15 ind. mL⁻¹), the copepod <em>Acartia tonsa</em> and the rotifer <em>Brachionus plicatilis,</em> were tested on early larvae stages (0–15 days post hatch) of the orchid dottyback <em>Pseudochromis fridmani</em>. Enriched <em>A. tonsa</em> significantly improved <em>P. fridmani</em> larval growth, survival, biochemical composition, as well as the morphological development of liver, intestine and skeletal system, compared to rotifers treatment. The results obtained showed that is feasible to totally replace the enriched rotifer <em>B. plicatilis</em> with the enriched copepod <em>A. tonsa</em> during first larval feeding of orchid dottybacks, resulting in a more robust larvae with better culture performance. Indeed, feeding larvae with enriched <em>A. tonsa</em> avoid the different negative effects when used enriched rotifers diets, which led to liver steatosis and nuclear pyknosis, delays in intestine and skeletal development, altered proximate composition and FA content. This study provided valuable information about rearing and breeding under controlled conditions of <em>P. fridmani</em>, contributing to its mass scale production and to the implementation of new hatchery protocols employing high quality live feeds.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102437"},"PeriodicalIF":3.2,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different feeding patterns on growth, enzyme activity, and intestinal microbiome of the juvenile Pacific abalone Haliotis discus hannai","authors":"Zhansheng Guo ,&nbsp;Xuguang Hou ,&nbsp;Lirong Chang ,&nbsp;Zongjun Du ,&nbsp;Kuntao Shi ,&nbsp;Aihuan Song ,&nbsp;Zhenlin Liang ,&nbsp;Jiequan Gu","doi":"10.1016/j.aqrep.2024.102427","DOIUrl":"10.1016/j.aqrep.2024.102427","url":null,"abstract":"<div><div>In northern China, the predominant food sources for abalone farming include fresh kelp, salted kelp, and formulated diets, and abalone can also endure prolonged starvation when food is scarce. While there is a dearth of comprehensive investigation into the effects of these feeding patterns on the temporal dynamics of enzyme activity, and intestinal microbiome of abalone. In this study, a total of 3600 juvenile Pacific abalones were allocated to 12 tanks, randomly divided into four groups based on the feeding patterns: fresh kelp (labeled as FK), salted kelp (SK), formulated feed (FF), and starved group (S). After a 60-day feeding trial, the results showed that abalone in the FK, SK, and FF groups exhibited significant positive effects on both weight and shell growth compared to the S group (<em>P</em>&lt;0.05). Notably, the FF group displayed the highest values of the growth performance. The activity of digestive enzymes and antioxidant enzymes in the FF group had an up-regulated trend over the course of feeding trial, whereas amylase and lipase activity in abalone declined along with starvation. Moreover, the microbial Shannon index showed an overall decreasing trend over time in the FF, FK, and SK groups except for the S group. The ANOSIM analysis revealed that both feeding pattern and cultivating time significantly influenced the structure of the microbial communities. Across the feeding trial, Proteobacteria, Fusobacteriota, and Firmicutes were identified as the dominant phyla in the intestinal microbiome of abalone under different feeding patterns. The core genera were <em>Psychrilyobacter</em>, <em>Mycoplasma</em>, and <em>Vibrio</em>. Additionally, the neutral model analysis revealed that stochastic processes both play a key role in shaping the assembly of microbial communities in different feeding patterns. This study could offer valuable insights into the impact of different feeding patterns on growth and physiology of abalone, thereby contributing to the enhancement of management strategies in the northern Chinese abalone aquaculture industry.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102427"},"PeriodicalIF":3.2,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An emerging role of ferulic acid on sub-adult grass carp (Ctenopharyngodon idellus): Increasing protein digestion and regulating amino acid and oligopeptide transporters expression","authors":"Xu-yuan Shao ,&nbsp;Pei Wu ,&nbsp;Wei-dan Jiang ,&nbsp;Yang Liu ,&nbsp;He-qun Shi ,&nbsp;Rui-nan Zhang ,&nbsp;Lin Feng ,&nbsp;Xiao-qiu Zhou","doi":"10.1016/j.aqrep.2024.102438","DOIUrl":"10.1016/j.aqrep.2024.102438","url":null,"abstract":"<div><div>This study was conducted to evaluate the effect of supplement ferulic acid on growth performance, digestive ability and transport function of amino acids and oligopeptides in the intestine of sub-adult grass carp (<em>Ctenopharyngodon idellus</em>). The experiment was divided into two parts: a. Digestion experiment: healthy grass carp (678.83±1.00 g) were divided into two groups: control group (0 mg/kg FA) and ferulic acid group (100 mg/kg FA) for 2 weeks; b. Growth experiment: healthy grass carp (678.83±1.00 g) were divided into five treatment groups, the control group (FA0) with no ferulic acid and the experimental group supplemented with ferulic acid 50 (FA50), 100 (FA100), 150 (FA150) and 200 (FA200) mg/kg feed for 9 weeks. The results showed that a. Ferulic acid supplementation increased the apparent digestibility of crude protein and crude fat in grass carp. b. Ferulic acid supplementation improved the growth performance and feed efficiency (FE) of grass carp; increased the activities of trypsin and Na⁺/K⁺-ATPase; and up-regulated the levels of mRNAs of some neutral and cationic (non-anionic) amino acid transporter proteins (Solute Carrier Family7member1 (SLC7A7), SLC7A8, etc.) and H⁺/oligopeptide transporter protein 1 (PepT1) gene and protein levels. In addition, ferulic acid promoted the gene expression levels of protein kinase B (Akt), target of rapamycin (TOR); up-regulated the mRNA levels of tail-side-associated homologous frame transcription factor 2 (Cdx2), cAMP response element-binding protein (CREB), and transcriptional co-activator-specific protein 1 (Sp1), and down-regulated the gene and protein levels of protein kinase CβII (PKCβII). In a word, dietary ferulic acid promoted growth performance, digestion, as well as amino acid and oligopeptide transport capacities. Finally, based on regression analyses of percentage weight gain (PWG), FE, specific growth rate (SGR), intestine fold height, trypsin activity, and Na⁺/K⁺-ATPase activity, we recommend optimal supplementation levels of ferulic acid in sub-adult grass carp of 92.18–120.60 mg/kg diet.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102438"},"PeriodicalIF":3.2,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}