Aquaculture Reports最新文献

{"title":"A moderate dietary potassium diformate (KDF) is favorable for the growth, health status of intestine of juvenile rice field eel, Monopterus albus","authors":"Jinping Wu ,&nbsp;Lan Zhou ,&nbsp;Jiali Jin,&nbsp;Huamei Yue,&nbsp;Jing Zhang,&nbsp;Huan Ye,&nbsp;Chuangju Li,&nbsp;Rui Ruan","doi":"10.1016/j.aqrep.2026.103364","DOIUrl":"10.1016/j.aqrep.2026.103364","url":null,"abstract":"<div><div>To assess the effect of dietary potassium diformate (KDF) on intestine health of juvenile rice field eel, the biochemistry, histomorphology and microbiota properties of intestine were analyzed after an 8-week trial by feeding diets containing different levels of KDF (0.0 %, 0.3 %, 0.6 %, 0.9 %, 1.2 % and 1.5 % in the diets). The results indicated that specific growth rate (SGR) was not affected by the dietary KDF levels (<em>P</em> &gt; 0.05). Fish fed diet with KDF level at 0.9 % presented a significant improvement on digestive enzymes activity of the foregut and stomach compared with control group (0 % KDF) (<em>P</em> &lt; 0.05). The activities of intestine SOD, CAT, T-AOC and GSH-PX were significantly improved by the dietary KDF levels (<em>P</em> &lt; 0.05). Intestine MDA content in the 1.2 % KDF group was significantly lower than that in the 0.3 %, 0.6 %, and 0.9 % treatments (<em>P</em> &lt; 0.05). The significant elevation of AKP and ACP activities in intestine were observed in KDF supplementation groups (<em>P</em> &lt; 0.05). The fish in 0.9 % KDF group showed significantly more foregut intestinal folds than that of the other treatments (<em>P</em> &lt; 0.05). Inclusion of KDF in diets significantly increased the number of goblet cells in both the foregut and hindgut, and achieved the highest value at 0.9 % KDF group (<em>P</em> &lt; 0.05). No significant effect was found on the α-diversity indices of gut (<em>P</em> &gt; 0.05). At the phylum level, the intestinal microbiota was predominantly comprised of Proteobacteria, Firmicutes, Verrucomicrobiota, Fusobacteriota, Actinobacteriota, and Bacteroidota. The study revealed that an appropriate supplementation of KDF in diet is beneficial for the growth and intestinal health status of rice field eel by improving digestive enzymes activity, intestinal morphology and antioxidant capacity. The optimum supplementation level of KDF in juvenile rice field eel diet is determined at 0.88 g/kg based on SGR.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103364"},"PeriodicalIF":3.7,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of gender in quantifying the influence of online marketing on the performance of fish marketers in Southwest, Nigeria","authors":"Adewale Isaac Olutumise","doi":"10.1016/j.aqrep.2025.103326","DOIUrl":"10.1016/j.aqrep.2025.103326","url":null,"abstract":"<div><div>The rapid development of online markets, especially amid the global COVID-19 pandemic, has signified a fundamental change in the dynamics of markets and economies. This holds implications for various sectors, including fisheries and aquaculture. Despite the growing body of literature acknowledging the significance of online marketing, there exists a notable gap in understanding how online marketing influences the performance of marketers from the perspective of gender. Therefore, this research seeks to bridge this gap by conducting an empirical study on the role of gender in quantifying the influence of online market participation on the performance of fish marketers. Cross-sectional data were collected through a well-designed questionnaire. A multistage sampling technique was used to select 240 respondents. A budgetary technique was employed in measuring the performance. The empirical framework of the study considered potential endogeneity between online marketing and performance by using an endogenous treatment effects model with a linear outcome. The findings revealed that, even though fish marketing is profitable in the area, females make more profit than their male counterparts. It was also recorded that ignoring potential endogeneity may affect the statistical significance of the online market participation estimate and the direction of the influence of online marketing on the performance of fish marketers. The study revealed that online marketing had a direct impact on the performance of female and male fish marketers, with marginal effects of 3.65 and 1.98, respectively. Variables such as education, access to smartphones, digital literacy, market size, and internet availability are significant determinants of online market participation in the area. Therefore, our findings support the literature that online marketing significantly increases performance. Internet infrastructure should be improved and made affordable to allow more users, and this could be designed according to the characteristics of different groups to promote the marketers’ livelihoods.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103326"},"PeriodicalIF":3.7,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental protocol for bath infection of Atlantic salmon with Tenacibaculum dicentrarchi: Aiming for target mortalities","authors":"Almendra Benavides ,&nbsp;Ruth Montero ,&nbsp;Roque Scarpa ,&nbsp;Marcos Mancilla ,&nbsp;Margareth Øverland ,&nbsp;Mónica Imarai","doi":"10.1016/j.aqrep.2025.103345","DOIUrl":"10.1016/j.aqrep.2025.103345","url":null,"abstract":"<div><div>Developing experimental protocols to establish infection models in fish is essential for identifying new and effective preventive tools to improve fish health and welfare. Tenacibaculosis, caused by <em>Tenacibaculum</em> species, is a significant concern, particularly for Atlantic salmon (<em>Salmo salar</em>). However, replicable infection models for studying this disease remain limited. In this study, we developed a reliable bath infection method for <em>T. dicentrarchi</em>, enabling targeted, controlled mortality rates. First, using flow cytometry, we accurately quantified viable bacteria that were subsequently used for bath infection. A consistent mortality of ∼24 % was achieved across experiments, even when scaling up bath infection volumes. Infected fish displayed characteristic symptoms, including ulcers, oral erosions, and hemorrhages, and PCR confirmed bacterial presence. This protocol improved reproducibility in experimental infections with <em>T. dicentrarchi</em>, supporting research on new prophylactic methods and treatments against Tenacibaculosis.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103345"},"PeriodicalIF":3.7,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145972732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving intestinal health benefits by supplementation with emodin in juvenile largemouth bass (Micropterus salmoides): Intestinal morphology, digestion, microbiota and antioxidant capacity","authors":"Zhenxin Zhao ,&nbsp;Beiping Tan ,&nbsp;Shuyan Chi ,&nbsp;Qihui Yang","doi":"10.1016/j.aqrep.2025.103352","DOIUrl":"10.1016/j.aqrep.2025.103352","url":null,"abstract":"<div><div>Our study investigated the effects of dietary emodin on intestinal morphology, digestive function, gut microbiota, and antioxidant capacity in juvenile largemouth bass. A total of 540 fish (45 ± 0.3 g) were randomly assigned to six dietary treatments (EM0, EM250, EM500, EM1000, EM2000, and EM4000), corresponding to emodin supplementation levels of 0, 250, 500, 1000, 2000, and 4000 mg / kg, respectively. After a two-month feeding trial, emodin supplementation at 500–2000 mg / kg significantly increased intestinal villus height (VH), villus width (VW), and goblet cell (GC) count compared with the EM0 group (<em>P</em> &lt; 0.05). In contrast, the 4000 mg / kg dose significantly reduced VH and GC counts (<em>P</em> &lt; 0.05). Activities of digestive enzymes, including amylase, alkaline phosphatase (AKP), creatine kinase (CK), gamma-glutamyl transpeptidase (GGT), lipase, and pepsin, were significantly enhanced in the EM1000 group (<em>P</em> &lt; 0.05). Furthermore, emodin supplementation improved the composition, diversity, and richness of the intestinal microbiota in a dose-dependent manner. Specifically, EM1000 decreased the relative abundance of potential pathogens (<em>Fusobacteriaceae</em> and <em>Vibrionaceae</em>) and increased the abundance of potential probiotics (<em>Rubrobacteriaceae</em> and <em>Lactobacillaceae</em>) (<em>P</em> &lt; 0.05). This group further showed enhanced intestinal antioxidant and anti-inflammatory capacity, characterized by the elevated expression of catalase (<em>CAT</em>), heme oxygenase-1 (<em>HO-1</em>), superoxide dismutase (<em>SOD</em>), and nuclear factor erythroid 2-related factor 2 (<em>Nrf2</em>), along with reduced expression of the pro-inflammatory cytokines interleukin-8 (<em>IL-8</em>) and tumor necrosis factor-alpha (<em>TNF-α</em>) (<em>P</em> &lt; 0.05). In summary, dietary emodin at 1000 mg / kg optimally modulated the gut microbiota and improved intestinal digestion, histomorphology, and non-specific immunity in juvenile largemouth bass.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103352"},"PeriodicalIF":3.7,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary vitamin E supplementation on growth performance and liver health of Jinhu grouper (Epinephelus fuscoguttatus ♀ × Epinephelus tukula ♂) fed oxidized fish oil diets","authors":"Guoyong Huang ,&nbsp;Weibin Huang ,&nbsp;Guangcai Fu ,&nbsp;Yansheng Liu ,&nbsp;Hengyang Song ,&nbsp;Wenshan Cai ,&nbsp;Yubin Liu ,&nbsp;Yuxuan Han ,&nbsp;Yilin Yao ,&nbsp;Beiping Tan ,&nbsp;Xiaohui Dong","doi":"10.1016/j.aqrep.2025.103309","DOIUrl":"10.1016/j.aqrep.2025.103309","url":null,"abstract":"<div><div>This study assessed the efficacy of dietary vitamin E in ameliorating growth and liver function in Jinhu grouper compromised by the inclusion of oxidized fish oil (OFO; peroxide value: 121.68 mmol/kg) in the diet, compared with a fresh fish oil control (FFO; peroxide value: 1.25 mmol/kg). The eight-week feeding trial utilized five experimental diets that were isonitrogenous and isolipidic: FFO, OFO (0 mg/kg VE), or OFO supplemented with 200 (VE1), 400 (VE2), or 600 (VE3) mg/kg vitamin E. Fish receiving oxidized fish oil showed slower growth, indicated by reduced weight gain rate (WGR) and increased feed conversion ratio (FCR). Intake of oxidized lipids altered lipid metabolism, lowering serum total cholesterol (TC), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C) but elevating low-density lipoprotein cholesterol (LDL-C) and malondialdehyde (MDA) levels. Hepatic antioxidant capacity was compromised, as revealed by the concurrent suppression in both the activities and gene expression levels of key antioxidant enzymes (SOD, CAT, and GPx). Concurrently, oxidized fish oil induced liver inflammation, significantly upregulating pro-inflammatory cytokine genes (notably <em>il-1β</em>, <em>il-6</em>, <em>il-8</em>, <em>tnf-α</em>), and caused histopathological damage including hepatocyte nuclear displacement and lipid droplet accumulation. Vitamin E supplementation ameliorated these effects. The VE2 group (400 mg/kg) maximally restored growth performanc, reduced serum MDA and LDL-C levels, enhanced hepatic antioxidant enzyme activities and gene expression, suppressed inflammatory cytokine expression, and attenuated histopathological lesions. In conclusion, dietary vitamin E supplementation at 400 mg/kg effectively counteracts growth impairment and hepatotoxicity induced by oxidized fish oil in Jinhu grouper.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103309"},"PeriodicalIF":3.7,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined effects of glutamine dipeptide, plant essential oils, and Astragalus polysaccharide on growth performance, antioxidant capacity, immune function, and intestinal health in large yellow croaker (Larimichthys crocea)","authors":"Xiang Zhu ,&nbsp;Yu Zhan ,&nbsp;Yanting Chen ,&nbsp;Ying Hang ,&nbsp;Chaoxin Wang ,&nbsp;Shuichao Mi ,&nbsp;Jun Zhang ,&nbsp;Minqiu Sun ,&nbsp;Lihua Yuan ,&nbsp;Manshan Lin ,&nbsp;Saiya Liu ,&nbsp;Bin Luo ,&nbsp;Dong Yu ,&nbsp;Xueming Hua ,&nbsp;Chuanming Zhong","doi":"10.1016/j.aqrep.2025.103283","DOIUrl":"10.1016/j.aqrep.2025.103283","url":null,"abstract":"<div><div>As the most productive fish species in marine aquaculture in China, large yellow croaker (<em>Larimichthys crocea</em>) farming faces dual challenges: the frequent outbreak of diseases and increasing environmental pressures. While high-dose antibiotic treatments may offer short-term disease control, they contribute significantly to the development of drug resistance. Consequently, the development of eco-friendly and safe immune enhancers, along with the implementation of nutritional intervention strategies, has become a focal point of current research. This study investigated the combined effects of dietary supplementation with glutamine dipeptide (GLN), plant essential oil (PEO), and <em>Astragalus</em> polysaccharide (APS) on growth performance, antioxidant capacity, immune function, and gut microbiota of <em>L. crocea</em>. A 60-day feeding trial was conducted in sea cages with four dietary groups: control (basal diet), GLN (2 %), GLN+PEO (2 % GLN + 0.55 % PEO), and PEO+APS (0.55 % PEO + 0.1 % APS). Results indicated that GLN+PEO significantly improved the survival rate and enhanced liver antioxidant enzyme activities (CAT, GSH-PX, T-AOC) (<em>P</em> &lt; 0.05). PEO+APS showed the strongest systemic antioxidant effect, reducing lipid peroxidation in serum and intestine while elevating lysozyme activity (<em>P</em> &lt; 0.05). GLN alone protected against hepatic oxidative damage but had limited growth-promoting effects. Gut microbiota analysis revealed distinct restructuring, with PEO+APS maintaining higher microbial diversity and enriching beneficial genera (<em>Faecalibacterium</em>, <em>Bifidobacterium</em>), while GLN+PEO reduced microbial richness. These findings demonstrate that combinatorial additives (especially PEO+APS combination) offer superior benefits for enhancing health and stress resistance in intensive aquaculture, providing a sustainable alternative to antibiotics.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103283"},"PeriodicalIF":3.7,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive whole transcriptome analysis reveals specific lncRNA, circRNA and miRNA-mRNA networks of pearl oyster (Pinctada fucata martensii) in response to hypoxia stress","authors":"Qian Wu ,&nbsp;Jiawei Zhang ,&nbsp;Lintao Liu ,&nbsp;Chuangye Yang ,&nbsp;Yongshan Liao ,&nbsp;Qingheng Wang ,&nbsp;Yuewen Deng","doi":"10.1016/j.aqrep.2025.103306","DOIUrl":"10.1016/j.aqrep.2025.103306","url":null,"abstract":"<div><div>Hypoxia is a prevalent environmental stressor in both natural and aquaculture environments. <em>Pinctada fucata martensii</em>, a commercially valuable pearl oyster species, is particularly susceptible to hypoxic stress in aquaculture. To explore its molecular response to short-term hypoxia, whole-transcriptome sequencing was performed on gill tissues under normoxic and hypoxic conditions. A total of 721 differentially expressed mRNAs (DEmRNAs), 259 long non-coding RNAs (DElncRNAs), 55 circular RNAs (DEcircRNAs), and 17 microRNAs (DEmiRNAs) were identified. Based on predicted interactions among these differentially expressed RNAs, a competing endogenous RNA (ceRNA) network was constructed, consisting of 9 DElncRNAs, 10 DEcircRNAs, 3 DEmiRNAs, and 6 DEmRNAs. Six DElncRNAs and three DEcircRNAs were predicted to interact with miR-152, and the axis-mediated activation of target genes—ALDH5A1 and SLC23A2, FAT4 and CCDC97, and LAC—was implicated in the modulation of metabolic pathways, enzyme activity, antioxidant defenses, thereby promoting antioxidant capacity and suppressing cell growth as part of an adaptive survival strategy. This ceRNA network reveals a key molecular hub that links hypoxia sensing to immunomodulatory mechanisms. Moreover, the co-repression of stress-response genes HSP20 and HSP70 in this network enhances hypoxic tolerance increased energy utilization efficiency and regulation of RNA. Functional analysis of the network revealed enrichment in pathways related to energy metabolism, antioxidant defense, and RNA splicing regulation. These findings provide novel insights into transcriptomic regulation and adaptive mechanisms in bivalves under hypoxic stress.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103306"},"PeriodicalIF":3.7,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated multi-omics analysis of muscle identifies H3K4me3 epigenetic regulation of growth traits in pacific white shrimp (Litopenaeus vannamei)","authors":"Xin Zhang ,&nbsp;Pengying Li ,&nbsp;Bo Ma ,&nbsp;Chunhua Ren ,&nbsp;Chaoqun Hu ,&nbsp;Ting Chen ,&nbsp;Peng Luo","doi":"10.1016/j.aqrep.2025.103305","DOIUrl":"10.1016/j.aqrep.2025.103305","url":null,"abstract":"<div><div>The Pacific white shrimp (<em>Litopenaeus vannamei</em>) is the predominant aquaculture species worldwide, and China has the highest production value in its cultivation. Growth traits are widely acknowledged as the most critical economic characteristics in shrimp farming. Genome-wide association study (GWAS) is a tool to dissect the genetic basis of the target traits. Histone methylation, particularly trimethylated H3 lysine 4 (H3K4me3), plays a pivotal role as a posttranslational modification that regulates gene expression by influencing chromatin compaction. In this research, we conducted a genome-wide association study (GWAS) on <em>L. vannamei</em>. Through this in-depth analysis, we successfully identified several genes associated with fast-growth traits. Notably, a histone methyltransferase (HMT) gene presented significantly different expression levels between shrimp with distinct growth phenotypes. To gain a more comprehensive understanding of the histone modification mechanisms underlying these growth disparities, we employed CUT &amp; Tag analysis. A total of 165 differential peaks (DPs) were identified, comprising 75 DPs related to gain (gain DPs) and 90 DPs related to loss (loss DPs). Gene annotation was performed on these classified DPs, and a total of 146 differential peaks genes (DPGs) were found. Among them, 65 were gained DPGs, and 81 were lost DPGs, which were involved in pathways such as glycolysis/gluconeogenesis, carbon metabolism, and nitrogen metabolism. By integrating the CUT &amp; Tag results with our previous RNA-seq data, we identified 18 differentially expressed genes (DEGs) that were associated with H3K4me3 modification. Specifically, 12 DEGs with loss DPs (down-regulated) and 6 DEGs with gain DPs (up-regulated). Key genes such as <em>triose-phosphate isomerase</em> (<em>TPI</em>), <em>4-α-glucanotransferase</em> (<em>4αGTs</em>), <em>glucose-6-phosphate isomerase</em> (<em>GPI</em>), and <em>purine nucleoside phosphorylase</em> (<em>PNPase</em>) were also identified. The presence of these genes strongly suggests their crucial contributions to nutrient absorption and cell growth. This study not only successfully identified multiple functional genes related to growth traits through GWAS but also comprehensively investigated the role of histone modification in regulating shrimp growth. By integrating CUT &amp; Tag analysis with transcriptome data analysis, our research offers initial yet valuable insight into the H3K4me3 modification regulatory mechanisms governing growth performance in <em>L. vannamei</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103305"},"PeriodicalIF":3.7,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reducing nitrogen and phosphorus discharges from sea cucumber-based recirculating aquaculture system through dietary binder addition","authors":"Yijing Zhou ,&nbsp;Chenyu Song ,&nbsp;Qiang Li ,&nbsp;Lingxiang Du ,&nbsp;Xian Li ,&nbsp;Dengpan Dong ,&nbsp;Zhitao Huang ,&nbsp;Meng Li ,&nbsp;Xiefa Song","doi":"10.1016/j.aqrep.2025.103299","DOIUrl":"10.1016/j.aqrep.2025.103299","url":null,"abstract":"<div><div>The high dispersibility of dietary and fecal particles aggravates excessive nitrogen (N) and phosphorus (P) discharge in <em>Apostichopus japonicus</em> recirculating aquaculture system (RAS). This study evaluated the feasibility of optimizing N&amp;P budgets and water quality through binder-enhanced dietary management. The experimental groups included a control without binder addition and treatments with 5 % of one of four binders: sweet potato starch, xanthan gum, guar gum and carrageenan. This study determined the effects of dietary binders on aquaculture water quality, sea cucumber growth, and the properties (viscosity and particle size) of feed and feces, analyzed the N&amp;P flow in the system based on the principle of mass balance, and applied Spearman correlation analysis to explore the relationships among viscosity, particle size, and N&amp;P budgets. The results showed that the guar gum supplementation significantly reduced total nitrogen and total phosphorus concentrations in aquaculture water by 24.8 % and 38.5 %, respectively, with a significant reduction in ammonia nitrogen concentration (0.08–0.18 mg/L) (<em>P</em> &lt; 0.05). Binders modified sediment-water distribution of N/P components, increasing the retention of N and P by solids while inhibiting the release of dissolved N and P. Mechanistically, sweet potato starch and guar gum optimized dietary/fecal viscosity, enlarged particle size (Dx(50)) and enhanced structural stability, thus promoting nutrient immobilization in bioavailable solid forms. The findings of this study validate binder-assisted dietary management as a dual-functional solution, enhancing aquaculture productivity while alleviating environmental pressure in RAS through targeted nutrient retention.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103299"},"PeriodicalIF":3.7,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the microRNA regulatory network underlying the planktonic-to-benthic transition in the Pacific oyster","authors":"Yusu Xie ,&nbsp;Mingyu Yang ,&nbsp;Haiming Sun ,&nbsp;Fei Xu","doi":"10.1016/j.aqrep.2025.103304","DOIUrl":"10.1016/j.aqrep.2025.103304","url":null,"abstract":"<div><div>The Pacific oyster, <em>Crassostrea gigas</em>, undergoes a critical developmental transition from planktonic larvae to sessile benthic juveniles, a process essential for both aquaculture productivity and the stability of marine ecosystems. While environmental and neuroendocrine cues regulating this transition have been extensively studied, the contribution of microRNAs (miRNAs) remains largely unexplored. In this study, we employed a high-resolution temporal sampling strategy to investigate oyster metamorphosis, identified 33 miRNAs associated with larval settlement and metamorphosis. Additionally, we uncovered 35 highly correlated miRNA-target gene pairs, revealing a complex post-transcriptional regulatory network. We further validated the expression pattern of the potential regulatory hub miRNA novel_mir_80 and confirmed its presence through whole-mount in situ hybridization using LNA-modified probes. Functional annotation of target genes highlighted key roles in adhesion, signal transduction, protein homeostasis, cytoskeleton remodeling, genome stability, and transcriptional and epigenetic regulation. These results underscore the pivotal role of miRNAs in orchestrating developmental reprogramming during the planktonic-benthic transition. Our findings not only expand current understanding of miRNA-mediated gene regulation in <em>C. gigas</em>, but also provide novel molecular targets with potential applications in oyster breeding and aquaculture management.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103304"},"PeriodicalIF":3.7,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}