Pub Date : 2025-10-03DOI: 10.1016/j.aqrep.2025.103133
Bruna Petani , Ivan Župan , Simone Mirto , Elena Manini , Anamaria Baričević , Bosiljka Mustać , Francesca Ape
This investigation aimed to analyze for the first time the impact of fish farming on the biochemical composition of sedimentary organic matter and meiofaunal assemblages on a sea bass/sea bream farm located in the central Croatian waters. We compared two aquaculture sites: one near the cages (ST 1) and one directly beneath them (ST 2), along with a control site (Control) located 700 m away. Our findings revealed significant organic matter enhancement in the sediments surrounding the cages, leading to increased total meiofaunal abundance but decreased diversity. Furthermore, meiofaunal community structure differed among aquaculture and control sediments, particularly in terms of rare taxa (less than 1 % abundance). Increased Nematoda abundances contrasted with the decline or absence of more sensitive taxa like Copepoda and Kinorhyncha near the fish farm. These results highlight the importance of considering meiofaunal rare taxa, such as Kinorhyncha, for better understanding benthic responses to increased anthropogenic impacts and support the suggestion of other authors to use the nematodes to kinorhyncs ratio as a useful indicator of organic enrichment associated with aquaculture activities.
{"title":"The use of meiofauna as an indicator of fish-farms impact: a case study from the middle eastern Adriatic Sea","authors":"Bruna Petani , Ivan Župan , Simone Mirto , Elena Manini , Anamaria Baričević , Bosiljka Mustać , Francesca Ape","doi":"10.1016/j.aqrep.2025.103133","DOIUrl":"10.1016/j.aqrep.2025.103133","url":null,"abstract":"<div><div>This investigation aimed to analyze for the first time the impact of fish farming on the biochemical composition of sedimentary organic matter and meiofaunal assemblages on a sea bass/sea bream farm located in the central Croatian waters. We compared two aquaculture sites: one near the cages (ST 1) and one directly beneath them (ST 2), along with a control site (Control) located 700 m away. Our findings revealed significant organic matter enhancement in the sediments surrounding the cages, leading to increased total meiofaunal abundance but decreased diversity. Furthermore, meiofaunal community structure differed among aquaculture and control sediments, particularly in terms of rare taxa (less than 1 % abundance). Increased Nematoda abundances contrasted with the decline or absence of more sensitive taxa like Copepoda and Kinorhyncha near the fish farm. These results highlight the importance of considering meiofaunal rare taxa, such as Kinorhyncha, for better understanding benthic responses to increased anthropogenic impacts and support the suggestion of other authors to use the nematodes to kinorhyncs ratio as a useful indicator of organic enrichment associated with aquaculture activities.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103133"},"PeriodicalIF":3.7,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-03DOI: 10.1016/j.aqrep.2025.103131
Petra Drzewnioková , Romy Lucon Xiccato , Francesca Bruno , Claudia Casarotto , Matteo Mazzucato , Eleonora Franzago , Marica Toson , Niccolò Vendramin , Anna Toffan
Piscine orthoreovirus genotype 3 (PRV-3) has been reported in several European countries, and in Italy it was detected only once in 2017 without any associated disease outbreak. This study aimed to investigate the presence of PRV-3 in the area with the highest salmonids production in Italy. Samples were collected between September 2023 and May 2024 from three regions of northeastern Italy. Pooled samples were screened by a generic PRV real-time RT-PCR, and positive samples were subsequently characterized as PRV-3. The geographic distribution of PRV-3 was visualised and risk factors analysis was performed. Of the 267 tested specimens, 37.1 % yielded positive results, corresponding to 43.6 % positive farms in the investigated area. Phylogenetic analyses confirmed that the Italian PRV-3 strains clustered within group 3b. The highest positivity was observed in Salmo trutta (49.1 %), followed by Oncorhynchus mykiss (38.7 %) and Salmo marmoratus (24.3 %). No signs of diseases or history of mortality were observed from the positive batches of fish. Apparently healthy wild fish also tested positive. These results suggest that PRV-3 is widespread in both farmed and wild salmonid populations in the investigated area and that it does not cause specific health issues under the current Italian farming conditions and wild environment.
{"title":"Field surveillance has revealed a high prevalence of PRV-3 in clinically healthy salmonids in Italy","authors":"Petra Drzewnioková , Romy Lucon Xiccato , Francesca Bruno , Claudia Casarotto , Matteo Mazzucato , Eleonora Franzago , Marica Toson , Niccolò Vendramin , Anna Toffan","doi":"10.1016/j.aqrep.2025.103131","DOIUrl":"10.1016/j.aqrep.2025.103131","url":null,"abstract":"<div><div>Piscine orthoreovirus genotype 3 (PRV-3) has been reported in several European countries, and in Italy it was detected only once in 2017 without any associated disease outbreak. This study aimed to investigate the presence of PRV-3 in the area with the highest salmonids production in Italy. Samples were collected between September 2023 and May 2024 from three regions of northeastern Italy. Pooled samples were screened by a generic PRV real-time RT-PCR, and positive samples were subsequently characterized as PRV-3. The geographic distribution of PRV-3 was visualised and risk factors analysis was performed. Of the 267 tested specimens, 37.1 % yielded positive results, corresponding to 43.6 % positive farms in the investigated area. Phylogenetic analyses confirmed that the Italian PRV-3 strains clustered within group 3b. The highest positivity was observed in <em>Salmo trutta</em> (49.1 %), followed by <em>Oncorhynchus mykiss</em> (38.7 %) and <em>Salmo marmoratus</em> (24.3 %). No signs of diseases or history of mortality were observed from the positive batches of fish. Apparently healthy wild fish also tested positive. These results suggest that PRV-3 is widespread in both farmed and wild salmonid populations in the investigated area and that it does not cause specific health issues under the current Italian farming conditions and wild environment.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103131"},"PeriodicalIF":3.7,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-01DOI: 10.1016/j.aqrep.2025.103121
Liang Wu , Xiaoli Shi , Jian Wang , Xiafei Zheng , Xueqiao Qian , Shouqi Xie , Baojun Shi , Ruiming Xiao , Bo Wang , Yingli Lian , Jiayi Tang
Aquatic microbiota plays crucial roles in biogeochemical processes. However, whether the water nutrients and co-occurrence of sediment microbiota in white-leg shrimp culture ponds increases during culture is unclear. We investigated the water nutrients and sediment microbiota in six white-leg shrimp culture ponds during culture using high-throughput 16S rRNA gene sequencing. Our results showed that water total nitrogen (TN), NO3-N, NO2-N, and total phosphorus were typically increased during the white-leg shrimp culture process. Although the overall structure of the sediment microbiota in the ponds was not altered during culture, the relative abundance of Caldicoprobacter, Dechloromonas, Erysipelothrix, Hydrogenophaga, and Mariniradius gradually decreased. Particularly, the co-occurrence of sediment microbiota among the different ponds was enhanced during the culture process. The concentrations of TN, NO3-N, and total organic carbon in the water were significantly positively correlated with the average weighted degree, average clustering coefficient, and total triangles of the co-occurrence networks of the sediment microbiota. These results imply the accumulation of nutrients in the pond water probably promoted the co-occurrence of sediment microbiota, which implying the convergence of the sediment microbiota across ponds. Therefore, microbial cooccurrence has potential to be an index to measure the nutritional status of aquaculture ponds.
{"title":"Elevated water nutrient levels promote co-occurrence of sediment microbiota in white-leg shrimp (Litopenaeus vannamei) culture ponds","authors":"Liang Wu , Xiaoli Shi , Jian Wang , Xiafei Zheng , Xueqiao Qian , Shouqi Xie , Baojun Shi , Ruiming Xiao , Bo Wang , Yingli Lian , Jiayi Tang","doi":"10.1016/j.aqrep.2025.103121","DOIUrl":"10.1016/j.aqrep.2025.103121","url":null,"abstract":"<div><div>Aquatic microbiota plays crucial roles in biogeochemical processes. However, whether the water nutrients and co-occurrence of sediment microbiota in white-leg shrimp culture ponds increases during culture is unclear. We investigated the water nutrients and sediment microbiota in six white-leg shrimp culture ponds during culture using high-throughput 16S rRNA gene sequencing. Our results showed that water total nitrogen (TN), NO<sub>3</sub>-N, NO<sub>2</sub>-N, and total phosphorus were typically increased during the white-leg shrimp culture process. Although the overall structure of the sediment microbiota in the ponds was not altered during culture, the relative abundance of <em>Caldicoprobacter</em>, <em>Dechloromonas</em>, <em>Erysipelothrix</em>, <em>Hydrogenophaga</em>, and <em>Mariniradius</em> gradually decreased. Particularly, the co-occurrence of sediment microbiota among the different ponds was enhanced during the culture process. The concentrations of TN, NO<sub>3</sub>-N, and total organic carbon in the water were significantly positively correlated with the average weighted degree, average clustering coefficient, and total triangles of the co-occurrence networks of the sediment microbiota. These results imply the accumulation of nutrients in the pond water probably promoted the co-occurrence of sediment microbiota, which implying the convergence of the sediment microbiota across ponds. Therefore, microbial cooccurrence has potential to be an index to measure the nutritional status of aquaculture ponds.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103121"},"PeriodicalIF":3.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-01DOI: 10.1016/j.aqrep.2025.103135
Antigoni Vasilaki , Ioannis Nengas , Dimitra Kogiannou , Morgane Henry , Chrysanthi Nikoloudaki , Petros Chronopoulos , Panagiotis Berillis , Eleni Golomazou , Eleni Fountoulaki , Ioannis T. Karapanagiotidis , Elena Mente
The aim of the current study was to evaluate the nutritional value of Lupinus albus processed with exogenous enzymes as replacer of soybean meal. Six hundred European sea bass (11.2 ± 0.0 g) were distributed in 12 tanks and fed the 4 experimental diets containing soybean meal to processed lupin meal at ratios: Control (15 %: 0 %), Lupin 1 (5 %: 7.5 %), Lupin 2 (2.5 %: 10 %), Lupin 3 (0 %: 12.5 %) in triplicate groups for 83 d. The results showed significantly higher final body weight (P = 0.050) and specific growth rate (P = 0.036) and lower feed conversion ratio (P = 0.047) for the group fed the Lupin 1 diet compared to the Control diet. Significantly higher apparent digestibility coefficient of crude protein (P = 0.043) was noted for the group tested with the Lupin 3 diet compared to Control and apparent digestibility coefficient of fat was significantly higher for groups fed the Lupin 1 (P = 0.015) and Lupin 3 (P = 0.000) diets compared to Control. Essential amino acid deposition was higher for the group fed the Lupin 2 diet related to the Control group and the same was observed for trypsin activity in the pyloric caeca (P = 0.031) and intestine (P = 0.016) of fish. Positive effect was observed in the immune status of fish fed the processed lupin meal and no histopathological alterations were determined. Comprehensively, the results proved the potential of processed lupin meal at 12.5 % to totally replace soybean meal in feed formulation for Mediterranean aquaculture marine species.
{"title":"Evaluation of the nutritional value of processed lupin meal (Lupinus albus) with exogenous enzymes, as feed ingredient in European sea bass (Dicentrarchus labrax) aquafeeds","authors":"Antigoni Vasilaki , Ioannis Nengas , Dimitra Kogiannou , Morgane Henry , Chrysanthi Nikoloudaki , Petros Chronopoulos , Panagiotis Berillis , Eleni Golomazou , Eleni Fountoulaki , Ioannis T. Karapanagiotidis , Elena Mente","doi":"10.1016/j.aqrep.2025.103135","DOIUrl":"10.1016/j.aqrep.2025.103135","url":null,"abstract":"<div><div>The aim of the current study was to evaluate the nutritional value of <em>Lupinus albus</em> processed with exogenous enzymes as replacer of soybean meal. Six hundred European sea bass (11.2 ± 0.0 g) were distributed in 12 tanks and fed the 4 experimental diets containing soybean meal to processed lupin meal at ratios: Control (15 %: 0 %), Lupin 1 (5 %: 7.5 %), Lupin 2 (2.5 %: 10 %), Lupin 3 (0 %: 12.5 %) in triplicate groups for 83 d. The results showed significantly higher final body weight (<em>P</em> = 0.050) and specific growth rate (<em>P</em> = 0.036) and lower feed conversion ratio (<em>P</em> = 0.047) for the group fed the Lupin 1 diet compared to the Control diet. Significantly higher apparent digestibility coefficient of crude protein (<em>P</em> = 0.043) was noted for the group tested with the Lupin 3 diet compared to Control and apparent digestibility coefficient of fat was significantly higher for groups fed the Lupin 1 (<em>P</em> = 0.015) and Lupin 3 (<em>P</em> = 0.000) diets compared to Control. Essential amino acid deposition was higher for the group fed the Lupin 2 diet related to the Control group and the same was observed for trypsin activity in the pyloric caeca (<em>P</em> = 0.031) and intestine (<em>P</em> = 0.016) of fish. Positive effect was observed in the immune status of fish fed the processed lupin meal and no histopathological alterations were determined. Comprehensively, the results proved the potential of processed lupin meal at 12.5 % to totally replace soybean meal in feed formulation for Mediterranean aquaculture marine species.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103135"},"PeriodicalIF":3.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-01DOI: 10.1016/j.aqrep.2025.103132
Yue-Miao Yu , Ke-Xin Yu , Li Sun , Dong-Ji Song , Da-Yan Hu , Shao-Kui Yi , Yang Li
The neuropeptide F receptor (NPFR) is a critical regulator of energy homeostasis in invertebrates, orchestrating feeding behavior, nutrient assimilation, and reproductive functions. We report the successful cloning and functional characterization of NPFR from the giant freshwater prawn Macrobrachium rosenbergii. Structural analysis confirmed that the cloned sequence is a typical GPCR with seven transmembrane domains and conserved motifs DRY and NPLLY. Sequence alignment showed high homology (>90 %) with other long-arm shrimp species, supporting evolutionary conservation within the Palaemonidae family. Quantitative real-time PCR (qRT-PCR) revealed ubiquitous expression of mrNPFR, with the highest levels in the intestinal tract, followed by hemolymph and eyestalk ganglia, suggesting multifunctional roles in energy homeostasis and neuroendocrine regulation. Functional assays using luciferase reporter systems demonstrated that NPF-II was the most potent cAMP inhibitor, while NPF-III exhibited the highest efficacy in activating the MAPK/ERK pathway. NPF-I showed the highest activation efficacy for the NFAT pathway. This study provides foundational data for understanding NPFR’s physiological functions in M. rosenbergii and offers insights for precision feeding strategies in aquaculture.
{"title":"Pharmacological characterization of the neuropeptide F receptor (NPFR) in Macrobrachium rosenbergii","authors":"Yue-Miao Yu , Ke-Xin Yu , Li Sun , Dong-Ji Song , Da-Yan Hu , Shao-Kui Yi , Yang Li","doi":"10.1016/j.aqrep.2025.103132","DOIUrl":"10.1016/j.aqrep.2025.103132","url":null,"abstract":"<div><div>The neuropeptide F receptor (NPFR) is a critical regulator of energy homeostasis in invertebrates, orchestrating feeding behavior, nutrient assimilation, and reproductive functions. We report the successful cloning and functional characterization of NPFR from the giant freshwater prawn <em>Macrobrachium rosenbergii</em>. Structural analysis confirmed that the cloned sequence is a typical GPCR with seven transmembrane domains and conserved motifs DRY and NPLLY. Sequence alignment showed high homology (>90 %) with other long-arm shrimp species, supporting evolutionary conservation within the <em>Palaemonidae</em> family. Quantitative real-time PCR (qRT-PCR) revealed ubiquitous expression of mrNPFR, with the highest levels in the intestinal tract, followed by hemolymph and eyestalk ganglia, suggesting multifunctional roles in energy homeostasis and neuroendocrine regulation. Functional assays using luciferase reporter systems demonstrated that NPF-II was the most potent cAMP inhibitor, while NPF-III exhibited the highest efficacy in activating the MAPK/ERK pathway. NPF-I showed the highest activation efficacy for the NFAT pathway. This study provides foundational data for understanding NPFR’s physiological functions in <em>M. rosenbergii</em> and offers insights for precision feeding strategies in aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103132"},"PeriodicalIF":3.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We experimentally tested the effects of on-site biofouling removal (BR) via heat treatment on the biomolecule contents of Pacific oysters and the bay-scale recovery of these biomolecules through the harvest of farmed oysters from suspension aquaculture in a temperate bay. At harvest after an 18-month cultivation period, oysters treated at the 13th month showed 18 % higher soft body weights and increased contents of certain biomolecules compared to untreated (UN) oysters. Specifically, BR oysters had 15 % higher eicosapentaenoic acid (EPA) content and 13 % higher docosahexaenoic acid (DHA) content than UN oysters. The glycogen content of BR oysters was 54 % higher, suggesting enhanced energy storage and potentially improved sweetness. BR likely reduced food competition between oysters and fouling organisms, predominantly mussels, leading to the higher biomolecule contents. If BR were expanded to cover the entire bay (from the current 10–100 %), the recovery of EPA and DHA through the harvest of farmed oysters would increase by 28 %, reaching 869 kg in a 1.5-year production cycle. Biofouling removal (BR) did not significantly affect the content of essential amino acids (AAs), gustatory-related AAs, or taurine in oysters. However, due to improved oyster biomass production, BR is estimated to potentially increase the recovery of these biomolecules by approximately 12 %. In conclusion, on-site BR is a straightforward and effective method for enhancing the levels of certain nutritional and gustatory-related biomolecules in cultivated oysters while simultaneously increasing the recovery of nutritionally important biomolecules for human consumption.
{"title":"On-site biofouling removal enhances the nutritional and gustatory quality of oysters from suspension aquaculture and the recovery of essential biomolecules for human consumption","authors":"Yuji Hatakeyama , Chikako Maruo , Megumu Fujibayashi , Ramon Filgueira , Takashi Sakamaki","doi":"10.1016/j.aqrep.2025.103129","DOIUrl":"10.1016/j.aqrep.2025.103129","url":null,"abstract":"<div><div>We experimentally tested the effects of on-site biofouling removal (BR) via heat treatment on the biomolecule contents of Pacific oysters and the bay-scale recovery of these biomolecules through the harvest of farmed oysters from suspension aquaculture in a temperate bay. At harvest after an 18-month cultivation period, oysters treated at the 13th month showed 18 % higher soft body weights and increased contents of certain biomolecules compared to untreated (UN) oysters. Specifically, BR oysters had 15 % higher eicosapentaenoic acid (EPA) content and 13 % higher docosahexaenoic acid (DHA) content than UN oysters. The glycogen content of BR oysters was 54 % higher, suggesting enhanced energy storage and potentially improved sweetness. BR likely reduced food competition between oysters and fouling organisms, predominantly mussels, leading to the higher biomolecule contents. If BR were expanded to cover the entire bay (from the current 10–100 %), the recovery of EPA and DHA through the harvest of farmed oysters would increase by 28 %, reaching 869 kg in a 1.5-year production cycle. Biofouling removal (BR) did not significantly affect the content of essential amino acids (AAs), gustatory-related AAs, or taurine in oysters. However, due to improved oyster biomass production, BR is estimated to potentially increase the recovery of these biomolecules by approximately 12 %. In conclusion, on-site BR is a straightforward and effective method for enhancing the levels of certain nutritional and gustatory-related biomolecules in cultivated oysters while simultaneously increasing the recovery of nutritionally important biomolecules for human consumption.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103129"},"PeriodicalIF":3.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-30DOI: 10.1016/j.aqrep.2025.103117
Roberto González-Garoz , Almudena Cabezas , Elisabet González de Chávarri , Montserrat Fernández-Muela , Andrea Martínez Villalba , Morris Villarroel , Álvaro de la Llave-Propín , Jesús de la Fuente , Rubén Bermejo-Poza , María Teresa Díaz
This study compared live chilling (LC) and dry electrical stunning at higher (HI: 400 mA for 0.5 s followed by 200 mA for 1.5 s) and lower (LI: 200 mA for 2 s) intensities in combination with live chilling in rainbow trout (Oncorhynchus mykiss), evaluating their impact on muscle damage, metabolism, and sensory attributes under winter and summer. Trout were stunned, slaughtered and samples were taken for biochemical markers, muscle pH, rigor mortis, texture, color and freshness analysis. Our results showed that stunning method and seasonal conditions significantly influenced product quality, with low-intensity electrical stunning being most effective under warmer conditions. Blood levels of lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) were highest in LC and HI groups, indicating greater muscle damage, compared to LI. Osmolality was also elevated in HI, suggesting increased cell damage. Seasonality influenced muscle metabolism: summer results, compared with winter, showed lower glycogen reserves and reduced glycolytic enzyme activity (lower HK, PK, LDH activity), along with increased gluconeogenesis (higher FBP activity), and also altered oxidative stress, evidenced by up-regulated sod and gst gene expression and down-regulated gpx and cat expression in muscle. Muscle pH at 0 h post-mortem was lowest in LC, while rigor mortis developed faster in this group in summer. Texture analysis showed an interaction between stunning method and season, with slightly higher cohesiveness in LI group in summer. Seasonal effects on texture included lower hardness and springiness but higher adhesiveness in summer. Despite differences, no significant variations in color stability or freshness between stunning methods were observed.
{"title":"Comparing stunning methods and seasonal effects on biochemical variables and product quality of rainbow trout","authors":"Roberto González-Garoz , Almudena Cabezas , Elisabet González de Chávarri , Montserrat Fernández-Muela , Andrea Martínez Villalba , Morris Villarroel , Álvaro de la Llave-Propín , Jesús de la Fuente , Rubén Bermejo-Poza , María Teresa Díaz","doi":"10.1016/j.aqrep.2025.103117","DOIUrl":"10.1016/j.aqrep.2025.103117","url":null,"abstract":"<div><div>This study compared live chilling (LC) and dry electrical stunning at higher (HI: 400 mA for 0.5 s followed by 200 mA for 1.5 s) and lower (LI: 200 mA for 2 s) intensities in combination with live chilling in rainbow trout (<em>Oncorhynchus mykiss</em>), evaluating their impact on muscle damage, metabolism, and sensory attributes under winter and summer. Trout were stunned, slaughtered and samples were taken for biochemical markers, muscle pH, <em>rigor mortis</em>, texture, color and freshness analysis. Our results showed that stunning method and seasonal conditions significantly influenced product quality, with low-intensity electrical stunning being most effective under warmer conditions. Blood levels of lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) were highest in LC and HI groups, indicating greater muscle damage, compared to LI. Osmolality was also elevated in HI, suggesting increased cell damage. Seasonality influenced muscle metabolism: summer results, compared with winter, showed lower glycogen reserves and reduced glycolytic enzyme activity (lower HK, PK, LDH activity), along with increased gluconeogenesis (higher FBP activity), and also altered oxidative stress, evidenced by up-regulated <em>sod</em> and <em>gst</em> gene expression and down-regulated <em>gpx</em> and <em>cat</em> expression in muscle. Muscle pH at 0 h <em>post-mortem</em> was lowest in LC, while <em>rigor mortis</em> developed faster in this group in summer. Texture analysis showed an interaction between stunning method and season, with slightly higher cohesiveness in LI group in summer. Seasonal effects on texture included lower hardness and springiness but higher adhesiveness in summer. Despite differences, no significant variations in color stability or freshness between stunning methods were observed.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103117"},"PeriodicalIF":3.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-30DOI: 10.1016/j.aqrep.2025.103137
Haocheng Zhao , Mei Liu , Ziwen Ren , Keyong Jiang , Xudong Zhao , Kefeng Xu , Yan Gao , Baojie Wang , Lei Wang
Litopenaeus vannamei is a key economic species in global aquaculture, and monitoring its growth is critical for optimizing feed management, and reducing costs. Traditional manual sampling methods are labor-intensive, error-prone, and can cause stress or injury to shrimp, negatively impacting growth. This study employed computer vision and machine learning to propose an innovative approach for growth prediction and digestive tract assessment in L. vannamei. An improved annotation method was developed to simplify the marking process and reduce weight prediction errors. Furthermore, A new length measurement approach, termed “visual total length”, was introduced to overcome the limitations of traditional measurement techniques. In this study, images of shrimp on feeding trays were analyzed to simulate real aquaculture monitoring conditions, and a prediction system was constructed by combining image segmentation (You Only Look Once v8n-seg, YOLOv8n-seg), classification (YOLOv8n-cls), traditional fitting, and machine learning models (Light Gradient Boosting Machine, LightGBM). The results showed that the improved annotation method also significantly reduced weight prediction errors caused by tail fan area. The visual total length had a high linear correlation with traditional total length (r² = 0.99), effectively allowing it to replace traditional measurements and enhancing applicability in real production environments. The final model achieved an accuracy of over 97 % in predicting length and weight when compared to manual measurements, and over 87 % accuracy in assessing digestive tract fullness. This study provides an efficient and precise method for growth monitoring, laying a solid foundation in future intelligent shrimp aquaculture.
凡纳滨对虾（Litopenaeus vannamei）是全球水产养殖的重要经济物种，监测其生长对优化饲料管理和降低成本至关重要。传统的人工取样方法是劳动密集型的，容易出错，并且可能对虾造成压力或伤害，对生长产生负面影响。本研究利用计算机视觉和机器学习技术，提出了一种用于凡纳梅生长预测和消化道评估的创新方法。为了简化标注过程，降低权重预测误差，提出了一种改进的标注方法。此外，提出了一种新的长度测量方法，称为“视觉总长度”，以克服传统测量技术的局限性。本研究通过对虾在喂料盘上的图像进行分析，模拟真实养殖监测条件，并结合图像分割（You Only Look Once v8n-seg, YOLOv8n-seg）、分类（YOLOv8n-cls）、传统拟合和机器学习模型（Light Gradient Boosting machine, LightGBM）构建预测系统。结果表明，改进后的标注方法还显著降低了尾扇面积引起的权重预测误差。视觉总长度与传统总长度具有高度的线性相关性（r²= 0.99），有效地取代了传统测量方法，提高了在实际生产环境中的适用性。与人工测量相比，最终模型在预测长度和体重方面的准确性超过97% %，在评估消化道充实度方面的准确性超过87% %。本研究为对虾的生长监测提供了一种高效、精确的方法，为未来对虾的智能化养殖奠定了坚实的基础。
{"title":"Computer vision-based growth prediction and digestive tract assessment in pacific white shrimp (Litopenaeus vannamei)","authors":"Haocheng Zhao , Mei Liu , Ziwen Ren , Keyong Jiang , Xudong Zhao , Kefeng Xu , Yan Gao , Baojie Wang , Lei Wang","doi":"10.1016/j.aqrep.2025.103137","DOIUrl":"10.1016/j.aqrep.2025.103137","url":null,"abstract":"<div><div><em>Litopenaeus vannamei</em> is a key economic species in global aquaculture, and monitoring its growth is critical for optimizing feed management, and reducing costs. Traditional manual sampling methods are labor-intensive, error-prone, and can cause stress or injury to shrimp, negatively impacting growth. This study employed computer vision and machine learning to propose an innovative approach for growth prediction and digestive tract assessment in <em>L. vannamei</em>. An improved annotation method was developed to simplify the marking process and reduce weight prediction errors. Furthermore, A new length measurement approach, termed “visual total length”, was introduced to overcome the limitations of traditional measurement techniques. In this study, images of shrimp on feeding trays were analyzed to simulate real aquaculture monitoring conditions, and a prediction system was constructed by combining image segmentation (You Only Look Once v8n-seg, YOLOv8n-seg), classification (YOLOv8n-cls), traditional fitting, and machine learning models (Light Gradient Boosting Machine, LightGBM). The results showed that the improved annotation method also significantly reduced weight prediction errors caused by tail fan area. The visual total length had a high linear correlation with traditional total length (<em>r</em>² = 0.99), effectively allowing it to replace traditional measurements and enhancing applicability in real production environments. The final model achieved an accuracy of over 97 % in predicting length and weight when compared to manual measurements, and over 87 % accuracy in assessing digestive tract fullness. This study provides an efficient and precise method for growth monitoring, laying a solid foundation in future intelligent shrimp aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103137"},"PeriodicalIF":3.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-30DOI: 10.1016/j.aqrep.2025.103130
E.M. Kingma , M.C. Keur , S.T. Glorius, J.R. Graal, M.E.B. van Puijenbroek , R.W. Nauta
Food security is increasingly threatened by overfishing, climate change, and environmental degradation. Over-fishing depletes fish stocks, while climate change disrupts ecosystems and reduces arable land in coastal regions through salinization. As seafood demand rises, sustainable alternatives are needed. This study explores land-based cultivation of the common cockle (Cerastoderma edule) as a potential climate-resilient land use in salinized lowlands. Experiments were conducted in a field laboratory in the reclaimed Polder Wassenaar (Texel, Dutch Wadden Sea), using semi-controlled raceways under four water-flushing regimes. Growth and survival of one-year-old natural Wadden Sea cockles were monitored over the 2023 growing season. Cockles grew primarily from April to June, with an average shell length increase of 7.0 ± 1.0 mm (25.5 ± 2.8 %) by September. Higher flushing rates enhanced growth during the algal spring bloom, while condition indices peaked in May and declined post-spawning. At lower flushing rates, the building up of spawning reserves was likely limited by insufficient food input. Survival averaged 21.3 ± 2.7 %, although one raceway suffered mass mortality, reducing overall profitability. Nevertheless, other raceways achieved economic returns comparable to conventional Dutch agriculture. The findings highlight survival rates as the most important factor for economic viability, requiring improvements in stock quality, seeding time, and stress reduction. With further system optimization and higher stocking densities, high-density cockle farming may provide a profitable and climate-resilient alternative for salinized coastal regions where traditional agriculture is declining.
{"title":"Shell yeah! Cockle farming as alternative land use in salinized coastal lowlands − A case study on Texel, The Netherlands","authors":"E.M. Kingma , M.C. Keur , S.T. Glorius, J.R. Graal, M.E.B. van Puijenbroek , R.W. Nauta","doi":"10.1016/j.aqrep.2025.103130","DOIUrl":"10.1016/j.aqrep.2025.103130","url":null,"abstract":"<div><div>Food security is increasingly threatened by overfishing, climate change, and environmental degradation. Over-fishing depletes fish stocks, while climate change disrupts ecosystems and reduces arable land in coastal regions through salinization. As seafood demand rises, sustainable alternatives are needed. This study explores land-based cultivation of the common cockle (<em>Cerastoderma edule</em>) as a potential climate-resilient land use in salinized lowlands. Experiments were conducted in a field laboratory in the reclaimed Polder Wassenaar (Texel, Dutch Wadden Sea), using semi-controlled raceways under four water-flushing regimes. Growth and survival of one-year-old natural Wadden Sea cockles were monitored over the 2023 growing season. Cockles grew primarily from April to June, with an average shell length increase of 7.0 ± 1.0 mm (25.5 ± 2.8 %) by September. Higher flushing rates enhanced growth during the algal spring bloom, while condition indices peaked in May and declined post-spawning. At lower flushing rates, the building up of spawning reserves was likely limited by insufficient food input. Survival averaged 21.3 ± 2.7 %, although one raceway suffered mass mortality, reducing overall profitability. Nevertheless, other raceways achieved economic returns comparable to conventional Dutch agriculture. The findings highlight survival rates as the most important factor for economic viability, requiring improvements in stock quality, seeding time, and stress reduction. With further system optimization and higher stocking densities, high-density cockle farming may provide a profitable and climate-resilient alternative for salinized coastal regions where traditional agriculture is declining.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103130"},"PeriodicalIF":3.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-30DOI: 10.1016/j.aqrep.2025.103102
Jiangwei Li , Zhen Zhao , Ying Pan , Qinggan Xing , Haitao Ma , Chao Yue , Weitao Wan , Jun Li , Ziniu Yu , Yanping Qin , Yuehuan Zhang
Interspecific hybridization represents a significant approach for developing enhanced economic traits in aquatic species, and it was established in oyster breeding. However, early research has still needed to further deepen the understanding of the mechanisms of oyster hybridization advantages. Prior research has indicated that Crassostrea iredalei and C. dianbaiensis, as locally unique varieties, are used to hybridize and obtain excellent performance progeny, and analytical hybrid advantages may be representative. In this study, hybrid oyster DI (C. dianbaiensis ♀ × C. iredalei ♂) and its parents were subjected to long-term in situ tracking (180-day-old, 270-day-old, and 360-day-old), and comprehensively evaluated the dynamic regulation of physiological indicators and molecular levels related to hybrid dominance. Our findings revealed that hybrid progeny showed excellent performance in both growth and survival rate. In addition, the survival level of the DI population under different salinity stress is higher than that of its parents, which once again proves that DI has excellent phenotypic characteristics of heterosis. Transcriptome results from both tissues also support the gene expression pattern of the offspring approaching maternal genes. This maternal effect shows similar functional classifications in both the adductor muscle and the mantle, mainly reflected in the proliferation, metabolism, and immune resistance of cells, implying that the hybrid advantage of hybrid progeny comes from the main effect of the maternal parent. Simultaneously, genes exhibiting a positive-dominant expression pattern, originating from parental effector genes, have also been identified. Interestingly, candidate genes (ADAMTS16, FUCA1, PI4KA, SLC7A9, TCF7L2, and CSNK2A2) are considered to be markers related to growth traits in other aquatic animals. Candidate genes associated with immunomodulatory signaling pathways and resistance (SMAD4, MYD88, BIRC3, BIRC7, and PPTC7) were also screened. Notably, genes related to salinity adaptive regulation (SLC23A2 and SLC12A1) were screened, suggesting the potential and diversity of molecular regulation of heterosis. In short, hybrid oyster DI has obvious hybrid advantages in growth and stress resistance, POD genes, notably TCF7L2 and SLC12A1, are proposed as selectable markers for high-yield, stress-resilient oyster lines, and our findings provide the necessary theoretical perspective for a deep understanding of the hybrid advantages of oysters and their generation mechanism.
种间杂交是提高水生物种经济性状的重要途径，已在牡蛎育种中得到证实。然而，早期的研究还需要进一步加深对牡蛎杂交优势机制的认识。已有研究表明，作为当地特有的品种，长牡蛎和滇白牡蛎进行杂交得到了性能优异的后代，分析杂交优势可能具有代表性。本研究对杂交牡蛎DI （C. dianbaiensis♀× C. iredalei♂）及其亲本（180、270、360日龄）进行长期原位跟踪，综合评价杂种优势相关生理指标和分子水平的动态调控。结果表明，杂种后代在生长和成活率方面均表现优异。此外，DI群体在不同盐度胁迫下的存活水平均高于亲本，再次证明了DI具有优良的杂种优势表型特征。来自两个组织的转录组结果也支持后代接近母体基因的基因表达模式。这种母本效应在内收肌和套肌中都表现出相似的功能分类，主要体现在细胞的增殖、代谢和免疫抵抗等方面，说明杂交后代的杂交优势主要来自母本的作用。同时，还发现了来自亲本效应基因的正显性表达模式基因。有趣的是，候选基因（ADAMTS16、FUCA1、PI4KA、SLC7A9、TCF7L2和CSNK2A2）被认为是与其他水生动物生长性状相关的标记。筛选与免疫调节信号通路和耐药性相关的候选基因（SMAD4、MYD88、BIRC3、BIRC7和PPTC7）。值得注意的是，筛选到与盐度适应调控相关的基因（SLC23A2和SLC12A1），表明杂种优势分子调控的潜力和多样性。总之，杂种牡蛎DI在生长和抗逆性方面具有明显的杂交优势，POD基因，特别是TCF7L2和SLC12A1被提出作为高产抗逆性牡蛎品系的选择标记，我们的研究结果为深入理解牡蛎的杂交优势及其产生机制提供了必要的理论视角。
{"title":"Molecular insights into performance-related heterosis in offspring have been revealed by a hybrid oyster (Crassostrea dianbaiensis ♀ × C. iredalei ♂)","authors":"Jiangwei Li , Zhen Zhao , Ying Pan , Qinggan Xing , Haitao Ma , Chao Yue , Weitao Wan , Jun Li , Ziniu Yu , Yanping Qin , Yuehuan Zhang","doi":"10.1016/j.aqrep.2025.103102","DOIUrl":"10.1016/j.aqrep.2025.103102","url":null,"abstract":"<div><div>Interspecific hybridization represents a significant approach for developing enhanced economic traits in aquatic species, and it was established in oyster breeding. However, early research has still needed to further deepen the understanding of the mechanisms of oyster hybridization advantages. Prior research has indicated that <em>Crassostrea iredalei</em> and <em>C. dianbaiensis</em>, as locally unique varieties, are used to hybridize and obtain excellent performance progeny, and analytical hybrid advantages may be representative. In this study, hybrid oyster DI (<em>C. dianbaiensis</em> ♀ × <em>C. iredalei</em> ♂) and its parents were subjected to long-term in situ tracking (180-day-old, 270-day-old, and 360-day-old), and comprehensively evaluated the dynamic regulation of physiological indicators and molecular levels related to hybrid dominance. Our findings revealed that hybrid progeny showed excellent performance in both growth and survival rate. In addition, the survival level of the DI population under different salinity stress is higher than that of its parents, which once again proves that DI has excellent phenotypic characteristics of heterosis. Transcriptome results from both tissues also support the gene expression pattern of the offspring approaching maternal genes. This maternal effect shows similar functional classifications in both the adductor muscle and the mantle, mainly reflected in the proliferation, metabolism, and immune resistance of cells, implying that the hybrid advantage of hybrid progeny comes from the main effect of the maternal parent. Simultaneously, genes exhibiting a positive-dominant expression pattern, originating from parental effector genes, have also been identified. Interestingly, candidate genes (<em>ADAMTS16</em>, <em>FUCA1</em>, <em>PI4KA</em>, <em>SLC7A9</em>, <em>TCF7L2,</em> and <em>CSNK2A2</em>) are considered to be markers related to growth traits in other aquatic animals. Candidate genes associated with immunomodulatory signaling pathways and resistance (<em>SMAD4</em>, <em>MYD88</em>, <em>BIRC3</em>, <em>BIRC7</em>, and <em>PPTC7</em>) were also screened. Notably, genes related to salinity adaptive regulation (<em>SLC23A2</em> and <em>SLC12A1</em>) were screened, suggesting the potential and diversity of molecular regulation of heterosis. In short, hybrid oyster DI has obvious hybrid advantages in growth and stress resistance, POD genes, notably <em>TCF7L2</em> and <em>SLC12A1</em>, are proposed as selectable markers for high-yield, stress-resilient oyster lines, and our findings provide the necessary theoretical perspective for a deep understanding of the hybrid advantages of oysters and their generation mechanism.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"45 ","pages":"Article 103102"},"PeriodicalIF":3.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号