Aquaculture Reports最新文献

{"title":"Effects of Probiotics Red Yeast Rhodotorula paludigena CM33 on enhancing color pigmentation, antioxidant activity, immune response, intestinal microbiota, and growth in a commercial ornamental fish: Flowerhorn fish","authors":"Jirawadee Kaewda ,&nbsp;Papungkorn Sangsawad ,&nbsp;Surintorn Boonanuntanasarn ,&nbsp;Pimpisut Manassila ,&nbsp;Apichat Boontawan ,&nbsp;Mariena Ketudat‑Cairns ,&nbsp;Cheeranan Sriphuttha ,&nbsp;Chatsirin Nakharuthai","doi":"10.1016/j.aqrep.2024.102609","DOIUrl":"10.1016/j.aqrep.2024.102609","url":null,"abstract":"<div><div>The application of novel feed additives, including yeast-based probiotics, has emerged as a strategy to enhance growth, health, and aesthetic appeal in ornamental fish. This study evaluated the probiotic properties of red yeast <em>Rhodotorula paludigena</em> CM33 in flowerhorn fish (initial weight 9.81 ± 1.25 g). Forty fish were randomly distributed into individual glass tanks and subjected to four feeding treatments: a non-supplemented control diet and diets supplemented with 0.5 %, 1 %, and 2 % <em>R. paludigena</em> CM33 for 105 days. Results indicated that <em>R. paludigena</em> CM33 exhibits comprehensive characteristics validating it as a potential probiotic, demonstrating resistance to various bile salts, pH levels, and thermotolerance. It presented significant antioxidant activity, auto-aggregation, hydrophobicity, positive catalase activity, non-hemolytic properties, and resistance to 14 tested antibiotics. After the feeding trial, fish fed diets supplemented with 1 % and 2 % <em>R. paludigena</em> CM33 showed significant improvements in growth performance, lysozyme activity, total immunoglobulin levels, and antioxidant enzyme activity. These findings correspond with the mRNA expression results of growth-related genes (insulin-like growth factor-1 (<em>IGF-I</em>) and <em>IGF-II</em>), antioxidant genes (superoxide dismutase (<em>SOD</em>) and catalase (<em>CAT</em>)), and pro-inflammatory genes (interleukin-1 beta (<em>IL-1β</em>) and <em>IL-6</em>). Microbiome analysis indicated that incorporating 1 % <em>R. paludigena</em> CM33 into the diet reduced species richness in the intestinal tract of flowerhorn fish. However, both 1 % and 2 % <em>R. paludigena</em> CM33 increased the abundance of <em>Cetobacterium</em>. Field emission scanning electron microscopy confirmed the colonization of <em>R. paludigena</em> CM33 in the intestines of treated fish; meanwhile, fish skin coloration and carotenoid accumulation demonstrated the color enhancement properties of <em>R. paludigena</em> CM33 in flowerhorn fish. These findings suggest that <em>R. paludigena</em> CM33 could be a promising sustainable aquafeed ingredient for the ornamental aquaculture industry.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102609"},"PeriodicalIF":3.2,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a dynamic energy budget model to the blood clam, Tegillarca granosa, reared in culture pond","authors":"Lin He ,&nbsp;Bo Jiang ,&nbsp;Xiafei Zheng ,&nbsp;Julin Yuan ,&nbsp;Zhihua Lin","doi":"10.1016/j.aqrep.2024.102602","DOIUrl":"10.1016/j.aqrep.2024.102602","url":null,"abstract":"<div><div>Dynamic Energy Budget (DEB) theory seeks to quantify the energetic framework of individual organisms through a dynamic model that encompasses food uptake and its subsequent utilization in metabolic processes. This paper aims to extend the existing DEB model for <em>Tegillarca granosa</em> in pond culture. The model is influenced by water temperature and food density, which serve as key forcing variables throughout larval development. We calculated DEB parameter values for larvae through laboratory experiments specifically designed to gather datasets on ingestion and growth under varying phytoplankton densities and temperatures. Sensitivity analysis indicated that Arrhenius temperature (<em>T</em>_<em>A</em>) exerted the most significant impact on growth, with a 10 % increase in <em>T</em>_<em>A</em> resulting in an 11.6 % increase in dry tissue weight. Insufficient energy allocated to storage during January and February hindered the growth of <em>T. granosa</em>, leading to reduced dry tissue weight, structural damage, and decreased survival rates during winter. This study provides theoretical insights into assessing the carrying capacity and implementing scientifically informed aquaculture management practices for <em>T. granosa</em> in pond systems.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102602"},"PeriodicalIF":3.2,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Macleaya cordata extract supplementation in low-protein, low-fishmeal diets on growth, hematology, intestinal histology, and microbiota in largemouth bass (Micropterus salmoides)","authors":"Wenyuan Xu ,&nbsp;Ruijun Zhu ,&nbsp;Dianyu Huan ,&nbsp;Xiaoqin Li ,&nbsp;Xiangjun Leng","doi":"10.1016/j.aqrep.2025.102618","DOIUrl":"10.1016/j.aqrep.2025.102618","url":null,"abstract":"<div><div>The present study investigated the effects of supplementing <em>Macleaya cordata</em> extract (MCE) in low-fishmeal or low-protein diet on the growth performance, blood biochemical indices, intestinal morphology, and intestinal microbiota of juvenile largemouth bass. Two control diets were prepared: a low-protein diet (LP-0) containing 360 g/kg fishmeal and 460 g/kg crude protein, and a low-fishmeal diet (LF-0) containing 270 g/kg fishmeal and 500 g/kg crude protein. Then, 500 mg/kg MCE (Sangrovit) was added to the above two diets resulting in LP-500 and LF-500 diets, respectively. Juvenile largemouth bass (initial weight 21.0 ± 1.0 g) were fed the four diets for 60 days. Results showed that supplementing 500 mg/kg MCE in the low-protein diet, but not in the low-fishmeal diet, significantly increased the weight gain of largemouth bass (<em>P</em> &lt; 0.05). In <em>Aeromonashydrophila</em> challenge test, the LP-500 group showed higher survival than the LP-0 group.In serum indices, LP-500 group presented significantly lower activity of diamine oxidase than the LP-0 group(<em>P</em> &lt; 0.05). Supplementing MCE in low-fishmeal or low-protein diets significantly increased the abundance of <em>Firmicutes</em> in the intestinal microbiota (<em>P</em> &lt; 0.05). Additionally<em>,</em> the expression levels of the inflammatory factors IL-10 and TGF-β1 were reduced. In summary, supplementing MCE in low-protein diet significantly improved the weight gain (<em>P</em> &lt; 0.05), and MCE supplementation in low-protein or low-fishmeal diet could modulate the intestinal microbiota composition and reduce the inflammation factors expression in largemouth bass.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102618"},"PeriodicalIF":3.2,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143143092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary curcumin supplementation on the liver health of juvenile channel catfish (Ictalurus punctatus) fed high-starch diet","authors":"Qisheng Lu ,&nbsp;Yutong Zheng ,&nbsp;Yu Wang ,&nbsp;Yulong Liu ,&nbsp;Jingyue Cao ,&nbsp;Haokun Liu ,&nbsp;Junyan Jin ,&nbsp;Zhimin Zhang ,&nbsp;Yunxia Yang ,&nbsp;Xiaoming Zhu ,&nbsp;Shouqi Xie ,&nbsp;Dong Han","doi":"10.1016/j.aqrep.2025.102615","DOIUrl":"10.1016/j.aqrep.2025.102615","url":null,"abstract":"<div><div>High-carbohydrate diet (HCD) usually leads to metabolic disorders and damages liver health in aquatic animals. Curcumin has been shown to improve antioxidant, hypoglycemic, and hypolipidemic effects. However, whether curcumin helps to ameliorate HCD-induced liver damage in fish remains unknown. Here, we formulated four diets: normal-carbohydrate diet (CON), high-starch diets (HSD), 0.02 % and 0.04 % curcumin added to HSD (HSC0.02 % and HSC0.04 %) to investigate the effects of curcumin on the liver health of channel catfish (<em>Ictalurus punctatus</em>). The results showed that HSC0.02 % or HSC0.04 % treatments had no effect on growth performance and HSC0.02 % treatment showed the best hypoglycemic and hypolipidemic effects compared to the HSD group. Dietary curcumin activated Keap1/Nrf2 to increase hepatic antioxidant capacity (T-AOC, SOD and GSH-PX) and inhibit apoptosis pathway (Bax, Bcl2, Caspase 3 and Caspase 9) (<em>P</em> &lt; 0.05). Importantly, curcumin reduces HSD-induced abnormalities of pentose phosphate pathway (PPP), triglyceride and cholesterol synthesis by inhibiting carbohydrate-responsive element-binding protein (ChREBP) expression (<em>P</em> &lt; 0.05). Thus, we elucidated that curcumin ameliorates HSD-induced metabolic dysfunction and damage by inhibiting ChREBP signaling in channel catfish and assessed the feasibility of curcumin as an aquafeed additive to improve liver health in aquatic animals.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102615"},"PeriodicalIF":3.2,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Positive effects of optimal dietary protein content on the growth, reproductive performance and health of Procambarus clarkii broodstock farming in rice field","authors":"Hui Luo ,&nbsp;Feng Qin ,&nbsp;Yu Li ,&nbsp;Chuanbo Xiao ,&nbsp;Wenbo Sun ,&nbsp;Fan Liu ,&nbsp;Guangjun Lv ,&nbsp;Hua Ye ,&nbsp;Yuanfa He ,&nbsp;Wenping He ,&nbsp;Feng Xu ,&nbsp;Yong Lin ,&nbsp;Dapeng Wang ,&nbsp;Honghao Jin","doi":"10.1016/j.aqrep.2024.102610","DOIUrl":"10.1016/j.aqrep.2024.102610","url":null,"abstract":"<div><div>This study was conducted to evaluate the optimal protein requirement of <em>Procambarus clarkii</em> cultured in paddy field, six isoenergetic and isolipid diets were formulated to contain graded levels of 28 %, 31 %, 34 %, 37 %, 40 % and 43 % crude protein (called P28–43 group). There were 3 replicates in each treatment and 20 crayfish in each replicate with an initial average body weight of (20.30 ± 0.24) g were fed for 56 days. Our results showed that final body weight was maximized in the P31 group, the weight gain rate and specific growth rate were maximized in the P34 group, all of which were significantly higher than those of P28 group (<em>P</em> &lt; 0.05). Significant improvements in reproductive index, fecundity, egg diameter and egg production were found by increasing the dietary protein level from 28 % to 37 % (<em>P</em> &lt; 0.05), while no significant increase was observed when the protein level was further increased to 43 % (<em>P</em> &gt; 0.05). Total essential amino acids, total non-essential amino acids, and total amino acids in muscle were not significantly affected by dietary protein content, all of which were lowest in P28 group. Muscle protein content significantly increased as dietary protein level increasing and was highest in P37 group (<em>P</em> &lt; 0.05). There was no significant difference in muscle moisture, crude lipid and crude ash content of among all groups (<em>P</em> &gt; 0.05). P43 group showed the maximum levels of aspartate transaminase activity, with a significant difference (<em>P</em> &lt; 0.05) compared to P31–37 groups. A positive correlation was observed between the dietary protein level and total bilirubin concentration. Dietary protein level had a significant impact on trypsin activity, with the P43 group showing significantly increased trypsin activity compared to the P28 group (<em>P</em> &lt; 0.05). The activity of total superoxide dismutase, catalase, glutathione peroxidase, and lysozyme peaked in the P31 group and was significantly higher than in the P43 group (<em>P</em> &lt; 0.05). The nitric oxide concentration was significantly higher in the P37 group compared to P28–34 and P43 groups (<em>P</em> &lt; 0.05). The malondialdehyde content significantly increased as dietary protein level increasing, which was highest in the P43 group (<em>P</em> &lt; 0.05). In conclusion, dietary protein level of 34.00 % provided superior growth performance for the <em>P. clarkii</em> broodstock, and the diets with 37.00 % protein were optimal for reproductive performance of <em>P. clarkii</em> broodstock in rice-crayfish culture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102610"},"PeriodicalIF":3.2,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143143093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of dietary mulberry (Morus alba) fruit extract on the growth performance, skin color, and immune response of fancy carp (Cyprinus carpio)","authors":"Wassana Prisingkorn ,&nbsp;Prasoborn Rinthong ,&nbsp;Narong Kamolrat ,&nbsp;Pattama Wiriyapattanasub ,&nbsp;Sutee Wongmaneeprateep ,&nbsp;Jariyavadee Suriyaphan ,&nbsp;Apichet Pholoeng ,&nbsp;Eakapol Wangkahart","doi":"10.1016/j.aqrep.2024.102611","DOIUrl":"10.1016/j.aqrep.2024.102611","url":null,"abstract":"<div><div>Supplementing aquafeed with functional ingredients can improve fish immunity, growth performance, and survival rates. <em>Morus alba</em> var. Chiangmai is a medicinal plant recognized for its antioxidant, anti-inflammatory, and antimicrobial properties, and it is rich in flavonoids, including rutin, quercetin, kaempferol, and anthocyanins. The present study aimed to investigate the effects of Mulberry (Mull) fruit extract on the growth performance, coloration, immune response, gene expression, and liver histology of fancy carp (<em>Cyprinus carpio</em>). We conducted an 8-week growth trial on fancy carp using four different dietary groups: commercial, control, Mull1 %, and Mull2 %. The results showed that fish fed Mull1 % exhibited improved growth performance (FBW, WG, ADG, FCR) compared to control and Mull2 % groups (<em>P</em> &lt; 0.05), although survival rates did not differ significantly among groups (<em>P</em> &gt; 0.05). Color analysis (CIE <em>Lab</em>*) was conducted at three positions on the fish body (dorsal fin, pectoral fin, and caudal fin). The results revealed that fish on the Mull1 % diet had significantly increased red (<em>a</em>*) and yellow (<em>b</em>*) coloration at the dorsal fin and caudal fin positions, without affecting lightness (<em>L</em>*) (<em>P</em> &lt; 0.05). In contrast, Mull2 % diet had no significant impact on these color parameters (<em>P</em> &gt; 0.05). Immunological evaluation showed that Mull1 % enhanced key immune markers such as CAT, LZM, MPO, GPx, and GRD while reducing MDA content in the liver (<em>P</em> &lt; 0.05), suggesting an improved immune response. Gene expression analysis indicated upregulation of <em>il-1β</em>, <em>cat</em>, and <em>vtg</em> in the Mull1 % group, while <em>hsp70</em> expression was highest in the Mull2 % group, highlighting a dose-dependent effect on immune and stress response genes (<em>P</em> &lt; 0.05). Liver histology revealed a normal structure across all groups, indicating no adverse effects from the diets, including those with mulberry supplementation. In conclusion, 1 % mulberry extract supplementation improved growth, coloration, immune response, and gene expression in fancy carp without negatively affecting liver histology, making it a promising dietary additive for ornamental fish.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102611"},"PeriodicalIF":3.2,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N-acetylcysteine increased the growth performance of Leuciscus waleckii by improving mitochondrial function, enhancing antioxidant and anti-inflammatory, and inhibiting apoptosis in the hepatopancreas and intestine","authors":"Deng Lai Li ,&nbsp;Rui Zhu ,&nbsp;Si Tong Meng ,&nbsp;Yin Tao Wang ,&nbsp;Si Ying Liu ,&nbsp;Zhi Yong Yang ,&nbsp;Li Fang Wu","doi":"10.1016/j.aqrep.2024.102613","DOIUrl":"10.1016/j.aqrep.2024.102613","url":null,"abstract":"<div><div>This experiment investigated the effects of N-Acetylcysteine (NAC) addition to diets on growth performance, mitochondrial function, antioxidant capacity, immune function and apoptosis in <em>Leuciscus waleckii</em>. Diets containing different levels of NAC (0.00 g/kg, 1.50 g/kg, 3.00 g/kg, 4.50 g/kg, 6.00 g/kg) were formulated. An 8-week feeding trial was conducted with these diets. The results revealed that 3 g/kg-6 g/kg NAC increased FW, WGR, SGR, PER, and FER and improved growth performance and feed utilization in <em>Leuciscus waleckii</em>. 4.50 g/kg-6.00 g/kg NAC increased the intestinal and hepatopancreatic digestive enzyme activities, enhancing digestive function. 3.00 g/kg-4.50 g/kg NAC increased serum ACP and AKP activities, decreased IL-1β and TNF-α levels, and optimized serum biochemical indices in Leuciscus waleckii. Appropriate level of NAC upregulated mRNA expression of <em>PPARγ</em>, ATGL and CPT-1, and HDL-C, CPT-1, ATGL and FAS activities to promote lipid metabolism. 3.00 g/kg-6.00 g/kg NAC promotes hepatopancreatic and intestinal anti-inflammatory and antioxidant capacities by activating Nrf2 and inhibiting the NF-κB signaling pathway. NAC supplementation also maintains mitochondrial membrane potential and increases ATP synthase activities and ATP content, thereby improving hepatopancreatic and intestinal mitochondrial function in <em>Leuciscus waleckii</em>. Meanwhile, 4.50 g/kg-6.00 g/kg NAC reduced the mRNA expression of hepatopancreatic and intestinal <em>Bax</em>, <em>Caspase-3</em> and <em>Caspase-9</em>, and Caspase-3, Caspase-8 and Caspase-9 enzyme activities, and inhibited apoptosis. NAC supplementation upregulates the mRNA expression of <em>Claudin-3</em>, <em>Claudin-f</em>, <em>ZO-1</em>, and <em>Occludin-a</em> and reduces intestinal permeability-related markers, thereby maintaining intestinal physical barrier function. HE staining results showed that NAC maintained the morphological and structural integrity of the hepatopancreas and intestine. In conclusion, dietary supplementation with 4.50 g/kg NAC promotes the growth of <em>Leuciscus waleckii</em> by improving mitochondrial function, enhancing antioxidant and anti-inflammatory effects, inhibiting apoptosis, and improving hepatopancreatic and intestinal health.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102613"},"PeriodicalIF":3.2,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptome analysis reveals potential regulatory mechanisms in response to changes in physiological functions in Oreochromis aureus under salinity stress","authors":"Chang Yuan ,&nbsp;Kangqi Zhou ,&nbsp;Xianhui Pan,&nbsp;Yong Lin,&nbsp;Junqi Qin,&nbsp;Dapeng Wang,&nbsp;Zhong Chen,&nbsp;Xuesong Du,&nbsp;Yin Huang","doi":"10.1016/j.aqrep.2024.102608","DOIUrl":"10.1016/j.aqrep.2024.102608","url":null,"abstract":"<div><div><em>Oreochromis aureus</em> is an economically valuable fish species, but its domestication in saline environments remains unexplored, with limited reports on how salinity stress affects its physiological functions and acclimation-related mechanisms. Thus, this study collected <em>O. aureus</em> exposed to different salinity stress levels (0 ‰, 3 ‰, 7 ‰, and 11 ‰) and analyzed their growth performance, histopathology, physiological functions, and transcriptome. The results showed that salinity stress had no significant effect on growth performance. Salinity stress damaged gill tissues, decreased physiological and antioxidant activities, increased osmotic and antimicrobial activities, and altered digestive functions. Comparative transcriptome analyses identified 38,910 differentially expressed genes (DEGs), of which 11,488 were common to the three comparisons. These DEGs were significantly associated with specific salinity stress response-related KEGG pathways, including Sphingolipid signaling pathway, Lysosome, Phagosome, and Focal adhesion. The present results identify 1 GO term (regulation of biological proces) in response to salinity stress. Furthermore, 15 candidate genes related to salinity stress responses and physiological functions were also identified. (e.g., <em>TLR2</em>, <em>NCF2</em>, <em>Sptlc2</em>, and <em>ctsd</em>). On the basis of GO, KEGG and STEM analyses, the data enabled the development of a mechanistic model that details how <em>O. aureus</em> adapts to salinity stress by regulating physiological changes. Finally, RT-qPCR assays verified the accuracy and reliability of the high-throughput sequencing results. This study enhances our understanding of <em>O. aureus</em> adaptive strategies under salinity stress, while also providing relevant theoretical insights into the domestication of fish under saline conditions and the mechanisms mediating adaptations to saline aquatic environments.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102608"},"PeriodicalIF":3.2,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardization of breeding and rearing of the endangered Indian catfish Clarias magur (Hamilton, 1822) for juvenile production","authors":"Kamal Sarma ,&nbsp;Bavithra R. ,&nbsp;Jaspreet Singh ,&nbsp;S.K. Ahirwal ,&nbsp;Ujjwal Kumar ,&nbsp;Tarkeshwar Kumar ,&nbsp;Maneesh Kumar Dubey","doi":"10.1016/j.aqrep.2024.102600","DOIUrl":"10.1016/j.aqrep.2024.102600","url":null,"abstract":"<div><div>The walking catfish, <em>Clarias magur</em>, is one of India's most popular food fish because of its nutritional quality and taste. In recent times, more attention has been given to this species due to the alarmingly decreasing availability in natural water bodies. Breeding and seed rearing of this species are still bottlenecks to an uninterrupted seed supply. A study was initiated to standardize the breeding performance of this species using the WOVA-FH hormone. Four hormone doses were selected (0.5, 1.0, 1.5 and 2.0 ml/kg body weight of female), and results indicated that the total weight of stripped eggs and spawning fecundity were the highest (p &lt; 0.05) when females were injected with 1.0 ml of WOVA-FH hormone per kg of body weight. In the rearing experiment, the weight gain of larvae obtained from fishes injected with different hormone doses did not differ significantly (p &gt; 0.05). Average larval growth at the end of 30, 60, 90 and 120 days of rearing was 0.003 g, 0.085 g, 4.47 g, 11.77 g and 23.77 g, respectively. For juvenile production from the fingerlings of <em>C. magur</em>, different protein levels (20 %, 25 %, 30 %, 35 % and 40 %) of feed were prepared from locally available feed ingredients, and the growth and survival performance of <em>C. magur</em> under varying protein levels were recorded. Results indicated that <em>C. magur</em> required relatively higher protein feed (35 %) for better growth performance, and varying protein level has a direct impact on the growth, survival, mineral and proximate composition of <em>C. magur.</em></div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102600"},"PeriodicalIF":3.2,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated metabolomics and proteomics analysis to provide insights into muscle atrophy of turbot Scophthalmus maximus by dietary Clostridium autoethanogenum protein","authors":"Shihui Pan ,&nbsp;Nan Bai ,&nbsp;Zezheng Qi ,&nbsp;Chuwen Chen ,&nbsp;Daisong Ma ,&nbsp;Zhenlin Liang ,&nbsp;Min Gu","doi":"10.1016/j.aqrep.2024.102603","DOIUrl":"10.1016/j.aqrep.2024.102603","url":null,"abstract":"<div><div><em>Clostridium autoethanogenum</em> protein (CAP) is a promising protein source for aquaculture. Previous studies proved that high levels of dietary CAP retarded the muscle development in turbot through mammalian target of rapamycin (mTOR)/amino acid response (AAR)/adenosine monophosphate-activated protein kinase (AMPK) pathways. However, how CAP regulating these pathways was still unclear and there was no information on the comprehensive analysis for the mechanisms of CAP on fish muscle. In the present study, the integration of metabolomic and proteomic was applied on the muscle of turbot (initial weight of 150 ± 3 g) fed with fishmeal-based diet or diet with 60 % of fishmeal protein replaced by CAP for 70 days. The present work confirmed that CAP decreased the growth, fillet yield and induced the muscle atrophy. Integrated analysis of metabolomics and proteomics showed that CAP negatively influenced the energy production by inhibiting the key enzymes of glycolysis and mitochondrial respiratory chain. Second, CAP inhibited mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3-kinase (PI3K) pathways to down-regulate mTOR pathways and protein synthesis. Third, the amino acid contents in turbot muscle were imbalanced by CAP, which would be perceived by general control nonderepressible 1 in AAR signal pathways. Finally, the up-regulated AAR induced amino acids catabolism through purine-based combined deamination. In conclusion, the present work provided the universal knowledge on the working principle of CAP and laid the foundation for CAP improvement in the future.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"40 ","pages":"Article 102603"},"PeriodicalIF":3.2,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}